CN101755629B - Liquid infusion method for producing linaloe on aquilaria sinensis trees - Google Patents
Liquid infusion method for producing linaloe on aquilaria sinensis trees Download PDFInfo
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- CN101755629B CN101755629B CN2010101041195A CN201010104119A CN101755629B CN 101755629 B CN101755629 B CN 101755629B CN 2010101041195 A CN2010101041195 A CN 2010101041195A CN 201010104119 A CN201010104119 A CN 201010104119A CN 101755629 B CN101755629 B CN 101755629B
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Abstract
The present invention belongs to biological technical field, in particular to a liquid infusion method for producing linaloe on aquilaria sinensis trees. The method includes of drilling on the aquilaria sinensis trunk, gradually dropping a linaloe catalyst into the aquilaria sinensis tree body through the liquid infusion method; the operation is repeated every 2-3 months, after 6-12 months, the chocolatebrown oil-like material and the yellowish-brown discolored eaglewood formed in the trunk is cut and is dried to obtain the linaloe; the linaloe catalyst is mixed up by a phytohormone solution and a chemical reagent solution; the chemical reagent is ferric salt or sodium salt. The process of the invention has simple technique, convenient operation, can greatly increase production quantity of the linaloe and greatly shorten the production period of the linaloe through the inducing method, has good linaloe-forming effect, can effectively ease the contradiction between supply and demand for sinensis, is applicable to large-scale standardization and commercialization production, provides a new effective path for protecting, developing and utilizing the aquilaria sinensis tree resources, and has important economical, social and ecological benefits.
Description
Technical field
The invention belongs to biological technical field, be specifically related to a kind of transfusion that utilizes and on the fragrant tree of whitewood, induce the method for producing agalloch eaglewood.
Background technology
Fragrant Aquilaria sinensis (Lour.) Gilg of whitewood, the another name buta-buta, the Thymelaeceae eaglewood, China endemic species are unique plant origins of China famous and precious aromatics medicinal material agalloch eaglewood, are listed in national secondary and lay special stress on protecting wild plant.Agalloch eaglewood is exactly China's rare Chinese medicine since ancient times, the existing history of more than one thousand years of being used as medicine.Because of it has unique aromatic odor, firmly get people and like, demand is very big always aspect stacte spices, and Hong Kong also abounds with in history and exports agalloch eaglewood because of it gains the name.
Can't generate agalloch eaglewood under the fragrant normal growth situation of whitewood, agalloch eaglewood is meant the wooden unit that contains resin that whitewood's perfume (or spice) condenses near its wound after being subjected to extraneous injury, will accumulate the several years usually could form, and natural output capacity is very low.Because the contradiction of the low output of great market demand of agalloch eaglewood and agalloch eaglewood causes the agalloch eaglewood price soaring always.Under the ordering about of agalloch eaglewood high value (the international knock-down price of present superfine LIGNUM AQUILARIAE RESINATUM MOSS is up to 10000 dollars/kilogram), the fragrant wild resource of whitewood is robbed the formula overcut, and its virgin forest destroys totally substantially.
Whitewood's perfume (or spice) in 1999 is listed in national secondary endangered protective plant and records in " Chinese Plants Red Data Book ".Whitewood's perfume (or spice) was listed in " the red register of the compromised plant of World Conservation Union " by World Conservation Union (IUCN) in 2000.Whitewood's perfume (or spice) in 2004 all is put into " CITS " (ITES, i.e. Washington pact) appendix together with the eaglewood that various countries such as originating in Southeast Asia are used to produce agalloch eaglewood.
Be protection and the rare whitewood's incense money-gifts to a temple and its monks in imminent danger of sustainable use source, the fragrant artificial planting of extensive whitewood has been carried out on ground such as Hainan, Guangdong.Whitewood's perfume (or spice) of artificial planting must apply artificial processing ability Edgeworthia chrysantha, traditional artificial Edgeworthia chrysantha method complicated operation, and the agalloch eaglewood yield is very low, and the cost height causes waste to a great extent.Therefore, how fully to improve the fragrant Edgeworthia chrysantha efficient of whitewood, the imbalance between supply and demand of alleviation agalloch eaglewood becomes the key issue in the current production.For quickening and improving the fragrant Edgeworthia chrysantha amount of whitewood, just carried out many-sided researchs such as mechanical wounding, chemistry injury and fungal infection both at home and abroad the seventies from eighties of last century, quicken the fragrant Edgeworthia chrysantha of whitewood, but Edgeworthia chrysantha efficient is all not high and unstable.
Summary of the invention
The objective of the invention is to provide a kind of and set the method for producing agalloch eaglewood at whitewood's perfume by transfusion at the deficiencies in the prior art; it is to utilize artificial induction's mode to shorten the production cycle of agalloch eaglewood effectively; improve the output of agalloch eaglewood greatly; make it to be applicable to extensive, standardization, commercial production; for protection, exploitation and the comprehensive utilization in whitewood's incense money-gifts to a temple and its monks source provides valid approach, have important economy, society and ecological benefits.
The know-why that the present invention adopts: utilize the short pastil of agalloch eaglewood to act in the fragrant tree of the whitewood body by plant hormone and chemical reagent preparation, fully contact whitewood perfume is set the xylem in the body, by setting the tissue of dredging of itself, make short pastil extend to treetop from the base portion of tree, accelerate the fragrant tree of whitewood and start defense reaction generation secondary metabolites, through accumulation, finally in the fragrant trunk of whitewood, form agalloch eaglewood, thereby reach the purpose of inducing the fragrant tree of whitewood entire body Edgeworthia chrysantha.Through inducing action variable color xylem of the sepia grease at Edgeworthia chrysantha position and yellowish-brown in the trunk after 6～12 months, after drying, obtain agalloch eaglewood.
The technical solution adopted in the present invention:
A kind of transfusion that utilizes is induced the method for producing agalloch eaglewood on the fragrant tree of whitewood, be on the high trunk of the fragrant liftoff 45～55cm of tree of whitewood, to hole, with transfusion the short pastil of agalloch eaglewood is slowly splashed in the fragrant tree of the whitewood body, seal hole then, prevent that Tambac inducer from flowing out and rain drop erosion.Applied once in 2～3 months at interval, handle after 6～12 months, extract the sepia grease and the yellowish-brown variable color xylem that form in the trunk, be agalloch eaglewood through drying.The short pastil of described agalloch eaglewood is that the chemical reagent solution with 1% plant hormone solution and 1% is that 1: 10～1: 100 ratio mixes by volume.Described chemical reagent is molysite or sodium salt.
Described plant hormone is a kind of or mixing in methyl jasmonate, ethrel or the growth hormone.
Described molysite is ferrous sulfate or frerrous chloride.
Described sodium salt is sodium acetate or sodium chloride.
The fragrant tree of described whitewood is meant to have 5～10 years age of trees, and the diameter of a cross-section of a tree trunk 1.3 meters above the ground is greater than the fragrant tree of the whitewood of 10cm.
The described degree of depth of holing on the fragrant tree of whitewood trunk is 5～8cm.
The stub that stays after the fragrant tree of whitewood saws down will grow up to the fragrant tree of the whitewood who is fit to artificial Edgeworthia chrysantha once more after 5 years.
Produce linaloe oil with reference to the hot dipping under the contained ethanol soluble extractives determination method of the Pharmacopoeia of the People's Republic of China item in 2005, and the ethanol soluble extractives content of gained agalloch eaglewood sample is measured.Precision takes by weighing agalloch eaglewood medicinal powder 2.0g, puts in the conical flask of 250ml, the accurate absolute ethyl alcohol 50ml that adds, jam-pack claims decide weight, leave standstill 1 hour after, the connection reflux condensing tube is heated to boiling, and keeps little and boiled 1 hour.Put coldly, take off round-bottomed flask, jam-pack claims decide weight, and water is supplied the weight that subtracts mistake, shakes up, and with dry filter filtration, promptly gets linaloe oil.Precision is measured filtrate 25ml, puts in the evaporating dish that is dried to constant weight, behind evaporate to dryness in the water-bath, in 105 ℃ of dryings 3 hours, moves in the drier, cools off 30 minutes, accurately rapidly claims decide weight, with the content (%) of ethanol soluble extractives in the dry product calculating medicinal material.Alcohol extract to agalloch eaglewood random choose three duplicate samples has carried out assay, same batch sample parallel test 5 times, and the agalloch eaglewood sample extract content that must test output all 〉=10% reaches pharmacopeia requirement level.The gained data list is as follows:
Agalloch eaglewood sample ethanol soluble extractives assay
| Batch | The content % of ethanol soluble extractives in the agalloch eaglewood |
| A1 | 15.15％ |
| A2 | 14.66％ |
| A3 | 13.9％ |
| A4 | 13.68％ |
| A5 | 14.03％ |
| B1 | 12.27％ |
| B2 | 13.10％ |
| B3 | 12.04％ |
| B4 | 12.55％ |
| B5 | 12.64％ |
| C1 | 13.94％ |
| C2 | 14.38％ |
| C3 | 15.69％ |
| C4 | 13.41％ |
| C5 | 14.28％ |
Technology of the present invention is simple; easy to operate; the mode that utilization is induced has greatly improved the output of agalloch eaglewood; shorten the production cycle of agalloch eaglewood; the Edgeworthia chrysantha effect is remarkable, is applicable to large-scale standardized commercialization production agalloch eaglewood, can alleviate the imbalance between supply and demand of agalloch eaglewood effectively; the protection of dialogue banksia rose resource, exploitation and the recycling valid approach that provides have important economy, society and ecological benefits.
Embodiment
The invention will be further described with non-limiting embodiment below.
Embodiment one
Choose the age of tree more than 5 years, the diameter of a cross-section of a tree trunk 1.3 meters above the ground is greater than the fragrant tree of the whitewood of 10cm, and at 47cm place, distance ground, vertical trunk uses common electric drill, and drill bit Φ=0.6cm bores the hole of dark a 7～8cm (it is good surpassing 1/2 of tree body diameter).Get the short pastil of agalloch eaglewood that 1% methyl jasmonate solution 80ml, 1% copperas solution 920ml are mixed with 1000ml, applying short pastil by the mode of boring being set the body transfusion (applies the back and seals hole with rubber stopper, prevent that Tambac inducer from flowing out and rain drop erosion), applied once in 2～3 months at interval, apply 1000ml at every turn.Dialogue banksia rose trunk was handled after 6～12 months, adopt the perusal wound thickness of tan stained wood on every side, tentatively conclude the fragrant Edgeworthia chrysantha effect of whitewood, extract the sepia grease and the filemot variable color xylem that form around the wound then, after drying, obtain agalloch eaglewood.
Embodiment two
Choose the fragrant tree of whitewood of 10 years age of trees, at 53cm place, distance ground, vertical trunk uses common electric drill, drill bit Φ=0.6cm, the hole of boring a dark 8cm.Get the short pastil of agalloch eaglewood that 1% ethephon solution 10ml, 1% growth hormone solution 5ml, 1% sodium chloride solution 985ml are mixed with 1000ml, applying short pastil by the mode of boring being set the body transfusion (applies the back and seals hole with rubber stopper, prevent that Tambac inducer from flowing out and rain drop erosion), applied once in 2～3 months at interval, apply 1000ml at every turn.Dialogue banksia rose trunk transfusion method method was handled after 6～12 months, adopt the perusal wound thickness of sepia variable color timber on every side, tentatively conclude the fragrant Edgeworthia chrysantha effect of whitewood, extract the sepia grease and the filemot variable color xylem that form around the wound then, after drying, obtain agalloch eaglewood.
Embodiment three
Choose 3 years age of trees, the fragrant tree of the whitewood about diameter of a cross-section of a tree trunk 1.3 meters above the ground 5cm, at 45cm place, distance ground, vertical trunk uses common electric drill, drill bit Φ=0.3cm, the hole of boring dark a 2～3cm.Get the short pastil of agalloch eaglewood that 1% ethephon solution 30ml, 1% growth hormone solution 10ml, 1% SAS 960ml are mixed with 1000ml, applying short pastil by the mode of boring being set the body transfusion (applies the back and seals hole with rubber stopper, prevent that Tambac inducer from flowing out and rain drop erosion), applied once in 2～3 months at interval, apply 1000ml at every turn.Dialogue banksia rose trunk transfusion method method was handled after 6～12 months, adopt the perusal wound thickness of sepia variable color timber on every side, tentatively conclude the fragrant Edgeworthia chrysantha effect of whitewood, extract the sepia grease and the filemot variable color xylem that form around the wound then, after drying, obtain agalloch eaglewood.
Adopt traditional agalloch eaglewood authentication method, all can smell strong agalloch eaglewood smell by baked wheaten cake and incense burner heating.
Claims (4)
1. a transfusion is set the method for producing agalloch eaglewood at whitewood's perfume, it is characterized in that holing on the high trunk of the fragrant liftoff 45～55cm of tree of whitewood, with transfusion agalloch eaglewood is urged pastil and slowly splashes in whitewood's perfume tree body, seals hole then; Applied once in 2～3 months at interval, handle after 6～12 months, extract the sepia grease and the yellowish-brown variable color xylem that form in the trunk, be agalloch eaglewood through drying; The short pastil of described agalloch eaglewood is that the chemical reagent solution with 1% plant hormone solution and 1% is that 1: 10～1: 100 ratio mixes by volume; Described chemical reagent is molysite or sodium salt; Described plant hormone is a kind of or mixing in methyl jasmonate, ethrel or the growth hormone; Described molysite is ferrous sulfate or frerrous chloride.
2. the transfusion that utilizes according to claim 1 is induced the method for producing agalloch eaglewood on the fragrant tree of whitewood, it is characterized in that: described sodium salt is sodium acetate or sodium chloride.
3. the transfusion that utilizes according to claim 1 is induced the method for producing agalloch eaglewood on the fragrant tree of whitewood, it is characterized in that: the fragrant tree of described whitewood is meant to have 5～10 years age of trees, and the diameter of a cross-section of a tree trunk 1.3 meters above the ground is greater than the fragrant tree of the whitewood of 10cm.
4. the transfusion that utilizes according to claim 1 is induced the method for producing agalloch eaglewood on the fragrant tree of whitewood, it is characterized in that: the described degree of depth of holing on the fragrant tree of whitewood trunk is 5～8cm.
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