CN105876078A - Method for producing biological feed by microbial fermentation of corncobs - Google Patents
Method for producing biological feed by microbial fermentation of corncobs Download PDFInfo
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- CN105876078A CN105876078A CN201410423125.5A CN201410423125A CN105876078A CN 105876078 A CN105876078 A CN 105876078A CN 201410423125 A CN201410423125 A CN 201410423125A CN 105876078 A CN105876078 A CN 105876078A
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Abstract
The invention relates to a method for producing a biological feed by microbial fermentation of corncobs. The method comprises the following main steps: 1, fetching commercially available pediococcus acidilactici, candida utilis, bacillus subtilis, bacillus licheniformis, bifidobacterium and rhodopseudomonas palustris powder, and respectively inoculating the above microbial inoculants to a conventional culture medium to carry out activation culture for 24-48 h so as to obtain an activated bacterial solution; 2, carrying out successive strain amplification on the activated bacterial solution in the conventional culture medium according to the ratio of 1:10-20 for 2-5 times so as to culture a working seed strain solution; 3, preparing a mixed working seed strain solution; 4, respectively dissolving an enzymic preparation in the above mixed working seed strain solution to prepare a biological fermentation solution; 5, spraying the biological fermentation solution onto the surface of corncobs which have been crushed and immersed with clean water for 24-48 h according to the ratio of 500-1000g of the biological fermentation solution to each ton of corncobs, stirring, and controlling water content to 65-75%; and 6, fermenting at the humidity of 50-80% and at the air temperature of 20-36 DEG C for 5-7 days so as to produce the biological feed.
Description
Technical field
The present invention relates to a kind of method utilizing fermentable corn cob to produce biological feedstuff, belong to microorganism
Fermentation technical field.
Background technology
Semen Maydis is the second largest cereal crops that Guangxi is only second to Oryza sativa L., and annual production reaches 2,700,000 t, corresponding corn cob
Yield estimate also at 90,000 t, quantity is the most considerable.The most substantial amounts of corn cob except part be used as furfural,
Outside the raw material of the products such as xylitol, greatly by as agricultural wastes, direct burn processing, causes
The significant wastage of resource, again to environment.Corn cob contains abundant nutrient substance, crude protein 1.1%,
Crude fat 0.6%, crude fibre 31.8%, soluble saccharide 51.8%, possibly together with calcium, phosphorus, magnesium, ferrum, potassium etc.
Multi mineral prime element.But its crude fiber content is high, so being directly used as the feedstuff palatability difference of poultry and disappearing
Change utilization rate the highest, apply less in Animal husbandry production.
Guangxi is also the province of a feedstuff (especially albumen feedstuff) critical shortage, needs import big every year
The fish meal protein of amount, with tonifying for the deficiency, the most annual feed-use grain about 1,000,000 tons, defines extremely serious
" people and animals earn grain " situation, in the urgent need to opening up the new albumen feedstuff energy.And if able to make full use of rich
Rich corn cob produces albumen feedstuff, not only eliminates the corn cob pollution to environment, improves the warp of breeding enterprise
Ji benefit, and can alleviate the wretched insufficiency of albumen feedstuff and people and animals earn the situation of grain, animal husbandry is had
Important realistic meaning.
Summary of the invention
It is an object of the invention to provide a kind of method utilizing fermentable corn cob to produce biological feedstuff.
This method can efficiently, quickly and conveniently to corn cob be fermented, and has open.Without detesting
Oxygen environment, to features such as temperature requirement are low, overcoming existing corn cob fermentation technique needs anaerobic condition, raw
Produce the deficiency that cost is high.
It is an object of the invention to be accomplished by, it is raw that one utilizes fermentable corn cob to produce
The method of thing feedstuff, key step is as follows:
(1) take commercially available pediococcus acidilactici, Candida utilis, bacillus subtilis, Bacillus licheniformis,
Bacillus bifidus, Rhodopseudomonas palustris mycopowder, be inoculated in respectively in conventional medium and carry out actication of culture, lives
Change time 24～48 hours, obtain activating bacterium solution；
(2) take the above-mentioned activation bacterium solution ratio with 1: 10～20 in conventional medium respectively and carry out 2～5 times
The amplification of gradually strain quantity, cultivate as work seed strain liquid under 25～36 DEG C of environment；
(3) above-mentioned pediococcus acidilactici, Candida utilis, bacillus subtilis, lichens spore bar are taken respectively
Bacterium, bacillus bifidus, Rhodopseudomonas palustris work seed strain liquid with 6～12: 5～12: 5～12: 5～
The proportions of 8: 4～12: 5～12 becomes hybrid working seed strain liquid, makes in hybrid working seed strain liquid total
Number of viable reaches 100～20,000,000,000 cfu/ml；
(4) difference extracting cellulose enzyme, hemicellulase, non-starch polysaccharide enzyme, protease and diastase
Any one of or several ratio in 12%～the 20% of hybrid working seed strain liquid weight be dissolved in above-mentioned mixed
Close in work seed strain liquid and be fabricated to bio-fermented liquid；
(5) the above-mentioned bio-fermented liquid prepared is sprayed onto by the consumption of corn cob 500～1000g per ton
Size-reduced and soak with clean water 24～48h the surface of corn cob, stirring, make bio-fermentation agent and Semen Maydis
Core uniformly mixes, and controls water content between 65～75%；
(6) after above-mentioned bio-fermentation agent and corn cob mixing, in humidity 50%～80%, temperature 20 DEG C～36
In the range of DEG C, ferment and i.e. produce to obtain biological feedstuff in 5～7 days.
Taking the present invention of above-mentioned measure, the bio-fermented liquid of preparation has conversion decomposition to corn cob,
Improve the palatability of corn cob feedstuff, and corn cob bioavailability in animal can be improved.This
Bright described probiotic bacteria can increase immunity and the premunition of animal, reduces animal morbidity, reduces and even dispel
Deodorization gas, improves breeding environment.Preparation cost and use cost ratio are relatively low, process used by 1 ton of corn cob
Microbial bacterial agent cost about 3 yuan.Easy and simple to handle, dissemination is very big.
Detailed description of the invention
Embodiment 1
(1) take commercially available pediococcus acidilactici, Candida utilis, bacillus subtilis, Bacillus licheniformis,
Bacillus bifidus, Rhodopseudomonas palustris mycopowder, be inoculated in respectively in conventional medium and carry out actication of culture, lives
48 hours change time, obtain activating bacterium solution；
(2) take the above-mentioned activation bacterium solution ratio with 1: 10～20 in conventional medium respectively and carry out 5 times
Gradually strain quantity amplification, cultivates as work seed strain liquid under 25～36 DEG C of environment；
(3) above-mentioned pediococcus acidilactici, Candida utilis, bacillus subtilis, lichens spore bar are taken respectively
Bacterium, bacillus bifidus, Rhodopseudomonas palustris work seed strain liquid with 12: 9: 8: the proportions of 5: 8: 9
Become hybrid working seed strain liquid, make total number of viable in hybrid working seed strain liquid reach 15,000,000,000
cfu/ml；
(4) hybrid working seed strain liquid weight pressed by extracting cellulose enzyme, protease and diastase respectively
The ratio of 12% is dissolved in above-mentioned hybrid working seed strain liquid and is fabricated to bio-fermented liquid；
(5) the above-mentioned bio-fermented liquid prepared is sprayed onto size-reduced by the consumption of corn cob 600g per ton
And the surface of the corn cob with clean water immersion 24h, stirring, make bio-fermentation agent and corn cob uniformly mix,
Control water content between 65～75%；
(6) after above-mentioned bio-fermentation agent and corn cob mixing, in humidity 50%～80%, temperature 20 DEG C～36
In the range of DEG C, ferment and i.e. produce to obtain biological feedstuff in 5～7 days.
Embodiment 2
(1) take commercially available pediococcus acidilactici, Candida utilis, bacillus subtilis, Bacillus licheniformis,
Bacillus bifidus, Rhodopseudomonas palustris mycopowder, be inoculated in respectively in conventional medium and carry out actication of culture, lives
24 hours change time, obtain activating bacterium solution；
(2) take the above-mentioned activation bacterium solution ratio with 1: 10～20 in conventional medium respectively and carry out 3 times
Gradually strain quantity amplification, cultivates as work seed strain liquid under 25～36 DEG C of environment；
(3) above-mentioned pediococcus acidilactici, Candida utilis, bacillus subtilis, lichens spore bar are taken respectively
Bacterium, bacillus bifidus, Rhodopseudomonas palustris work seed strain liquid with 12: 12: 5: the ratio of 5: 9: 8 is joined
Make hybrid working seed strain liquid, make total number of viable in hybrid working seed strain liquid reach 20,000,000,000
cfu/ml；
(4) hybrid working seed strain liquid pressed by extracting cellulose enzyme, non-starch polysaccharide enzyme and diastase respectively
The ratio of the 20% of weight is dissolved in above-mentioned hybrid working seed strain liquid and is fabricated to bio-fermented liquid；
(5) the above-mentioned bio-fermented liquid prepared is sprayed onto size-reduced by the consumption of corn cob 500g per ton
And the surface of the corn cob with clean water immersion 48h, stirring, make bio-fermentation agent and corn cob uniformly mix,
Control water content between 65～75%；
(6) after above-mentioned bio-fermentation agent and corn cob mixing, in humidity 50%～80%, temperature 20 DEG C～36
In the range of DEG C, ferment and i.e. produce to obtain biological feedstuff in 5～7 days.
Embodiment 3
(1) take commercially available pediococcus acidilactici, Candida utilis, bacillus subtilis, Bacillus licheniformis,
Bacillus bifidus, Rhodopseudomonas palustris mycopowder, be inoculated in respectively in conventional medium and carry out actication of culture, lives
36 hours change time, obtain activating bacterium solution；
(2) take the above-mentioned activation bacterium solution ratio with 1: 10～20 in conventional medium respectively and carry out 4 times
Gradually strain quantity amplification, cultivates as work seed strain liquid under 25～36 DEG C of environment；
(3) above-mentioned pediococcus acidilactici, Candida utilis, bacillus subtilis, lichens spore bar are taken respectively
Bacterium, bacillus bifidus, Rhodopseudomonas palustris work seed strain liquid with 6: 5: 8: the ratio of 7: 12: 12 is joined
Make hybrid working seed strain liquid, make total number of viable in hybrid working seed strain liquid reach 10,000,000,000
cfu/ml；
(4) difference extracting cellulose enzyme, hemicellulase, non-starch polysaccharide enzyme, protease and diastase
System it is dissolved in above-mentioned hybrid working seed strain liquid in the ratio of the 16% of hybrid working seed strain liquid weight
It is made bio-fermented liquid；
(5) the above-mentioned bio-fermented liquid prepared is sprayed onto size-reduced by the consumption of corn cob 800g per ton
And the surface of the corn cob with clean water immersion 36h, stirring, make bio-fermentation agent and corn cob uniformly mix,
Control water content between 65～75%；
(6) after above-mentioned bio-fermentation agent and corn cob mixing, in humidity 50%～80%, temperature 20 DEG C～36
In the range of DEG C, ferment and i.e. produce to obtain biological feedstuff in 5～7 days.
Claims (2)
1. the method utilizing fermentable corn cob to produce biological feedstuff, is characterized in that described production is raw
The key step of thing feedstuff method:
(1) take commercially available pediococcus acidilactici, Candida utilis, bacillus subtilis, Bacillus licheniformis,
Bacillus bifidus, Rhodopseudomonas palustris mycopowder, be inoculated in respectively in conventional medium and carry out actication of culture, lives
Change time 24～48 hours, obtain activating bacterium solution；
(2) take the above-mentioned activation bacterium solution ratio with 1: 10～20 in conventional medium respectively and carry out 2～5 times
The amplification of gradually strain quantity, cultivate as work seed strain liquid under 25～36 DEG C of environment；
(3) above-mentioned pediococcus acidilactici, Candida utilis, bacillus subtilis, lichens spore bar are taken respectively
Bacterium, bacillus bifidus, Rhodopseudomonas palustris work seed strain liquid with 6～12: 5～12: 5～12: 5～
The proportions of 8: 4～12: 5～12 becomes hybrid working seed strain liquid, makes in hybrid working seed strain liquid
Total number of viable reaches 100～20,000,000,000 cfu/ml；
(4) difference extracting cellulose enzyme, hemicellulase, non-starch polysaccharide enzyme, protease and diastase
Any one of or several ratio in 12%～the 20% of hybrid working seed strain liquid weight be dissolved in above-mentioned mixed
Close in work seed strain liquid and be fabricated to bio-fermented liquid；
(5) the above-mentioned bio-fermented liquid prepared is sprayed onto by the consumption of corn cob 500～1000g per ton
Size-reduced and soak with clean water 24～48h the surface of corn cob, stirring, make bio-fermentation agent and Semen Maydis
Core uniformly mixes, and controls water content between 65～75%；
(6) after above-mentioned bio-fermentation agent and corn cob mixing, in humidity 50%～80%, temperature 20 DEG C～36
In the range of DEG C, ferment and i.e. produce to obtain biological feedstuff in 5～7 days.
2. the method that the fermentable corn cob as described in step 4 in claim 1 produces biological feedstuff,
It is characterized in that: cellulase, hemicellulase, non-starch polysaccharide enzyme, protease and diastase are
Solid-state version, enzyme 2850U/g～3000U/g, 2500U/g～the 2600U/g of being respectively, 2700U/g～2950U/g alive,
3500U/g～3750U/g, 3700U/g～3950U/g.
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| CN201410423125.5A CN105876078A (en) | 2014-08-26 | 2014-08-26 | Method for producing biological feed by microbial fermentation of corncobs |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109105642A (en) * | 2018-09-07 | 2019-01-01 | 内蒙古农业大学 | A kind of antioxidant feed additive and its preparation method and application rich in polysaccharide that fermented maize core obtains |
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| CN102125163A (en) * | 2010-12-31 | 2011-07-20 | 宜春强微生物科技有限公司 | Feed ferment and method for producing fermented feed by utilizing same |
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| CN102488120A (en) * | 2011-12-28 | 2012-06-13 | 广西东园生态农业科技有限公司 | Preparation method of fermenting ensilaged feed |
| CN102715350A (en) * | 2012-06-18 | 2012-10-10 | 南开大学 | Production method of straw feed |
| CN103598409A (en) * | 2013-10-29 | 2014-02-26 | 界首市春秋牧业有限公司 | Method for preparation of feed by fermenting corncobs |
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2014
- 2014-08-26 CN CN201410423125.5A patent/CN105876078A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102125163A (en) * | 2010-12-31 | 2011-07-20 | 宜春强微生物科技有限公司 | Feed ferment and method for producing fermented feed by utilizing same |
| CN102125169A (en) * | 2011-01-06 | 2011-07-20 | 宜春强微生物科技有限公司 | Dreg feed leaven and application thereof |
| CN102488120A (en) * | 2011-12-28 | 2012-06-13 | 广西东园生态农业科技有限公司 | Preparation method of fermenting ensilaged feed |
| CN102715350A (en) * | 2012-06-18 | 2012-10-10 | 南开大学 | Production method of straw feed |
| CN103598409A (en) * | 2013-10-29 | 2014-02-26 | 界首市春秋牧业有限公司 | Method for preparation of feed by fermenting corncobs |
Non-Patent Citations (1)
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109105642A (en) * | 2018-09-07 | 2019-01-01 | 内蒙古农业大学 | A kind of antioxidant feed additive and its preparation method and application rich in polysaccharide that fermented maize core obtains |
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