CN106578825A - Extraction method and application of passion fruit anthocyanin and passion fruit juice drink - Google Patents
Extraction method and application of passion fruit anthocyanin and passion fruit juice drink Download PDFInfo
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- CN106578825A CN106578825A CN201611050080.7A CN201611050080A CN106578825A CN 106578825 A CN106578825 A CN 106578825A CN 201611050080 A CN201611050080 A CN 201611050080A CN 106578825 A CN106578825 A CN 106578825A
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- passion fruit
- anthocyanin
- fruit juice
- passion
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- 238000000605 extraction Methods 0.000 title claims abstract description 23
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/02—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation containing fruit or vegetable juices
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/02—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation containing fruit or vegetable juices
- A23L2/04—Extraction of juices
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses an extraction method of passion fruit anthocyanin. The extraction method comprises the steps of 1, choosing passion fruit and peel, and drying; 2, screening; 3, smashing; 4, conducting high-pressure extraction on the mixture of the smashed passion fruit and the peel; 5, filtrating; 6, conducting vacuum concentration and drying to obtain passion fruit anthocyanin. According to the extraction method of passion fruit anthocyanin, electric power is used, heat sources of a boiler and the like are not needed, and no waste matter and waste gases of dust, slag and the like are discharged; the solvent is recyclable in the extraction process, and no waste liquor is discharged. The extracted waste residues can also be used as a bioorganic fertilizer, and the extraction method is a fully green manufacturing technology. When passion fruit anthocyanin extracted from the method is applied in passion fruit juice drink, the passion fruit juice drink can be more natural in color, nutritional components can be more fully preserved, and the health care function of the product can be improved; moreover, the passion fruit juice drink has better antioxidation, and shelf life can be prolonged.
Description
Technical field
The present invention relates to natural materials are extracted and food soft drink industry, and in particular to the extraction side of passion fruit anthocyanin
Method, application and passion fruit juice drinks.
Background technology
Passion fruit also known as passion fruit, Brazil nut, China main producing region is the provinces such as Guangdong, Guangxi, Taiwan, Fujian.Passion fruit
Fruit juice nutrient material abundance, aromatic flavour is unique, is described as " fruit monosodium glutamate ", " king of fruit juice ", and research finds to contain in passion fruit
Some chemical composition abundant species, mainly including anthocyanin class, fragrance ingredient, cyanogenic glycoside, alkaloids, triterpenes, grease,
Carotenoid, mineral element, amino acid and polysaccharide etc., with anti-oxidant, promoting blood circulation are kept fit, enriching yin and nourishing kidney, refresh oneself and sober up, change
The health values such as kind diabetes.
With the improvement of people's living standards, the requirement to beverage is also improved constantly, and the food materials of green, ecosystem are increasingly
Praised highly, market potential is huge.Passion fruit is nutritious, sweet and sour taste, meets healthy trend, and making beverage has very big assurance
Get a share beverage market.But to select the of a relatively high raw material of acidity in due to processing in soft drink, and such passion fruit fruit juice
Color is not necessarily optimal, inevitably has to raw material in fruit juice production necessarily destroy in addition, and the impact to color and luster is especially
Seriously.
In addition, at present passion fruit mainly passes through decoction, backflow, ultrasonic wave, microwave, supercritical CO2Extraction etc. method
Anthocyanin is extracted, and these method high temperatures easily cause the denaturation of active ingredient, ultrasonic wave, microwave extract method are difficult to realize
Commercial scale, and supercritical CO2Only it is suitable to extract liposoluble substance.Therefore using a kind of efficient extracting method to passion fruit
Comprehensive development and utilization is significant.
The content of the invention
It is an object of the invention to overcome shortcoming and defect of the prior art, there is provided a kind of extraction of passion fruit anthocyanin
Method, using electric energy, it is not necessary to waste, the waste gas discharge such as the thermal source such as boiler, free from dust, slag；Solvent can in extraction process
To reclaim, also without discharging of waste liquid.Extraction waste residue is also used as bio-organic fertilizer and uses, and is a kind of completely green manufacturing
Technology.
The present invention is achieved by the following technical solutions：
The extracting method of passion fruit anthocyanin, it is characterised in that：Step is as follows：
(1) passion fruit and pericarp, drying are chosen；
(2) screen, remove the passion fruit and pericarp of debris and brown stain；
(3) crush；
(4) passion fruit and pericarp mixture of crushing are carried out into normal temperature high voltage extraction：Extractant is ethanol solution, passion fruit
It is 1 with the weight ratio of pericarp mixture and extractant：20, pressure 150Mpa is set, the time is 20～60min, and suction filtration retains filter
Liquid；
(5) filter, obtain passion fruit anthocyanin leaching liquor；
(6) drying concentrated in vacuo, obtains passion fruit anthocyanin.
Preferably, the drying condition of step (1) is dried 12 hours at 40 DEG C.
Preferably, 20 mesh sieves are crossed after step (3) is crushed.
Preferably, step (4) extractant is 80% ethanol.
Preferably, the drying concentrated in vacuo of step (6) is specifically：Passion fruit anthocyanin leaching liquor is positioned over into traditional vacuum
Concentrating instrument is spray-dried concentrate, 130 DEG C of EAT to ethanol volatilization completely, and 80 DEG C of leaving air temp enters material temperature
Spend for 50 DEG C.
The present invention also provides application of the passion fruit anthocyanin for preparing as stated above in passion fruit juice drinks.
The present invention also provides a kind of preparation method of passion fruit juice drinks, it is characterised in that：Step is as follows：
(1) choosing fruit：Select unabroken new foresythia passion fruit；
(2) squeeze the juice：The pulp of passion fruit is dug out and is put into rapidly in mixer, is stirred at 30 DEG C of temperature control；
(3) digest：The pectase of 0.25% pulp weight is added, at 45 DEG C 2.5h is digested, obtain Passion Fruit Juice；
(4) colloid mill grinding：Passion Fruit Juice is ground by colloid mills twice；
(5) reconcile：After the passion fruit anthocyanin prepared using said method is dissolved in water, and stabilizer, honey and Vc
Pour crutcher mediation into together；
(6) homogeneous：Homogeneous 1min under the conditions of 50MPa, homogeneous is once；
(7) ultra high temperature short time sterilization：5～8 seconds plus thermal sterilization are kept at 130 DEG C -150 DEG C, is then cooled down rapidly；
(8) it is canned：Fill empty bottle, stand after 1min immediately with being originally water-cooled to normal temperature.
Further, in step (5), relative to passion fruit juice drinks gross weight, the weight percentage for being added water is
65%, the weight percentage of stabilizer is 1.22%, and the weight percentage of honey is the weight percentage of 2.4%, Vc
For 1%.
Further, speed of agitator is 70 revs/min in step (2).
The present invention also provides a kind of passion fruit juice drinks, it is characterised in that：Using above-mentioned passion fruit fruit sweat drink system
Preparation Method is obtained.Passiflora edulis fruit juice beverage low sugar low-heat, special taste, green, the natural, health, and it is natural by adding
Passion fruit anthocyanin, give its unique health-care efficacy.
The extracting method of the passion fruit anthocyanin of the present invention, extract active ingredient of natural product used time is short, recovery rate is high；
Using electric energy, it is not necessary to waste, the waste gas discharge such as the thermal source such as boiler, free from dust, slag；Solvent can be returned in extraction process
Receive, so also without discharging of waste liquid.Extraction waste residue is also used as bio-organic fertilizer and uses, and is a kind of completely green manufacturing
Technology.Compared with prior art, the passion fruit anthocyanin that the present invention is prepared has higher Scavenging ability, and this is
Because normal temperature high voltage is at normal temperatures using high pressure technique by intracellular chemistry composition dissolution, the original activity of extract is maintained,
The active ingredient avoided in extraction process wrecks because of high temperature, thus irreplaceable excellent with other extractive techniques
Gesture.
, due to the addition of passion fruit anthocyanin, passion fruit juice color is more natural, more for the passion fruit juice drinks of the present invention
Fully save the nutrient content of passion fruit from damage, improve the health care of product；And the passion fruit juice drinks of the present invention have very
Good antioxidation, can Shelf-life.
Description of the drawings
Fig. 1 is the extracting method schematic diagram of the passion fruit anthocyanin of the present invention；
Fig. 2 is the preparation method schematic diagram of the passion fruit juice drinks of the present invention；
Fig. 3 is the calibration curve of Cy-3-G；
Fig. 4 is passion fruit Anthocyanin-rich Extract to DPPH free radical scavenging activities.
Specific embodiment
The preferred embodiments of the present invention are illustrated below in conjunction with accompanying drawing, it will be appreciated that preferred reality described herein
Apply example and be merely to illustrate and explain the present invention, be not intended to limit the present invention.
Embodiment 1
Such as Fig. 1, the extracting method of the passion fruit anthocyanin of the present embodiment, step is as follows：
(1) passion fruit and pericarp, drying are chosen；
(2) screen, remove the passion fruit and pericarp of debris and brown stain；
(3) crush；
(4) passion fruit and pericarp mixture of crushing are carried out into normal temperature high voltage extraction：Extractant be 80% ethanol solution, hundred
Fragrant fruit and pericarp mixture and the weight ratio of extractant are 1：20, pressure 150Mpa is set, the time is 20min, and suction filtration retains filter
Liquid；
(5) filter, obtain passion fruit anthocyanin leaching liquor；
(6) drying concentrated in vacuo, obtains passion fruit anthocyanin.
Embodiment 2
Such as Fig. 1, the extracting method of the passion fruit anthocyanin of the present embodiment, step is as follows：
(1) passion fruit and pericarp, drying are chosen：Dry 12 hours at 40 DEG C；
(2) screen, remove the passion fruit and pericarp of debris and brown stain；
(3) crush, cross 20 mesh sieves；
(4) passion fruit and pericarp mixture of crushing are carried out into normal temperature high voltage extraction：Extractant be 80% ethanol solution, hundred
Fragrant fruit and pericarp mixture and the weight ratio of extractant are 1：20, pressure 150Mpa is set, the time is 60min, and suction filtration retains filter
Liquid；
(5) filter, obtain passion fruit anthocyanin leaching liquor；
(6) drying concentrated in vacuo：Passion fruit anthocyanin leaching liquor is positioned over into traditional vacuum concentrating instrument to be evaporated completely to ethanol
Quan Hou, concentrate is spray-dried, 130 DEG C of EAT, 80 DEG C of leaving air temp, and feeding temperature is 50 DEG C, obtains passion fruit
Anthocyanin.
Embodiment 3
Such as Fig. 2, the preparation method of the passion fruit juice drinks of the present embodiment, step is as follows：
(1) choosing fruit：Select unabroken new foresythia passion fruit；
(2) squeeze the juice：The pulp of passion fruit is dug out and is put into rapidly in mixer, is stirred at 30 DEG C of temperature control, rotating speed is 70
Rev/min；
(3) digest：The pectase of 0.25% pulp weight is added, at 45 DEG C 2.5h is digested, obtain Passion Fruit Juice；
(4) colloid mill grinding：Passion Fruit Juice is ground by colloid mills twice；
(5) reconcile：After the passion fruit anthocyanin prepared by the method for embodiment 1 is dissolved in water, and stabilizer, honey and Vc
Pour crutcher mediation into together, relative to passion fruit juice drinks gross weight, the weight percentage for being added water is 65%, stabilizer
Weight percentage be 1.22%, the weight percentage of honey is 1% for the weight percentage of 2.4%, Vc；
(6) homogeneous：Homogeneous 1min under the conditions of 50MPa, homogeneous is once；
(7) ultra high temperature short time sterilization：8 seconds plus thermal sterilization are kept at 130 DEG C, is then cooled down rapidly；
(8) it is canned：Fill empty bottle, stand after 1min immediately with being originally water-cooled to normal temperature.
Embodiment 4
Such as Fig. 2, the preparation method of the passion fruit juice drinks of the present embodiment, step is as follows：
(1) choosing fruit：Select unabroken new foresythia passion fruit；
(2) squeeze the juice：The pulp of passion fruit is dug out and is put into rapidly in mixer, is stirred at 30 DEG C of temperature control, rotating speed is 70
Rev/min；
(3) digest：The pectase of 0.25% pulp weight is added, at 45 DEG C 2.5h is digested, obtain Passion Fruit Juice；
(4) colloid mill grinding：Passion Fruit Juice is ground by colloid mills twice；
(5) reconcile：After the passion fruit anthocyanin prepared by the method for embodiment 2 is dissolved in water, and stabilizer, honey and Vc
Pour crutcher mediation into together, relative to passion fruit juice drinks gross weight, the weight percentage for being added water is 65%, stabilizer
Weight percentage be 1.22%, the weight percentage of honey is 1% for the weight percentage of 2.4%, Vc, wherein surely
Agent is determined including xanthans 0.02% and carboxymethylcellulose calcium 1.2%；
(6) homogeneous：Homogeneous 1min under the conditions of 50MPa, homogeneous is once；
(7) ultra high temperature short time sterilization：5 seconds plus thermal sterilization are kept at 150 DEG C, is then cooled down rapidly；
(8) it is canned：Fill empty bottle, stand after 1min immediately with being originally water-cooled to normal temperature.
The comparison of passion fruit anthocyanin recovery rate under 5 three kinds of extracting methods of embodiment
Normal temperature high voltage of the present invention is respectively adopted to extract and normal reflux, three kinds of methods of ultrasonic extraction, passion fruit is extracted effective
Composition, the recovery rate with passion fruit anthocyanin carries out quantitative comparison as index, and its experimental result is following (table 1)：
The comparison of 1. 3 kinds of extracting method recovery rates of table
Result above shows：Used time of high pressure extraction active ingredient of natural product is most short, recovery rate highest.This is because molten
Agent quickly enters cell interior under very high pressure differential effect by osmosis, and cell there occurs and once destroy；Meanwhile,
Under condition of high voltage effect, active ingredient dissolves rapidly；In the case where concentration difference is promoted, active ingredient is by infiltration by intracellular release
Out；Piston is twitched back and forth, and pressurization release promotes extract circulation multiple, drives cycles samples, is more beneficial for active ingredient
Infiltration.
The identification of the passion fruit anthocyanin of embodiment 6 and antioxidation activity are analyzed
(1) instrument and material agents：
Instrument：The highly effective liquid phase chromatographic system Agilent company of the U.S. of Agilent 1260
ME215S assay balances Sartorius AG
Shimadzu Corporation of UV-2600 ultraviolet-uisible spectrophotometers Japan
EYELA Rotary Evaporators Shanghai Ai Lang Instrument Ltds
SHZ-DA circulating water type vavuum pump Shanghai and too equipment Co., Ltd
Reagent：
Passion fruit：Liuzhou City of Guangdong Province fruit wholesale market was purchased from 2016, passion fruit, pericarp are placed in 30 DEG C of baking oven and do
Dry 20h, crushed 60 mesh sieves, was positioned over 4 DEG C of freezen protectives.
Cy-3-G HPLC purity is more than 98% Chengdu Man Site biotechnologies company
TCI Reagent Companies of 1,1- diphenyl -2- trinitrophenyl-hydrazines (DPPH) Japan
TCI Reagent Companies of 2,6 di tert butyl 4 methyl phenol (BHT) Japan
Absolute ethyl alcohol Chinasun Specialty Products Co., Ltd
Methyl alcohol chromatogram rank Anhui Shi Lian special reagents limited company
(2) preparation of passion fruit Anthocyanin-rich Extract
The each 0.800g of passion fruit anthocyanin powder that three kinds of method extractions in above-mentioned table 1 are obtained, deionized water constant volume
To 80mL.The AB-8 macroreticular resins wet method of 100g activation process is loaded into glass exchange column, sample is fully adsorbed.With 7BV's
1%HCl rinses pillar to remove the impurity such as sugar, protein, afterwards with the parsing sample of 70% ethanol containing 1%HCl until efflux
Without color.It is concentrated by evaporation at 35 DEG C and removes solvent, with absolute ethyl alcohol fume hood is transferred to, fully grinds after solvent volatile dry
Grind passion fruit Anthocyanin-rich Extract is followed successively by：0.554g、0.654g、0.721g.
(3) HPLC methods determine the content of anthocyanin composition in passion fruit Anthocyanin-rich Extract
With Cy-3-G as standard items, the concentration with Cy-3-G as abscissa, with
Peak area is ordinate, draws the calibration curve of Cy-3-G, brings the peak area of each anthocyanin composition into arrow
The calibration curve of Che Jusu -3- glucosides calculates each anthocyanin content.Respectively precision weighs the above-mentioned three kinds of passion fruits of 1.04mg
Anthocyanin-rich Extract is dissolved and constant volume in 2mL volumetric flasks with 10% formic acid, diluted HPLC point is after miillpore filter successively
Analysis.
HPLC analyzes chromatographic condition：Chromatographic column is Cossmosil 5C18-AR- II (4.6mm × 250mm, 4 μm)；Flowing
Phase A be methyl alcohol, Mobile phase B be 10% formic acid, gradient：0-5min 10%A, 5-20min 10%-60%A, 20-25min
60%A；Flow velocity 1mL/min；20 DEG C of column temperature；Detection wavelength 520nm；The μ L of sample size 10.
(4) experimental result：The content of anthocyanin component in extract
Cy-3-G is linear good in the concentration range of 1-125 μ g/mL, and linear equation is Y=
38.644X+5.8451(R2=0.9996, n=5).Wherein Y is peak area (m AU), and X is that Cy-3-G is dense
Degree (μ g/mL), calibration curve is as shown in Figure 3.
Using Cy-3-G as quantitative criterion, therefore each anthocyanin composition in passion fruit Anthocyanin-rich Extract
Content it is as shown in table 2.
Table 2：Each anthocyanin and Anthocyanin content in passion fruit Anthocyanin-rich Extract
B is represented [(anthocyanin weight/extract weight) × 100%]
Anthocyanin content reaches 16.383% in the polyphenol substance that as known from Table 2 normal temperature high voltage is extracted in extract, no
By being qualitative or quantitative, refluxing extraction and ultrasonic extraction are above.This is because normal temperature high voltage adopts at normal temperatures high pressure skill
Intracellular chemistry composition dissolution maintains the original activity of extract by art, it is to avoid the active ingredient in extraction process is because of height
Temperature and wreck, thus with the irreplaceable advantage of other extractive techniques.
(5) anthocyanin is tested to DPPH free radical scavenging activities
Add the solution and 2.5mL DPPH ethanol solutions of 0.5mL variable concentrations extracts toward test tube successively
(0.2mmol/l) it is sufficiently mixed, avoid light place 30min surveys its absorbance A after 517nm under room temperature；Same operation, is gone with equivalent
Ionized water replaces the solution of extract, surveys its absorbance A 0；Replace DPPH solution with equivalent absolute ethyl alcohol, be separately added into 0.5mL
The solution of variable concentrations extract, is B with its absorbance is surveyed after method operation.Using BHT as positive control.Operation repetitive three times,
DPPH free radical scavenging activities according to the following formula：
DPPH free radical scavenging activities (%)=[1- (A-B)/A0] × 100%
As shown in Figure 4：In 0-1000 μ g/mL concentration ranges, the DPPH radicals scavengings of extract take the lead in concentration
Increase and increase, when concentration be more than 800 μ g/mL when clearance rate gently increase.Extracted by calculating passion fruit anthocyanin
Thing is 303.723 ± 3.182 μ g/mL to the median elimination concentration (IC50) of DPPH free radicals, and its IC50 is less than BHT (747.916
± 2.374 μ g/mL) but more than Vc (55.488 ± 2.082 μ g/mL).More little then its removing DPPH free radical scavenging activity of IC50
It is stronger.Therefore the DPPH free radical scavenging activities of passion fruit Anthocyanin-rich Extract are better than BHT but not as good as Vc.
The target level of product quality of embodiment 7
The passion fruit juice drinks prepared according to embodiment 3, subjective appreciation is as follows：
Color and luster：It is faint yellow；
Mouthfeel:Taste is soft, refrigerant tasty and refreshing, moderately sour and sweet；
Fragrance：With the distinctive strong nature of passion fruit；
Tissue morphology：Uniformly, without lamination, long-time is placed has allowed a small amount of pulp to precipitate.
Microbiological indicator
Total number of bacteria≤100/mL；Coliform bacterium≤3/mL；Pathogenic bacteria must not detect
Physico-chemical tests
(1) measure of reduced sugar：Direct titrimetric method
Principle：In a heated condition, indicator is made from methylene blue, sample liquid titration Jing reduced sugar standard liquids are calibrated
Basic copper tartrate solution, finally according to sample liquid consumption calculating the content of reduced sugar.
Demarcate copper tartrate solution：Take 5.0mL tartaric acid second liquid to be placed in 150mL conical flasks with 5.0mL tartaric acid solution As,
And 10mL distilled water is added, while about 9mL glucose is added dropwise enters conical flask.Firepower size is regulated, boils mixed liquor in 2min
Rise, keep being titrated with the speed of two seconds one drops while boiling, blueness is just taken off in solution, while recording final sample liquid
Scale.Operation repetitive 3 times, is defined by mean consumption volume.
It is calculated as follows：A=C × V
A- copper tartrate solutions (the first and second mixed liquors) are equivalent to glucose quality, unit mg；
C-glucose standard concentration, unit is mg/mL；
Consumption of glucose titer cumulative volume during V-demarcation, unit is mL.
Sample is titrated：Take 5.0mL tartaric acid second liquid to be placed in 150mL conical flasks with 5.0mL tartaric acid solution As, and add
10mL distilled water, while the sample that 1mL fewer than pre- titration volumes is added dropwise enters conical flask.Firepower size is regulated, makes to mix in 2min
Liquid boiling is closed, the speed titration dripped with two seconds a while keeping boiling, blueness is just taken off in solution, while record is most
Whole sample liquid scale.Operation repetitive 3 times, is defined by mean consumption volume.
Computational methods：Content of reducing sugar (with glucose meter) is calculated as follows：
X-sample reduction sugared content (with glucose meter), unit is g/100g；
Equivalent to glucose quality, unit is mg to A-copper tartrate solution (the first and second mixed liquors)；
M-sample mass, unit is g；
V-mean consumption volume of sample, unit is mL.
(2) measure of ascorbic acid (Vc)：2,6-dichloroindophenol titration
Principle：2,6- dichloroindophenol solution show in acid condition pink (showing blueness under alkalescence or neutrallty condition), quilt
Color disappears after reduction type ascorbic acid reduction.In the presence of free from admixture, sample liquid reductase 12, the amount of 6- dichloroindophenol titers, with
Ascorbic acid content is directly proportional in sample liquid.
Demarcate ascorbic acid standard liquid：5mL ascorbic acid titers are drawn in triangular flask, and adds 0.5mL6% iodine
Change potassium solution, 3 1% starch solutions of drop, and titrated with the Potassiumiodate titer of 0.001mol/L, occur when titration is reached home blue
Color.Computing formula is as follows：
C-ascorbic acid concentration of standard solution, unit is mg/mL；V1The Potassiumiodate mark of -0.001mol/L for titrating
Quasi- liquid consumes volume, and unit is mL；V2- titrated ascorbic acid volume, unit is mL；0.088-1mL0.001mol iodine
Equivalent to the amount of ascorbic acid, unit is mg/mL to sour potassium titer.Demarcate 2,6 dichlorophenol indophenol solution：Take the anti-of 5mL Jing demarcation
Bad hematic acid titer, and add 5mL1% oxalic acid solutions (to add oxalic acid to prevent ascorbic acid in experimentation by oxidation shadow
Ring experimental result), colour-fast as terminal in pink, and 15s is titrated to by 2,6- dichloroindophenol solution after shaking up.Calculate public
Formula is as follows：
Equivalent to the amount of ascorbic acid, unit is mg/mL to T-per milliliter 2,6- dichloroindophenol solution；
C-ascorbic acid concentrations, unit mg/mL；
V1- ascorbic acid titer volume, unit is mL；
V2- it is used for 2, the 6- dichloroindophenol solution consumption volumes that titrate, unit is m.
Final titration：50mL fruit juice is taken, 2% oxalic acid is added, is shaken up.Accurately draw the fruit that 10mL adds 2% oxalic acid solution
Juice is placed in 50mL triangular flasks, sample liquid is titrated using 2,6- dichloroindophenols solution rapidly, when redness cannot disappear immediately in triangular flask
When, slow down rate of titration, do not taken off as titration end-point in 15s with pink, blank test is done with this simultaneously.Computing formula is such as
Under：
Ascorbic acid content in X-sample liquid, unit is mg/100g；
Equivalent to the amount of ascorbic acid, unit is mg/mL to T-per milliliter 2,6- dichloroindophenol solution；V-titration sample liquid disappears
The volume of consumption 2,6- dichloroindophenol solution, unit is mL；
The blank volume for consuming 2,6- dichloroindophenol solution of V0-titration, unit is mL；
The sample liquid quality of m-titrated, unit is g.
(3) measure of polyphenol content-forint-Xiao Kafa
Principle：In the basic conditions, the polyphenols and folin solution reaction for carrying oxidizable hydroxyl is presented blue, many
Aldehydes matter content is directly proportional to blue depth degree, and the blue material for generating has absorption maximum under 765nm wavelength.From
Thing determines polyphenol content in fruit juice on the basis of gallic acid titer.
Calibration curve：250,150,125,100,75, the 50 and 0mg/L each 1mL of gallic acid titer is taken with pipette,
15% sodium carbonate liquor 15mL is separately added into simultaneously, and Folin reagent 5mL and 60mL water stands colour developing 2 hours after 765nm wavelength
Bioassay standard solution absorbance, and draw calibration curve.
Sample determining total phenol：Take 10mL samples of juice and be diluted to 150mL, take 1mL dilutions sample liquid and determine as stated above.
(4) measure (OD values) of colour
From 1cm glass cuvettes, while making reference with distilled water.
(5) measure of the anthocyanin to hydroxyl radical free radical (OH) Scavenging activity
Fenton reacts spectrophotometric principles：OH in fruit juice can be by Phen-Fe2+Solution oxide is into adjacent phenodiazine
Phenanthrene-Fe3+, make Phen-Fe2+Maximum absorption band at wavelength 536nm disappears, oxygen in the change reflection fruit juice of its color
Change the change of reducing condition, and by corresponding absorbance come OH contents in counting system.
The measure of sample absorbance takes 1mL fruit juice in test tube, and it is molten to add the Phen of the 0.005mol/L of 0.6mL
Liquid, the phosphate solution that the p H of 0.4mL are 7.4, the 0.01mol/L copperas solutions of 0.3mL, 0.6mL distilled water, 0.3mL
0.02mol/L EDTA solution and 0.8mL volume fraction be 0.1% hydrogen peroxide, fully shake up under the conditions of 37 DEG C
Insulation 1h, the mensuration absorbance in the case where wavelength is 536nm.
Blank sample absorbance:70% ethanol solution of 1mL replaces 1mL fruit juice, and 0.8mL distilled water replaces the 0.1% of 0.8mL
Hydrogen peroxide, remaining solution constitutes blank sample with sample identical sample.
Loss absorbance：1mL volume fractions are that 70% ethanol solution correspondence absorbance is as lost part absorbance.
OH clearance rates are with formula calculating：
W=(ASample-ALoss)/(AIt is blank-ALoss)×100
W-OH clearance rates, unit is %；
ASample- sample OD values；
ALoss- loss OD values；
AIt is blank- blank sample OD values.
(6) soluble solid content is determined：Abbe's refractometer
The passion fruit juice drinks prepared according to embodiment 4, carry out according to the method described above Physico-chemical tests, obtain as follows
The measurement result of table 3.
The passion fruit juice drinks characteristic of table 3
Above-mentioned embodiment is the invention is not limited in, if the various changes or deformation to the present invention are without departing from the present invention
Spirit and scope, if these change and deformation belong to the present invention claim and equivalent technologies within the scope of, then this
It is bright to be also intended to comprising these changes and deform.
Claims (10)
1. the extracting method of passion fruit anthocyanin, it is characterised in that：Step is as follows：
(1) passion fruit and pericarp, drying are chosen；
(2) screen, remove the passion fruit and pericarp of debris and brown stain；
(3) crush；
(4) passion fruit and pericarp mixture of crushing are carried out into normal temperature high voltage extraction：Extractant is ethanol solution, passion fruit and fruit
Skin mixture is 1 with the weight ratio of extractant：20, pressure 150Mpa is set, the time is 20～60min, and suction filtration retains filtrate；
(5) filter, obtain passion fruit anthocyanin leaching liquor；
(6) drying concentrated in vacuo, obtains passion fruit anthocyanin.
2. the extracting method of passion fruit anthocyanin according to claim 1, it is characterised in that：The drying condition of step (1)
It is to dry 12 hours at 40 DEG C.
3. the extracting method of passion fruit anthocyanin according to claim 1, it is characterised in that：Step (3) crosses 20 after crushing
Mesh sieve.
4. the extracting method of passion fruit anthocyanin according to claim 1, it is characterised in that：Step (4) extractant
For 80% ethanol.
5. the extracting method of passion fruit anthocyanin according to claim 1, it is characterised in that：Step (6) it is concentrated in vacuo
It is dried specifically：Passion fruit anthocyanin leaching liquor is positioned over into traditional vacuum concentrating instrument to ethanol volatilization completely, by concentrate
It is spray-dried, 130 DEG C of EAT, 80 DEG C of leaving air temp, feeding temperature is 50 DEG C.
6. application of the passion fruit anthocyanin that prepared by claim 1 methods described in passion fruit juice drinks.
7. the preparation method of passion fruit juice drinks, it is characterised in that：Step is as follows：
(1) choosing fruit：Select unabroken new foresythia passion fruit；
(2) squeeze the juice：The pulp of passion fruit is dug out and is put into rapidly in mixer, is stirred at 30 DEG C of temperature control；
(3) digest：The pectase of 0.25% pulp weight is added, at 45 DEG C 2.5h is digested, obtain Passion Fruit Juice；
(4) colloid mill grinding：Passion Fruit Juice is ground by colloid mills twice；
(5) reconcile：After passion fruit anthocyanin prepared by claim 1 methods described is dissolved in water, and stabilizer, honey and Vc
Pour crutcher mediation into together；
(6) homogeneous：Homogeneous 1min under the conditions of 50MPa, homogeneous is once；
(7) ultra high temperature short time sterilization：5～8 seconds plus thermal sterilization are kept at 130 DEG C -150 DEG C, is then cooled down rapidly；
(8) it is canned：Fill empty bottle, stand after 1min immediately with being originally water-cooled to normal temperature.
8. the preparation method of passion fruit juice drinks according to claim 7, it is characterised in that：In step (5), relative to
Passion fruit juice drinks gross weight, the weight percentage for being added water is 65%, and the weight percentage of stabilizer is 1.22%, honeybee
The weight percentage of honey is 1% for the weight percentage of 2.4%, Vc.
9. the preparation method of passion fruit juice drinks according to claim 7, it is characterised in that：Stirring in step (2) turns
Speed is 70 revs/min.
10. passion fruit juice drinks, it is characterised in that：Obtained using the preparation method described in claim 7.
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| CN109673876A (en) * | 2019-01-24 | 2019-04-26 | 广西古岭龙食品有限公司 | The preparation method of Passion Fruit Juice beverage |
| JP2019137818A (en) * | 2018-02-15 | 2019-08-22 | 国立大学法人 鹿児島大学 | Red dye contained in passion fruit pericarp and method for producing the same |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2019137818A (en) * | 2018-02-15 | 2019-08-22 | 国立大学法人 鹿児島大学 | Red dye contained in passion fruit pericarp and method for producing the same |
| JP7021773B2 (en) | 2018-02-15 | 2022-02-17 | 国立大学法人 鹿児島大学 | Red pigment contained in passion fruit peel and its manufacturing method |
| CN108864735A (en) * | 2018-06-01 | 2018-11-23 | 常州市艺染生物科技有限公司 | A kind of extracting method and its application of passion fruit pigment |
| CN109673876A (en) * | 2019-01-24 | 2019-04-26 | 广西古岭龙食品有限公司 | The preparation method of Passion Fruit Juice beverage |
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