CN107951915A - For improving cardiovascular raising immunity and antitumor Gynura procumbens (Lour.) Merr extract - Google Patents
For improving cardiovascular raising immunity and antitumor Gynura procumbens (Lour.) Merr extract Download PDFInfo
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- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
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- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
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Abstract
The present invention relates to for improving cardiovascular raising immunity and antitumor Gynura procumbens (Lour.) Merr extract.Specifically, relate in one aspect to prepare the method for the extract, including step：Gynura procumbens (Lour.) Merr dry powder is provided；Gynura procumbens (Lour.) Merr dry powder is soaked, is then decocted, leaching filtrate；Filter residue adds water to decoct again, leaching filtrate；Merge filtrate twice；It is concentrated into filtrate decompression；Add ethanol, stand, filtering；No alcohol taste is concentrated under reduced pressure into, it is dry, obtain Gynura procumbens (Lour.) Merr extract.Further relate to Gynura procumbens (Lour.) Merr extract, the composition comprising the extract, and the Gynura procumbens (Lour.) Merr extract as improve cardiovascular function, improve the immunity of the human body and/or antitumor product in purposes.Extract of the present invention can be used for improving cardiovascular and cerebrovascular symptoms, blood pressure lowering, hypoglycemic, reducing blood lipid, for toxin expelling, beauty, beauty treatment, anti-aging, and for improving function of immune system, improving immunity and having the function of suppression growth of tumour cell.
Description
Technical field
The invention belongs to medical and health product scope, is related to a kind of for improving cardiovascular and cerebrovascular symptoms, blood pressure lowering, drop blood
Sugar, reducing blood lipid, for toxin expelling, beauty, beauty treatment, anti-aging, and for improving function of immune system, raising immunity and having
There are the Gynura procumbens (Lour.) Merr extract for suppressing growth of tumour cell function, and the composition comprising the extract.The present invention also relates to
And the preparation method of described Gynura procumbens (Lour.) Merr extract and combinations thereof.
Background technology
Gynura procumbens (Lour.) Merr (Classification system：Gynura procumbens (Lour.)), people, which are also called, rescues heart dish, is chrysanthemum
The plant of section's Gynura Cass.It is distributed in Guangdong in Vietnam, Thailand, Indonesia and Africa and China, Hainan, Guizhou, cloud
The ground such as south, at present not yet by artificial introducing and planting.
Gynura procumbens (Lour.) Merr is that one kind climbs up by holding on to draft, there is foul smell, and stem is crawled, filbert or purple, has bar rib, when hairless or young
There is pubescence, there is branch；Leaf has handle；Blade is avette, ovate oval or ellipse, 3-8 centimetres long, 1.5-3.5 centimetres wide, top
Sharp or tapering, base portion round blunt or wedge-shaped narrow into petiole or has wave-formed mouth at full edge, and 5-7 pairs of lateral vein, arcuation is curved, thready pulse unobvious, on
Face green, below purple, two sides is hairless, dilute by thin pubescence；Petiole is 5-15 millimeters long, hairless, on top cauline leaf and inflorescence branch
Leaf is degenerated, lanceolar or wire lanceolar, stockless or nearly stockless.Basidixed or axillary corymb, each corymb tool 3-5
Capitulum；Peduncle is elongated, often there is the linear bracts of 1-3, by thin short thin hair or hairless.The narrow mitriform of involucre or funnel-form, long 15-
17 millimeters, 5-10 millimeters wide, base portion has the linear squamellas of 5-6；1 layer of phyllary, (9) 11-13, long round shape lanceolar, long 15-17
Millimeter, wide 1.5 milli come, and top is tapering, the narrow dry film matter in edge, has 1-3 bar middle arteries, does time-varying purple, hairless.Little Hua 20-30, orange
Yellow；Corolla is 12-15 millimeters long, and pipe portion is thin, 8-10 millimeters long, and top expands, sliver ovum shape lanceolar, top point；Flower pesticide base
Portion is blunt, and there is sharp triangle tag on top；Style branch taper, by mamillary microtriche.Achene cylinder, 4-6 millimeters long, chestnut is brown
Color, has 10 ribs, hairless；Pappus enriches, white, thin thin,tough silk hairy.
Gynura procumbens (Lour.) Merr be a kind of medicine food and can unique plant, the curled mallow that it and present people eat belongs to together.Food flavor
Soft and smooth, delicate fragrance is tasty.Can fry, is cold and dressed with sauce, float soup, cauline leaf chopping make dumpling, the filling of steamed stuffed bun has much fragrance, the taste of brew meal is outstanding
It is good.Its leaf is also raw-eaten, or takes fresh leaf boiling water to brew and work as tea-drinking, and it is even more not have some flavor that such as leaf, which is dried soaked,.
Gynura procumbens (Lour.) Merr young stem and leaf is full of nutrition, its protein, amino acid, total reducing sugar, fat content be respectively 1.24%,
0.87%th, 1.5%, 0.79%, dietary fiber content is 2.40 grams/100 grams, and Vitamin C content is 11.2 milligrams/100 grams, rich
It is the pollution-free food that middle-aged and elderly people replenishes the calcium containing organic calcium, also containing abundant Determination of Organic Acids and flavone compound.
Gynura procumbens (Lour.) Merr mainly eats young stem and leaf, has clearing heat and detoxicating, hemostasis cough-relieving, reduces blood vessel purpura, improves human body
Immunity and anti-virus ability；Purging intense heat, cool blood, anti-inflammatory, promote the production of body fluid and other effects, has the effect of certain for swollen disease.
Gynura procumbens (Lour.) Merr whole body is all precious, is studied through safety evaluatio, Gynura procumbens (Lour.) Merr is a kind of high-quality spy of nontoxic level
Kind health-care vegetable, rhizome is the excellent raw material of Chinese patent drug.It cooks edible or medicinal, can replenish the calcium, is antiviral, anti-flu,
Immunity and other effects is improved, external application has the effect of good to herpes zoster, various dermatitis, scald, burn and nameless gall, also
It can stop blooding, relieve pain, antipruritic and skin care moisturizing anti-aging.
According to《Chinese medicinal herb collects》Volume two is recorded：Its curative effect is slightly sweet flavor, and mild-natured, clearing and activating the channels and collaterals, inflammation-diminishing and cough-controlling, dissipates the stasis of blood
Detumescence, blood activating and promoting tissue regeneration.It is mainly used for treating traumatic injury, rheumatic arthritis and gout.
Through nutrition and chemical analysis, which is rich in organic calcium, is the optimal pollution-free food of replenishing the calcium of middle-aged and elderly people；Lie down chrysanthemum
Pseudo-ginseng contains abundant Determination of Organic Acids and flavone compound, in Determination of Organic Acids neochlorogenic acid have it is antiviral well and
Strengthen immunity, have obvious action to suppressing hepatitis type B virus, also with AIDS virus resisting and influenza
The effect of virus.
At present, existing many documents are had been reported that on the various technical research of Gynura procumbens (Lour.) Merr.
In terms of the extraction process of Gynura procumbens (Lour.) Merr.(Ye Zhi is red etc., ultrasonic wave extraction Gynura procumbens (Lour.) Merr total triterpene by Ye Zhihong etc.
Technical study, food industry, 02 phase in 2016) use ultrasonic wave extraction Gynura procumbens (Lour.) Merr total triterpene, investigate volume fraction of ethanol,
This influence of four factors to triterpene yield of ultrasonic power, ultrasonic time and solid-liquid ratio, it is true by single factor experiment and orthogonal test
The optimum process condition of extraction is determined.The result shows that volume fraction of ethanol 85%, ultrasonic time 40min, ultrasonic power 126W,
Extraction effect is optimal during 1 ︰ 30 (g/mL) of solid-liquid ratio, and triterpene yield is up to 1.3271%.
(Ye Zhi is red etc., and response phase method optimizes the technical study of microwave radiation exaraction Gynura procumbens (Lour.) Merr triterpene, food by Ye Zhihong etc.
Product industrial technology, 02 phase in 2016) it have studied technique with microwave radiation exaraction Gynura procumbens (Lour.) Merr triterpene.Investigation concentration of alcohol,
Influence of the factors such as microwave time, microwave power, Extracting temperature and solid-liquid ratio to Gynura procumbens (Lour.) Merr triterpene extraction yield.In Dan Yin
Box-Behnken experimental design methods and Responds Surface Methodology are utilized on plain experiment basis, determines its optimal extract process.
Test result indicates that when concentration of alcohol 90% and microwave power 600W, microwave radiation exaraction Gynura procumbens (Lour.) Merr triterpene it is optimal
Extraction conditions are：Microwave time 8.6min, 57.7 DEG C of Extracting temperature, solid-liquid ratio 1: 33g/mL.Gynura procumbens (Lour.) Merr on this condition
The recovery rate of triterpene has good fitness between 2.13%, with model predication value 2.14%.
(Zheng Guodong etc., the technical study of general flavone, the Jiangsu's agriculture section in microwave radiation exaraction Gynura procumbens (Lour.) Merr such as Zheng Guodong
Learn, 08 phase in 2015) use microwave radiation exaraction Gynura procumbens (Lour.) Merr in general flavone, investigate concentration of alcohol, solid-liquid ratio, microwave power,
Influence of 4 factors of microwave time to general flavone recovery rate, the optimal work of extraction is determined by single factor experiment and orthogonal test
Skill condition.L9 (34) orthogonal experiments show, influence size order of each factor to general flavone recovery rate in Gynura procumbens (Lour.) Merr
For concentration of alcohol>Microwave power>Solid-liquid ratio>The microwave time；The optimised process of general flavone extraction is microwave power 700W, solid-liquid ratio
1g: 25mL, microwave time 20min, concentration of alcohol 60%, the yield of general flavone is 2.208%.
(Li Jingen etc., the extraction process of optimization of orthogonal test Gynura procumbens (Lour.) Merr polysaccharide, food research is with opening by Li Jingen etc.
Hair, 05 phase in 2017) polysaccharide component distilled in flooding Gynura procumbens (Lour.) Merr is used, respectively with Extracting temperature, extraction time, feed liquid
Than being major influence factors with extraction time, using L9(34) Orthogonal Experiment and Design carry out extraction process optimization.It is the result shows that each
Factor is followed successively by Extracting temperature to the influence degree of Gynura procumbens (Lour.) Merr polysaccharide yield>Solid-liquid ratio>Extraction time>Extraction time.It is logical
Cross orthogonal test and its checking test determine that its optimum extraction process is 100 DEG C, extraction time 3h of Extracting temperature, solid-liquid ratio 4:
100 (g/mL), extraction time 2 times.On this condition, Gynura procumbens (Lour.) Merr polysaccharide yield is up to 19.88%.
(Wei Jing etc., response phase method Optimization for Ultrasonic Wave extraction Gynura procumbens (Lour.) Merr polyphenol technique, food research is with opening by Wei Jing etc.
Hair, 19 phases in 2017) using Gynura procumbens (Lour.) Merr powder as raw material, on the basis of single factor test, selection concentration of alcohol, ultrasonic temperature,
Ultrasonic time, ultrasonic power are independent variable, and Gynura procumbens (Lour.) Merr polyphenol recovery rate is response, according to center combination (Box-
Benhnken) experimental design principle, simulates to obtain quadratic polynomial recurrence side using the horizontal Responds Surface Methodology of four factor three
The prediction model of journey, optimizes Gynura procumbens (Lour.) Merr polyphenol ultrasonic extraction process.Gained optimum extraction process parameter is：Concentration of alcohol
55%, 70 DEG C, ultrasonic time 53mim, ultrasonic power 106W of ultrasonic temperature, on this condition, Gynura procumbens (Lour.) Merr polyphenol recovery rate
It is basically identical with predicted value 0.524% for 0.522%.
The chemical composition of Gynura procumbens (Lour.) Merr and quality control aspect.(Gong Shengshuai etc., the chemistry of Gynura procumbens (Lour.) Merr such as Gong Shengshuai
Composition Study (I), Chinese herbal medicine, 11 phases in 2016) research Gynura procumbens (Lour.) Merr Gynura procumbens herbs chemical composition.
Utilize silica gel column chromatography repeatedly, Sephadex LH-20 gel column chromatographies, middle compression leg chromatography and half preparative high-performance liquid chromatographic etc.
Method isolates and purifies, and passes through the authenticating compound structure such as NMR, MS.As a result from 70% ethanol extract of Gynura procumbens (Lour.) Merr herb
In isolated 16 compounds, be identified respectively as Quercetin (1), apiolin (2), cyanidenon (3), Kaempferol (4), radix astragali
Glycosides (5), Kaempferol -5-O- (6 "-O- acetyl group)-β-D- glucopyranosides (6), negletein (7), 4- methoxyl group meat
Cinnamic acid (8), benzyl glucoside (9), 2- phenethyl-O- β-D- glucopyranosides (10), 3,5- two-caffeoyl quinic acid
Methyl esters (11), 3,5- two-caffeoyl quinic acid ethyl ester (12), 3,4- two-caffeoyl quinic acid methyl esters (13), 4,5-, bis- coffees
Acyl group quininic acid methyl esters (14), protocatechuic acid (15) and syringin (16).Wherein, compound 7,8,12,14 and 16 is first
Isolated from Gynura Cass plant, compound 3,6,9~11 and 13 is isolated from the plant first.
Grain husk wait (grain husk etc., the separation and identification of Gynura procumbens (Lour.) Merr study on chemical compositions of leaves, Shenyang Pharmaceutical University's journal, 2012
Year 05 phase) research Gynura procumbens (Lour.) Merr leaf chemical composition.Using silica gel column chromatography, anti-phase ODS column chromatographies, Seph-
Adex LH-20 column chromatographies and HPLC methods etc. are isolated and purified, and pass through its chemical constitution of Spectrum Analysis technical appraisement.Knot
Fruit shows that isolated 7 compounds, are identified respectively as cupreol -3-O- O-gentibiosides from Gynura procumbens (Lour.) Merr leaf
(β-sitosterol-3-O-gentiobioside, 1), 3- indolecarboxylic acids (indole-3-carboxylic acid, 2), grasshopper
Grasshopper ketone (grasshopper ketone, 3), cupreol (β-sitosterol, 4), ordeal bean ketone (drummondone A,
5), n-dotriacontanol (n-dotriacontanol, 6) and ordeal bean ethyl ketone (drummondone B, 7).Wherein compound 1~
3rd, 5,7 be isolated from Gynura Cass plant first, and compound 4,6 is isolated from Gynura procumbens (Lour.) Merr first.
You Jiaojiao etc. (it is outstanding tender tender etc., HPLC-MS/MS methods measure at the same time in Gynura procumbens (Lour.) Merr water extract 9 kinds it is main effectively into
Point, Chinese herbal medicine, 02 phase in 2017) establish a kind of suitable while measure Gynura procumbens (Lour.) Merr Gynura procumbens water extracts
In 9 kinds of principle active components (apiolin, Quercetin, rutin, myricetin, chlorogenic acid, protocatechuic acid, p-Coumaric Acid, caffeic acid,
Kaempferol) HPLC-MS/MS methods.Its method is that 40mg Gynura procumbens (Lour.) Merrs water extract is through methanol (each 4mL) ultrasonic extraction 2
It is secondary, merge extracting solution and constant volume to 10mL.Chromatographic isolation is using Shim-pack ODS C18 (150mm × 2.0mm, 4.6 μm) points
From column, the aqueous formic acid of methanol -0.1% is mobile phase, gradient elution, volume flow 0.2mL/min.Mass Spectrometer Method using bear from
Sub- multiple-reaction monitoring pattern, and using honokiol as internal standard.The results show that it is dense in quality measurement to survey 9 kinds of principle active components
Linear relationship is good in the range of degree, and r is all higher than 0.9995；Precision, repeatability and have good stability；Mean sample recovery rate is
91.0%~101.3%, RSD≤3.31%.Thus the HPLC-MS/MS methods established are used for while measure Gynura procumbens (Lour.) Merr water
9 kinds of principle active components in extract, high sensitivity, specificity are good.
Zheng little Hua etc. (Zheng little Hua etc., HPLC method measure Gynura procumbens (Lour.) Merr Content of Chlorogenic Acid, Jiangxi traditional Chinese medicine, 2017
03 phase) establish the HPLC assay methods of Gynura procumbens (Lour.) Merr Content of Chlorogenic Acid.This method using YMC-Pack ODS-A (250mm ×
4.6mm, 5 μm) chromatographic column；With the phosphoric acid water of acetonitrile -0.1% (12: 88) for mobile phase, flow velocity 1mL/min, column temperature is 30 DEG C,
Detection wavelength is 327nm.The result shows that chlorogenic acid has good linear relationship (r=in 0.08064~1.008 μ g ranges
0.9996), mean sample recovery rate 100.92%, RSD 3.2%.This method is easy, accurate, reproducible, can be used as flat
The method of quality control of sleeping chrysanthemum pseudo-ginseng.
In terms of biological activity in relation to Gynura procumbens (Lour.) Merr.(Zheng Guodong etc., Gynura procumbens (Lour.) Merr is to mouse blood sugar by Zheng Guodong etc.
And the influence of blood fat, modern food science and technology, 12 phases in 2013) hyperglycaemia and hyperlipidemia serious threat human health are directed to, its
Influence of the research and inquirement Gynura procumbens (Lour.) Merr (GPM) to mouse blood sugar and blood fat, and its effect is illustrated by molecular biology method
Mechanism.40 kunming mices are randomly divided into 4 groups, i.e. control group, 2%, 4% and 8%GPM, feed mouse 12 weeks, weigh mouse
Weight and internal organs, measure lipid content in the biochemical indicator and liver and excrement in blood, with the methods of qRT-PCR and western blot points
Analyse the change of liver mRNA and protein expression.Mouse weight is increased the results show GPM and organ weights do not have significant change.
Triglycerides (TG), T-CHOL, the courage of low-density lipoprotein that more than 4% GPM is significantly reduced compared with control group in blood are consolidated
The concentration of TG in alcohol and blood glucose and liver.And the TG in excrement substantially increases.8%GPM substantially suppresses hydroxymethyl glutaryl CoA also
The vigor and mRNA and protein expression level of protoenzyme (HMGCR), and the mRNA of Glut4 and protein expression substantially increase.Tie above
Fruit shows GPM by adjusting the mRNA and protein expression of HMGCR and Glut4, and suppresses enteron aisle and TG is absorbed, and reaches hypoglycemic
Effect.
Zheng Guodong etc. (Zheng Guodong etc., Gynura procumbens (Lour.) Merr extraction and the research of antibacterial action, food science and technology, 04 phase in 2014)
Gynura procumbens (Lour.) Merr is extracted with 70% ethanol, is extracted respectively with petroleum ether, chloroform, ethyl acetate after concentration.Water phase (raffinate
Phase) cross HPD600 macroreticular resins after, separated with HPLC.Isolated difference extract carries out the antibacterial of 3 kinds of bacteriums and 2 kinds of fungies
Experiment.The result shows that each extract is not notable to fungi restraining effect, but ethyl acetate and water have obvious suppression to make with respect to bacterium
With wherein water phase effect is best, and the antibacterial circle diameter to staphylococcus aureus is 22.9mm.Separated 4 kinds of components in water phase
There is preferable fungistatic effect, wherein F1 reaches 24.7mm to the antibacterial circle diameter of staphylococcus aureus.
(Mu Qumei etc., influence of the Gynura procumbens (Lour.) Merr to diethylnitrosamine inducing mouse hepar damnification, the China such as Mu Qumei
Hospital pharmacy magazine, 08 phase in 2016) inquiring into Gynura procumbens (Lour.) Merr stem extraction (Ethanol extraction from
Gynura Procumbens stem, EEGS) to diethylnitrosamine (Diethylnirtosamine, DEN) inducing mouse
In the protective effect of the property learned hepatic injury, 40 Kunming male mices are randomly divided into 5 groups (every group 8)：Blank control group, give
Medicine control group, DEN model groups, EEGS treatment groups (50mgkg-1 and 200mgkg-1), model and treatment group orally give weekly
165mgkg-1DEN is given, stops DEN processing after 8 weeks, after Cheng Mo, treatment group and the daily gavage corresponding dosage of control group is administered
EEGS, put to death mouse after 7d.Serum is used to detect ALT, AST activity；HE, TUNEL and PAS dyeing detect histopathology respectively
, Apoptosis and glycogen levels；The changes in gene expression such as RT-PCR detection livers TNF-α, AP-2.The results show and model group phase
Than EEGS (200mgkg-1) can effectively reduce the Serum ALT of DEN inductions, AST activity and TNF-α mRNA and AP-2mRNA
Rise, and the reduction of the rise and IL-6mRNA to PPAR- γ, HGF mRNA is without obvious effect；And it can effectively reduce apoptosis
Cell quantity simultaneously suppresses glycogen and increases, hence it is evident that improves the pathological changes such as reduction and the steatosis of hepatic parenchymal cells.
In addition, Meng Xing etc. (Meng Xing etc., Gynura procumbens (Lour.) Merr extract Study on Its Antioxidant Activity in Vitro, food and machinery, 2016
10 phases of year) Gynura procumbens (Lour.) Merr plant different parts, drying mode and Extraction solvent are have studied to biological active matter in its extracting solution
The influence of matter content and antioxidation activity.The result shows that：Gynura procumbens (Lour.) Merr plant root is compared to stem and leaf portion, phenols chemical combination
Thing and flavonoid content is higher and inoxidizability is stronger, iron ion reduction/oxidation resistance, DPPH radical scavenging activities
The anti-oxidant value (oxidation resistance TEAC values) of power and ABTS~+Scavenging activity measure respectively reaches (21.25 ± 0.31),
(35.27 ± 0.26), (56.20 ± 0.22) μ g Trolox/mL.Drying mode is to Gynura procumbens (Lour.) Merr plant root extracting solution
Bioactive substance content has a significant impact, and the extracting solution that freeze-drying and 60 DEG C of heated-air drying modes obtain has stronger
Antioxidation activity.Solvent has significantly the bioactive substance content and antioxidation activity of Gynura procumbens (Lour.) Merr plant root extracting solution
Property influence (P<0.05), its antioxidation activity (FRAP methods, DPPH methods and ABTS methods) is shown with total phenol, general flavone and chlorogenic acid content
Write correlation (P<0.05) it is respectively, 0.919,0.848,0.907 with total phenol coefficient R, with general flavone content coefficient R point
Not Wei 0.915,0.793,0.823, be respectively 0.887,0.927,0.900 with chlorogenic acid content coefficient R, with VC content phases
Closing property is relatively low.
It is well known that biological activity of the recovery rate of chemical composition usually with the extract is in just in plant extracts
Correlation, to obtain the extraction of main typical active ingredient apiolin, Quercetin, rutin, myricetin, chlorogenic acid extracted amount characterization
Thing, for obtaining the Gynura procumbens (Lour.) Merr extract with more efficient biological effect with important opinion.
However, the prior art is relatively deficient in terms of the research of Gynura procumbens (Lour.) Merr extract, Gynura procumbens (Lour.) Merr extraction is improved
Method is still that those skilled in the art compel to obtain the Gynura procumbens (Lour.) Merr extract for including more main typical active ingredients
Cut expectation.
The content of the invention
It is an object of the invention to provide a kind of method for preparing Gynura procumbens (Lour.) Merr extract, expects flat obtained by this method
It is such as, but not limited to celery comprising more main typical active ingredients, these typical active ingredients in sleeping chrysanthemum Notogineng Extract
Element, Quercetin, rutin, myricetin, chlorogenic acid.It has been had now surprisingly been found that, the present invention prepares Gynura procumbens (Lour.) Merr extract
Method can be beneficial realize that above-mentioned technology it is expected.The present invention is consequently found that and be accomplished.
For this reason, first aspect present invention provides the method for preparing Gynura procumbens (Lour.) Merr extract, it includes the following steps：
(1) provide through the dry Gynura procumbens (Lour.) Merr to moisture less than 10%, be ground into most coarse powder, obtain Gynura procumbens (Lour.) Merr
Dry powder；
(2) make Gynura procumbens (Lour.) Merr dry powder add 5~8 times amount water immersion 1~3 it is small when, then decoct 30~120 minutes, filter
Take filtrate；Filter residue adds the water of 3~6 times of amounts to decoct again 30~120 minutes, leaching filtrate；Merge filtrate twice；
(3) white bole and propane diols are added into filtrate, is stirred evenly, when standing 2~4 is small, is depressurized at 50~70 DEG C
It is concentrated into 1/5 to 1/3 volume；Ethanol is added to stir evenly to alcohol content up to 50~60%, when standing 2~4 is small, filter to remove
Solid content；
(4) be concentrated under reduced pressure into no alcohol taste at 50~70 DEG C, then it is spray-dried be less than 5% to moisture, put down
Sleeping chrysanthemum Notogineng Extract.
In method according to a first aspect of the present invention, wherein step (1), the drying is drying mode chosen from the followings：
It is dried up or heat drying.
In method according to a first aspect of the present invention, wherein step (1), the drying is that drying to moisture is less than
8%.
In method according to a first aspect of the present invention, wherein step (2), for the first time plus 5~6 times of water measured soak.
In method according to a first aspect of the present invention, wherein step (2), for the first time plus 5~6 times of water immersions 1~2 measured are small
When.
In method according to a first aspect of the present invention, wherein step (2), for the first time plus 5~6 times of water immersions 1~2 measured are small
When, then decoct 30~60 minutes.
In method according to a first aspect of the present invention, wherein step (2), filter residue adds the water of 3~4 times of amounts to decoct again.
In method according to a first aspect of the present invention, wherein step (2), filter residue adds the water of 3~4 times of amounts to decoct 30~60 again
Minute.
In method according to a first aspect of the present invention, wherein step (3), the amount of white bole is the 0.5~1% of amount of filtrate
(g/100ml)。
In method according to a first aspect of the present invention, wherein step (3), the amount of white bole for amount of filtrate 0.5~
0.75% (g/100ml).
In method according to a first aspect of the present invention, wherein step (3), propane diols is 1,2-PD.
In method according to a first aspect of the present invention, wherein step (3), the amount of propane diols is the 0.2~0.6% of amount of filtrate
(g/100ml)。
In method according to a first aspect of the present invention, wherein step (3), the amount of propane diols is the 0.2~0.4% of amount of filtrate
(g/100ml)。
In method according to a first aspect of the present invention, wherein step (3), white bole and propane diols, stirring are added in filtrate
Uniformly, when standing 2~3 is small.
In method according to a first aspect of the present invention, wherein step (3), white bole and propane diols, stirring are added in filtrate
Uniformly, when standing 2~3 is small, it is concentrated under reduced pressure at 50~60 DEG C.
In method according to a first aspect of the present invention, wherein step (3), white bole and propane diols, stirring are added in filtrate
Uniformly, when standing 2~3 is small, 1/5 to 1/4 volume is concentrated under reduced pressure at 50~60 DEG C.
In method according to a first aspect of the present invention, wherein step (3), add ethanol to alcohol content up to 50~55%.
In method according to a first aspect of the present invention, wherein step (3), ethanol is added to be stirred to alcohol content up to 50~55%
Uniformly, when standing 2~3 is small, filter to remove solid content.
In method according to a first aspect of the present invention, wherein step (4), no alcohol taste is concentrated under reduced pressure at 55~65 DEG C.
In method according to a first aspect of the present invention, wherein step (4), no alcohol taste is concentrated under reduced pressure at 55~65 DEG C,
It is spray-dried again to be less than 4% to moisture, obtain Gynura procumbens (Lour.) Merr extract.
Method according to a first aspect of the present invention, apiolin, quercitrin are included in the Gynura procumbens (Lour.) Merr extract obtained by it
Element, rutin, myricetin, chlorogenic acid.
Method according to a first aspect of the present invention, apiolin, Quercetin, reed in the Gynura procumbens (Lour.) Merr extract obtained by it
Fourth, myricetin, chlorogenic acid overall recovery are more than 50%.Term " rate of recovery " refers to the prostrate chrysanthemum obtained by the method for the present invention
Five kinds of chemical substance total amounts account for that to prepare five kinds of chemical substances in Gynura procumbens (Lour.) Merr dry powder used in the extract total in Notogineng Extract
The percentage of amount, it reflects that five kinds of chemical substances reach the recycling of extract whole process of the present invention from dry powder through the method for the present invention
Rate, this rate of recovery is higher to show that the method for the present invention is higher for the efficiency for putting forward this five kinds of chemical substances.
Method according to a first aspect of the present invention, apiolin, Quercetin, reed in the Gynura procumbens (Lour.) Merr extract obtained by it
Fourth, myricetin, chlorogenic acid overall recovery are more than 60%.
Method according to a first aspect of the present invention, apiolin, Quercetin, reed in the Gynura procumbens (Lour.) Merr extract obtained by it
Fourth, myricetin, chlorogenic acid overall recovery are more than 70%；Such as five overall recovery can reach 70~85% scope it is special
Be can reach 75~85% scope.
Method according to a first aspect of the present invention, apiolin, Quercetin, reed in the Gynura procumbens (Lour.) Merr extract obtained by it
Fourth, myricetin, chlorogenic acid overall recovery are more than 75%.
Method according to a first aspect of the present invention, apiolin, quercitrin are included in the Gynura procumbens (Lour.) Merr extract obtained by it
Element, rutin, myricetin, chlorogenic acid, and this total amount accounts for the 40~70% of the extract gross weight.
Method according to a first aspect of the present invention, apiolin, quercitrin are included in the Gynura procumbens (Lour.) Merr extract obtained by it
Element, rutin, myricetin, chlorogenic acid, and this total amount accounts for the 50~60% of the extract gross weight.
Further, second aspect of the present invention provides a kind of Gynura procumbens (Lour.) Merr extract, wherein including apiolin, quercitrin
Element, rutin, myricetin, chlorogenic acid, and this total amount accounts for the 40~70% of the extract gross weight.
It is Gynura procumbens (Lour.) Merr extract according to a second aspect of the present invention, wherein apiolin, Quercetin, rutin, myricetin, green
Ortho acid total amount accounts for the 50~60% of the extract gross weight.
Gynura procumbens (Lour.) Merr extract according to a second aspect of the present invention, it is prepared by the method included the following steps
's：
(1) provide through the dry Gynura procumbens (Lour.) Merr to moisture less than 10%, be ground into most coarse powder, obtain Gynura procumbens (Lour.) Merr
Dry powder；
(2) make Gynura procumbens (Lour.) Merr dry powder add 5~8 times amount water immersion 1~3 it is small when, then decoct 30~120 minutes, filter
Take filtrate；Filter residue adds the water of 3~6 times of amounts to decoct again 30~120 minutes, leaching filtrate；Merge filtrate twice；
(3) white bole and propane diols are added into filtrate, is stirred evenly, when standing 2~4 is small, is depressurized at 50~70 DEG C
It is concentrated into 1/5 to 1/3 volume；Ethanol is added to stir evenly to alcohol content up to 50~60%, when standing 2~4 is small, filter to remove
Solid content；
(4) be concentrated under reduced pressure into no alcohol taste at 50~70 DEG C, then it is spray-dried be less than 5% to moisture, put down
Sleeping chrysanthemum Notogineng Extract.
In Gynura procumbens (Lour.) Merr extract according to a second aspect of the present invention, wherein step (1), the drying is selected from as follows
Drying mode：It is dried up or heat drying.
In Gynura procumbens (Lour.) Merr extract according to a second aspect of the present invention, wherein step (1), the drying is dry to water
Content is divided to be less than 8%.
In Gynura procumbens (Lour.) Merr extract according to a second aspect of the present invention, wherein step (2), for the first time plus 5~6 times amount
Water soaks.
In Gynura procumbens (Lour.) Merr extract according to a second aspect of the present invention, wherein step (2), for the first time plus 5~6 times amount
When water immersion 1~2 is small.
In Gynura procumbens (Lour.) Merr extract according to a second aspect of the present invention, wherein step (2), for the first time plus 5~6 times amount
When water immersion 1~2 is small, then decoct 30~60 minutes.
In Gynura procumbens (Lour.) Merr extract according to a second aspect of the present invention, wherein step (2), filter residue adds the water of 3~4 times of amounts
Decoct again.
In Gynura procumbens (Lour.) Merr extract according to a second aspect of the present invention, wherein step (2), filter residue adds the water of 3~4 times of amounts
Decoct again 30~60 minutes.
In Gynura procumbens (Lour.) Merr extract according to a second aspect of the present invention, wherein step (3), the amount of white bole is amount of filtrate
0.5~1% (g/100ml).
In Gynura procumbens (Lour.) Merr extract according to a second aspect of the present invention, wherein step (3), the amount of white bole is amount of filtrate
0.5~0.75% (g/100ml).
In Gynura procumbens (Lour.) Merr extract according to a second aspect of the present invention, wherein step (3), propane diols is 1,2- the third two
Alcohol.
In Gynura procumbens (Lour.) Merr extract according to a second aspect of the present invention, wherein step (3), the amount of propane diols is amount of filtrate
0.2~0.6% (g/100ml).
In Gynura procumbens (Lour.) Merr extract according to a second aspect of the present invention, wherein step (3), the amount of propane diols is amount of filtrate
0.2~0.4% (g/100ml).
In Gynura procumbens (Lour.) Merr extract according to a second aspect of the present invention, wherein step (3), in filtrate add white bole and
Propane diols, stirs evenly, when standing 2~3 is small.
In Gynura procumbens (Lour.) Merr extract according to a second aspect of the present invention, wherein step (3), in filtrate add white bole and
Propane diols, stirs evenly, and when standing 2~3 is small, is concentrated under reduced pressure at 50~60 DEG C.
In Gynura procumbens (Lour.) Merr extract according to a second aspect of the present invention, wherein step (3), in filtrate add white bole and
Propane diols, stirs evenly, and when standing 2~3 is small, 1/5 to 1/4 volume is concentrated under reduced pressure at 50~60 DEG C.
In Gynura procumbens (Lour.) Merr extract according to a second aspect of the present invention, wherein step (3), add ethanol to alcohol content up to 50
~55%.
In Gynura procumbens (Lour.) Merr extract according to a second aspect of the present invention, wherein step (3), add ethanol to alcohol content up to 50
~55%, stir evenly, when standing 2~3 is small, filter to remove solid content.
In Gynura procumbens (Lour.) Merr extract according to a second aspect of the present invention, wherein step (4), depressurized at 55~65 DEG C dense
It is reduced to no alcohol taste.
In Gynura procumbens (Lour.) Merr extract according to a second aspect of the present invention, wherein step (4), depressurized at 55~65 DEG C dense
Be reduced to no alcohol taste, then it is spray-dried be less than 4% to moisture, obtain Gynura procumbens (Lour.) Merr extract.
Gynura procumbens (Lour.) Merr extract according to a second aspect of the present invention, includes celery in the Gynura procumbens (Lour.) Merr extract
Element, Quercetin, rutin, myricetin, chlorogenic acid.
Gynura procumbens (Lour.) Merr extract according to a second aspect of the present invention, apiolin, Mongolian oak in the Gynura procumbens (Lour.) Merr extract
Pi Su, rutin, myricetin, chlorogenic acid overall recovery are more than 50%.Term " rate of recovery " refers to obtained by the method for the present invention
Gynura procumbens (Lour.) Merr extract in five kinds of chemical substance total amounts account for and prepare in Gynura procumbens (Lour.) Merr dry powder used in the extract five kinds of changes
The percentage of material total amount is learned, it reflects that five kinds of chemical substances reach the whole mistake of extract of the present invention from dry powder through the method for the present invention
The rate of recovery of journey, this rate of recovery is higher to show that the method for the present invention is higher for the efficiency for putting forward this five kinds of chemical substances.
Gynura procumbens (Lour.) Merr extract according to a second aspect of the present invention, apiolin, Mongolian oak in the Gynura procumbens (Lour.) Merr extract
Pi Su, rutin, myricetin, chlorogenic acid overall recovery are more than 60%.
Gynura procumbens (Lour.) Merr extract according to a second aspect of the present invention, apiolin, Mongolian oak in the Gynura procumbens (Lour.) Merr extract
Pi Su, rutin, myricetin, chlorogenic acid overall recovery are more than 70%.
Gynura procumbens (Lour.) Merr extract according to a second aspect of the present invention, apiolin, Mongolian oak in the Gynura procumbens (Lour.) Merr extract
Pi Su, rutin, myricetin, chlorogenic acid overall recovery are more than 75%.
Gynura procumbens (Lour.) Merr extract according to a second aspect of the present invention, apiolin, Mongolian oak in the Gynura procumbens (Lour.) Merr extract
Pi Su, rutin, myricetin, chlorogenic acid total amount account for the 40~70% of the extract gross weight.This hair of this accounting as shown by data
The content of the bright extract obtained middle target chemical substances of method is high.
Gynura procumbens (Lour.) Merr extract according to a second aspect of the present invention, apiolin, Mongolian oak in the Gynura procumbens (Lour.) Merr extract
Pi Su, rutin, myricetin, chlorogenic acid total amount account for the 50~60% of the extract gross weight.
Further, third aspect present invention provides a kind of composition, wherein comprising Gynura procumbens (Lour.) Merr extract, and
The acceptable auxiliary material of physiology.
Composition according to a third aspect of the present invention, wherein the auxiliary material is selected from：Diluent such as water, sucrose, starch, paste
Essence, lactose etc., Flavouring agents such as aspartame, saccharin sodium, acesulfame potassium.
Composition according to a third aspect of the present invention, it is in tablet, granule, the dosage form of oral solution.This
A little dosage forms can be by the way that well known to a person skilled in the art method, the Gynura procumbens (Lour.) Merr extract obtained by the present invention be prepared into
Arrive.When preparing these dosage forms, it is acceptable the physiology can be added thereto according to method well known in the art
Auxiliary material.
Composition according to a third aspect of the present invention, wherein the Gynura procumbens (Lour.) Merr extract is by including the following steps
What method was prepared：
(1) provide through the dry Gynura procumbens (Lour.) Merr to moisture less than 10%, be ground into most coarse powder, obtain Gynura procumbens (Lour.) Merr
Dry powder；
(2) make Gynura procumbens (Lour.) Merr dry powder add 5~8 times amount water immersion 1~3 it is small when, then decoct 30~120 minutes, filter
Take filtrate；Filter residue adds the water of 3~6 times of amounts to decoct again 30~120 minutes, leaching filtrate；Merge filtrate twice；
(3) white bole and propane diols are added into filtrate, is stirred evenly, when standing 2~4 is small, is depressurized at 50~70 DEG C
It is concentrated into 1/5 to 1/3 volume；Ethanol is added to stir evenly to alcohol content up to 50~60%, when standing 2~4 is small, filter to remove
Solid content；
(4) be concentrated under reduced pressure into no alcohol taste at 50~70 DEG C, then it is spray-dried be less than 5% to moisture, put down
Sleeping chrysanthemum Notogineng Extract.
In composition according to a third aspect of the present invention, wherein step (1), the drying is drying side chosen from the followings
Formula：It is dried up or heat drying.
In composition according to a third aspect of the present invention, wherein step (1), the drying is that drying to moisture is less than
8%.
In composition according to a third aspect of the present invention, wherein step (2), for the first time plus 5~6 times of water measured soak.
In composition according to a third aspect of the present invention, wherein step (2), for the first time plus 5~6 times of water measured soak 1~2
Hour.
In composition according to a third aspect of the present invention, wherein step (2), for the first time plus 5~6 times of water measured soak 1~2
Hour, then decoct 30~60 minutes.
In composition according to a third aspect of the present invention, wherein step (2), filter residue adds the water of 3~4 times of amounts to decoct again.
In composition according to a third aspect of the present invention, wherein step (2), filter residue add the water of 3~4 times of amounts decoct 30 again~
60 minutes.
In composition according to a third aspect of the present invention, wherein step (3), the amount of white bole is the 0.5~1% of amount of filtrate
(g/100ml)。
In composition according to a third aspect of the present invention, wherein step (3), the amount of white bole for amount of filtrate 0.5~
0.75% (g/100ml).
In composition according to a third aspect of the present invention, wherein step (3), propane diols is 1,2-PD.
In composition according to a third aspect of the present invention, wherein step (3), the amount of propane diols for amount of filtrate 0.2~
0.6% (g/100ml).
In composition according to a third aspect of the present invention, wherein step (3), the amount of propane diols for amount of filtrate 0.2~
0.4% (g/100ml).
In composition according to a third aspect of the present invention, wherein step (3), white bole and propane diols are added in filtrate, is stirred
Mix uniformly, when standing 2~3 is small.
In composition according to a third aspect of the present invention, wherein step (3), white bole and propane diols are added in filtrate, is stirred
Mix uniformly, when standing 2~3 is small, be concentrated under reduced pressure at 50~60 DEG C.
In composition according to a third aspect of the present invention, wherein step (3), white bole and propane diols are added in filtrate, is stirred
Mix uniformly, when standing 2~3 is small, 1/5 to 1/4 volume is concentrated under reduced pressure at 50~60 DEG C.
In composition according to a third aspect of the present invention, wherein step (3), add ethanol to alcohol content up to 50~55%.
In composition according to a third aspect of the present invention, wherein step (3), ethanol is added to be stirred to alcohol content up to 50~55%
Mix uniformly, when standing 2~3 is small, filter to remove solid content.
In composition according to a third aspect of the present invention, wherein step (4), no alcohol is concentrated under reduced pressure at 55~65 DEG C
Taste.
In composition according to a third aspect of the present invention, wherein step (4), no alcohol is concentrated under reduced pressure at 55~65 DEG C
Taste, then it is spray-dried to moisture be less than 4%, obtain Gynura procumbens (Lour.) Merr extract.
Composition according to a third aspect of the present invention, apiolin, Quercetin, reed are included in the Gynura procumbens (Lour.) Merr extract
Fourth, myricetin, chlorogenic acid.
Composition according to a third aspect of the present invention, apiolin in the Gynura procumbens (Lour.) Merr extract, Quercetin, rutin,
Myricetin, chlorogenic acid overall recovery are more than 50%.Term " rate of recovery " refers to the prostrate chrysanthemum three obtained by the method for the present invention
Five kinds of chemical substance total amounts, which account for, in seven extracts prepares five kinds of chemical substance total amounts in Gynura procumbens (Lour.) Merr dry powder used in the extract
Percentage, it reflects that five kinds of chemical substances reach the recycling of extract whole process of the present invention from dry powder through the method for the present invention
Rate, this rate of recovery is higher to show that the method for the present invention is higher for the efficiency for putting forward this five kinds of chemical substances.
Composition according to a third aspect of the present invention, apiolin in the Gynura procumbens (Lour.) Merr extract, Quercetin, rutin,
Myricetin, chlorogenic acid overall recovery are more than 60%.
Composition according to a third aspect of the present invention, apiolin in the Gynura procumbens (Lour.) Merr extract, Quercetin, rutin,
Myricetin, chlorogenic acid overall recovery are more than 70%.
Composition according to a third aspect of the present invention, apiolin in the Gynura procumbens (Lour.) Merr extract, Quercetin, rutin,
Myricetin, chlorogenic acid overall recovery are more than 75%.
Composition according to a third aspect of the present invention, apiolin in the Gynura procumbens (Lour.) Merr extract, Quercetin, rutin,
Myricetin, chlorogenic acid total amount account for the 40~70% of the extract gross weight.Obtained by this accounting as shown by data the method for the present invention
The content of target chemical substances is high in extract.
Composition according to a third aspect of the present invention, apiolin in the Gynura procumbens (Lour.) Merr extract, Quercetin, rutin,
Myricetin, chlorogenic acid total amount account for the 50~60% of the extract gross weight.
Further, fourth aspect present invention provides the prostrate chrysanthemum obtained by first aspect present invention any embodiment
Gynura procumbens (Lour.) Merr extract described in Notogineng Extract or second aspect of the present invention any embodiment is being prepared for improving the heart
Vascular function, improve the immunity of the human body and/or antitumor product in purposes.
In the step of above-mentioned preparation method of the invention, although the specific steps of its description are in some details or language
The step of described in preparation example in description with following detailed description part, is otherwise varied, however, people in the art
The detailed disclosure of member's full text according to the present invention can summarize approach described above step completely.
Any embodiment of the either side of the present invention, can be combined with other embodiments, as long as they are not
Contradiction occurs.In addition, in any embodiment of either side of the present invention, any technical characteristic can be adapted for other realities
The technical characteristic in scheme is applied, as long as they are not in contradiction.The invention will be further described below.
All documents recited in the present invention, their full content are incorporated herein by reference, and if these are literary
When offering expressed implication and the inconsistent present invention, the statement of the present invention is subject to.In addition, the various terms that use of the present invention and
Phrase has well known to a person skilled in the art general sense, nonetheless, the present invention remain desirable at this to these terms and
Phrase is described in more detail and explains, the term and phrase referred to is if any inconsistent with common art-recognized meanings, with institute's table of the present invention
Subject to the implication stated.
It has been found that excellent biological activity is presented in extract of the present invention, these biological activities include but not limited to：
Improve cardiovascular and cerebrovascular symptoms, blood pressure lowering, hypoglycemic, reducing blood lipid；Toxin expelling, beauty, beauty treatment, anti-aging；Improvement function of immune system,
Improve immunity；Suppress growth of tumour cell.In addition, the present invention, by specific preparation process, obtained Gynura procumbens (Lour.) Merr carries
Take thing that excellent Chemical Characteristics are presented, particularly some typical activity chemical material components are in extract of the present invention or group
There is high content in compound.Particularly have now surprisingly been found that, in the concentration process for preparing extract of the present invention, addition
A small amount of white bole is combined with both propane diols, above-mentioned typical activity chemical substance is combined in the present invention
Be enriched with thing, due to white bole be in itself a kind of conventional pharmaceutic adjuvant and its it is not soluble in water be also insoluble in ethanol,
Can be cleared by its by simple filters in the method for the present invention；And propane diols is since its additive amount is small, and it is a kind of
Extremely safe and common medicinal fear material, even if it has remnants, this small amount of remnants completely will not in extract of the present invention
Any detrimental effect is caused to body.For example, in first complementary testing, the method that respectively refers to embodiment 1-5 is different
Be only that white bole is not added in step (3), apiolin, Quercetin, rutin, myricetin, chlorogenic acid in five batches of extracts of gained
Five overall recoverys 33~41%, five total amounts account for the 23~27% of extract gross weight (such as thus with reference to the gained of embodiment 1
Extract (it can be labeled as B11 in the present invention) two percentage is respectively 36.3% and 24.7%).In second supplement
In experiment, the method that respectively refers to embodiment 1-5, different is only that propane diols is not added in step (3), five batches of extracts of gained
Middle apiolin, Quercetin, rutin, myricetin, chlorogenic acid overall recovery 36~43%, five total amounts account for extract gross weight
21~25% (such as thus with reference to extract obtained (it can be labeled as B21 in the present invention) two percentage of embodiment 1
Respectively 39.5% and 22.4%).In the 3rd complementary testing, the method that respectively refers to embodiment 1-5, different is only step
Suddenly white bole is not added in (3), apiolin, Quercetin, rutin, myricetin, chlorogenic acid always return in five batches of extracts of gained
Yield 31~37%, five total amounts account for extract gross weight 19~26% (such as thus with reference to embodiment 1 it is extract obtained
(it can be labeled as B31 in the present invention) two percentage is respectively 34.6% and 21.3%).
The present invention extracts Gynura procumbens (Lour.) Merr through the method for the present invention to obtain Gynura procumbens (Lour.) Merr extract, optionally further by it
Composition is made for example in the composition of dosage form, either excellent chemical property or life is presented in composition to these extracts
Thing property.
Embodiment
The present invention can be further described by the following examples, however, the scope of the present invention and unlimited
In following embodiments.One of skill in the art, can be with it is understood that on the premise of without departing substantially from the spirit and scope of the present invention
Various change and modification are carried out to the present invention.The present invention carries out the material and test method that are arrived used in experiment general
And/or specific description.Although for realize many materials used in the object of the invention and operating method be it is known in the art that
But the present invention is still described in detail as far as possible herein.
Test example 1：Apiolin, Quercetin, rutin, myricetin, chlorogenic acid in Gynura procumbens (Lour.) Merr dry powder or its extract
Assay method
With reference to literature methods such as You Jiaojiao, (especially tender etc., HPLC-MS/MS methods measure 9 in Gynura procumbens (Lour.) Merr water extract at the same time
Kind principle active component, Chinese herbal medicine, 02 phase in 2017), measure Gynura procumbens (Lour.) Merr dry powder and apiolin, quercitrin in its extract
Element, rutin, myricetin, chlorogenic acid, protocatechuic acid, p-Coumaric Acid, caffeic acid, the content of nine kinds of materials of Kaempferol, using celery
Element, Quercetin, rutin, myricetin, the amount of five kinds of Exemplary chemical materials of chlorogenic acid carry out judging extract of the present invention or prostrate
Chrysanthemum pseudo-ginseng dry powder.
Test example 2：Influence of the Gynura procumbens (Lour.) Merr extract to mouse blood sugar and blood fat
With reference to literature method (Zheng Guodong etc., influence of the Gynura procumbens (Lour.) Merr to mouse blood sugar and blood fat, the modern times of Zheng Guodong etc.
Food science and technology, 12 phases in 2013), for six extracts of Ex1, Ex2, Ex3, B11, B21, B31, them are measured to mouse blood sugar
With the influence of blood fat, six extract doses are 20g/kg weight in terms of Gynura procumbens (Lour.) Merr dry powder, once a day, are continuously given
12 weeks, while control group is set, every group of 10 animals.The results show：Mouse weight is increased by six extracts and organ weights do not have
There is significant change；Ex1, Ex2, Ex3 notable (p compared with control group<0.01) triglycerides (TG), the total courage reduced in blood is consolidated
The concentration of TG in alcohol, the cholesterol of low-density lipoprotein and blood glucose and liver, the TG significantly (p in excrement<0.01) increase, notable (p
<0.01) vigor and mRNA and protein expression level of hydroxymethyl glutaryl CoA reductases (HMGCR), the mRNA of Glut4 are suppressed
Substantially increase with protein expression；B11, B21, B31 are compared with control group, notable (p<0.05) triglycerides in blood is reduced
(TG), in T-CHOL, the cholesterol of low-density lipoprotein and blood glucose and liver TG concentration, the TG in excrement has increased slightly but nothing
Significant difference, notable (p<0.05) vigor and mRNA and protein expression of hydroxymethyl glutaryl CoA reductases (HMGCR) are suppressed
Level, the mRNA and protein expression no significant difference of Glut4.Result above shows that Gynura procumbens (Lour.) Merr extract can pass through adjusting
The mRNA and protein expression of HMGCR and Glut4, and suppress enteron aisle and TG is absorbed, reach hypoglycemic lipid-lowering effect.
In addition, also in the experiment for suppressing solid tumor, it was confirmed that tri- extracts of Ex1, Ex2, Ex3 have obvious tumor suppression
Effect, and the tumor-inhibiting action of tri- kinds of extracts of B11, B21, B31 of about 3.7~4.3 times of amounts and Ex1, Ex2, Ex3 are equivalent, table
The tumor-inhibiting action of bright Ex1, Ex2, Ex3 are significantly stronger than B11, B21, B31 three.
Test example 3：Gynura procumbens (Lour.) Merr extract Study on Its Antioxidant Activity in Vitro
With reference to Meng Xing etc. literature method (Meng Xing etc., Gynura procumbens (Lour.) Merr extract Study on Its Antioxidant Activity in Vitro, food with
Machinery, 10 phases in 2016) investigate Gynura procumbens (Lour.) Merr extract Ex1, Ex2, Ex3 antioxidation activity in vitro effect.As a result show
Show that three kinds of extracts are respectively provided with significant antioxidation activity in vitro, particularly their antioxidation activity than it in B11, B21,
Tri- kinds of extracts of B31 are clearly more powerful, e.g., from about 3.3~5.1 times amount tri- kinds of extracts of B11, B21, B31 antioxidation activity with
Ex1, Ex2, Ex3 are equivalent.
Embodiment 1：The preparation of Gynura procumbens (Lour.) Merr extract
(1) Gynura procumbens (Lour.) Merr through drying (drying) to moisture 6% is provided, most coarse powder is ground into, chrysanthemum three must be lieed down
Seven dry powder；
(2) make Gynura procumbens (Lour.) Merr dry powder add 6 times amount water immersion 2 it is small when, then decoct 45 minutes, leaching filtrate；Filter residue
The water of 4 times of amounts is added to decoct again 45 minutes, leaching filtrate；Merge filtrate twice；
(3) white bole (in an amount of from the 0.6% of amount of filtrate) and propane diols are added into filtrate (in an amount of from amount of filtrate
0.3%), stir evenly, when standing 3 is small, 1/4 volume is concentrated under reduced pressure at 55 DEG C；Ethanol is added to be stirred to alcohol content up to 55%
Uniformly, when standing 3 is small, filter to remove solid content；
(4) be concentrated under reduced pressure into no alcohol taste at 60 DEG C, then it is spray-dried be less than 4% to moisture, obtain lying down chrysanthemum
Notogineng Extract.It can be labeled as Ex1 in the present invention.
Apiolin, Quercetin, rutin, myricetin, chlorogenic acid overall recovery 81% in extract.
Apiolin, Quercetin, rutin, myricetin, chlorogenic acid total amount account for the 56% of extract gross weight in extract.
In addition, the present inventor prepares Gynura procumbens (Lour.) Merr extract with reference to the method for the prior art retrieved, tie
Fruit shows the extract obtained middle apiolin of these methods, Quercetin, rutin, myricetin, chlorogenic acid overall recovery 30
In the range of~40%, apiolin, Quercetin, rutin, myricetin, chlorogenic acid total amount account for extract gross weight in extract
24~37%.Such as according to the side in CN106924317A (Chinese Patent Application No. 201511023838X) specification its [0055]
Method prepares extract, and five kinds of chemical composition overall recoverys are 33.2, and five kinds of chemical composition total amounts account for extract gross weight
29.2%.In another example according to the side in CN106924315A (Chinese Patent Application No. 2015110215485) specification its [0050]
Method prepares extract, and five kinds of chemical composition overall recoverys are 36.4, and five kinds of chemical composition total amounts account for extract gross weight
33.1%.
Embodiment 2：The preparation of Gynura procumbens (Lour.) Merr extract
(1) Gynura procumbens (Lour.) Merr through drying (heat drying) to moisture 8% is provided, most coarse powder is ground into, must lie down
Chrysanthemum pseudo-ginseng dry powder；
(2) make Gynura procumbens (Lour.) Merr dry powder add 5 times amount water immersion 2.5 it is small when, then decoct 30 minutes, leaching filtrate；Filter
Slag adds the water of 3.5 times of amounts to decoct again 45 minutes, leaching filtrate；Merge filtrate twice；
(3) white bole (in an amount of from the 0.5% of amount of filtrate) and propane diols are added into filtrate (in an amount of from amount of filtrate
0.3%), stir evenly, when standing 2 is small, 1/3 volume is concentrated under reduced pressure at 60 DEG C；Ethanol is added to be stirred to alcohol content up to 53%
Uniformly, when standing 2 is small, filter to remove solid content；
(4) be concentrated under reduced pressure into no alcohol taste at 65 DEG C, then it is spray-dried be less than 4% to moisture, obtain lying down chrysanthemum
Notogineng Extract.It can be labeled as Ex2 in the present invention.
Apiolin, Quercetin, rutin, myricetin, chlorogenic acid overall recovery 77% in extract.
Apiolin, Quercetin, rutin, myricetin, chlorogenic acid total amount account for the 59% of extract gross weight in extract.
Embodiment 3：The preparation of Gynura procumbens (Lour.) Merr extract
(1) Gynura procumbens (Lour.) Merr through drying (air-dried) to moisture 7% is provided, most coarse powder is ground into, chrysanthemum three must be lieed down
Seven dry powder；
(2) make Gynura procumbens (Lour.) Merr dry powder add 7 times amount water immersion 3 it is small when, then decoct 60 minutes, leaching filtrate；Filter residue
The water of 5 times of amounts is added to decoct again 30 minutes, leaching filtrate；Merge filtrate twice；
(3) white bole (in an amount of from the 0.75% of amount of filtrate) and propane diols are added into filtrate (in an amount of from amount of filtrate
0.4%), stir evenly, when standing 4 is small, 1/5 volume is concentrated under reduced pressure at 50 DEG C；Ethanol is added to be stirred to alcohol content up to 55%
Uniformly, when standing 4 is small, filter to remove solid content；
(4) be concentrated under reduced pressure into no alcohol taste at 55 DEG C, then it is spray-dried be less than 4% to moisture, obtain lying down chrysanthemum
Notogineng Extract.It can be labeled as Ex3 in the present invention.
Apiolin, Quercetin, rutin, myricetin, chlorogenic acid overall recovery 71% in extract.
Apiolin, Quercetin, rutin, myricetin, chlorogenic acid total amount account for the 58% of extract gross weight in extract.
Embodiment 4：The preparation of Gynura procumbens (Lour.) Merr extract
(1) Gynura procumbens (Lour.) Merr through drying (drying) to moisture 5% is provided, most coarse powder is ground into, chrysanthemum three must be lieed down
Seven dry powder；
(2) make Gynura procumbens (Lour.) Merr dry powder add 8 times amount water immersion 2 it is small when, then decoct 120 minutes, leaching filtrate；Filter residue
The water of 3 times of amounts is added to decoct again 30 minutes, leaching filtrate；Merge filtrate twice；
(3) white bole (in an amount of from the 1% of amount of filtrate) and propane diols are added into filtrate (in an amount of from amount of filtrate
0.2%), stir evenly, when standing 3.5 is small, 1/3 volume is concentrated under reduced pressure at 70 DEG C；Ethanol is added to be stirred to alcohol content up to 50%
Mix uniformly, when standing 4 is small, filter to remove solid content；
(4) be concentrated under reduced pressure into no alcohol taste at 50 DEG C, then it is spray-dried be less than 5% to moisture, obtain lying down chrysanthemum
Notogineng Extract.It can be labeled as Ex4 in the present invention.
Apiolin, Quercetin, rutin, myricetin, chlorogenic acid overall recovery 83% in extract.
Apiolin, Quercetin, rutin, myricetin, chlorogenic acid total amount account for the 51% of extract gross weight in extract.
Embodiment 5：The preparation of Gynura procumbens (Lour.) Merr extract
(1) Gynura procumbens (Lour.) Merr through drying (drying) to moisture 6% is provided, most coarse powder is ground into, chrysanthemum three must be lieed down
Seven dry powder；
(2) make Gynura procumbens (Lour.) Merr dry powder add 5 times amount water immersion 1 it is small when, then decoct 90 minutes, leaching filtrate；Filter residue
The water of 4 times of amounts is added to decoct again 60 minutes, leaching filtrate；Merge filtrate twice；
(3) white bole (in an amount of from the 0.8% of amount of filtrate) and propane diols are added into filtrate (in an amount of from amount of filtrate
0.3%), stir evenly, when standing 2.5 is small, 1/4 volume is concentrated under reduced pressure at 55 DEG C；Ethanol is added to be stirred to alcohol content up to 60%
Mix uniformly, when standing 2 is small, filter to remove solid content；
(4) be concentrated under reduced pressure into no alcohol taste at 70 DEG C, then it is spray-dried be less than 4% to moisture, obtain lying down chrysanthemum
Notogineng Extract.It can be labeled as Ex5 in the present invention.
Apiolin, Quercetin, rutin, myricetin, chlorogenic acid overall recovery 79% in extract.
Apiolin, Quercetin, rutin, myricetin, chlorogenic acid total amount account for the 54% of extract gross weight in extract.
Composition example 1：The preparation of Gynura procumbens (Lour.) Merr composition
Example 1 is extract obtained to embodiment 5 respectively, and oral administration solution is configured to water, extract wherein dense
Spend for 10%, add 0.2% aspartame as sweetener, obtain the composition of five kinds of drink forms.
Composition example 2：The preparation of Gynura procumbens (Lour.) Merr composition
Example 1 is extract obtained to embodiment 5 respectively, adds the sucrose and 2 times of dextrin and 0.05% measured of 3 times of amounts
Aspartame is uniformly mixed as sweetener, and particle is made, and obtains the composition of five kinds of granular forms.
Composition example 3：The preparation of Gynura procumbens (Lour.) Merr composition
Example 1 is extract obtained to embodiment 5 respectively, adds the dextrin of 3 times of sucrose measured and 2 times of amounts to be uniformly mixed,
It is wetting agent wet granular with water, it is dry, 0.2% magnesium stearate is added in dry particl, is uniformly mixed, tabletting, every weight 0.3~
0.5g, obtains the composition of five kinds of tablet forms.
Claims (10)
1. preparing the method for Gynura procumbens (Lour.) Merr extract, it includes the following steps：
(1) provide through the dry Gynura procumbens (Lour.) Merr to moisture less than 10%, be ground into most coarse powder, obtain Gynura procumbens (Lour.) Merr and do
Powder；
(2) make Gynura procumbens (Lour.) Merr dry powder add 5~8 times amount water immersion 1~3 it is small when, then decoct 30~120 minutes, leaching filter
Liquid；Filter residue adds the water of 3~6 times of amounts to decoct again 30~120 minutes, leaching filtrate；Merge filtrate twice；
(3) white bole and propane diols are added into filtrate, is stirred evenly, when standing 2~4 is small, is concentrated under reduced pressure at 50~70 DEG C
To 1/5 to 1/3 volume；Ethanol is added to stir evenly to alcohol content up to 50~60%, when standing 2~4 is small, filter to remove solid
Thing；
(4) be concentrated under reduced pressure into no alcohol taste at 50~70 DEG C, then it is spray-dried be less than 5% to moisture, obtain lying down chrysanthemum
Notogineng Extract.
2. the method according to claim 1, wherein：
In step (1), the drying is drying mode chosen from the followings：It is dried up or heat drying；
In step (1), the drying is that drying to moisture is less than 8%；
In step (2), for the first time plus 5~6 times of water measured soak；
In step (2), when for the first time plus 5~6 times of water immersions 1~2 measured are small；
In step (2), when for the first time plus 5~6 times of water immersions 1~2 measured are small, then decoct 30~60 minutes；
In step (2), filter residue adds the water of 3~4 times of amounts to decoct again；
In step (2), filter residue adds the water of 3~4 times of amounts to decoct again 30~60 minutes.
3. the method according to claim 1, wherein：
In step (3), the amount of white bole is 0.5~1% (g/100ml) of amount of filtrate；
In step (3), the amount of white bole is 0.5~0.75% (g/100ml) of amount of filtrate；
In step (3), propane diols is 1,2-PD；
In step (3), the amount of propane diols is 0.2~0.6% (g/100ml) of amount of filtrate；
In step (3), the amount of propane diols is 0.2~0.4% (g/100ml) of amount of filtrate；
In step (3), white bole and propane diols are added in filtrate, is stirred evenly, when standing 2~3 is small；
In step (3), white bole and propane diols are added in filtrate, is stirred evenly, when standing 2~3 is small, depressurized at 50~60 DEG C
Concentration；
In step (3), white bole and propane diols are added in filtrate, is stirred evenly, when standing 2~3 is small, depressurized at 50~60 DEG C
It is concentrated into 1/5 to 1/4 volume；
In step (3), add ethanol to alcohol content up to 50~55%；
In step (3), ethanol is added to stir evenly to alcohol content up to 50~55%, when standing 2~3 is small, filter to remove solid
Thing；
In step (4), no alcohol taste is concentrated under reduced pressure at 55~65 DEG C；
In step (4), be concentrated under reduced pressure into no alcohol taste at 55~65 DEG C, then it is spray-dried be less than 4% to moisture, obtain
Gynura procumbens (Lour.) Merr extract.
4. the method according to claim 1, wherein：
Apiolin, Quercetin, rutin, myricetin, chlorogenic acid are included in Gynura procumbens (Lour.) Merr extract obtained by it；
Apiolin, Quercetin, rutin, myricetin, chlorogenic acid overall recovery in Gynura procumbens (Lour.) Merr extract obtained by it
More than 50%；
Apiolin, Quercetin, rutin, myricetin, chlorogenic acid overall recovery in Gynura procumbens (Lour.) Merr extract obtained by it
More than 60%；
Apiolin, Quercetin, rutin, myricetin, chlorogenic acid overall recovery in Gynura procumbens (Lour.) Merr extract obtained by it
More than 70%；Such as overall recovery can reach 70~85% scope be particularly can reach 75~85% scope；
Apiolin, Quercetin, rutin, myricetin, chlorogenic acid overall recovery in Gynura procumbens (Lour.) Merr extract obtained by it
More than 75%.
5. the method according to claim 1, wherein：
Comprising apiolin, Quercetin, rutin, myricetin, chlorogenic acid in Gynura procumbens (Lour.) Merr extract obtained by it, and this
Total amount accounts for the 40~70% of the extract gross weight；
Comprising apiolin, Quercetin, rutin, myricetin, chlorogenic acid in Gynura procumbens (Lour.) Merr extract obtained by it, and this
Total amount accounts for the 50~60% of the extract gross weight.
6. a kind of Gynura procumbens (Lour.) Merr extract, wherein comprising apiolin, Quercetin, rutin, myricetin, chlorogenic acid, and this
Total amount accounts for the 40~70% of the extract gross weight；Wherein apiolin, Quercetin, rutin, myricetin, chlorogenic acid total amount account for
The 50~60% of the extract gross weight；The Gynura procumbens (Lour.) Merr extract is prepared by the method included the following steps：
(1) provide through the dry Gynura procumbens (Lour.) Merr to moisture less than 10%, be ground into most coarse powder, obtain Gynura procumbens (Lour.) Merr and do
Powder；
(2) make Gynura procumbens (Lour.) Merr dry powder add 5~8 times amount water immersion 1~3 it is small when, then decoct 30~120 minutes, leaching filter
Liquid；Filter residue adds the water of 3~6 times of amounts to decoct again 30~120 minutes, leaching filtrate；Merge filtrate twice；
(3) white bole and propane diols are added into filtrate, is stirred evenly, when standing 2~4 is small, is concentrated under reduced pressure at 50~70 DEG C
To 1/5 to 1/3 volume；Ethanol is added to stir evenly to alcohol content up to 50~60%, when standing 2~4 is small, filter to remove solid
Thing；
(4) be concentrated under reduced pressure into no alcohol taste at 50~70 DEG C, then it is spray-dried be less than 5% to moisture, obtain lying down chrysanthemum
Notogineng Extract.
A kind of 7. composition, wherein including Gynura procumbens (Lour.) Merr extract, and the acceptable auxiliary material of physiology.
8. composition according to claim 7, wherein the auxiliary material is selected from：Diluent such as water, sucrose, starch, dextrin, lactose
Deng Flavouring agents such as aspartame, saccharin sodium, acesulfame potassium.
9. composition according to claim 7, it is in tablet, granule, the dosage form of oral solution.
10. lie down chrysanthemum described in Gynura procumbens (Lour.) Merr extract or claim made from any one of claim 1-5 the method
Notogineng Extract prepare be used for improve cardiovascular function, improve the immunity of the human body and/or antitumor product in purposes.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201711323059.4A CN107951915A (en) | 2017-12-12 | 2017-12-12 | For improving cardiovascular raising immunity and antitumor Gynura procumbens (Lour.) Merr extract |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201711323059.4A CN107951915A (en) | 2017-12-12 | 2017-12-12 | For improving cardiovascular raising immunity and antitumor Gynura procumbens (Lour.) Merr extract |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112057486A (en) * | 2019-06-11 | 2020-12-11 | 百岳特生物技术（上海）有限公司 | Application of gynura procumbens extract in preparing composition for improving sleep quality and preparing composition for protecting health and promoting blood circulation |
| CN113201081A (en) * | 2021-06-11 | 2021-08-03 | 珠海俊一健生物医药科技有限公司 | Gynura procumbens polysaccharide with immunological activity and preparation method and application thereof |
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| CN101032538A (en) * | 2007-04-08 | 2007-09-12 | 谢平 | Method of preparing substances extracted from Gynura procumbens (Lour.) Merr. and the application |
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| CN101032538A (en) * | 2007-04-08 | 2007-09-12 | 谢平 | Method of preparing substances extracted from Gynura procumbens (Lour.) Merr. and the application |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112057486A (en) * | 2019-06-11 | 2020-12-11 | 百岳特生物技术（上海）有限公司 | Application of gynura procumbens extract in preparing composition for improving sleep quality and preparing composition for protecting health and promoting blood circulation |
| CN113201081A (en) * | 2021-06-11 | 2021-08-03 | 珠海俊一健生物医药科技有限公司 | Gynura procumbens polysaccharide with immunological activity and preparation method and application thereof |
| CN113201081B (en) * | 2021-06-11 | 2023-01-06 | 珠海俊一健生物医药科技有限公司 | Gynura procumbens polysaccharide with immunological activity and preparation method and application thereof |
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