CN102757664A - Extracting method of passion flower fruit pigment - Google Patents
Extracting method of passion flower fruit pigment Download PDFInfo
- Publication number
- CN102757664A CN102757664A CN2012102261774A CN201210226177A CN102757664A CN 102757664 A CN102757664 A CN 102757664A CN 2012102261774 A CN2012102261774 A CN 2012102261774A CN 201210226177 A CN201210226177 A CN 201210226177A CN 102757664 A CN102757664 A CN 102757664A
- Authority
- CN
- China
- Prior art keywords
- pigment
- purification
- extracting
- massfraction
- vacuum concentration
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
The invention discloses an extracting method of passion flower fruit pigment. The extracting method comprises the steps of selecting materials, cleaning, draining, extracting ethanol, extracting absolute ether, extracting ethyl acetate, absorbing and purifying macroporous resin, absorbing and purifying polyamide chromatographic column, absorbing and purifying sephadex columns, separating and purifying preparative liquid chromatography, cooling, drying and packaging. According to the method, the operation is simple, a used solvent and an absorbent can be used repeatedly, the energy loss is low, the real-time control is easy, and the continuous automatic cleaning production can be realized.
Description
Technical field
The invention belongs to the research and development of natural products technical field, be specifically related to a kind of process for extracting of Herba Passiflorae Caeruleae fruit pigment.
Background technology
Herba Passiflorae Caeruleae is the important tropical fruit of China, and output is bigger, and Herba Passiflorae Caeruleae is mainly used in manufacturing fruit juice at present, and few part is edible as fresh fruit.The Herba Passiflorae Caeruleae pericarp accounts for whole fruit and weighs 45%～55%, in a large amount of dahlia fruit juice processing, becomes by product, and these processing byproduct major parts are used as waste material and abandon, and the significant wastage that this has not only caused resource also causes burden to environment.Purple fruit Herba Passiflorae Caeruleae pericarp contains abundant natural pigment, is expected to obtain the product of high extraction, high purity, high luminance relay valency, to satisfy the demand of domestic and international market to natural pigment.
Summary of the invention
The object of the present invention is to provide a kind of process for extracting of Herba Passiflorae Caeruleae fruit pigment; The present invention is simple to operate, and solvent for use and sorbent material all can reuse, and energy consumption is little; And be easy to instant control; Can realize the continuous and automatic cleaner production, obtain the pure article of the higher passion fruit skin pigment of purity, product purity reaches more than 90%.
For realizing above-mentioned purpose, the present invention adopts following technical scheme:
A kind of process for extracting of Herba Passiflorae Caeruleae fruit pigment comprises: select materials, clean, drain, extraction using alcohol, anhydrous diethyl ether extraction, ethyl acetate extraction, macroporous resin adsorption purifying, polymeric amide chromatography post adsorption and purification, sephadex column adsorption and purification, preparative liquid chromatography separation and purification, lyophilize, packing.
The concrete steps of said process for extracting are following:
(1) select materials: with the Herba Passiflorae Caeruleae pericarp is raw material;
(2) clean: clean with flowing water after removing impurity;
(3) drain: the Herba Passiflorae Caeruleae pericarp after will cleaning is divided on the bamboo sieve and is drained;
(4) extraction using alcohol: adopting massfraction is that 86% ethanol, massfraction are that 0.054%HCl and massfraction are that 0.1% Hydrocerol A mixing solutions extracts pigment, extract finish after, 40 ℃ of vacuum concentration are removed ethanol, pigment concentrate;
(5) anhydrous diethyl ether extraction: in pigment concentrate, for adding anhydrous diethyl ether, 1:1 extracts by volume, the water intaking layer, vacuum concentration is removed ether, and is for several times colourless until ether layer repeatedly;
(6) ethyl acetate extraction: by volume for 1:1 adds ethyl acetate extraction, the water intaking layer, vacuum concentration is removed ETHYLE ACETATE, and is for several times colourless until ethyl acetate layer repeatedly;
(7) macroporous resin adsorption purifying: select the HP-20 macroporous resin for use; Wet method upper prop after pre-treatment is transferred pigment solution pH to 3.5 with Hydrogen chloride, passes through adsorption column with constant flow pump control pigment solution; Treat the effluent light absorption value be the sample solution light absorption value 10% after stop to add pigment solution; Dividing with pure water and to clean resin surface for several times, removing part sugar, protein, is that 3.5 massfraction is that 90% ethanolic soln is an eluent wash-out pigment solution with pH; The pigment solution that obtains adopts 40 ℃ of vacuum concentration to remove ethanol, obtains the preliminary purification pigment solution;
(8) polymeric amide chromatography post adsorption and purification: polymeric amide is wet method dress post after pre-treatment; Purification of samples is crossed polymeric amide chromatography post; With massfraction is that 50% methyl alcohol and massfraction are that 0.1% formic acid mixing solutions is an eluent, collects coloured moiety, the elutriant vacuum concentration;
(9) sephadex column adsorption and purification: polydextran gel is wet method dress post after pre-treatment; Purification of samples is crossed sephadex column; With massfraction is that 50% methyl alcohol and massfraction are that 0.1% formic acid mixing solutions is an eluent, collects coloured moiety, the elutriant vacuum concentration;
(10) preparative liquid chromatography separation and purification: adopt preparative liquid chromatography to the pigment solution separation and purification after concentrating, obtain passion fruit skin pigment monomer;
(11) lyophilize: adopt Freeze Drying Technique, fling to the moisture of pigment solution, obtain the pure powder of pigment;
(12) packing: adopt powder series products universaling packing mode to pack.
Described Herba Passiflorae Caeruleae pericarp is the by product of dahlia fruit juice processing.
Adopt preparative liquid chromatography separation and purification passion fruit skin pigment monomer, working conditions is: F door LUNA-C18 chromatographic column, 4.6mm * 150mm * 5 μ; Sample size 10 μ L, flow velocity 0.4 mL/min, sweep limit: 254nm-600 nm; Detect wavelength 520 nm, moving phase is 4.5% formic acid A, methyl alcohol B; Condition of gradient elution: 0min, 10%B; 30min, 25%B; 40 min, 45%B; 42 min, 90%B; 45 min, 10%B, gained pigment sample purity is high.
Beneficial effect of the present invention:
(1) adopt alcohol solvent to extract the passion fruit skin pigment, have economy, convenience, nontoxic, be easy to get, advantage such as reusable edible.
(2) adopt anhydrous diethyl ether and ETHYLE ACETATE to extract the pigment fluid power repeatedly and effectively remove oil-soluble impurities in the pigment solution.
(3) system adopts macroporous resin column chromatography, polyamide column chromatography, the polydextran gel column chromatography plain liquid of checking colors to carry out adsorption and purification, can effectively remove water-soluble impurity in the pigment solution, realizes the monomeric initial gross separation of pigment.
(4) adopt preparative liquid chromatography to carry out the separation and purification of passion fruit skin pigment, can effectively realize the monomeric separation of passion fruit skin pigment, guaranteed the purity of finished product.
(5) the utilization Freeze Drying Technique obtains the pure powder of pigment, the destruction of having avoided high temperature to cause pigment.
Embodiment
Below be several specific embodiment of the present invention, further specify the present invention, but the present invention is not limited only to this embodiment.The process for extracting of a kind of passion fruit skin pigment of the present invention, concrete steps are following:
Embodiment 1
(1) select materials: with the Herba Passiflorae Caeruleae pericarp is raw material;
(2) clean: clean with flowing water after removing impurity;
(3) drain: the Herba Passiflorae Caeruleae pericarp after will cleaning is divided on the bamboo sieve and is drained;
(4) alcohol solvent extracts: adopt 86% ethanol (containing 0.054% hydrochloric acid, 0.1% Hydrocerol A) to extract pigment, extract 31 ℃ of temperature, and times 120 min, solid-liquid ratio 1:36, after extraction finished, 40 ℃ of vacuum concentration were removed ethanol;
(5) anhydrous diethyl ether extraction: the ratio in 1:1 (v/v) in pigment concentrate adds the anhydrous diethyl ether extraction, the water intaking layer, and vacuum concentration is removed ether, and is for several times colourless until ether layer repeatedly;
(6) ethyl acetate extraction: the ratio in 1:1 (v/v) adds ethyl acetate extraction, the water intaking layer, and vacuum concentration is removed ETHYLE ACETATE, and is for several times colourless until ethyl acetate layer repeatedly;
(7) macroporous resin adsorption purifying: select the HP-20 macroporous resin for use, wet method upper prop after pre-treatment is transferred pigment solution pH to 3.5 with Hydrogen chloride; With constant flow pump control pigment solution with the speed of 1BV/h through adsorption column, treat the effluent light absorption value be the sample solution light absorption value 10% after stop to add pigment solution, divide several to clean resin surface with the 4BV pure water; To remove water-soluble substanceses such as part sugar, protein; 90% ethanolic soln with pH3.5 is an eluent wash-out pigment solution, eluent consumption 6BV, elution speed 2BV/h; The pigment solution that obtains adopts 40 ℃ of vacuum concentration to remove ethanol, obtains the preliminary purification pigment solution;
(8) polymeric amide chromatography post adsorption and purification: polymeric amide is wet method dress post after pre-treatment, and purification of samples is crossed polymeric amide chromatography post, is eluent with 50% methyl alcohol (containing 0.1% formic acid), collects coloured moiety, the elutriant vacuum concentration.
(9) sephadex column adsorption and purification: polydextran gel is wet method dress post after pre-treatment, and purification of samples is crossed sephadex column, is eluent with 50% methyl alcohol (containing 0.1% formic acid), collects coloured moiety, the elutriant vacuum concentration.
(10) preparative liquid chromatography separation and purification: adopt preparative liquid chromatography to the pigment solution separation and purification after concentrating, obtain passion fruit skin pigment monomer.Working conditions is: F door LUNA-C18 chromatographic column (4.6mm * 150mm * 5 μ), sample size 10 μ L, flow velocity 0.4 mL/min; Sweep limit: 254nm-600 nm, detect wavelength 520 nm, moving phase is 4.5% formic acid (A); Methyl alcohol (B), condition of gradient elution: 0min, 10%B; 30min, 25%B; 40 min, 45%B; 42 min, 90%B; 45 min, 10%B.
(11) lyophilize: adopt Freeze Drying Technique, fling to the moisture of pigment solution at a lower temperature, obtain the pure powder of pigment; The control temperature of heating plate is 50 ℃; Material bed thickness 4mm, drying chamber pressure 60 Pa, dry with this understanding 12 h can be reduced to moisture content of material below 8%;
(12) packing: adopt three layers of clad aluminum foil bag wrapped product.
Embodiment 2
(1) select materials: with the Herba Passiflorae Caeruleae pericarp is raw material;
(2) clean: clean with flowing water after removing impurity;
(3) drain: the Herba Passiflorae Caeruleae pericarp after will cleaning is divided on the bamboo sieve and is drained;
(4) alcohol solvent extracts: adopt 86% ethanol (containing 0.054% hydrochloric acid, 0.1% Hydrocerol A) to extract pigment, extract temperature normal temperature, and times 140 min, solid-liquid ratio 1:30, after extraction finished, 40 ℃ of vacuum concentration were removed ethanol;
(5) anhydrous diethyl ether extraction: the ratio in 1:1 (v/v) in pigment concentrate adds the anhydrous diethyl ether extraction, the water intaking layer, and vacuum concentration is removed ether, and is for several times colourless until ether layer repeatedly;
(6) ethyl acetate extraction: the ratio in 1:1 (v/v) adds ethyl acetate extraction, the water intaking layer, and vacuum concentration is removed ETHYLE ACETATE, and is for several times colourless until ethyl acetate layer repeatedly;
(7) macroporous resin adsorption purifying: select the HP-20 macroporous resin for use, wet method upper prop after pre-treatment is transferred pigment solution pH to 3.5 with Hydrogen chloride; With constant flow pump control pigment solution with the speed of 2BV/h through adsorption column, treat the effluent light absorption value be the sample solution light absorption value 10% after stop to add pigment solution, divide several to clean resin surface with the 6BV pure water; To remove water-soluble substanceses such as part sugar, protein; 90% ethanolic soln with pH3.5 is an eluent wash-out pigment solution, eluent consumption 6BV, elution speed 2BV/h; The pigment solution that obtains adopts 40 ℃ of vacuum concentration to remove ethanol, obtains the preliminary purification pigment solution;
(8) polymeric amide chromatography post adsorption and purification: polymeric amide is wet method dress post after pre-treatment, and purification of samples is crossed polymeric amide chromatography post, is eluent with 50% methyl alcohol (containing 0.1% formic acid), collects coloured moiety, the elutriant vacuum concentration.
(9) sephadex column adsorption and purification: polydextran gel is wet method dress post after pre-treatment, and purification of samples is crossed sephadex column, is eluent with 50% methyl alcohol (containing 0.1% formic acid), collects coloured moiety, the elutriant vacuum concentration.
(10) preparative liquid chromatography separation and purification: adopt preparative liquid chromatography to the pigment solution separation and purification after concentrating, obtain passion fruit skin pigment monomer.Working conditions is: F door LUNA-C18 chromatographic column (4.6mm * 150mm * 5 μ), sample size 10 μ L, flow velocity 0.4 mL/min; Sweep limit: 254nm-600 nm, detect wavelength 520 nm, moving phase is 4.5% formic acid (A); Methyl alcohol (B), condition of gradient elution: 0min, 10%B; 30min, 25%B; 40 min, 45%B; 42 min, 90%B; 45 min, 10%B.
(11) lyophilize: adopt Freeze Drying Technique, fling to the moisture of pigment solution at a lower temperature, obtain the pure powder of pigment; The control temperature of heating plate is 50 ℃; Material bed thickness 4mm, drying chamber pressure 60 Pa, dry with this understanding 12 h can be reduced to moisture content of material below 8%;
(12) packing: adopt three layers of clad aluminum foil bag wrapped product.
Embodiment 3
(1) select materials: with the Herba Passiflorae Caeruleae pericarp is raw material;
(2) clean: clean with flowing water after removing impurity;
(3) drain: the Herba Passiflorae Caeruleae pericarp after will cleaning is divided on the bamboo sieve and is drained;
(4) alcohol solvent extracts: adopt 86% ethanol (containing 0.054% hydrochloric acid, 0.1% Hydrocerol A) to extract pigment, extract temperature normal temperature, and times 90 min, solid-liquid ratio 1:40, after extraction finished, 40 ℃ of vacuum concentration were removed ethanol;
(5) anhydrous diethyl ether extraction: the ratio in 1:1 (v/v) in pigment concentrate adds the anhydrous diethyl ether extraction, the water intaking layer, and vacuum concentration is removed ether, and is for several times colourless until ether layer repeatedly;
(6) ethyl acetate extraction: the ratio in 1:1 (v/v) adds ethyl acetate extraction, the water intaking layer, and vacuum concentration is removed ETHYLE ACETATE, and is for several times colourless until ethyl acetate layer repeatedly;
(7) macroporous resin adsorption purifying: select the HP-20 macroporous resin for use, wet method upper prop after pre-treatment is transferred pigment solution pH to 3.5 with Hydrogen chloride; With constant flow pump control pigment solution with the speed of 2BV/h through adsorption column, treat the effluent light absorption value be the sample solution light absorption value 10% after stop to add pigment solution, divide several to clean resin surface with the 6BV pure water; To remove water-soluble substanceses such as part sugar, protein; 90% ethanolic soln with pH3.5 is an eluent wash-out pigment solution, eluent consumption 6BV, elution speed 3BV/h; The pigment solution that obtains adopts 40 ℃ of vacuum concentration to remove ethanol, obtains the preliminary purification pigment solution;
(8) polymeric amide chromatography post adsorption and purification: polymeric amide is wet method dress post after pre-treatment, and purification of samples is crossed polymeric amide chromatography post, is eluent with 50% methyl alcohol (containing 0.1% formic acid), collects coloured moiety, the elutriant vacuum concentration.
(9) sephadex column adsorption and purification: polydextran gel is wet method dress post after pre-treatment, and purification of samples is crossed sephadex column, is eluent with 50% methyl alcohol (containing 0.1% formic acid), collects coloured moiety, the elutriant vacuum concentration.
(10) preparative liquid chromatography separation and purification: adopt preparative liquid chromatography to the pigment solution separation and purification after concentrating, obtain passion fruit skin pigment monomer.Working conditions is: F door LUNA-C18 chromatographic column (4.6mm * 150mm * 5 μ), sample size 10 μ L, flow velocity 0.4 mL/min; Sweep limit: 254nm-600 nm, detect wavelength 520 nm, moving phase is 4.5% formic acid (A); Methyl alcohol (B), condition of gradient elution: 0min, 10%B; 30min, 25%B; 40 min, 45%B; 42 min, 90%B; 45 min, 10%B.
(11) lyophilize: adopt Freeze Drying Technique, fling to the moisture of pigment solution at a lower temperature, obtain the pure powder of pigment; The control temperature of heating plate is 40 ℃; Material bed thickness 4mm, drying chamber pressure 60 Pa, dry with this understanding 18 h can be reduced to moisture content of material below 8%;
(12) packing: adopt three layers of clad aluminum foil bag wrapped product.
The above is merely preferred embodiment of the present invention, and all equalizations of doing according to claim of the present invention change and modify, and all should belong to covering scope of the present invention.
Claims (4)
1. the process for extracting of a Herba Passiflorae Caeruleae fruit pigment, it is characterized in that: the step of said process for extracting comprises: select materials, clean, drain, extraction using alcohol, anhydrous diethyl ether extraction, ethyl acetate extraction, macroporous resin adsorption purifying, polymeric amide chromatography post adsorption and purification, sephadex column adsorption and purification, preparative liquid chromatography separation and purification, lyophilize, packing.
2. the process for extracting of Herba Passiflorae Caeruleae fruit pigment according to claim 1 is characterized in that: the concrete steps of said process for extracting are following:
(1) select materials: with the Herba Passiflorae Caeruleae pericarp is raw material;
(2) clean: clean with flowing water after removing impurity;
(3) drain: the Herba Passiflorae Caeruleae pericarp after will cleaning is divided on the bamboo sieve and is drained;
(4) extraction using alcohol: adopting massfraction is that 86% ethanol, massfraction are that 0.054%HCl and massfraction are that 0.1% Hydrocerol A mixing solutions extracts pigment, extract finish after, 40 ℃ of vacuum concentration are removed ethanol, pigment concentrate;
(5) anhydrous diethyl ether extraction: in pigment concentrate, for adding anhydrous diethyl ether, 1:1 extracts by volume, the water intaking layer, vacuum concentration is removed ether, and is for several times colourless until ether layer repeatedly;
(6) ethyl acetate extraction: by volume for 1:1 adds ethyl acetate extraction, the water intaking layer, vacuum concentration is removed ETHYLE ACETATE, and is for several times colourless until ethyl acetate layer repeatedly;
(7) macroporous resin adsorption purifying: select the HP-20 macroporous resin for use; Wet method upper prop after pre-treatment is transferred pigment solution pH to 3.5 with Hydrogen chloride, passes through adsorption column with constant flow pump control pigment solution; Treat the effluent light absorption value be the sample solution light absorption value 10% after stop to add pigment solution; Dividing with pure water and to clean resin surface for several times, removing part sugar, protein, is that 3.5 massfraction is that 90% ethanolic soln is an eluent wash-out pigment solution with pH; The pigment solution that obtains adopts 40 ℃ of vacuum concentration to remove ethanol, obtains the preliminary purification pigment solution;
(8) polymeric amide chromatography post adsorption and purification: polymeric amide is wet method dress post after pre-treatment; Purification of samples is crossed polymeric amide chromatography post; With massfraction is that 50% methyl alcohol and massfraction are that 0.1% formic acid mixing solutions is an eluent, collects coloured moiety, the elutriant vacuum concentration;
(9) sephadex column adsorption and purification: polydextran gel is wet method dress post after pre-treatment; Purification of samples is crossed sephadex column; With massfraction is that 50% methyl alcohol and massfraction are that 0.1% formic acid mixing solutions is an eluent, collects coloured moiety, the elutriant vacuum concentration;
(10) preparative liquid chromatography separation and purification: adopt preparative liquid chromatography to the pigment solution separation and purification after concentrating, obtain passion fruit skin pigment monomer;
(11) lyophilize: adopt Freeze Drying Technique, fling to the moisture of pigment solution, obtain the pure powder of pigment;
(12) packing: adopt powder series products universaling packing mode to pack.
3. the process for extracting of Herba Passiflorae Caeruleae fruit pigment according to claim 2 is characterized in that: described Herba Passiflorae Caeruleae pericarp is the by product of dahlia fruit juice processing.
4. the process for extracting of Herba Passiflorae Caeruleae fruit pigment according to claim 2 is characterized in that: adopt preparative liquid chromatography separation and purification passion fruit skin pigment monomer, working conditions is: F door LUNA-C18 chromatographic column; 4.6mm * 150mm * 5 μ, sample size 10 μ L, flow velocity 0.4 mL/min; Sweep limit: 254nm-600 nm, detect wavelength 520 nm, moving phase is 4.5% formic acid A; Methyl alcohol B, condition of gradient elution: 0min, 10%B; 30min, 25%B; 40 min, 45%B; 42 min, 90%B; 45 min, 10%B, gained pigment sample purity is high.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012102261774A CN102757664B (en) | 2012-07-03 | 2012-07-03 | Extracting method of passion flower fruit pigment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012102261774A CN102757664B (en) | 2012-07-03 | 2012-07-03 | Extracting method of passion flower fruit pigment |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102757664A true CN102757664A (en) | 2012-10-31 |
| CN102757664B CN102757664B (en) | 2013-11-13 |
Family
ID=47052337
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012102261774A Expired - Fee Related CN102757664B (en) | 2012-07-03 | 2012-07-03 | Extracting method of passion flower fruit pigment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102757664B (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103952012A (en) * | 2014-04-30 | 2014-07-30 | 桂林军供生化技术开发有限公司 | Extraction method of passion flower fruit pigment |
| CN104559304A (en) * | 2014-12-19 | 2015-04-29 | 桂林得坤生物科技股份有限公司 | Method for extracting pigment in persimmon |
| CN104693834A (en) * | 2015-03-31 | 2015-06-10 | 桂林得坤生物科技股份有限公司 | Method for preparing persimmon fruit pigments |
| CN105780058A (en) * | 2014-12-23 | 2016-07-20 | 宁波创润新材料有限公司 | Cleaning method of cathode electrolytic products |
| CN106578825A (en) * | 2016-11-22 | 2017-04-26 | 河源农社食品有限公司 | Extraction method and application of passion fruit anthocyanin and passion fruit juice drink |
| CN107646764A (en) * | 2017-09-30 | 2018-02-02 | 广东海洋大学 | It is a kind of grow cultured pearls before change Pteria penguin mother's shellfish color and luster method and Pteria penguin mother's shellfish color and luster detection method |
| CN108864735A (en) * | 2018-06-01 | 2018-11-23 | 常州市艺染生物科技有限公司 | A kind of extracting method and its application of passion fruit pigment |
| CN110078778A (en) * | 2019-05-15 | 2019-08-02 | 广西壮族自治区中国科学院广西植物研究所 | A method of removing prunasin from passion fruit pericarp crude extract |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101255168A (en) * | 2008-04-09 | 2008-09-03 | 中国农业科学院农产品加工研究所 | Method for preparing liquid chromatography of monascin and monascus anka flavine pure product |
| US20090246343A1 (en) * | 2008-03-28 | 2009-10-01 | Wild Flavors, Inc. | Stable Natural Color Process, Products and Use Thereof |
| WO2010061412A1 (en) * | 2008-11-28 | 2010-06-03 | New Life Hold S.R.L. | New composition for bakery products |
| US20110142775A1 (en) * | 2005-05-31 | 2011-06-16 | Velveteen Bunni Llc | Cosmetic and dermatological formulations with natural pigments and methods of use |
| CN102285949A (en) * | 2011-08-02 | 2011-12-21 | 桂林三宝药业有限公司 | Method for extracting chrysin from passiflora edulis fruit residue obtained after juicing |
-
2012
- 2012-07-03 CN CN2012102261774A patent/CN102757664B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110142775A1 (en) * | 2005-05-31 | 2011-06-16 | Velveteen Bunni Llc | Cosmetic and dermatological formulations with natural pigments and methods of use |
| US20090246343A1 (en) * | 2008-03-28 | 2009-10-01 | Wild Flavors, Inc. | Stable Natural Color Process, Products and Use Thereof |
| CN101255168A (en) * | 2008-04-09 | 2008-09-03 | 中国农业科学院农产品加工研究所 | Method for preparing liquid chromatography of monascin and monascus anka flavine pure product |
| WO2010061412A1 (en) * | 2008-11-28 | 2010-06-03 | New Life Hold S.R.L. | New composition for bakery products |
| CN102285949A (en) * | 2011-08-02 | 2011-12-21 | 桂林三宝药业有限公司 | Method for extracting chrysin from passiflora edulis fruit residue obtained after juicing |
Non-Patent Citations (3)
| Title |
|---|
| 孔秋玲等: "聚酰胺精制紫果西番莲总黄酮的工艺研究", 《安徽农业科学》 * |
| 彭彬等: "西番莲果汁色素提取工艺的研究", 《福建轻纺》 * |
| 陈旭丹等: "西番莲果皮色素Ⅰ稳定性研究", 《食品科学》 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103952012A (en) * | 2014-04-30 | 2014-07-30 | 桂林军供生化技术开发有限公司 | Extraction method of passion flower fruit pigment |
| CN104559304A (en) * | 2014-12-19 | 2015-04-29 | 桂林得坤生物科技股份有限公司 | Method for extracting pigment in persimmon |
| CN105780058A (en) * | 2014-12-23 | 2016-07-20 | 宁波创润新材料有限公司 | Cleaning method of cathode electrolytic products |
| CN104693834A (en) * | 2015-03-31 | 2015-06-10 | 桂林得坤生物科技股份有限公司 | Method for preparing persimmon fruit pigments |
| CN106578825A (en) * | 2016-11-22 | 2017-04-26 | 河源农社食品有限公司 | Extraction method and application of passion fruit anthocyanin and passion fruit juice drink |
| CN107646764A (en) * | 2017-09-30 | 2018-02-02 | 广东海洋大学 | It is a kind of grow cultured pearls before change Pteria penguin mother's shellfish color and luster method and Pteria penguin mother's shellfish color and luster detection method |
| CN108864735A (en) * | 2018-06-01 | 2018-11-23 | 常州市艺染生物科技有限公司 | A kind of extracting method and its application of passion fruit pigment |
| CN110078778A (en) * | 2019-05-15 | 2019-08-02 | 广西壮族自治区中国科学院广西植物研究所 | A method of removing prunasin from passion fruit pericarp crude extract |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102757664B (en) | 2013-11-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102757664B (en) | Extracting method of passion flower fruit pigment | |
| CN104086614B (en) | A kind of preparation method being applicable to industrial Fructus Monordicae extract | |
| CN103467540B (en) | A kind of method extracting rhodioside from Root of Kirilow Rhodiola | |
| CN104558088A (en) | Method for extracting mogroside V from momordica grosvenori | |
| CN101829210B (en) | Method for preparing apple polyphenol | |
| CN105998139B (en) | A method of flavones is extracted from Sweet Potato Leaf | |
| CN104418743B (en) | A kind of method of slightly getting refining chlorogenic acid thing from Japanese Honeysuckle | |
| CN102432535A (en) | Method for extracting and separating huperzine A and huperzine B from huperzia serrata | |
| CN102675910B (en) | Preparation method of high-color-value beet root red color | |
| CN104418741B (en) | A kind of method of chlorogenic acid extracting and purifying from leaf of canada potato | |
| CN104119229A (en) | Technology for producing pure chlorogenic acid | |
| CN105418571B (en) | The method that black carrot anthocyanidin is extracted using microwave counter current | |
| CN104829656B (en) | A kind of method that gentiamarin chemical reference substance is prepared from gentiana straminea | |
| CN106831597A (en) | A kind of method that utilization mushroom leftover bits and pieces prepares natural erythrothioneine | |
| CN102093458A (en) | Method for enriching and purifying betulin in birch barks | |
| CN101475620B (en) | Efficient energy-saving extraction and production method for high-purity cordycepin | |
| CN103664610B (en) | Method for extracting chlorogenic acid from sweet potato leaves | |
| CN103450000B (en) | Method for extracting hypericin from hyperforin perforatum | |
| CN103006824A (en) | Method for extracting tritepenoidic acid from jujube leaves | |
| CN101058570B (en) | Process of resin and gel multi-stage series connection high efficiency integration separation and purification of apple polyphenol | |
| CN103232503A (en) | Method for preparing verbenalin | |
| CN102731251B (en) | Purification method of highly pure organic solvent n-butanol | |
| CN104193668A (en) | Method for extracting L-tryptophan from fermentation liquor through macroporous resin | |
| CN104926904B (en) | A kind of method of extraction purification cordycepin in mycoderma from Cordyceps militaris | |
| CN103058882A (en) | Method for separating natural theanine |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20131113 Termination date: 20180703 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |