CN109734203A - A kind of method and reparation filler of humin and microbial association removal Heavy Metals in Waters - Google Patents
A kind of method and reparation filler of humin and microbial association removal Heavy Metals in Waters Download PDFInfo
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- CN109734203A CN109734203A CN201910197647.0A CN201910197647A CN109734203A CN 109734203 A CN109734203 A CN 109734203A CN 201910197647 A CN201910197647 A CN 201910197647A CN 109734203 A CN109734203 A CN 109734203A
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Abstract
A kind of method of humin and microbial association removal Heavy Metals in Waters, the present invention provide a kind of simple, reusable, heavy metal in water and remove excellent method and used reparation filler.Method: one, fluid nutrient medium, microorganism and humin are mixed, reparation liquid is made；Two, filler is put into repair in liquid and is impregnated, then taken out and be put into the water body containing heavy metal, the removal of Heavy Metals in Waters can be realized.It repairs filler: including fluid nutrient medium, microorganism and humin.The position and time that the method for the present invention is put into repairing heavy metal pollution water body are flexible and can change at any time, and can be used for the reparation of dynamic water body, and method is simple, at low cost.Present invention reparation filler filler after eluting is reusable, and eluent also further reduced the risk of secondary pollution convenient for collecting.
Description
Technical field
The present invention relates to a kind of methods for removing heavy metal in water to repair filler.
Background technique
Industrialized fast development in recent years makes environmental problem especially water pollution problems increasingly serious.Wherein, a huge sum of money
Belonging to pollution is to cause one of most important factor of water pollution.Heavy Metals in Waters pollution refers to the pollutant containing heavy metal ion
It is polluted caused by water body into water body.The weight generated in the industrial processes such as mining and metallurgy, machine-building, chemical industry, electronics, instrument
Metallic wastewater (containing the heavy metal ion such as chromium, cadmium, copper, mercury, nickel, zinc) is to endanger to water pollution most serious and to the mankind maximum
One of industrial wastewater.
Waste water irrigation farmland of the sludge for containing heavy metal ion as fertilizer, containing heavy metal ion is used, will lead to soil
The pollution of earth and underground water source causes crop heavy metal ion exceeded；And the heavy metal in water body also can be in aquatile
Middle enrichment enters human body eventually by food chain and generates serious harm.The 1960s world-shaking Japanese public hazards
Disease --- minamata disease and itai-itai are exactly respectively as caused by mercury-containing waste water and cadmium wastewater polluted-water.
Summary of the invention
Excellent method is removed the object of the present invention is to provide a kind of simple, reusable, heavy metal in water and is made
Reparation filler.
The method of humin and microbial association removal Heavy Metals in Waters sequentially includes the following steps:
One, fluid nutrient medium, microorganism and humin are mixed, reparation liquid is made；
Two, filler is put into repair in liquid and is impregnated, then taken out and be put into the water body containing heavy metal, water body can be realized
The removal of middle heavy metal；
Wherein, microorganism is the bacterial strain or flora that can convert heavy metal ion to less toxic state or nontoxic state in step 1；
The fluid nutrient medium is the culture medium for being suitble to the growth of step 1 microorganism.
Preferably, fluid nutrient medium is LB liquid medium.
The humin that the present invention repairs in liquid provides habitat for microorganism adsorption heavy metal, and it is micro- to increase filler surface
Biomass；And humin also carries a certain amount of fluid nutrient medium, provides and fills for the transitional period that microorganism adapts to water body
The nutrition of foot, to faster, preferably reach heavy metals removal effect.The metabolite of microorganism used therefor of the present invention enhances recklessly
It is quick element and filler between bond strength, humin impact resistance enhancing, number of dropouts substantially measure reduction, ensure that heavy metal from
The excellent removal effect of son is kept for a long time.
The position and time that the method for the present invention is put into repairing heavy metal pollution water body are flexible and can change at any time, and
It can be used for the reparation of dynamic water body, method is simple, at low cost.
Present invention reparation filler filler after eluting is reusable, and eluent also further reduced two convenient for collecting
The risk of secondary pollution.
Specific embodiment
The technical solution of the present invention is not limited to the following list, further includes between each specific embodiment
Any combination.
Specific embodiment 1: the method for a kind of humin of present embodiment and microbial association removal Heavy Metals in Waters
It sequentially includes the following steps:
One, fluid nutrient medium, microorganism and humin are mixed, reparation liquid is made；
Two, filler is put into repair in liquid and is impregnated, then taken out and be put into the water body containing heavy metal, water body can be realized
The removal of middle heavy metal；
Wherein, microorganism is the bacterial strain or flora that can convert heavy metal ion to less toxic state or nontoxic state in step 1；
The fluid nutrient medium is the culture medium for being suitble to the growth of step 1 microorganism；
The liquid of repairing is prepared in the ratio of 20 ± 2ml fluid nutrient medium, 5 ± 0.5ml microorganism and 10 ± 1mg humin
It forms.
Specific embodiment 2: the difference of present embodiment and specific embodiment one is: the concentration of the microorganism
It is 106~108cfu/ml.Other steps and parameter are identical as embodiment one.
Specific embodiment 3: the difference of present embodiment and specific embodiment one or two is: the microorganism
For liquid composite microbial microbial inoculum 1, (liquid composite microbial microbial inoculum 1 is pressed by Aspergillus niger fungus and pseudomonad the volume ratio of 1:1
It mixes, the concentration of Aspergillus niger fungus bacterium solution is 106~108Cfu/ml, the concentration of pseudomonad bacterium solution are 106~
108Cfu/ml), Bacillus cercus, bacillus licheniformis, bacillus thuringiensis and/or Pseudomonas nitroreducens.It is other
Step and parameter are identical as embodiment one or two.
Microorganism described in present embodiment can be the composite bacteria agent of single bacterial strain or a variety of bacterial strains.
Specific embodiment 4: the difference of present embodiment and specific embodiment one is: filler, which is put into, to be repaired in liquid
Soaking time is 0.5~1h.Other steps and parameter are identical as embodiment one.
Specific embodiment 5: the difference of present embodiment and one of specific embodiment one to four is: filler is put into
4~10 days in static water body containing heavy metal, the heavy metal in water body can be effectively removed.Other steps and parameter and implementation
One of mode one to four is identical.
Specific embodiment 6: the difference of present embodiment and one of specific embodiment one to five is: to Dynamic Water
It is launched in body and repairs filler, water volume flow rate should be controlled in 0.1m/s to be not less than hereinafter, repairing filler along water (flow) direction and laying length
500 meters.Other steps and parameter are identical as one of embodiment one to five.
Present embodiment is even more ideal using the discontinuity repairing effect that shuts off.
Specific embodiment 7: the difference of present embodiment and one of specific embodiment one to six is: filler is put into
It takes out within 20~50 days in water body containing heavy metal, filler is reusable after elution.Other steps and parameter and embodiment
One of one to six is identical.
Specific embodiment 8: it includes fluid nutrient medium, microorganism and humin in filler that present embodiment, which is repaired,.
Specific embodiment 9: the difference of present embodiment and specific embodiment eight is: the system for repairing filler
Preparation Method are as follows:
One, fluid nutrient medium, microorganism and humin are mixed, reparation liquid is made；
Two, filler is put into repair and impregnates 0.5~1h in liquid, that is, obtain the filler of reparation.Other steps and parameter with
Embodiment eight is identical.
Specific embodiment 10: the difference of present embodiment and specific embodiment eight or nine is: the reparation liquid is pressed
The ratio of 20 ± 2ml fluid nutrient medium, 5 ± 0.5ml microorganism and 10 ± 1mg humin is formulated；
The concentration of the microorganism is 106~108cfu/ml；
Microorganism is the bacterial strain or flora that can convert heavy metal ion to less toxic state or nontoxic state；
The fluid nutrient medium is the culture medium for being suitble to the growth of step 1 microorganism.
Embodiment 1
Experimental group 1 (T1): one, fluid nutrient medium, microorganism and humin are mixed, reparation liquid is made；
Two, filler is put into repair in liquid and is impregnated, filling adsorption repairs liquid 5ml (impregnating is 0.5~1h) to get experiment is arrived
Filler T1；
The reparation liquid is formulated in the ratio of 20ml fluid nutrient medium, 5ml microorganism and 10mg humin；
The microorganism is liquid composite microbial microbial inoculum 1, and the concentration of liquid composite microbial microbial inoculum 1 is 106~
108cfu/ml；Fluid nutrient medium is LB liquid medium.
Experimental group 2 (T2): one, fluid nutrient medium, microorganism and humin are mixed, reparation liquid is made；
Two, filler is put into repair in liquid and is impregnated, filling adsorption repairs liquid 5ml (impregnating is 0.5~1h) to get experiment is arrived
Filler T2；
The reparation liquid is formulated in the ratio of 20ml fluid nutrient medium, 5ml microorganism and 10mg humin；
The microorganism is liquid composite microbial microbial inoculum 1 and bacillus licheniformis R08 (wherein liquid composite microbial bacterium
The volume ratio of agent 1 and bacillus licheniformis R08 bacterium solution is 2:1) mixed liquor, the concentration of microbial inoculum is 106~108cfu/ml；
Fluid nutrient medium is LB liquid medium.
Experimental group 3 (T3): filler being put into LB liquid medium and is impregnated, and filling adsorption LB liquid medium 5ml (impregnates
For 0.5~1h) to get to experiment filler T3.
Experimental group 4 (T4): filler is put into LB liquid medium and the mixed liquor A of liquid composite microbial microbial inoculum 1 and is soaked
Bubble, mixed liquor A 5ml described in filling adsorption (impregnating is 0.5~1h) is to get to experiment filler T4.
Wherein, LB liquid medium and the volume ratio of liquid composite microbial microbial inoculum 1 are 4:1, liquid in the mixed liquor A
The concentration of complex micro organism fungicide 1 is 106~108cfu/ml。
Experimental group 5 (T5): filler being put into LB liquid medium and the mixed liquid B of microbial inoculum and is impregnated, and filler is inhaled
The attached mixed liquid B 5ml (impregnating is 0.5~1h) is to get to experiment filler T5.
Wherein, LB liquid medium and the volume ratio of microbial inoculum are 4:1 in the mixed liquid B；The microorganism
Bacterium solution is liquid composite microbial microbial inoculum 1 and bacillus licheniformis R08 (wherein liquid composite microbial microbial inoculum 1 and lichens gemma
The volume ratio of bacillus R08 bacterium solution is 2:1) mixed liquor, the concentration of microbial inoculum is 106~108cfu/ml。
Experimental group 6 (T6): filler being put into the mixed liquor C of LB liquid medium and humin and impregnated, filling adsorption institute
The mixed liquor C 5ml (impregnate be 0.5~1h) stated is to get to experiment filler T6.
Wherein, LB liquid medium and the volume ratio of humin are 5:1 in mixed liquor C.
Experimental group 7 (T7): filler being put into the mixed liquor D of liquid composite microbial microbial inoculum 1 and humin and impregnated, filler
The absorption mixed liquor D 5ml (impregnating is 0.5~1h) is to get to experiment filler T7.
Wherein, the volume ratio of liquid composite microbial microbial inoculum 1 and humin is 1:2 in mixed liquor D.
Experimental method:
Prepare water body to be repaired: in water body to be repaired the concentration of Zn, Cu, Ni, Pb, Cr and Cd be respectively 7mg/L, 9mg/L,
7mg/L, 5mg/L, 8mg/L and 0.4mg/L.
Each experimental group experiment filler is put into the water body to be repaired that volume is 25ml and is handled 7 days, filler is then taken out, examines
Survey the residual volume (as shown in table 1) of Heavy Metals in Waters.
Table 1 (unit: mg/L)
| Zn | Cu | Ni | Pb | Cr | Cd | |
| Water body to be repaired | 9 | 7 | 7 | 5 | 8 | 0.4 |
| T1 | 4.53 | 3.31 | 4.54 | 1.96 | 3.57 | 0.217 |
| T2 | 3.21 | 2.72 | 4.47 | 0.83 | 2.86 | 0.137 |
| T3 | 9 | 7 | 7 | 5 | 8 | 0.4 |
| T4 | 7.47 | 6.87 | 6.02 | 2.75 | 6.12 | 0.288 |
| T5 | 7.01 | 6.09 | 5.73 | 3.37 | 7.01 | 0.248 |
| T6 | 6.31 | 3.97 | 5,46 | 3.07 | 4.85 | 0.296 |
| T7 | 5.05 | 3.74 | 5.04 | 2.31 | 4.64 | 0.262 |
Claims (9)
1. a kind of method of humin and microbial association removal Heavy Metals in Waters, it is characterised in that the removal heavy metal in water
Method sequentially include the following steps:
One, fluid nutrient medium, microorganism and humin are mixed, reparation liquid is made；
Two, filler is put into repair in liquid and is impregnated, then taken out and be put into the water body containing heavy metal, weight in water body can be realized
The removal of metal；
Wherein, microorganism is the bacterial strain or flora that can convert heavy metal ion to less toxic state or nontoxic state in step 1；
The fluid nutrient medium is the culture medium for being suitble to the growth of step 1 microorganism.
2. the method for a kind of humin according to claim 1 and microbial association removal Heavy Metals in Waters, feature
Be it is described reparation liquid in 20 ± 2ml fluid nutrient medium, 5 ± 0.5ml microorganism and 10 ± 1mg humin ratio prepare and
At.
3. the method for a kind of humin according to claim 2 and microbial association removal Heavy Metals in Waters, feature
The concentration for being the microorganism is 106~108cfu/ml。
4. the method for a kind of humin according to claim 1 and microbial association removal Heavy Metals in Waters, feature
Being that filler is put into soaking time in reparation liquid is 0.5~1h.
5. the method for a kind of humin according to claim 1 and microbial association removal Heavy Metals in Waters, feature
It is to be put into filler in the static water body containing heavy metal 4~10 days, effectively removes the heavy metal in water body.
6. the method for a kind of humin according to claim 1 and microbial association removal Heavy Metals in Waters, feature
Be filler be put into the water body containing heavy metal take out within 20~50 days, filler is reusable after elution.
7. repairing filler as described in claim 1, it is characterised in that remove in the reparation filler of heavy metal in water comprising liquid
Culture medium, microorganism and humin.
8. reparation filler according to claim 7, it is characterised in that it is described repair filler the preparation method comprises the following steps:
One, fluid nutrient medium, microorganism and humin are mixed, reparation liquid is made；
Two, filler is put into repair and impregnates 0.5~1h in liquid, that is, obtain the filler of reparation.
9. reparation filler according to claim 8, it is characterised in that the reparation liquid presses 20 ± 2ml fluid nutrient medium, 5
The ratio of ± 0.5ml microorganism and 10 ± 1mg humin is formulated；
The concentration of the microorganism is 106~108cfu/ml；
Microorganism is the bacterial strain or flora that can convert heavy metal ion to less toxic state or nontoxic state；
The fluid nutrient medium is the culture medium for being suitble to the growth of step 1 microorganism.
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| CN201910197647.0A CN109734203B (en) | 2019-03-15 | 2019-03-15 | Method for removing heavy metals in water body by combining humin and microorganisms and repairing filler |
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Cited By (2)
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| CN110951492A (en) * | 2019-11-19 | 2020-04-03 | 西北农林科技大学 | Preparation method and application of soil remediation medicament humin based on modified peat |
| CN116179529A (en) * | 2023-02-24 | 2023-05-30 | 生态环境部华南环境科学研究所(生态环境部生态环境应急研究所) | Method for degrading TBBPA by composting humus microbial agent and application |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110951492A (en) * | 2019-11-19 | 2020-04-03 | 西北农林科技大学 | Preparation method and application of soil remediation medicament humin based on modified peat |
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| CN116179529B (en) * | 2023-02-24 | 2023-10-31 | 生态环境部华南环境科学研究所(生态环境部生态环境应急研究所) | Method for degrading TBBPA by composting humus microbial agent and application |
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