CN105830698A - Seedling hardening method for sweet potato tissue-cultured test-tube plantlets - Google Patents
Seedling hardening method for sweet potato tissue-cultured test-tube plantlets Download PDFInfo
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- CN105830698A CN105830698A CN201610187666.1A CN201610187666A CN105830698A CN 105830698 A CN105830698 A CN 105830698A CN 201610187666 A CN201610187666 A CN 201610187666A CN 105830698 A CN105830698 A CN 105830698A
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- seedling
- hardening
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- sweet potato
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
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- Developmental Biology & Embryology (AREA)
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Abstract
The invention relates to seedling hardening methods for tissue-cultured test-tube plantlets and particularly relates to a seedling hardening method for sweet potato tissue-cultured test-tube plantlets. The seedling hardening method is exclusively applied to the seedling-hardened transplanting of the sweet potato tissue-cultured test-tube plantlets. The seedling hardening method for the sweet potato tissue-cultured test-tube plantlets, disclosed by the invention, comprises the steps of carrying out in-bottle seedling hardening and carrying out matrix seedling hardening, wherein the in-bottle seedling hardening comprises the step of opening bottle caps so as to acclimatize the sweet potato plantlets for 4 to 7 days; and the matrix seedling hardening comprises the steps of cleaning a culture medium adhered to roots of the sweet potato plantlets subjected to the in-bottle seedling hardening with clean water, soaking the roots with a disinfecting solution, and then, planting the sweet potato plantlets in matrix seedling beds for seedling hardening. The method has the advantages that the technique is simple, the operation is facilitated, the transplanting survival rate of hardened seedlings is high, the field-planted seedling restitution speed is high, and the like; and proven by practice, the implementation effect is good, so that the method can be excellently popularized in production.
Description
Technical field
The present invention relates to the hardening off method of group training test tube Seedling, particularly to Rhizoma Dioscoreae esculentae group training test tube Seedling
Hardening off method, is exclusively used in the acclimatization and transplants of Rhizoma Dioscoreae esculentae group training test tube Seedling.
Background technology
China is Rhizoma Dioscoreae esculentae manufacturing country maximum in the world, and China's Sweet Potato Industry accounts for the world about 60%
Cultivated area, results the Liao Zhan world always produce about 80% yield.Rhizoma Dioscoreae esculentae has good medicine
With being worth and nutritive value, Rhizoma Dioscoreae esculentae nature and flavor are sweet flat, nontoxic, can tonifying the spleen and stomach, nourishing the heart to keep a sound mind, QI invigorating
Power, promoting lactation, except place stasis of blood dysentery.Nutrient sweet potato enrich, rich in starch, saccharide, protein,
Vitamin, cellulose and various aminoacid, be extraordinary nutraceutical.
Owing to asexual propagation for many years and insecticide pass poison, Rhizoma Dioscoreae esculentae is made to be affected by multiple virus,
Inhibit the performance of growth of sweet potato, cause kind of a sexual involution, drastically influence yield and quality.Logical
Cross stem-tip tissue to cultivate, breed group training detoxification test tube plantlet, utilize test tube transplantation of seedlings Production of Large Fields to take off
Poison Rhizoma Dioscoreae esculentae, recovers Rhizoma Dioscoreae esculentae kind, is raising yield of sweet potato, improves the important technology of Rhizoma Dioscoreae esculentae quality
One of measure.But during test tube transplantation of seedlings Production of Large Fields, due to Rhizoma Dioscoreae esculentae group training test tube Seedling children
Tender, root system is more weak, and regeneration capacity is poor, and microenvironment and land for growing field crops ring in bottle before and after test tube transplantation of seedlings
Border condition difference is relatively big, and the test tube shoot survival percent often leading to transplant land for growing field crops is relatively low, even if surviving
Seedling, it is slow to go back Seedling speed, is unfavorable for expanding of Roots Neck of Sweet Potato.Seedling exercising substrate and environmental condition
Being the key problem of seedling exercising technology, whether seedling exercising technology reaches a standard, and is to limit industrial seedling rearing flowing water
The key that can line run, and seedling exercising survival rate height, eventually affect the production scale of product.
Summary of the invention
It is an object of the invention to provide a kind of can aborning popularization and application Rhizoma Dioscoreae esculentae group training test tube
Seedling acclimatization and transplants method.
The hardening off method of a kind of Rhizoma Dioscoreae esculentae group training test tube Seedling, comprises the following steps:
1) in-bottle seeding: Rhizoma Dioscoreae esculentae group training test tube Seedling is placed in temperature 20～30 DEG C, light intensity 15～
28klx, illumination 10～12h/d, 70～80% humidity airtight greenhouse in, open bottle cap, make
Adaptation environment 4～7d, in-bottle seeding can be completed；
2) substrate seedling exercising: by step 1) taking-up of gained test tube Seedling, clean Rhizoma Dioscoreae esculentae root system with clear water
The culture medium adhered to, plants in substrate seedbed, with plastic sheeting pair after soaking with antiseptic solution
Seedbed covers,
Described substrate by loam 2500～3000kg, coconut palm bran 30～50kg, perlite 30～
50kg, carbamide 200～250kg, potassium dihydrogen phosphate 180～200kg, polyacrylamide 5～10kg,
The stirring of activated carbon 3～5kg, anti virus herb extract 3～5kg mixes,
Described anti virus herb compositions is prepared by following methods: take 10～15 weight portions
Herba Rosmarini Officinalis, 10～15 weight portion Adeps Bovis seu Bubalis, 8～10 weight portion Herba Pogostemoniss, 5～10 weight portion Rhizoma Coptidis,
3～5 weight portion Radix Bupleuri, 3～5 weight portion Spica Prunellaes, add 10～12 times of weight parts waters decoct, and receive
Collection filtrate also concentrates, and is dried to obtain anti virus herb extract.
Coconut palm bran described in preferably: step 2) is the by-product after Cortex cocois radicis processing.
Antiseptic solution described in preferably: step 2) is the carbendazim solution of 1000 times, institute
The soak time stated is 5～10min.
Water content of substrate described in preferably: step 2) is 75%～85% mass fraction.
Substrate seedling exercising described in preferably: step 2) is to carry out in airtight greenhouse, base
Matter seedling exercising is in the 1st～3 day totally 3 day time, and seedling exercising environmental condition is: 24～28 DEG C daytime temperature
Intensity of illumination at noon, 75%～80% of degree, 17～20 DEG C of nocturnal temperatures, 25～35klx
Air humidity.
Substrate seedling exercising described in preferably: step 2) from the 4th day, seedling exercising environmental condition
For: 22～30 DEG C daytime temperature, 15～22 DEG C of nocturnal temperatures, 20～the illumination at noon of 40klx
The air humidity of intensity, 70%～90%.
Substrate seedling exercising described in preferably: step 2), from the 15th day, removes seedbed thin
Film.
Substrate seedling exercising described in preferably: step 2) from the 22nd～25 day, Rhizoma Dioscoreae esculentae Seedling
Land for growing field crops can be transplanted, complete substrate seedling exercising.
The Rhizoma Dioscoreae esculentae hardening off method that the present invention provides has the following characteristics that
It is simple, convenient to operate that the present invention has technology, and acclimatization and transplants survival rate is high, land for growing field crops of planting
After go back the advantages such as Seedling is fast, in practice it has proved that, implementation result is good, can promote the most aborning
Application.
Detailed description of the invention
Technical scheme is described further below, but claimed scope is not limited to
In described.Below in conjunction with specific embodiment, the present invention is made further details of elaboration, but this
The embodiment of invention is not limited to the scope that embodiment represents.These embodiments are only used for
The bright present invention, not for limiting the scope of the present invention.Additionally, reading present disclosure
After, those skilled in the art can various modifications may be made to the present invention, and these equivalent variations are same
Fall within appended claims limited range of the present invention.
Embodiment 1
The hardening off method of Rhizoma Dioscoreae esculentae group training test tube Seedling includes in-bottle seeding and two stages of substrate seedling exercising:
1) in-bottle seeding: Rhizoma Dioscoreae esculentae group training test tube Seedling is placed in temperature 20 DEG C, light intensity 15klx, light
According to 10h/d, 70% humidity airtight greenhouse in, open bottle cap, be allowed to adapt to environment 4d, i.e.
In-bottle seeding can be completed；
2) substrate seedling exercising: the test tube Seedling of in-bottle seeding gained is taken out, cleans Rhizoma Dioscoreae esculentae with clear water
The culture medium that root system adheres to, the carbendazim solution soak time with 1000 times is 5min, after immersion
Plant in substrate seedbed, water content of substrate control be 75% mass fraction, described substrate by
Loam 2500kg, coconut palm bran (by-product after Cortex cocois radicis processing) 30kg, perlite 30kg, carbamide
200kg, potassium dihydrogen phosphate 180kg, polyacrylamide 5kg, activated carbon 3kg, anti virus herb
Extract 3kg stirring mixes, and described anti virus herb compositions is by following methods system
Standby obtain: take 10 weight portion Herba Rosmarini Officinaliss, 10 weight portion Adeps Bovis seu Bubalis, 8 weight portion Herba Pogostemoniss, 5 weight portions
Rhizoma Coptidis, 3 weight portion Radix Bupleuri, 3 weight portion Spica Prunellaes, add 10 times of weight parts waters and decoct, collect filter
Liquid also concentrates, and is dried to obtain anti virus herb extract.
Substrate seedling exercising is carried out in airtight greenhouse, covers seedbed with plastic sheeting,
Substrate seedling exercising is in the 1st～3 day totally 3 day time, and seedling exercising environmental condition is: 24 DEG C daytime temperature
Degree, 17 DEG C of nocturnal temperatures, the intensity of illumination at noon of 25klx, air humiditys of 75%.From
Within 4 days, rising, seedling exercising environmental condition is: 22 DEG C daytime temperature, 15 DEG C of nocturnal temperatures, 20klx
Noon intensity of illumination, the air humidity of 70%.From the 15th day, remove seedbed thin film.From
Within 22nd day, rising, Rhizoma Dioscoreae esculentae Seedling can transplant land for growing field crops, completes substrate seedling exercising.
Embodiment 2
The hardening off method of Rhizoma Dioscoreae esculentae group training test tube Seedling includes in-bottle seeding and two stages of substrate seedling exercising:
1) in-bottle seeding: Rhizoma Dioscoreae esculentae group training test tube Seedling is placed in temperature 25 DEG C, light intensity 17klx, light
According to 11h/d, 75% humidity airtight greenhouse in, open bottle cap, be allowed to adapt to environment 5d, i.e.
In-bottle seeding can be completed；
2) substrate seedling exercising: the test tube Seedling of in-bottle seeding gained is taken out, cleans Rhizoma Dioscoreae esculentae with clear water
The culture medium that root system adheres to, the carbendazim solution soak time with 1000 times is 6min, after immersion
Plant in substrate seedbed, water content of substrate control be 80% mass fraction, described substrate by
Loam 2800kg, coconut palm bran (by-product after Cortex cocois radicis processing) 35kg, perlite 35kg, carbamide
220kg, potassium dihydrogen phosphate 190kg, polyacrylamide 7kg, activated carbon 4kg, anti virus herb
Extract 4kg stirring mixes, and described anti virus herb compositions is by following methods system
Standby obtain: take 12 weight portion Herba Rosmarini Officinaliss, 13 weight portion Adeps Bovis seu Bubalis, 9 weight portion Herba Pogostemoniss, 8 weight portions
Rhizoma Coptidis, 4 weight portion Radix Bupleuri, 4 weight portion Spica Prunellaes, add 11 times of weight parts waters and decoct, collect filter
Liquid also concentrates, and is dried to obtain anti virus herb extract.
Substrate seedling exercising is carried out in airtight greenhouse, covers seedbed with plastic sheeting,
Substrate seedling exercising is in the 1st～3 day totally 3 day time, and seedling exercising environmental condition is: 26 DEG C daytime temperature
Degree, 18 DEG C of nocturnal temperatures, the intensity of illumination at noon of 30klx, air humiditys of 78%.From
Within 4 days, rising, seedling exercising environmental condition is: 25 DEG C daytime temperature, 17 DEG C of nocturnal temperatures, 25klx
Noon intensity of illumination, the air humidity of 75%.From the 15th day, remove seedbed thin film.From
Within 24th day, rising, Rhizoma Dioscoreae esculentae Seedling can transplant land for growing field crops, completes substrate seedling exercising.
Embodiment 3
The hardening off method of Rhizoma Dioscoreae esculentae group training test tube Seedling includes in-bottle seeding and two stages of substrate seedling exercising:
1) in-bottle seeding: Rhizoma Dioscoreae esculentae group training test tube Seedling is placed in temperature 30 DEG C, light intensity 28klx, light
According to 12h/d, 80% humidity airtight greenhouse in, open bottle cap, be allowed to adapt to environment 7d, i.e.
In-bottle seeding can be completed；
2) substrate seedling exercising: the test tube Seedling of in-bottle seeding gained is taken out, cleans Rhizoma Dioscoreae esculentae with clear water
The culture medium that root system adheres to, is 10min by the carbendazim solution soak time of 1000 times, soaks
After plant in substrate seedbed, water content of substrate control be 85% mass fraction, described substrate
By loam 3000kg, coconut palm bran (by-product after Cortex cocois radicis processing) 50kg, perlite 50kg, urine
Element 250kg, potassium dihydrogen phosphate 200kg, polyacrylamide 10kg, activated carbon 5kg, antiviral
Chinese medicine extract 5kg stirring mixes, and described anti virus herb compositions is by with lower section
Method prepares: take 15 weight portion Herba Rosmarini Officinaliss, 15 weight portion Adeps Bovis seu Bubalis, 10 weight portion Herba Pogostemoniss, 10
Weight portion Rhizoma Coptidis, 5 weight portion Radix Bupleuri, 5 weight portion Spica Prunellaes, add 12 times of weight parts waters and decoct,
Collect filtrate and concentrate, being dried to obtain anti virus herb extract.
Substrate seedling exercising is carried out in airtight greenhouse, covers seedbed with plastic sheeting,
Substrate seedling exercising is in the 1st～3 day totally 3 day time, and seedling exercising environmental condition is: 28 DEG C daytime temperature
Degree, 20 DEG C of nocturnal temperatures, the intensity of illumination at noon of 35klx, air humiditys of 80%.From
Within 4 days, rising, seedling exercising environmental condition is: 30 DEG C daytime temperature, 22 DEG C of nocturnal temperatures, 40klx
Noon intensity of illumination, the air humidity of 90%.From the 15th day, remove seedbed thin film.From
Within 25th day, rising, Rhizoma Dioscoreae esculentae Seedling can transplant land for growing field crops, completes substrate seedling exercising.
By the inventive method, Rhizoma Dioscoreae esculentae tissue cultured seedling survival rate and growth is in good condition, refers to table 1.
Table 1 Rhizoma Dioscoreae esculentae group training test tube Seedling seedling exercising effect
| Survival rate (%) | Seedling Height (cm) | The newborn number of blade | New root bar number | |
| Embodiment 1 | 89.4 | 15.8 | 3 | 4 |
| Embodiment 2 | 90.6 | 16.4 | 5 | 6 |
| Embodiment 3 | 88.9 | 15.1 | 4 | 6 |
Claims (8)
1. the hardening off method of a Rhizoma Dioscoreae esculentae group training test tube Seedling, it is characterised in that described method bag
Include following steps:
1) in-bottle seeding: Rhizoma Dioscoreae esculentae group training test tube Seedling is placed in temperature 20～30 DEG C, light intensity 15～
28klx, illumination 10～12h/d, 70～80% humidity airtight greenhouse in, open bottle cap, make
Adaptation environment 4～7d, in-bottle seeding can be completed；
2) substrate seedling exercising: by step 1) taking-up of gained test tube Seedling, clean Rhizoma Dioscoreae esculentae root system with clear water
The culture medium adhered to, plants in substrate seedbed, with plastic sheeting pair after soaking with antiseptic solution
Seedbed covers,
Described substrate by loam 2500～3000kg, coconut palm bran 30～50kg, perlite 30～
50kg, carbamide 200～250kg, potassium dihydrogen phosphate 180～200kg, polyacrylamide 5～10kg,
The stirring of activated carbon 3～5kg, anti virus herb extract 3～5kg mixes,
Described anti virus herb compositions is prepared by following methods: take 10～15 weight portions
Herba Rosmarini Officinalis, 10～15 weight portion Adeps Bovis seu Bubalis, 8～10 weight portion Herba Pogostemoniss, 5～10 weight portion Rhizoma Coptidis,
3～5 weight portion Radix Bupleuri, 3～5 weight portion Spica Prunellaes, add 10～12 times of weight parts waters decoct, and receive
Collection filtrate also concentrates, and is dried to obtain anti virus herb extract.
Hardening off method the most according to claim 1, it is characterised in that step 2) in institute
The coconut palm bran stated is the by-product after Cortex cocois radicis processing.
Hardening off method the most according to claim 1, it is characterised in that step 2) in institute
Stating antiseptic solution is the carbendazim solution of 1000 times, and described soak time is 5～10min.
Hardening off method the most according to claim 1, it is characterised in that step 2) in institute
The water content of substrate stated is 75%～85% mass fraction.
Hardening off method the most according to claim 1, it is characterised in that step 2) in institute
The substrate seedling exercising stated is to carry out in airtight greenhouse, and substrate seedling exercising is totally 3 day time at the 1st～3 day
In, seedling exercising environmental condition is: 24～28 DEG C daytime temperature, 17～20 DEG C of nocturnal temperatures, 25～
The air humidity of intensity of illumination at noon, 75%～80% of 35klx.
Hardening off method the most according to claim 1, it is characterised in that step 2) in institute
The substrate seedling exercising stated was from the 4th day, and seedling exercising environmental condition is: 22～30 DEG C daytime temperature, 15～
The air humidity of intensity of illumination at noon, 70%～90% of 22 DEG C of nocturnal temperatures, 20～40klx.
Hardening off method the most according to claim 1, it is characterised in that step 2) in institute
The substrate seedling exercising stated, from the 15th day, removes seedbed thin film.
Hardening off method the most according to claim 1, it is characterised in that step 2) in institute
The substrate seedling exercising stated was from the 22nd～25 day, and Rhizoma Dioscoreae esculentae Seedling can transplant land for growing field crops, completed substrate seedling exercising.
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| CN201610187666.1A CN105830698A (en) | 2016-03-29 | 2016-03-29 | Seedling hardening method for sweet potato tissue-cultured test-tube plantlets |
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Cited By (5)
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| CN106613966A (en) * | 2016-11-22 | 2017-05-10 | 蚌埠市涂山绿园蔬菜科研专业合作社 | Detoxification method for potato seedlings |
| CN113455319A (en) * | 2021-06-21 | 2021-10-01 | 百色学院 | Transplanting method of cassava tissue culture contaminated seedlings |
| CN113598049A (en) * | 2021-08-12 | 2021-11-05 | 福建省中科生物股份有限公司 | Chinese herbal medicine pre-cooking liquid for inhibiting tissue culture plant endophyte pollution and application method |
| CN113951073A (en) * | 2021-11-05 | 2022-01-21 | 襄阳市农业科学院 | Method for improving survival rate of sweet potato sprouts |
| CN115399215A (en) * | 2022-10-11 | 2022-11-29 | 贵州务川科华生物科技有限公司 | Preparation method of soil matrix for paper mulberry seedling hardening period |
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Application publication date: 20160810 |