CN105861366A - Application of polycyclic aromatic hydrocarbon degrading strain FA1 in absorption of heavy metal Pb ions - Google Patents
Application of polycyclic aromatic hydrocarbon degrading strain FA1 in absorption of heavy metal Pb ions Download PDFInfo
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- 229910001385 heavy metal Inorganic materials 0.000 title claims abstract description 40
- 125000005575 polycyclic aromatic hydrocarbon group Chemical group 0.000 title claims abstract description 19
- 238000010521 absorption reaction Methods 0.000 title abstract description 15
- 150000002500 ions Chemical class 0.000 title abstract description 11
- 230000000593 degrading effect Effects 0.000 title abstract description 7
- 244000005700 microbiome Species 0.000 claims abstract description 18
- 241001538178 Herbaspirillum chlorophenolicum Species 0.000 claims abstract description 11
- 238000004321 preservation Methods 0.000 claims abstract description 8
- 238000001179 sorption measurement Methods 0.000 claims description 46
- 241000894006 Bacteria Species 0.000 claims description 25
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 12
- 239000003463 adsorbent Substances 0.000 claims description 9
- 230000015556 catabolic process Effects 0.000 claims description 7
- 238000006731 degradation reaction Methods 0.000 claims description 7
- 238000000034 method Methods 0.000 claims description 7
- 239000007640 basal medium Substances 0.000 claims description 6
- 239000002131 composite material Substances 0.000 claims description 6
- 239000011780 sodium chloride Substances 0.000 claims description 6
- 238000006243 chemical reaction Methods 0.000 claims description 5
- 239000001888 Peptone Substances 0.000 claims description 2
- 108010080698 Peptones Proteins 0.000 claims description 2
- 241000022165 bacterium FA1 Species 0.000 claims description 2
- 239000006071 cream Substances 0.000 claims description 2
- 239000008367 deionised water Substances 0.000 claims description 2
- 229910021641 deionized water Inorganic materials 0.000 claims description 2
- 235000019319 peptone Nutrition 0.000 claims description 2
- 238000005406 washing Methods 0.000 claims description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 2
- 235000015278 beef Nutrition 0.000 claims 1
- 238000007710 freezing Methods 0.000 claims 1
- 230000008014 freezing Effects 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 abstract description 3
- 239000000463 material Substances 0.000 abstract 1
- 238000009629 microbiological culture Methods 0.000 abstract 1
- 230000001580 bacterial effect Effects 0.000 description 29
- RVPVRDXYQKGNMQ-UHFFFAOYSA-N lead(2+) Chemical compound [Pb+2] RVPVRDXYQKGNMQ-UHFFFAOYSA-N 0.000 description 26
- 238000002474 experimental method Methods 0.000 description 10
- 230000000694 effects Effects 0.000 description 5
- 238000012512 characterization method Methods 0.000 description 4
- GVEPBJHOBDJJJI-UHFFFAOYSA-N fluoranthene Chemical compound C1=CC(C2=CC=CC=C22)=C3C2=CC=CC3=C1 GVEPBJHOBDJJJI-UHFFFAOYSA-N 0.000 description 4
- 238000005070 sampling Methods 0.000 description 4
- 230000000844 anti-bacterial effect Effects 0.000 description 3
- 238000006065 biodegradation reaction Methods 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 229910052751 metal Inorganic materials 0.000 description 3
- 239000002184 metal Substances 0.000 description 3
- 239000002028 Biomass Substances 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000003673 groundwater Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 239000010802 sludge Substances 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- 241000195493 Cryptophyta Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000605016 Herbaspirillum Species 0.000 description 1
- 239000002250 absorbent Substances 0.000 description 1
- 230000002745 absorbent Effects 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 231100000704 bioconcentration Toxicity 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000009514 concussion Effects 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- -1 hydrogen ions Chemical class 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 230000002262 irrigation Effects 0.000 description 1
- 238000003973 irrigation Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000005065 mining Methods 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000007096 poisonous effect Effects 0.000 description 1
- 229950000845 politef Drugs 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 238000005067 remediation Methods 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 239000002356 single layer Substances 0.000 description 1
- 239000002910 solid waste Substances 0.000 description 1
- 239000002594 sorbent Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 239000002912 waste gas Substances 0.000 description 1
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/32—Hydrocarbons, e.g. oil
- C02F2101/327—Polyaromatic Hydrocarbons [PAH's]
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Abstract
The invention discloses application of a polycyclic aromatic hydrocarbon degrading strain FA1 in absorption of heavy metal Pb ions. The category of the polycyclic aromatic hydrocarbon degrading strain FA1 is named as herbaspirillum chlorophenolicum; the polycyclic aromatic hydrocarbon degrading strain FA1 is preserved in China General Microbiological Culture Collection Center (CGMCC) in May 4th, 2010, the preservation address is No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No. 3797. The invention provides the strain and gene material for repairing microorganisms which have heavy metal and polycyclic aromatic hydrocarbon combined pollution to the environment.
Description
Technical field
Present invention relates particularly to polycyclic aromatic hydrocarbon-degrading bacteria FA1 application in Adsorption of Heavy Metals Pb ion.
Background technology
Due to the factors such as mining, waste gas discharge, solid waste accumulation and sewage irrigation, a large amount of poisonous and harmful weights
Metal enters in environment and continues to build up.Heavy metal contaminants is degraded the most hardly, and along with food
Thing chain and be enriched with, there is the strongest bioconcentration so that ecological environment and human health face serious threat.
As one of heavy metal that toxicity is maximum, lead concentration over-standard phenomenon in China's soil and groundwater environment is frequent
Be seen in report.In recent years, heavy metal pollution with lead as representative causes environment and social problem are increasingly
Prominent, therefore, the reparation of Heavy Metals In Environment is the most singly one of the study hotspot in current environment protection field,
Have more important realistic meaning.
Restorative procedure currently for heavy metal mainly has peripheral doses, chemical redemption and biological restoration etc..Biological
The features such as restorative procedure has efficiently, low consumption, economy, environmental friendliness and easily operated management, than traditional
Physics, chemical restoration have clear superiority, in particular for the heavy metal pollution situation of low concentration.This skill
It is critical only that of art filters out the microorganism with stronger Adsorption of Heavy Metals ability.Can micro-life of Adsorption of Heavy Metals
Thing includes antibacterial, fungus and algae etc., and wherein, antibacterial is the most excellent due to its strongest biochemical adaptability
Gesture.
It is worthy of note, the heavy metal biological of existing report is repaired mostly for single heavy metal pollution feelings
Shape, but the heavy metal pollution in environment often occurs with other organic pollutions simultaneously.As heavy metal-organic
The Typical Representative of thing combined pollution, heavy metal and polycyclic aromatic hydrocarbon composite pollution phenomenon are in China's soil and groundwater
It is widely present.When two kinds of pollutant coexist, the toxic effect of polycyclic aromatic hydrocarbon is by can the microorganism of Adsorption of Heavy Metals
Cause and coerce, harm the adsorption to its heavy metal.Therefore, for the heavy metal in actual environment-many
PAH combined pollution present situation, it is necessary to filtering out resistance to polycyclic aromatic hydrocarbon toxicity even can the weight of degrading polycyclic aromatic hydrocarbons
Metal adsorption microorganism, studies its characterization of adsorption, for heavy metal and the microorganism of polycyclic aromatic hydrocarbon composite pollution environment
Repair and bacterial strain and genetic material are provided.
Summary of the invention
By investigating the adsorptivity of the polycyclic aromatic hydrocarbon-degrading bacteria heavy metal Pb ion of the existing different genera of seminar
Can, present invention discover that studying polycyclic aromatic hydrocarbon-degrading bacteria FA1 heavy metal Pb filtered out before has good
Toleration and adsorptivity.The named Herbaspirillum of class of this polycyclic aromatic hydrocarbon-degrading bacteria FA1
Chlorophenolicum, is preserved in China Committee for Culture Collection of Microorganisms on May 4th, 2010
Common micro-organisms center, preservation address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, and preserving number is
CGMCC No.3797.Screening technique and the bacterial strain performance of this polycyclic aromatic hydrocarbon-degrading bacteria FA1 are shown in seminar XU
Deng article " the Degradation of fluoranthene by a newly isolated strain of delivered for 2011
Herbaspirillum chlorophenolicum from activated sludge ", Biodegradation, 22 (2),
335-345。
In view of it is for the adsorption of heavy metal Pb ion, present invention further proposes above-mentioned polycyclic aromatic hydrocarbon
The application in administering heavy metal-polycyclic aromatic hydrocarbon composite pollution of degradation bacteria FA1.
Further, the invention allows for above-mentioned polycyclic aromatic hydrocarbon-degrading bacteria FA1 and prepare resistance to polycyclic aromatic hydrocarbon
Adsorbent for heavy metal in application, described adsorbent is prepared via a method which to obtain: by polycyclic aromatic hydrocarbon
Degradation bacteria FA1 accesses in basal medium and carries out enrichment culture, then by be in the bacterium solution of exponential phase with
8000rpm frozen centrifugation 10min, the NaCl solution washing with 100～150mM/L 3 times, 4 DEG C of cold preservations are standby
With.
Preferably, described basal medium comprises Carnis Bovis seu Bubali cream 0.5%, peptone 1.0% and NaCl 0.5%,
Surplus is deionized water, pH7.0～7.2.
In above-mentioned application, when for Adsorption of Heavy Metal Ions, for ensureing adsorption effect, reactant should be regulated
Be temperature be 20～35 DEG C, pH is 2～6.
It is highly preferred that when for Adsorption of Heavy Metal Ions, regulation temperature of reaction system is 25～30 DEG C, pH
It is 5～6.Most preferably, when for Adsorption of Heavy Metal Ions, regulation temperature of reaction system is 30 DEG C, pH
It is 5.5.
Beneficial effect: the present invention have unexpectedly discovered that can simultaneously degrading polycyclic aromatic hydrocarbons and heavy metal ion multi-ring
Biodegradation of PAHs bacterium Herbaspirillum chlorophenolicum FA1, and by studying its characterization of adsorption, attach most importance to
The microorganism remediation of metal and polycyclic aromatic hydrocarbon composite pollution environment provides bacterial strain and genetic material.
Accompanying drawing explanation
Fig. 1 is that bacterial strain FA1 is at variable concentrations Pb2+Growth curve in solution；
Fig. 2 is that bacterial strain FA1 is to Pb2+Adsorption dynamics adsorption kinetics；
Fig. 3 is that bacterial strain FA1 is to Pb2+Adsorption isotherm；
Fig. 4 is that bacterial strain FA1 is adsorbed Pb by temperature2+The impact of efficiency；
Fig. 5 is that bacterial strain FA1 is adsorbed Pb by pH2+The impact of efficiency.
Detailed description of the invention
The present invention be may be better understood according to following embodiment.But, those skilled in the art is easy to understand
, the content described by embodiment is merely to illustrate the present invention, and should be also without limitation on claims
The present invention described in detail by.
The preparation of embodiment 1 heavy metal absorbent.
The present embodiment microorganism used therefor is from the polycyclic aromatic hydrocarbon-degrading bacteria filtered out before seminar
Herbaspirillum chlorophenolicum FA1 (Xu et al.2011, Degradation of fluoranthene
by a newly isolated strain of Herbaspirillum chlorophenolicum from activated
Sludge ", Biodegradation, 22 (2), 335-345).Bacterial strain FA1 is in the GenBank data of NCBI
Sequence accession number in storehouse is HM107777, is preserved in Chinese microorganism strain on May 4th, 2010
Preservation administration committee common micro-organisms center, preservation address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3
Number, Institute of Microorganism, Academia Sinica, register on the books numbered CGMCC No.3797.
Sorbent preparation method: before inoculation, accesses bacterial strain FA1 in basal medium and carries out enrichment culture, so
After by being in the bacterium solution of exponential phase with 8000rpm frozen centrifugation 10min, with the NaCl solution of 150mM/L
Washing 3 times, obtain adsorbent, 4 DEG C of cold preservations are standby.
Embodiment 2 microorganism heavy metal Pb2+Characterization of adsorption research.
All experimental grouies of following characterization of adsorption research are equipped with 3 parallel and blank groups.
(1) microorganism heavy metal Pb2+Toleration
Take Pb (NO3)2, add basal medium so that Pb2+Concentration is respectively 0,10,20,50,100,
200mg/L, and to regulate pH value of solution be 5.5, inoculates into bacterial strain FA1, in concussion incubator 30 DEG C, 125rpm
Constant temperature culture, timing sampling, the OD of solution is measured with ultraviolet spectrophotometer600Value, draws with the time as horizontal stroke
The growth curve of coordinate.
Polycyclic aromatic hydrocarbon-degrading bacteria FA1 is at variable concentrations Pb2+Growth curve under existence condition is as shown in Figure 1.
With without Pb2+Comparison compare, as Pb in culture medium2+When concentration is 10mg/L, the growth of bacterial strain FA1
It is barely affected.Along with Pb2+The further increase of concentration, the speed of growth of bacterial strain gradually slows down, Biomass
Corresponding OD600Value diminishes the most therewith, and Pb is described2+The growth and breeding of bacterial strain there is certain inhibitory action.But
It is to it is worthy of note, the Pb of higher concentration2+Concentration (20-200mg/L) does not delay bacterial strain to enter
Exponential phase.At Pb2+During at concentrations up to 200mg/L, it is the Biomass of (15h) in exponential phase
Compared to without Pb2+For the matched group of concentration, only only reduce 28.3%, show Herbaspirillum
Chlorophenolicum FA1 heavy metal lead has good toleration, has the value of research further.
(2) adsorption dynamics adsorption kinetics
Adsorbent prepared by embodiment 1 is accessed to the Pb (NO containing 30mg/L3)2The politef of solution
In centrifuge tube, the access concentration of adsorbent is 60mg/L, sets in background solution the concentration of NaCl as 150
MM/L, the initial pH of regulation system are 5.5.Adsorption reaction system is placed in isothermal vibration incubator, arranges
Rotating speed is 125rpm, and temperature is 30 DEG C, samples at regular intervals, filter membrane (0.22 μm), uses atom
Absorption spectrophotometer (AAS) measures Pb remaining in solution2+Concentration.
Polycyclic aromatic hydrocarbon-degrading bacteria FA1 Adsorption of Heavy Metals lead is had a major impact by the time of contact of cell and lead ion.
Using its adsorption dynamics adsorption kinetics of batch Research on experimental methods, result is as shown in Figure 2.At the absorption initial stage, bacterial strain is to molten
In liquid, the absorption of lead ion is more rapid, and 5h adsorbance i.e. reaches during adsorption equilibrium total adsorbance more than half,
Adsorbance increase afterwards slows down, and the rate of adsorption declines.After 25h, adsorbance reaches total adsorbance during adsorption equilibrium
80%, bacterial strain is to Pb2+Absorption after 80h, reach complete equipilibrium.
To Pb in bacterial strain FA1 adsorbent solution2+Dynamics data be analyzed matching, find pseudo-second order kinetic
With Elovich kinetic model, the fitting effect of experimental result is compared other models preferably, the phase relation of matching
Number R2The highest, respectively 0.986 and 0.9643.Meet pseudo-second order kinetic relation table detail bacterium FA1 pair
Pb in solution2+Absorption be exchanged for main with chemisorbed or ion, and Elovich model is to experimental result matching
Good then explanation Herbaspirillum chlorophenolicum FA1 to Pb2+Biological adsorption be one non-all
The diffusion process of phase.
(3) adsorption isotherm experiment
Initial p b of series is set2+Concentration is 5,10,20,30,50,100,200mg/L, adsorbs power in 2
The experiment condition learned carries out the adsorption isotherm experiment at 30 DEG C, and after absorption reaches balance, the balance of sampling and measuring lead is dense
Degree and adsorbance.
At 30 DEG C, bacterial strain FA1 (dosage 60mg/L) Pb in solution (pH=5.5)2+(the denseest
Degree 30mg/L) isothermal adsorption result as shown in Figure 3.Respectively according to the corresponding equilibrium adsorption capacity data in figure
And the equilibrium concentration data in solution, with Langmuir model and Freundlich model, result is fitted,
Result shows, two models are relatively good to the matching of adsorption process, wherein the matching effect of Langmuir model
Fruit is more preferable, corresponding coefficient R2Reach 0.9926, the absorption tendency of hint bacterial strain FA1 heavy metal Pb
In monolayer adsorption.It is up to 151.52mg/g according to the maximal absorptive capacity that Langmuir models fitting obtains,
Show that this experiment polycyclic aromatic hydrocarbon-degrading bacteria Herbaspirillum chlorophenolicum FA1 used is to Pb2+'s
Absorbability has competitiveness very much.Have in view of bacterial strain FA1 can effective degrading polycyclic aromatic hydrocarbons simultaneously
Performance, the bacterial strain Herbaspirillum chlorophenolicum FA1 that this experiment is used has good answering
By prospect, the biological restoration of heavy metal-polycyclic aromatic hydrocarbon composite pollution environment is significant.
(4) bacterial strain FA1 is adsorbed Pb by different factors2+Impact
1) bacterial strain FA1 is adsorbed Pb by temperature2+Impact: at Pb2+Concentration is 30mg/L, throwing bacterium amount is 60mg/L,
Under conditions of pH=5.5, respectively 20 DEG C, 25 DEG C, 30 DEG C, carry out batch adsorption experiment at 35 DEG C, absorption
After reaching balance, sampling and measuring Pb2+Concentration, bacterial strain is adsorbed Pb by research temperature2+The impact of effect.
The adsorbent that this adsorption experiment uses is active microorganism, and the change of temperature will directly influence microorganism
Growth and metabolic process, therefore bacterial strain FA1 is adsorbed Pb by temperature2+Efficiency impact notable (Fig. 4).Temperature exists
When 20-30 DEG C, along with the rising of temperature, the clearance of microorganism heavy metal the most substantially increases, along with temperature
Degree is further increased to 35 DEG C, and corresponding clearance reduces on the contrary.Accordingly, polycyclic aromatic hydrocarbon-degrading bacteria FA1 pair is determined
Pb2+Optimal adsorption temperature be 30 DEG C.
2) bacterial strain FA1 is adsorbed Pb by pH2+Impact: at Pb2+Concentration is 30mg/L, throwing bacterium amount is 60mg/L,
Under conditions of temperature 30 DEG C, the pH regulating solution respectively is 2.0,3.0,4.0,5.0,5.5, and absorption reaches balance
After, sampling and measuring Pb2+Concentration, bacterial strain is adsorbed Pb by research pH2+The impact of effect.
Bacterial strain FA1 is adsorbed Pb by pH2+The impact of efficiency is as shown in Figure 5.When relatively low pH value, degradation bacteria FA1
To Pb2+Adsorption rate the lowest, this is because a large amount of hydrogen ions also existed in low ph solution and Pb2+Between
There is the competition on cell surface adsorption site.Along with the change of pH value of solution is big, bearing of microbial cell surface
Electric charge increases, and the beneficially absorption of the lead (II) ion of positively charged reaches the highest when pH is 5.5.In order to more
Illustrate that well pH changes microorganism adsorption Pb2+Impact, arranging of this experiment pH value controls below 6.0,
Because during pH=6.0, precipitation can cause the Pb in solution equally2+Minimizing.Accordingly, it is determined that polycyclic aromatic hydrocarbon
Degradation bacteria FA1 absorption Pb2+Optimum pH be 5.5.
Wherein, in above-mentioned experiment, atomic absorption spectrophotometer flame method is used to measure Pb in sample2+Concentration.
Biological adsorption amount computing formula is:
In formula: q is the adsorbance (mg/g) of antibacterial, c0Being the initial concentration (mg/L) in solution, c is absorption
The concentration (mg/L) of rear solution, v is the volume of solution, and m is biological adsorption agent quality (g) adding solution.
Claims (6)
1. polycyclic aromatic hydrocarbon-degrading bacteria FA1 application in Adsorption of Heavy Metals Pb ion, described polycyclic aromatic hydrocarbon drops
Solve the class named Herbaspirillum chlorophenolicum of bacterium FA1, protect on May 4th, 2010
Being hidden in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preserving number is CGMCC No.
3797。
2. polycyclic aromatic hydrocarbon-degrading bacteria FA1 application in administering heavy metal-polycyclic aromatic hydrocarbon composite pollution, described many
The class named Herbaspirillum chlorophenolicum of PAH degradation bacteria, May 4 in 2010
Day is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, and preserving number is CGMCC
No.3797。
3. polycyclic aromatic hydrocarbon-degrading bacteria FA1 application in the adsorbent for heavy metal preparing resistance to polycyclic aromatic hydrocarbon,
It is characterized in that, described adsorbent is prepared via a method which to obtain: accessed by polycyclic aromatic hydrocarbon-degrading bacteria FA1
Basal medium carries out enrichment culture, then by be in the bacterium solution of exponential phase with 8000rpm freezing from
Heart 10min, the NaCl solution washing with 100～150mM/L 3 times, 4 DEG C of cold preservations are standby.
Application the most according to claim 3, it is characterised in that described basal medium comprises beef
Cream 0.5%, peptone 1.0% and NaCl 0.5%, surplus is deionized water, pH7.0～7.2.
5. according to the application described in any one of claims 1 to 3, it is characterised in that for Adsorption of Heavy Metals
During ion, regulation temperature of reaction system is 20～35 DEG C, and pH is 2～6.
6. according to the application described in any one of claims 1 to 3, it is characterised in that for Adsorption of Heavy Metals
During ion, regulation temperature of reaction system is 25～30 DEG C, and pH is 5～6.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110317760A (en) * | 2019-07-22 | 2019-10-11 | 肇庆学院 | One plant of PAHs- heavy-metal composite pollution degradation/adhered bacteria and its application in environmental pollution reparation |
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| CN110317760B (en) * | 2019-07-22 | 2020-09-29 | 肇庆学院 | PAHs-heavy metal combined pollution degrading/adsorbing bacterium and application thereof in environmental pollution remediation |
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