CN104738081A - Microcapsule for promoting Aquilaria sinensis trees or thymelaeaceae trees to produce agilawood and preparation method and application of microcapsule - Google Patents
Microcapsule for promoting Aquilaria sinensis trees or thymelaeaceae trees to produce agilawood and preparation method and application of microcapsule Download PDFInfo
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Abstract
The invention provides a microcapsule for promoting Aquilaria sinensis trees or thymelaeaceae trees to produce agilawood. The microcapsule is wrapped with agilawood inducer, and the agilawood inducer is prepared from the following components in parts by weight: 10-30 parts of plant hormone, 50-75 parts of inorganic salt and 5-15 parts of phenethyl alcohol. The invention also provides a preparation method of the microcapsule and a method for promoting agilawood formation of the Aquilaria sinensis tree or the thymelaeaceae tree. According to the method for producing agilawood, a condition similar to the natural agilawood-producing condition can be acquired, little tree damage is caused, the trees can be subjected to agilawood formation again after agilawood collection, and the yield and the quality of the formed agilawood are effectively improved.
Description
Technical field
The present invention relates to biological technical field, be specifically related to a kind of microcapsules and its preparation method and application that agallochum or suspension culture of Aquilaria sinensis tree produce agalloch eaglewood that promote.
Background technology
Agalloch eaglewood (agarwood) is Thymelaeceae (Thymelaeaceae) plant agalloch eaglewood (Aquilaria agallochaRoxb.) or suspension culture of Aquilaria sinensis [Aquilaria sinensis (Lour.) Gilg.] xylem containing black resin.The ground such as the former main product India and Malaysia, China is called import agalloch eaglewood, the provinces and regions such as the latter's main product China Hainan, Guangdong and Guangxi, is called Home-made occluder or buta-buta.Agalloch eaglewood is not only traditional rare medicinal herbs of China, Japan, India and other countries in Southeast Asia, a kind of famous and precious natural perfume material especially.Agalloch eaglewood in the Middle East, China and Japan has the history of 3000 at least, in India and France, in the Old Testament of the Holy Bible, even also has the record of agalloch eaglewood.Current agalloch eaglewood remains one of the most expensive in the world spices, and only Singapore exports the value of agalloch eaglewood every year just more than 1,200,000,000 dollars.
Healthy agalloch eaglewood or suspension culture of Aquilaria sinensis cannot generate agalloch eaglewood in normal growth situation, only after being subject to external injury, progressively can produce the wooden unit containing resin near its wound, usually will accumulate the several years could be formed, so the output capacity of agalloch eaglewood is very low under natural conditions.For this reason, people, through by scientific research and practical experience, sum up the method for the artificial Edgeworthia chrysantha such as " chopping wound method ", " half disconnected branch method ", " hole method ", " artificial inoculation's method " and " chemical method ".But these traditional artificial Edgeworthia chrysantha method complicated operations, agalloch eaglewood yield is very low, and cost is high, causes waste to a great extent.
The Chinese patent of application number 201010104119.5 discloses a kind of method that transfusion produces agalloch eaglewood on suspension culture of Aquilaria sinensis tree, and agalloch eaglewood is urged pastil and slowly instills in suspension culture of Aquilaria sinensis trunk by concrete employing transfusion, and process can obtain agalloch eaglewood after 6-12 month.The Chinese patent of application number 201310150138.5 discloses a kind of method that modified form transfusion stimulates suspension culture of Aquilaria sinensis tree to produce agilawood.Above-mentioned two kinds of methods are simple to operate, and Edgeworthia chrysantha is rapid, and the agalloch eaglewood of production all meets the content requirement of country to its ethanol soluble extractives in quality.But in Edgeworthia chrysantha process, due to the difference of agalloch eaglewood individual trees, the amount of its Edgeworthia chrysantha agent is difficult to accurate control.If Edgeworthia chrysantha agent consumption is very few, trees Edgeworthia chrysantha effect is very poor or not Edgeworthia chrysantha just, if Edgeworthia chrysantha agent consumption is excessive, will causes tree death, bring about great losses to Shannon.
Summary of the invention
For the needs in above-mentioned industry and market, the invention provides a kind of microcapsules and utilize these microcapsules to promote agallochum or suspension culture of Aquilaria sinensis tree Edgeworthia chrysantha and improve the method for the seed output and quality of agalloch eaglewood.
A kind of promotion agallochum (Aquilaria agallocha Roxb.) or suspension culture of Aquilaria sinensis tree [Aquilariasinensis (Lour.) Gilg.] produce the microcapsules of agalloch eaglewood, described encapsulation has Tambac inducer, described Tambac inducer by mass fraction be the plant hormone of 10-30 part, the benzyl carbinol of the mineral salt of 50-75 part and 5-15 part forms.
According in one embodiment of the invention, described plant hormone is one or both in ethrel, 6-benzyl aminoadenine (6-BA).
According in one embodiment of the invention, described mineral salt are one or both in sodium chloride, magnesium chloride.
According in one embodiment of the invention, described microcapsules are made up of sodium alginate and calcium chloride.
Present invention also offers the preparation method of above-mentioned microcapsules, described preparation method comprises following steps:
The sodium alginate aqueous solution being 3% by described Tambac inducer and mass fraction mixes, and then instillation and the isopyknic mass fraction of described sodium alginate aqueous solution are the calcium chloride water of 3%, stirs, is drying to obtain the microcapsules having wrapped up described Tambac inducer; Preferably, the mass ratio of described Tambac inducer and sodium alginate is 1:3.
Again on the one hand, present invention also offers a kind of method that agallochum (Aquilaria agallochaRoxb.) or suspension culture of Aquilaria sinensis tree [Aquilaria sinensis (Lour.) Gilg.] produce agalloch eaglewood that promotes, said method comprising the steps of:
1) in trunk or the one or more hole of branch mainspring cock jewel of described agallochum or suspension culture of Aquilaria sinensis tree, described microcapsules to be inserted in hole and described hole is sealed, then again inserting described microcapsules at interval of 1 ~ 2 month;
2) processing time is 3 ~ 24 months, can obtain sepia grease and yellowish-brown xylem, namely obtain agalloch eaglewood after drying by described hole.
According in one embodiment of the invention, described microcapsules are prepared by the method by comprising the following steps:
The sodium alginate aqueous solution being 3% by described Tambac inducer and mass fraction mixes, and then instillation and the isopyknic mass fraction of described sodium alginate soln are the calcium chloride water of 3%, stirs, is drying to obtain the microcapsules having wrapped up Tambac inducer; Preferably, the mass ratio of described Tambac inducer and sodium alginate is 1:3.
According in one embodiment of the invention, the diameter of a cross-section of a tree trunk 1.3 meters above the ground of described agallochum or suspension culture of Aquilaria sinensis tree is more than or equal to 8cm; Preferably, the degree of depth in described hole is more than or equal to 4cm and is less than or equal to the half of tree breast-height diameter; More preferably, described hole is positioned at apart from the trunk of ground 2 ~ 10cm.
According in one embodiment of the invention, the described processing time is 6 ~ 12 months.
According in one embodiment of the invention, the consumption of described microcapsules is every hole 10 ~ 20g.
The present invention has following beneficial effect:
The present invention is prepared into microcapsules by parcel Tambac inducer, then puts into agalloch eaglewood or suspension culture of Aquilaria sinensis trunk inside, Tambac inducer is released slowly in tree body, thus stimulates trunk to produce agalloch eaglewood.Through contrasting with modified form Edgeworthia chrysantha method (application number 201310150138.5) in the past, result shows, sequiterpene content and control methods gained agalloch eaglewood there are no significant difference in ethanol soluble extractives, linaloe oil content and linaloe oil in the agalloch eaglewood of gained of the present invention.On this basis, the agalloch eaglewood that the present invention produces is met the need of market and requirement completely, Tambac inducer microcapsules can be used on a large scale, standardization, commercial production, avoid tree death problem in agalloch eaglewood Edgeworthia chrysantha process, ensure the interests of Shannon, can large-scale promotion.The more important thing is, the method for promotion agallochum provided by the invention or suspension culture of Aquilaria sinensis tree Edgeworthia chrysantha, close to the fragrant condition of natural product, damage little to trees, after adopting perfume, trees can also carry out Edgeworthia chrysantha operation again.
Embodiment
Further illustrate the present invention below in conjunction with embodiment, should be appreciated that embodiment only for further illustrating and explaining the present invention, not for limiting the present invention.
In following embodiment, the alcohol extract of the present invention to the agalloch eaglewood random choose three increment product produced has carried out assay, same batch sample parallel test 5 times, the agalloch eaglewood sample extract content recording experiment output, all higher than 10%, reaches pharmacopoeial requirements level.After testing in the linaloe oil content that obtains of method according to the present invention and linaloe oil sequiterpene content all higher than inserting method (patent application of application number 201210197582.8) gained agalloch eaglewood according to bottle.Detection method is as follows:
Ethanol soluble extractives content detection:
The agalloch eaglewood extracted produces linaloe oil with reference to the hot dipping under the Pharmacopoeia of the People's Republic of China contained ethanol soluble extractives determination method item in 2010, and measures the ethanol soluble extractives content of gained agalloch eaglewood sample.Precision takes agalloch eaglewood medicinal powder 2.0g, puts in the conical flask of 250ml, and precision adds absolute ethyl alcohol 50ml, jam-pack, weighed weight, leaves standstill after 1 hour, connects reflux condensing tube, is heated to boiling, and keeps micro-and boil 1 hour.Let cool, take off round-bottomed flask, jam-pack, weighed weight, supply the weight of less loss with water, shake up, filter with dry filter, obtain linaloe oil.Precision measures filtrate 25ml, puts and is dried in the evaporating dish of constant weight, in water-bath after evaporate to dryness, in 105 DEG C of dryings 3 hours, move in drier, cool 30 minutes, rapid accurately weighed weight, calculates the content (%) of ethanol soluble extractives in medicinal material with dry product.
Sequiterpene content detection in linaloe oil content and linaloe oil:
Accurately take sample 10.0g, add the lixiviate three times under room temperature of 100ml ether after pulverizing respectively, after reclaiming ether, namely obtain volatile oil.
The volatile oil of sample is carried out GC-MS analysis.Instrument is the HP6890/5973GC/MS combined instrument of hewlette-packard.Chromatographic column is HP-INno-wax Polyethylene Glycol (30.0m × 320m × 0.25m) fused-silica capillary column.Temperature programming: column temperature 60 DEG C, retains 2min, is warming up to 230 DEG C with 4 DEG C/min, keeps 20min, vaporizer temperature 250 DEG C.Carrier gas is high-purity He (99.999%); Pressure 5.08psi, carrier gas flux 2.0ml/min before post; Sample size 1 μ l (acetone soln); Split ratio 10:1.
Ion gun is EI source; Ion source temperature 230 DEG C; Level Four bar temperature 150 DEG C; Electron energy 70eV; Emission current 34.6A; Multiplier voltage 1412V; Interface temperature 280 DEG C; Mass scan range is 10-550amu.Analysis result, by HPMSD chemical work, is identified in conjunction with Nist2005 standard mass spectrum picture library and WILE275 mass spectrum picture library, adopts areas of peak normalization method to calculate relative amount.
Just a kind of method utilizing microcapsules to stimulate agallochum or suspension culture of Aquilaria sinensis tree to produce agalloch eaglewood provided by the present invention is described further below.
embodiment 1the preparation of microbe microcapsules:
Mixed with 388ml distilled water by 12g sodium alginate, namely heating for dissolving obtains the sodium alginate soln of 400ml 3%.
Ethrel 0.5g, NaCl 3g, benzyl carbinol 0.5g are mixed, obtain Tambac inducer 4g, then mix with the sodium alginate of 400ml 3%, then instill the calcium chloride solution of isopyknic 3%, slowly stir, vacuum drying obtains microcapsules.
embodiment 2the preparation of Tambac inducer microcapsules
Mixed with 340ml distilled water by 10.5g sodium alginate, namely heating for dissolving obtains the sodium alginate aqueous solution of 350ml 3%.
By 6-BA 0.75g, MgCl
22.5g, benzyl carbinol 0.25g mix, and obtain Tambac inducer 3.5g, then mix with the sodium alginate of 350ml 3%, and then instill the calcium chloride water of isopyknic 3%, slowly stir, vacuum drying obtains microcapsules.
embodiment 3the preparation of Tambac inducer microcapsules
Mixed with 243ml distilled water by 7.5g sodium alginate, namely heating for dissolving obtains the sodium alginate aqueous solution of 250ml 3%.
By 6-BA 0.2g, ethrel 0.5g, MgCl
21.5g, benzyl carbinol 0.3g mix, and obtain Tambac inducer 2.5g, then mix with the sodium alginate of 250ml 3%, and then instill the calcium chloride water of isopyknic 3%, slowly stir, vacuum drying obtains microcapsules.
embodiment 4the preparation of Tambac inducer microcapsules
Mixed with 243ml distilled water by 7.5g sodium alginate, namely heating for dissolving obtains the sodium alginate aqueous solution of 250ml 3%.
By 6-BA 0.25g, ethrel 0.25g, MgCl
21.75g, benzyl carbinol 0.25g mix, and obtain Tambac inducer 2.5g, and join Edgeworthia chrysantha agent and mix with the sodium alginate of 250ml 3%, then instill the calcium chloride water of isopyknic 3%, slowly stir, vacuum drying obtains microcapsules.
embodiment 5microcapsules are stimulated production agalloch eaglewood and Experimental Comparison
1, the inventive method
Choose 5 years age of trees, the diameter of a cross-section of a tree trunk 1.3 meters above the ground is greater than the suspension culture of Aquilaria sinensis tree of 10cm, and at distance 5cm place, ground, vertical trunk uses common electric drill, and bit diameter is 0.6cm, bores the hole of 2 dark 5cm.Microcapsules 25g manufactured by embodiment 1 is filled in hole, seals hole with rubber stopper, prevent agalloch eaglewood medicament from flowing out and rain drop erosion.After 3 days, suspension culture of Aquilaria sinensis tree some leaf drops.Within every 1 month, add once, extract the sepia grease of periwound formation and filemot color changeable xylem after 3 months, after drying, obtain agalloch eaglewood.
2, control methods
The age of tree and hole the same, it, containing the aqueous solution 500ml of ethrel 0.5g, NaCl 3g, benzyl carbinol 0.5g, is slowly inputted boring, seals hole after being totally lost with rubber stopper by preparation, prevents agalloch eaglewood medicament from flowing out and rain drop erosion.After 3 days, suspension culture of Aquilaria sinensis tree some leaf drops.Within every 1 month, drip once, extract the sepia grease of periwound formation and filemot color changeable xylem after 3 months, after drying, obtain agalloch eaglewood.
3, testing inspection
Sequiterpene content detection in ethanol soluble extractives content, linaloe oil content and linaloe oil is carried out, in table 1 to agalloch eaglewood prepared by above-mentioned two kinds of methods.
The quality testing of table 1 agalloch eaglewood
| Ethanol soluble extractives content % | Linaloe oil content % | Sequiterpene content % in linaloe oil | Tree death rate % | |
| The inventive method | 12.7 | 2.1 | 17.2 | 0 |
| Control methods | 12.8 | 2.0 | 17.0 | 5.2 |
Upper table data display, the agalloch eaglewood quality of gained of the present invention and control methods are without significant difference, but tree death rate is 0, and control methods has the lethality of 5.2%.
embodiment 6microcapsules are stimulated production agalloch eaglewood and Experimental Comparison
1, the inventive method
Choose 8 years age of trees, the diameter of a cross-section of a tree trunk 1.3 meters above the ground is more than or equal to the agallochum of 8cm, and at distance 2cm place, ground, vertical trunk uses common electric drill, and bit diameter is 0.6cm, bores the hole of 2 dark 4cm.Microcapsules 20g prepared by embodiment 2 is filled in hole, seals hole with rubber stopper, prevent agalloch eaglewood medicament from flowing out and rain drop erosion.After 4 days, suspension culture of Aquilaria sinensis tree some leaf drops.Within every 2 months, add once, extract the sepia grease of periwound formation and filemot color changeable xylem after 12 months, after drying, obtain agalloch eaglewood.
2, control methods
The age of tree is the same with boring, and preparation is containing 6-BA 0.75g, MgCl
2the aqueous solution 500ml of 2.5g, benzyl carbinol 0.25g, slowly inputs boring by it, seals hole after being totally lost with rubber stopper, prevents agalloch eaglewood medicament from flowing out and rain drop erosion.After 4 days, suspension culture of Aquilaria sinensis tree some leaf drops.Within every 2 months, drip once, after 12 months, extract the sepia grease of periwound formation and filemot color changeable xylem, after drying, obtain agalloch eaglewood.
3, testing inspection
Sequiterpene content detection in ethanol soluble extractives content, linaloe oil content and linaloe oil is carried out, in table 2 to agalloch eaglewood prepared by above-mentioned two kinds of methods.
The quality testing of table 2 agalloch eaglewood
| Ethanol soluble extractives content % | Linaloe oil content % | Sequiterpene content % in linaloe oil | Tree death rate % | |
| The inventive method | 15.6 | 3.6 | 31.4 | 0 |
| Control methods | 15.8 | 3.7 | 31.5 | 4.5 |
Upper table data display, the agalloch eaglewood quality of gained of the present invention and control methods are without significant difference, but tree death rate is 0, and control methods has the lethality of 4.5%.
embodiment 7microcapsules are stimulated production agalloch eaglewood and Experimental Comparison
1, the inventive method
Choose 10 years age of trees, the diameter of a cross-section of a tree trunk 1.3 meters above the ground is more than or equal to the agallochum of 15cm, and at distance 10cm place, ground, vertical trunk uses common electric drill, and bit diameter is 0.6cm, bores the hole of 2 dark 7.5cm.Microcapsules 15g prepared by embodiment 3 is filled in hole, seals hole with rubber stopper, prevent agalloch eaglewood medicament from flowing out and rain drop erosion.After 4 days, suspension culture of Aquilaria sinensis tree some leaf drops.Within every 2 months, drip once, extract the sepia grease of periwound formation and filemot color changeable xylem after 24 months, after drying, obtain agalloch eaglewood.
2, control methods
Preparation is containing 6-BA 0.2g, ethrel 0.5g, MgCl
2the aqueous solution 500ml of 1.5g, benzyl carbinol 0.3g, slowly inputs boring by it, seals hole after being totally lost with rubber stopper, prevents agalloch eaglewood medicament from flowing out and rain drop erosion.All the other Edgeworthia chrysantha conditions are the same.After 4 days, suspension culture of Aquilaria sinensis tree some leaf drops.Within every 2 months, drip once, after 24 months, extract the sepia grease of periwound formation and filemot color changeable xylem, after drying, obtain agalloch eaglewood.
3, testing inspection
Sequiterpene content detection in ethanol soluble extractives content, linaloe oil content and linaloe oil is carried out, in table 3 to agalloch eaglewood prepared by above-mentioned two kinds of methods.
The quality testing of table 3 agalloch eaglewood
| Ethanol soluble extractives content % | Linaloe oil content % | Sequiterpene content % in linaloe oil | Tree death rate % | |
| The inventive method | 17.5 | 5.1 | 33.1 | 0 |
| Control methods | 17.3 | 5.0 | 32.8 | 6.5 |
Upper table data display, the agalloch eaglewood quality of gained of the present invention and control methods are without significant difference, but tree death rate is 0, and control methods has the lethality of 6.5%.
embodiment 8microcapsules stimulus method produces agalloch eaglewood and Experimental Comparison
1, the inventive method
Choose 10 years age of trees, the diameter of a cross-section of a tree trunk 1.3 meters above the ground is greater than the suspension culture of Aquilaria sinensis tree of 10cm, and at distance 10cm place, ground, vertical trunk uses common electric drill, and bit diameter is 0.6cm, bores the hole of 2 dark 5cm.Microcapsules 15g prepared by embodiment 4 is filled in hole, seals hole with rubber stopper, prevent agalloch eaglewood medicament from flowing out and rain drop erosion.After 4 days, suspension culture of Aquilaria sinensis tree some leaf drops.Within every 2 months, drip once, extract the sepia grease of periwound formation and filemot color changeable xylem after 12 months, after drying, obtain agalloch eaglewood.
2, control methods
Preparation is containing 6-BA 0.25g, ethrel 0.25g, MgCl
2the aqueous solution 500ml of 1.75g benzyl carbinol 0.25g, slowly inputs boring by it, seals hole after being totally lost with rubber stopper, prevents agalloch eaglewood medicament from flowing out and rain drop erosion.After 4 days, suspension culture of Aquilaria sinensis tree some leaf drops.Within every 2 months, drip once, extract the sepia grease of periwound formation and filemot color changeable xylem after 12 months, after drying, obtain agalloch eaglewood.
3, testing inspection
Sequiterpene content detection in ethanol soluble extractives content, linaloe oil content and linaloe oil is carried out, in table 4 to agalloch eaglewood prepared by above-mentioned two kinds of methods.
The quality testing of table 4 agalloch eaglewood
| Ethanol soluble extractives content % | Linaloe oil content % | Sequiterpene content % in linaloe oil | Tree death rate % | |
| The inventive method | 15.7 | 3.4 | 30.3 | 0 |
| Control methods | 15.6 | 3.5 | 30.5 | 2.5 |
Upper table data display, the agalloch eaglewood quality of gained of the present invention and control methods are without significant difference, but tree death rate is 0, and control methods has the lethality of 2.5%.
Although present invention has been description to a certain degree, significantly, under the condition not departing from the spirit and scope of the present invention, can carry out the suitable change of each condition, the preparation method of such as microcapsules should not be limited in the embodiment of the present invention method utilizing sodium alginate and calcium chloride reaction.Be appreciated that and the invention is not restricted to described embodiment, and be attributed to the scope of claim, it comprises the equivalent replacement of described each factor.
Claims (10)
1. one kind promotes that agallochum (Aquilaria agallocha Roxb.) or suspension culture of Aquilaria sinensis tree [Aquilariasinensis (Lour.) Gilg.] produce the microcapsules of agalloch eaglewood, described encapsulation has Tambac inducer, described Tambac inducer by mass fraction be the plant hormone of 10-30 part, the benzyl carbinol of the mineral salt of 50-75 part and 5-15 part forms.
2. microcapsules as claimed in claim 1, is characterized in that, described plant hormone is one or both in ethrel, 6-benzyl aminoadenine (6-BA).
3. microcapsules as claimed in claim 1 or 2, is characterized in that, described mineral salt are one or both in sodium chloride, magnesium chloride.
4. the microcapsules according to any one of claims 1 to 3, is characterized in that, described microcapsules are made up of sodium alginate and calcium chloride.
5. the preparation method of the microcapsules according to any one of Claims 1 to 4, is characterized in that, described preparation method comprises following steps:
The sodium alginate aqueous solution being 3% by described Tambac inducer and mass fraction mixes, and then instillation and the isopyknic mass fraction of described sodium alginate aqueous solution are the calcium chloride water of 3%, stirs, is drying to obtain the microcapsules having wrapped up described Tambac inducer; More preferably, the mass ratio of described Tambac inducer and sodium alginate is 1:3.
6. promote that agallochum (Aquilaria agallocha Roxb.) or suspension culture of Aquilaria sinensis tree [Aquilariasinensis (Lour.) Gilg.] produce a method for agalloch eaglewood, it is characterized in that, said method comprising the steps of:
1) described agallochum or suspension culture of Aquilaria sinensis tree trunk or the one or more hole of branch mainspring cock jewel, in hole as described in microcapsules according to any one of Claims 1 to 4 or microcapsules that as claimed in claim 5 prepared by preparation method are inserted and by as described in hole seal, then again inserted described microcapsules at interval of 1 ~ 2 month;
2) processing time is 3 ~ 24 months, can obtain sepia grease and yellowish-brown xylem, namely obtain agalloch eaglewood after drying by described hole.
7. method as claimed in claim 6, it is characterized in that, described microcapsules are prepared by the method by comprising the following steps:
The sodium alginate aqueous solution being 3% by described Tambac inducer and mass fraction mixes, and then instillation and the isopyknic mass fraction of described sodium alginate aqueous solution are the calcium chloride water of 3%, stirs, is drying to obtain the microcapsules having wrapped up Tambac inducer; Preferably, the mass ratio of described Tambac inducer and sodium alginate is 1:3.
8. method as claimed in claims 6 or 7, is characterized in that, the diameter of a cross-section of a tree trunk 1.3 meters above the ground of described agallochum or suspension culture of Aquilaria sinensis tree is more than or equal to 8cm; Preferably, the degree of depth in described hole is more than or equal to 4cm and is less than or equal to the half of tree breast-height diameter; More preferably, described hole is positioned at apart from the trunk of ground 2 ~ 10cm.
9. the method according to any one of claim 6 ~ 8, is characterized in that, the described processing time is 6 ~ 12 months.
10. the method according to any one of claim 6 ~ 9, is characterized in that, the consumption of described microcapsules is every hole 10 ~ 20g.
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| CN105165536A (en) * | 2015-09-25 | 2015-12-23 | 海南香树沉香产业股份有限公司 | Method for promoting aquilaria senensis gilg to produce agilawood and agilawood formation accelerator thereof |
| CN106007980A (en) * | 2016-05-25 | 2016-10-12 | 国森天香（北京）生物科技有限公司 | Eaglewood edgeworthia chrysantha inducer and Aquilaria agallocha production method |
| CN106069499A (en) * | 2016-06-22 | 2016-11-09 | 云南爱尼沉香技术有限公司 | A kind of method improving Rhizoma Atractylodis macrocephalae's XIANGSHU Edgeworthia chrysantha Lindl. efficiency |
| CN106465648A (en) * | 2015-08-17 | 2017-03-01 | 中国医学科学院药用植物研究所海南分所 | A kind of quick detection produces the method for Tambac inducer transporting rule and application in Lignum Aquilariae Resinatum plant body |
| CN107318903A (en) * | 2017-07-23 | 2017-11-07 | 东莞市莞香园艺科技有限公司 | A kind of Edgeworthia chrysantha derivant and a kind of agalloch eaglewood Edgeworthia chrysantha method |
| CN108307849A (en) * | 2017-01-17 | 2018-07-24 | 昱达国际生化科技有限公司 | Promote the method for agallochum secretion agalloch eaglewood resin |
| CN111373974A (en) * | 2020-05-09 | 2020-07-07 | 中山职业技术学院 | Method for inducing aquilaria sinensis to produce fragrance by using phytohormone |
| CN111849265A (en) * | 2020-06-18 | 2020-10-30 | 广州中医药大学(广州中医药研究院) | Water-absorbing controlled-release film for agilawood formation and agilawood production method |
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