CN106635855A - Microbacterium kitamiense and culture application thereof - Google Patents
Microbacterium kitamiense and culture application thereof Download PDFInfo
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Abstract
The invention relates to microbacterium kitamiense and culture application thereof. The strain is microbacterium kitamiense FSTB-4 and is preserved in China General Microbiological Culture Collection Center (CGMCC) on June 1, 2015; and the preservation number is CGMCC No.10939. The strain can be applied to efficient removal of COD (Chemical Oxygen Demand) in salt-containing wastewater with the salt content of 1.0wt%-5.0wt%. The invention further provides a salt-tolerant bacterium agent for removing the COD and the bacterium agent comprises the microbacterium kitamiense FSTB-4. The microbacterium kitamiense provided by the invention can be used for rapidly degrading and removing the COD in the high-salt-content wastewater and has the characteristics of high salt-tolerant capability, good treatment effect and the like.
Description
Technical field
The invention belongs to field of environment microorganism, and in particular to microbacterium and its culture application are seen in a kind of de- COD north of salt tolerant, the bacterial strain is particularly well-suited to the efficient removal of COD in high slat-containing wastewater.
Background technology
Generally contain higher COD from the high slat-containing wastewater that the salt content of the industries such as oil, chemical industry, medicine and chemical fertilizer is higher than 1%, Pollutants in Wastewater is constantly discharged in environment can cause serious pollution problem.The shortage problem of freshwater resources is increasingly serious, and the process and its recycling of waste water are more subject to people's attention.
Physically or chemically method processes the COD in high slat-containing wastewater, high cost and is likely to result in secondary pollution.Traditional biological method has great advantage when Low-salinity waste water is processed, but in high salt, high soda acid, high temperature or low temperature inverse ring border, the degradation capability of microorganism will be suppressed.Facultative Halophiles process high slat-containing wastewater and have the unrivaled advantage of conventional activated sludge process, and than physical-chemical process low cost, can produce more obvious economic benefit and social benefit, can provide technical support for related wastewater treatment qualified discharge.
Facultative Halophiles technology is based on the problem of engineering field and is badly in need of and produces, and by scientific method the advantage Facultative Halophiles of process high slat-containing wastewater are tamed out, and the bacteroid can be grown with its unique eucaryotic cell structure and material composition in the environment of higher salinity.The abundant enzyme system and activated product that the salt-tolerance character of such salt-durable microbe adapts to extreme environment with it is relevant.
In high slat-containing wastewater process field, salt content is a key factor for affecting microbiological treatment waste water efficiency.Believe glad etc.（Biological reinforcing technology processes highly salt containing organic waste water [J]. water technology, 2008,8：66-70）Highly salt containing organic waste water is processed using biological reinforcing technology, significantly improved using the dehydrogenase activity of the biological reinforced saponin waste water biological treatment system activated sludge of Facultative Halophiles, when system tolerance chlorine ion concentration is up to 2.8%, the clearance of saponin waste water COD is 84.41%.CN201210130657.0, CN201210130644.3, CN201010536065.X and CN201210130658.5 are related to using marsh cock Salmonella（Kocuri apalustris）FSDN-A, Staphylococcus cohnis（Staphylococcus cohnii）FSDN-C, arthrobacterium（Arthrobacter creatinolyticus）FDN-1, Shui Shi Flavobacterium（Flavobacterium mizutaii）FDN-2, Paracoccus denitrificans（Paracoccus denitrificans）DN-3 and Methylobacterium（Methylobacterium phyllosphaerae）The method that SDN-3 is carried out a biological disposal upon to the ammonia nitrogen in brine waste and catalytic cracking catalyst waste water, total nitrogen and COD.The method needs to cultivate various bacterial strains and carries out compounding and prepares salt tolerant microbial inoculum, and preparation process is complicated, and production cost is higher.
At present, the relevant report that microbacterium is seen in the north for processing high slat-containing wastewater is not also screened.
The content of the invention
It is an object of the invention to provide microbacterium and its culture application are seen in a kind of de- COD north of salt tolerant.The north that the present invention is provided sees that microbacterium can remove the COD in high slat-containing wastewater with fast degradation, strong with salt resistance ability, the features such as high treating effect.
The present invention provides a kind of north and sees microbacterium FSTB-4, and its Classification And Nomenclature is shown in microbacterium for north（Microbacterium kitamiense）, it is preserved in " China Committee for Culture Collection of Microorganisms's common micro-organisms center " on June 1st, 2015, deposit number is CGMCCNo.10939.
Microbacterium FSTB-4 is seen in the north that the present invention is provided, and main morphological features are：Colony colour is shallower taupe brown, and it is shaft-like that bacterial strain is individual.Physiological and biochemical property shows as：Gram-positive, oxidase negative contacts enzyme positive, and decomposable asymmetric choice net utilizes several kinds of carbon source, with nitrate reduction characteristic.The bacterial strain is resistant to AZT or acidum nalidixicum.
The north that the present invention is provided sees that the 16SrRNA gene orders of microbacterium FSTB-4 are shown in sequence table.
The north that the present invention is provided sees that microbacterium FSTB-4 removes the application in COD in brine waste.The bacterial strain can be applied to the efficient removal of COD in the brine waste of salt content 1.0wt%-5.0wt%.
In the present invention, the brine waste can be the strong brine that the waste water of the generations such as chemical industry synthesis waste water, coal chemical industrial waste water, or oil refining process, Coal Chemical Industry technique and chemical industry synthesis technique through pre-processing is produced through counter-infiltration minimizing unit.Described brine waste salt content is 0.5wt%-5.0wt%, preferably 1.0wt%-4.5wt%, COD（Cr methods, similarly hereinafter）Content is 200-20000mg/L.
The cultural method of microbacterium FSTB-4 is seen in the north that the present invention is provided, including the three phases such as bacterial strain activation, liquid seeds liquid culture, aeration culture, concretely comprises the following steps：
（1）Bacterial strain is activated：North is seen into that microbacterium FSTB-4 is inoculated in the inclined-plane of FSTB solid mediums or flat board, 25-35 DEG C is cultivated 48 hours, in being stored in 4 DEG C of refrigerators.
（2）Liquid seeds liquid culture：Prepare FSTB fluid nutrient mediums, in being sub-packed in triangular flask, sterilize and be cooled to after room temperature, the inoculation activated in picking inclined-plane or flat board under gnotobasis in triangular flask, 25-35 DEG C of culture 24-72h.The FSTB fluid nutrient mediums are：FeSO4•7H2O
25mg/L, NH4NO3286mg/L, KCl 929mg/L, CaCl22769mg/L, NaCl 21008mg/L, beef extract 5g/L, peptone 10g/L, pH value range is 6.0-8.5, preferably 6.5-8.0.FSTB solid mediums are addition 20g/L agar in liquid medium within.
（3）Aeration culture：FSTB fluid nutrient mediums or pending brine waste are added in the reactor for be provided with aerator, ratio according to volume ratio 5%-25% is inoculated with liquid seed liquor, pH value is controlled in 6.0-8.5, aeration culture 48-96 hours, periodic feed supplement and discharge operation are carried out afterwards, withdrawal rate accounts for the 5%-90% of reactor volume, feed supplement amount accounts for the 5%-90% of reactor volume, also a small amount of carbon source can be added, nitrogen source and microelement substance, culture 24-48 hours are 1 cultivation cycle, discharge the nutrient solution of corresponding volume according to aforementioned proportion afterwards, thus obtain seeing the dense bacterium solution product of microbacterium containing higher concentration north.
In the present invention, after culture terminates, the bacterium solution that collection culture is finished, during the flat board containing FSTB solid mediums is applied to after dilution, the bacterium colony to growing is counted and is counted the percentage of bacterium colony similar to FSTB-4.Under closed culture environment, north sees that microbacterium is pure bacterial strain, and percent similarity reaches more than 95%.The growing environment of culture FSTB-4 is although the high salt selection pressure with continuation, but other a small amount of interference strain growths are still might have in long term open formula culture, to prevent from disturbing bacterium continuation flourish to become dominant bacteria, when percent similarity it is close 50% when, the mode of inoculated and cultured of intaking again after whole discharges can be taken, normal open culture operating process is recovered afterwards.
The present invention also provides a kind of salt tolerant microbial inoculum of de- COD, and the microbial inoculum includes that microbacterium FSTB-4 is seen in north.Described salt tolerant microbial inoculum can see salt tolerant microbial inoculum obtained in microbacterium FSTB-4 bacterium solutions only with north, can also be with other salt tolerant microbial inoculum compoundings, such as can be combined with the existing microbial bacterial agent with denitrogenation, dephosphorization and degraded hardly degraded organic substance, be realized more preferable water treatment effect.
The salt tolerant north that the present invention is provided sees that microbacterium FSTB-4 is one plant of bacterial strain that in Halite water body there is preferable COD to remove performance, salt resistance ability is strong, and high treating effect is used directly for the process of brine waste, during existing brine waste processing system can also be added to, water treatment effect is improved.The cultural method of microbacterium FSTB-4 is seen in the north that the present invention is provided, and can directly utilize sewage treatment plant's brine waste（A small amount of natural medium and chemical synthesis culture medium are added according to condition of water quality）Dominant strain culture enrichment enrichment work is carried out, the growth characteristics and resistance characteristics of salt tolerant microbial inoculum are greatly maintained, and reduces the toxigenic capacity of salt tolerant microbial inoculum.
Biomaterial preservation explanation
Microbacterium is seen in the north that the present invention is provided（Microbacterium kitamiense）FSTB-4 bacterial strains, are preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center（CGMCC）；Address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Institute of Microorganism, Academia Sinica；Deposit number：CGMCC
No.10939；Preservation date：On June 1st, 2015.
Specific embodiment
The north of the present invention sees that microbacterium FSTB-4 is in June, 2014 and obtains for separation screening in the activated sludge for processing epoxychloropropane production waste water from taking from Hunan Yueyang petroleum chemical enterprise that its physiological and biochemical test the results are shown in Table 1.
The physiological and biochemical test result of microbacterium FSTB-4 is seen in the north of table 1
Jing " China Committee for Culture Collection of Microorganisms's common micro-organisms center " identifies that the bacterial strain belongs to north and sees microbacterium（Microbacterium kitamiense）, it is named as FSTB-4.
Embodiment 2 is screened and isolated and purified
Domestication culture 15 days is carried out to the activated sludge for taking from Hunan Yueyang petroleum chemical enterprise for processing epoxychloropropane production waste water using the method for gradually stepping up nutrient solution salinity.
The good activated sludge of above-mentioned domestication is taken, initial gross separation is carried out in enriched medium using dilution spread method, thus obtain 15 bacterium colonies that growth conditions are good and proterties is different.The main component that the enriched medium for being adopted contains for：COD is 1000mg/L, and calcium ion concentration is 1000mg/L, and total salt quantity is 10000mg/L, and agar content is for 2.0% in the solid medium for being adopted.
Then the bacterium colony for obtaining is transferred using plate streaking method, line is transferred in screening and culturing medium, is further separated and is screened.The main component that the screening and culturing medium for adopting contains for：COD is 1000mg/L, and calcium ion concentration is 4000mg/L, and total salt quantity is 25000mg/L, and agar content is for 2.0% in the solid medium for being adopted.
10 plants of pure bacterial strains are obtained by aforesaid operations, wherein one plant is north and sees microbacterium FSTB-4.
The investigation of microbacterium FSTB-4 salt resistance abilities is seen in the north of embodiment 3
North of the present invention sees that the cultural method of microbacterium FSTB-4 includes：Bacterial strain activation, liquid seeds liquid culture, aeration culture, detailed process is as follows：
（1）Bacterial strain is activated：North is seen into that microbacterium FSTB-4 is inoculated in the flat board containing FSTB solid mediums, is cultivated 48 hours under 30 DEG C of environment, be then stored in stand-by in 4 DEG C of refrigerators.The FSTB solid mediums are：FeSO4•7H2O 25mg/L, NH4NO3
286mg/L, KCl
929mg/L, CaCl22769mg/L, NaCl 21008mg/L, beef extract 5g/L, peptone 10g/L, agar 20g/L, pH value 7.8.
（2）Liquid seeds liquid culture：FSTB fluid nutrient mediums are prepared, in being sub-packed in triangular flask, is sterilized and is cooled to after room temperature, cultivated 48 hours under the conditions of 30 DEG C in triangular flask with the inoculation after activation in oese picking flat board under gnotobasis.The FSTB fluid nutrient mediums are：FeSO4•7H2O
25mg/L, NH4NO3286mg/L, KCl 929mg/L, CaCl22769mg/L, NaCl 21008mg/L, beef extract 5g/L, peptone 10g/L, pH value 7.8.
（3）Aeration culture：Cultivated using closed reactor，It is complete that institute is required to sterilizing using reactor and various utensils，Air inlet and exhaust position need to install bacteriological filter apparatus，Nutrient solution、Acid-base modifier and trace element solution are required to be added according to sterile working code after sterilizing，Reactor need to be equipped with aerator，And can carry out into water、Acid adjustment、Alkali tune、Feed supplement and draining discharge are operated，The FSTB fluid nutrient mediums being put in the reactor after sterilizing，Ratio according to reactor volume percentage 10% is inoculated with liquid seed liquor，After opening incubation，Adopt soda acid automatic control system by medium pH value scope control in 6.0-8.5 in incubation，Aeration culture carries out afterwards periodic feed supplement and discharge operation for 72 hours，Discharge is the nutrient solution of reactor volume percentage 50%，Feed supplement is the FSTB fluid nutrient mediums of reactor volume percentage 50%，Also a small amount of carbon source can simultaneously be added、Nitrogen source and microelement substance，Culture 24 hours is 1 cultivation cycle，Discharge the nutrient solution of corresponding volume according to aforementioned proportion afterwards，Thus obtain seeing the dense bacterium solution product of microbacterium containing pure north.
Gather the dense bacterium solution that above-mentioned culture is finished, during the flat board containing FSTB solid mediums is applied to after dilution, bacterium colony to growing is counted and is counted the percentage of bacterium colony similar to FSTB-4, and percent similarity reaches more than 95%, that is, assert that the north sees that microbacterium is pure bacterial strain.
The north obtained using above-mentioned culture sees that microbacterium FSTB-4 processes brine waste.By taking the epichlorohydrin production process brine waste through pretreatment as an example, the water quality of handled brine waste is the present embodiment：COD concentration ranges are 800-2000mg/L, and salt content is 1wt%-5wt%, and treatment temperature is 30 DEG C.Treatment effect is as shown in table 2.
The north of table 2 sees that microbacterium FSTB-4 processes the effect of brine waste
As shown in Table 2, north sees that the salt content that microbacterium FSTB-4 is resistant to is 1.0wt%-5.0wt%, can be with the COD in efficient removal waste water.
The open cultures of microbacterium FSTB-4 are seen in the north of embodiment 4
North of the present invention sees that the open culturing method of microbacterium FSTB-4 includes：Bacterial strain activation, liquid seeds liquid culture, open aeration culture, the detection of pure bacterial strain and dominant bacteria safeguard that detailed process is as follows：
（1）Bacterial strain is activated：North is seen into that microbacterium FSTB-4 is inoculated in the flat board containing FSTB solid mediums, is cultivated 48 hours under 30 DEG C of environment, be then stored in stand-by in 4 DEG C of refrigerators.The FSTB solid mediums are：FeSO4•7H2O 25mg/L, NH4NO3
286mg/L, KCl
929mg/L, CaCl22769mg/L, NaCl 21008mg/L, beef extract 5g/L, peptone 10g/L, agar 20g/L, pH value 7.8.
（2）Liquid seeds liquid culture：FSTB fluid nutrient mediums are prepared, in being sub-packed in triangular flask, is sterilized and is cooled to after room temperature, cultivated 48 hours under the conditions of 30 DEG C in triangular flask with the inoculation after activation in oese picking flat board under gnotobasis.The FSTB fluid nutrient mediums are：FeSO4•7H2O
25mg/L, NH4NO3286mg/L, KCl 929mg/L, CaCl22769mg/L, NaCl 21008mg/L, beef extract 5g/L, peptone 10g/L, pH value 7.8.
（3）Open culture：Pending brine waste is added in the open reactive device for being provided with aerator, by taking the epichlorohydrin production process brine waste through pretreatment as an example, the water quality of handled brine waste is the present embodiment：COD concentration ranges are 1000mg/L, and salt content is 1wt%-5.0wt%, and cultivation temperature is 35 DEG C, and according to the ratio of volume ratio 10% liquid seed liquor, aeration culture 96 hours, the change of nutrient solution cell concentration are inoculated with（With absorption values OD during 600nm600Represent cell concentration）As shown in table 3.
The north of table 3 sees that microbacterium FSTB-4 cell concentrations change
（4）Pure bacterial strain detection：Gather the bacteria agent mixed liquor that above-mentioned culture is finished, during the flat board containing FSTB solid mediums is applied to after dilution, bacterium colony to growing is counted and is counted the percentage of bacterium colony similar to FSTB-4, and percent similarity reaches more than 50%, then assert that north sees that microbacterium is dominant strain.
（5）Dominant strain is safeguarded：The pure bacterial strain detection similar bacterium colony percentages of FSTB-4 it is close 50% when, take water inlet and the again mode of inoculated and cultured again after whole discharges, normally open culture flow process is recovered afterwards.
North of the present invention sees that microbacterium FSTB-4 is very good to the adaptability and treatment effect of actual brine waste, with stronger COD degradation function, individually the process of brine waste can be used for using the pure culture of the bacterial strain, also can amplify be added to existing process flow process as external source functional microorganism after culture, targetedly regulation is optimized to actual process situation, solves the problems, such as active sludge treatment effect difference and easily degeneration under hypersaline environment.High slat-containing wastewater is processed than physical-chemical process low cost, direct application can produce more obvious economic benefit and social benefit, can provide technical support for high saliferous correlation wastewater treatment qualified discharge using Facultative Halophiles.
SEQUENCE
LISTING
<110>
Sinopec Group
Dalian Petroleum Chemical Engineering Institute of Sinopec Group
<120>
Microbacterium and its culture application are seen in a kind of north
<130>
New application
<170> PatentIn version 3.5
<211> 1304
<212> DNA
<213> Microbacterium
aacgggtgag taacacgtga
gcaacctgcc ctgaactctg ggataagcgc tggaaacggc
60
gtctaatact ggatatgagc
tgcggaggca tcttcgtggt tggaaagatt tttcggtttg
120
ggatgggctc gcggcctatc
agcttgttgg tgaggtaatg gctcaccaag gcgtcgacgg
180
gtagccggcc tgagagggtg
accggccaca ctgggactga gacacggccc agactcctac
240
gggaggcagc agtggggaat
attgcacaat gggcggaagc ctgatgcagc aacgccgcgt
300
gagggatgac ggccttcggg
ttgtaaacct cttttagcag ggaagaagcg agagtgacgg
360
tacctgcaga aaaagcgccg
gctaactacg tgccagcagc cgcggtaata cgtagggcgc
420
aagcgttatc cggaattatt
gggcgtaaag agctcgtagg cggtttgtcg cgtctgctgt
480
gaaatctggg ggctcaaccc
ccagcctgca gtgggtacgg gcagactaga gtgcggtagg
540
ggagattgga attcctggtg
tagcggtgga atgcgcagat atcaggagga acaccgatgg
600
cgaaggcaga tctctgggcc
gtaactgacg ctgaggagcg aaagggtggg gagcaaacag
660
gcttagatac cctggtagtc
caccccgtaa acgttgggaa ctagttgtgg ggtccattcc
720
acggattccg tgacgcagct
aacgcattaa gttccccgcc tggggagtac ggccgcaagg
780
ctaaaactca aaggaattga
cggggacccg cacaagcggc ggagcatgcg gattaattcg
840
atgcaacgcg aagaacctta
ccaaggcttg acatacacga gaacgctgca gaaatgtaga
900
actctttgga cactcgtgaa
caggtggtgc atggttgtcg tcagctcgtg tcgtgagatg
960
ttgggttaag tcccgcaacg
agcgcaaccc tcgttctatg ttgccagcac gtaatggtgg
1020
gactcatggg atactgccgg
ggtcactcgg aggaaggtgg ggatgacgtc aaatcatcat
1080
gccccttatg tcttgggctt
cacgcatgct acaatggccg gtacaaaggg ctgcgatacc
1140
gcgaggtgga gcgaatccca
aaaagccggt cccagttcgg attgaggtct gcaactcgac
1200
ctcatgaagt cggagtcgct
agtaatcgca gatcagcaac gctgcggtga atacgttccc
1260
gggtcttgta cacaccgccc
gtcaagtcat gaaagtcggt aaca
1304
Claims (10)
1. microbacterium FSTB-4 is seen in a kind of north, and its Classification And Nomenclature is shown in microbacterium for north（Microbacterium kitamiense）, it is preserved in " China Committee for Culture Collection of Microorganisms's common micro-organisms center " on June 1st, 2015, deposit number is CGMCC No.10939.
2. bacterial strain according to claim 1, it is characterised in that：The morphological feature of the bacterial strain is：Colony colour is shallower taupe brown, and it is shaft-like that bacterial strain is individual.
3. bacterial strain according to claim 1, it is characterised in that：The physiological and biochemical property of the bacterial strain is：Gram-positive, oxidase negative contacts enzyme positive, and decomposable asymmetric choice net utilizes several kinds of carbon source, with nitrate reduction characteristic；It is resistant to AZT or acidum nalidixicum.
4. north described in claim 1 sees that microbacterium FSTB-4 removes the application in COD in brine waste.
5. application according to claim 4, it is characterised in that：The bacterial strain can be applied to the efficient removal of COD in the brine waste of salt content 1.0wt%-5.0wt%.
6. the application according to claim 4 or 5, it is characterised in that：The brine waste is chemical industry synthesis waste water, the coal chemical industrial waste water through pre-processing, or the strong brine that the waste water of oil refining process, Coal Chemical Industry technique and chemical industry synthesis technique generation is produced through counter-infiltration minimizing unit, salt content is 0.5wt%-5.0wt%, and COD contents are 200-20000mg/L.
7. according to claim 1 the cultural method of microbacterium FSTB-4 is seen in north, it is characterised in that including bacterial strain activation, liquid seeds liquid culture, aeration culture three phases, concretely comprise the following steps：
（1）Bacterial strain is activated：North is seen into that microbacterium FSTB-4 is inoculated in the inclined-plane of FSTB solid mediums or flat board, 25-35 DEG C is cultivated 48 hours, in being stored in 4 DEG C of refrigerators；
（2）Liquid seeds liquid culture：Prepare FSTB fluid nutrient mediums, in being sub-packed in triangular flask, sterilize and be cooled to after room temperature, the inoculation activated in picking inclined-plane or flat board under gnotobasis in triangular flask, 25-35 DEG C of culture 24-72h；
（3）Aeration culture：FSTB fluid nutrient mediums or pending brine waste are added in the reactor for be provided with aerator, ratio according to volume ratio 5%-25% is inoculated with liquid seed liquor, pH value is controlled in 6.0-8.5, aeration culture 48-96 hours, periodic feed supplement and discharge operation are carried out afterwards, withdrawal rate accounts for the 5%-90% of reactor volume, feed supplement amount accounts for the 5%-90% of reactor volume, culture 24-48 hours are 1 cultivation cycle, discharge the nutrient solution of corresponding volume according to aforementioned proportion afterwards, obtain seeing the dense bacterium solution product of microbacterium containing higher concentration north.
8. method according to claim 7, it is characterised in that：The FSTB fluid nutrient mediums are：FeSO4•7H2O 25mg/L, NH4NO3
286mg/L, KCl 929mg/L, CaCl22769mg/L, NaCl 21008mg/L, beef extract 5g/L, peptone 10g/L, pH value is 6.0-8.5；FSTB solid mediums are addition 20g/L agar in liquid medium within.
9. method according to claim 7, it is characterised in that：In long term open formula culture, to prevent from disturbing bacterium continuation flourish to become dominant bacteria, collection culture finishes bacterium solution, during the flat board containing FSTB solid mediums is applied to after dilution, bacterium colony to growing is counted and is counted the percentage of bacterium colony similar to FSTB-4, when percent similarity it is close 50% when, the mode of inoculated and cultured of intaking again after whole discharges can be taken, normal open culture operating process is recovered afterwards.
10. a kind of salt tolerant microbial inoculum of de- COD, it is characterised in that：The microbial inoculum includes that microbacterium is seen in north（Microbacterium kitamiense）FSTB-4.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110229766A (en) * | 2019-06-14 | 2019-09-13 | 浙江工业大学 | Aoxidize microbacterium and its application in degradable organic pollutant |
| CN111348752A (en) * | 2018-12-21 | 2020-06-30 | 中国石油化工股份有限公司 | Treatment method of high-salt degradation-resistant wastewater |
| CN115960745A (en) * | 2022-07-26 | 2023-04-14 | 中国科学院天津工业生物技术研究所 | Salt-tolerant epichlorohydrin degrading bacterium and application thereof in treatment of epichlorohydrin in high-salinity water |
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| CN110229766B (en) * | 2019-06-14 | 2021-06-08 | 浙江工业大学 | Microbacterium oxydans and application thereof in degradation of organic pollutants |
| CN115960745A (en) * | 2022-07-26 | 2023-04-14 | 中国科学院天津工业生物技术研究所 | Salt-tolerant epichlorohydrin degrading bacterium and application thereof in treatment of epichlorohydrin in high-salinity water |
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