CN111448932A - Method for rapidly promoting aquilaria sinensis to be fragrant - Google Patents
Method for rapidly promoting aquilaria sinensis to be fragrant Download PDFInfo
- Publication number
- CN111448932A CN111448932A CN201910060282.7A CN201910060282A CN111448932A CN 111448932 A CN111448932 A CN 111448932A CN 201910060282 A CN201910060282 A CN 201910060282A CN 111448932 A CN111448932 A CN 111448932A
- Authority
- CN
- China
- Prior art keywords
- aquilaria sinensis
- tree
- holes
- whole
- steps
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
Abstract
The invention discloses a method for rapidly promoting the entire body of aquilaria sinensis to be fragrant, and belongs to the field of wood science and technology. The method provided by the invention realizes that almost the whole tree xylem (the whole trunk and large-diameter branches) is subjected to reddish brown color change, and the oil content and the composition of the color-changed xylem reach the level of natural agilawood through detection. The method has the advantages of simple process, convenient operation, natural and pollution-free selected induction reagent, scientific and novel application method, remarkable induction effect, basically realized fragrance formation of the whole body of the aquilaria sinensis, less time consumption, capability of quickly promoting the whole body of the aquilaria sinensis to form the agilawood in a short time, capability of being used for large-scale standardized and commercialized production, capability of effectively relieving the supply-demand market conflict of the agilawood, contribution to the protection, development and utilization of aquilaria sinensis resources and important economic, social and ecological benefits.
Description
Technical Field
The invention belongs to the technical field of biology, and relates to a method for rapidly promoting aquilaria sinensis to be fragrant, in particular to a method for rapidly promoting aquilaria sinensis to be fragrant by adopting a plant growth regulator.
Background
Aquilaria sinensis (R) sinensis (L our.) Spreng.) is a tropical and subtropical evergreen arbor, is a main tree species for producing agilawood, is a rare precious medicinal plant in the world, belongs to a domestic secondary protective plant, is also an internationally protected tree material, is 5-18 meters high, dark bark gray, smooth and tough, and has wrinkles, is thinned and fluffed when young, then gradually falls off, has no hair or nearly no hair, and is sometimes absent.
The increasing demand of the market of the Chinese eaglewood at home and abroad leads the price of the Chinese eaglewood to continuously rise, and reaches the ground of 'price in grams' and 'difficult demand for one Chinese eaglewood'. In view of the pretty market situation, the plantation of aquilaria sinensis artificial forest is in a very fruitful way in recent years, but most of the aquilaria sinensis artificial forest is young forest. In addition, the formation of the fragrant substances by the aquilaria sinensis is very slow, the formation of the fragrant substances generally needs about 6 to 8 years, and the fragrance yield (namely the ratio of the fragrant substances to the whole trunk or large-diameter branches) is very low. Therefore, how to rapidly promote the formation of aquilaria sinensis edgeworthia chrysantha (agilawood) in a short period of time becomes a key technology in the efficient cultivation of aquilaria sinensis artificial forests, and the release of the supply-demand market contradiction of agilawood becomes a key problem to be solved in the current production.
At present, a small amount of methods for rapidly promoting aquilaria sinensis to knot fragrance, such as mechanical trauma, chemical reagent smearing on a trunk and the like, are available in production, but the efficiency of promoting the aquilaria sinensis to knot fragrance is generally low in the past, the effect is limited to a small range of a trunk treatment part, the yield of the obtained agilawood is low, large-scale standardized and commercial production cannot be realized, and no report is found about a method for rapidly promoting the aquilaria sinensis to knot fragrance through the whole body.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention aims to provide a method for quickly promoting the body formation of aquilaria sinensis.
The purpose of the invention is realized by the following technical scheme:
a method for rapidly promoting the body building and the fragrance formation of aquilaria sinensis comprises the following steps:
(1) selecting 3-6 years old young aquilaria sinensis trees formed by coreless wood in the vigorous transpiration period of the tree body, drilling a four-hole spiral structure at the trunk position 0.50-1.50 m away from the ground by using an electric drill, and then injecting an inducing reagent with the concentration of 0.10-1.50% into holes in a grading manner until the tree body leaves are in a severe withering state; after the injection is finished, sealing the hole by using plasticine;
(2) and (3) after 3-6 months of treatment, cutting and dissecting the tree body, wherein the xylem of the tree body is almost completely (the whole trunk and large-diameter branches) subjected to reddish brown color change, and the reddish brown xylem is agilawood, so that the purpose of generating fragrance of the whole aquilaria sinensis is achieved.
The tree body transpiration vigorous period is 5-7 months.
Preferably, the four-hole spiral structure is drilled by an electric drill at the position of a trunk 1.00m high from the ground.
The four-hole spiral structure is a structure that 4 holes with the caliber of 0.50-1.20 cm and the horizontal depth of 1/3-1/2 of a trunk are drilled obliquely downwards at an angle of 90 degrees by an electric drill at intervals of 25-35 degrees, and the vertical height distance of the 4 holes is 25.00-35.00 cm, so that the 4 holes spirally rise at the vertical height.
Preferably, the four-hole spiral structure is a structure that 4 holes with the caliber of 1.00cm and the horizontal depth of 1/2 trunk are drilled by an electric drill at an angle of 90 degrees and obliquely downwards at 35 degrees, and the vertical height distance of the 4 holes is 30.00cm, so that the 4 holes spirally rise in the vertical height.
The induction reagent is methyl jasmonate; in particular to methyl jasmonate water solution.
The condition that the leaves are heavily withered means that the leaves of the whole tree droop with little falling.
The method specifically comprises the following steps:
selecting young aquilaria sinensis trees formed by 3-6 years of raw heartwood in the transpiration exuberance period of the trees, drilling 3 holes with the diameter of 1.00cm and the horizontal depth of 1/2 at the trunk position with the height of 1.00m from the ground and at the angle of 90 degrees by using an electric drill obliquely downwards at 35 degrees, wherein the vertical height distance of 4 holes is 30.00cm, forming a four-hole spiral structure, injecting a methyl jasmonate aqueous solution with the concentration of 0.10-1.50% into the holes in a grading manner, injecting a total amount of 120.00m L into each tree, taking the condition that the leaves of the trees are severely withered as the standard, sealing the holes by using plasticine after injection, cutting and dissecting the trees after processing for 3-6 months, and obtaining agilawood as the russian odorum root, thereby achieving the purpose of the whole body and the fragrance of the aquilaria sinensis.
The method comprises the steps of taking part of reddish brown xylem, drying and crushing the xylem, accurately weighing 2.00g of heartwood sample by adopting a petroleum ether impregnation method, placing the heartwood sample into a sealed brown wide-mouthed bottle of 60.00m L, adding petroleum ether (analytically pure) with the boiling range of 60-90 ℃ of 30.00m L, placing the mixture into a constant-temperature shaking table to shake for 24 hours, repeating the treatment for 3 times, shaking and extracting for 24 hours, filtering supernatant, carrying out reduced pressure concentration in a rotary Concentrator (Concentrator5301, Eppendorf, Germany) until the petroleum ether is completely volatilized, obtaining heartwood essential oil, weighing and calculating the oil yield, measuring the components of the heartwood essential oil by adopting a gas chromatography-mass spectrometer (GC-MS) of American Agilent 6890N-5975I model, and calculating the relative content of each component of the essential oil by adopting an area normalization method.
The technical principle of the invention is as follows:
the tree is injected in 5-7 months with the largest transpiration amount, so that the inducer can diffuse in the largest range under the action of transpiration pulling force, and the range of inducing the formation of the incense is enlarged as much as possible. The four-hole spiral structure provided by the invention can increase the diffusion rate of the inducer and ensure that the inducer can be uniformly diffused to all parts of the xylem of the trunk as far as possible. The injection amount is generally enough to be injected until the leaves are seriously withered due to the difference of the sizes of the trees, and the trees can die due to the excessive amount. The selected inducer is methyl jasmonate aqueous solution, so that the methyl jasmonate released by the aqueous solution can be transmitted among xylem parenchyma cells of the trunk in a gaseous form, the induction range of the inducer is greatly expanded, and the whole tree body is formed.
Compared with the prior art, the invention has the following advantages and effects:
the method provided by the invention realizes that almost the whole tree xylem (the whole trunk and large-diameter branches) is subjected to reddish brown color change, and the oil content and the composition of the color-changed xylem reach the level of natural agilawood through detection. The method has the advantages of simple process, convenient operation, natural and pollution-free selected induction reagent, scientific and novel application method, remarkable induction effect, basically realized fragrance formation of the whole body of the aquilaria sinensis, less time consumption, capability of quickly promoting the whole body of the aquilaria sinensis to form the agilawood in a short time, capability of being used for large-scale standardized and commercialized production, capability of effectively relieving the supply-demand market conflict of the agilawood, contribution to the protection, development and utilization of aquilaria sinensis resources and important economic, social and ecological benefits.
Detailed Description
The present invention will be described in further detail with reference to examples, but the embodiments of the present invention are not limited thereto.
The test methods in the following examples, in which specific experimental conditions are not specified, are generally performed according to conventional experimental conditions or according to the experimental conditions recommended by the manufacturer. Unless otherwise specified, the reagents and materials used in the present invention are commercially available products or products obtained by a known method.
In the embodiment, the agilawood oil yield and the agilawood component type of aquilaria sinensis are measured by taking part of red brown xylem, drying and crushing the xylem, accurately weighing 2.00g of heartwood sample by adopting a petroleum ether immersion method, placing the heartwood sample into a sealed brown wide-mouth bottle with the thickness of 60.00m L, adding petroleum ether (analytically pure) with the boiling range of 60-90 ℃ of 30.00m L, placing the heartwood sample in a constant-temperature shaking table to shake for 24 hours, repeating the treatment for 3 times, shaking and extracting for 24 hours, filtering supernatant, carrying out reduced pressure concentration in a rotary Concentrator (concentator 5301, Eppendorf, Germany) until the petroleum ether is completely volatilized, obtaining agilawood essential oil, weighing and calculating the oil yield, measuring the heart wood essential oil components by adopting a gas chromatography-mass spectrometer (GC-MS) model of America Agilean Agilent 6890N-5975I, and calculating the relative content of the essential oil components by using an area.
Example 1
Selecting young trees of 5-year-old aquilaria sinensis as a material in 6 months, drilling 4 holes with the diameter of 1.00cm and the horizontal depth of about 1/2 at the position of 1.00m of a trunk of each tree at an angle of 90 degrees in an inclined downward mode by using an electric drill at an angle of 4, wherein the vertical height distance of the 4 holes is 30.00cm, so that a four-hole spiral structure is formed, injecting methyl jasmonate (MeJA) with the concentration of 0.10% into the holes in a split mode by using an injector with the diameter of 5.00m L, injecting Ethephon (ETH), Salicylic Acid (SA) and abscisic acid (ABA) with the concentration of 2.00% into the holes in a split mode to serve as a control group, injecting 100.00m L into each treated tree together, and sealing the holes by using plasticine after the injection is finished, cutting and dissecting the tree body, measuring the size of a color change range, wherein a reddish brown part is a woody sample collected after methyl jasmonate inducing reagent is used for treating for 3 months.
As can be seen from Table 1, the area of the methyljasmonate for inducing the xylem discoloration of aquilaria sinensis is significantly higher than that of other contrast group reagents, the whole body formation of agilawood is basically realized, and the oil yield and the species of the agilawood components formed by induction are also significantly higher than those of the contrast group.
TABLE 1 comparison of the effects of different treatments inducing edgeworthia chrysantha
Example 2
Injecting in 6 months as a test group, injecting in 1 month, 3 months and 9 months as a control group, selecting 5-year-old aquilaria sinensis saplings as materials, drilling 4 holes with the caliber of 1.00cm and the horizontal depth of about 1/2 of a trunk at the position of 1.00m of the trunk of each tree at an angle of 90 degrees by using an electric drill obliquely downwards at 35 degrees, wherein the vertical height distance of the 4 holes is 30.00cm, forming a four-hole spiral structure, injecting methyl jasmonate (MeJA) with the concentration of 0.10 percent into the holes by using a 5.00m L injector in a split manner, injecting each tree together by about 100.00m L, sealing the holes by using plasticine after injection, cutting and dissecting the tree body after treating for 6 months, wherein the reddish brown part is agilawood, and the result shows that the average longitudinal color-changing area of the 6-month injection is 692.60cm2While the control groups had only 282.56, 334.17 and 455.82cm of color-changing area in the longitudinal direction after 1 month, 3 months and 9 months of injection respectively2Namely, the induction effect of injection in the transpiration exuberance period is obviously higher than that of injection in other periods.
Example 3
Selecting young trees of 6-year-old aquilaria sinensis as a material, and drilling 4 apertures with 1.0 aperture at the position of 1.00m of each trunk of the trees at an angle of 90 DEG by using an electric drill for obliquely drilling downwards at 35 DEG0cm and horizontal depth of about 1/2 holes of trunk, 4 holes are spaced 30.00cm vertically to form a four-hole spiral structure, another part of the plants are injected with one hole, a single hole is used as a control group, methyl jasmonate (MeJA) with the concentration of 0.10% is injected into the hole by a 5.00m L injector in a split mode, about 100.00m L is injected into each treated tree, the leaves of the tree body are in a severe withered state, after the injection is finished, the hole is sealed by plasticine, after 6 months of treatment, the tree body is cut and dissected, and the reddish brown xylem is agilawood, the results show that the transverse and longitudinal color change areas of the injection of the four-hole spiral structure of the test group are 44.62 and 747.15cm respectively2While the control group had single-hole injection with color-changing areas of only 26.17 cm and 489.42cm in the transverse and longitudinal directions, respectively2The injection method of the four-hole spiral structure provided by the invention is scientific and novel, and the fragrance-forming effect is obviously improved.
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.
Claims (8)
1. A method for rapidly promoting the body building and the fragrance formation of aquilaria sinensis is characterized by comprising the following steps:
(1) selecting an aquilaria sinensis tree formed by 5-6 years of raw coreless wood in the transpiration vigorous period of an aquilaria sinensis tree body, drilling a four-hole spiral structure at a tree trunk position 0.50-1.50 m away from the ground by using an electric drill, and then injecting an induction reagent with the concentration of 0.10-1.50% into holes in a grading manner until the tree body leaves are in a severe withering state; after the injection is finished, sealing the hole by using plasticine;
(2) after 3-6 months of treatment, the trees are felled and dissected, the xylem of the trees is almost completely reddish brown, and the reddish brown xylem is an aquilaria sinensis fragrance-forming substance, namely commonly called agilawood, so that the aim of forming fragrance on the whole aquilaria sinensis is fulfilled.
2. The method for rapidly promoting the aquilaria sinensis to knot fragrance on the whole body according to claim 1, wherein the method comprises the following steps: the tree body transpiration vigorous period is 5-7 months.
3. The method for rapidly promoting the aquilaria sinensis to knot fragrance on the whole body according to claim 1, wherein the method comprises the following steps: at the trunk 1.00m high from the ground, a four-hole spiral structure is drilled by an electric drill.
4. The method for rapidly promoting aquilaria sinensis whole body formation according to any one of claims 1 to 3, wherein the method comprises the following steps:
the four-hole spiral structure is a structure that 4 holes with the caliber of 0.50-1.20 cm and the horizontal depth of 1/3-1/2 of a trunk are drilled obliquely downwards at an angle of 90 degrees by an electric drill at intervals of 25-35 degrees, and the vertical height distance of the 4 holes is 30.00cm, so that the 4 holes spirally rise at the vertical height.
5. The method for rapidly promoting the aquilaria sinensis to knot fragrance on the whole body according to claim 4, wherein the method comprises the following steps:
the four-hole spiral structure is a structure that 4 holes with the caliber of 1.00cm and the horizontal depth of 1/2 are drilled obliquely downwards at an angle of 90 degrees by an electric drill at intervals of 35 degrees, and the vertical height distance of the 4 holes is 30.00cm, so that the 4 holes spirally rise at the vertical height.
6. The method for rapidly promoting aquilaria sinensis whole body formation according to any one of claims 1 to 3, wherein the method comprises the following steps: the induction reagent is methyl jasmonate.
7. The method for rapidly promoting aquilaria sinensis whole body formation according to any one of claims 1 to 3, wherein the method comprises the following steps:
the condition that the leaves are heavily withered means that the leaves of the whole tree droop with little falling.
8. The method for rapidly promoting aquilaria sinensis whole body formation according to any one of claims 1 to 3, wherein the method comprises the following steps:
the fact that the xylem of the tree is almost completely reddish brown is that the whole trunk and large-diameter branches of the tree are reddish brown.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910060282.7A CN111448932A (en) | 2019-01-22 | 2019-01-22 | Method for rapidly promoting aquilaria sinensis to be fragrant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910060282.7A CN111448932A (en) | 2019-01-22 | 2019-01-22 | Method for rapidly promoting aquilaria sinensis to be fragrant |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111448932A true CN111448932A (en) | 2020-07-28 |
Family
ID=71671457
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910060282.7A Pending CN111448932A (en) | 2019-01-22 | 2019-01-22 | Method for rapidly promoting aquilaria sinensis to be fragrant |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111448932A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113079915A (en) * | 2021-03-16 | 2021-07-09 | 海南润森科技研发有限公司 | Method for circularly gathering agilawood |
| CN113273427A (en) * | 2021-06-07 | 2021-08-20 | 国森天香(海南)科技有限公司 | Agilawood rotating and forming method |
| CN114303769A (en) * | 2021-12-20 | 2022-04-12 | 海南八骏骐乡生物科技有限公司 | Method for promoting agilawood formation of aquilaria trees by girdling bark |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1541058A (en) * | 2001-05-24 | 2004-10-27 | �����մ��ѧ���»� | Cultivated agarwood |
| CN101755629A (en) * | 2010-02-01 | 2010-06-30 | 中国医学科学院药用植物研究所海南分所 | Liquid infusion method for producing linaloe on aquilaria sinensis trees |
| CN102668908A (en) * | 2012-05-31 | 2012-09-19 | 蓝均炽 | Method for producing agilawood on aquilaria plant by eccentric perfusion method and scent gland activator |
| CN102812858A (en) * | 2012-07-18 | 2012-12-12 | 中山市沉香黄农林发展有限公司 | Method for promoting formation of heartwood of aquilaria sinensis tree |
| CN105850543A (en) * | 2016-05-10 | 2016-08-17 | 中国医学科学院药用植物研究所海南分所 | Aquilaria sinensis high-pressure infusion tambac generating method and device |
| CN109006014A (en) * | 2018-08-23 | 2018-12-18 | 中国林业科学研究院热带林业研究所 | A method of rapidly promoting dalbergia odorifera entire body " Edgeworthia chrysantha " |
-
2019
- 2019-01-22 CN CN201910060282.7A patent/CN111448932A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1541058A (en) * | 2001-05-24 | 2004-10-27 | �����մ��ѧ���»� | Cultivated agarwood |
| CN101755629A (en) * | 2010-02-01 | 2010-06-30 | 中国医学科学院药用植物研究所海南分所 | Liquid infusion method for producing linaloe on aquilaria sinensis trees |
| CN102668908A (en) * | 2012-05-31 | 2012-09-19 | 蓝均炽 | Method for producing agilawood on aquilaria plant by eccentric perfusion method and scent gland activator |
| CN102812858A (en) * | 2012-07-18 | 2012-12-12 | 中山市沉香黄农林发展有限公司 | Method for promoting formation of heartwood of aquilaria sinensis tree |
| CN105850543A (en) * | 2016-05-10 | 2016-08-17 | 中国医学科学院药用植物研究所海南分所 | Aquilaria sinensis high-pressure infusion tambac generating method and device |
| CN109006014A (en) * | 2018-08-23 | 2018-12-18 | 中国林业科学研究院热带林业研究所 | A method of rapidly promoting dalbergia odorifera entire body " Edgeworthia chrysantha " |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113079915A (en) * | 2021-03-16 | 2021-07-09 | 海南润森科技研发有限公司 | Method for circularly gathering agilawood |
| CN113273427A (en) * | 2021-06-07 | 2021-08-20 | 国森天香(海南)科技有限公司 | Agilawood rotating and forming method |
| CN114303769A (en) * | 2021-12-20 | 2022-04-12 | 海南八骏骐乡生物科技有限公司 | Method for promoting agilawood formation of aquilaria trees by girdling bark |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN113016820B (en) | Inducer for promoting agaric tree to produce fragrance | |
| CN111448932A (en) | Method for rapidly promoting aquilaria sinensis to be fragrant | |
| CN109006014B (en) | Method for rapidly promoting whole-body fragrance formation of dalbergia odorifera | |
| CN103299812B (en) | Apocarya hardwood cutting seedling raising method | |
| CN103238492B (en) | Process for facilitating sprouting of adventitious roots of apocarya branches | |
| CN101663969B (en) | Method for inducing and producing dalbergia substances on dalbergia odorifera tree | |
| CN104621186B (en) | A kind of composition, the application of the method for liquid, the induction liquid on stimulation dalbergia wood Edgeworthia chrysantha is induced by its preparation | |
| CN103907472A (en) | Method for cultivating caesalpinia sappan linn in karst region | |
| CN112352580B (en) | Method for promoting aquilaria sinensis to grow fragrance under differential stress | |
| CN106962028A (en) | Camellia cuttage grafting integration quick-breeding method | |
| Chowdhury et al. | Agarwood manufacturing: a multidisciplinary opportunity for economy of Bangladesh-a review | |
| CN102499264A (en) | Agarwood promoter and method for producing agarwood in Aquilaria sinensis | |
| Sayyad et al. | Comparison of growth, nutrition and soil properties of pure and mixed stands of Populus deltoides and Alnus subcordata | |
| CN107318903A (en) | A kind of Edgeworthia chrysantha derivant and a kind of agalloch eaglewood Edgeworthia chrysantha method | |
| KR20120001111A (en) | Perfume composition containing forest and warm temperate zone species of trees and manufacturing method the same | |
| CN101913603A (en) | Method for preparing activated carbon from peeled branch wood of initiated branches in eucommia bark leaf forest | |
| CN106365751A (en) | Special chrysanthemum fertilizer | |
| CN106518367A (en) | Chrysanthemum florescence foliar fertilizer | |
| CN114874854A (en) | Extraction method of Fujian cypress leaf essential oil | |
| CN106927974A (en) | A kind of compound fertilizer for preventing and treating hickory nut dry rot and its application | |
| CN114303769A (en) | Method for promoting agilawood formation of aquilaria trees by girdling bark | |
| CN107156193A (en) | The medicine based on herbs and its preparation application method of apocarya police radicola | |
| Patil et al. | Effect of growth hormone (IBA and NAA) on rooting and sprouting behaviour of Gmelina arborea (Roxb.) | |
| CN106688788A (en) | Tea tree planting method | |
| Manetti et al. | Growth dynamics and leaf area index in chestnut coppices subjected to a new silvicultural approach: single-tree-oriented management |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20200728 |
|
| WD01 | Invention patent application deemed withdrawn after publication |