CN105900809B - A kind of bean sprouts preparation method rich in GABA - Google Patents
A kind of bean sprouts preparation method rich in GABA Download PDFInfo
- Publication number
- CN105900809B CN105900809B CN201610263544.6A CN201610263544A CN105900809B CN 105900809 B CN105900809 B CN 105900809B CN 201610263544 A CN201610263544 A CN 201610263544A CN 105900809 B CN105900809 B CN 105900809B
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- beans
- bean sprouts
- gaba
- bean
- culture solution
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- 244000046052 Phaseolus vulgaris Species 0.000 title claims abstract description 77
- 235000010627 Phaseolus vulgaris Nutrition 0.000 title claims abstract description 77
- BTCSSZJGUNDROE-UHFFFAOYSA-N gamma-aminobutyric acid Chemical compound NCCCC(O)=O BTCSSZJGUNDROE-UHFFFAOYSA-N 0.000 title claims abstract description 29
- 229960003692 gamma aminobutyric acid Drugs 0.000 title claims abstract description 28
- 238000002360 preparation method Methods 0.000 title claims abstract description 11
- 239000011734 sodium Substances 0.000 claims abstract description 9
- 239000001110 calcium chloride Substances 0.000 claims abstract description 8
- 229910001628 calcium chloride Inorganic materials 0.000 claims abstract description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 244000068988 Glycine max Species 0.000 claims description 10
- 235000010469 Glycine max Nutrition 0.000 claims description 10
- 239000000428 dust Substances 0.000 claims description 4
- 239000012535 impurity Substances 0.000 claims description 4
- 229910052708 sodium Inorganic materials 0.000 abstract description 8
- 230000000050 nutritive effect Effects 0.000 abstract description 6
- 102000004190 Enzymes Human genes 0.000 abstract description 4
- 108090000790 Enzymes Proteins 0.000 abstract description 4
- 238000000034 method Methods 0.000 abstract description 4
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 abstract description 3
- 229940124277 aminobutyric acid Drugs 0.000 abstract 1
- 238000012216 screening Methods 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 15
- 230000035784 germination Effects 0.000 description 8
- 240000004922 Vigna radiata Species 0.000 description 7
- 235000010721 Vigna radiata var radiata Nutrition 0.000 description 7
- 235000011469 Vigna radiata var sublobata Nutrition 0.000 description 7
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 3
- 102000008214 Glutamate decarboxylase Human genes 0.000 description 3
- 108091022930 Glutamate decarboxylase Proteins 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- 241000266501 Ormosia ormondii Species 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 235000001014 amino acid Nutrition 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 229910021529 ammonia Inorganic materials 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 230000001766 physiological effect Effects 0.000 description 2
- 235000018102 proteins Nutrition 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- OGNSCSPNOLGXSM-UHFFFAOYSA-N (+/-)-DABA Natural products NCCC(N)C(O)=O OGNSCSPNOLGXSM-UHFFFAOYSA-N 0.000 description 1
- ODHCTXKNWHHXJC-VKHMYHEASA-N 5-oxo-L-proline Chemical compound OC(=O)[C@@H]1CCC(=O)N1 ODHCTXKNWHHXJC-VKHMYHEASA-N 0.000 description 1
- 102000004031 Carboxy-Lyases Human genes 0.000 description 1
- 108090000489 Carboxy-Lyases Proteins 0.000 description 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- IMQLKJBTEOYOSI-GPIVLXJGSA-N Inositol-hexakisphosphate Chemical compound OP(O)(=O)O[C@H]1[C@H](OP(O)(O)=O)[C@@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@@H]1OP(O)(O)=O IMQLKJBTEOYOSI-GPIVLXJGSA-N 0.000 description 1
- IMQLKJBTEOYOSI-UHFFFAOYSA-N Phytic acid Natural products OP(O)(=O)OC1C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C1OP(O)(O)=O IMQLKJBTEOYOSI-UHFFFAOYSA-N 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000000433 anti-nutritional effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000012267 brine Substances 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 210000003169 central nervous system Anatomy 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 229940068041 phytic acid Drugs 0.000 description 1
- 235000002949 phytic acid Nutrition 0.000 description 1
- 239000000467 phytic acid Substances 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- HPALAKNZSZLMCH-UHFFFAOYSA-M sodium;chloride;hydrate Chemical compound O.[Na+].[Cl-] HPALAKNZSZLMCH-UHFFFAOYSA-M 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Cultivation Receptacles Or Flower-Pots, Or Pots For Seedlings (AREA)
- Cultivation Of Plants (AREA)
- Beans For Foods Or Fodder (AREA)
Abstract
The present invention discloses a kind of bean sprouts preparation method rich in GABA.This method is through the following steps that realize: primary election beans, prepares culture solution, cultivates bean sprouts at screening beans.The present invention makes to contain certain density L-sodium (MSG), V in culture solution by adjusting the culture solution of bean germinationCAnd CaCl2, activate the endogenous enzymes of beans, promote GABA(- aminobutyric acid in bean sprouts) enrichment so that the GABA containing high level in the bean sprouts cultivated, further increases the nutritive value and added value of bean sprouts.
Description
Technical field
The invention belongs to food processing technology fields, and in particular to one kind is rich in the bean sprouts system of γ-aminobutyric acid (GABA)
Preparation Method.
Background technique
For beans rich in various nutrient elements such as protein, carbohydrate, nutritive value is especially high, deep to be liked by Chinese people
Love.Beans will be stored in cotyledon breaks down proteins into a variety of amino acid by germination, using the enzyme of itself, while in soybean
The anti-nutritional factors such as phytic acid are degraded, and beany flavor subtracts light, and bioavailability increases significantly, human body absorption easy to digest, promote health.
Especially vitamin content increases significantly, if soybean be free of vitamin C, and in bean sprouts ascorbic content be 20-30 milligrams/
100 grams.Bean sprouts based food is full of nutrition, is a kind of traditional high quality food being favored by people.
GABA is a kind of amino acid for being distributed widely in the intracorporal non-proteinaceous of animals and plants, by Pidolidone through paddy ammonia
Acid decarboxylase catalysis generates, and is the important inhibitory transmitter substance of one kind of central nervous system, has very high physiological activity,
It is played a very important role in the vital movement of animals and plants.It can use its higher enzyme activity during bean germination
To carry out the enrichment of GABA.Traditional bean sprouts when brewed used culture solution be aqueous solution or aqueous salt brine, in germination process
The accumulation ability of GABA is limited, gives full play of the nutritive value of bean sprouts also not.
Summary of the invention
The purpose of the present invention be exactly traditional bean sprouts preparation method there are aiming at the problem that and provide a kind of rich in GABA's
Bean sprouts preparation method.The present invention promotes the enrichment of GABA in bean sprouts, further increases by adjusting the culture solution of bean germination
The nutritive value and added value of bean sprouts.
The purpose of the present invention is what is realized by following technical measures:
Bean sprouts preparation method rich in GABA of the invention, the preparation method is through the following steps that realize:
A, primary election beans: selecting mature and plump and unbroken beans, wash with water, and removes impurity and dust, obtains just
Select beans；
B, it screens beans: 3~6 times of beans volume of water of primary election beans being impregnated into 8-12h, removes dead beans, bad beans, obtains
Completely beans to be steeped；
C, prepare culture solution: the culture solution is made of following component mixing, wherein L-sodium (MSG) 1~4
g/L、VC1~5 g/L, CaCl23~7 mmol/L, surplus are water；
D, cultivate bean sprouts: to prepared culture solution is completely added in beans wait steep, constant temperature is protected from light cultivation, cultivates temperature control
For system at 28~32 DEG C, the cultivation time is 36~72h, and bean sprouts length is 1~3cm.
Heretofore described beans is any one of soya bean or mung bean.
Design philosophy of the invention is as follows:
The present invention makes to contain certain density L-sodium in culture solution by adjusting the culture solution of bean germination
(MSG), VCAnd CaCl2, the endogenous glutamate decarboxylase of beans is activated, the enrichment of GABA in bean sprouts is promoted, so that the beans cultivated
GABA containing higher amount in bud, further increases the nutritive value and added value of bean sprouts.More specifically, GABA is by L- paddy ammonia
Acid is generated through glutamic acid decarboxylase enzymatic, is had very high physiological activity, is played in the vital movement of animals and plants particularly significant
Effect.In the present invention due to using containing L-sodium (MSG), VCAnd CaCl2Training of the aqueous solution as bean germination
Nutrient solution, L-sodium during bean germination in culture solution generate GABA by endogenous glutamic acid decarboxylase enzymatic, greatly
The enrichment of GABA significantly is improved to amplitude, so the content of GABA is than general in the bean sprouts cultivated using the method for the invention
The content of GABA significantly improves in bean sprouts.
Beneficial effects of the present invention are as follows:
The present invention makes to contain certain density L-sodium in culture solution by adjusting the culture solution of bean germination
(MSG), VCAnd CaCl2, activate the endogenous enzymes of beans, promote the enrichment of GABA in bean sprouts so that contain in the bean sprouts cultivated compared with
The GABA of a large amount further increases the nutritive value and added value of bean sprouts.
Specific embodiment
The present invention will be further described in conjunction with the embodiments:
Embodiment 1
300g mature and plump and unbroken soya bean are selected, is washed with water, impurity and dust is removed, obtains primary election soya bean；
5 times of soya bean volume of water of primary election soya bean is impregnated into 12h, removes dead beans, bad beans, obtains completely soya bean to be steeped；By following component
Culture solution is made in mixing, wherein 1 g/L, V of L-sodium (MSG)C 3 g/L、CaCl22.5 mmol/L, surplus are water；To
Prepared culture solution is completely added in soya bean wait steep, 32 DEG C of constant temperature, which are protected from light, cultivates 48h, and yellow bean sprout length is 1~3cm.Gained
Yellow bean sprout after measured, GABA content up to 302.5ug/g(in every gram of bean sprouts), the GABA content than general yellow bean sprout improves
5~8 times.
Embodiment 2
300g mature and plump and unbroken mung bean are selected, is washed with water, impurity and dust is removed, obtains primary election mung bean；
5 times of mung bean volume of water of primary election mung bean is impregnated into 10h, removes dead beans, bad beans, obtains completely mung bean to be steeped；By following component
Culture solution is made in mixing, wherein 3 g/L, V of L-sodium (MSG)C 3 g/L、CaCl25 mmol/L, surplus are water；Xiang Gan
Only prepared culture solution is added in beans wait steep, 32 DEG C of constant temperature, which are protected from light, cultivates 48h, and bean sprouts length is 1~3cm.It is resulting green
After measured, GABA content reaches the bean sprouts 278.3ug/g for bean sprouts, and the GABA content than general mung bean sprouts improves 6~10 times.
Claims (2)
1. a kind of bean sprouts preparation method rich in GABA, it is characterised in that: the preparation method is through the following steps that realize
:
A, primary election beans: selecting mature and plump and unbroken beans, wash with water, and removes impurity and dust, obtains primary election beans
Class；
B, it screens beans: 3~6 times of beans volume of water of primary election beans being impregnated into 8-12h, removes dead beans, bad beans, obtains clean
Beans to be steeped；
C, prepare culture solution: the culture solution is made of following component mixing, wherein 1~4 g/L, V of L-sodiumC1~5
g/L、CaCl23~7 mmol/L, surplus are water；
D, cultivate bean sprouts: to prepared culture solution is completely added in beans wait steep, constant temperature is protected from light cultivation, cultivates temperature control and exists
28~32 DEG C, the cultivation time is 36~72h, and bean sprouts length is 1~3cm.
2. the bean sprouts preparation method according to claim 1 rich in GABA, it is characterised in that: the beans is soya bean or green
Any one of beans.
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| CN201610263544.6A CN105900809B (en) | 2016-04-26 | 2016-04-26 | A kind of bean sprouts preparation method rich in GABA |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610263544.6A CN105900809B (en) | 2016-04-26 | 2016-04-26 | A kind of bean sprouts preparation method rich in GABA |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105900809A CN105900809A (en) | 2016-08-31 |
| CN105900809B true CN105900809B (en) | 2019-04-09 |
Family
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| CN201610263544.6A Expired - Fee Related CN105900809B (en) | 2016-04-26 | 2016-04-26 | A kind of bean sprouts preparation method rich in GABA |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106578967A (en) * | 2016-11-04 | 2017-04-26 | 重庆市农业科学院 | Preparation method of high-activity black bean germ micro powder |
| CN107006606B (en) * | 2017-03-08 | 2020-06-16 | 南京师范大学 | Flavored yogurt rich in functional factors and preparation method thereof |
| CN107258508A (en) * | 2017-06-21 | 2017-10-20 | 磐安县派普特生物科技有限公司 | Bean sprouts soil-less culture liquid |
| CN107616081A (en) * | 2017-10-24 | 2018-01-23 | 贵州省三好食品开发有限公司 | A kind of implantation methods of bean sprouts |
| CN113383911A (en) * | 2021-07-06 | 2021-09-14 | 黑龙江八一农垦大学 | Method for preparing germinated red bean airflow ultramicro powder rich in gamma-aminobutyric acid under stress of vacuum and MSG |
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