CN103690597A - Method for increasing content of isoforskolin in coleus medicine - Google Patents
Method for increasing content of isoforskolin in coleus medicine Download PDFInfo
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- CN103690597A CN103690597A CN201310636981.4A CN201310636981A CN103690597A CN 103690597 A CN103690597 A CN 103690597A CN 201310636981 A CN201310636981 A CN 201310636981A CN 103690597 A CN103690597 A CN 103690597A
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- isoforskolin
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- medical material
- colei esquirolii
- flos colei
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Abstract
The invention relates to a method for increasing the content of isoforskolin in the coleus medicine, in particular to a method for promoting isoforskolin to be synthesized and accumulated by inducing the coleus medicine by utilizing ultraviolet light.
Description
Technical field
The present invention relates to a kind of method that improves isoforskolin content in Flos Colei esquirolii medical material, specifically, relate to a kind of ultraviolet induction Flos Colei esquirolii medical material that utilizes and promote the method that isoforskolin is synthetic and accumulate.
Background technology
Flos Colei esquirolii derives from labiate Coleus forskohlii Briq. (Coleus forskohlii (Wild.) Briq), originates in the ground such as Huize, yunnan, Dongchuan of the countries such as India, Nepal and China.Local be used for the treatment of cough with asthma, the disease such as nervous, dizzy among the people, evident in efficacy, be described as " refreshing medicine ".Modern pharmacological research shows, Flos Colei esquirolii medicinal substances extract has obvious effect to bronchial asthma, congestive heart failure, kinds of tumors, and main effective ingredient is for take the labdane diterpenes compound that isoforskolin is representative.Furen Pharmaceutical Co., Ltd., Hubei take Flos Colei esquirolii and has developed " Flos Colei esquirolii capsule " new Chinese medicine as primary raw material, Yi Huo National Drug Administration clinical research official written reply (2003L00902) and production official written reply (Z20113029), dropped into batch production at present.For solving herb resource problem, base plantation Flos Colei esquirolii medical material manually standardizes in Tongcheng County, Hubei Province.Tongcheng County plantation medical material is compared with Yunnan Wild medical material, and the indexs such as the pharmacognosy, chemical composition and pharmacology are all without significant difference, but the content of effective ingredient isofloex Ke Lin is on the low side.For improving medical material quanlity, guarantee quality of medicinal material and clinical drug safety, the content that improves isoforskolin in cultivation Flos Colei esquirolii medical material is imperative.
Summary of the invention
The object of the invention is to make up the deficiencies in the prior art, a kind of method that improves isoforskolin content in Flos Colei esquirolii medical material is provided.
For achieving the above object, the present invention adopts following technical scheme:
A kind of method that improves isoforskolin content in Flos Colei esquirolii medical material: comprise the step with ultraviolet radiation by the Flos Colei esquirolii plant after excavating.
Wherein, describedly with ultraviolet radiation, comprise by ultraviolet light,long wave or UV-B irradiation.Ultraviolet light,long wave is long wave ultraviolet A light, and wavelength is between 320～400nm; UV-B is ultraviolet B radiation B light, and wavelength is between 290～320nm.
Exposure rate is 40～160W, and irradiation time is 12h, and between the light period, controlling temperature and be 20～30 ℃, relative humidity is 50%～95%.
Irradiation is the live body Flos Colei esquirolii plant of excavating fresh, before carrying out preliminary working, carries out.Pre-irradiation, first rinses Flos Colei esquirolii plant to remove silt with clear water.
Experimental result shows, adopts this method, compares with pre-irradiation, can improve Flos Colei esquirolii medical material isoforskolin content 20～90%.
Tool of the present invention has the following advantages:
1. in the present invention, adopt ultraviolet induction, under the environment of sealing, can accomplish safe, controlledly, without other reagent and medicine, instrument and equipment is simple, so less investment, and cost is low;
2. implementation process is simple, can significantly improve isoforskolin content, to people and animals' nonhazardous, on environment without impact.
The specific embodiment
Below by specific embodiment, technical scheme provided by the invention is elaborated.
Embodiment 1
Fresh Flos Colei esquirolii plant of excavating in base is taken back and processes workshop, with clear water, rinse the foreign bodies such as medical material surface silt.Be placed on ultraviolet light,long wave and irradiate, exposure rate is 40W, and irradiation time is 12h, and between the light period, temperature is that 25 ℃, relative humidity are 90%.After irradiation finishes, by high performance liquid chromatography (HPLC), analyze isoforskolin.Result shows to irradiate isoforskolin content in rear Flos Colei esquirolii medical material root, rhizome and has improved 33.7%～94.4%.
Embodiment 2
According to the method identical with embodiment 1, test, difference is that UV-B irradiates, and exposure rate is 160W, and irradiation time is 12h, and between the light period, temperature is that 24 ℃, relative humidity are 90%.The isoforskolin content of Flos Colei esquirolii medical material root, rhizome has improved 20% after testing.
Embodiment 3
Instrument and the illuminating method of ultraviolet radiation are as follows: the ultraviolet lamp tube that can launch ultraviolet light,long wave or B is fixed on irony or wooden pillar, are placed in the about 80cm in medical material top, switch on power, open uviol lamp.
Embodiment 4
Content assaying method and the result of isoforskolin are as follows: the assay method of isoforskolin adopts HPLC method to measure, paper (the Yang Yanfang that concrete assay method is delivered referring to this seminar, Yang Qiaorong, the .RP-HPLC methods such as Zou Guoan are measured the content of isoforskolin in Coleus forskohlii Briq. medical material. Chinese crude drug .27 (4): 260-261).The method is stable, accurate, favorable reproducibility.
After measured, through the content of isoforskolin in root, rhizome and the stem of the matched group Flos Colei esquirolii medical material of ultraviolet radiation, be not respectively 0.85,0.24 and 0.035mg g
-1, after 40W ultraviolet light,long wave irradiates, in three positions, the content of isoforskolin is increased to 1.14,0.47 and 0.199mg g
-1, after 160W UV-B irradiation, in three positions, the content of isoforskolin is increased to 1.02,0.29 and 0.049mg g
-1.
Embodiment 5
Improve the method comparison of isoforskolin content with other
This method utilizes physics ray ultraviolet radiation to process the fresh medical material of gathering, and has the following advantages: 1. effectively improve isoforskolin content, the increase rate of isoforskolin in Flos Colei esquirolii medical material is greater than to 20%; 2. link closely field cultivation to the intermediate link of factory process, do not take the special time; 3. method simple practical, required facility is simple and easy to get, does not need special nurse, and integrated cost is low; 4. environmental friendliness, and there is not medicament residue problem.
Claims (7)
1. a method that improves isoforskolin content in Flos Colei esquirolii medical material, is characterized in that, comprises the step with ultraviolet radiation by the Flos Colei esquirolii plant after excavating.
2. method according to claim 1, is characterized in that, wherein, describedly with ultraviolet radiation, comprises by ultraviolet light,long wave or UV-B irradiation.
3. method according to claim 1, is characterized in that, wherein, exposure rate is 40～160W.
4. method according to claim 1, is characterized in that, wherein, irradiation time is 12h.
5. method according to claim 1, is characterized in that, wherein, between the light period, controlling temperature is 20～30 ℃.
6. method according to claim 1, is characterized in that, wherein, between the light period, controlling relative humidity is 50%～95%.
7. method according to claim 1, is characterized in that, wherein, irradiation is the live body Flos Colei esquirolii plant of excavating fresh, before carrying out preliminary working, carries out.
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| CN201310636981.4A CN103690597B (en) | 2013-11-29 | 2013-11-29 | A method of improving isoforskolin content in coleus forskohlin medicinal material |
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| CN201310636981.4A CN103690597B (en) | 2013-11-29 | 2013-11-29 | A method of improving isoforskolin content in coleus forskohlin medicinal material |
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| CN103690597B CN103690597B (en) | 2018-10-12 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104068140A (en) * | 2014-06-20 | 2014-10-01 | 河北大学 | Method for increasing content of chlorogenic acid in chrysanthemum inflorescences |
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| CN101139263A (en) * | 2007-10-12 | 2008-03-12 | 浙江省农业科学院 | Method for improving white black falsehellebore alcohol content in peanut root and stem by ultraviolet radiation |
| CN101703535A (en) * | 2009-11-05 | 2010-05-12 | 浙江大学 | Method for improving content of drug effect components of ginkgo leaf |
| CN101732434A (en) * | 2009-11-05 | 2010-06-16 | 浙江大学 | Method for inducing increase in varierty and content of secondary metabolite in clematis terniflora (medicinal plant) |
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| CN101139263A (en) * | 2007-10-12 | 2008-03-12 | 浙江省农业科学院 | Method for improving white black falsehellebore alcohol content in peanut root and stem by ultraviolet radiation |
| CN101703535A (en) * | 2009-11-05 | 2010-05-12 | 浙江大学 | Method for improving content of drug effect components of ginkgo leaf |
| CN101732434A (en) * | 2009-11-05 | 2010-06-16 | 浙江大学 | Method for inducing increase in varierty and content of secondary metabolite in clematis terniflora (medicinal plant) |
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| CN104068140A (en) * | 2014-06-20 | 2014-10-01 | 河北大学 | Method for increasing content of chlorogenic acid in chrysanthemum inflorescences |
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