KR101298568B1 - Anti-obesity food composition containing extract of arctium lappa l - Google Patents
Anti-obesity food composition containing extract of arctium lappa l Download PDFInfo
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- KR101298568B1 KR101298568B1 KR1020130026684A KR20130026684A KR101298568B1 KR 101298568 B1 KR101298568 B1 KR 101298568B1 KR 1020130026684 A KR1020130026684 A KR 1020130026684A KR 20130026684 A KR20130026684 A KR 20130026684A KR 101298568 B1 KR101298568 B1 KR 101298568B1
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- extract
- burdock
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- food composition
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Images
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/30—Dietetic or nutritional methods, e.g. for losing weight
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/40—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by drying or kilning; Subsequent reconstitution
- A23L3/44—Freeze-drying
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
- A23V2200/332—Promoters of weight control and weight loss
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Abstract
Description
본 발명은 항비만용 식품조성물에 관한 기술로서, 특히, 우엉 추출물 등의 천연 물질을 포함하는 항비만 효과를 갖는 식품조성물에 관한 기술이다.
The present invention relates to a food composition for anti-obesity, and more particularly, to a food composition having an anti-obesity effect containing natural substances such as burdock extract.
본 발명은 우엉 추출물을 포함하는 체중감량 효과가 있는 항비만용 식품조성물에 관한 기술로서, 더욱 구체적으로는 우엉 추출물 이외에 가르시니아캄보지아껍질 추출물, 식이섬유, 비타민 C 및 황금 추출물을 포함하는 항비만용 식품조성물에 관한 기술이다.
The present invention relates to an anti-obesity food composition having a weight loss effect including a burdock extract, and more specifically, an anti-obesity food comprising garcinia cambogia bark extract, dietary fiber, vitamin C and golden extract in addition to burdock extract. It is a technique regarding a composition.
우엉(Arctium lappa L.)은 초롱꽃목 국화과의 두해살이 풀로, 일반적으로는 뿌리를 식용으로 하고, 어린 줄기잎도 먹는다. 야생종은 유럽, 시베리아, 만주 등지에 자생하며, 유럽에서는 간혹 어린 잎과 뿌리를 식용으로 하고, 중국에서는 종자를 우방자(牛蒡子)라 하여 이뇨제로 쓰고 있으나, 일본에서는 우엉이 채소로서 발달하였고, 우리나라에서도 채소로서의 용도로 그 재배가 점차 증가하고 있는 추세이다. 우엉의 열매에는 리그난 배당체인 아르커티인, 기름, 피토스테린, 비타민 B1, 사포닌, 쿠마린, 레몬산 등을 함유하고 있고, 뿌리에는 이눌린, 프로테인, 정유, 아르크테인, 카페인산, 탄닌, 알칼로이드 등이 함유되어 있는 것으로 알려져 있다. 우엉은 당질이 주성분으로 특유한 향기와 약리 효과가 있으며 섬유질은 많고 비타민은 적은 편이다. 하지만, 우엉은 다른 채소에 비하여 수분 함량이 적은 대신 당질의 함량이 높으며 그 대부분이 이눌린의 형태로 존재하므로 당뇨병 환자에게 매우 좋다고 알려져 있다. 또한, 우엉은 Trp-P-1 등 돌연변이원이 되는 여러 가지 아미노산 열분해 산물을 불활성화시키는 작용이 있으며, 이러한 작용은 우엉에 존재하는 열에 강한 리그닌(lignin) 유사물질로 알려져 있다.
Burdock (Arctium lappa L.) is a biennial herb of the Campanula Asteraceae. Its roots are usually edible and young stem leaves are also eaten. Wild species grow wild in Europe, Siberia, and Manchuria. In Europe, young leaves and roots are sometimes eaten, and in China, seeds are used as diuretics, but they are developed as burdock vegetables in Japan. In cultivation, the cultivation is gradually increasing as a vegetable. The fruit of burdock contains lignan glycosides, such as arcatine, oil, phytosterin, vitamin B1, saponin, coumarin, and lemon acid. It is known to contain. Burdock is a major component of sugar and has a distinctive scent and pharmacological effect. It is high in fiber and low in vitamins. However, burdock is known to be very good for diabetics because it has a lower sugar content than other vegetables and a high sugar content, most of which is in the form of inulin. In addition, burdock has a function of inactivating various amino acid pyrolysis products, such as Trp-P-1, which is known as a heat-resistant lignin analogue present in burdock.
가르시니아캄보지아는 구티페라(Guttifera)과 가르시니아(Garcinia) 종의 하나로 과피에는 HCA(Hydroxy Citric Acid)가 10 ~ 30% 함유되어 있다. HCA는 오렌지나 다른 밀감류에 존재하는 구연산과 매우 비슷한 물질로서, HCA를 가장 많이 함유하는 종은 가르시니아캄보지아로 알려져 있으며, 오랫동안 돼지고기 및 생선의 신맛을 내는 첨가물(souring agent)로서 남부 인디아 해안 지역에서 사용되어 왔다. 가르시니캄보지아는 다이어트를 할 때 섭취하면 큰 효과를 볼 수 있다고 알려져 있다.
Garcinia Cambogia is one of the Gutifera and Garcinia species, and the skin contains 10-30% of Hydroxy Citric Acid (HCA). HCA is a substance very similar to citric acid in oranges and other citrus fruits. The species that contains the most HCA is known as Garcinia cambogia, and has long been a souring agent for pork and fish. Has been used in. Garciny Cambogia is known to have a great effect on diet.
식이섬유(dietary fiber)란 신체의 소화효소로 분해되지 않는 난소화성 고분자 섬유 성분을 말하는데 주로 식물세포의 세포벽 또는 식물 종자의 껍질 부위에 분포되어 있다. 식이섬유는 신체에 흡수되지 않으므로 영양학적으로는 가치가 없는 것으로 인식되었다. 그러나 최근 기능성 식품에 대한 관심이 고조되어 탄수화물, 단백질, 지방, 비타민, 무기질 및 수분 등 6대 영양소와는 다른 생리기능을 인정하여 '제7의 영양소'라고 부르고 있다. 식이섬유의 생리적 기능성에는 변비, 치질, 대장암, 충수염 등의 예방에 효과적이며 고혈압, 동맥경화, 심장병 등의 순환기계 질환을 예방할 수 있다. 혈당치의 상승이 억제되어 당뇨병에도 좋으며, 비만 방지 효과도 있다. 식이섬유의 효능 중에서 일찍부터 주목되어 온 것은 변비의 예방 작용이다.
Dietary fiber refers to an indigestible polymer fiber component that is not degraded by the digestive enzymes of the body, and is mainly distributed in the cell wall of plant cells or the shell of plant seeds. Since dietary fiber is not absorbed by the body, it was perceived as nutritionally worthless. However, as interest in functional foods has recently increased, it is called 'the seventh nutrient' in recognition of physiological functions different from the six nutrients such as carbohydrates, proteins, fats, vitamins, minerals, and water. The physiological function of dietary fiber is effective in the prevention of constipation, hemorrhoids, colorectal cancer, appendicitis, and can prevent circulatory diseases such as hypertension, arteriosclerosis, and heart disease. The increase in blood sugar is suppressed and good for diabetes, and also has an anti-obesity effect. Among the benefits of dietary fiber has been noted early on is the prevention of constipation.
비타민 C는 6개의 탄소로 이루어진 락톤(lactone)이며 식물과 대부분의 동물은 체내에서 비타민 C를 합성할 수 있지만, 사람, 영장류, 기니아피그, 큰 박쥐, 몇 종의 조류와 어류는 합성할 수 없으므로 반드시 식품의 형태로 섭취해야 한다. 비타민 C라 함은 환원형의 아스코르빈산(ascorbic acid)과 산화형인 디하이드로아스코르빈산(dehydroascorbic acid)을 모두 포함하며, 이 두 물질은 상호 전환이 가능하다. 실제로 우리가 섭취하는 식품에는 2가지 형태가 모두 존재한다. 비타민 C는 생체 내에서 여러 가지 효소 반응의 조효소로 쓰이며, 피부, 골격, 연골 등의 결합조직을 구성하는 주요 단백질인 콜라겐의 합성에 관여한다. 또한, 비타민 C의 전자제공 기능은 활성산소종과 활성질소종 등의 자유기를 제거하는 항산화 활성을 띠게 한다. 또한, 비타민 C는 소장관에서 철분을 환원형으로 전환함으로써, 헤모글로빈에 결합되어 있지 않은 식물성 철분의 흡수를 촉진한다.
Vitamin C is a lactone of six carbons. Plants and most animals can synthesize vitamin C in the body, but humans, primates, guinea pigs, large bats, and some species of birds and fish can't. Must be taken in the form of food. Vitamin C contains both reduced ascorbic acid and oxidized dehydroascorbic acid, which can be converted to each other. In fact, there are two types of food we eat. Vitamin C is used as a coenzyme of various enzymatic reactions in vivo and is involved in the synthesis of collagen, a major protein constituting connective tissues such as skin, skeleton and cartilage. In addition, the electron donating function of vitamin C has an antioxidant activity that removes free radicals such as reactive oxygen species and active nitrogen species. Vitamin C also converts iron into the reduced form in the small intestine, thereby promoting the absorption of vegetable iron that is not bound to hemoglobin.
황금(Scutellaria baicalensis)은 꿀풀과에 속하는 다년생초로서, 전국 각지의 산 기슭에서 자라며 약초로도 재배되고 있다. 황금은 열을 내리고 해독 목적으로 자주 사용되는 약재로서 뿌리를 채취하여 뿌리 껍질을 벗겨보면 황금과 같이 노란색깔을 띄며, 몸에 열독이 오르거나 오한 등이 잦은 것을 치료하는데, 주로 폐에 열로 인한 증상에 다용(多用)되고, 열로 인한 갈증과 황달, 이질, 설사, 소화불량, 황련과 마찬가지로 몸에 있는 종기나 종양, 더욱더 심해져서 생기는 등창들을 치료한다. 특이하게 일반적으로 쓰이고 차가운 약은 임산부에게는 금지하여야 할 약재로 분류되는데, 황금의 경우 볶으면 임신중 태동불안 등으로 괴로움을 겪을 때에도 좋은 치료 효과가 있는 것으로 알려있다.
Golden (Scutellaria baicalensis) is a perennial herb belonging to the Lamiaceae family. It grows at the foot of mountains all over the country and is also grown as a herb. Gold is a medicinal herb that is frequently used for fever and detoxification. When the roots are removed and the skin is peeled off, the yellow color is golden like gold, and the body treats frequent heat poisoning or chills. It is used for the treatment of heat and thirst, jaundice, dysentery, diarrhea, indigestion, and dysentery in the body, such as fever, tumors, and even worse. Unusually commonly used and cold medicine is classified as a medicine that should be prohibited to pregnant women, if the golden fry is known to have a good therapeutic effect even when suffering from pregnancy anxiety.
한편, 우엉추출물을 이용한 종래 특허를 살펴보면, 특허등록 제10-874113호에서는 우엉 추출물을 유효 성분으로 하는 수렴 화장료 조성물을 공개하고 있으며, 특허등록 제10-767974호에서는 우엉추출물과 냉감성분을 함유하는 모공축소용 화장료 조성물을 공개하고 있으며, 특허공개 제10-2011-131850호는 우엉추출물을 함유한 항비듬 모발화장품 조성물에 대하여 공개하고 있으며, 특허공개 제10-2013-13950호는 우엉발효추출물을 포함하는 세제용 조성물을 공개하고 있다.
On the other hand, looking at the conventional patent using the burdock extract, Patent Registration No. 10-874113 discloses a convergent cosmetic composition containing the burdock extract as an active ingredient, Patent Registration No. 10-767974 contains a burdock extract and cold persimmon components A cosmetic composition for shrinking pores is disclosed, and Patent Publication No. 10-2011-131850 discloses an anti-dandruff hair cosmetic composition containing burdock extract, and Patent Publication No. 10-2013-13950 discloses a burdock fermentation extract. The composition for detergents containing is disclosed.
본 발명은 우엉추출물을 포함하는 항비만 효과를 갖는 식품조성물에 관한 기술을 제공하는 것으로서, 우엉추출물 이외에 기능 강화를 위하여 가르시니아캄보지아껍질 추출물, 식이섬유, 비타민 C 및 황금 추출물을 포함하는 기능성 건강식품용 조성물을 제공하는 것을 목적으로 한다.
The present invention provides a technique for a food composition having an anti-obesity effect including a burdock extract, for functional health foods including garcinia cambogia bark extract, dietary fiber, vitamin C and golden extract for enhancing function in addition to burdock extract It is an object to provide a composition.
또한, 본 발명은 우엉 향의 유지와 우엉 특유의 갈변이 되는 단점을 극복하고, 조성물을 액상 및 분말로 제조하여 저장성을 크게 향상시키는 것을 목적으로 한다.
In addition, the present invention aims to overcome the disadvantages of the maintenance of burdock flavor and browning peculiar to burdock, and to improve the shelf life by preparing the composition in liquid and powder.
상기의 목적을 달성하기 위하여, 본 발명은 50 ~ 95 중량% 우엉 추출물; 0.1 ~ 10 중량% 가르시니아캄보지아껍질 추출물; 0.1 ~ 30 중량% 식이섬유 ; 0.01 ~ 5 중량% 비타민 C; 및 0.01 ~ 5 중량%의 황금 추출물을 포함하여 이루어지는 항비만용 식품조성물을 제공한다.
In order to achieve the above object, the present invention is 50 to 95% by weight burdock extract; 0.1-10% by weight garcinia cambogia bark extract; 0.1 to 30% by weight dietary fiber; 0.01 to 5% vitamin C; And it provides an anti-obesity food composition comprising 0.01 ~ 5% by weight of golden extract.
특히, 상기 우엉 추출물은 액상 또는 분말 중 어느 하나의 제형을 사용할 수 있다.
In particular, the burdock extract may use any one of a liquid or powder formulation.
특히, 상기 우엉 추출물은 정제수, 주정 및 에탄올 중 어느 하나의 추출 용매를 사용하여 추출되는 것이 바람직하다.
In particular, the burdock extract is preferably extracted using any one of the extraction solvent of purified water, alcohol and ethanol.
특히, 상기 우엉 추출물 분말은 액상의 우엉 추출물을 농축하여 동결 건조하여 제조되는 것이 바람직하다.
In particular, the burdock extract powder is preferably prepared by freezing and drying the concentrated burdock extract.
특히, 상기 우엉 추출물은 용매를 이용한 추출 후에 분획용 용매를 사용하여 분획을 더 거친 분획물일 수 있다.
In particular, the burdock extract may be a fraction rougher fractionated using the solvent for fractionation after extraction with a solvent.
특히, 상기 분획에 사용되는 용매는 에탄올, 헥산, 클로로포름, 에틸아세테이트 및 부탄올에서 선택되는 1종 이상의 용매를 사용하여 분획할 수 있다.
In particular, the solvent used in the fraction may be fractionated using one or more solvents selected from ethanol, hexane, chloroform, ethyl acetate and butanol.
본 발명의 항비만용 식품조성물은 우엉 추출물 및 비타민 C를 통하여 항산화 효과를 얻고, 가르시니아캄보지아껍질 추출물 및 식이섬유를 통하여 체중감량 효과를 얻으며, 특히, 상기 비타민 C를 통하여 우엉 추출물의 갈변 현상을 방지하고, 황금 추출물을 통하여 전체 조성물의 보존성을 증가시키는 것을 특징으로 한다. The anti-obesity food composition of the present invention obtains an antioxidant effect through burdock extract and vitamin C, and gains weight loss through garcinia cambogia bark extract and dietary fiber, and particularly, prevents browning of burdock extract through vitamin C. And it is characterized by increasing the preservation of the entire composition through the golden extract.
본 발명의 항비만용 식품조성물은 항산화 실험을 통하여 우수한 항산화 효과를 확인할 수 있었고, 3T3-L1 지방전구세포를 통한 실험을 통하여 항비만 효과를 확인할 수 있었다.
The anti-obesity food composition of the present invention was able to confirm the excellent antioxidant effect through the antioxidant experiment, the anti-obesity effect through the experiment through the 3T3-L1 fat precursor cells.
도 1은 실험예 3의 결과로서, 3T3-L1 지방전구세포의 생존율에 미치는 영향에 대한 실험 결과이다.
도 2는 실험예 3의 결과로서, 3T3-L1 지방전구세포의 지방구(lipid droplet) 형성에 미치는 영향에 대한 실험 결과이다.
도 3은 실험예 3의 결과로서, 3T3-L1 지방전구세포의 중성지방 생성에 미치는 영향에 대한 실험 결과이다.1 is an experimental result of the effect on the survival rate of 3T3-L1 adipocytes as a result of Experimental Example 3.
Figure 2 is a result of Experiment 3, the experimental results of the effect on the formation of lipid droplet (lipid droplet) of 3T3-L1 adipocytes.
3 is an experimental result of the effect on the triglyceride production of 3T3-L1 adipocytes as a result of Experimental Example 3.
본 발명은 50 ~ 95 중량%의 우엉 추출물; 0.1 ~ 10 중량%의 가르시니아캄보지아껍질 추출물; 0.1 ~ 30 중량%의 식이섬유 ; 0.01 ~ 5 중량%의 비타민 C; 및 0.01 ~ 5 중량%의 황금 추출물을 포함하여 이루어지는 항산화 및 항비만 효과를 갖는 기능성 건강식품용 조성물을 제공한다.
The present invention 50% to 95% by weight of burdock extract; 0.1-10% by weight of garcinia cambogia bark extract; 0.1 to 30% by weight of dietary fiber; 0.01-5% by weight of vitamin C; And provides a functional health food composition having an antioxidant and anti-obesity effect comprising 0.01 to 5% by weight of the golden extract.
본 발명은 액상 또는 분말 형태의 우엉 추출물과 비타민 C를 이용하여 항산화 효과를 높이고, 가르시니아캄보지아껍질 추출물과 식이섬유를 이용하여 항비만 효과를 더 높이는 것을 특징으로 한다. 한편, 비타민 C는 항산화 효과를 높일 뿐만 아니라, 우엉 추출물의 갈변 현상을 방지하는 역할을 하는 것을 특징으로 한다. 또한, 황금 추출물은 자체적으로 항염, 항산화, 항알레르기 효과가 있다고 알려져 있으나, 본 발명에서는 본 조성물의 보존성 향상을 위한 목적으로 사용되었다.
The present invention is characterized by increasing the antioxidant effect by using a burdock extract and vitamin C in liquid or powder form, and using the garcinia cambogia bark extract and dietary fiber to further increase the anti-obesity effect. On the other hand, vitamin C is not only to increase the antioxidant effect, it is characterized in that it serves to prevent the browning phenomenon of burdock extract. In addition, the golden extract itself is known to have anti-inflammatory, antioxidant, anti-allergic effects, but in the present invention was used for the purpose of improving the preservation of the composition.
우엉 추출물Burdock Extract
우엉 추출물은 추출 용매로서 정제수, 주정 또는 에탄올을 이용한 추출한 액상 추출물 자체 또는 상기 액상 추출물을 동결 건조와 같이 분말화한 추출물을 모두 일컫는 포괄적 용어로 사용된다. 액상 추출물을 농축하여 동결건조 분말화 시킴으로 찬물에도 붕해도가 뛰어나 잘 녹고 기능성 물질을 90% 이상 함유하는 과립형 분말 제제로 이용하며 물에 녹였을 경우 우엉 고유의 향이 살아나도록 제조할 수 있다.
Burdock extract is used as a generic term that refers to both the liquid extract itself extracted with purified water, alcohol or ethanol as the extraction solvent, or the powdered extract such as freeze drying the liquid extract. The liquid extract is concentrated and lyophilized to make powder freeze, so it has excellent disintegration even in cold water, and is used as a granular powder formulation containing 90% or more of functional substances. When dissolved in water, the inherent aroma of burdock can be prepared.
본 발명에서 우엉 추출물은 액상 및/또는 분말 형태로서 50 내지 95 중량% 함유하는 것이 바람직하다. 상기 우엉 추출물은 액상 또는 분말 모두 가능하며, 액상의 경우 우엉 유효성분이 분말에 비하여 상대적으로 적게 된다. 또한, 우엉 추출물은 추출 용매를 통하여 추출한 후, 다시 다양한 용매, 예를 들어, 에탄올, 헥산, 클로라이드, 에틸아세테이트, 부탄올을 통하여 분획한 분획물을 사용할 수 있다.
Burdock extract in the present invention preferably contains 50 to 95% by weight in liquid and / or powder form. The burdock extract is available in both liquid and powder, and in the case of liquid, burdock active ingredient is relatively less than the powder. In addition, the burdock extract may be extracted through an extraction solvent, and then fractions may be used through various solvents such as ethanol, hexane, chloride, ethyl acetate, butanol.
가르시니아캄보지아껍질 추출물Garcinia Cambogia Bark Extract
가르시니아캄보지아껍질 추출물은 가르시나아캄보지아의 껍질을 통상의 추출 방법, 즉, 정제수나 알콜 등을 이용하여 유효 성분을 추출한 액상 추출물 또는 이를 분말화한 것을 모두 포함한다. 가르시니아캄보지아추출물은 전체 조성물 중 0.1 내지 10 중량% 사용되어 체중감량 효과를 준다.
Garcinia cambogia bark extract includes both the liquid extract or powdered extract of the active ingredient using a conventional extraction method, that is, purified water or alcohol, etc., of the bark of garcinia cambogia. Garcinia cambogia extract is used 0.1 to 10% by weight of the total composition to give a weight loss effect.
식이섬유Dietary Fiber
식이섬유는 0.1 ~ 30 중량% 식이섬유 사용되어, 체중 감량 효과를 증가시킨다.Dietary fiber is used 0.1 to 30% by weight of dietary fiber, increasing the weight loss effect.
비타민 CVitamin C
전술한 바와 같이, 종래에도 우엉을 주성분으로 하는 차 제품은 상품화되어 있으나, 기능성을 부각시킨 액상형 및 과립분말형의 제품은 출시가 전무하다. 이유는 우엉의 가공시 우엉 고유의 향취가 사라지고 우엉 자체의 갈변현상을 해결하지 못하였기 때문이다. 그러나 본 발명에서는 우엉의 갈변도 방지와 기능성 강화를 위하여 비타민 C를 더 첨가하는 것을 특징으로 한다. 비타민 C는 0.01 ~ 5 중량%로 상대적으로 소량 사용되어, 항산화 및 갈변방지 효과를 돕는데, 상기 함량보다 적으면 비타민 C의 효과가 전혀 없고, 너무 크면 조성물 가격이 올라가며 다른 성분의 함량이 상대적으로 적어져야 하기 때문이다.
As described above, tea products mainly containing burdock have been commercialized, but liquid and granular powder products that have enhanced functionality have never been released. The reason is that during the processing of burdock, the inherent flavor of burdock disappeared and the browning phenomenon of burdock itself could not be solved. In the present invention, however, vitamin C is further added to prevent browning of burdock and to enhance functionality. Vitamin C is used in a relatively small amount of 0.01 to 5% by weight, which helps to prevent antioxidant and browning. If it is less than the above amount, there is no effect of vitamin C. If it is too large, the price of the composition increases and the amount of other ingredients is relatively small. Because you have to lose.
황금 추출물Golden extract
황금 추출물은 정제수, 알콜 등을 이용한 통상의 추출 방법을 이용하여 추출하되, 함량은 0.01 ~ 5 중량%로 사용되어, 식품보존제로서의 역할을 수행한다. 식품보존제로 사용하기 때문에 다른 성분에 비하여 적은 양을 사용한다.
Golden extract is extracted using a conventional extraction method using purified water, alcohol, etc., the content is used in 0.01 to 5% by weight, serves as a food preservative. Because it is used as a food preservative, it is used in a small amount compared to other ingredients.
실시예Example
이하에서는 본 발명의 실시예 및 실험예를 통하여 본 발명의 효과에 대하여 설명하기로 한다.
Hereinafter, the effects of the present invention will be described through examples and experimental examples of the present invention.
실시예Example 1 : 본 발명의 조성물 제조(분말) 1: Preparation of the composition of the present invention (powder)
하기의 표 1과 같은 분말상의 조성물을 제조하였다.
To prepare a powdery composition as shown in Table 1 below.
실시예Example 2 : 본 발명의 조성물 제조(액상) 2: Preparation of the composition of the present invention (liquid)
하기의 표 2와 같은 액상의 조성물을 제조하였다.
To prepare a liquid composition as shown in Table 2 below.
실험예Experimental Example
이하에서는 상기 실시예에서 제조한 샘플을 대상으로 하기와 같은 항산화 및 항비만 효과에 대하여 실험하였다.
Hereinafter, the antioxidant and anti-obesity effects of the samples prepared in Examples were tested as follows.
실험예Experimental Example 1 : 총 폴리페놀 및 총 플라보노이드 함량 분석 1: Total Polyphenol and Flavonoid Content Analysis
상기 우엉 분말을 동결건조한 분말의 우엉 추출물을 포함하는 본 발명의 기능성 건강식품 조성물(실시예 1)과 우엉의 액상 추출물을 이용한 본 발명의 기능성 건강식품 조성물(실시예 2) 내에 존재하여, 항산화, 항염증, 항암 등의 유익성을 나타내며 인체 유용한 기능성 지표성분으로서 총칭되는 총 폴리페놀 및 총 플라보노이드를 측정하였다.
The functional health food composition of the present invention (Example 1) comprising the burdock extract of the powder of lyophilized powder of burdock powder and the functional health food composition of the present invention (Example 2) using a liquid extract of burdock, antioxidant, Total polyphenols and total flavonoids, collectively referred to as functional indicators useful for the human body, were shown to have beneficial effects such as anti-inflammatory and anti-cancer.
총 폴리페놀 함량은 Folin-ciocal법을 이용하여 측정하였으며, 지표물질로서 타닌산(Sigma, USA)을 사용하여 작성된 정량곡선을 통해 정량(mg/g)하였다. 총 플라보노이드 함량은 AlCl3법을 이용하여 측정하였으며, 지표물질로서 카테킨(Catechin, Sigma, USA)을 사용하여 작성되어진 정량곡선을 통해 정량화하였다.
The total polyphenol content was measured using the Folin-ciocal method and quantified by mg / g using a quantitative curve prepared using tannic acid (Sigma, USA) as an indicator. Total flavonoid content was measured using AlCl 3 method and quantified by quantitative curve prepared using catechin (Catechin, Sigma, USA) as an indicator.
총 폴리페놀 및 총 플로보노이드 측정 결과는 하기의 표 3과 같았다.
The total polyphenol and total flavonoid measurement results were shown in Table 3 below.
실시예 1의 조성물의 경우 총 폴리페놀 함량 측정 결과 322.90 mg/g, 총 플라보노이드 함량은 176.26 mg/g로 정량되었으며, 실시예 2의 조성물의 경우 총 폴리페놀 함량 측정 결과 141.08 mg/g, 총 플라보노이드 함량은 76.93 mg/g로 정량되었다.
For the composition of Example 1, the total polyphenol content was determined to be 322.90 mg / g, and the total flavonoid content was 176.26 mg / g. For the composition of Example 2, the total polyphenol content was determined to be 141.08 mg / g and the total flavonoids. The content was quantified at 76.93 mg / g.
실시예 1의 조성물은 우엉의 액상 추출물을 농축하여 분말화함으로써, 우엉의 유용 생리활성물질 함량이 실시예 2의 액상의 우엉 추출물에 비하여 높기 때문에, 동일 무게에서 총 폴리페놀과 총 플라보노이드 함량이 크게 나타났다.
The composition of Example 1 concentrated and powdered the liquid extract of burdock, so that the useful bioactive substance content of burdock was higher than that of the liquid burdock extract of Example 2, so that the total polyphenol and total flavonoid contents were large at the same weight. appear.
우엉 차는 음용시 적용되는 음용수의 온도 조건 및 추출 시간에 따라 섭취할 수 있는 생리활성량이 달라질 수 있으나, 실시예 1과 같은 분말 형상의 본 발명의 우엉 추출물을 포함하는 조성물은 음용시 작용하는 이런 환경 조건에 상관없이 매번 유사한 수준의 생리활성물질 섭취가 가능한 장점으로 인하여 상품 가치성이 높다. 실시예 1과 같이 분말 형태로 본 발명의 조성물을 이용하는 경우, 기존 우엉차, 우엉 추출물보다 적은 부피와 질량으로부터 목표하는 양의 생리활성물질의 섭취가 가능하다.
The amount of physiological activity that can be ingested may vary depending on the temperature conditions and extraction time of drinking water applied to drinking burdock tea, but the composition containing the burdock extract of the present invention in powder form as in Example 1 has such an environment in which it works upon drinking Regardless of the conditions, the merchandise value is high due to the advantage of being able to consume similar levels of bioactive substances each time. When using the composition of the present invention in powder form as in Example 1, it is possible to ingest the target amount of the bioactive material from the volume and mass less than conventional burdock tea, burdock extract.
실험예Experimental Example 2 : 항산화 검증 2: Antioxidant Verification
항산화 검증은 잔틴산화효소 활성 실험(Xanthine Oxidase activity assay) DPPH assay 및 SOD(Superoxide Dismutase) 실험을 통하여 확인하였다. 실험은 실시예 1의 조성물을 사용하여 평가하였다.Antioxidant verification was confirmed through Xanthine Oxidase activity assay DPPH assay and Superoxide Dismutase (SOD) experiment. Experiments were evaluated using the composition of Example 1.
(1) (One) 잔틴산화효소Xanthine oxidase 활성 실험( Active experiments ( XanthineXanthine OxidaseOxidase activityactivity assay)assay)
항산화효과를 알아보기 위하여 산화를 일으키는 효소인 잔틴산화효소(Xanthine oxidase) 활성실험을 통하여 확인하였다. 시료의 항산화효과를 비교하기 위하여 대조시료로서 아스코르빈산을 같은 농도로 측정하여 비교하였으며, 실험은 3회 반복하였다. 그 결과 표 4와 같았다.
In order to determine the antioxidant effect was confirmed through the activity test of xanthine oxidase, an enzyme that causes oxidation. In order to compare the antioxidant effect of the sample, ascorbic acid was measured and compared as a control sample, and the experiment was repeated three times. As a result, it was as Table 4.
잔틴산화효소 활성 분석(Xanthine Oxidase activity assay)을 통한 결과로서 본 발명의 조성물이 1 mg/ml의 농도 이상에서 97% 이상의 항산화 효과를 나타내었으며, 0.1 mg/ml에서 36%를 나타내었다. 반면에 대조시료인 아스코르빈산은 10 mg/ml에서 90% 수준의 항산화효과를 나타냈다. 본 발명의 조성물 1mg/ml에서의 항산화 효과가 대조군인 아스코르빈산의 10 mg/ml보다 높은 항산화 효과를 나타내어, 본 발명의 조성물의 항산화효과는 대조군인 아스코르빈산에 비하여 10배 정도의 항산화효과를 나타내는 것을 확인할 수 있었다.
As a result of Xanthine Oxidase activity assay, the composition of the present invention exhibited an antioxidant effect of more than 97% at a concentration of 1 mg / ml or more, and 36% at 0.1 mg / ml. In contrast, ascorbic acid, a control sample, showed an antioxidant effect of 90% at 10 mg / ml. The antioxidant effect of 1 mg / ml of the composition of the present invention exhibited an antioxidant effect higher than 10 mg / ml of ascorbic acid as a control group, and the antioxidant effect of the composition of the present invention was about 10 times higher than that of the control group of ascorbic acid. It could be confirmed that it represents.
(2) (2) DPPHDPPH assayassay
DPPH(1,1-diphenyl-2-picrylhydrazyl)는 화학적으로 안정화된 자유 라디칼을 가지고 있는 수용성 물질로 항산화 활성이 있는 물질과 만나면 전자를 내어주면서 라디칼이 소멸되고 색이 변한다. 이런 원리를 통해 DPPH로 먼저 산화를 개시한 후 시료에 의해서 라디칼에 대한 소거 활성 능력을 환산하여 항산화효과로 나타내었으며 실험은 3회 반복하였다. 그 결과는 표 5와 같았다.
DPPH (1,1-diphenyl-2-picrylhydrazyl) is a water-soluble substance with chemically stabilized free radicals. When it encounters an antioxidant-active substance, it gives off electrons and radicals disappear and change color. Through this principle, oxidation was first initiated with DPPH, and the scavenging activity of radicals by the sample was expressed as an antioxidant effect. The experiment was repeated three times. The results were shown in Table 5.
본 발명의 조성물
The composition of the present invention
Ascorbic acid
본 발명의 조성물에 대한 DPPH 항산화효과는 10 mg/ml에서 62%, 1 mg/ml에서 47%를 나타내었다. 대조군으로 진행된 아스코르빈산은 0.1 mg/ml에서 95% 이상을 나타내어 DPPH를 이용한 항산화실험에서 본 발명의 조성물을 이용한 시제품의 항산화효과는 아스코르빈산에 비해 10% 수준의 항산화 효과를 나타내는 것을 확인할 수 있었다. 물론 대조군인 아스코르빈산에 비하여 DPPH 항산화 효과는 낮았지만, 이러한 수치의 항산화 효과만으로도 매우 우수한 항산화 효과가 있다는 것을 확인할 수 있었다.
The DPPH antioxidant effect on the composition of the present invention was 62% at 10 mg / ml, 47% at 1 mg / ml. Ascorbic acid proceeded as a control group showed more than 95% at 0.1 mg / ml, and the antioxidant effect of the prototype using the composition of the present invention in the antioxidant experiment using DPPH showed an antioxidant effect of 10% compared to ascorbic acid. there was. Of course, the DPPH antioxidant effect was lower than that of the control group ascorbic acid, but it was confirmed that the antioxidant effect of these values alone was very excellent.
(3) (3) SODSOD (( SuperoxideSuperoxide DismutaseDismutase ) ) 유사활성Similar activity 시험 exam
SOD(Superoxide Dismutase) 유사활성 측정은 과산화수소(H2O2)로 전환시키는 반응을 촉매하는 피로갈롤(Pyrogallol)의 생성량을 측정하여 항산화효과로 환산하였다. 시료의 항산화효과를 비교하기 위하여 대조시료로서 아스코르빈산을 같은 농도로 측정하여 비교하였으며 실험은 3회 반복 실험하였다. 그 결과는 하기의 표 6과 같았다.
Superoxide Dismutase (SOD) -like activity was measured as an antioxidant effect by measuring the amount of pyrogallol that catalyzes the conversion of hydrogen peroxide (H 2 O 2 ). To compare the antioxidant effects of the samples, ascorbic acid was measured at the same concentration as a control sample, and the experiment was repeated three times. The results were as shown in Table 6 below.
The composition of the present invention
Ascorbic acid
우엉 조성물의 항산화효과는 10 mg/ml에서 91%, 1 mg/ml에서 57%를 나타내었다. 아스코르빈산의 결과와 비교해보면, 본 발명의 조성물 10 mg/ml은 아스코르빈산의 1 mg/ml과 유사한 수준의 항산화효과를 나타내었다. SOD 유사활성 측정을 통한 본 발명의 조성물의 항산화효과는 아스코르빈산에 비해 15 % 수준의 항산화효과를 나타내는 것을 확인할 수 있었다. 본 발명의 조성물에는 비타민 C가 0.07 중량%임을 감안하면 이러한 수준의 항산화 효과는 본 발명의 조성물이 매우 우수한 항산화 효과를 갖는 것을 의미한다.
The antioxidant effect of the burdock composition was 91% at 10 mg / ml and 57% at 1 mg / ml. Compared with the results of ascorbic acid, 10 mg / ml of the composition of the present invention showed an antioxidant effect similar to that of 1 mg / ml of ascorbic acid. The antioxidant effect of the composition of the present invention by measuring the SOD-like activity was confirmed to exhibit an antioxidant effect of 15% compared to ascorbic acid. Given that the composition of the present invention has a vitamin C of 0.07% by weight, this level of antioxidant effect means that the composition of the present invention has a very good antioxidant effect.
실험예Experimental Example 3 : 체중감량 검증 3: weight loss verification
본 발명의 조성물을 대상으로 체중감량 검증을 하였다. 3T3-L1 지방세포는 인비보(in vivo)에 존재하는 대사성 피드백루프에 관련되어 있는 복잡한 문제와 연류되지 않는 지방세포로 3T3-L1 전지방세포는 인슐린이나 그와 유사한 유도물질의 존재하에 지방세포로 분화하는 특성이 있고, 분화한 지방세포는 인슐린 수용체와 GLUT4(glutos transporter 4)를 세포막에 갖고 있어, 인슐린이 인슐린 수용체와 결합하여 신호전달과정을 통해 포도당의 섭취를 조절하는 역할을 하여, 인슐린의 작용력을 향상시키는 물질을 탐색하고 인슐린 선포전달 체계를 연구하는 모델로 많이 이용되고 있다.
Weight loss verification was performed on the composition of the present invention. 3T3-L1 adipocytes are adipocytes that are not related to the complex problems associated with in vivo metabolic feedback loops. 3T3-L1 adipocytes are adipocytes in the presence of insulin or similar inducers. Differentiation, and differentiated adipocytes have insulin receptor and GLUT4 (glutos transporter 4) on the cell membrane. Insulin binds to insulin receptor and regulates glucose intake through signaling process. It has been widely used as a model to search for substances that enhance the function of and to study insulin proclamation system.
특히, 본 발명에서는 각 조성물에서 우엉 추출물을 액상으로 추출한 후, 에탄올, 헥산, 클로로포름, 에틸아세테이트, 부탄올 및 물로 분획하여 실험하였다. 각 분획물은 분획 후 용매를 제거한 후, 동결건조하여 우엉 추출물을 분말화하여 본 발명의 조성물에 첨가하여 사용하였다.
In particular, in the present invention, after extracting the burdock extract from each composition in the liquid phase, the experiment was performed by fractionation with ethanol, hexane, chloroform, ethyl acetate, butanol and water. Each fraction was used after removing the solvent after fractionation, lyophilized to powder the burdock extract and added to the composition of the present invention .
각 우엉 분획물을 포함하는 본 발명의 조성물은 실시예 1과 동일한 함량으로 제조하여 이하의 실험에 사용하였다.
Compositions of the present invention containing each burdock fraction were prepared in the same amount as in Example 1 and used in the following experiments.
(1) 3(1) 3 T3T3 -- L1L1 지방전구세포의 생존율에 미치는 영향 Effect on the survival rate of fat progenitor cells
본 발명의 조성물의 3T3-L1 지방전구세포(preadipocytes)의 세포 생존율에 미치는 영향을 측정하기 위해, 20ug/ml의 농도로 각 시료를 처리한 후, MTT assay를 실시하였다. 그 결과는 도 1과 같았다. 참고로, 도 1의 x 축에서 O : oil red O염색약만을 처리한 대조군이며, 나머지는 각 우엉 분획물별로 본 발명의 조성물을 제조하여 실험한 것으로서, Et: 에탄올 분획물, H : 헥산 분획물, Ch : 클로로포름 분획물, EA : 에틸아세테이트 분획물, Bu :부탄올 분획물, W : 물분획물을 이용한 본 발명의 조성물을 의미한다.
In order to determine the effect on the cell viability of 3T3-L1 preadipocytes of the composition of the present invention, each sample was treated at a concentration of 20 ug / ml, followed by MTT assay. The results are shown in Fig. For reference, in the x-axis of FIG. 1 is a control group treated with O: oil red O dye only, the rest was tested by preparing the composition of the present invention for each burdock fraction, Et: ethanol fraction, H: hexane fraction, Ch: By chloroform fraction, EA: ethyl acetate fraction, Bu: butanol fraction, W: water fraction means a composition of the present invention.
시료 처리결과 우엉조성물을 처리하지 않은 대조군(O)의 생존율 100%로 보았을 때, 모든 본 발명의 조성물 20ug/ml의 농도에서 80% 이상의 생존율이 나타났다. 따라서 3T3-L1 지방전구세포의 생존에 영향을 끼치지 않으면서 세포독성이 없는 20ug/ml이하의 농도에서 이하 실험을 하였다.As a result of the sample treatment, the survival rate of 100% of the control group (O) not treated with the burdock composition showed a survival rate of 80% or more at the concentration of 20 ug / ml of all the compositions of the present invention. Therefore, the following experiment was conducted at concentrations of 20 ug / ml or less without cytotoxicity without affecting the survival of 3T3-L1 adipocytes.
(2) 3(2) 3 T3T3 -- L1L1 지방전구세포의 지방과립( Adipose granules of fat precursor cells lipidlipid dropletdroplet ) 생성에 미치는 영향) On the production
본 발명의 기능성 건강식품 조성물이 3T3-L1 전구지방세포의 지방구(lipid droplet) 형성에 미치는 영향 정도를 실험하였다. 지방전구세포를 분화 유도 후, 지방구(lipid droplet)만 특이적으로 염색하는 오일 레드 O(Oil red O) 염색시약을 이용하여 염색 후 현미경으로 관찰하였다. 본 발명의 각 조성물의 처리 농도는 10 ug/ml로 실험하였다.
The effect of the functional health food composition of the present invention on the formation of lipid droplets of 3T3-L1 progenitor cells was examined. After inducing differentiation, the progenitor cells were observed under a microscope after staining using an oil red O staining reagent that specifically stains only lipid droplets. Treatment concentrations of each composition of the invention were tested at 10 ug / ml.
그 결과 본 발명의 조성물을 처리하지 않고 분화를 유도하였을 경우, 세포질 내 지방구(Lipid droplet)의 형성이 활발하게 유발되는 것으로 관찰되고(O 샘플, 100% 기준), 본 발명의 각 조성물을 10ug/ml로 처리하였을 때 지방구형성이 억제되는 것을 관찰할 수 있었다. 그 결과는 첨부도 2와 같았다. 특히, 100% 이소프로필 알콜을 이용하여 염색된 지방구를 용출시켜 흡광도를 측정한 결과, 에틸아세테이트(EA)와 헥산(H)으로 분획한 우엉 추출물을 이용한 조성물에서 각각 22%와 19%의 감소율을 확인하여, 지방구 형성 억제효과가 가장 우수한 것을 확인할 수 있었다.
As a result, when differentiation was induced without treatment of the composition of the present invention, the formation of lipid droplets in the cytoplasm was actively induced (O sample, 100% basis), and each composition of the present invention was 10ug. When treated with / ml was observed to suppress the fat globules. The result was the same as in FIG. In particular, the absorbance was measured by eluting the stained fat spheres using 100% isopropyl alcohol, and the reduction rates of 22% and 19% in the composition using burdock extract fractionated with ethyl acetate (EA) and hexane (H), respectively. By confirming, it was confirmed that the fat globular formation inhibitory effect was the best.
(3) 지방전구세포 내 중성지방 생성에 미치는 영향(3) Effect on triglyceride production in adipose progenitor cells
중성지방(triglyceride)은 포도당과 함께 세포의 중요한 에너지원으로 사용되지만, 여분의 트리글리세리드는 지방세포에 흡수되고 저장되어 비만의 원인으로 작용한다.
Triglycerides are used together with glucose as an important energy source for cells, but extra triglycerides are absorbed and stored in fat cells and cause obesity.
3T3-L1 지방전구세포에 본 발명의 조성물을 처리한 결과 트리글리세리드 함량은 도 3과 같이 분화된 지방세포(O+ 샘플)와 비교했을 때, 모든 조성물에서 트리글리세리드 형성을 효과적으로 억제하는 것을 확인할 수 있었다. 특히, 에틸아세테이트로 분획한 우엉 추출물을 포함하는 본 발명의 조성물 10ug/ml의 농도를 처리하였을 때, 트리글리세리드 생성을 약 46% 저해하는 것으로 나타냈다. 이는 분화를 유도하지 않은 대조군(O- 샘플)과 유사한 트리글리세리드 함량을 보여, 매우 우수한 트리글리세리드 생성 억제 효과를 보였다.
As a result of treating the composition of the present invention to 3T3-L1 adipocytes, the triglyceride content was found to effectively inhibit triglyceride formation in all compositions as compared to the adipocytes differentiated as shown in FIG. In particular, when treated with a concentration of 10ug / ml of the composition of the present invention containing a burdock extract fractionated with ethyl acetate, it was shown to inhibit triglyceride production by about 46%. It showed a triglyceride content similar to that of the control group (O-sample) that did not induce differentiation, which showed a very good effect of inhibiting triglyceride production.
상기 결과와 같이 우엉 추출물을 다시 분획한 우엉 분획물의 경우 각 분획물 별로 체중 감량 효과에 차이는 있었으나, 전체적으로 모두 체중 감량 효과가 있음을 알 수 있었다. 따라서 체중 감량 효과를 위하여 에틸아세테이트로 분획하여 본 발명의 조성물에 사용하는 것도 바람직하지만, 경제성을 감안하여 분획 없이 단순 추출물 또는 상기 추출물의 분말을 그대로 사용하는 것도 가능함을 실험예 3을 통하여 확인할 수 있었다.
As shown in the above results, the burdock fraction, which was re-divided with the burdock extract, had a difference in weight loss effect for each fraction, but it was found that there was a weight loss effect as a whole. Therefore, although it is also preferable to use the composition of the present invention by fractionating with ethyl acetate for weight loss effect, it was confirmed through Experimental Example 3 that it is possible to use a simple extract or the powder of the extract as it is without fraction in view of economical efficiency. .
Claims (6)
0.1 ~ 10 중량% 가르시니아캄보지아껍질 추출물;
0.1 ~ 30 중량% 식이섬유 ;
0.01 ~ 5 중량% 비타민 C; 및
0.01 ~ 5 중량%의 황금 추출물을 포함하여 이루어지는 항비만용 식품조성물.
50-95 wt.% Burdock extract;
0.1-10% by weight garcinia cambogia bark extract;
0.1 to 30% by weight dietary fiber;
0.01 to 5% vitamin C; And
Anti-obesity food composition comprising 0.01 ~ 5% by weight of golden extract.
According to claim 1, wherein the burdock extract is an anti-obesity food composition, characterized in that the formulation of any one of liquid or powder.
According to claim 1, The burdock extract is an anti-obesity food composition, characterized in that the extraction using any one of the extraction solvent of purified water, alcohol and ethanol.
The anti-obesity food composition of claim 2, wherein the burdock extract powder is prepared by concentrated freeze-drying of a liquid burdock extract.
The method of claim 1, wherein the burdock extract is an anti-obesity food composition, characterized in that the fraction further coarse fraction using a solvent for fractionation after extraction with a solvent.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20160109762A (en) | 2015-03-13 | 2016-09-21 | 경일대학교산학협력단 | Method for preparing fermented extract of Arctium lappa L by using of mushroom strains |
| KR20180096023A (en) | 2017-02-20 | 2018-08-29 | 농업회사법인 주식회사 들산초 | Manufacturing method for fermentation vinegar of great burdock and its application to beverage |
| KR101896024B1 (en) | 2017-06-14 | 2018-09-06 | 주식회사 슈퍼내츄럴스 | Functional food compositions comprising extracts of Garcinia Cambogia as active ingredients and method for preparing the same |
| KR20180128582A (en) * | 2017-05-24 | 2018-12-04 | 농업회사법인 쌍계명차주식회사 | Food composition containing extract of roasted codonopsis lanceolata or roasted platycodon grandiflorum |
| CN111000226A (en) * | 2019-12-26 | 2020-04-14 | 甘肃大整健康管理股份有限公司 | A Chinese medicinal health preparation for dietetic therapy by balancing five ingredients for reducing weight |
| KR20210001194A (en) | 2019-06-27 | 2021-01-06 | 박호근 | Methods of manufacture of lactobacillus fermented burdock tea |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20040036112A (en) * | 2002-10-23 | 2004-04-30 | (주)바이오뉴트리젠 | A coffee composition comprising polyphenols, bioflavonoids or extracts of plants comprising polyphenols or bioflavonoids for improving the metabolism of the lipid and suppressing the fatness |
| KR20040040891A (en) * | 2002-11-08 | 2004-05-13 | (주)바이오뉴트리젠 | Carbohydrate foods comprising powders or extracts of plants comprising polyphenol or bioflavonoid for improving the metabolism of the lipid and suppressing the fatness |
-
2013
- 2013-03-13 KR KR1020130026684A patent/KR101298568B1/en active IP Right Grant
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20040036112A (en) * | 2002-10-23 | 2004-04-30 | (주)바이오뉴트리젠 | A coffee composition comprising polyphenols, bioflavonoids or extracts of plants comprising polyphenols or bioflavonoids for improving the metabolism of the lipid and suppressing the fatness |
| KR20040040891A (en) * | 2002-11-08 | 2004-05-13 | (주)바이오뉴트리젠 | Carbohydrate foods comprising powders or extracts of plants comprising polyphenol or bioflavonoid for improving the metabolism of the lipid and suppressing the fatness |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20160109762A (en) | 2015-03-13 | 2016-09-21 | 경일대학교산학협력단 | Method for preparing fermented extract of Arctium lappa L by using of mushroom strains |
| KR20180096023A (en) | 2017-02-20 | 2018-08-29 | 농업회사법인 주식회사 들산초 | Manufacturing method for fermentation vinegar of great burdock and its application to beverage |
| KR20180128582A (en) * | 2017-05-24 | 2018-12-04 | 농업회사법인 쌍계명차주식회사 | Food composition containing extract of roasted codonopsis lanceolata or roasted platycodon grandiflorum |
| KR102173223B1 (en) | 2017-05-24 | 2020-11-03 | 농업회사법인 쌍계명차주식회사 | Food composition containing extract of roasted codonopsis lanceolata or roasted platycodon grandiflorum |
| KR101896024B1 (en) | 2017-06-14 | 2018-09-06 | 주식회사 슈퍼내츄럴스 | Functional food compositions comprising extracts of Garcinia Cambogia as active ingredients and method for preparing the same |
| KR20210001194A (en) | 2019-06-27 | 2021-01-06 | 박호근 | Methods of manufacture of lactobacillus fermented burdock tea |
| CN111000226A (en) * | 2019-12-26 | 2020-04-14 | 甘肃大整健康管理股份有限公司 | A Chinese medicinal health preparation for dietetic therapy by balancing five ingredients for reducing weight |
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