CN105802948A - 聚乙二醇定点修饰的门冬酰胺酶及其制备方法与应用 - Google Patents
聚乙二醇定点修饰的门冬酰胺酶及其制备方法与应用 Download PDFInfo
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- CN105802948A CN105802948A CN201410837456.3A CN201410837456A CN105802948A CN 105802948 A CN105802948 A CN 105802948A CN 201410837456 A CN201410837456 A CN 201410837456A CN 105802948 A CN105802948 A CN 105802948A
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- C12Y305/01001—Asparaginase (3.5.1.1)
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Abstract
Description
实验条件 | 对应实验参数 |
pH值 | 5.0 |
修饰剂 | Y-PALD-30K |
摩尔比(蛋白:PEG:还原剂) | 1:5:100 |
反应温度 | 4℃ |
反应时间(小时) | 24 |
蛋白浓度(mg/mL) | 15 |
Claims (10)
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CN201410837456.3A CN105802948B (zh) | 2014-12-29 | 2014-12-29 | 聚乙二醇定点修饰的门冬酰胺酶及其制备方法与应用 |
PCT/CN2015/072167 WO2016106941A1 (zh) | 2014-12-29 | 2015-02-03 | 聚乙二醇定点修饰的门冬酰胺酶及其制备方法与应用 |
US15/522,445 US10537620B2 (en) | 2014-12-29 | 2015-02-03 | Site-specific polyethylene glycolylated (pegylated) asparaginase, preparation method therefor and use thereof |
US16/746,264 US11103565B2 (en) | 2014-12-29 | 2020-01-17 | Site-specific polyethylene glycolylated (pegylated) asparaginase, preparation method therefor and use thereof |
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Cited By (5)
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CN107693796A (zh) * | 2016-06-22 | 2018-02-16 | 江苏众红生物工程创药研究院有限公司 | Peg定点修饰的门冬酰胺酶注射剂 |
CN108265044A (zh) * | 2016-12-31 | 2018-07-10 | 江苏众红生物工程创药研究院有限公司 | 聚乙二醇定点修饰的精氨酸脱亚胺酶及其制备方法与应用 |
CN110904085A (zh) * | 2019-12-26 | 2020-03-24 | 常州千红生化制药股份有限公司 | 一种发酵法制备门冬酰胺酶 |
CN111110857A (zh) * | 2020-03-05 | 2020-05-08 | 福建齐衡科技有限公司 | 一种长效缓释的聚乙二醇修饰抗肿瘤药物及其制备方法 |
CN111818937A (zh) * | 2017-11-30 | 2020-10-23 | 爵士制药爱尔兰有限公司 | 使用天冬酰胺酶的治疗方法 |
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CN105802948B (zh) * | 2014-12-29 | 2020-06-09 | 江苏众红生物工程创药研究院有限公司 | 聚乙二醇定点修饰的门冬酰胺酶及其制备方法与应用 |
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CN104046600A (zh) * | 2013-12-30 | 2014-09-17 | 江苏众红生物工程创药研究院有限公司 | 多臂聚乙二醇修饰剂的新用途及其在修饰门冬酰胺酶中的应用 |
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Cited By (7)
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CN107693796A (zh) * | 2016-06-22 | 2018-02-16 | 江苏众红生物工程创药研究院有限公司 | Peg定点修饰的门冬酰胺酶注射剂 |
CN107693796B (zh) * | 2016-06-22 | 2020-12-22 | 江苏众红生物工程创药研究院有限公司 | Peg定点修饰的门冬酰胺酶注射剂 |
CN108265044A (zh) * | 2016-12-31 | 2018-07-10 | 江苏众红生物工程创药研究院有限公司 | 聚乙二醇定点修饰的精氨酸脱亚胺酶及其制备方法与应用 |
CN111818937A (zh) * | 2017-11-30 | 2020-10-23 | 爵士制药爱尔兰有限公司 | 使用天冬酰胺酶的治疗方法 |
CN110904085A (zh) * | 2019-12-26 | 2020-03-24 | 常州千红生化制药股份有限公司 | 一种发酵法制备门冬酰胺酶 |
CN110904085B (zh) * | 2019-12-26 | 2021-04-16 | 常州千红生化制药股份有限公司 | 一种发酵法制备门冬酰胺酶的方法 |
CN111110857A (zh) * | 2020-03-05 | 2020-05-08 | 福建齐衡科技有限公司 | 一种长效缓释的聚乙二醇修饰抗肿瘤药物及其制备方法 |
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CN105802948B (zh) | 2020-06-09 |
US10537620B2 (en) | 2020-01-21 |
WO2016106941A1 (zh) | 2016-07-07 |
US20180161408A1 (en) | 2018-06-14 |
US11103565B2 (en) | 2021-08-31 |
US20200222515A1 (en) | 2020-07-16 |
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