CN105838106A - Ultrahigh-pressure extraction method for pigment in fruit and vegetable - Google Patents
Ultrahigh-pressure extraction method for pigment in fruit and vegetable Download PDFInfo
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- CN105838106A CN105838106A CN201610203724.5A CN201610203724A CN105838106A CN 105838106 A CN105838106 A CN 105838106A CN 201610203724 A CN201610203724 A CN 201610203724A CN 105838106 A CN105838106 A CN 105838106A
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B61/00—Dyes of natural origin prepared from natural sources, e.g. vegetable sources
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B67/00—Influencing the physical, e.g. the dyeing or printing properties of dyestuffs without chemical reactions, e.g. by treating with solvents grinding or grinding assistants, coating of pigments or dyes; Process features in the making of dyestuff preparations; Dyestuff preparations of a special physical nature, e.g. tablets, films
- C09B67/0096—Purification; Precipitation; Filtration
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- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention relates to an ultrahigh-pressure extraction method for pigment in fruit and vegetable, belonging to the technical field of assisted extraction of natural pigment. The method comprises the following concrete steps: (1) cleaning and crushing fruit and vegetable (such as mulberry, blueberry and blackberry) rich in pigment, placing the fruit and vegetable and an extraction solvent in a high-pressure-resistant container and sealing the container; (2) subjecting the container filled with the fruit and vegetable to ultrahigh-pressure treatment at pressure of 300 to 600 Mpa for 5 to 20 min; and (3) after ultrahigh-pressure assisted extraction, carrying out filtering, collecting filtrate, and subjecting the filtrate to purifying, concentration and drying so as to obtain pigment. Compared with a traditional pigment extraction method, the method provided by the invention has the following advantages: since the extracted fruit and vegetable pigment does not undergo heat treatment, activity loss is small, the brilliant color of the pigment is better retained, extraction time is shortened, extraction efficiency is improved, and the method is widely applicable to the field of food processing.
Description
Technical field
The present invention relates to a kind of natural pigment auxiliary extraction technology field, belong to food processing field.More particularly to a kind of method extracting natural pigment from fruit and vegerable.
Background technology
Along with people's attention to food safety, the harm of synthetic food color increasingly comes into one's own, in fruit and vegerable, natural pigment not only has the highest safety and good coloring, also there is antioxidation, remove the biological activitys such as free radical, use natural pigment to substitute the development trend that synthetic food color becomes inevitable.
The extracting method of natural pigment is mainly solvent extraction method at present, but solvent extraction method also exists extraction time length, extraction ratio is low, need shortcomings such as repeatedly extracting, color valency is low；Carry out heating on the basis of solvent extraction method, ultrasonic Treatment, microwave treatment can improve pigment yield, shorten extraction time, but pigment declines along with temperature rises high stability, temperature the highest fall pigment solution is the fastest, and heating, ultrasound wave, microwave-assisted process and the degraded of pigment during extraction and biological activity all can be caused to reduce.
Summary of the invention
For the weak point of current solvent extraction method, the present invention provides a kind of pigment method in supertension assisted extraction fruit and vegerable, has that extraction time is short, extract yield high, stability and a biological activity advantages of higher.
In order to realize the purpose of foregoing invention, the present invention uses following technical scheme:
A kind of method of pigment in hyperpressure extraction fruit and vegerable, is carried out as steps described below:
1) fruit and vegerable rich in pigment such as Fructus Mori, blue berry, blackberry are chosen.
2) tissue mashing crusher machine is used after being cleaned by fruit and vegerable.
3) take the pulp after crushing to put in high-pressure-resistant vessel, and add Extraction solvent by certain solid-to-liquid ratio.
4) hyperpressure extraction is carried out.
5) extraction solution filtered, concentrate, be dried both product.
In step 3), described extractant is acid ethanol solution, water.Acidity alcohol solution concentration is 50%～80%(V/V), acid uses hydrochloric acid or citric acid, and in Extraction solvent, the whole content of acid is 0.5%～1.5%(V/V).
In step 3), described solid-to-liquid ratio is 1:20～1:50(g/mL).
In step 4), described ultra high pressure treatment pressure is 300Mpa～600Mpa, and the time is 5min～20min.
In step 5), described purification is macroporous resin column purification.
In step 5), described concentration technique uses vacuum-concentrcted or membrance concentration.
In step 5), described dry technology uses vacuum lyophilization or spray drying.
By technique scheme, the present invention has a following beneficial effect:
The present invention uses pigment in hyperpressure extraction fruit and vegerable, can shorten extraction time, improves and extract yield, reduction energy consumption compared with traditional solvent extraction method；Carry with hot dipping, ultrasonic assistant extracts and compared with microwave radiation exaraction, hyperpressure extraction process does not has hot-working, it is possible to reduce heating process to the degraded of pigment in fruit and vegerable, it is also possible to avoid heating destruction bioactive to pigment；Compared with biological enzyme, supercritical extraction, it is short that hyperpressure extraction has the cycle, low power consumption and other advantages.The present invention extracts gained pigment, and the cycle is short, less energy consumption, and biological activity retains complete, safe and reliable.
Detailed description of the invention
Embodiment 1
Take Fructus Mori 0.1kg, clean, dry after use tissue mashing crusher machine, will broken after pulp put in superelevation bag pressing, add 2L acid ethanol solution, wherein concentration of alcohol is 50%, acid is citric acid, and in Extraction solvent, citric acid content is 1%, and sealing machine seals, supertension 300MPa processes 40min, 1mL extracting solution Scavenging action to hydroxyl free radical reaches 86.4%, and pigment extraction ratio 58%, extracting solution i.e. obtains Fructus Mori pigment by vacuum-concentrcted and vacuum lyophilization after purification through macroporous resin column.The Fructus Mori pigment extraction time that the method prepares is short, and pigment biological activity is good, and extraction ratio is high.
Comparative example 1
Take Fructus Mori 0.1kg, clean, dry after use tissue mashing crusher machine, pulp after broken is added 2L acid ethanol solution, wherein concentration of alcohol is 50%, and acid is citric acid, and in Extraction solvent, citric acid content is 1%, 60 DEG C are extracted 1h, 1mL extracting solution Scavenging action to hydroxyl free radical is 68.3%, pigment extraction ratio 42%, and extracting solution i.e. obtains Fructus Mori pigment by vacuum-concentrcted and vacuum lyophilization after purification through macroporous resin column.
Embodiment 2
Take blue berry 0.1kg, clean, dry after use tissue mashing crusher machine, putting in high pressure resistant bottle by the pulp after broken, add 2.5L acid ethanol solution, wherein concentration of alcohol is 70%, acid is citric acid, in Extraction solvent, citric acid content is 1.5%, and it is 89.3% that supertension 500MPa processes 10min, 1mL extracting solution Scavenging action to hydroxyl free radical, pigment extraction ratio 61%, extracting solution i.e. obtains Blueberry pigment by micro-filtration membrane concentration and vacuum lyophilization after purification through macroporous resin column.The Blueberry pigment extraction time that the method prepares is short, and pigment biological activity is good, and extraction ratio is high.
Comparative example 2
Take blue berry 0.1kg, clean, dry after use tissue mashing crusher machine, pulp after broken is added 2.5L acid ethanol solution, wherein concentration of alcohol is 70%, and acid is citric acid, and in Extraction solvent, citric acid content is 1.5%, 30KHz ultrasonic extraction 30min, 1mL extracting solution Scavenging action to hydroxyl free radical is 71.3%, pigment extraction ratio 56%, and extracting solution i.e. obtains Blueberry pigment by micro-filtration membrane concentration and vacuum lyophilization after purification through macroporous resin column.
Embodiment 3
Take blackberry 0.1kg, clean, dry after use tissue mashing crusher machine, putting in superelevation bag pressing by the pulp after broken, add 5L acid water, acid is hydrochloric acid, in Extraction solvent, content of hydrochloric acid is 0.5%, sealing machine seals, and it is 85.9% that supertension 600MPa processes 5min, 1mL extracting solution Scavenging action to hydroxyl free radical, pigment extraction ratio 54.2%, extracting solution i.e. obtains blackberry pigment by micro-filtration membrane concentration with being spray-dried after purification through macroporous resin column.The Blueberry pigment extraction time that the method prepares is short, and pigment biological activity is good, and extraction ratio is high.
Comparative example 3
Take blackberry 0.1kg, clean, dry after use tissue mashing crusher machine, pulp after broken is added 5L acid water, acid is hydrochloric acid, in Extraction solvent, content of hydrochloric acid is 0.5%, and 60 DEG C are extracted 1h, 1mL extracting solution Scavenging action to hydroxyl free radical is 62.7%, pigment extraction ratio 43.5%, extracting solution i.e. obtains blackberry pigment by micro-filtration membrane concentration with being spray-dried after purification through macroporous resin column.
Claims (7)
1. the method for pigment in hyperpressure extraction fruit and vegerable, it is characterised in that carry out as steps described below:
(1) fruit and vegerable rich in pigment such as Fructus Mori, blue berry, blackberry are chosen；
(2) tissue mashing crusher machine is used after being cleaned by fruit and vegerable；
(3) take the pulp after crushing to put in high-pressure-resistant vessel, and add Extraction solvent by certain solid-to-liquid ratio；
(4) ultra high pressure treatment is carried out；
(5) by extraction solution through filtrations, purification, concentrate, be dried both obtained product.
The method of pigment in a kind of hyperpressure extraction fruit and vegerable the most according to claim 1, it is characterised in that the extractant described in step (3) is acid ethanol solution, water；Acidity alcohol solution concentration is 50%～80%(V/V), acid uses hydrochloric acid or citric acid, and in Extraction solvent, the whole content of acid is 0.5%～1.5%(V/V).
The method of pigment in a kind of hyperpressure extraction fruit and vegerable the most according to claim 1, it is characterised in that step (3) described solid-to-liquid ratio is 1:20～1:50(g/mL).
The method of pigment in a kind of hyperpressure extraction fruit and vegerable the most according to claim 1, it is characterised in that step (4) described ultra high pressure treatment pressure is 300Mpa～600Mpa, and the time is 5min～20min.
The method of pigment in a kind of hyperpressure extraction fruit and vegerable the most according to claim 1, it is characterised in that step (5) described purification uses macroporous resin column purification.
The method of pigment in a kind of hyperpressure extraction fruit and vegerable the most according to claim 1, it is characterised in that step (5) described concentration technique uses vacuum-concentrcted or membrance concentration.
7. according to the method for pigment in claim a kind of hyperpressure extraction fruit and vegerable, it is characterised in that step (5) described dry technology uses vacuum lyophilization or spray drying.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610203724.5A CN105838106A (en) | 2016-04-05 | 2016-04-05 | Ultrahigh-pressure extraction method for pigment in fruit and vegetable |
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| CN201610203724.5A CN105838106A (en) | 2016-04-05 | 2016-04-05 | Ultrahigh-pressure extraction method for pigment in fruit and vegetable |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106578825A (en) * | 2016-11-22 | 2017-04-26 | 河源农社食品有限公司 | Extraction method and application of passion fruit anthocyanin and passion fruit juice drink |
| CN106666313A (en) * | 2017-03-03 | 2017-05-17 | 佛山市三水区隐雪食品有限公司 | Blueberry drink and preparation method thereof |
| CN108967955A (en) * | 2018-08-01 | 2018-12-11 | 山东农业大学 | The extracting method of melanoidin in a kind of blacking jujube |
| CN112120232A (en) * | 2020-10-16 | 2020-12-25 | 宜宾西华大学研究院 | Method for continuously extracting pigment and dietary fiber from bamboo shoot shells |
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| CN101255280A (en) * | 2007-12-03 | 2008-09-03 | 江苏省中国科学院植物研究所 | Method for extracting blackberry pigment |
| CN103468020A (en) * | 2013-09-26 | 2013-12-25 | 江苏省中国科学院植物研究所 | Blueberry pigment extraction method |
| CN105062125A (en) * | 2015-07-29 | 2015-11-18 | 胡伟民 | Mulberry red pigment extraction and purification method |
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2016
- 2016-04-05 CN CN201610203724.5A patent/CN105838106A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101255280A (en) * | 2007-12-03 | 2008-09-03 | 江苏省中国科学院植物研究所 | Method for extracting blackberry pigment |
| CN103468020A (en) * | 2013-09-26 | 2013-12-25 | 江苏省中国科学院植物研究所 | Blueberry pigment extraction method |
| CN105062125A (en) * | 2015-07-29 | 2015-11-18 | 胡伟民 | Mulberry red pigment extraction and purification method |
Non-Patent Citations (2)
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| 杨瑞学: "超高压技术在食品加工领域的应用", 《农业工程》 * |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106578825A (en) * | 2016-11-22 | 2017-04-26 | 河源农社食品有限公司 | Extraction method and application of passion fruit anthocyanin and passion fruit juice drink |
| CN106666313A (en) * | 2017-03-03 | 2017-05-17 | 佛山市三水区隐雪食品有限公司 | Blueberry drink and preparation method thereof |
| CN108967955A (en) * | 2018-08-01 | 2018-12-11 | 山东农业大学 | The extracting method of melanoidin in a kind of blacking jujube |
| CN108967955B (en) * | 2018-08-01 | 2022-03-22 | 山东农业大学 | Method for extracting melanoidin from black red dates |
| CN112120232A (en) * | 2020-10-16 | 2020-12-25 | 宜宾西华大学研究院 | Method for continuously extracting pigment and dietary fiber from bamboo shoot shells |
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