CN103690597B - A method of improving isoforskolin content in coleus forskohlin medicinal material - Google Patents
A method of improving isoforskolin content in coleus forskohlin medicinal material Download PDFInfo
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- CN103690597B CN103690597B CN201310636981.4A CN201310636981A CN103690597B CN 103690597 B CN103690597 B CN 103690597B CN 201310636981 A CN201310636981 A CN 201310636981A CN 103690597 B CN103690597 B CN 103690597B
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- isoforskolin
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- coleus
- forskohlin
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Abstract
The present invention relates to a kind of methods of isoforskolin content in raising coleus forskohlin medicinal material, specifically, being related to a kind of method promoting isoforskolin synthesis and accumulation using ultraviolet induction coleus forskohlin medicinal material.
Description
Technical field
The present invention relates to a kind of methods of isoforskolin content in raising coleus forskohlin medicinal material, specifically, being related to one kind
Promote the method for isoforskolin synthesis and accumulation using ultraviolet induction coleus forskohlin medicinal material.
Background technology
Coleus forskohlin derives from labiate Coleus forskohlii (Coleus forskohlii (Wild.) Briq), originates in print
The ground such as Huize, yunnan, Dongchuan of the country such as degree, Nepal and China.It is local civil for treating cough and asthma, nervous, dizziness etc.
Disease, it is significant in efficacy, it is known as " god's medicine ".Modern pharmacological studies have shown that coleus forskohlin medicinal substances extract is to bronchial asthma, congested
It is the half-day type using isoforskolin as representative that heart failure, kinds of tumors, which have apparent effect, principle active component,
Diterpene-kind compound.Furen Pharmaceutical Co., Ltd., Hubei develops " coleus forskohlin capsule " Chinese medicine by primary raw material of coleus forskohlin
New drug, Yi Huo National Drug Administration clinical research official written reply (2003L00902) and production official written reply (Z20113029), at present
Batch production is put into.To solve the problems, such as herb resource, Hubei Province Tongcheng County manually standardize base plant coleus forskohlin medicinal material.
Tongcheng County plant medicinal material compared with Yunnan Wild medicinal material, the indexs such as pharmacognosy, chemical composition and pharmacology without significant difference, but
The content of active ingredient isoforskolin is relatively low.To improve medical material quanlity, ensures quality of medicinal material and clinical application safety, improve and plant
The content for training isoforskolin in coleus forskohlin medicinal material is imperative.
Invention content
It is an object of the invention to make up the deficiencies in the prior art, isoforskolin in a kind of raising coleus forskohlin medicinal material is provided
The method of content.
To achieve the above object, the present invention adopts the following technical scheme that：
A method of improving isoforskolin content in coleus forskohlin medicinal material：It is purple including the coleus forskohlin plant after excavating
The step of outside line is irradiated.
Wherein, it includes using ultraviolet light,long wave or UV-B irradiation that the ultraviolet light, which irradiates,.Ultraviolet light,long wave is long wave ultraviolet A
Light, wavelength is between 320~400nm；Ultraviolet B is ultraviolet B radiation B light, and wavelength is between 290~320nm.Exposure intensity is 40
~160W, irradiation time 12h, during irradiation controlled at 20~30 DEG C, relative humidity be 50%~95%.
Irradiation is carried out before carrying out preliminary working to the live body coleus forskohlin plant of fresh excavation.Pre-irradiation plants coleus forskohlin
Strain first washes off silt with clear water.
The experimental results showed that using this method, compared with pre-irradiation, coleus forskohlin medicinal material isoforskolin content 20 can be improved
~90%.
The invention has the advantages that：
1. use ultraviolet induction in the present invention, can accomplish safety, controllable in the environment of closing, without other reagents and
Drug, instrument and equipment is simple, therefore less investment, at low cost；
2. implementation process is simple and practicable, isoforskolin content can be significantly improved, to people and animals' nonhazardous, to environment without shadow
It rings.
Specific implementation mode
It elaborates to technical solution provided by the invention below by specific embodiment.
Embodiment 1
The coleus forskohlin plant of fresh excavation in base is taken back into processing workshop, it is different to rinse medicinal material surface silt etc. with clear water
Object.Be placed on ultraviolet light,long wave to be irradiated, exposure intensity 40W, irradiation time 12h, during irradiation temperature be 25 DEG C, it is relatively wet
Degree is 90%.After irradiation, isoforskolin is analyzed with high performance liquid chromatography (HPLC).The result shows that coleus forskohlin after irradiation
Isoforskolin content improves 33.7%~94.4% in medicinal material root, rhizome.
Embodiment 2
It is tested in the same manner as shown in Example 1, the difference is that Ultraviolet B is irradiated, exposure intensity
For 160W, irradiation time 12h, during irradiation temperature be 24 DEG C, relative humidity 90%.Coleus forskohlin medicinal material root, root after testing
The isoforskolin content of stem improves 20%.
Embodiment 3
The instrument and illuminating method of ultraviolet light irradiation are as follows：The ultraviolet lamp tube that ultraviolet light,long wave or B will be emitted is fixed on irony
Or on wooden pillar, it is placed in about 80cm above medicinal material, powers on, open ultraviolet lamp.
Embodiment 4
The content assaying method and result of isoforskolin are as follows：The assay method of isoforskolin is carried out using HPLC methods
It measures, (the .RP-HPLC methods such as Yang Yanfang, Yang Qiaorong, Zou Guoan measure the paper that specific assay method is delivered referring to this seminar
The content Chinese medicines .27 (4) of isoforskolin in Coleus forskohlii medicinal material:260-261).This method is stable, accurate, reproducibility
It is good.
After measured, without isoforskolin in the root, rhizome and stem of the control group coleus forskohlin medicinal material of ultraviolet light irradiation
Content is respectively 0.85,0.24 and 0.035mg g-1, and after 40W UV A radiations, isoforskolin contains in three positions
Amount is increased to 1.14,0.47 and 0.199mg g-1, after 160W UV-B irradiations, the content of isoforskolin in three positions
It is increased to 1.02,0.29 and 0.049mg g-1.
Embodiment 5
Compare with the method for other raising isoforskolin contents
This method utilizes the fresh harvesting medicinal material of physics Ray UV treatment with irradiation, has the following advantages：1. effectively improving
Isoforskolin content is more than 20% to the increase rate of isoforskolin in coleus forskohlin medicinal material；2. the field cultivation that links closely is to work
The intermediate link of factory's processing, is not take up expert time；3. method simple practical, required facility is simple and easy to get, does not need specially to nurse,
Overall cost is low；4. it is environmental-friendly, and medicament residue problem is not present.
Claims (1)
1. a kind of method improving isoforskolin content in coleus forskohlin medicinal material, which is characterized in that include by the work of fresh excavation
Body coleus forskohlin plant, in the step of being irradiated with ultraviolet light before carrying out preliminary working,
Wherein, it includes using ultraviolet light,long wave or UV-B irradiation that the ultraviolet light, which irradiates,
Wherein, exposure intensity is 40 ~ 160W,
Wherein, irradiation time 12h,
Wherein, controlled at 20 ~ 30 DEG C during irradiation,
Wherein, it is 50% ~ 95% that relative humidity is controlled during irradiation.
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| CN201310636981.4A CN103690597B (en) | 2013-11-29 | 2013-11-29 | A method of improving isoforskolin content in coleus forskohlin medicinal material |
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| CN103690597A CN103690597A (en) | 2014-04-02 |
| CN103690597B true CN103690597B (en) | 2018-10-12 |
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| CN104068140B (en) * | 2014-06-20 | 2016-04-13 | 河北大学 | A kind of method improving chrysanthemum inflorescence Content of Chlorogenic Acid |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101139263A (en) * | 2007-10-12 | 2008-03-12 | 浙江省农业科学院 | Method for improving white black falsehellebore alcohol content in peanut root and stem by ultraviolet radiation |
| CN101703535A (en) * | 2009-11-05 | 2010-05-12 | 浙江大学 | Method for improving content of drug effect components of ginkgo leaf |
| CN101732434A (en) * | 2009-11-05 | 2010-06-16 | 浙江大学 | Method for inducing increase in varierty and content of secondary metabolite in clematis terniflora (medicinal plant) |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101139263A (en) * | 2007-10-12 | 2008-03-12 | 浙江省农业科学院 | Method for improving white black falsehellebore alcohol content in peanut root and stem by ultraviolet radiation |
| CN101703535A (en) * | 2009-11-05 | 2010-05-12 | 浙江大学 | Method for improving content of drug effect components of ginkgo leaf |
| CN101732434A (en) * | 2009-11-05 | 2010-06-16 | 浙江大学 | Method for inducing increase in varierty and content of secondary metabolite in clematis terniflora (medicinal plant) |
Non-Patent Citations (1)
| Title |
|---|
| 增强UV-B辐射对麻花艽叶片的抗氧化酶的影响;李惠梅等;《西北植物学报》;20051231;第25卷(第3期);519-524 * |
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