CN109734203B - Method for removing heavy metals in water body by combining humin and microorganisms and repairing filler - Google Patents

Method for removing heavy metals in water body by combining humin and microorganisms and repairing filler Download PDF

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CN109734203B
CN109734203B CN201910197647.0A CN201910197647A CN109734203B CN 109734203 B CN109734203 B CN 109734203B CN 201910197647 A CN201910197647 A CN 201910197647A CN 109734203 B CN109734203 B CN 109734203B
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filler
water body
liquid
repairing
microorganisms
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CN109734203A (en
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赵越
张旭
魏自民
高昕童
解新宇
张闯
杨红宇
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Northeast Agricultural University
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Northeast Agricultural University
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Abstract

The invention discloses a method for removing heavy metals in a water body by combining humins and microorganisms, and provides a simple and recyclable method with excellent removal of heavy metals in the water body and a used repairing filler. The method comprises the following steps: firstly, mixing a liquid culture medium, microorganisms and humins to prepare a repairing liquid; secondly, the filler is placed into the repairing liquid for soaking, and then the filler is taken out and placed into the water body containing the heavy metal, so that the heavy metal in the water body can be removed. Repairing the filler: comprises liquid culture medium, microorganism and humins. The method has the advantages of flexible putting position and time when the heavy metal polluted water body is repaired, capability of changing at any time, simple method and low cost, and can be used for repairing the dynamic water body. The repairing filler can be recycled after being eluted, the eluent is convenient to collect, and the risk of secondary pollution is further reduced.

Description

Method for removing heavy metals in water body by combining humin and microorganisms and repairing filler
Technical Field
The invention relates to a method for removing heavy metals in a water body and repairing a filler.
Background
The rapid development of industrialization in recent years has made environmental issues, particularly water pollution, increasingly severe. Among them, heavy metal pollution is one of the most important factors causing water pollution. Heavy metal pollution in water refers to the pollution of pollutants containing heavy metal ions to the water body. Heavy metal wastewater (containing heavy metal ions such as chromium, cadmium, copper, mercury, nickel, zinc and the like) generated in industrial production processes such as mining and metallurgy, mechanical manufacturing, chemical industry, electronics, instruments and the like is one of the industrial wastewater which has the most serious pollution to water and the greatest harm to human beings.
The sludge containing heavy metal ions is used as a fertilizer, and the wastewater containing the heavy metal ions irrigates farmlands, so that the soil and underground water sources are polluted, and the heavy metal ions of crops exceed the standard; and heavy metals in the water body can be enriched in aquatic organisms and finally enter human bodies through food chains to cause serious harm. The Japanese public nuisance, water guarantee disease and pain disease in the world shocked in the 60 th century are caused by the pollution of water body with mercury-containing waste water and cadmium-containing waste water respectively.
Disclosure of Invention
The invention aims to provide a simple and recyclable method with excellent removal of heavy metals in water and a used repairing filler.
The method for removing the heavy metals in the water body by combining the humins and the microorganisms comprises the following steps:
firstly, mixing a liquid culture medium, microorganisms and humins to prepare a repairing liquid;
secondly, the filler is placed into the repairing liquid for soaking, and then the filler is taken out and placed into the water body containing the heavy metal, so that the heavy metal in the water body can be removed;
wherein, the microorganism in the first step is a strain or a flora capable of converting heavy metal ions into a low-toxicity state or a non-toxicity state;
the liquid medium is a medium suitable for the growth of the microorganism of step one.
Preferably, the liquid medium is LB liquid medium.
The humin in the repair liquid provides a habitat for microorganisms to adsorb heavy metals, and the number of microorganisms on the surface of the filler is increased; and the humins also carry a certain amount of liquid culture medium, so that sufficient nutrition is provided for the microorganisms in the transition period of adapting to the water body, and the heavy metal removal effect is achieved more quickly and better. The metabolite of the microorganism used in the invention enhances the bonding strength between the humin and the filler, enhances the impact resistance of the humin, greatly reduces the loss amount, and ensures that the excellent removal effect of heavy metal ions can be maintained for a long time.
The method has the advantages of flexible putting position and time when the heavy metal polluted water body is repaired, capability of changing at any time, simple method and low cost, and can be used for repairing the dynamic water body.
The repairing filler can be recycled after being eluted, the eluent is convenient to collect, and the risk of secondary pollution is further reduced.
Detailed Description
The technical solution of the present invention is not limited to the following specific embodiments, but includes any combination of the specific embodiments.
The first embodiment is as follows: the method for removing the heavy metals in the water body by combining the humins and the microorganisms in the embodiment comprises the following steps:
firstly, mixing a liquid culture medium, microorganisms and humins to prepare a repairing liquid;
secondly, the filler is placed into the repairing liquid for soaking, and then the filler is taken out and placed into the water body containing the heavy metal, so that the heavy metal in the water body can be removed;
wherein, the microorganism in the first step is a strain or a flora capable of converting heavy metal ions into a low-toxicity state or a non-toxicity state;
the liquid culture medium is a culture medium suitable for the growth of the microorganism in the step one;
the repair liquid is prepared according to the proportion of 20 plus or minus 2ml of liquid culture medium, 5 plus or minus 0.5ml of microorganism and 10 plus or minus 1mg of humin.
The second embodiment is as follows: the present embodiment differs from the first embodiment in that: the concentration of the microorganism is 106~108cfu/ml. Other steps and parameters are the same as those in the first embodiment.
The third concrete implementation mode: the present embodiment is different from the first or second embodiment in that: the microorganism is a liquid compound microorganism bacterium agent 1 (the liquid compound microorganism bacterium agent 1 is formed by mixing aspergillus niger fungus and pseudomonas according to the volume ratio of 1:1, and the concentration of the aspergillus niger fungus liquid is 106~108cfu/ml, concentration of pseudomonas liquid is 106~108cfu/ml), Bacillus cereus, Bacillus licheniformis, Bacillus thuringiensis and/or Pseudomonas nitroreducens. Other steps and parameters are the same as in one or both embodiments.
The microorganism according to the present embodiment may be a single strain or a complex microbial preparation of a plurality of strains.
The fourth concrete implementation mode: the present embodiment differs from the first embodiment in that: and (3) putting the filler into the repairing liquid for soaking for 0.5-1 h. Other steps and parameters are the same as those in the first embodiment.
The fifth concrete implementation mode: the present embodiment is different from one of the first to fourth embodiments in that: the filler is put into a static water body containing heavy metals for 4-10 days, and then the heavy metals in the water body can be effectively removed. The other steps and parameters are the same as those in one of the first to fourth embodiments.
The sixth specific implementation mode: the present embodiment is different from one of the first to fifth embodiments in that: and (3) putting the repairing filler into the dynamic water body, wherein the flow velocity of the water body is controlled to be below 0.1m/s, and the arrangement length of the repairing filler along the water flow direction is not less than 500 meters. Other steps and parameters are the same as those in one of the first to fifth embodiments.
The embodiment has more ideal effect of adopting discontinuous closure repair.
The seventh embodiment: the present embodiment differs from one of the first to sixth embodiments in that: the filler is put into a water body containing heavy metals for 20-50 days and taken out, and the filler can be recycled after elution. Other steps and parameters are the same as those in one of the first to sixth embodiments.
The specific implementation mode is eight: the repairing filler of the embodiment comprises a liquid culture medium, microorganisms and humins.
The specific implementation method nine: the present embodiment is different from the eighth embodiment in that: the preparation method of the repair filler comprises the following steps:
firstly, mixing a liquid culture medium, microorganisms and humins to prepare a repairing liquid;
and secondly, placing the filler into the repairing liquid to be soaked for 0.5-1 h to obtain the repairing filler. The other steps and parameters are the same as those in embodiment eight.
The detailed implementation mode is ten: the eighth embodiment differs from the ninth embodiment in that: the repair liquid is prepared according to the proportion of 20 plus or minus 2ml of liquid culture medium, 5 plus or minus 0.5ml of microorganism and 10 plus or minus 1mg of humin;
the concentration of the microorganism is 106~108cfu/ml;
The microorganism is a strain or a flora capable of converting heavy metal ions into a low-toxicity state or a non-toxicity state;
the liquid medium is a medium suitable for the growth of the microorganism of step one.
Example 1
Experimental group 1 (T1): firstly, mixing a liquid culture medium, microorganisms and humins to prepare a repairing liquid;
secondly, soaking the filler in the repairing liquid, and adsorbing 5ml (soaking for 0.5-1 h) of the repairing liquid by the filler to obtain an experimental filler T1;
the repair liquid is prepared by 20ml of liquid culture medium, 5ml of microorganism and 10mg of humin;
the microorganism is a liquid compound microorganism bacterium agent 1, and the concentration of the liquid compound microorganism bacterium agent 1 is 106~108cfu/ml; the liquid culture medium is LB liquid culture medium.
Experimental group 2 (T2): firstly, mixing a liquid culture medium, microorganisms and humins to prepare a repairing liquid;
secondly, soaking the filler in the repairing liquid, and adsorbing 5ml (soaking for 0.5-1 h) of the repairing liquid by the filler to obtain an experimental filler T2;
the repair liquid is prepared by 20ml of liquid culture medium, 5ml of microorganism and 10mg of humin;
the microorganism is a mixed solution of a liquid compound microorganism bacterium agent 1 and bacillus licheniformis R08 (wherein the volume ratio of the liquid compound microorganism bacterium agent 1 to the bacillus licheniformis R08 bacterium solution is 2:1), and the concentration of the microorganism bacterium solution is 106~108cfu/ml; the liquid culture medium is LB liquid culture medium.
Experimental group 3 (T3): and (3) soaking the filler in an LB liquid culture medium, and adsorbing 5ml (soaking for 0.5-1 h) of the LB liquid culture medium by using the filler to obtain the experimental filler T3.
Experimental group 4 (T4): and (3) soaking the filler in a mixed solution A of an LB liquid culture medium and the liquid compound microbial agent 1, and adsorbing the mixed solution A5ml by the filler (soaking for 0.5-1 h) to obtain the experimental filler T4.
Wherein the volume ratio of the LB liquid culture medium to the liquid compound microbial agent 1 in the mixed solution A is 4:1, and the concentration of the liquid compound microbial agent 1 is 106~108cfu/ml。
Experimental group 5 (T5): and (3) soaking the filler in a mixed solution B of an LB liquid culture medium and a microbial liquid, and adsorbing 5ml (soaking for 0.5-1 h) of the mixed solution B by the filler to obtain an experimental filler T5.
Wherein the volume ratio of the LB liquid culture medium to the microbial liquid in the mixed liquid B is 4: 1; the microbial liquid is a mixed liquid of a liquid compound microbial agent 1 and bacillus licheniformis R08 (wherein the volume ratio of the liquid compound microbial agent 1 to the bacillus licheniformis R08 bacterial liquid is 2:1), and the concentration of the microbial liquid is 106~108cfu/ml。
Experimental group 6 (T6): and (3) soaking the filler in a mixed solution C of an LB liquid culture medium and humins, and adsorbing 5ml (soaking for 0.5-1 h) of the mixed solution C by the filler to obtain an experimental filler T6.
Wherein the volume ratio of the LB liquid culture medium to the humins in the mixed solution C is 5: 1.
Experimental group 7 (T7): and (3) soaking the filler in a mixed solution D of the liquid compound microbial agent 1 and the humin, and adsorbing 5ml (soaking for 0.5-1 h) of the mixed solution D by the filler to obtain an experimental filler T7.
Wherein the volume ratio of the liquid compound microbial agent 1 to the humins in the mixed solution D is 1: 2.
The experimental method comprises the following steps:
preparing a water body to be restored: the concentrations of Zn, Cu, Ni, Pb, Cr and Cd in the water body to be restored are 7mg/L, 9mg/L, 7mg/L, 5mg/L, 8mg/L and 0.4mg/L respectively.
And (3) putting the experimental filler of each experimental group into 25ml of water to be restored for 7 days, taking out the filler, and detecting the residual amount of heavy metals in the water (shown in table 1).
TABLE 1 (unit: mg/L)
Zn Cu Ni Pb Cr Cd
Water body to be restored 9 7 7 5 8 0.4
T1 4.53 3.31 4.54 1.96 3.57 0.217
T2 3.21 2.72 4.47 0.83 2.86 0.137
T3 9 7 7 5 8 0.4
T4 7.47 6.87 6.02 2.75 6.12 0.288
T5 7.01 6.09 5.73 3.37 7.01 0.248
T6 6.31 3.97 5,46 3.07 4.85 0.296
T7 5.05 3.74 5.04 2.31 4.64 0.262

Claims (9)

1. A method for removing heavy metals in water by combining humins and microorganisms is characterized by comprising the following steps:
firstly, mixing a liquid culture medium, microorganisms and humins to prepare a repairing liquid;
secondly, the filler is placed into the repairing liquid for soaking, and then the filler is taken out and placed into the water body containing the heavy metal, so that the heavy metal in the water body can be removed;
wherein, the microorganism in the first step is a strain or a flora capable of converting heavy metal ions into a low-toxicity state or a non-toxicity state;
the liquid medium is a medium suitable for the growth of the microorganism of step one.
2. The method for removing heavy metals in water body by combining humins and microorganisms as claimed in claim 1, wherein the repairing solution is prepared by mixing 20 ± 2ml of liquid culture medium, 5 ± 0.5ml of microorganisms and 10 ± 1mg of humins.
3. The method for removing heavy metals from a water body by combining humins with microorganisms according to claim 2, wherein the concentration of the microorganisms is 106~108cfu/ml。
4. The method for removing heavy metals in water body by combining humins and microorganisms as claimed in claim 1, wherein the filler is placed in the repairing liquid for soaking for 0.5-1 h.
5. The method for removing the heavy metals in the water body by combining humins and microorganisms as claimed in claim 1, wherein the filler is put into the static water body containing the heavy metals for 4-10 days, so that the heavy metals in the water body are effectively removed.
6. The method for removing the heavy metals in the water body by combining humins and microorganisms as claimed in claim 1, wherein the filler is put into the water body containing the heavy metals for 20-50 days and then taken out, and the filler can be recycled after elution.
7. A filler for use in remediation of a water body as claimed in claim 1, wherein the remediation filler for removal of heavy metals from a water body comprises a liquid medium, microorganisms and humins.
8. The filler for water body restoration according to claim 1, wherein the filler for water body restoration is prepared by the following steps:
firstly, mixing a liquid culture medium, microorganisms and humins to prepare a repairing liquid;
and secondly, placing the filler into the repairing liquid to be soaked for 0.5-1 h to obtain the repairing filler.
9. The filling material for repairing water body in claim 1, according to claim 8, wherein the repairing liquid is prepared by the ratio of 20 ± 2ml of liquid culture medium, 5 ± 0.5ml of microorganism and 10 ± 1mg of humin;
the concentration of the microorganism is 106~108cfu/ml;
The microorganism is a strain or a flora capable of converting heavy metal ions into a low-toxicity state or a non-toxicity state;
the liquid medium is a medium suitable for the growth of the microorganism of step one.
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CN110951492B (en) * 2019-11-19 2021-04-27 西北农林科技大学 Preparation method and application of soil remediation medicament humin based on modified peat
CN116179529B (en) * 2023-02-24 2023-10-31 生态环境部华南环境科学研究所(生态环境部生态环境应急研究所) Method for degrading TBBPA by composting humus microbial agent and application

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104310594A (en) * 2013-12-30 2015-01-28 兰州石化职业技术学院 Application of microbial compound bacteria agent in treatment of petrochemical wastewater and sludge
CN105523643A (en) * 2015-12-25 2016-04-27 成都新柯力化工科技有限公司 Sewage treatment composite gel material for microbe embedding, and preparation method thereof
CN105713617A (en) * 2016-03-02 2016-06-29 广东省生态环境与土壤研究所(广东省土壤科学博物馆) Heavy metal cadmium passivator for activating activity of rice field soil sulfur reducing bacteria and application
CN106047850A (en) * 2016-05-24 2016-10-26 浙江清华长三角研究院 Microbe immobilizing agent as well as preparation method and applications thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104310594A (en) * 2013-12-30 2015-01-28 兰州石化职业技术学院 Application of microbial compound bacteria agent in treatment of petrochemical wastewater and sludge
CN105523643A (en) * 2015-12-25 2016-04-27 成都新柯力化工科技有限公司 Sewage treatment composite gel material for microbe embedding, and preparation method thereof
CN105713617A (en) * 2016-03-02 2016-06-29 广东省生态环境与土壤研究所(广东省土壤科学博物馆) Heavy metal cadmium passivator for activating activity of rice field soil sulfur reducing bacteria and application
CN106047850A (en) * 2016-05-24 2016-10-26 浙江清华长三角研究院 Microbe immobilizing agent as well as preparation method and applications thereof

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