CN103755039A - Application of compound microorganism bacterium agent in treatment of petrochemical sewage and sludge - Google Patents

Application of compound microorganism bacterium agent in treatment of petrochemical sewage and sludge Download PDF

Info

Publication number
CN103755039A
CN103755039A CN201310742337.5A CN201310742337A CN103755039A CN 103755039 A CN103755039 A CN 103755039A CN 201310742337 A CN201310742337 A CN 201310742337A CN 103755039 A CN103755039 A CN 103755039A
Authority
CN
China
Prior art keywords
microbial inoculum
complex microbial
sludge
sewage
bio
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
CN201310742337.5A
Other languages
Chinese (zh)
Inventor
张满效
谢小冬
张威
陈拓
刘光琇
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Lanzhou Petrochemical College of Vocational Technology
Original Assignee
Lanzhou Petrochemical College of Vocational Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lanzhou Petrochemical College of Vocational Technology filed Critical Lanzhou Petrochemical College of Vocational Technology
Priority to CN201310742337.5A priority Critical patent/CN103755039A/en
Publication of CN103755039A publication Critical patent/CN103755039A/en
Priority to CN201410578342.1A priority patent/CN104310594B/en
Withdrawn legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/10—Inorganic compounds
    • C02F2101/20—Heavy metals or heavy metal compounds
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/30—Organic compounds
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
    • C02F2103/34—Nature of the water, waste water, sewage or sludge to be treated from industrial activities not provided for in groups C02F2103/12 - C02F2103/32
    • C02F2103/36—Nature of the water, waste water, sewage or sludge to be treated from industrial activities not provided for in groups C02F2103/12 - C02F2103/32 from the manufacture of organic compounds
    • C02F2103/365—Nature of the water, waste water, sewage or sludge to be treated from industrial activities not provided for in groups C02F2103/12 - C02F2103/32 from the manufacture of organic compounds from petrochemical industry (e.g. refineries)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Microbiology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Hydrology & Water Resources (AREA)
  • Engineering & Computer Science (AREA)
  • Environmental & Geological Engineering (AREA)
  • Water Supply & Treatment (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention provides an application of a compound microorganism bacterium agent in treatment of petrochemical sewage and sludge. The application comprises the following steps: (1) preparing a biological carrier, to be specific, adding glutaraldehyde to polyphenylene oxide, suspending, stirring, washing, then adding a PBS (Phosphate Buffer Solution) containing CaCl2 and standing; (2) carrying out enlarging cultivation of the compound microorganism bacterium agent; (3) putting the compound microorganism bacterium agent subjected to enlarging cultivation, obtained in the step (2), in the biological carrier prepared in the step (1); and (4) adding the biological carrier loaded with the compound microorganism bacterium agent, obtained in the step (3), to the petrochemical sewage sludge, wherein the compound microorganism bacterium agent continuously increases to a stable period and forms a biological membrane on the surface of the biological carrier, and the biological membrane metabolizes, adsorbs, absorbs, digests and resolves organic pollutants and heavy metals in petrochemical sewage and sludge, so that the petrochemical sewage and sludge are converted into stable harmless materials. Through research, the applicant finds that the compound microorganism bacterium agent can be used for treating the petrochemical sewage and sludge very well.

Description

A kind of complex microbial inoculum is in the application of processing in petrifaction sewage mud
Technical field
The invention belongs to microbial technology field, be specifically related to a kind of complex microbial inoculum in the application of processing in petrifaction sewage mud.
Background technology
Utilize at present the research of microbiological treatment trade effluent PetroChina Company Limited. chemical engineering sewage mud also few, can be used for effective oil degradation bacteria of water treatment still less.Lu Xianyan etc. isolate 2 strain bacterial strains from Guangzhou petrifaction sewage treatment plant waste water, use the oily(waste)water of autogamy to do experimental water, and through the processing of 7 days, 2 strain bacterial strain COD clearances were respectively 70% and 50%; The highest COD clearance is 83.67% and 57.49%.
Summary of the invention
The invention provides a kind of complex microbial inoculum in the application of processing in petrifaction sewage mud, can well process petrifaction sewage mud.
The invention provides a kind of complex microbial inoculum in the application of processing in petrifaction sewage mud.
Preferably, the step of described application is as follows:
1) prepare bio-carrier: in polyphenylene oxide, add glutaraldehyde to suspend and stir, after washing, add containing CaCl 2pBS damping fluid standing;
2) enlarged culturing of complex microbial inoculum;
3) complex microbial inoculum after enlarged culturing step (2) being obtained is put into bio-carrier prepared by step (1);
4) bio-carrier that is loaded with complex microbial inoculum step (3) being obtained adds in petrifaction sewage, complex microbial inoculum continued to rise in value to stationary phase, on bio-carrier surface, form microbial film, carry out metabolism, organic pollutant and heavy metal in absorption, absorption, digestion, decomposition petrifaction sewage and mud, make it to transform into stable innoxious material.
Preferably, enlarged culturing described in step (2) is that complex microbial inoculum is coated in LB solid medium, shaking culture 24-48 hour on 20-30 ℃, the shaking table of 150 rpm/min, to complex microbial inoculum concentration be 10 3individual/ml.
Further, the temperature of described shaking culture is 30 ℃.
The composite fungus agent that shaking culture obtains in this temperature range, the surviving rate of composite fungus agent is high, active strong.
Preferably, the complex microbial inoculum after the enlarged culturing in step (3), step (2) being obtained is put into after bio-carrier prepared by step (1), at 20-25 ℃, stirs 30min, and 4 ℃ standing 48 hours.
Further, the temperature of described stirring is 20 ℃.
At this temperature, stir, complex microbial inoculum well can be fixed on bio-carrier.
The applicant finds by research, and the complex microbial inoculum of being presented by Chinese Academy of Sciences cold area arid region environment and Engineering research institute can well be processed the sewage sludge of petrochemical industry.
The applicant further analyzes it, finds that this microbiobacterial agent is comprised of 22 kinds of bacterial strains.Every kind of bacterial strain all has good treatment effect to CODcr.
The applicant is further studied the three strain bacterial strains of Rhodopseudomonas wherein, finds that Rhodopseudomonas lsd03, Rhodopseudomonas lsd05 bacterial strain Pyrogentisinic Acid have good treatment effect; Rhodopseudomonas BDP01 bacterial strain has treatment effect very to naphthalene, anthracene.
Complex microbial inoculum of the present invention reaches as high as 87.1% to pollutant removal CODcr; By to threatening maximum arene compounds Rhodopseudomonas 3 identification of strains and research in the environment of degrading in flora, find: lsd03, the lsd05 of Rhodopseudomonas are at 0-1000 mgL -1phenol concentration in well-grown, high density is issued to will the growing of time low concentration of logarithmic phase, the phenol that the Extending culture time can degrading high concentration; The BDP01 of Rhodopseudomonas has good degrading polycyclic aromatic hydrocarbons ability, can accumulate the naphthalene of degraded 89.64% and 77.21% anthracene in 15d, adds in right amount the ability of Tween80 degraded naphthalene and brings up to 96.132%, and the ability of degraded anthracene brings up to 89.916%.
Accompanying drawing explanation
Fig. 1 numbers the clearance of 1-7 bacterial strain for CODcr in complex microbial inoculum;
Fig. 2 numbers the clearance of 8-14 bacterial strain for CODcr in complex microbial inoculum;
Fig. 3 numbers the clearance of 15-22 bacterial strain for CODcr in complex microbial inoculum;
Fig. 4 a is the OD of lsd03 in different concns phenol 600value, 4b is the OD of lsd05 in different concns phenol 600value;
Fig. 5 is the impact of inoculum size on BDP01 degraded naphthalene;
Fig. 6 is the impact of inoculum size on BDP01 degraded anthracene.
Embodiment
Following embodiment is convenient to understand better the present invention, but does not limit the present invention.Experimental technique in following embodiment, if no special instructions, is ordinary method.Test materials used in following embodiment, if no special instructions, is and purchases available from routine biochemistry reagent shop.Quantitative test in following examples, all arranges and repeats experiment, results averaged for three times.
The complex microbial inoculum using in the present invention is presented by Chinese Academy of Sciences cold area arid region environment and Engineering research institute's environmental microorganism laboratory.
embodiment 1
The method that using microbe composite fungus agent is processed petrifaction sewage mud is as follows:
1, the preparation of bio-carrier: be 250 μ m in aperture, surface-area is 60m 2in the 1g polyphenylene oxide of/g, add 50ml 5% glutaraldehyde to suspend and stir 48 hours, with 2, the washing of 4-dinitrobenzene hydrazine, wash, add the CaCl containing 0.75mol/L 250mmol/L PBS(pH5.7) buffer chamber is gentle and quiet puts 12 hours; Make microorganism be easy to conjunctiva procreation existence, microorganism is easy to fix;
2, complex microbial inoculum is coated in LB solid medium, shaking culture 24-48 hour on 30 ℃, the shaking table of 150rpm/min, to complex microbial inoculum concentration be 10 3individual/ml;
3, the 50ml complex microbial inoculum suspension of being prepared by step (2) is put into 6g bio-carrier prepared by step (1), at 20 ℃, stirs 30min, 4 ℃ standing 48 hours, on bio-carrier, form gradually flora microbial film, be filtered dry stand-by.
4, first petrifaction sewage is passed through to grid, grid gap 6mm, removes Gate slag, and to tackle suspended contamination thick in sewage, removal may be stopped up the thicker suspended substance of water pump assembly and pipeline valve, and guarantees that subsequent disposal facility can normally move; By oil trap, oil trap is mainly for separating of the oil product of removing suspended state in waste water again, and its specification is determined according to discharge size.When petrifaction sewage passes through oil trap, floatation surface on oil product, by oil header, be transported to and in oil header, flow into water knockout, the heavy oil precipitating and other impurity, accumulate in pool bottom sludge bucket, by shore pipe, enter in sludge pipe, waste water through oil removal processing enters in equalizing tank, at 20-35 ℃, the bio-carrier that is loaded with complex microbial inoculum that step (3) is obtained drops in equalizing tank, complex microbial inoculum continued to rise in value to stationary phase, on bio-carrier surface, form microbial film, carry out metabolism, absorption, absorb, digestion, decompose organic pollutant and heavy metal in sewage and mud, make it to transform into stable innoxious material.
embodiment 2
The method that using microbe composite fungus agent is processed petrifaction sewage mud is as follows:
1, the preparation of bio-carrier: be 200 μ m in aperture, surface-area is 70m 2in the 1g polyphenylene oxide of/g, add 50ml 5% glutaraldehyde to suspend and stir 48 hours, with 2, the washing of 4-dinitrobenzene hydrazine, wash, add the CaCl containing 0.75mol/L 250mmol/L PBS(pH5.7) buffer chamber is gentle and quiet puts 12 hours; Make microorganism be easy to conjunctiva procreation existence, microorganism is easy to fix;
2, complex microbial inoculum is coated in LB solid medium, shaking culture 24-48 hour on 20 ℃, the shaking table of 150rpm/min, to complex microbial inoculum concentration be 10 3individual/ml;
3, the 50ml complex microbial inoculum suspension of being prepared by step (2) is put into 6g bio-carrier prepared by step (1), at 25 ℃, stirs 30min, 4 ℃ standing 48 hours, on bio-carrier, form gradually flora microbial film, be filtered dry stand-by.
4, first petrifaction sewage is passed through to grid, grid gap 6mm, removes Gate slag, and to tackle suspended contamination thick in sewage, removal may be stopped up the thicker suspended substance of water pump assembly and pipeline valve, and guarantees that subsequent disposal facility can normally move; By oil trap, oil trap is mainly for separating of the oil product of removing suspended state in waste water again, and its specification is determined according to discharge size.When petrifaction sewage passes through oil trap, floatation surface on oil product, by oil header, be transported to and in oil header, flow into water knockout, the heavy oil precipitating and other impurity, accumulate in pool bottom sludge bucket, by shore pipe, enter in sludge pipe, waste water through oil removal processing enters in equalizing tank, at 20-35 ℃, the bio-carrier that is loaded with complex microbial inoculum that step (3) is obtained drops in equalizing tank, complex microbial inoculum continued to rise in value to stationary phase, on bio-carrier surface, form microbial film, carry out metabolism, absorption, absorb, digestion, decompose organic pollutant and heavy metal in sewage and mud, make it to transform into stable innoxious material.
The method that is below the complex microbial inoculum processing petrifaction sewage mud described in the application embodiment of the present invention 1 is carried out the concrete result data of testing.
Test sewage is taken from Lanzhou Petrochemical sewage treatment plant inflow mouth, applies the method for complex microbial inoculum processing petrifaction sewage mud of the present invention and process front and back indices referring to table 1 in sewage.
Table 1
Figure 2013107423375100002DEST_PATH_IMAGE001
。
After measured, complex microbial inoculum of the present invention is comprised of 22 kinds of bacterium, under 22 kinds of bacterium, belong to and in complex microbial inoculum shared mass fraction in Table 2.
Table 2 complex microbial inoculum and the concrete mass fraction of each component
Figure 724026DEST_PATH_IMAGE001
。
Table 3 and Fig. 1-3rd, in table 2, each single bacterial strain is applied respectively the chemical oxygen demand (COD) that above-mentioned processing petrifaction sewage mud method records with potassium dichromate process for CODcr() removal effect.
The clearance (%) of table 3 different strain to CODcr
Figure 2013107423375100002DEST_PATH_IMAGE003
。
From table 3 and Fig. 1-3, can find out, at first 3 days, bacterial classification 3,11,12,14, s1, s2, s3, s4, s5, w1, w5 and w7 all have good treatment effect, and CODcr clearance all reaches 50%.The removal effect of bacterial classification 1 is not fine, and clearance does not also reach 10%.And bacterial classification 2,4,13, and s3 also has good effect, clearance has all reached more than 45%.The CODcr clearance of other bacterial classification is all between 20%-40%.Through processing in 6 days, the CODcr clearance of most of bacterium had further raising.By the 9th day, except bacterial classification 1,6,7,8 and 10, the clearance of all bacteriums all reaches more than 50%.Final most of bacterium can reach more than 70% removal effect, has obtained desirable effect.
Through comparison, most of bacterium ( bacterial classification 3,11,12,14, s1, s2, s3, s4, s5, w1, w5 and w7) in early stage, just obtained good treatment effect, along with continuing degraded, finally all obtain good degradation rate, the environment of the adaptation experiment sewage that these bacteriums can be very fast is described.Along with the continuation of degraded, degradation rate declines to some extent, and this may be the carrying out along with degraded, and in experiment sewage, organic concentration reduces gradually much relations.Along with the reduction of nutritive substance in sewage, the easily composition minimizing of degraded of experimental strain in water sample, remaining is that it is compared with the composition of difficult degradation.Originally a large amount of microorganisms in sewage are because the rareness of nutritive substance and death or activity decreased, thereby cause decline or the death of the degradation rate of bacterium.Also may be the growth and breeding that the accumulation of some meta-bolites of experimental strain in water sample can suppress himself cell. Bacterial classification 1,5,6,9 and 10 is poor at the treatment effect in early stage, with efficiency of post treatment, increases, and finally also can reach more than 70% CODcr clearance.Perhaps, this is that these bacterial classifications need to be through the adaptation of longer for some time to sewage.Through adaptation after a while, bacterial classification has adapted to the environment of experiment sewage, and processing efficiency has also just had larger lifting naturally.Wherein bacterial classification 1 was through the continuous processing of 12 days, and processing rate does not also reach 50%; Bacterial classification 6 and 10 did not reach 50% through 9 days yet.Bacterial classification 7 is with 8 compared with other bacterial classifications, and treatment effect is not fine, and through the processing of 18 days, the CODcr of sewage was still 683.74mg/L and 558.27mg/L, and clearance is respectively 54.4% and 62.8%.Except bacterial classification 7,8 and w5, other bacterial classification was through the continuous processing of 18 days, and the processing CODcr of sewage all can reach more than 73.3%.It is good to the treatment effect of sewage that above-mentioned experiment further illustrates complex microbial inoculum of the present invention.
The applicant has further studied Rhodopseudomonas lsd03 in complex microbial inoculum, the impact on increment on different phenol concentration of Rhodopseudomonas lsd05 bacterial strain.
Phenol liquid selective medium preparation method: by K 2hPO 45.17g, KH 2pO 41.7g adds ddH 2o is mixed with 10mL salts solution, then adds ddH 2o to 1000mL, adjusts pH 7.0-7.2, and 121 ℃ of sterilizing 20min, quantitatively add the phenol (121 ℃ of sterilizings) after degerming after cooling again.
By lsd03, lsd05 bacterial strain not containing in 50 mL LB liquid nutrient mediums of phenol 30 ℃, 150 rmin -1concussion is cultivated 24h to logarithmic phase respectively, by 1 ﹪ inoculum size, be inoculated into respectively in the 50 mL phenol liquid selective medium that contain different concns phenol (0,400,700,1000,1300,1500,1700,2000 mgL-1), and 30 ℃, 150 rmin -1concussion is cultivated, and every 12h gets sample one time, measures its OD 600, to 120h.
Result shows, lsd03, lsd05 bacterial strain are at 1300-2000 mgL -1in the phenol of concentration, growth all approaches stagnant condition, but at 0-1000 mgL -1phenol concentration in well-grown (referring to fig. 4lsd03 a,lsd05 b).This result shows that the maximum phenol tolerance concentration of two bacterial strains is 1000 mgL -1.But at 1000 mgL -1under phenol concentration, bacterial strain reaches will the growing of time low concentration of logarithmic phase, therefore in practice, makes the phenol of its degrading high concentration, can proper extension incubation time.
The applicant has also studied the degradation effect of Rhodopseudomonas BDP01 bacterial strain to naphthalene, anthracene in complex microbial inoculum.
BDP01 bacterial strain is logarithmic growth in 48h in LB liquid nutrient medium, and 48h enters to 72h the stationary phase of growing, and starts to enter decline phase after 72 h; Its optimum culturing temperature is 30 ℃, and the most suitable growth pH is neutrality or weakly alkaline.This bacterium can be respectively 100 mgL in the mass concentration of naphthalene and anthracene -1and 50mgL -1inorganic salt liquid substratum in well growth, with other microorganism comparisons of reporting, BDP01 has stronger degrading polycyclic aromatic hydrocarbons ability within a short period of time, the naphthalene of degradable 89. 64% and 77. 21% anthracene in 1% inoculum size 15d.
One, the mensuration of the degradation capability of BDP01 bacterial strain to naphthalene, anthracene:
1, the drafting of typical curve: naphthalene, the anthracene solution of preparing respectively different concns, be placed in the quartz colorimetric utensil of 3mL, measure respectively the absorbance (naphthalene: 275nm under corresponding operation wavelength with ultraviolet spectrophotometer, anthracene: 254nm), take absorbance as ordinate zou, the concentration of naphthalene or anthracene is X-coordinate drawing standard curve.Wherein, naphthalene typical curve and anthracene typical curve are suc as formula shown in (1) and (2):
y?=0.?2722x?-0.?2916　R2?=0.?9982?(1)
y?=0.?1999x?-0.?2183　R2?=0.?9986?(2)。
2, degradation capability is measured:
1. preparing naphthalene concentration is 100 mgL -1, anthracene concentration is 50 mgL -1inorganic salt liquid substratum: (NH 4) SO 43g, KH 2pO 40.5g, Na 2hPO 40.5g, MgSO 40.3g, trace element solution 1mL, adds naphthalene or the anthracene of respective quality, adjusts pH=7.0, with ddH 2o is settled to 1000mL, and 121 ℃ of sterilizing 20min are standby.
Wherein, being formulated as of trace element solution: FeCl 30. 3 g, FeSO 47H 2o 0. 3 g, MnSO 4h 2o 0.15 g, ZnSO 40.14 g, CoCl 20.2 g, with ddH 2o is settled to 1000mL, standby after filtration sterilization.
2. the BDP01 bacterium of logarithmic phase is accessed in inorganic salt liquid substratum to 35 ℃, 150rmin by 1% inoculum size -1lower constant-temperature shaking culture.Every 72h sampling 1 time in culturing process, whole bottle extracts nutrient solution, uses organic solvent (naphthalene normal hexane, anthracene hexanaphthene) oscillation extraction 3 times, and extraction liquid is analyzed with ultraviolet spectrophotometer after constant volume.Detect the light absorption value under corresponding wavelength (naphthalene: 275nm, anthracene: 254nm), according to typical curve, calculate its degradation rate (Wen et al., 2005; Zhang et al., 2004), 3 repetitions are done in experiment.
The applicant utilizes Auele Specific Primer to increase to BDP01 bacterial strain, amplifying size is the Catechol 2 of 400 bp left and right, 3-dioxygenase (C23O) gene fragment, C23O DNA homolog in its sequence and ncbi database is up to 92%, from gene level, has confirmed that BDP01 bacterial strain has the hereditary basis of degrading polyaromatic hydrocarbon.This enzyme is at 20 ~ 65 ℃ during take pyrocatechol as substrate, and in cell pyrolysis liquid, enzyme activity variation range is 13. 10-540. 86 U.
Two, inoculum size is on the degrade impact of naphthalene, anthracene of BDP01
Inoculum size is degraded the impact of naphthalene, anthracene as Fig. 5 and 6 to BDP01.As we know from the figure, inoculum size is on the BDP01 impact of naphthalene, anthracene little of degrading, and 0.5,1,1.5,2% inoculum size 15d is very close to the degradation rate of naphthalene, anthracene, but the degradation rate maximum of 1% inoculum size to naphthalene generally speaking reaches 89.64%; The degradation rate maximum of 1.5% inoculum size to anthracene, reaches 77.21%.
Three, different pH, temperature, inoculum size, tween 80 content to the Degrading experiment effect of anthracene referring to table 4.
The Orthogonal experiment results of table 4 BDP01 degraded anthracene
。
From Fig. 5,6 and table 4:
1. BDP01 has stronger degradation capability to naphthalene, anthracene, and inoculum size is little on the impact of its degradation rate, all in all, and the naphthalene of 1% inoculum size 15d degradable 89.64%, the anthracene of 1.5% inoculum size 15d degradable 77.21%.
2. can significantly promote BDP01 degradation capability appropriate adding of Tween80, add 0.5% Tween80 the ability of BDP01 15d degraded naphthalene can be brought up to 96.132% in substratum, the ability of degraded anthracene brings up to 89.916%.But experimental result equally also shows, adds too much Tween80 can reduce on the contrary the efficiency of BDP01 degraded naphthalene, anthracene.
3. known by orthogonal experiment, pH is very remarkable on the impact of BDP01 degraded naphthalene, and tween 80 impact is for remarkable, and temperature and inoculum size impact are not remarkable.Tween80 is very remarkable on the impact of BDP01 degraded anthracene, and significantly, inoculum size impact is not remarkable for pH value and temperature impact.
It is good to the treatment effect of sewage that above-mentioned experiment further illustrates complex microbial inoculum of the present invention.
Finally it should be noted that: the foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, although the present invention is had been described in detail with reference to previous embodiment, for a person skilled in the art, its technical scheme that still can record aforementioned each embodiment is modified, or part technical characterictic is wherein equal to replacement.Within the spirit and principles in the present invention all, any modification of doing, be equal to replacement, improvement etc., within all should being included in protection scope of the present invention.

Claims (6)

1. a complex microbial inoculum is in the application of processing in petrifaction sewage mud.
2. application according to claim 1, is characterized in that: the step of described application is as follows:
(1) prepare bio-carrier: in polyphenylene oxide, add glutaraldehyde to suspend and stir, after washing, add containing CaCl 2pBS damping fluid standing;
(2) enlarged culturing of complex microbial inoculum;
(3) complex microbial inoculum after enlarged culturing step (2) being obtained is put into bio-carrier prepared by step (1);
(4) bio-carrier that is loaded with complex microbial inoculum step (3) being obtained adds in petrifaction sewage, complex microbial inoculum continued to rise in value to stationary phase, on bio-carrier surface, form microbial film, carry out metabolism, organic pollutant and heavy metal in absorption, absorption, digestion, decomposition petrifaction sewage and mud, make it to transform into stable innoxious material.
3. application according to claim 2, it is characterized in that: enlarged culturing described in step (2) is that complex microbial inoculum is coated in LB solid medium, shaking culture 24-48 hour on 20-30 ℃, the shaking table of 150 rpm/min, to complex microbial inoculum concentration be 10 3individual/ml.
4. application according to claim 3, is characterized in that: the temperature of described shaking culture is 30 ℃.
5. application according to claim 2, it is characterized in that: the complex microbial inoculum after the enlarged culturing in step (3), step (2) being obtained is put into after bio-carrier prepared by step (1), at 20-25 ℃, stir 30min, 4 ℃ standing 48 hours.
6. application according to claim 5, is characterized in that: the temperature of described stirring is 20 ℃.
CN201310742337.5A 2013-12-30 2013-12-30 Application of compound microorganism bacterium agent in treatment of petrochemical sewage and sludge Withdrawn CN103755039A (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CN201310742337.5A CN103755039A (en) 2013-12-30 2013-12-30 Application of compound microorganism bacterium agent in treatment of petrochemical sewage and sludge
CN201410578342.1A CN104310594B (en) 2013-12-30 2014-10-24 The application of a kind of complex microbial inoculum in process petrifaction sewage mud

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201310742337.5A CN103755039A (en) 2013-12-30 2013-12-30 Application of compound microorganism bacterium agent in treatment of petrochemical sewage and sludge

Publications (1)

Publication Number Publication Date
CN103755039A true CN103755039A (en) 2014-04-30

Family

ID=50522406

Family Applications (2)

Application Number Title Priority Date Filing Date
CN201310742337.5A Withdrawn CN103755039A (en) 2013-12-30 2013-12-30 Application of compound microorganism bacterium agent in treatment of petrochemical sewage and sludge
CN201410578342.1A Expired - Fee Related CN104310594B (en) 2013-12-30 2014-10-24 The application of a kind of complex microbial inoculum in process petrifaction sewage mud

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN201410578342.1A Expired - Fee Related CN104310594B (en) 2013-12-30 2014-10-24 The application of a kind of complex microbial inoculum in process petrifaction sewage mud

Country Status (1)

Country Link
CN (2) CN103755039A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103787508A (en) * 2014-01-16 2014-05-14 兰州石化职业技术学院 Application of microbial compound agent in treatment of petrochemical sewage and sludge
CN105861366A (en) * 2016-04-19 2016-08-17 南京大学 Application of polycyclic aromatic hydrocarbon degrading strain FA1 in absorption of heavy metal Pb ions

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105000674B (en) * 2015-07-04 2017-02-15 郭晓燕 Method used for processing environmental geology industrial wastewater via biotechnology
CN107935339B (en) * 2017-10-27 2018-09-21 河海大学 A kind of aquaculture pond bed mud heavy metal stabilizer, its production method and its application
CN109734203B (en) * 2019-03-15 2021-11-26 东北农业大学 Method for removing heavy metals in water body by combining humin and microorganisms and repairing filler
CN110980960B (en) * 2019-11-26 2022-04-08 浙江永续环境工程有限公司 Oil removing microbial inoculum and sludge treatment method using same

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2067083C1 (en) * 1994-04-22 1996-09-27 Научно-производственное предприятие "Экобиос" Method for water treatment
US6503746B1 (en) * 1998-08-17 2003-01-07 Rutgers, The State University Biologically pure Paenibacillus validus bacterial strains that degrade polyaromatic hydrocarbons
CN101186877A (en) * 2007-12-13 2008-05-28 哈尔滨工业大学 Biological treatment agent for petrochemical waste water
CN103342412B (en) * 2013-08-02 2015-04-08 武汉钢铁(集团)公司 Coke particle biomembrane carrier and preparation method thereof

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103787508A (en) * 2014-01-16 2014-05-14 兰州石化职业技术学院 Application of microbial compound agent in treatment of petrochemical sewage and sludge
CN105861366A (en) * 2016-04-19 2016-08-17 南京大学 Application of polycyclic aromatic hydrocarbon degrading strain FA1 in absorption of heavy metal Pb ions
CN105861366B (en) * 2016-04-19 2019-06-14 南京大学 Application of the polycyclic aromatic hydrocarbon-degrading bacteria FA1 in absorption heavy metal Pb ion

Also Published As

Publication number Publication date
CN104310594A (en) 2015-01-28
CN104310594B (en) 2016-04-27

Similar Documents

Publication Publication Date Title
CN104310594B (en) The application of a kind of complex microbial inoculum in process petrifaction sewage mud
Vyrides et al. Are compatible solutes compatible with biological treatment of saline wastewater? Batch and continuous studies using submerged anaerobic membrane bioreactors (SAMBRs)
Jin et al. Performance of an Anammox UASB reactor at high load and low ambient temperature
Welander et al. Denitrification at low temperatures using a suspended carrier biofilm process
Ji et al. Effect of different preservation conditions on the reactivation performance of anammox sludge
CN101397545B (en) O-nitrobenzaldehyde degrading bacteria and use thereof
Zhou et al. Potential effects of loading nano zero valent iron discharged on membrane fouling in an anoxic/oxic membrane bioreactor
CN111117912B (en) Salt-tolerant denitrifying bacteria strain, salt-tolerant denitrifying bacteria liquid, screening method and application
CN102586160B (en) Stenotrophomonas maltophilia DS4
CN103382049B (en) Sequencing batch activated sludge reactor for sewage treatment and treatment process
Hao et al. Resilience of sulfate-reducing granular sludge against temperature, pH, oxygen, nitrite, and free nitrous acid
CN102020359B (en) Method for removing cadmium and dichlorophenol in wastewater simultaneously by utilizing P.chrysosporium
Chabaud et al. Bacteria removal in septic effluent: Influence of biofilm and protozoa
CN104560823B (en) Can degrade acetonitrile Shewanella putrefaciens and its application
CN102776140B (en) Cold-tolerant pseudomonas strain Den-05, and screening method and application thereof
CN102583780A (en) Application of Stenotrophomonas maltophilia DS4 for degrading organic pollutants in saponin waste water
CN101811779B (en) Preparation method of halophilic decontamination bacterial agent and bacterial agent prepared by same
CN111139198B (en) Lactobacillus parvum GBW-HB1903 and application thereof
Zeng et al. Characterization of phenol degradation by high-efficiency binary mixed culture
CN103787508A (en) Application of microbial compound agent in treatment of petrochemical sewage and sludge
Ward et al. Sediment distribution of methanogenic bacteria in Lake Erie and Cleveland Harbor
Arcangeli et al. Growth of an aerobic and an anoxic toluene-degrading biofilm-a comparative study
CN103614324B (en) Short-chain fatty acid degradation bacteria and application thereof
CN202968242U (en) Sequencing batch activated sludge reactor for sewage treatment
CN101811780A (en) Preparation method and application of halophilic decontamination bacterial agent

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C04 Withdrawal of patent application after publication (patent law 2001)
WW01 Invention patent application withdrawn after publication

Application publication date: 20140430