CN105919127B - A kind of stable high anthocyanin blueberry buccal tablets and preparation method thereof - Google Patents
A kind of stable high anthocyanin blueberry buccal tablets and preparation method thereof Download PDFInfo
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- CN105919127B CN105919127B CN201610267232.2A CN201610267232A CN105919127B CN 105919127 B CN105919127 B CN 105919127B CN 201610267232 A CN201610267232 A CN 201610267232A CN 105919127 B CN105919127 B CN 105919127B
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- 235000017537 Vaccinium myrtillus Nutrition 0.000 title claims abstract description 236
- 235000003095 Vaccinium corymbosum Nutrition 0.000 title claims abstract description 235
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- 240000000851 Vaccinium corymbosum Species 0.000 title claims abstract description 163
- 235000010208 anthocyanin Nutrition 0.000 title claims abstract description 115
- 229930002877 anthocyanin Natural products 0.000 title claims abstract description 115
- 239000004410 anthocyanin Substances 0.000 title claims abstract description 115
- 150000004636 anthocyanins Chemical class 0.000 title claims abstract description 115
- 239000006189 buccal tablet Substances 0.000 title claims abstract description 44
- 238000002360 preparation method Methods 0.000 title claims abstract description 28
- 244000077233 Vaccinium uliginosum Species 0.000 claims abstract description 73
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- 239000000287 crude extract Substances 0.000 claims abstract description 29
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- 244000283207 Indigofera tinctoria Species 0.000 claims description 3
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- DKXULEFCEORBJK-UHFFFAOYSA-N magnesium;octadecanoic acid Chemical compound [Mg].CCCCCCCCCCCCCCCCCC(O)=O DKXULEFCEORBJK-UHFFFAOYSA-N 0.000 description 2
- 229910021645 metal ion Inorganic materials 0.000 description 2
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
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- 229930014669 anthocyanidin Natural products 0.000 description 1
- 150000001453 anthocyanidins Chemical class 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The present invention provides a kind of stable high anthocyanin blueberry buccal tablets and preparation method thereof, belong to lozenge preparation technical field, wherein, stable high anthocyanin blueberry buccal tablets of the present invention include following component: stable Anthocyanin from Blueberry crude extract, blueberry polyoses extract, microcrystalline cellulose, mannitol, citric acid, magnesium stearate and blueberry ethanol solution；Simultaneously, provide a kind of preparation method of stable high anthocyanin blueberry buccal tablets, it include: blueberry mashing, centrifuge separation, the preparation of blueberry ethanol solution, ultrasonic extraction blueberry pomace, centrifugation Anthocyanin from Blueberry extracting solution, freeze-drying, embedding Anthocyanin from Blueberry crude extract, mixing formula, granulation, drying, sterilizing, tabletting, what the present invention innovated fully utilizes all effective components in blueberry, the blueberry buccal tablets being finally made have anthocyanin content high, anthocyanin storage is stablized, and lozenge is full of nutrition, clean taste, the features such as being convenient for carrying.
Description
Technical field
The present invention relates to a kind of lozenges and preparation method thereof, and in particular to a kind of stable high anthocyanin blueberry buccal tablets and its
Preparation method.
Background technique
Blueberry be Ericaceae (Ericaceae) genus vaccinium (Vaccinium spp) plant also known as blueberry, bilberry and
Blue berry etc. is evergreen or machaka type fruit tree.Currently, extensive introducing and planting, fruit are approximately blueberry in the world
Circle, pericarp are blue, and pulp is fine and smooth, by public pro-gaze.In addition to being rich in carbohydrate, fat, albumen in Blueberry
Outside the various bioactive components such as matter, fat, vitamin, microelement and flavones, tannin, phenolic acid, anthocyanin is also blueberries
Real one of main composition.
Anthocyanin from Blueberry is a kind of water-soluble natural edible pigment widely distributed in nature, safe and nontoxic.Modern medicine
Pharmacological research shows that anthocyanin can obviously improve eyesight, promote cardio-pulmonary function, prevents cardiovascular and cerebrovascular disease, infectious diseases, prolongs
Slow skin aging has the effects that anticancer, antitumor, is known as after water, protein, fat, carbohydrate, vitamin, mine
The seventh-largest essential nutrients after substance.Nowadays, since Anthocyanin from Blueberry has apparent health-care efficacy, so that being rich in pattern
The food of glycosides is continuously added in food as nutrition intensive.
Lozenge is to be contained in the tablet for being slowly dissolved in oral cavity and playing lasting local therapeutic effects.It has simple process,
Convenient, the advantages that stability is good, drug effect is rapid, absorption is fast and bioavilability is high, in good taste, be both health food and
It is snack food.The preparation process of lozenge generally comprise raw material mixing, softwood processed, granulation and drying, whole grain, tabletting, sterilizing and
Packaging.Anthocyanin from Blueberry can make the change of the external environments such as oxygen, oxidant, affinity agent, enzyme, metal ion, temperature, illumination
Its nutrition and physiological function is caused to decline at the change of Anthocyanin from Blueberry structure.Lozenge is eaten as a kind of health care of permanent preservation
Product and snack food, granulation, dry and sterilization technological temperature are higher, be easy to cause ingredient to be destroyed, especially anthocyanin
It is this to be easily oxidized unstable ingredient；More importantly the other compositions in the storage shelf life of lozenge, in lozenge
Do not change, and the anthocyanin in lozenge easily due to oxygen, oxidant, affinity agent, enzyme, metal ion, temperature, illumination etc. because
Element decomposes denaturation, loses the biological physiologies function such as its health care, causes the loss of primary healthcare ingredient in blueberry buccal tablets.
In order to solve in blueberry buccal tablets Anthocyanin from Blueberry content because in preparation process and storage can cause anthocyanin steady
The problem of qualitative decline, the present invention provides a kind of blueberry buccal tablets of high anthocyanin content, and after storage a period of time, blueberry contains
Anthocyanin content loss in piece greatly reduces.
Summary of the invention
The present invention provides a kind of high anthocyanin blueberry buccal tablets and preparation method thereof, and the present invention is accomplished by the following way:
Wherein, the component of stable high anthocyanin blueberry buccal tablets provided by the invention includes: that stable Anthocyanin from Blueberry is thick
Extract, blueberry polyoses extract, microcrystalline cellulose, mannitol, citric acid, magnesium stearate and blueberry ethanol solution.
Further, the formula of the stable high anthocyanin blueberry buccal tablets is inventor provided, it is specific as follows: stable indigo plant
5 ~ 25 parts of certain kind of berries anthocyanin crude extract, 10 ~ 20 parts of blueberry polyoses extract, 5 ~ 20 parts of microcrystalline cellulose, 5 ~ 15 parts of mannitol, lemon
2 ~ 5 parts, 5 ~ 10 parts of magnesium stearate, 5 ~ 20 parts of blueberry ethanol solution of acid.
Meanwhile the present invention provides a kind of preparation method of stable high anthocyanin blueberry buccal tablets, the preparation method is specific
The following steps are included:
A. blueberry is beaten: by Blueberry crushing and beating, after adding pectase, homogenate digests 1 ~ 3h, obtains blueberries
Slurry, is added citric acid in blueberry pulp；
B. be centrifugated: by blueberry pulp centrifugal treating, after centrifugation, blueberry pulp is separated into blueberry juice and blueberry pomace；
C. the preparation of blueberry ethanol solution: by blueberry juice according to 1:2(v/v) and dehydrated alcohol mixing, obtain blueberry ethyl alcohol
Solution；
D. blueberry pomace ultrasonic extraction: by blueberry pomace in the ethanol water of pH=2.5 ~ 3.5 ultrasonic extraction 20-30
Min obtains Anthocyanin from Blueberry extracting solution；
E. it is centrifuged Anthocyanin from Blueberry extracting solution: by Anthocyanin from Blueberry extracting solution centrifugal treating, taking filtrate after filtering, obtain indigo plant
Certain kind of berries anthocyanin extracting solution, filter residue is freeze-dried to obtain blueberry polyoses extract；
F. it is freeze-dried: Anthocyanin from Blueberry extracting solution being freeze-dried, Anthocyanin from Blueberry crude extract is obtained；
G. it embeds Anthocyanin from Blueberry crude extract: Anthocyanin from Blueberry crude extract is added in carboxymethyl chitosan solution,
After being thoroughly mixed under the action of magnetic stirring apparatus, chitosan hydrochloride solution is added dropwise, after reacting 1h, lower sediment is collected in centrifugation,
It is freeze-dried 12-16h, obtains stable Anthocyanin from Blueberry crude extract；
H. raw material mixes: take stable Anthocyanin from Blueberry crude extract, blueberry polyoses extract, and microcrystalline cellulose, mannitol,
Citric acid, magnesium stearate are sufficiently mixed, and blueberry ethanol solution is added, blueberry buccal tablets softwood is made；
I. it is granulated and dry: blueberry buccal tablets softwood being put into granulator and is granulated, and wet granular is set into drying process, is obtained
To blueberry particle；
J. it sterilizes: blueberry particle is irradiated into 10 ~ 40min sterilizing under ultraviolet light；
K. tabletting: by blueberry particle tabletting in gnotobasis of sterilizing, packaging obtains stable high anthocyanin blueberry and contains
Piece finished product.
Further, in the step A, the additive amount of pectase is 0.1 ~ 3mg/g.
Further, the ultrasonic power of the blueberry pomace ultrasonic extraction are as follows: 300-400W, Extracting temperature are as follows: 50 ~ 60 DEG C.
Further, the concentration of the carboxymethyl chitosan solution is 2.0-3.0 mg/mL；The chitosan hydrochloride solution
Concentration be 0.6-1.2 mg/mL.
Beneficial effect
First,
Firstly, the present invention is a kind of lozenge pure natural, without side-effects.Other than the basic additive of production lozenge, greatly
For part material in blueberry, prepared blueberry buccal tablets itself, anthocyanin content relatively up to arrives 0.73mg/g, is preparing
Specially and the anthocyanin in blueberry is effectively protected in journey, anthocyanin content containing close to blueberry itself in lozenge can be made
Amount, entire preparation step greatly reduce anthocyanin loss；Meanwhile it has been found that blueberry buccal tablets prepared by the present invention flower
Color glycosides by long-time store after, content because attenuation by oxygen speed significantly reduce, Acceleration study the results show that the present invention can
So that the stability of anthocyanin promotes 3 times in blueberry buccal tablets.By blueberry juice according to 1:2(v/v) and dehydrated alcohol mixing, it obtains
The anthocyanin in blueberry buccal tablets can be effectively protected in blueberry ethanol solution, there is important influence to the content of final anthocyanin.
Secondly, instantly, functional food has become the hot subject of food industry, and anthocyanin is a kind of safety, nothing
The water-soluble food pigment of poison, belongs to flavones polyphenol compound, has stronger anti-oxidant and Scavenging ability, pattern
Glycosides is retained in blueberry buccal tablets, can not only be improved eyesight, delay skin aging, be promoted cardiopulmonary, and can prevent some chronic diseases
Disease, such as cardiovascular disease, cancer.
Finally, it is raw material that the present invention, which selects blueberry, it is higher that anthocyanin content can be obtained by ultrasonic wave assisted extraction method
Extracting solution obtain the crude extract of Anthocyanin from Blueberry using freeze-drying, be aided with citric acid etc. seasoning protective ingredient, develop
Provide the blueberry buccal tablets for improving the functions such as eyesight, prevention chronic disease.Citric acid is a kind of common food additives, can be increased
Strong internal eubolism, promotes appetite.Secondly, anthocyanin is more stable in acid condition, pattern is can be improved in addition organic acid
The stability of glycosides.The mouthfeel and flavor of lozenge are improved simultaneously.
Second,
The present invention is using blueberry as primary raw material, and in the preparation process of blueberry buccal tablets, innovation is fully utilized in blueberry
All effective components, the blueberry buccal tablets being finally made have anthocyanin content high, and anthocyanin storage is stablized, lozenge is full of nutrition,
Clean taste is convenient for carrying, and the effective component in blueberry residue is extracted recycling again again in particular with ultrasonic wave extraction
It utilizes, turns waste into wealth, fully utilize the various nutritional ingredients of blueberries to the maximum extent, improve the whole benefit of Blueberry
With value, economic benefit is increased.
Operation of the present invention is simple, not high to equipment requirement, and production cost is low, the anthocyanin contents of obtained blueberry buccal tablets compared with
Height, anthocyanin content is higher after long-time is stored, and blueberry buccal tablets yield is higher, economical and practical, before having good market
Scape.
Specific embodiment.
Embodiment 1
A kind of high anthocyanin blueberry buccal tablets, raw material include: blueberry, chitosan, microcrystalline cellulose, mannitol, citric acid
And magnesium stearate.
Preparation method specifically includes the following steps:
1) blueberry is beaten: by Blueberry crushing and beating, after adding 0.1 mg/g pectase, and homogenate, enzymolysis time is 1 ~
3h after the completion of enzymatic hydrolysis, obtains blueberry pulp, and the citric acid of blueberry pulp quality 0.38% is added；
2) be centrifugated: blueberry pulp is separated into blueberry juice and blueberry pomace by centrifuge, revolving speed 3000 ~
Under conditions of 5000r/min, it is centrifuged 10 ~ 20min；
3) preparation of blueberry ethanol solution: by blueberry juice according to 1:2(v/v) and dehydrated alcohol mixing, obtain blueberry ethyl alcohol
Solution；
4) by blueberry pomace according to solid-liquid ratio 1:20g/mL, pH=3.0,60%(v/v blueberry pomace ultrasonic extraction: are added) second
Alcohol solution, while under conditions of ultrasonic power 300-400W, 50 ~ 60 DEG C of Extracting temperature, ultrasonic extraction 20-30 min is obtained
To Anthocyanin from Blueberry extracting solution；
5) it is centrifuged Anthocyanin from Blueberry extracting solution: by Anthocyanin from Blueberry extracting solution centrifugal treating, in revolving speed 3800r/min condition
Under, it is centrifuged 15min, filtrate is taken after filtering, obtains Anthocyanin from Blueberry extracting solution, filter residue is freeze-dried to obtain blueberry Polyose extraction
Object；
6) it is freeze-dried: Anthocyanin from Blueberry extracting solution being freeze-dried, Anthocyanin from Blueberry crude extract is obtained.
7) embed Anthocyanin from Blueberry crude extract: it is 2.0- that 15 mL concentration, which are added, in 5-10mg Anthocyanin from Blueberry crude extract
In 3.0 mg/mL carboxymethyl chitosan solutions, under magnetic stirring apparatus effect, after reacting 15min, it is slowly added to 30mL dropwise
Concentration is 0.6-1.2 mg/mL chitosan hydrochloride solution, under the action of magnetic stirring apparatus, after reacting 1h, and in revolving speed 10,
Under the conditions of 000 r/min, it is centrifuged 10min, collects lower sediment.It is freeze-dried 12-16h, it is thick to obtain stable Anthocyanin from Blueberry
Extract.
8) raw material mixes: taking stable Anthocyanin from Blueberry crude extract 10g, blueberry polyoses extract 15g, microcrystalline cellulose
20g, mannitol 10g, citric acid 2g, magnesium stearate 8g are sufficiently mixed, and are added and are added 10mL blueberry ethanol solution, are made
Blueberry buccal tablets softwood.
9) it is granulated and dry: blueberry buccal tablets softwood being put into oscillating granulator and is granulated, wet granular is placed in 30
Dry in DEG C air dry oven, 20min is stirred once, and the moisture content in particle is down to 3%, obtains blueberry particle.
10) it sterilizes: blueberry particle is irradiated into 10 ~ 40min sterilizing under ultraviolet light.
11) it tabletting: by blueberry particle tabletting in gnotobasis of sterilizing, and is packed in time, obtains high anthocyanin
Blueberry buccal tablets finished product.
Applicant is using the anthocyanin in HPLC method detection lozenge, the specific steps are as follows:
1g lozenge is accurately weighed, is settled to distilled water in the volumetric flask of 10 mL, then with 0.45 um filtering with microporous membrane
Afterwards, side is examined with HPLC.
HPLC condition:
A phase: 0.05% formic acid water, B phase: acetonitrile, sample introduction flow velocity: column temperature: 1.0 mL/min 35.0 °C, use ultraviolet inspection
Survey device, wavelength 510nm, using gradient elution
Table 1: condition of gradient elution
Draw cyaniding 3-0-glucoside standard curve:
It accurately weighs 10 mg cyaniding 3-0-glucoside standard items, in constant volume and 10 mL volumetric flasks, obtains 1
Mg/mL cyaniding 3-0-glucoside mother liquor；Prepare respectively 0.02 mg/mL, 0.04 mg/mL,
The standard solution of 0.06 mg/mL, 0.08 mg/mL detect anthocyanin using above-mentioned HPLC method, obtain pattern
Glycosides standard curve: Y=2*107 X-1413.9 R2=0.9999
Through detecting, after 1 blueberry buccal tablets of the embodiment of the present invention store 7 days in 40 DEG C of constant incubators, anthocyanin retains
Rate is 39.47%, and in the Anthocyanin from Blueberry lozenge of other methods production, anthocyanin retention rate is 10.81%.
Under 40 DEG C of holding conditions, the variation of anthocyanidin content
Embodiment 2
A kind of high anthocyanin blueberry buccal tablets, raw material include: blueberry, microcrystalline cellulose, mannitol, citric acid and stearic acid
Magnesium.
Preparation method specifically includes the following steps:
1) blueberry is beaten: by Blueberry crushing and beating, after adding 1mg/g pectase, and homogenate, enzymolysis time is 1 ~
3h after the completion of enzymatic hydrolysis, obtains blueberry pulp, and the citric acid of blueberry pulp quality 1.5% is added；
2) be centrifugated: blueberry pulp is separated into blueberry juice and blueberry pomace by centrifuge, revolving speed 3000 ~
Under conditions of 5000r/min, it is centrifuged 10 ~ 20min；
3) preparation of blueberry ethanol solution: by blueberry juice according to 1:2(v/v) and dehydrated alcohol mixing, obtain blueberry ethyl alcohol
Solution；
4) by blueberry pomace according to solid-liquid ratio 1:20g/mL, pH=3.5, ethyl alcohol volume point blueberry pomace ultrasonic extraction: are added
Number is 55%(v/v) ethanol water, while under conditions of ultrasonic power 300-400W, 50 ~ 60 DEG C of Extracting temperature, ultrasound mentions
20-30 min is taken, Anthocyanin from Blueberry extracting solution is obtained；
5) it is centrifuged Anthocyanin from Blueberry extracting solution: by Anthocyanin from Blueberry extracting solution centrifugal treating, in revolving speed 3000r/min condition
Under, it is centrifuged 30min, filtrate is taken after filtering, obtains Anthocyanin from Blueberry extracting solution, filter residue is freeze-dried to obtain blueberry Polyose extraction
Object；
6) it is freeze-dried: Anthocyanin from Blueberry extracting solution being freeze-dried, Anthocyanin from Blueberry crude extract is obtained.
7) embed Anthocyanin from Blueberry crude extract: it is 3.0 mg/ that 15 mL concentration, which are added, in 10mg Anthocyanin from Blueberry crude extract
In mL carboxymethyl chitosan solution, under magnetic stirring apparatus effect, after reacting 15min, being slowly added to 30mL concentration dropwise is
1.2 mg/mL chitosan hydrochloride solution, under magnetic stirring apparatus effect, after reacting 1h, in 10,000 r/min condition of revolving speed
Under, it is centrifuged 10min, collects lower sediment.It is freeze-dried 12-16h, obtains stable Anthocyanin from Blueberry crude extract.
8) raw material mixes: taking stable Anthocyanin from Blueberry crude extract 5g, blueberry polyoses extract 20g, microcrystalline cellulose
15g, mannitol 15g, citric acid 2.5g, magnesium stearate 5g are sufficiently mixed, and 20mL blueberry ethanol solution is added, and blueberry is made
Lozenge softwood.
9) it is granulated and dry: blueberry buccal tablets softwood being put into oscillating granulator and is granulated, wet granular is placed in 30
Dry in DEG C air dry oven, 20min is stirred once, and the moisture content in particle is down to 3%, obtains blueberry particle.
10) it sterilizes: blueberry particle is irradiated into 10 ~ 40min sterilizing under ultraviolet light.
11) it tabletting: by blueberry particle tabletting in gnotobasis of sterilizing, and is packed in time, obtains high anthocyanin
Blueberry buccal tablets finished product.
After testing, lozenge 0.454g is suppressed, after testing, containing the anthocyanin of 0.33mg in lozenge, traditional blueberry contains
Anthocyanin content can not be detected in piece.
Embodiment 3
A kind of high anthocyanin blueberry buccal tablets, raw material include: blueberry, microcrystalline cellulose, mannitol, citric acid and stearic acid
Magnesium.
Preparation method specifically includes the following steps:
1) blueberry is beaten: by Blueberry crushing and beating, after adding 3mg/g pectase, and homogenate, enzymolysis time is 1 ~
3h after the completion of enzymatic hydrolysis, obtains blueberry pulp, and the citric acid of blueberry pulp quality 0.5% is added；
2) be centrifugated: blueberry pulp is separated into blueberry juice and blueberry pomace by centrifuge, revolving speed 3000 ~
Under conditions of 5000r/min, it is centrifuged 10 ~ 20min；
3) preparation of blueberry ethanol solution: by blueberry juice according to 1:2(v/v) and dehydrated alcohol mixing, obtain blueberry ethyl alcohol
Solution, extra blueberry juice filling can prepare blueberry juice product；
4) by blueberry pomace according to solid-liquid ratio 1:20g/mL, pH=2.5, ethyl alcohol volume point blueberry pomace ultrasonic extraction: are added
Number is 65%(v/v) ethanol water, while under conditions of ultrasonic power 300-400W, 50 ~ 60 DEG C of Extracting temperature, ultrasound mentions
20-30 min is taken, Anthocyanin from Blueberry extracting solution is obtained；
5) it is centrifuged Anthocyanin from Blueberry extracting solution: by Anthocyanin from Blueberry extracting solution centrifugal treating, in revolving speed 3000r/min condition
Under, it is centrifuged 30min, filtrate is taken after filtering, obtains Anthocyanin from Blueberry extracting solution, filter residue is freeze-dried to obtain blueberry Polyose extraction
Object；
6) it is freeze-dried: Anthocyanin from Blueberry extracting solution being freeze-dried, Anthocyanin from Blueberry crude extract is obtained.
7) embed Anthocyanin from Blueberry crude extract: it is 3.0 mg/mL carboxymethyl shells that concentration, which is added, in Anthocyanin from Blueberry crude extract
In glycan solution, under magnetic stirring apparatus effect, after reacting 15min, being slowly added to concentration dropwise is 1.2 mg/mL chitosans
HCI solution after reacting 1h, under the conditions of 10,000 r/min of revolving speed, is centrifuged 10min, receives under magnetic stirring apparatus effect
Collect lower sediment.It is freeze-dried 12-16h, obtains stable Anthocyanin from Blueberry crude extract.
8) raw material mixes: taking stable Anthocyanin from Blueberry crude extract 25g, blueberry polyoses extract 10g, microcrystalline cellulose
5g, mannitol 5g, citric acid 5g and magnesium stearate 10g, are sufficiently mixed, and 5mL blueberry ethanol solution is added, and blueberry is made and contains
Piece softwood.
9) it is granulated and dry: blueberry buccal tablets softwood being put into oscillating granulator and is granulated, wet granular is placed in 30
Dry in DEG C air dry oven, 20min is stirred once, and the moisture content in particle is down to 3%, obtains blueberry particle.
10) it sterilizes: blueberry particle is irradiated into 10 ~ 40min sterilizing under ultraviolet light.
11) it tabletting: by blueberry particle tabletting in gnotobasis of sterilizing, and is packed in time, obtains high anthocyanin
Blueberry buccal tablets finished product.
After testing, lozenge 0.462g is suppressed, after testing, containing the anthocyanin of 0.31mg in lozenge, traditional blueberry contains
Anthocyanin content can not be detected in piece.
Claims (4)
1. a kind of preparation method of stable high anthocyanin blueberry buccal tablets, which is characterized in that the preparation method specifically include with
Lower step:
Blueberry mashing: by Blueberry crushing and beating, after adding pectase, homogenate digests 1~3h, blueberry pulp is obtained, in indigo plant
Citric acid is added in certain kind of berries pulp；
Centrifuge separation: by blueberry pulp centrifugal treating, after centrifugation, blueberry pulp is separated into blueberry juice and blueberry pomace；
The preparation of blueberry ethanol solution: blueberry juice is mixed according to 1:2 (v/v) and dehydrated alcohol, obtains blueberry ethanol solution；
Ultrasonic extraction blueberry pomace: by blueberry pomace in the ethanol water of pH=2.5~3.5 ultrasonic extraction 20-30min,
Obtain Anthocyanin from Blueberry extracting solution；
Centrifugation Anthocyanin from Blueberry extracting solution: by Anthocyanin from Blueberry extracting solution centrifugal treating, filtrate is taken after filtering, obtains blueberry pattern
Glycosides extracting solution, filter residue is freeze-dried to obtain blueberry polyoses extract；
Freeze-drying: Anthocyanin from Blueberry extracting solution is freeze-dried, Anthocyanin from Blueberry crude extract is obtained；
Embedding Anthocyanin from Blueberry crude extract: Anthocyanin from Blueberry crude extract is added in carboxymethyl chitosan solution, is thoroughly mixed
Afterwards, chitosan hydrochloride solution is added, lower sediment is collected in centrifugation, is freeze-dried 12-16h, is obtained stable Anthocyanin from Blueberry
Crude extract；
Raw material mixing: stable Anthocyanin from Blueberry crude extract, blueberry polyoses extract, microcrystalline cellulose, mannitol, lemon are taken
Acid, magnesium stearate are sufficiently mixed, and blueberry ethanol solution is added, blueberry buccal tablets softwood is made；Wherein, stable Anthocyanin from Blueberry
5~25 parts of crude extract, 10~20 parts of blueberry polyoses extract, 5~20 parts of microcrystalline cellulose, 5~15 parts of mannitol, citric acid 2
~5 parts, 5~10 parts of magnesium stearate, 5~20 parts of blueberry ethanol solution；
It is granulated and dry: blueberry buccal tablets softwood being put into granulator and is granulated, and wet granular is set into drying process, obtains blueberry
Grain；
Sterilizing: blueberry particle is irradiated into 10~40min sterilizing under ultraviolet light；
Tabletting: by blueberry particle tabletting in gnotobasis of sterilizing, packaging, obtain stable high anthocyanin blueberry buccal tablets at
Product.
2. the preparation method of stable high anthocyanin blueberry buccal tablets according to claim 1, which is characterized in that the pectase
Additive amount be 0.1~3mg/g.
3. the preparation method of stable high anthocyanin blueberry buccal tablets according to claim 1, which is characterized in that the blueberries
The ultrasonic power of slag ultrasonic extraction are as follows: 300-400W, Extracting temperature are as follows: 50~60 DEG C.
4. the preparation method of stable high anthocyanin blueberry buccal tablets according to claim 1, which is characterized in that the carboxymethyl
The concentration of chitosan solution is 2.0-3.0mg/mL；The concentration of the chitosan hydrochloride solution is 0.6-1.2mg/mL.
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| CN106994142B (en) * | 2017-03-13 | 2021-01-29 | 安徽农业大学 | Loquat extract and loquat black tea extract buccal tablets capable of reducing blood sugar prepared from loquat extract |
| CN109077320A (en) * | 2018-10-12 | 2018-12-25 | 镇江市智农食品有限公司 | The chewable tablets and preparation method thereof of phenolic substances in a kind of absorption blueberry residue |
| CN110326785A (en) * | 2019-07-23 | 2019-10-15 | 大连工业大学 | Cationic marine polysaccharide-anthocyanin compound polyelectrolyte preparation method and applications in Caenorhabditis elegans service life can be extended |
| CN114479141B (en) * | 2022-01-27 | 2023-05-05 | 安徽农业大学 | Preparation and application of anthocyanin-rich semi-refined blueberry pectin edible film |
| CN115530370A (en) * | 2022-09-02 | 2022-12-30 | 沈阳农业大学 | Purple corn anthocyanin composite nutritional chewable tablet and preparation method thereof |
| CN115886255A (en) * | 2022-10-31 | 2023-04-04 | 中国农业大学 | High-stability blueberry anthocyanin binary gel tablet and preparation method thereof |
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