The present invention's purpose aims to provide a kind of can be used for food, cosmetics, health product, Nicotiana tabacum L. etc., the carcinogenesis that extensively is present in the multiple carcinogen in the human habitat is had galenical of inhibition and antagonism and preparation method thereof.
Be equivalent to Seem Lablab Album 100-150g, Radix Glycyrrhizae 25-30g, Flos Lonicerae 20-25g, Radix Linderae 20-25g, dry Fructus Crataegi slice 20-25g, Herba Cirsii 20-30g in every 1000g preparation of the present invention.Described Seem Lablab Album, Radix Glycyrrhizae, Flos Lonicerae, the Radix Linderae are the concentrated solutions of their water logging extracting solution, and described dry Fructus Crataegi slice, Herba Cirsii are the sweet dry distilling volatilization of soaking of they refrigerants.
Product of the present invention is prepared by the following method: press Seem Lablab Album 100-150g, Radix Glycyrrhizae 25-30g, Flos Lonicerae 20-25g, Radix Linderae 20-25g, dry Fructus Crataegi slice 20-25g, the ratio of Herba Cirsii 20-30g is got raw material ready, press Mel 30%, the ratio of distilled water 70% prepares soaks sweet liquid and heated constant temperature at 40-45 ℃, put into dry Fructus Crataegi slice, Herba Cirsii is soaked honey and handled 6-10 hour, to through soak honey dry Fructus Crataegi slice, Herba Cirsii carries out dry distilling and collects its volatilization refrigerant, with Seem Lablab Album, Radix Glycyrrhizae, Flos Lonicerae, the water of 10 times of weight of Radix Linderae adding heats up after soaking 8-12 hour under the 65-70 ℃ of temperature again and boiled 1-4 hour, get its water extract and dry Fructus Crataegi slice, the soaking to concentrate after the sweet dry distilling volatilization refrigerant phase mixing and stirring of Herba Cirsii made the 1000g preparation.
Product of the present invention is that primary raw material is made with six kind of plant.Radix Glycyrrhizae contains glycyrrhizin, and various poisonous and harmful compositions are had adsorption, and Fructus Crataegi contains epicatechin, Pi Su, chlorogenic acid, and cachou extract, chlorogenic acid are the natural anti-cancer compositions; Seem Lablab Album contains sucrose, glucose, maltose, L-pipecolic acid and phytohemagglutinin, and glucose, maltose have detoxifcation and the active effect of anti-harmful organism, and especially phytohemagglutinin has the stronger effect that is adsorbed with harmful substances; Flos Lonicerae contains luteolin, chlorogenic acid, isochlorogenic acid etc., and chlorogenic acid and isochlorogenic acid are the natural anti-cancer materials; It is to suppress the active composition of harmful organism that Herba Cirsii contains protocatechuic acid, caffeic acid, chlorogenic acid etc.; The Radix Linderae contains multiple Xinfeng terpenoid composition.Various compositions are after method processing of the present invention is refined, and coordination cooperatively interacts.In conjunction with or absorption mutation and mutagen matter, act on body cell cytochrome p 450 mixing mixed-functional oxidase, stoping has active carcinogen to produce, act on glutathion and separate toxogenin, strengthen the function of detoxification of body, promote the effect of body DNA damage repair system, the short nucleophilic site of protecting body DNA does not combine carcinogen with DNA.
Product of the present invention is pure galenical, nontoxic, through acute toxicity testing LD50 all greater than the 20g/Kg body weight, non-toxic reaction as a result, international Salmonella typhimurium/mammal microsomal enzyme experiment (AMES test) according to national GB15193.1-94 regulation, the result shows does not have mutagenesis (carcinogenic disease) effect in dosage is 0.82mg/ ware-103mg/ ware scope, the mouse bone marrow cells micronucleus test, the result shows, at dosage is 5g/kg-20g/kg weight range the interior of the body belonging to YIN result, and the mouse sperm deformity result of the test shows in 5g/kg-20g/kg weight range the interior of the body belonging to YIN result.Above-mentioned toxicological experiment result shows that product of the present invention belongs to nontoxic safe preparation according to the regulation of standard GB 15132.1-94.The additive of food, cosmetics, health product be can be used as, anti-cancer, anticancer effect played.In being 4.8mg/ ware-120mg/ ware scope, can suppress dosage among the human lives the extensively strong carcinogen 3 of contact fully, the carcinogenesis of 4-stupid also (a) pyrene and have tangible dosage and reaction relation; In dosage is 4.8mg/ ware-120mg/ ware scope, can suppress to contain the carcinogenesis of the cigarette coke tar of multiple carcinogen fully, have certain dose-response relationship; Simultaneously; product of the present invention is sprayed on the cigarette shreds with the dosage of 0.5% (with the tobacco shred weight ratio); normal combustion through medicated cigarette; can reduce the carcinogenesis of coke tar in cigarette significantly; the acute toxicity that can the utmost point reduces smoke from cigarette significantly; protect the healthy of smoker and involuntary smoker effectively, and do not change the main chemical of Nicotiana tabacum L..Coordinating with the fragrance flavor of Nicotiana tabacum L., reduce zest, increase pure and mild degree, certain flavouring effect is arranged, is a kind of good Safety cigarette additive.
For example the present invention is done more detailed description below:
Get dry Fructus Crataegi slice 20g, Herba Cirsii 20g, press Mel 30%, the weight ratio of distilled water 70% prepares soaks sweet liquid, to soak sweet liquid and be heated to 40-45 ℃ and keep constant temperature, put into dry Fructus Crataegi slice again, Herba Cirsii is soaked honey and handled 8 hours, allow and soak sweet liquid and fully suck in Fructus Crataegi slice mountain and the Herba Cirsii, pull out and put into distillation apparatus and carry out dry distilling, its volatile matter is through cooling, collect the volatilization refrigerant; get Seem Lablab Album 100g; Radix Glycyrrhizae 25g; Flos Lonicerae 20g; the water of Radix Linderae 20g adding 10 times (weight) heats up again and boiled 3 hours; get its water extracting liquid after soaking 8-12 hour under the 65-70 ℃ of constant temperature; with above-mentioned prepared volatilization refrigerant of two steps; water extracting liquid mixes; stir, reconcentration becomes the 1000g product.
Another embodiment of the present invention is to get dry Fructus Crataegi slice 25g, Herba Cirsii 30g.Prepare by the weight ratio of Mel 30%, distilled water 70% and to soak sweet liquid, to soak sweet liquid and be heated to 40-45 ℃ and keep constant temperature, putting into dry Fructus Crataegi slice, Herba Cirsii again soaks honey and handled 8 hours, allow and soak sweet liquid and fully suck in Fructus Crataegi slice and the Herba Cirsii, pull out and put into distillation apparatus and carry out dry distilling, its volatile matter is collected the volatilization refrigerant through cooling; get Seem Lablab Album 150g, Radix Glycyrrhizae 30g, Flos Lonicerae 25g, Radix Linderae 25g add water extraction, in order to improve extraction ratio.Water is carried three steps of branch and is carried out, the water that first step adding is 5 times soaked 8-12 hour under 65-70 ℃ of temperature, heat up then and boiled 1.5 hours, take out the decocting in water concentrated solution, the water boil that the second step adding is 4 times 1 hour takes out the decocting in water concentrated solution, the water boil that the 3rd step adding is 3 times 0.5 hour, take out the decocting in water concentrated solution, three times decocting in water concentrated solutions are mixed.