CN105814199B - 修饰型脂肪酶及其用途 - Google Patents

修饰型脂肪酶及其用途 Download PDF

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Publication number
CN105814199B
CN105814199B CN201480066933.9A CN201480066933A CN105814199B CN 105814199 B CN105814199 B CN 105814199B CN 201480066933 A CN201480066933 A CN 201480066933A CN 105814199 B CN105814199 B CN 105814199B
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China
Prior art keywords
amino acid
lipase
seq
acid sequence
enzyme
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CN201480066933.9A
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English (en)
Chinese (zh)
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CN105814199A (zh
Inventor
石垣佑记
石原聪
田中俊一
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Amano Enzyme Inc
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Amano Enzyme Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING OR TREATMENT THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
    • A23C9/1203—Addition of, or treatment with, enzymes or microorganisms other than lactobacteriaceae
    • A23C9/1216—Other enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/20—Synthetic spices, flavouring agents or condiments
    • A23L27/24—Synthetic spices, flavouring agents or condiments prepared by fermentation
    • A23L27/25—Dairy flavours
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01003—Triacylglycerol lipase (3.1.1.3)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Polymers & Plastics (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Nutrition Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Dairy Products (AREA)
  • General Preparation And Processing Of Foods (AREA)
  • Seasonings (AREA)
CN201480066933.9A 2013-12-10 2014-12-08 修饰型脂肪酶及其用途 Active CN105814199B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2013255419 2013-12-10
JP2013-255419 2013-12-10
PCT/JP2014/082415 WO2015087833A1 (ja) 2013-12-10 2014-12-08 改変型リパーゼ及びその用途

Publications (2)

Publication Number Publication Date
CN105814199A CN105814199A (zh) 2016-07-27
CN105814199B true CN105814199B (zh) 2019-12-13

Family

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CN201480066933.9A Active CN105814199B (zh) 2013-12-10 2014-12-08 修饰型脂肪酶及其用途

Country Status (6)

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US (2) US10415023B2 (enExample)
EP (1) EP3081644B1 (enExample)
JP (2) JP6787668B2 (enExample)
CN (1) CN105814199B (enExample)
DK (1) DK3081644T3 (enExample)
WO (1) WO2015087833A1 (enExample)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6787668B2 (ja) * 2013-12-10 2020-11-18 天野エンザイム株式会社 改変型リパーゼ及びその用途
CN108884480A (zh) 2015-12-30 2018-11-23 帝斯曼知识产权资产管理有限公司 三酰基甘油的部分酶水解
JP2019500041A (ja) * 2015-12-30 2019-01-10 ディーエスエム アイピー アセッツ ビー．ブイ．Ｄｓｍ Ｉｐ Ａｓｓｅｔｓ Ｂ．Ｖ． トリアシルグリセロールの部分的酵素加水分解
DK3469076T3 (da) * 2016-06-10 2020-11-16 Dsm Ip Assets Bv Mutant lipase og anvendelse deraf
EP4282960B1 (en) * 2017-09-01 2025-10-01 Amano Enzyme Inc. Modified lipase and use thereof
EP3794115A2 (en) * 2018-05-18 2021-03-24 DSM IP Assets B.V. Mutant lipase and use thereof
CN112574973A (zh) * 2019-09-30 2021-03-30 东莞泛亚太生物科技有限公司 具提升耐热性的脂肪酶
US12495812B2 (en) 2020-02-28 2025-12-16 Chr. Hansen A/S Lipases, compositions, methods and uses thereof
CN118956817A (zh) * 2020-04-29 2024-11-15 上海奥博生物医药股份有限公司 脂肪酶突变体及其应用
WO2022168954A1 (ja) 2021-02-04 2022-08-11 アマノ エンザイム ユーエスエー カンパニー，リミテッド ナチュラルチーズの製造方法
WO2023031266A1 (en) 2021-09-01 2023-03-09 Chr. Hansen A/S Yarrowia lipolytica and its use for producing lipases specifically liberating short chain fatty acids

Family Cites Families (14)

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Publication number Priority date Publication date Assignee Title
DE3444282A1 (de) 1984-12-05 1986-06-05 Hoechst Ag, 6230 Frankfurt Kaesearoma
US6375947B1 (en) 1998-11-05 2002-04-23 International Flavors & Fragrances Inc. Recombinant kid pregastric esterase and methods for its production and use
EP2298873A1 (en) 1998-11-27 2011-03-23 Novozymes A/S Lipolytic enzyme variants
EP1130100A1 (en) * 2000-02-14 2001-09-05 Unilever N.V. Modified lipolytic enzymes and their use
EP1360278B1 (en) 2001-02-07 2009-09-23 Novozymes A/S Lipase variants
US7052879B2 (en) * 2001-08-31 2006-05-30 Academia Sinica Recombinant Candida rugosa lipases
JP4604524B2 (ja) * 2004-03-16 2011-01-05 味の素株式会社 変異型アルドラーゼ、並びにこれを用いた光学活性ｉｈｏｇ及び光学活性モナティンの製造方法
WO2006047469A2 (en) * 2004-10-21 2006-05-04 Novozymes, Inc. Polypeptides having lipase activity and polynucleotides encoding same
JP4915728B2 (ja) 2005-10-12 2012-04-11 花王株式会社 組換え微生物
JP5769142B2 (ja) 2007-09-04 2015-08-26 独立行政法人酒類総合研究所 ピヒア属酵母において異種タンパク質を高分泌させる方法
JP5973131B2 (ja) * 2007-09-13 2016-08-23 コデクシス， インコーポレイテッド アセトフェノンの還元のためのケトレダクターゼポリペプチド
US8637101B2 (en) 2008-02-29 2014-01-28 Dsm Ip Assets B.V. Lipases with high specificity towards short chain fatty acids and uses thereof
EP2406372B1 (en) * 2009-03-10 2017-08-23 DSM IP Assets B.V. Pregastric esterase and derivatives thereof
JP6787668B2 (ja) * 2013-12-10 2020-11-18 天野エンザイム株式会社 改変型リパーゼ及びその用途

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Altering the Substrate Specificity of Candida rugosa LIP4 by Engineering the Substrate-Binding Sites;LEE, L.-C. ET AL.;《J. AGRIC. FOOD CHEM.》;20070601;第55卷(第13期);第5103-5108页 *
Blocking the tunnel: engineering of Candida rugosa lipase mutants with short chain length specificity;SCHMITT, J. ET AL.;《PROTEIN ENG》;20020701;第15卷(第7期);第595-601页 *
Protein Engineering and Applications of Candida rugosa Lipase Isoforms;AKOH, C.C. ET AL.;《LIPIDS》;20040601;第39卷(第6期);第513-526页 *

Also Published As

Publication number Publication date
WO2015087833A1 (ja) 2015-06-18
US10415023B2 (en) 2019-09-17
US10870840B2 (en) 2020-12-22
DK3081644T3 (en) 2019-04-29
JPWO2015087833A1 (ja) 2017-03-16
CN105814199A (zh) 2016-07-27
EP3081644A1 (en) 2016-10-19
US20190345465A1 (en) 2019-11-14
JP6787668B2 (ja) 2020-11-18
JP2019193640A (ja) 2019-11-07
EP3081644A4 (en) 2017-05-24
EP3081644B1 (en) 2019-03-27
US20160319259A1 (en) 2016-11-03

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