CN105806997A - Screening method for astragalus powdery mildew prevention and treatment medicament - Google Patents
Screening method for astragalus powdery mildew prevention and treatment medicament Download PDFInfo
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- CN105806997A CN105806997A CN201410851742.5A CN201410851742A CN105806997A CN 105806997 A CN105806997 A CN 105806997A CN 201410851742 A CN201410851742 A CN 201410851742A CN 105806997 A CN105806997 A CN 105806997A
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- powdery mildew
- radix astragali
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Abstract
The invention relates to a screening method for an astragalus powdery mildew prevention and treatment medicament, and belongs to the plant protection and pesticide technical fields; mainly through comparison of sphaerotheca fuliginea preparation, determination and pharmacodynamic difference significance, a best prevention and treatment medicament is determined; compared with a traditional field test medicament screening, the method is simple to operate, short in needed time, safe and reliable, and high in accuracy rate.
Description
Technical field
The present invention relates to plant protection and technical field of pesticide, specifically the pesticide control screening technique of a kind of Radix Astragali powdery mildew.
Background technology
At the beginning of the Radix Astragali powdery mildew during morbidity leaf just, the back of the body two look unfamiliar white powder mildew, petiole, stem catch an illness also raw white mildew or mildew, and time serious, completeblade is covered by white lead, cause that blade is withered or Herb is withered.Pathogenic bacteria survives the winter on sick leaf with cleistothecium, and the next spring produces ascospore and carries out its spread in china；The mycelia survived the winter on this external bud, next year also can fall ill, and its preventing and treating is more difficult, is all use substantial amounts of pesticide control, not only makes pathogenic bacteria create Drug resistance, and the persticide residue of the Radix Astragali increases, and the quality of the Radix Astragali is greatly lowered.
Screening to Radix Astragali controlling powdery mildew medicament at present, mainly through the screening test of land for growing field crops medicament, the time used is longer, and field conditions complicated condition, the accuracy affected of result of the test, not enough for solving these, it is proposed to technical scheme.
Summary of the invention
It is an object of the invention to provide the pesticide control screening technique of a kind of Radix Astragali powdery mildew, solve the blindness use situation to chemical pesticide.
Proposing concrete scheme for solving the problems referred to above, the program includes: the preparation of Powdery Mildew, mensuration, the drug effect significance of difference compare.
One, the preparation of Powdery Mildew
Purify Powdery Mildew with conventional tissue isolation separation, make 5 × 106Individual/ml spore suspension.
Two, measure
Take the Radix Astragali blade of the consistent healthy growth of upgrowth situation, it is cut into the rectangle of 60mm × 40mm, then it is immersed in 5% olefin conversion microemulsion, 800-1000 times of liquid of 50% carbendazim, first holder 70% wettable powder, 1000 times of liquid of 25% ether phenol, 500-800 times of liquid of 70% mancozeb wettable powder, contrast with clear water, take out after 10min, the spore suspension that will prepare, is uniformly sprayed on the positive and negative of blade, and 3 repetitions are done in each process；When the lesion area that clear water processes reaches more than 90%, measure lesion area with scab analyzer.
Three, the drug effect significance of difference compares
Calculate prevention effect: prevention effect=(the average lesion area that the average lesion area of 1-chemicals treatment/clear water processes) × 100；The significance of difference with the more each medicament of spss method.
The beneficial effects of the present invention is: need not field experiment operating, method is simple, and the time used is short；Cause of disease is directly sprayed with on blade, it is not necessary to heavily cultivating pathogen, decrease operating procedure, safe and reliable, accuracy rate is high.
Detailed description of the invention
Below the implementation of the present invention being described in detail, this example is carried out under premised on technical solution of the present invention, gives detailed embodiment and concrete operating process.
Example 1
Reagent agent: 5% olefin conversion microemulsion, 800-1000 times of liquid of 50% carbendazim, first holder 70% wettable powder, 1000 times of liquid of 25% ether phenol, 500-800 times of liquid of 70% mancozeb wettable powder；
Two, the preparation of Powdery Mildew
Purify Powdery Mildew with conventional tissue isolation separation, make 5 × 106Individual/ml spore suspension.
Two, measure
Take the Radix Astragali blade of the consistent healthy growth of upgrowth situation, be cut into the rectangle of 60mm × 40mm, be then immersed in the confession reagent product that concentration ratio prepares, contrast with clear water, take out after 10min, the spore suspension that will prepare, uniformly being sprayed on the positive and negative of blade, 3 repetitions are done in each process；When the lesion area that clear water processes reaches more than 90%, measure lesion area with scab analyzer.
Four, the drug effect significance of difference compares
Calculate prevention effect: prevention effect=(the average lesion area that the average lesion area of 1-chemicals treatment/clear water processes) × 100；The significance of difference with the more each medicament of spss method.
Result shows:
| Process | Prevention effect (%) | Significant difference |
| 5% olefin conversion microemulsion | 81.67 | A |
| Sulfur. triazolone 100 accompanies liquid | 75.01 | AB |
| 50% carbendazim 800-1000 times liquid | 74.17 | AB |
| 25% 1000 times of ether phenol liquid | 67.5 | ABC |
| First holder 70% wettable powder | 66.67 | ABCD |
| 70% mancozeb wettable powder 500-800 times liquid | 65.83 | ABCD |
Claims (3)
1. the pesticide control screening technique of a Radix Astragali powdery mildew, it is characterised in that comprise the following steps: the preparation of Powdery Mildew, mensuration, the drug effect significance of difference compare.
2. the pesticide control screening technique of a kind of Radix Astragali powdery mildew according to claim 1, it is characterised in that the preparation of described Powdery Mildew refers to: purify Powdery Mildew with conventional tissue isolation separation, make 5 × 106Individual/ml spore suspension.
3. the pesticide control screening technique of a kind of Radix Astragali powdery mildew according to claim 1, it is characterized in that, described mensuration refers to: take the Radix Astragali blade of the consistent healthy growth of upgrowth situation, it is cut into the rectangle of 60mm × 40mm, then 5% olefin conversion microemulsion it is immersed in, 50% carbendazim 800-1000 times liquid, first holder 70% wettable powder, 25% 1000 times of ether phenol liquid, in 70% mancozeb wettable powder 500-800 times liquid, contrast with clear water, take out after 10min, the spore suspension that will prepare, uniformly it is sprayed on the positive and negative of blade, 3 repetitions are done in each process；When the lesion area that clear water processes reaches more than 90%, measure lesion area with scab analyzer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201410851742.5A CN105806997B (en) | 2014-12-31 | 2014-12-31 | A kind of pesticide control screening technique of Radix Astragali powdery mildew |
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| CN201410851742.5A CN105806997B (en) | 2014-12-31 | 2014-12-31 | A kind of pesticide control screening technique of Radix Astragali powdery mildew |
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| CN105806997A true CN105806997A (en) | 2016-07-27 |
| CN105806997B CN105806997B (en) | 2018-10-09 |
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| CN201410851742.5A Expired - Fee Related CN105806997B (en) | 2014-12-31 | 2014-12-31 | A kind of pesticide control screening technique of Radix Astragali powdery mildew |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106405029A (en) * | 2016-11-15 | 2017-02-15 | 融安县植保植检站 | Cumquat epiphytic chlorococcum agentia screening test system |
| CN106568903A (en) * | 2016-11-15 | 2017-04-19 | 融安县植保植检站 | Screening and testing system of drugs used for treating fortunella margarita yellowing |
| CN107064435A (en) * | 2017-05-27 | 2017-08-18 | 中国农业科学院蔬菜花卉研究所 | The mould leaf spot preventing and treating of handle of crawling and detection method and its application of preventive effect |
| CN110231441A (en) * | 2019-07-02 | 2019-09-13 | 中国农业科学院特产研究所 | A kind of Appraisal of Herbicides method |
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| CN1401247A (en) * | 2001-08-02 | 2003-03-12 | 芶普仁 | Chinese medicinal herb preparation for treating plant powdery mildew, and method for preparing same |
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| CN1401247A (en) * | 2001-08-02 | 2003-03-12 | 芶普仁 | Chinese medicinal herb preparation for treating plant powdery mildew, and method for preparing same |
| EP1900353A1 (en) * | 2006-09-15 | 2008-03-19 | L'oreal | Composition to combat localised hyperpigmentation in dark skin types |
| CN101486981A (en) * | 2009-02-10 | 2009-07-22 | 西北农林科技大学 | Bacillus for preventing wheat diseases, as well as preparation and use thereof |
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Non-Patent Citations (1)
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106405029A (en) * | 2016-11-15 | 2017-02-15 | 融安县植保植检站 | Cumquat epiphytic chlorococcum agentia screening test system |
| CN106568903A (en) * | 2016-11-15 | 2017-04-19 | 融安县植保植检站 | Screening and testing system of drugs used for treating fortunella margarita yellowing |
| CN107064435A (en) * | 2017-05-27 | 2017-08-18 | 中国农业科学院蔬菜花卉研究所 | The mould leaf spot preventing and treating of handle of crawling and detection method and its application of preventive effect |
| CN110231441A (en) * | 2019-07-02 | 2019-09-13 | 中国农业科学院特产研究所 | A kind of Appraisal of Herbicides method |
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| CN105806997B (en) | 2018-10-09 |
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Effective date of registration: 20180827 Address after: 742300 ginkgo 316 ginkgo Road, ginkgo village, Hui County, Longnan, Gansu Applicant after: Huiyuan Luyuan Technology Development Co., Ltd. Address before: 730900 3 unit 1, 1 building, 106 Lanzhou Road, Baiyin District, Baiyin, Gansu. Applicant before: Li Baizhen |
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