CN105861366A - Application of polycyclic aromatic hydrocarbon degrading strain FA1 in absorption of heavy metal Pb ions - Google Patents

Application of polycyclic aromatic hydrocarbon degrading strain FA1 in absorption of heavy metal Pb ions Download PDF

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CN105861366A
CN105861366A CN201610245173.9A CN201610245173A CN105861366A CN 105861366 A CN105861366 A CN 105861366A CN 201610245173 A CN201610245173 A CN 201610245173A CN 105861366 A CN105861366 A CN 105861366A
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徐红霞
李宵慧
吴吉春
施小清
孙媛媛
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Nanjing University
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    • C12R2001/01Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B09DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
    • B09CRECLAMATION OF CONTAMINATED SOIL
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    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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    • C02F3/34Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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    • C02F2101/20Heavy metals or heavy metal compounds
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00Nature of the contaminant
    • C02F2101/30Organic compounds
    • C02F2101/32Hydrocarbons, e.g. oil
    • C02F2101/327Polyaromatic Hydrocarbons [PAH's]

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Abstract

The invention discloses application of a polycyclic aromatic hydrocarbon degrading strain FA1 in absorption of heavy metal Pb ions. The category of the polycyclic aromatic hydrocarbon degrading strain FA1 is named as herbaspirillum chlorophenolicum; the polycyclic aromatic hydrocarbon degrading strain FA1 is preserved in China General Microbiological Culture Collection Center (CGMCC) in May 4th, 2010, the preservation address is No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No. 3797. The invention provides the strain and gene material for repairing microorganisms which have heavy metal and polycyclic aromatic hydrocarbon combined pollution to the environment.

Description

Polycyclic aromatic hydrocarbon-degrading bacteria FA1 application in Adsorption of Heavy Metals Pb ion
Technical field
Present invention relates particularly to polycyclic aromatic hydrocarbon-degrading bacteria FA1 application in Adsorption of Heavy Metals Pb ion.
Background technology
Due to the factors such as mining, waste gas discharge, solid waste accumulation and sewage irrigation, a large amount of poisonous and harmful weights Metal enters in environment and continues to build up.Heavy metal contaminants is degraded the most hardly, and along with food Thing chain and be enriched with, there is the strongest bioconcentration so that ecological environment and human health face serious threat. As one of heavy metal that toxicity is maximum, lead concentration over-standard phenomenon in China's soil and groundwater environment is frequent Be seen in report.In recent years, heavy metal pollution with lead as representative causes environment and social problem are increasingly Prominent, therefore, the reparation of Heavy Metals In Environment is the most singly one of the study hotspot in current environment protection field, Have more important realistic meaning.
Restorative procedure currently for heavy metal mainly has peripheral doses, chemical redemption and biological restoration etc..Biological The features such as restorative procedure has efficiently, low consumption, economy, environmental friendliness and easily operated management, than traditional Physics, chemical restoration have clear superiority, in particular for the heavy metal pollution situation of low concentration.This skill It is critical only that of art filters out the microorganism with stronger Adsorption of Heavy Metals ability.Can micro-life of Adsorption of Heavy Metals Thing includes antibacterial, fungus and algae etc., and wherein, antibacterial is the most excellent due to its strongest biochemical adaptability Gesture.
It is worthy of note, the heavy metal biological of existing report is repaired mostly for single heavy metal pollution feelings Shape, but the heavy metal pollution in environment often occurs with other organic pollutions simultaneously.As heavy metal-organic The Typical Representative of thing combined pollution, heavy metal and polycyclic aromatic hydrocarbon composite pollution phenomenon are in China's soil and groundwater It is widely present.When two kinds of pollutant coexist, the toxic effect of polycyclic aromatic hydrocarbon is by can the microorganism of Adsorption of Heavy Metals Cause and coerce, harm the adsorption to its heavy metal.Therefore, for the heavy metal in actual environment-many PAH combined pollution present situation, it is necessary to filtering out resistance to polycyclic aromatic hydrocarbon toxicity even can the weight of degrading polycyclic aromatic hydrocarbons Metal adsorption microorganism, studies its characterization of adsorption, for heavy metal and the microorganism of polycyclic aromatic hydrocarbon composite pollution environment Repair and bacterial strain and genetic material are provided.
Summary of the invention
By investigating the adsorptivity of the polycyclic aromatic hydrocarbon-degrading bacteria heavy metal Pb ion of the existing different genera of seminar Can, present invention discover that studying polycyclic aromatic hydrocarbon-degrading bacteria FA1 heavy metal Pb filtered out before has good Toleration and adsorptivity.The named Herbaspirillum of class of this polycyclic aromatic hydrocarbon-degrading bacteria FA1 Chlorophenolicum, is preserved in China Committee for Culture Collection of Microorganisms on May 4th, 2010 Common micro-organisms center, preservation address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, and preserving number is CGMCC No.3797.Screening technique and the bacterial strain performance of this polycyclic aromatic hydrocarbon-degrading bacteria FA1 are shown in seminar XU Deng article " the Degradation of fluoranthene by a newly isolated strain of delivered for 2011 Herbaspirillum chlorophenolicum from activated sludge ", Biodegradation, 22 (2), 335-345。
In view of it is for the adsorption of heavy metal Pb ion, present invention further proposes above-mentioned polycyclic aromatic hydrocarbon The application in administering heavy metal-polycyclic aromatic hydrocarbon composite pollution of degradation bacteria FA1.
Further, the invention allows for above-mentioned polycyclic aromatic hydrocarbon-degrading bacteria FA1 and prepare resistance to polycyclic aromatic hydrocarbon Adsorbent for heavy metal in application, described adsorbent is prepared via a method which to obtain: by polycyclic aromatic hydrocarbon Degradation bacteria FA1 accesses in basal medium and carries out enrichment culture, then by be in the bacterium solution of exponential phase with 8000rpm frozen centrifugation 10min, the NaCl solution washing with 100~150mM/L 3 times, 4 DEG C of cold preservations are standby With.
Preferably, described basal medium comprises Carnis Bovis seu Bubali cream 0.5%, peptone 1.0% and NaCl 0.5%, Surplus is deionized water, pH7.0~7.2.
In above-mentioned application, when for Adsorption of Heavy Metal Ions, for ensureing adsorption effect, reactant should be regulated Be temperature be 20~35 DEG C, pH is 2~6.
It is highly preferred that when for Adsorption of Heavy Metal Ions, regulation temperature of reaction system is 25~30 DEG C, pH It is 5~6.Most preferably, when for Adsorption of Heavy Metal Ions, regulation temperature of reaction system is 30 DEG C, pH It is 5.5.
Beneficial effect: the present invention have unexpectedly discovered that can simultaneously degrading polycyclic aromatic hydrocarbons and heavy metal ion multi-ring Biodegradation of PAHs bacterium Herbaspirillum chlorophenolicum FA1, and by studying its characterization of adsorption, attach most importance to The microorganism remediation of metal and polycyclic aromatic hydrocarbon composite pollution environment provides bacterial strain and genetic material.
Accompanying drawing explanation
Fig. 1 is that bacterial strain FA1 is at variable concentrations Pb2+Growth curve in solution;
Fig. 2 is that bacterial strain FA1 is to Pb2+Adsorption dynamics adsorption kinetics;
Fig. 3 is that bacterial strain FA1 is to Pb2+Adsorption isotherm;
Fig. 4 is that bacterial strain FA1 is adsorbed Pb by temperature2+The impact of efficiency;
Fig. 5 is that bacterial strain FA1 is adsorbed Pb by pH2+The impact of efficiency.
Detailed description of the invention
The present invention be may be better understood according to following embodiment.But, those skilled in the art is easy to understand , the content described by embodiment is merely to illustrate the present invention, and should be also without limitation on claims The present invention described in detail by.
The preparation of embodiment 1 heavy metal absorbent.
The present embodiment microorganism used therefor is from the polycyclic aromatic hydrocarbon-degrading bacteria filtered out before seminar Herbaspirillum chlorophenolicum FA1 (Xu et al.2011, Degradation of fluoranthene by a newly isolated strain of Herbaspirillum chlorophenolicum from activated Sludge ", Biodegradation, 22 (2), 335-345).Bacterial strain FA1 is in the GenBank data of NCBI Sequence accession number in storehouse is HM107777, is preserved in Chinese microorganism strain on May 4th, 2010 Preservation administration committee common micro-organisms center, preservation address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Number, Institute of Microorganism, Academia Sinica, register on the books numbered CGMCC No.3797.
Sorbent preparation method: before inoculation, accesses bacterial strain FA1 in basal medium and carries out enrichment culture, so After by being in the bacterium solution of exponential phase with 8000rpm frozen centrifugation 10min, with the NaCl solution of 150mM/L Washing 3 times, obtain adsorbent, 4 DEG C of cold preservations are standby.
Embodiment 2 microorganism heavy metal Pb2+Characterization of adsorption research.
All experimental grouies of following characterization of adsorption research are equipped with 3 parallel and blank groups.
(1) microorganism heavy metal Pb2+Toleration
Take Pb (NO3)2, add basal medium so that Pb2+Concentration is respectively 0,10,20,50,100, 200mg/L, and to regulate pH value of solution be 5.5, inoculates into bacterial strain FA1, in concussion incubator 30 DEG C, 125rpm Constant temperature culture, timing sampling, the OD of solution is measured with ultraviolet spectrophotometer600Value, draws with the time as horizontal stroke The growth curve of coordinate.
Polycyclic aromatic hydrocarbon-degrading bacteria FA1 is at variable concentrations Pb2+Growth curve under existence condition is as shown in Figure 1. With without Pb2+Comparison compare, as Pb in culture medium2+When concentration is 10mg/L, the growth of bacterial strain FA1 It is barely affected.Along with Pb2+The further increase of concentration, the speed of growth of bacterial strain gradually slows down, Biomass Corresponding OD600Value diminishes the most therewith, and Pb is described2+The growth and breeding of bacterial strain there is certain inhibitory action.But It is to it is worthy of note, the Pb of higher concentration2+Concentration (20-200mg/L) does not delay bacterial strain to enter Exponential phase.At Pb2+During at concentrations up to 200mg/L, it is the Biomass of (15h) in exponential phase Compared to without Pb2+For the matched group of concentration, only only reduce 28.3%, show Herbaspirillum Chlorophenolicum FA1 heavy metal lead has good toleration, has the value of research further.
(2) adsorption dynamics adsorption kinetics
Adsorbent prepared by embodiment 1 is accessed to the Pb (NO containing 30mg/L3)2The politef of solution In centrifuge tube, the access concentration of adsorbent is 60mg/L, sets in background solution the concentration of NaCl as 150 MM/L, the initial pH of regulation system are 5.5.Adsorption reaction system is placed in isothermal vibration incubator, arranges Rotating speed is 125rpm, and temperature is 30 DEG C, samples at regular intervals, filter membrane (0.22 μm), uses atom Absorption spectrophotometer (AAS) measures Pb remaining in solution2+Concentration.
Polycyclic aromatic hydrocarbon-degrading bacteria FA1 Adsorption of Heavy Metals lead is had a major impact by the time of contact of cell and lead ion. Using its adsorption dynamics adsorption kinetics of batch Research on experimental methods, result is as shown in Figure 2.At the absorption initial stage, bacterial strain is to molten In liquid, the absorption of lead ion is more rapid, and 5h adsorbance i.e. reaches during adsorption equilibrium total adsorbance more than half, Adsorbance increase afterwards slows down, and the rate of adsorption declines.After 25h, adsorbance reaches total adsorbance during adsorption equilibrium 80%, bacterial strain is to Pb2+Absorption after 80h, reach complete equipilibrium.
To Pb in bacterial strain FA1 adsorbent solution2+Dynamics data be analyzed matching, find pseudo-second order kinetic With Elovich kinetic model, the fitting effect of experimental result is compared other models preferably, the phase relation of matching Number R2The highest, respectively 0.986 and 0.9643.Meet pseudo-second order kinetic relation table detail bacterium FA1 pair Pb in solution2+Absorption be exchanged for main with chemisorbed or ion, and Elovich model is to experimental result matching Good then explanation Herbaspirillum chlorophenolicum FA1 to Pb2+Biological adsorption be one non-all The diffusion process of phase.
(3) adsorption isotherm experiment
Initial p b of series is set2+Concentration is 5,10,20,30,50,100,200mg/L, adsorbs power in 2 The experiment condition learned carries out the adsorption isotherm experiment at 30 DEG C, and after absorption reaches balance, the balance of sampling and measuring lead is dense Degree and adsorbance.
At 30 DEG C, bacterial strain FA1 (dosage 60mg/L) Pb in solution (pH=5.5)2+(the denseest Degree 30mg/L) isothermal adsorption result as shown in Figure 3.Respectively according to the corresponding equilibrium adsorption capacity data in figure And the equilibrium concentration data in solution, with Langmuir model and Freundlich model, result is fitted, Result shows, two models are relatively good to the matching of adsorption process, wherein the matching effect of Langmuir model Fruit is more preferable, corresponding coefficient R2Reach 0.9926, the absorption tendency of hint bacterial strain FA1 heavy metal Pb In monolayer adsorption.It is up to 151.52mg/g according to the maximal absorptive capacity that Langmuir models fitting obtains, Show that this experiment polycyclic aromatic hydrocarbon-degrading bacteria Herbaspirillum chlorophenolicum FA1 used is to Pb2+'s Absorbability has competitiveness very much.Have in view of bacterial strain FA1 can effective degrading polycyclic aromatic hydrocarbons simultaneously Performance, the bacterial strain Herbaspirillum chlorophenolicum FA1 that this experiment is used has good answering By prospect, the biological restoration of heavy metal-polycyclic aromatic hydrocarbon composite pollution environment is significant.
(4) bacterial strain FA1 is adsorbed Pb by different factors2+Impact
1) bacterial strain FA1 is adsorbed Pb by temperature2+Impact: at Pb2+Concentration is 30mg/L, throwing bacterium amount is 60mg/L, Under conditions of pH=5.5, respectively 20 DEG C, 25 DEG C, 30 DEG C, carry out batch adsorption experiment at 35 DEG C, absorption After reaching balance, sampling and measuring Pb2+Concentration, bacterial strain is adsorbed Pb by research temperature2+The impact of effect.
The adsorbent that this adsorption experiment uses is active microorganism, and the change of temperature will directly influence microorganism Growth and metabolic process, therefore bacterial strain FA1 is adsorbed Pb by temperature2+Efficiency impact notable (Fig. 4).Temperature exists When 20-30 DEG C, along with the rising of temperature, the clearance of microorganism heavy metal the most substantially increases, along with temperature Degree is further increased to 35 DEG C, and corresponding clearance reduces on the contrary.Accordingly, polycyclic aromatic hydrocarbon-degrading bacteria FA1 pair is determined Pb2+Optimal adsorption temperature be 30 DEG C.
2) bacterial strain FA1 is adsorbed Pb by pH2+Impact: at Pb2+Concentration is 30mg/L, throwing bacterium amount is 60mg/L, Under conditions of temperature 30 DEG C, the pH regulating solution respectively is 2.0,3.0,4.0,5.0,5.5, and absorption reaches balance After, sampling and measuring Pb2+Concentration, bacterial strain is adsorbed Pb by research pH2+The impact of effect.
Bacterial strain FA1 is adsorbed Pb by pH2+The impact of efficiency is as shown in Figure 5.When relatively low pH value, degradation bacteria FA1 To Pb2+Adsorption rate the lowest, this is because a large amount of hydrogen ions also existed in low ph solution and Pb2+Between There is the competition on cell surface adsorption site.Along with the change of pH value of solution is big, bearing of microbial cell surface Electric charge increases, and the beneficially absorption of the lead (II) ion of positively charged reaches the highest when pH is 5.5.In order to more Illustrate that well pH changes microorganism adsorption Pb2+Impact, arranging of this experiment pH value controls below 6.0, Because during pH=6.0, precipitation can cause the Pb in solution equally2+Minimizing.Accordingly, it is determined that polycyclic aromatic hydrocarbon Degradation bacteria FA1 absorption Pb2+Optimum pH be 5.5.
Wherein, in above-mentioned experiment, atomic absorption spectrophotometer flame method is used to measure Pb in sample2+Concentration.
Biological adsorption amount computing formula is:
In formula: q is the adsorbance (mg/g) of antibacterial, c0Being the initial concentration (mg/L) in solution, c is absorption The concentration (mg/L) of rear solution, v is the volume of solution, and m is biological adsorption agent quality (g) adding solution.

Claims (6)

1. polycyclic aromatic hydrocarbon-degrading bacteria FA1 application in Adsorption of Heavy Metals Pb ion, described polycyclic aromatic hydrocarbon drops Solve the class named Herbaspirillum chlorophenolicum of bacterium FA1, protect on May 4th, 2010 Being hidden in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preserving number is CGMCC No. 3797。
2. polycyclic aromatic hydrocarbon-degrading bacteria FA1 application in administering heavy metal-polycyclic aromatic hydrocarbon composite pollution, described many The class named Herbaspirillum chlorophenolicum of PAH degradation bacteria, May 4 in 2010 Day is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, and preserving number is CGMCC No.3797。
3. polycyclic aromatic hydrocarbon-degrading bacteria FA1 application in the adsorbent for heavy metal preparing resistance to polycyclic aromatic hydrocarbon, It is characterized in that, described adsorbent is prepared via a method which to obtain: accessed by polycyclic aromatic hydrocarbon-degrading bacteria FA1 Basal medium carries out enrichment culture, then by be in the bacterium solution of exponential phase with 8000rpm freezing from Heart 10min, the NaCl solution washing with 100~150mM/L 3 times, 4 DEG C of cold preservations are standby.
Application the most according to claim 3, it is characterised in that described basal medium comprises beef Cream 0.5%, peptone 1.0% and NaCl 0.5%, surplus is deionized water, pH7.0~7.2.
5. according to the application described in any one of claims 1 to 3, it is characterised in that for Adsorption of Heavy Metals During ion, regulation temperature of reaction system is 20~35 DEG C, and pH is 2~6.
6. according to the application described in any one of claims 1 to 3, it is characterised in that for Adsorption of Heavy Metals During ion, regulation temperature of reaction system is 25~30 DEG C, and pH is 5~6.
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110317760A (en) * 2019-07-22 2019-10-11 肇庆学院 One plant of PAHs- heavy-metal composite pollution degradation/adhered bacteria and its application in environmental pollution reparation
CN111438180A (en) * 2019-01-16 2020-07-24 四川千路环保科技有限责任公司 Soil remediation granules and preparation method thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102943052A (en) * 2012-11-22 2013-02-27 台州职业技术学院 Heavy metal-resistant polycyclic aromatic hydrocarbon (PAHs) degrading bacteria and application thereof in remediation of composite contaminated soil
CN103755039A (en) * 2013-12-30 2014-04-30 兰州石化职业技术学院 Application of compound microorganism bacterium agent in treatment of petrochemical sewage and sludge
CN104475444A (en) * 2014-11-04 2015-04-01 上海交通大学 Preparation, application and usage method for biocarbon-immobilized composite pollution-degrading bacterium particle

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102943052A (en) * 2012-11-22 2013-02-27 台州职业技术学院 Heavy metal-resistant polycyclic aromatic hydrocarbon (PAHs) degrading bacteria and application thereof in remediation of composite contaminated soil
CN103755039A (en) * 2013-12-30 2014-04-30 兰州石化职业技术学院 Application of compound microorganism bacterium agent in treatment of petrochemical sewage and sludge
CN104475444A (en) * 2014-11-04 2015-04-01 上海交通大学 Preparation, application and usage method for biocarbon-immobilized composite pollution-degrading bacterium particle

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
XU HONGXIA ET AL.: "Degradation of fluoranthene by a newly isolated strain of Herbaspirillum chlorophenolicum from activated sludge", 《BIODEGRADATION》 *
孙业政: "白腐菌Phanerochaete chrysosporium对多环芳烃与重金属复合污染的处理", 《中国优秀硕士学位论文全文数据库.工程科技I辑》 *
曾光明 等: "Cd(Ⅱ)对多环芳烃降解菌Bacillus sp.P1产酶及酶促降解过程影响研究", 《湖南大学学报(自然科学版)》 *
李丽 等: "枯草芽孢杆菌对多环芳烃的降解能力研究", 《河南农业科学》 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111438180A (en) * 2019-01-16 2020-07-24 四川千路环保科技有限责任公司 Soil remediation granules and preparation method thereof
CN110317760A (en) * 2019-07-22 2019-10-11 肇庆学院 One plant of PAHs- heavy-metal composite pollution degradation/adhered bacteria and its application in environmental pollution reparation
CN110317760B (en) * 2019-07-22 2020-09-29 肇庆学院 PAHs-heavy metal combined pollution degrading/adsorbing bacterium and application thereof in environmental pollution remediation

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