CN105566507A - Preparation method of tea polysaccharides - Google Patents
Preparation method of tea polysaccharides Download PDFInfo
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- CN105566507A CN105566507A CN201510941916.1A CN201510941916A CN105566507A CN 105566507 A CN105566507 A CN 105566507A CN 201510941916 A CN201510941916 A CN 201510941916A CN 105566507 A CN105566507 A CN 105566507A
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- Organic Chemistry (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention provides a preparation method of tea polysaccharides, which comprises the following steps: drying, pulverizing, degreasing with a non-polar solvent, carrying out ultrasonic extraction, precipitating with an ethanol solution, purifying through an adsorption column or ultrafiltration membrane, and carrying out freeze-drying and the like. By using the crude old tea and aged tea as raw materials, the method solves the problem of waste of resources; the extraction and purification processes are simple and easy to implement; and the obtained tea polysaccharides have the advantages of high purity and no solvent residues.
Description
Technical field
The present invention relates to the processing technology of natural product, particularly a kind of preparation method of Tea Polysaccharides.
Background technology
China is one of main Tea Production state, and Tea planting area ranks first in the world, and tealeaves ultimate production is more than 1,000,000 tons.But only tender tea, newly picked and processed tea leaves are subject to the favor of people for a long time, and coarse tea leaf, old tea but can only overstock in warehouse or discard in tea place, cause the significant wastage of resource.
Research in recent years show Tea Polysaccharides have antitumor, anti-oxidant, antithrombotic, hypoglycemic hypotensive, increase the effect such as coronary artery blood flow and reducing blood-fat.In the experience also having normal drink coarse tea treatment diabetes among the people, and the Tea Polysaccharides content in coarse tea leaf is far above tender tealeaves.Therefore, from coarse tea leaf, old tea, extract active tea polysaccharide, making full use of of tea resources can be conducive to, increase economic efficiency, promote tealeaves development.
Summary of the invention
In view of this, the object of the invention is to, a kind of preparation method of Tea Polysaccharides is provided, especially a kind of method extracting Tea Polysaccharides from coarse tea leaf, old tea.
A preparation method for Tea Polysaccharides, comprises following steps:
S1: pulverize after tea air-drying or drying, utilizes pulverizer to be crushed to 50-90 order, obtains tea-leaf power;
S2: degreasing utilizes non-polar solvent to soak above-mentioned tea-leaf power, the solid-to-liquid ratio of non-polar solvent and tea-leaf power is 1:1-1:15, and soaking temperature is 20-40 DEG C, and soak time is 0.5-2h, and filter, filtration cakes torrefaction, obtains pretreated tea powder;
S3: extract the water that pretreated tea powder adds 10-70 quality multiple, 30-50KHz ultrasonic extraction, described Extracting temperature is 20-40 DEG C, and extraction time is 20-50min, and extraction time is 1-3 time, filters to obtain extracting solution after extraction;
S4: be deposited in extracting solution the aqueous ethanolic solution adding 45-90%, obtains supernatant liquor and throw out, and throw out is multiple water-soluble;
S5: purifying utilizes adsorption column or ultra-filtration membrane to carry out purifying to the above-mentioned sedimentary aqueous solution;
S6: lyophilize, by the Tea Polysaccharides aqueous solution lyophilize 10-15h after purifying, obtains Tea Polysaccharides.
Described tealeaves is old tea or coarse tea leaf.
Described tealeaves is the tea waste in production process in trimming leaf or Tea Processing process.
Described non-polar solvent is ether or sherwood oil.
Described water is deionized water.
Described adsorption column is polyamide column.
Described ultra-filtration membrane is the poly (ether sulfone) film of 50-200KD.
After tea air-drying, be more conducive to pulverizing, as tealeaves is too many, air-dry too time-consuming, the method for machine drying can be adopted.
Defatting step effectively can remove fat and some small molecular weight impurities of the encirclement of tealeaves extracellular lipid.
Supersonic extracting method is convenient and swift, does not need high temperature, can not destroy the oxidation activity of polysaccharide.
Adopt alcohol settling, Tea Polysaccharides purity prepared after adsorption column or ultra-filtration membrane purifying is higher, no solvent residue.
Beneficial effect of the present invention is that solve the problem of the wasting of resources, extracting and purifying method is simple simultaneously, and gained Tea Polysaccharides purity is high, no solvent residue with coarse tea leaf, old tea for raw material.
Embodiment
A preparation method for Tea Polysaccharides, comprises following steps:
S1: pulverize after tea air-drying or drying, utilizes pulverizer to be crushed to 50-90 order, obtains tea-leaf power;
S2: degreasing utilizes non-polar solvent to soak above-mentioned tea-leaf power, the solid-to-liquid ratio of non-polar solvent and tea-leaf power is 1:1-1:15, and soaking temperature is 20-40 DEG C, and soak time is 0.5-2h, and filter, filtration cakes torrefaction, obtains pretreated tea powder;
S3: extract the water that pretreated tea powder adds 10-70 quality multiple, 30-50KHz ultrasonic extraction, described Extracting temperature is 20-40 DEG C, and extraction time is 20-50min, and extraction time is 1-3 time, filters to obtain extracting solution after extraction;
S4: be deposited in extracting solution the aqueous ethanolic solution adding 45-90%, obtains supernatant liquor and throw out, and throw out is multiple water-soluble;
S5: purifying utilizes adsorption column or ultra-filtration membrane to carry out purifying to the above-mentioned sedimentary aqueous solution;
S6: lyophilize, by the Tea Polysaccharides aqueous solution lyophilize 10-15h after purifying, obtains Tea Polysaccharides.
Described tealeaves is old tea or coarse tea leaf.
Described tealeaves is the tea waste in production process in trimming leaf or Tea Processing process.
Described non-polar solvent is ether or sherwood oil.
Described water is deionized water.
Described adsorption column is polyamide column.
Described ultra-filtration membrane is the poly (ether sulfone) film of 50-200KD.
Below by specific embodiment, the present invention is described further.
Embodiment 1
After air-dry for coarse tea leaf, utilize pulverizer to be crushed to 50 orders, obtain tea-leaf power; Utilize ether to soak above-mentioned tea-leaf power, the solid-to-liquid ratio of ether and tea-leaf power is 1:15, and soaking temperature is 40 DEG C, and soak time is 0.5h, and filter, filtration cakes torrefaction, obtains pretreated tea powder; Pretreated tea powder adds the water of 70 quality multiples, 50KHz ultrasonic extraction, and described Extracting temperature is 20 DEG C, and extraction time is 50min, and extraction time is 1 time, filters to obtain extracting solution after extraction; In extracting solution, add the aqueous ethanolic solution of 45%, obtain supernatant liquor and throw out, throw out is multiple water-soluble; Adsorption column or ultra-filtration membrane is utilized to carry out purifying to the above-mentioned sedimentary aqueous solution; By the Tea Polysaccharides aqueous solution lyophilize 15h after purifying, obtain Tea Polysaccharides.
Embodiment 2
After air-dry for coarse tea leaf, utilize pulverizer to be crushed to 90 orders, obtain tea-leaf power; Utilize ether to soak above-mentioned tea-leaf power, the solid-to-liquid ratio of ether and tea-leaf power is 1:1, and soaking temperature is 40 DEG C, and soak time is 2h, and filter, filtration cakes torrefaction, obtains pretreated tea powder; Pretreated tea powder adds the water of 10 quality multiples, 40KHz ultrasonic extraction, and described Extracting temperature is 40 DEG C, and extraction time is 20min, and extraction time is 3 times, filters to obtain extracting solution after extraction; In extracting solution, add the aqueous ethanolic solution of 90%, obtain supernatant liquor and throw out, throw out is multiple water-soluble; Adsorption column or ultra-filtration membrane is utilized to carry out purifying to the above-mentioned sedimentary aqueous solution; By the Tea Polysaccharides aqueous solution lyophilize 10h after purifying, obtain Tea Polysaccharides.
Embodiment 3
After air-dry for old tea, utilize pulverizer to be crushed to 70 orders, obtain tea-leaf power; Utilize sherwood oil to soak above-mentioned tea-leaf power, the solid-to-liquid ratio of sherwood oil and tea-leaf power is 1:10, and soaking temperature is 30 DEG C, and soak time is 1h, and filter, filtration cakes torrefaction, obtains pretreated tea powder; Pretreated tea powder adds the water of 40 quality multiples, 30KHz ultrasonic extraction, and described Extracting temperature is 30 DEG C, and extraction time is 30min, and extraction time is 2 times, filters to obtain extracting solution after extraction; In extracting solution, add the aqueous ethanolic solution of 60%, obtain supernatant liquor and throw out, throw out is multiple water-soluble; Adsorption column or ultra-filtration membrane is utilized to carry out purifying to the above-mentioned sedimentary aqueous solution; By the Tea Polysaccharides aqueous solution lyophilize 12h after purifying, obtain Tea Polysaccharides.
Embodiment 4
After leftover bits and pieces tea air-drying, utilize pulverizer to be crushed to 80 orders, obtain tea-leaf power; Utilize ether to soak above-mentioned tea-leaf power, the solid-to-liquid ratio of ether and tea-leaf power is 1:5, and soaking temperature is 30 DEG C, and soak time is 1.5h, and filter, filtration cakes torrefaction, obtains pretreated tea powder; Pretreated tea powder adds the water of 50 quality multiples, 40KHz ultrasonic extraction, and described Extracting temperature is 30 DEG C, and extraction time is 40min, and extraction time is 2 times, filters to obtain extracting solution after extraction; In extracting solution, add the aqueous ethanolic solution of 80%, obtain supernatant liquor and throw out, throw out is multiple water-soluble; Adsorption column or ultra-filtration membrane is utilized to carry out purifying to the above-mentioned sedimentary aqueous solution; By the Tea Polysaccharides aqueous solution lyophilize 14h after purifying, obtain Tea Polysaccharides.
The foregoing is only embodiments of the present invention; not thereby the scope of the claims of the present invention is limited; every utilize description of the present invention to do equivalent structure or equivalent flow process conversion; or be directly or indirectly used in other relevant technical fields, be all in like manner included in scope of patent protection of the present invention.
Claims (7)
1. a preparation method for Tea Polysaccharides, is characterized in that comprising following steps:
S1: pulverize after tea air-drying or drying, utilizes pulverizer to be crushed to 50-90 order, obtains tea-leaf power;
S2: degreasing utilizes non-polar solvent to soak above-mentioned tea-leaf power, the solid-to-liquid ratio of non-polar solvent and tea-leaf power is 1:1-1:15, and soaking temperature is 20-40 DEG C, and soak time is 0.5-2h, and filter, filtration cakes torrefaction, obtains pretreated tea powder;
S3: extract the water that pretreated tea powder adds 10-70 quality multiple, 30-50KHz ultrasonic extraction, described Extracting temperature is 20-40 DEG C, and extraction time is 20-50min, and extraction time is 1-3 time, filters to obtain extracting solution after extraction;
S4: be deposited in extracting solution the aqueous ethanolic solution adding 45-90%, obtains supernatant liquor and throw out, and throw out is multiple water-soluble;
S5: purifying utilizes adsorption column or ultra-filtration membrane to carry out purifying to the above-mentioned sedimentary aqueous solution;
S6: lyophilize, by the Tea Polysaccharides aqueous solution lyophilize 10-15h after purifying, obtains Tea Polysaccharides.
2. the preparation method of Tea Polysaccharides as claimed in claim 1, is characterized in that described tealeaves is old tea or coarse tea leaf.
3. the preparation method of Tea Polysaccharides as claimed in claim 1, is characterized in that described tealeaves is the tea waste in production process in trimming leaf or Tea Processing process.
4. the preparation method of Tea Polysaccharides as claimed in claim 1, is characterized in that described non-polar solvent is ether or sherwood oil.
5. the preparation method of Tea Polysaccharides as claimed in claim 1, is characterized in that described water is deionized water.
6. the preparation method of Tea Polysaccharides as claimed in claim 1, is characterized in that described adsorption column is polyamide column.
7. the preparation method of Tea Polysaccharides as claimed in claim 1, is characterized in that described ultra-filtration membrane is the poly (ether sulfone) film of 50-200KD.
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| CN201510941916.1A CN105566507A (en) | 2015-12-16 | 2015-12-16 | Preparation method of tea polysaccharides |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106995498A (en) * | 2017-06-07 | 2017-08-01 | 安徽中盛食用油科技有限公司 | Excellent tea seed polysaccharide based surfactants of a kind of emulsifiability and preparation method thereof |
| CN107936133A (en) * | 2018-01-16 | 2018-04-20 | 吉林大学 | A kind of redsepal evening primrose leaf polysaccharide and preparation method thereof |
| CN108077490A (en) * | 2017-12-06 | 2018-05-29 | 芜湖市欣然食品有限公司 | A kind of preparation method of anti-ageing healthcare Ilex Latifolia Thunb polyoses grain |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102336841A (en) * | 2011-10-18 | 2012-02-01 | 中国计量学院 | Method for extracting tea polysaccharide |
| CN104177508A (en) * | 2014-08-06 | 2014-12-03 | 南昌大学 | Method for comprehensively extracting tea seed saponin, tea seed polypeptide and tea seed polysaccharide from tea seed cake |
| KR20140141883A (en) * | 2013-05-31 | 2014-12-11 | 다당앤(주) | Supercritical fluid technology Method for preparing polysaccharide of green tea |
| CN104383123A (en) * | 2014-11-14 | 2015-03-04 | 程金生 | Method for preparing golden camellia lipid-lowering blood sugar-reducing preparation |
| CN105061622A (en) * | 2015-08-07 | 2015-11-18 | 广西南宁派腾科技有限公司 | Extraction process of tea polysaccharide |
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2015
- 2015-12-16 CN CN201510941916.1A patent/CN105566507A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102336841A (en) * | 2011-10-18 | 2012-02-01 | 中国计量学院 | Method for extracting tea polysaccharide |
| KR20140141883A (en) * | 2013-05-31 | 2014-12-11 | 다당앤(주) | Supercritical fluid technology Method for preparing polysaccharide of green tea |
| CN104177508A (en) * | 2014-08-06 | 2014-12-03 | 南昌大学 | Method for comprehensively extracting tea seed saponin, tea seed polypeptide and tea seed polysaccharide from tea seed cake |
| CN104383123A (en) * | 2014-11-14 | 2015-03-04 | 程金生 | Method for preparing golden camellia lipid-lowering blood sugar-reducing preparation |
| CN105061622A (en) * | 2015-08-07 | 2015-11-18 | 广西南宁派腾科技有限公司 | Extraction process of tea polysaccharide |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106995498A (en) * | 2017-06-07 | 2017-08-01 | 安徽中盛食用油科技有限公司 | Excellent tea seed polysaccharide based surfactants of a kind of emulsifiability and preparation method thereof |
| CN108077490A (en) * | 2017-12-06 | 2018-05-29 | 芜湖市欣然食品有限公司 | A kind of preparation method of anti-ageing healthcare Ilex Latifolia Thunb polyoses grain |
| CN107936133A (en) * | 2018-01-16 | 2018-04-20 | 吉林大学 | A kind of redsepal evening primrose leaf polysaccharide and preparation method thereof |
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Application publication date: 20160511 |