CN105851622A - Microelement premix for improving eggshell strength and preparing method thereof - Google Patents

Abstract

The invention relates to a microelement premix for improving the eggshell strength and a preparing method thereof. The microelement premix comprises, by weight, 1.5-2.5 parts of copper sulfate pentahydrate, 8-15 parts of ferrous sulfate monohydrate, 10-15 parts of manganese sulfate monohydrate, 5-8 parts of zinc sulphate monohydrate, 0.2-1 part of iodine, 0.1-1 part of selenium, 35-40 parts of mountain flour, 30-40 parts of medical stone and 1-5 parts of defatted rice bran. The preparing method includes the following steps of a, purifying and impurity removing; b, drying; c, smashing and sieving; d, pelletizing; e, anti-caking treating; f, diluting; g, raw material mixing. By means of the microelement premix, transport of calcium phosphorus and microelement to eggshell glands can be promoted, tissue reparation of organic-body organs and ovaries of egg chickens can be achieved, the elasticity of matrix protein fibers is enhanced, the compactness of the protein-ceramic structure of a palisade layer is improved, and the resistance of the surfaces of eggshells to fracture in the horizontal direction and the perpendicular direction is improved.

Description

A kind of microelement pre-mix material improving eggshell strength and preparation method thereof

Technical field

The invention belongs to field of fodder, be specifically related to a kind of microelement pre-mix material improving eggshell strength and Preparation method.

Background technology

Improve the necessity of eggshell strength

For adapting to modernization, scale, intensive production, automatization's egg picking equipment is reducing work because of it Intensity, improves work efficiency and avoids having in the cross infection caused when manually picking up egg irreplaceable effect, In egg circular flow from Producer to consumer, the egg of about 4.5%～10% is poor due to eggshell quality And bring direct economic loss to numerous raisers.Egg is after long-distance transport, and rate of broken eggs is even as high as 10%～15%.

Additionally, quality of egg not only affects qualification rate and the shelf life of commodity egg, have an effect on the incubation rate of hatching egg. Eggshell has important function for egg, and egg internal component can be protected from the invasion and attack of microorganism and environment Change and the infringement of external force；Eggshell has during hatching of breeding eggs resists microorganism invasion, at fetal development During exchanged by the gentle body of free surface moisture in pore regulation and control, and be that fetal development supplements when yolk calcium depletion The effects such as calcium element.Therefore, for meeting the needs of automatization's egg production, long-distance transport, trade and breeding, Seek a kind of effective way improving eggshell quality, for reducing the loss of egg fowl aquaculture and promoting that egg fowl produces Industry development is particularly important.

The formation of eggshell

The forming process of eggshell

Eggshell be followed successively by from inside to outside shell membrane, papillary layer, crystalline palisade layer, vertical crystal layer, uvea and Horny layer.Shell membrane is divided into inside and outside two-layer, the X collagen fabric mainly secreted by isthmus tubular gland cell Composition, shell membrane internal layer is entrenched togather with papillary layer each other without calcification, outer layer.At isthmus of uterine tube, nipple Body is formed at shell membrane surface.The surface being anchored to outer layer shell membrane that these mammillary bodyes are firm for starting calcium Deposition has important function.Mammillary body can form continuous print thin slice on egg shell membrane surface, is covered in whole shell membrane, This is papillary layer；Crystalline palisade layer is to be made up of the intensive calcium crystal of fence form, in uterus, and eggshell calcium That changes initial produces stromatin fiber based on mammillary body, and this substrate is through in eggshell crystallizing layer, at eggshell Forming process provide network structure to support for calcium salt crystallization, by controlling the size of crystal, shape and determining To the microstructure affecting eggshell.Stromatin fiber is generally oriented and is parallel to egg shell membrane surface, gives egg Shell elasticity and shock resistance.With the crystals of calcium salts that 96% calcium carbonate and a small amount of magnesium carbonate, tricalcium phosphate are crystal formation, Under the location that stromatin fibrous layer is suitable, constitute column fence structure.The fence structure that calcium crystal is constituted It is oriented perpendicular to egg eggshell surface and increases eggshell strength.Along with the increase of shell thickness, fence finally mixes Close and form an albumen-ceramic structure.The outermost layer of eggshell is vertical crystal layer.This is one and is oriented perpendicular to The fine and close calcium thin crystal layer on shell membrane surface, gives eggshell shell hardness and surface smoothness.Sinking of ooporphyrin The long-pending latter stage betiding doped calcium process.The difference of horny layer and outer calcification layer pigmentation ratio result in egg The white of shell or brown difference.

The formation of egg is a continuous print process, and in completing the overall process from ovulating to laying eggs, egg exists Formation time substantially 24h～25h in fallopian tube.Formed inside and outside after entering isthmus of uterine tube through 1～2h Shell membrane；Eggshell papillary layer is formed through 5h at tubulose eggshell gland；Enter eggshell glandular sac portion 15～16h Calcification forms eggshell.From the beginning of ovulation, the time that eggshell is formed accounts for whole egg and forms the 4/5 of the time, carbon Acid calcium crystal and eggshell stromatin interact, and chimeric formation has the eggshell of certain thickness and intensity.

Eggshell formed in key factor

1) transhipment of calcium and the factor of deposition are affected:

The forming process of eggshell is a calcification process in fact, about eggshell formed many studies have shown that, with Ca²⁺The correlation factor transported, absorb and deposit mainly has calbindin, carbonic anhydrase, Ca²⁺ATPase、 Epithelium calcium channel (TRPVs) etc..

Calbindin (CaBP-d28k):

CaBP-d28k is a member in calbindin family, the CaBP-d28k in poultry intestinal tract and eggshell gland Mainly secreted, at fowl small intestinal, kidney and eggshell gland etc. and Ca by intestinal secretion cell and tubular gland cell²⁺Inhale Receive expression in the closely-related tissue of transhipment higher, participate in small intestinal Ca²⁺Absorb, break bone process and eggshell gland Interior Ca²⁺Transhipment (Bar, 2009).

Carbonic anhydrase (CA):

CA is the zinc enzyme found in 1940, is widely present in mammal and birds body, its main merit Can be catalysis H₂CO₃Decompose, be H⁺+HCO₃ ^-=H₂CO₃The ingredient of reaction, CA is primarily involved in the weight of skeleton Absorb and calcification, ion transport, amino acid metabolism and breathing etc..Zinc relies on Ca and is catalyzed H₂CO₃, increase shell The available HCO of gland calcification₃ ^-, then become crystal calcium carbonate with calcium binding, it is deposited on egg shell membrane surface, Thus form eggshell.

Ca²⁺ATPase:

Ca²⁺Absorption, transhipment be all power consumption process, relevant to this process also have some energy " pump " and friendship Change system.Ca²⁺ATPase has more than 30 isozyme, is widely present in the small intestinal of mammal, kidney and tire In dish, major function is to utilize ATP by Ca²⁺Transporte to cells is outer to reduce cell potential poor (Bar, 2008), Immunohistochemical study shows, there is also Ca in laying hen eggshell gland²⁺ATPase, is positioned at eggshell glandular tube chamber cell apical On microvillose membrane, with Ca²⁺Transport relevant, if Ca²⁺ATPase activity inhibited, then ovary energy metabolism is by shadow Ring.

2) stroma fibrin

Eggshell be exactly calcium crystal with the interphase interaction of matrix fiber composition and the one that formed has certain thickness Degree, the material of intensity, matrix components rises actively in terms of regulation and control eggshell crystal habit and deposition dynamic behaviour Effect.Stromatin mainly affects eggshell by two kinds of mechanism and is formed: the most Sulfated proteoglycan passes through Electrostatic interaction each other affects mineralising；The phosphorylation of albumen, such as osteopontin, it relies on phosphoric acid The deposition of the form strong inhibition calcium carbonate changed.Research shows, the change of eggshell quality and eggshell stromatin Composition difference relevant (Nys et al, 2004).

Pontin protein (OPN):

OPN is the glycoprotein of a kind of hyperphosphorylation, by the synthesis of eggshell gland tubular epithelium and secretion, main Palisade layer to be present in, because post translational modification is different, molecular weight, 46～54kDa, has three kinds of principal modes, Can be by the orientation of crystal and calcification speed being regulated the growth of palisade layer.In calcification process, OPN can be in conjunction with To the surface of crystal growth, and along this surface modification resistance to fracture.OPN Various Tissues (bone, Eggshell gland etc.) middle existence, participate in Ca2+ transhipment, if this protein gene expression is abnormal, eggshell calcification can be given Bring defect.

OC-17 albumen:

OC-17 albumen is synthesized by tubulose eggshell gland in eggshell forming process, is present in whole eggshell calcification portion Position, is concentrated mainly in newborn weak and incompetent layer, is a kind of ovum functional protein.

Trace element effect in eggshell strength regulates and controls

Eggshell is mainly made up of inorganic matter, accounts for the 94%～97% of whole eggshell, and Organic substance accounts for eggshell 3%～6%.Inorganic matter mainly includes calcium carbonate (about 93%), magnesium carbonate (about 1%), calcium carbonate and carbonic acid Magnesium compound (about 2.8%), additionally, possibly together with various trace elements in eggshell, as zinc, copper, manganese, ferrum, Selenium etc..Outside deliming, phosphorus, utilization rate biology of other mineral element is the most out in the cold, especially zinc and Manganese.But, they are formed at carbonate and occupy very important in the enzyme system of protein-mucopolysaccharide synthesis Status.Micro-element nutrition occupies critical role in eggshell quality.Copper and manganese element shortage can affect eggshell Film formation, egg shape, shell thickness and egg yield.Manganese is most important to the glycoprotein constituting egg shell membrane, and egg Calcification during eggshell is formed by glycoprotein in shell structure and other organic substance has a major impact.

Zn effect in eggshell strength regulates and controls

1 antioxidation

Along with the increase of stressor in environment, laying hen is often subject to various stress stimulation, affects histoorgan Function and the health of laying hen and production performance, reduce economic benefit.Lay eggs the later stage, vigorous through the long period Lipid metabolism, the antioxidant status of laying hen body be more vulnerable to impact.Zn participates in kind more than 300 in body The synthesis of enzyme, regulation protein, lipid and the metabolism (Parkin, 2004) of carbohydrate, for maintaining DNA, RNA conformational stability and cell signalling process, the integrity of cell membrane and body in transcription Homergy play vital effect.

Antioxidation is one of critical function of zinc, and it can promote the formation of laying hen in-vivo metal sulfoprotein (MT), Free radical in purged body；It is the ingredient of Cu-Zn-SOD, improves SOD active, scavenging hydroxyl, Superoxide anion etc.；There is antagonism in transition metal such as ferrum, copper etc. that also can be active with oxidoreduction, presses down The catalysis activity of copper processed, ferrum etc., suppresses H₂O₂Form free radical with oxygen etc., delay internal oxidizing process.

2Zn and eggshell

Eggshell be by calcium carbonate and stroma fibrin in certain proportion, structure pile up and form, wherein CaCO₃ Accounting for more than 95%, matrix components accounts for 1%～3%, thus, the key factor during eggshell is formed mainly regulates CaCO₃ Deposition and the formation of matrix components.Eggshell is formed in eggshell gland, provides enough calcium ions for eggshell gland The most necessary.Meanwhile, eggshell gland should have the carbanion of abundance, in order to both combine formation calcium carbonate. One important sources of carbonic acid is carbon dioxide, and it is eggshell glandular cell homergy or blood circulation is provided 's.

Carbonic anhydrase with zinc as prothetic group plays important in carbon dioxide with the balancing response of water formation carbonic acid Catalytic action, that produce in cellular process or in blood gaseous mixture CO₂It is the main next of carbonate ions Source, carbonic anhydrase needs the participation of zinc catalytic water and carbon dioxide to form carbonic acid, and promotes H₂CO₃Hydrolysis, Improve HCO3 in eggshell gland^-Concentration, promote CaCO₃Deposition；Research finds, stopping production hen carbonic anhydrase Activity is less than bird inlay.When carbonic anhydrase activity is suppressed, then bicarbonate ion content reduces, and leads Cause Eggshell weight decline, also have research to point out, this type of trace element can change eggshell calcium crystal morphology and Mechanical strength feature (Mabe et al., 2003；Zamani et al, 2005).It is reported that, amino-acid zinc can Can help to eliminate hen aging brings eggshell quality to reduce effect (Swiatkiewicz& Koreleski, 2008), and can carry to aged hen (69-73 week and 69-82 week) supplementary zinc, manganese, copper High eggshell mechanical strength, it is strong with machinery that the reciprocal action of various trace elements is likely to be of in raising eggshell certain Acting on rather than the deposition (Mabe et al, 2003) of raising eggshell formation material requested of degree related substances content.

It is true that zinc is as the cofactor of this fermentoid so that zinc and the mineral relevant to cryptic enzyme structure It is bigger that element forms interactive probability.As, hen drinks the higher water of saliferous can cause carbonic anhydrase Activity reduces, and adds zinc and is favorably improved carbonic anhydrase activity, has confirmed, calcium and zinc, copper, manganese are mutual Compete one and absorb site, the therefore absorption to copper, manganese especially Zn of the high dietary calcium antagonism, reduce carbonic anhydride The activity of enzyme.Additionally, zinc is as the ingredient of alkali phosphatase (ALP), to some phosphorus in eggshell substrate Acidified protein (OPN, OC-116 etc.) plays certain regulating and controlling effect, thus affects carbonic acid directly or indirectly The formation of calcium crystal.

Mn effect in eggshell strength regulates and controls

Great majority research shows, zinc is only and manganese acts on competence exertion effect simultaneously, and manganese and zinc are in vivo Influencing each other during metabolism, Fe content is too high, suppresses the absorption of zinc.Leach and Gross (1983) finds After laying hen manganese deficiency, hence it is evident that reduce laying rate and shell thickness, the ultrastructure of eggshell especially mammillary body occurs Morphologic change, eggshell outward appearance is coarse.Leach and Gross (2003) reports, manganese deficiency state Under, laying hen eggshell mastoid process layer presents bigger irregular type mastoid process body, and in eggshell substrate, aminohexose is with own Glucuronic acid content declines.This research explanation manganese plays an important role in the building-up process of mucopolysaccharide, and eggshell Acid mucopolysaccharide content relevant to the breaking resistance of eggshell to a great extent.

In recent years, it has been found that manganese plays pivotal role in the activation process of glycosyl transferase, and likely It it is enzyme active center.Glycosyl transferase catalytic activation glycosyl in vivo is connected to different acceptor molecules, On albumen, nucleic acid, oligosaccharide, fat and little molecule, glycation product has various biological function.As Mucopolysaccharide and Dan Baiduotang proteoglycan PG in eggshell and shell membrane can regulate and control eggshell and be formed, and affects calcium carbonate crystal preferred orientation (Nakano etc., 2001,2002；Nys etc., 2004).Ouzzine etc. (2000) report, manganese is Galactose-β-1, the active factors of 3-glucuronyl transferase (GlcAT-I), can be by a glucose Aldehydic acid residue transfers to (Gal-β 1,3-Gal-β Isosorbide-5-Nitrae-Xyl-β on Dan Baiduotang proteoglycan PG (PG) chain -O-Ser), the synthesis of catalysis glycosaminoglycans, by GlcAT-I channel genes, can promote that PG synthesizes further (Venkatesan etc., 2004).Result of study shows recently, and in egg shell membrane, the content of GAG is strong with eggshell Degree has the strongest dependency (Young etc., 2007).Junfeng XIAOs etc. add 100mg/kg in layer diets Mn with without comparing, significantly improve the thickness of eggshell, intensity and toughness, significantly reduce mastoid process layer thickness With mastoid process width, hence it is evident that reduce eggshell surface crackle, significantly improve the table of glycosyltransferase gene in eggshell gland Reach (Junfeng XIAO, 2014), improve glycosaminoglycans and the content of alduronic acid in egg shell membrane.

Cu effect in eggshell strength regulates and controls

Scarce copper causes eggshell deformity (Baumgartner etc., 1978).Copper is many intracellular and extracellular Enzyme, such as the composition of cytochrome oxidase, lysyl oxidase, ceruloplasmin and superoxide dismutase Partly (Klasing, 1998), Chowdury etc. (1990) report lysyloxidase is a kind of cupric gold Belonging to enzyme, this enzyme catalysis lysine changes into desmosine, and (crosslinked formed by four lysines, sees elasticity Albumen) or isodensmosine, scarce copper causes lysine cross-links body structural change, causes fibre structure in shell membrane different Often, thus affect eggshell calcification.Many results of study prove, when layer diets copper exceedes pharmacological concentrations (＞ Time 250ppm), can be to period of sexual maturity laying hen carbohydrate, lipid, amino acid metabolism and 17 beta estradiols Level produces to be affected (Bakalli, 1995；Pesti, 1998).

OLGUN etc. (2012) study discovery, give sea blue brown layer diets containing 0～120ppm copper can't to B, Distribution and the deposition of Zn, Mn impact, even if (150～300ppm) are to bivalent ions at high doses Deposition only have minimal effect, it is indicated that copper can by disturb trace element metabolism, improve eggshell quality (proportion, Intensity, thickness, weight) effect, and the interpolation of boron significantly reduces shell thickness but have raising skeleton bullet The effect of property.

The impact on eggshell quality of the different trace element sources

After antibiotic is disabled, it is anti-that the growth-promoting effect of organic trace element makes it at home and abroad become replacement The common practice of raw element, organic trace is combined due to it with albumen or carbohydrate and reduces between trace element Antagonism, there is utilization ratio higher biology, but it still suffers from dispute to the impact of eggshell quality. Swiatkiewicz etc. (2008) use organic zinc, manganese progressively to replace Hai Lan with the gradient of 0,50,100% The ZnO of supplementary feeding in laying hens essential ration, after MnO, to performance in layers, shell thickness, weight, density All without impact, but eliminate, along with aging (62-70 week) of age in days has, the adverse effect that the age brings, improve The effect of eggshell strength；And Pekel etc. (2012) give Roman egghen feed three kinds of copper sources (Inorganic Copper, Aminoacid copper, protein copper) result of the test show, inorganic or organic copper to shell thickness, rate of broken eggs, Density all has no significant effect；Yenice etc. use containing two levels organic or inorganic Mn, Zn, Cu and Cr (80, 60、5、0.15mg/kg；40,30,2.5,0.07mg/kg) Diet laying hen also draw similar Conclusion, low concentration trace element significantly reduces eggshell strength, and separate sources trace element is to eggshell quality Have no significant effect (Yenice, 2015).Considering the factor of age in days and test period, organic trace is always Age hen has aged hen body aging is mitigated or eliminated, the work of negative effect that digestion and metabolism weakens With.Also it is reported that, Zn-Met combine other chelated microelements use to production performance and eggshell quality Without improvement result, and the Cu-Met of 100ppm improves production performance and eggshell strength, but has increased the weight of the flesh of chicken Stomach corrosion (Lim, 2003).

Brief summary

At present, many about being improved the report of eggshell quality by route of nutrition, focus primarily upon macroelement (Ca, P) and vitamin D₃Impact on eggshell quality.It is known that macroelement is in eggshell composition and dimension Hold and eggshell structure has important function, but trace element has considerable effect in eggshell quality. In recent years, researchers have done the much research improving eggshell quality, such as breeding, environment, nutrition especially The effort in mineral nutrition direction.Trace element can participate in eggshell and egg shell membrane by the catalytic action of key enzyme Formation, or with eggshell constitute in calcium crystal directly act on.Further, trace element such as Cu, Zn not only structure Become eggshell to form the active center of middle key enzyme, be also maintenance DNA, RNA structure in cell proliferation and injury repairing As stability and the important factor of cell membrane integrity；Cu, Se etc. are playing antioxidant activity, are participating in body Internal organs reparation, raising immunity and reproductive performance has important function.

The problem that the especially aged laying hen eggshell quality of laying hen eggshell strength declines is by extensive concern.Improve eggshell The measure of intensity, such as forced molting, improve calcium phosphorus and other nutrient concentration, strengthen feeding and management, Reduce egg size etc. and all can improve the eggshell quality decline that chicken group's aging brings to a certain extent, develop one Kind can promote that tissue repair and eggshell mineral nitrogen deposit, improve the Resisting fractre ability of eggshell crystal arrangement and shell membrane Balanced nutrients element, also provides the another kind of way for choice for this problem of solution.

Summary of the invention

It is an object of the invention to provide a kind of microelement pre-mix material improving eggshell strength and preparation method thereof, This microelement pre-mix material can promote that calcium phosphorus and trace element, to the transhipment of eggshell gland, contribute to laying hen body device Dirty and the tissue repair of ovary, strengthens stromatin fibrous elasticity, improves the cause of palisade layer albumen-ceramic structure Close property, improves the eggshell surface resistance both horizontally and vertically to fracture.

It is an object of the invention to be achieved through the following technical solutions:

A kind of microelement pre-mix material improving eggshell strength, including the raw material of following parts by weight: five water sulfur Acid copper 1.5～2.5 parts, ferrous sulfate monohydrate 8～15 parts, manganese sulfate monohydrate 10～15 parts, zinc sulfate monohydrate 5～8 Part, iodine 0.2～1 part, selenium 0.1～1 part, stone powder 35～40 parts, Maifanitum 30～40 parts and defatted rice bran 1～5 Part.

The preparation method of the described microelement pre-mix material improving eggshell strength, comprises the following steps:

A. purification and impurity removal: remove the impurity in raw material of the present invention, uses recrystallization method to remove anhydrous sulfur Exceed standard in acid copper arsenic；

B. it is dried: each raw material is carried out 30 DEG C of dried 24h；

C. pulverize and sieve: each raw material is sieved, it is desirable to 100% passes through 35 mesh sieves, and 90%～95% passes through 100 mesh sieves；

D. micronized: iodine and selenium are mixed into trace element mixture；

E. agglomeration resistance processes: with calcium stearate or Bentonite etc., copper sulphate pentahydrate is carried out agglomeration resistance process；

F. dilution processes: increase the free-running property of trace element mixture with stone powder；

G. raw material mixing: be sufficiently mixed by all raw materials, prepares the microelement pre-mix material improving eggshell strength.

The invention have the benefit that first, the present invention uses the processing technique of high-quality feedstocks and science, The biological value of trace element can be improved, reduce the antagonism between trace element, promote calcium phosphorus and trace unit Element is to the transhipment of eggshell gland.The second, micro-element nutrition of the present invention supply is precisely, and Zn, Mn, Cu, Se can Participate in removing free radical, suppression radical reaction, and promote superoxide dismutase, catalase, paddy Guang sweet peptide peroxidase etc. synthesize, and with VE, Vc collaborative promotion antioxidation, contribute to laying hen body device Dirty and the tissue repair of ovary.3rd, the present invention can promote the CaCO of eggshell₃In deposition and egg shell membrane, osamine gathers Sugar and the formation of alduronic acid, strengthen stromatin fibrous elasticity, improves the densification of palisade layer albumen-ceramic structure Property, improve the eggshell surface resistance both horizontally and vertically to fracture.

Detailed description of the invention

Below by following example, the present invention is described in further detail:

Example 1

Microelement pre-mix material of the present invention 40 parts, laying hen vitamin premix 8 parts, 70% lysine 10.8 parts, methionine 27 parts, phytase 3 parts, Santoquin 0.6 part, powdered rice hulls 10.6 parts mixing nucleation Heart material；

By core material 16.67 parts, Maifanitum 18 parts, 23.67 parts of microlith powder, calcium hydrogen phosphate 28 parts, little 2.67 parts of soda, Sal 8.3 parts, 50% choline chloride 2.67 parts are mixed into core premix；

By granular Fructus Anisi Stellati Semen Maydis 61.2 parts, bean cake (43%) 23.8 part, 3 parts of input pulverizers of DDGS Interior pulverizing, raw material pulverizing granularity is 4.0mm, puts into the powder stone powder 9 parts in Fructus Anisi Stellati, subsequently by 3 parts of cores Material premix material puts into mixer mixing, incorporation time 180s；Obtain the laying-hen compound feed of particle diameter about 4mm. Meanwhile, commercially common laying hen microelement pre-mix material produces laying hen complete feedstuff by same steps.

Mixed feed and the city of the microelement pre-mix material configuration improving eggshell strength of the present invention will be used The mixed feed of field common trace element configuration carries out contrast test.

Select blue brown laying hen 960 plumage in 380d health sea, be randomly divided into comparison I group, comparison II group and test group, Often 5 repetitions of group, each repetition 48 plumage, to implement lamination and raise in cages, breeding cycle is 35d, raises the phase the most in advance 7d.EXPERIMENTAL DESIGN such as table 2.During test, laying rate, egg size, feedstuff-egg ratio, eggshell strength, rate of broken eggs are entered Row statistics, such as table 3, table 4.

Table 2 EXPERIMENTAL DESIGN

Packet	Comparison I group	Comparison II group	Test group
				Daily ration	Common material 1	Common material 2	Testing material

Table 3 production performance

Packet	Laying rate/%	Average egg weight/g	Feedstuff-egg ratio
				Comparison I group	75.45±1.88	61.55±5.57	2.11±0.19b
Comparison II group	76.65±3.42	60.98±4.61	1.98±0.15a
				Test group	79.82±3.92	62.08±5.25	2.01±0.16a

Table 4 eggshell strength measured value classification

Packet	Eggshell strength/kgf	Percentage ratio/the % of >=3.0kgf	Percentage ratio/the % of >=4.0kgf	Rate of broken eggs/%
					Comparison I group	3.37±0.89	67.35	21.63	3.27
Comparison II group	3.46±0.87	69.83	27.69	1.24
					Test group	3.48±0.90	76.47	29.41	0.84

Note: require that egg can bear 2.65 × 105-3.5 × 105Pa (2.7-3.6kg/c m when placing vertically in the world²) pressure, according to surveying and determination, fowl egg exist Do not rupture under 2.94 × 105Pa (30 atmospheric pressure), therefore eggshell strength measured value is set to limit value more than 3.0, carries out hierarchical statistics.

The laying hen trace element of the present invention has certain facilitation to the raising of performance in layers, can be bright The aobvious eggshell strength improving fowl egg, improves comprcssive strength more than 3.0kgf/cm²、4.0kgf/cm²Egg institute Accounting example, thus substantially reduce egg rate of broken eggs in picking up egg, transportation.

Example 2:

Microelement pre-mix material of the present invention 40 parts, laying hen vitamin premix 8 parts, 70% lysine 10.8 parts, methionine 27 parts, phytase 3 parts, Santoquin 0.6 part, powdered rice hulls 10.6 parts mixing nucleation Heart material；

By core material 16.67 parts, Maifanitum 18 parts, 23.67 parts of microlith powder, calcium hydrogen phosphate 28 parts, little 2.67 parts of soda, Sal 8.3 parts, 50% choline chloride 2.67 parts are mixed into core premix；

By in granular Fructus Anisi Stellati Semen Maydis 62 parts, bean cake (43%) 22.5 part, 3 parts of input pulverizers of cottonseed meal Pulverizing, raw material pulverizing granularity is 4.0mm, puts into the powder stone powder 9 parts in Fructus Anisi Stellati, Adeps Sus domestica 0.5 part.Subsequently By 3 parts of input mixer mixing of core premix, incorporation time 180s；The laying hen obtaining particle diameter about 4mm is joined Close feedstuff.Meanwhile, the most common 0.2% laying hen microelement pre-mix material produces egg by same steps Chicken complete feedstuff.

Mixed feed and the city of the microelement pre-mix material configuration improving eggshell strength of the present invention will be used The mixed feed of field common trace element configuration carries out contrast test.

Select blue brown laying hen 480 plumage in 560d health sea, be randomly divided into comparison I group, comparison II group and test group, Often 4 repetitions of group, each repetition 30 plumage, to implement lamination and raise in cages, breeding cycle is 38d, raises the phase the most in advance 11d.EXPERIMENTAL DESIGN such as table 5.Laying rate, egg size, feedstuff-egg ratio, eggshell strength, rate of broken eggs are carried out by period Statistics, such as table 6, table 7.

Table 5 EXPERIMENTAL DESIGN

Packet	Comparison I group	Comparison II group	Comparison III group	Test group
					Daily ration	Common material 1	Common material 2	Common material 3	Testing material

Table 6 production performance

Packet	Laying rate/%	Average egg weight/g	Feedstuff-egg ratio
				Comparison I group	78.09±4.15	67.04±0.87	2.46±0.14
Comparison II group	79.32±6.89	66.63±0.82	2.35±0.05
				Comparison III group	77.35±4.75	66.27±1.37	2.48±0.11
Test group	81.14±4.33	66.31±0.68	2.30±0.15

Table 7 eggshell strength measured value classification

Packet	Eggshell strength/kgf	Percentage ratio/the % of >=3.0kgf	Percentage ratio/the % of >=4.0kgf	Rate of broken eggs/%
					Comparison I group	3.39±1.07	63.41	40.15	0.32±0.19
Comparison II group	3.34±0.90	66.67	37.93	0.46±0.29
					Comparison III group	3.11±1.01	55.84	37.21	0.54±0.22
Test group	3.40±0.89	68.60	46.68	0.28±0.19

Above-mentioned only several specific embodiments in the present invention are illustrated, but can not be as the guarantor of the present invention Protect scope, every according to the equivalence change done by the design spirit in the present invention or modification, all it is considered as Enter protection scope of the present invention.

Claims (2)

1. the microelement pre-mix material improving eggshell strength, it is characterised in that include following parts by weight Raw material: copper sulphate pentahydrate 1.5～2.5 parts, ferrous sulfate monohydrate 8～15 parts, manganese sulfate monohydrate 10～15 parts, Zinc sulfate monohydrate 5～8 parts, iodine 0.2～1 part, selenium 0.1～1 part, stone powder 35～40 parts, Maifanitum 30～40 parts With defatted rice bran 1～5 parts.

2. the preparation method of the microelement pre-mix material improving eggshell strength described in claim 1, its feature It is, comprises the following steps:

A. purification and impurity removal: remove the impurity in raw material described in claim 1, uses recrystallization method to remove Exceed standard in anhydrous cupric sulfate arsenic；

B. it is dried: each raw material is carried out 30 DEG C of dried 24h；

C. pulverize and sieve: each raw material is sieved, it is desirable to 100% passes through 35 mesh sieves, and 90%～95% passes through 100 mesh sieves；

D. micronized: iodine and selenium are mixed into trace element mixture；

E. agglomeration resistance processes: with calcium stearate or Bentonite, copper sulphate pentahydrate is carried out agglomeration resistance process；

F. dilution processes: increase the free-running property of trace element mixture with stone powder；

G. raw material mixing: be sufficiently mixed by all raw materials, prepares the microelement pre-mix material improving eggshell strength.