CN105795480A - Extraction and analysis method and application of flavone in buckwheat - Google Patents
Extraction and analysis method and application of flavone in buckwheat Download PDFInfo
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- CN105795480A CN105795480A CN201610217023.7A CN201610217023A CN105795480A CN 105795480 A CN105795480 A CN 105795480A CN 201610217023 A CN201610217023 A CN 201610217023A CN 105795480 A CN105795480 A CN 105795480A
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- 235000009419 Fagopyrum esculentum Nutrition 0.000 title claims abstract description 95
- 229930003944 flavone Natural products 0.000 title claims abstract description 27
- 235000011949 flavones Nutrition 0.000 title claims abstract description 27
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 title claims abstract description 23
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 title claims abstract description 23
- 238000000605 extraction Methods 0.000 title claims abstract description 21
- 150000002212 flavone derivatives Chemical class 0.000 title claims abstract description 20
- 238000004458 analytical method Methods 0.000 title claims abstract description 11
- 241000219051 Fagopyrum Species 0.000 title claims abstract 39
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 27
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims abstract description 18
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 claims abstract description 15
- 235000003140 Panax quinquefolius Nutrition 0.000 claims abstract description 15
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 claims abstract description 15
- 235000008434 ginseng Nutrition 0.000 claims abstract description 15
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 claims abstract description 15
- 235000010447 xylitol Nutrition 0.000 claims abstract description 15
- 239000000811 xylitol Substances 0.000 claims abstract description 15
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 claims abstract description 15
- 229960002675 xylitol Drugs 0.000 claims abstract description 15
- 239000000203 mixture Substances 0.000 claims abstract description 14
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 11
- 235000021395 porridge Nutrition 0.000 claims abstract description 9
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- 235000013312 flour Nutrition 0.000 claims description 29
- 238000002156 mixing Methods 0.000 claims description 20
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 18
- 229930003935 flavonoid Natural products 0.000 claims description 17
- 150000002215 flavonoids Chemical class 0.000 claims description 17
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- 239000000463 material Substances 0.000 claims description 15
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- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Chemical compound [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 claims description 12
- LPXPTNMVRIOKMN-UHFFFAOYSA-M sodium nitrite Chemical compound [Na+].[O-]N=O LPXPTNMVRIOKMN-UHFFFAOYSA-M 0.000 claims description 12
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- 239000000843 powder Substances 0.000 claims description 7
- BNGXYYYYKUGPPF-UHFFFAOYSA-M (3-methylphenyl)methyl-triphenylphosphanium;chloride Chemical compound [Cl-].CC1=CC=CC(C[P+](C=2C=CC=CC=2)(C=2C=CC=CC=2)C=2C=CC=CC=2)=C1 BNGXYYYYKUGPPF-UHFFFAOYSA-M 0.000 claims description 6
- PHOQVHQSTUBQQK-SQOUGZDYSA-N D-glucono-1,5-lactone Chemical compound OC[C@H]1OC(=O)[C@H](O)[C@@H](O)[C@@H]1O PHOQVHQSTUBQQK-SQOUGZDYSA-N 0.000 claims description 6
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 6
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims description 6
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims description 6
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- 239000003153 chemical reaction reagent Substances 0.000 claims description 6
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- 235000012209 glucono delta-lactone Nutrition 0.000 claims description 6
- 239000000182 glucono-delta-lactone Substances 0.000 claims description 6
- 229960003681 gluconolactone Drugs 0.000 claims description 6
- 229910000027 potassium carbonate Inorganic materials 0.000 claims description 6
- 239000002244 precipitate Substances 0.000 claims description 6
- 235000010288 sodium nitrite Nutrition 0.000 claims description 6
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- 239000012086 standard solution Substances 0.000 claims description 6
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- 239000003795 chemical substances by application Substances 0.000 claims description 5
- IKGXIBQEEMLURG-BKUODXTLSA-N rutin Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@@H]1OC[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 IKGXIBQEEMLURG-BKUODXTLSA-N 0.000 claims description 5
- 229960004555 rutoside Drugs 0.000 claims description 5
- 239000001488 sodium phosphate Substances 0.000 claims description 5
- 229910000162 sodium phosphate Inorganic materials 0.000 claims description 5
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 claims description 5
- JMGZEFIQIZZSBH-UHFFFAOYSA-N Bioquercetin Natural products CC1OC(OCC(O)C2OC(OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5)C(O)C2O)C(O)C(O)C1O JMGZEFIQIZZSBH-UHFFFAOYSA-N 0.000 claims description 4
- IVTMALDHFAHOGL-UHFFFAOYSA-N eriodictyol 7-O-rutinoside Natural products OC1C(O)C(O)C(C)OC1OCC1C(O)C(O)C(O)C(OC=2C=C3C(C(C(O)=C(O3)C=3C=C(O)C(O)=CC=3)=O)=C(O)C=2)O1 IVTMALDHFAHOGL-UHFFFAOYSA-N 0.000 claims description 4
- FDRQPMVGJOQVTL-UHFFFAOYSA-N quercetin rutinoside Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 FDRQPMVGJOQVTL-UHFFFAOYSA-N 0.000 claims description 4
- 230000001105 regulatory effect Effects 0.000 claims description 4
- ALABRVAAKCSLSC-UHFFFAOYSA-N rutin Natural products CC1OC(OCC2OC(O)C(O)C(O)C2O)C(O)C(O)C1OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5 ALABRVAAKCSLSC-UHFFFAOYSA-N 0.000 claims description 4
- 235000005493 rutin Nutrition 0.000 claims description 4
- 230000001954 sterilising effect Effects 0.000 claims description 4
- 241001619461 Poria <basidiomycete fungus> Species 0.000 claims description 3
- 244000007021 Prunus avium Species 0.000 claims description 3
- 235000010401 Prunus avium Nutrition 0.000 claims description 3
- UIIMBOGNXHQVGW-DEQYMQKBSA-M Sodium bicarbonate-14C Chemical compound [Na+].O[14C]([O-])=O UIIMBOGNXHQVGW-DEQYMQKBSA-M 0.000 claims description 3
- 229930006000 Sucrose Natural products 0.000 claims description 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 3
- 102000011759 adducin Human genes 0.000 claims description 3
- 108010076723 adducin Proteins 0.000 claims description 3
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- 238000000576 coating method Methods 0.000 claims description 3
- 229940109275 cyclamate Drugs 0.000 claims description 3
- HCAJEUSONLESMK-UHFFFAOYSA-N cyclohexylsulfamic acid Chemical compound OS(=O)(=O)NC1CCCCC1 HCAJEUSONLESMK-UHFFFAOYSA-N 0.000 claims description 3
- 239000012535 impurity Substances 0.000 claims description 3
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- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims description 3
- 235000017557 sodium bicarbonate Nutrition 0.000 claims description 3
- 229910000029 sodium carbonate Inorganic materials 0.000 claims description 3
- FQENQNTWSFEDLI-UHFFFAOYSA-J sodium diphosphate Chemical group [Na+].[Na+].[Na+].[Na+].[O-]P([O-])(=O)OP([O-])([O-])=O FQENQNTWSFEDLI-UHFFFAOYSA-J 0.000 claims description 3
- PPASLZSBLFJQEF-RKJRWTFHSA-M sodium erythorbate Chemical compound [Na+].OC[C@@H](O)[C@H]1OC(=O)C(O)=C1[O-] PPASLZSBLFJQEF-RKJRWTFHSA-M 0.000 claims description 3
- 229940048086 sodium pyrophosphate Drugs 0.000 claims description 3
- 235000019832 sodium triphosphate Nutrition 0.000 claims description 3
- 230000003068 static effect Effects 0.000 claims description 3
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- 239000000126 substance Substances 0.000 claims description 3
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- 235000019818 tetrasodium diphosphate Nutrition 0.000 claims description 3
- 239000001577 tetrasodium phosphonato phosphate Substances 0.000 claims description 3
- 230000001476 alcoholic effect Effects 0.000 claims description 2
- 238000004090 dissolution Methods 0.000 claims description 2
- 238000010298 pulverizing process Methods 0.000 claims description 2
- 238000007873 sieving Methods 0.000 claims description 2
- 238000002791 soaking Methods 0.000 claims description 2
- 235000013305 food Nutrition 0.000 abstract description 10
- 239000010903 husk Substances 0.000 abstract description 6
- 210000004204 blood vessel Anatomy 0.000 abstract description 4
- 230000003205 diastolic effect Effects 0.000 abstract description 4
- 230000004089 microcirculation Effects 0.000 abstract description 4
- 229940089161 ginsenoside Drugs 0.000 abstract description 3
- 229930182494 ginsenoside Natural products 0.000 abstract description 3
- 238000004519 manufacturing process Methods 0.000 abstract 3
- 244000241872 Lycium chinense Species 0.000 abstract 1
- 235000015468 Lycium chinense Nutrition 0.000 abstract 1
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- 206010012601 diabetes mellitus Diseases 0.000 description 9
- 239000008280 blood Substances 0.000 description 5
- 210000004369 blood Anatomy 0.000 description 5
- REFJWTPEDVJJIY-UHFFFAOYSA-N Quercetin Chemical compound C=1C(O)=CC(O)=C(C(C=2O)=O)C=1OC=2C1=CC=C(O)C(O)=C1 REFJWTPEDVJJIY-UHFFFAOYSA-N 0.000 description 4
- 230000002526 effect on cardiovascular system Effects 0.000 description 4
- 150000002213 flavones Chemical class 0.000 description 4
- 208000024172 Cardiovascular disease Diseases 0.000 description 3
- 241000209140 Triticum Species 0.000 description 3
- 235000021307 Triticum Nutrition 0.000 description 3
- 230000008602 contraction Effects 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- -1 flavone compound Chemical class 0.000 description 3
- 230000036541 health Effects 0.000 description 3
- 210000002464 muscle smooth vascular Anatomy 0.000 description 3
- NIFIFKQPDTWWGU-UHFFFAOYSA-N pyrite Chemical compound [Fe+2].[S-][S-] NIFIFKQPDTWWGU-UHFFFAOYSA-N 0.000 description 3
- 229910052683 pyrite Inorganic materials 0.000 description 3
- 239000011028 pyrite Substances 0.000 description 3
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 2
- YXOLAZRVSSWPPT-UHFFFAOYSA-N Morin Chemical compound OC1=CC(O)=CC=C1C1=C(O)C(=O)C2=C(O)C=C(O)C=C2O1 YXOLAZRVSSWPPT-UHFFFAOYSA-N 0.000 description 2
- ZVOLCUVKHLEPEV-UHFFFAOYSA-N Quercetagetin Natural products C1=C(O)C(O)=CC=C1C1=C(O)C(=O)C2=C(O)C(O)=C(O)C=C2O1 ZVOLCUVKHLEPEV-UHFFFAOYSA-N 0.000 description 2
- HWTZYBCRDDUBJY-UHFFFAOYSA-N Rhynchosin Natural products C1=C(O)C(O)=CC=C1C1=C(O)C(=O)C2=CC(O)=C(O)C=C2O1 HWTZYBCRDDUBJY-UHFFFAOYSA-N 0.000 description 2
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- MWDZOUNAPSSOEL-UHFFFAOYSA-N kaempferol Natural products OC1=C(C(=O)c2cc(O)cc(O)c2O1)c3ccc(O)cc3 MWDZOUNAPSSOEL-UHFFFAOYSA-N 0.000 description 2
- 239000011777 magnesium Substances 0.000 description 2
- 229910052749 magnesium Inorganic materials 0.000 description 2
- UXOUKMQIEVGVLY-UHFFFAOYSA-N morin Natural products OC1=CC(O)=CC(C2=C(C(=O)C3=C(O)C=C(O)C=C3O2)O)=C1 UXOUKMQIEVGVLY-UHFFFAOYSA-N 0.000 description 2
- 235000007708 morin Nutrition 0.000 description 2
- YNPNZTXNASCQKK-UHFFFAOYSA-N phenanthrene Chemical compound C1=CC=C2C3=CC=CC=C3C=CC2=C1 YNPNZTXNASCQKK-UHFFFAOYSA-N 0.000 description 2
- 235000005875 quercetin Nutrition 0.000 description 2
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- 239000002994 raw material Substances 0.000 description 2
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- 241000196324 Embryophyta Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 206010018473 Glycosuria Diseases 0.000 description 1
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- XETQTCAMTVHYPO-UHFFFAOYSA-N Isocamphan von ungewisser Konfiguration Natural products C1CC2C(C)(C)C(C)C1C2 XETQTCAMTVHYPO-UHFFFAOYSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
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- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 241000219050 Polygonaceae Species 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
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- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229940093797 bioflavonoids Drugs 0.000 description 1
- 230000036770 blood supply Effects 0.000 description 1
- 229930006742 bornane Natural products 0.000 description 1
- 230000023852 carbohydrate metabolic process Effects 0.000 description 1
- 235000021256 carbohydrate metabolism Nutrition 0.000 description 1
- 208000013527 cardiovascular neoplasm Diseases 0.000 description 1
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- 238000013461 design Methods 0.000 description 1
- 238000012631 diagnostic technique Methods 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
-
- A—HUMAN NECESSITIES
- A21—BAKING; EDIBLE DOUGHS
- A21D—TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
- A21D2/00—Treatment of flour or dough by adding materials thereto before or during baking
- A21D2/08—Treatment of flour or dough by adding materials thereto before or during baking by adding organic substances
- A21D2/36—Vegetable material
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Physics & Mathematics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Nutrition Science (AREA)
- Polymers & Plastics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses an extraction and analysis method and an application of flavone in buckwheat and belongs to the technical field of food engineering. The application comprises production of a buckwheat drink, production of ginseng and buckwheat biscuits and production of eight-treasure buckwheat porridge. Buckwheat is mashed, and buckwheat kernels and buckwheat husks are separated; 20 g of the buckwheat, the buckwheat kernels and the buckwheat husks are taken respectively and filtered by a 60-mesh sieve after being crushed; the sieved buckwheat, the buckwheat kernels and the buckwheat husks are placed in a conical flask, water with the amount 6 times the buckwheat, the buckwheat kernels and the buckwheat husks is added to the conical flask, decoction is performed twice, 2 hours each time, water decoctions are mixed, 95% ethanol is added, the concentration is adjusted to 70%, the mixture is left to stand for 24 hours and then filtered, filtrate is collected, the volume is metered to 2000 ml, and a test solution is obtained. After the flavone in the buckwheat kernels and the buckwheat husks is extracted, the flavone can soften blood vessels, improve microcirculation, improve systolic and diastolic functions of body blood vessel smooth muscle after ginsenoside, xylitol, Chinese wolfberry essence, citric acid and the like are added.
Description
Technical field
The invention belongs to Food Engineering Development field, especially relate to extraction and the application of a kind of buckwheat flavonoids.
Background technology
Diabetes are a kind of owing to internal insulin secretion is relative or the systemic disease that carbohydrate metabolism disturbance is principal character that is definitely not enough and that cause.The epidemiologic data of 1980 shows, the prevalence of China's diabetes is about 0.67%, and by 2008, through investigation and analysis, estimation China reached 9.7% more than 20 years old adult's diabetes prevalence.Based on the population base of China 1,300,000,000, add the prevalence that diabetes are so high, short 30 years, China or become diabetes the first big country in the world.Progress along with growth in the living standard, aged tendency of population and living-pattern preservation and diagnostic techniques, the sickness rate of diabetes is rising year by year, expect 2025 and reach 300,000,000 people, become the third-largest non-infective disease after cardiovascular and malignant tumor, serious harm human health and social development.It is the positive serious threat of the third-largest Health Killer after malignant tumor, the cardiovascular disease health of that China is national that diabetes are known as.
Raising day by day along with living standard, Cereals group food is not limited only to the single foods such as Semen Tritici aestivi and Oryza sativa L. by people, and to it is considered that be that the food of coarse grain has had new understanding, and its nutritive value and food therapy function be there has also been new research, and reached higher level.
Semen Fagopyri Esculenti (buckwheat) has another name called crow wheat, flower wheat or triangle wheat, it is that Polygonaceae (polygon-naeae), Fagopyrum (Fagopyrum) be dicotyledonous annual or perennial root plant, having the multiple pharmacologically actives such as antioxidation, blood sugar lowering, blood fat reducing, antitumor, its main active substances is flavone compound.
One investigation of Public Health College, Harbin Medical Univ shows, the crowd in staple food Semen Fagopyri Esculenti area, its blood sugar level and diabetes prevalence are significantly lower than the crowd in not edible buckwheat area.Wherein, the recall rate of resident's elevated blood glucose levels (namely higher than the blood sugar level of normal value) of staple food Semen Fagopyri Esculenti is 1.6%；But not the crowd of staple food Semen Fagopyri Esculenti, the recall rate of hyperglycemia is 7.33%, and the latter is higher than the former nearly 4 times.The diabetes prevalence in staple food Semen Fagopyri Esculenti and not edible buckwheat area also differs about 1 times, and the former is 1.88%, and the latter is 3.84%.This conclusion is pointed out, and Semen Fagopyri Esculenti is as effectively reducing the pathogenetic rain fed crops of glycosuria, it should occur in more on the dining table of common people.
The principle active component of buckwheat flavonoids treatment cardiovascular and cerebrovascular disease is globulariacitrin, morin, Quercetin, camphane phenanthrene alcohol.Other various compositions associate not quite with the treatment of cardiovascular and cerebrovascular disease.In Semen Fagopyri Esculenti rich in bioflavonoids main component be rutin, have another name called VP, its primary efficacy is vessel softening, improves microcirculation, and Quercetin therein and morin etc. can improve contraction and the diastolic function of body vascular smooth muscle；Cardiac activity is had well-tuned effect by magnesium, cardiac rhythm and the heart can be made to put forth energy conduction and slow down, and increases blood supply of cardiac muscle amount, favourable diastole and rest.Some trace element (such as magnesium, ferrum, copper, potassium etc.) contained in Fagopyrum esculentum Moench powder have protective effect for cardiovascular.
Therefore, from Semen Fagopyri Esculenti, extract flavone compound, as the raw material of health food or medicine, there is better prospect.At present, it is many with buckwheat seed and husk of Radix Et Rhizoma Fagopyri Tatarici for raw material to extract the research of flavone compound from Semen Fagopyri Esculenti, and the flavones content in Buckwheat shell is higher than buckwheat seed, and Buckwheat shell belongs to refuse reclamation.Therefore need badly in the middle of prior art and want a kind of novel technical scheme to solve this problem.
Summary of the invention
The technical problem to be solved is: provide extraction and analytical method and the application thereof of pyrite in a kind of Semen Fagopyri Esculenti, after the extracting flavonoids in shelled buckwheat, Testa Fagopyri Esculenti, add the compositions such as ginsenoside, xylitol, Fructus Lycii essence, citric acid, blood vessel can be changed, improve microcirculation, improve contraction and the diastolic function of body vascular smooth muscle.
The extraction and analytical method of flavone in a kind of Semen Fagopyri Esculenti, is characterized in that: comprise the following steps,
Step one, flavone extraction
Take Semen Fagopyri Esculenti to smash to pieces, separate shelled buckwheat, Testa Fagopyri Esculenti；Take Semen Fagopyri Esculenti 20g, shelled buckwheat 20g, Testa Fagopyri Esculenti 20g respectively, after pulverizing, cross 60 mesh sieves；Semen Fagopyri Esculenti after sieving, shelled buckwheat, Testa Fagopyri Esculenti are respectively placed in conical flask, in conical flask, add 6 times amount water, decoct 2 times, each 2 hours, merge decocting liquid, add 95% ethanol, concentration is regulated to 70%, filter after standing 24 hours, collect filtrate, be settled to 2000ml and obtain need testing solution；
Step 2, prepare standard curve
Weigh the rutin standard substance 6.0mg of 120 DEG C of dry constant weights, add the alcoholic solution that concentration is 70% and dissolve, be settled in 50ml volumetric flask and mix, make the standard solution that concentration is 0.12mg/ml；Take standard solution 1.0ml, 2.0ml, 3.0ml, 4.0ml, 5.0ml respectively and be placed in 25ml volumetric flask, add water to 5ml respectively, after dosing the sodium nitrite 1.0ml mixing that concentration is 5%, stand 6min；6min is stood after being separately added into the aluminum nitrate solution 1.0ml mixing of 10%；L5min, parallel reagent blank is stood after being separately added into the sodium hydroxide solution 10ml mixing of 4%；It is respectively placed in 510nm wavelength place, measures absorbance；
Step 3, sample size measure
Take Semen Fagopyri Esculenti need testing solution 3ml, shelled buckwheat need testing solution 3ml, Testa Fagopyri Esculenti need testing solution 1ml that described step one obtains, be placed in 25ml volumetric flask, add water to 5ml respectively, after adding 5% sodium nitrite 1.0ml mixing, place 6min；6min is stood after adding the aluminum nitrate 1.0ml mixing that concentration is 10%；L5min, parallel reagent blank is stood after adding the sodium hydroxide solution l0ml mixing that concentration is 4%；It is respectively placed in 510nm wavelength place, measures absorbance.
The application of flavone in a kind of Semen Fagopyri Esculenti, is characterized in that: include the making of the making of buckwheat beverage, the making of Radix Ginseng Semen Fagopyri Esculenti cookies and eight-ingredient buckwheat porridge；
The making of buckwheat beverage
Step one, taking sweet cherry roots skin, add the aqueous sodium carbonate of the PH=9 of 10～20 times amount, carry out supersound extraction 3 times, each extraction time is 10min～50min, takes supernatant after centrifugal；
Step 2, the centrifugal liquid that described step one obtains being concentrated into the 1/2 of original volume, the hydrochloric acid with 10% regulates to PH=4, by the concentrated solution after regulating, static placement 12h～48h, taking precipitate after centrifugal；
Step 3, take the precipitate that described step 2 obtains, be put in drying under reduced pressure case, when 40 DEG C～60 DEG C of 10Pa～30Pa, dry 8h～12h, it is thus achieved that buckwheat flavonoids；
Step 4, take described step 3 obtain buckwheat flavonoids 50mg～80mg, add 0.1mol/L solution of potassium carbonate, after carrying out ultrasonic dissolution 10min～30min, it is thus achieved that buckwheat flavonoids is completely dissolved liquid；
Step 5, take in described step 4 obtain buckwheat flavonoids be completely dissolved liquid, Radix Ginseng 3g～6g, separately taking xylitol 5g～15g, potassium carbonate 5mg～10mg, citric acid 0.05g～0.2g, Fructus Lycii essence 0.5ml～1.5ml, cyclamate 0.1g～0.4g, after adding pure water 1000ml, mixing obtains mixed liquor；
Step 6, the mixed solution described step 5 obtained, carry out membrane filtration, sterilizing through 150 μm of semipermeable membranes, obtain buckwheat beverage；
The making of Radix Ginseng Semen Fagopyri Esculenti cookies
In step one, cookies, each formula proportion is: Buckwheat flour 15%～40%, light flour 30%～60%, soybean oil 8%～12%, baking powder 2%～4%, sodium bicarbonate 1%～2%, glucono-δ-lactone 1%～3%, Sal 0.1%～0.4%, xylitol 10%～16%, water 25%～30%, Radix Ginseng extractive solution 3%～6%；
Step 2, ratio in described step one, by Buckwheat flour, light flour, sodium bicarbonate, glucono-δ-lactone, baking powder mix homogeneously；
Step 3, the xylitol under described step one ratio, Sal are joined in the water of lower 40 DEG C～50 DEG C of described step one ratio and dissolve, it is thus achieved that sugar aqueous solution；
Step 4, adding mixing in flour mixed in advance in described step 2 for the soybean oil of ratio in described step one, sucrose solution step 3 obtained adds in flour mixing and is modulated into dough.
Step 5, the dough standing 30min that will obtain in described step 4；
Step 6, the dough in described step 5 is carried out rolling；During rolling, repeatedly folding and half-twist, needs rolling 10 times～15 times, becomes the flour cake of consistency of thickness to dough rolling；
Step 7, the flour cake Biscuit mold described step 6 obtained make biscuit, are neatly placed in the baking tray coating oil, are placed in 200 DEG C of baking boxs, toast 6min～15min；
Step 8, by baking after cookies take out, naturally cool to room temperature；Cookies is loaded in vacuum bag and seal with vacuum pumping sealing port machine；
The making of eight-ingredient buckwheat porridge
In step one, BABAOZHOU, each ratio is: buckwheat 50%～70%, Semen phaseoli radiati 5%～20%, Semen Maydis quarrel 5%～10%, fructus zizaniae caduciflorae 5%～10%, Fructus Crataegi 1%～5%, Rhizoma Dioscoreae 1%～10%, Fructus Lycii 1%～10%, Poria 1%～10%；
Step 2, the material in described step one is removed clean after impurity standby；
Step 3, by described step 2 clean after material, be placed in water in the ratio in described step one, soak 2h～4h；
Step 4, the material after soaking in described step 3 is added water according to the ratio that material and water are 1:20; add sodium D-isoascorbate by 1g/kg, add sodium phosphate by 2g/kg and meet agent, steaming and decocting 10min～30min; after truce, stewing 10min, continues steaming and decocting 10min～30min；
Step 5, by material well-done in step 4, weighed weight after stirring, add after xylitol according to 40g/kg；Sterilize when 110 DEG C～120 DEG C, finished product packing.
Buckwheat flour in the described drying work of Radix Ginseng buckwheat cake includes Buckwheat flour and Testa Fagopyri Esculenti, and its portion rate is 7:3.
Sodium phosphate described in the making of described eight-ingredient buckwheat porridge meets the geometric ratio mixed liquor that the composition of agent is sodium pyrophosphate and sodium tripolyphosphate.
By above-mentioned design, the present invention can bring following beneficial effect: the extraction and analytical method of pyrite and application thereof in a kind of Semen Fagopyri Esculenti, after the extracting flavonoids in shelled buckwheat, Testa Fagopyri Esculenti, add the compositions such as ginsenoside, xylitol, Fructus Lycii essence, citric acid, blood vessel can be changed, improve microcirculation, improve contraction and the diastolic function of body vascular smooth muscle.
Accompanying drawing explanation
Below in conjunction with the drawings and specific embodiments, the present invention is further illustrated:
Fig. 1 is the extraction and analytical method schematic flow sheet of flavone in a kind of Semen Fagopyri Esculenti of the present invention.
Fig. 2 is the Making programme schematic diagram of the application buckwheat beverage of flavone in a kind of Semen Fagopyri Esculenti of the present invention.
Fig. 3 is the Making programme schematic diagram of the application Radix Ginseng Semen Fagopyri Esculenti cookies of flavone in a kind of Semen Fagopyri Esculenti of the present invention.
Fig. 4 is the Making programme schematic diagram of the application eight-ingredient buckwheat porridge of flavone in a kind of Semen Fagopyri Esculenti of the present invention.
Detailed description of the invention
The extraction and analytical method of pyrite and application thereof in a kind of Semen Fagopyri Esculenti, wherein in Semen Fagopyri Esculenti, the extraction and analytical method of flavone is as shown in Figure 1.
Embodiment 1: the mensuration of the extraction process of flavone and content in Semen Fagopyri Esculenti, shelled buckwheat, Testa Fagopyri Esculenti
Step one, flavone extraction: take and smash to pieces with a collection of Semen Fagopyri Esculenti, separate shelled buckwheat, Testa Fagopyri Esculenti.Take Semen Fagopyri Esculenti, shelled buckwheat, each 20g of Testa Fagopyri Esculenti respectively, pulverize, cross 60 mesh sieves.Semen Fagopyri Esculenti, shelled buckwheat, Testa Fagopyri Esculenti are put in conical flask respectively, adds 6 times amount water, decoct 2 times, each 2 hours.Merge decocting liquid, add 95% ethanol so that it is concentration, to 70%, stands 24 hours.Filter, collect filtrate, be settled to 2000ml and get final product.
Prepared by step 2, standard curve: precision weighs the rutin standard substance 6.0mg of 120 DEG C of dry constant weights, adds 70% ethanol and dissolves, is settled in 50ml volumetric flask, shakes up, make the standard solution that concentration is 0.12mg/ml.Taking standard solution 1.0ml, 2.0ml, 3.0ml, 4.0ml, 5.0ml respectively in 25ml volumetric flask, respectively add water to 5ml, precision adds 5% sodium nitrite 1.0ml, shakes up, and places 6min；Add 10% aluminum nitrate 1.0ml, shake up, place 6min；The sodium hydroxide l0ml adding 4%, shakes up, and places l5min, parallel reagent blank.In 510nm wavelength place, measure absorbance.
Step 3: sample size measures.Accurate absorption Semen Fagopyri Esculenti need testing solution 3ml, shelled buckwheat need testing solution 3ml, Testa Fagopyri Esculenti need testing solution 1ml, in 25ml volumetric flask, respectively add water to 5ml, and precision adds 5% sodium nitrite 1.0ml, shakes up, and places 6min；Add 10% aluminum nitrate 1.0ml, shake up, place 6min；Add 4% sodium hydroxide l0ml, shake up, place l5min, parallel reagent blank.In 510nm wavelength place, measure absorbance.
Result is as shown in the table:
| Numbering | Concentration (mg/ml) | Reading value |
| Standard specimen 1 | 0.004 | 0.0473 |
| Standard specimen 2 | 0.008 | 0.0978 |
| Standard specimen 3 | 0.012 | 0.1394 |
| Standard specimen 4 | 0.016 | 0.1836 |
| Standard specimen 5 | 0.020 | 0.2218 |
Wherein, regression equation: y=10.87x+0.0075；Correlation coefficient: r=0.9989
According to data above, calculate each several part flavones content as follows: Semen Fagopyri Esculenti 10.00mg/g；Shelled buckwheat 6.67mg/g；Testa Fagopyri Esculenti 20.00mg/g.
By data above it can be seen that Semen Fagopyri Esculenti each several part all contains abundant flavones ingredient, wherein the flavones content of Testa Fagopyri Esculenti is the highest, is about 3 times of shelled buckwheat, for 2 times of Semen Fagopyri Esculenti.
Embodiment 2: the making of buckwheat beverage, as in figure 2 it is shown,
The extraction of buckwheat flavonoids:
Step one, take sweet cherry roots skin, with the aqueous sodium carbonate (PH=9) of 15 times amount, supersound extraction 3 times, each 30min, centrifugal, take supernatant；
Step 2, the centrifugal liquid of step one gained being concentrated into the 1/2 of original volume, the hydrochloric acid with 10% adjusts PH=4, by the concentrated solution after having adjusted, lets cool, static 48h, is centrifuged, taking precipitate；
Step 3, take the precipitate of step 2 gained, be put in drying under reduced pressure case, 40 DEG C of drying under reduced pressure, obtain buckwheat flavonoids.
The preparation of buckwheat beverage:
Step one, take buckwheat flavonoids 60mg, with potassium carbonate (0.1mol/L) solution, ultrasonic 10min, all dissolve to buckwheat flavonoids.
Step 2, take the buckwheat flavonoids solution of gained in step one, Radix Ginseng 6g, separately take xylitol 15g, potassium carbonate 10mg, citric acid 0.2g, Fructus Lycii essence 0.5ml, cyclamate 0.4g, add pure water 1000ml, mix homogeneously；
Step 3, by the mixed solution of step 2 gained, carry out membrane filtration, sterilizing in 150 μm of semipermeable membranes, obtain buckwheat beverage.
Embodiment 3: the making of Radix Ginseng Semen Fagopyri Esculenti cookies, as it is shown on figure 3,
In step one, cookies, each formula proportion is as follows: Buckwheat flour 84g (includes Buckwheat flour and Testa Fagopyri Esculenti, ratio is 7:3), light flour 42g, soybean oil 15g, baking powder 2.56g, sodium bicarbonate 1.25g, glucono-δ-lactone 1.6g, Sal 0.5g, xylitol 20g, water 33.75g, Radix Ginseng extractive solution 8g.
Step 2, in step one ratio, by Buckwheat flour, light flour, sodium bicarbonate, glucono-δ-lactone, baking powder mix homogeneously.
Step 3, the xylitol under step one ratio, Sal are joined in the hot water under step one ratio and dissolves, obtain sugar aqueous solution.
Step 4, the soybean oil of ratio in step one is added mix homogeneously in flour mixed in advance in step 2, then sucrose solution step 3 obtained adds in flour mix homogeneously and modulates agglomerating.
Step 5, the dough standing 30min that will obtain in step 4.
Step 6, the dough in step 6 is carried out rolling.During rolling, repeatedly folding and half-twist, needs rolling more than 10 time, until dough becomes, thickness is uniform, form is smooth, the flour cake of smooth surface and exquisite quality.
Step 7, flour cake step 6 obtained, make difform animal pattern biscuit with Biscuit mold, is neatly placed in the baking tray coating oil.
Step 8, in 200 DEG C of baking boxs, baking 15min.
Step 9, baked cookies is slowly cooled to room temperature.Finally cookies is loaded in vacuum bag and seal with vacuum pumping sealing port machine.
Embodiment 4: the making of eight-ingredient buckwheat porridge, as shown in Figure 4,
In step one, BABAOZHOU, each ratio is as follows: buckwheat 60g, Semen phaseoli radiati 9g, Semen Maydis quarrel 5g, fructus zizaniae caduciflorae 5g, Fructus Crataegi 3g, Rhizoma Dioscoreae 6g, Fructus Lycii 6g, Poria 6g.
Step 2, material in step one is removed after impurity, clean up, standby.
Step 3, the material that will clean up in step 2, be placed in water in ratio in step one, soaks 3h, makes material softening.
Step 4, step 3 will be soaked the material of deliquescing add drinking water according to the ratio of 1:20.Add sodium D-isoascorbate by 1g/kg, add sodium phosphate by 2g/kg and meet agent (sodium pyrophosphate and the mixing of 4 sodium tripolyphosphate geometric ratios), steaming and decocting 20 minutes, cease fire stewing 10 minutes again, continue steaming and decocting 20 minutes.
Step 5, by material well-done in step 4, stir, weighed weight, according to 40g/kg add xylitol, mixing.
Step 6, high temperature sterilization, finished product packing, to obtain final product.
Claims (4)
1. an extraction and analytical method for flavone in Semen Fagopyri Esculenti, is characterized in that: comprise the following steps,
Step one, flavone extraction
Take Semen Fagopyri Esculenti to smash to pieces, separate shelled buckwheat, Testa Fagopyri Esculenti；Take Semen Fagopyri Esculenti 20g, shelled buckwheat 20g, Testa Fagopyri Esculenti 20g respectively, after pulverizing, cross 60 mesh sieves；Semen Fagopyri Esculenti after sieving, shelled buckwheat, Testa Fagopyri Esculenti are respectively placed in conical flask, in conical flask, add 6 times amount water, decoct 2 times, each 2 hours, merge decocting liquid, add 95% ethanol, concentration is regulated to 70%, filter after standing 24 hours, collect filtrate, be settled to 2000ml and obtain need testing solution；
Step 2, prepare standard curve
Weigh the rutin standard substance 6.0mg of 120 DEG C of dry constant weights, add the alcoholic solution that concentration is 70% and dissolve, be settled in 50ml volumetric flask and mix, make the standard solution that concentration is 0.12mg/ml；Take standard solution 1.0ml, 2.0ml, 3.0ml, 4.0ml, 5.0ml respectively and be placed in 25ml volumetric flask, add water to 5ml respectively, after dosing the sodium nitrite 1.0ml mixing that concentration is 5%, stand 6min；6min is stood after being separately added into the aluminum nitrate solution 1.0ml mixing of 10%；L5min, parallel reagent blank is stood after being separately added into the sodium hydroxide solution 10ml mixing of 4%；It is respectively placed in 510nm wavelength place, measures absorbance；
Step 3, sample size measure
Take Semen Fagopyri Esculenti need testing solution 3ml, shelled buckwheat need testing solution 3ml, Testa Fagopyri Esculenti need testing solution 1ml that described step one obtains, be placed in 25ml volumetric flask, add water to 5ml respectively, after adding 5% sodium nitrite 1.0ml mixing, place 6min；6min is stood after adding the aluminum nitrate 1.0ml mixing that concentration is 10%；L5min, parallel reagent blank is stood after adding the sodium hydroxide solution l0ml mixing that concentration is 4%；It is respectively placed in 510nm wavelength place, measures absorbance.
2. an application for flavone in Semen Fagopyri Esculenti, is characterized in that: include the making of the making of buckwheat beverage, the making of Radix Ginseng Semen Fagopyri Esculenti cookies and eight-ingredient buckwheat porridge；
The making of buckwheat beverage
Step one, taking sweet cherry roots skin, add the aqueous sodium carbonate of the PH=9 of 10～20 times amount, carry out supersound extraction 3 times, each extraction time is 10min～50min, takes supernatant after centrifugal；
Step 2, the centrifugal liquid that described step one obtains being concentrated into the 1/2 of original volume, the hydrochloric acid with 10% regulates to PH=4, by the concentrated solution after regulating, static placement 12h～48h, taking precipitate after centrifugal；
Step 3, take the precipitate that described step 2 obtains, be put in drying under reduced pressure case, when 40 DEG C～60 DEG C of 10Pa～30Pa, dry 8h～12h, it is thus achieved that buckwheat flavonoids；
Step 4, take described step 3 obtain buckwheat flavonoids 50mg～80mg, add 0.1mol/L solution of potassium carbonate, after carrying out ultrasonic dissolution 10min～30min, it is thus achieved that buckwheat flavonoids is completely dissolved liquid；
Step 5, take in described step 4 obtain buckwheat flavonoids be completely dissolved liquid, Radix Ginseng 3g～6g, separately taking xylitol 5g～15g, potassium carbonate 5mg～10mg, citric acid 0.05g～0.2g, Fructus Lycii essence 0.5ml～1.5ml, cyclamate 0.1g～0.4g, after adding pure water 1000ml, mixing obtains mixed liquor；
Step 6, the mixed solution described step 5 obtained, carry out membrane filtration, sterilizing through 150 μm of semipermeable membranes, obtain buckwheat beverage；
The making of Radix Ginseng Semen Fagopyri Esculenti cookies
In step one, cookies, each formula proportion is: Buckwheat flour 15%～40%, light flour 30%～60%, soybean oil 8%～12%, baking powder 2%～4%, sodium bicarbonate 1%～2%, glucono-δ-lactone 1%～3%, Sal 0.1%～0.4%, xylitol 10%～16%, water 25%～30%, Radix Ginseng extractive solution 3%～6%；
Step 2, ratio in described step one, by Buckwheat flour, light flour, sodium bicarbonate, glucono-δ-lactone, baking powder mix homogeneously；
Step 3, the xylitol under described step one ratio, Sal are joined in the water of lower 40 DEG C～50 DEG C of described step one ratio and dissolve, it is thus achieved that sugar aqueous solution；
Step 4, adding mixing in flour mixed in advance in described step 2 for the soybean oil of ratio in described step one, sucrose solution step 3 obtained adds in flour mixing and is modulated into dough.
Step 5, the dough standing 30min that will obtain in described step 4；
Step 6, the dough in described step 5 is carried out rolling；During rolling, repeatedly folding and half-twist, needs rolling 10 times～15 times, becomes the flour cake of consistency of thickness to dough rolling；
Step 7, the flour cake Biscuit mold described step 6 obtained make biscuit, are neatly placed in the baking tray coating oil, are placed in 200 DEG C of baking boxs, toast 6min～15min；
Step 8, by baking after cookies take out, naturally cool to room temperature；Cookies is loaded in vacuum bag and seal with vacuum pumping sealing port machine；
The making of eight-ingredient buckwheat porridge
In step one, BABAOZHOU, each ratio is: buckwheat 50%～70%, Semen phaseoli radiati 5%～20%, Semen Maydis quarrel 5%～10%, fructus zizaniae caduciflorae 5%～10%, Fructus Crataegi 1%～5%, Rhizoma Dioscoreae 1%～10%, Fructus Lycii 1%～10%, Poria 1%～10%；
Step 2, the material in described step one is removed clean after impurity standby；
Step 3, by described step 2 clean after material, be placed in water in the ratio in described step one, soak 2h～4h；
Step 4, the material after soaking in described step 3 is added water according to the ratio that material and water are 1:20; add sodium D-isoascorbate by 1g/kg, add sodium phosphate by 2g/kg and meet agent, steaming and decocting 10min～30min; after truce, stewing 10min, continues steaming and decocting 10min～30min；
Step 5, by material well-done in step 4, weighed weight after stirring, add after xylitol according to 40g/kg；Sterilize when 110 DEG C～120 DEG C, finished product packing.
3. the application of flavone in a kind of Semen Fagopyri Esculenti according to claim 1, is characterized in that: the Buckwheat flour in the described drying work of Radix Ginseng buckwheat cake includes Buckwheat flour and Testa Fagopyri Esculenti, and its portion rate is 7:3.
4. the application of flavone in a kind of Semen Fagopyri Esculenti according to claim 1, is characterized in that: sodium phosphate described in the making of described eight-ingredient buckwheat porridge meets the geometric ratio mixed liquor that the composition of agent is sodium pyrophosphate and sodium tripolyphosphate.
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| CN108813615A (en) * | 2018-07-05 | 2018-11-16 | 中国农业科学院农产品加工研究所 | The preparation method of mung bean extracting solution with antioxidant activity |
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Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1537562A (en) * | 2003-04-16 | 2004-10-20 | 郑向黎 | Compound flavone capsule and pellet prepn. method |
| CN101019677A (en) * | 2007-03-20 | 2007-08-22 | 陕西师范大学 | Sugar-less buckwheat flavone beverage |
| CN101045720A (en) * | 2007-03-20 | 2007-10-03 | 陕西师范大学 | Method for extracting buckwheat flavone from buckwheat shell |
| CN101455371A (en) * | 2008-11-27 | 2009-06-17 | 成都润馨堂药业有限公司 | Health-care product containing buckwheat flavone and preparation method thereof |
| CN101791085A (en) * | 2010-04-23 | 2010-08-04 | 刘学发 | Functional eight-ingredient buckwheat porridge and method for making same |
| CN102641343A (en) * | 2012-05-05 | 2012-08-22 | 耿福能 | Water-soluble tartary buckwheat general flavone extract and preparation method and application thereof |
| CN103250769A (en) * | 2013-05-09 | 2013-08-21 | 王永琪 | Health care food therapy cookies for chronic rhinitis |
| CN103284056A (en) * | 2013-06-28 | 2013-09-11 | 安徽燕之坊食品有限公司 | Nutritional coarse cereal porridge and preparation method thereof |
| CN103478567A (en) * | 2013-10-08 | 2014-01-01 | 南昌大学 | Glucose-control porridge and preparation technology |
| CN103875782A (en) * | 2014-04-21 | 2014-06-25 | 马国丰 | Method for making mixed flour nutritious biscuits |
| CN104069187A (en) * | 2014-07-24 | 2014-10-01 | 劲牌生物医药有限公司 | Technological method extracting duckwheat flavone from duckwheat |
| CN104543730A (en) * | 2015-02-11 | 2015-04-29 | 同福碗粥股份有限公司 | Porridge suitable for patients with diabetes and preparation method of porridge |
-
2016
- 2016-04-08 CN CN201610217023.7A patent/CN105795480A/en active Pending
Patent Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1537562A (en) * | 2003-04-16 | 2004-10-20 | 郑向黎 | Compound flavone capsule and pellet prepn. method |
| CN101019677A (en) * | 2007-03-20 | 2007-08-22 | 陕西师范大学 | Sugar-less buckwheat flavone beverage |
| CN101045720A (en) * | 2007-03-20 | 2007-10-03 | 陕西师范大学 | Method for extracting buckwheat flavone from buckwheat shell |
| CN101455371A (en) * | 2008-11-27 | 2009-06-17 | 成都润馨堂药业有限公司 | Health-care product containing buckwheat flavone and preparation method thereof |
| CN101791085A (en) * | 2010-04-23 | 2010-08-04 | 刘学发 | Functional eight-ingredient buckwheat porridge and method for making same |
| CN102641343A (en) * | 2012-05-05 | 2012-08-22 | 耿福能 | Water-soluble tartary buckwheat general flavone extract and preparation method and application thereof |
| CN103250769A (en) * | 2013-05-09 | 2013-08-21 | 王永琪 | Health care food therapy cookies for chronic rhinitis |
| CN103284056A (en) * | 2013-06-28 | 2013-09-11 | 安徽燕之坊食品有限公司 | Nutritional coarse cereal porridge and preparation method thereof |
| CN103478567A (en) * | 2013-10-08 | 2014-01-01 | 南昌大学 | Glucose-control porridge and preparation technology |
| CN103875782A (en) * | 2014-04-21 | 2014-06-25 | 马国丰 | Method for making mixed flour nutritious biscuits |
| CN104069187A (en) * | 2014-07-24 | 2014-10-01 | 劲牌生物医药有限公司 | Technological method extracting duckwheat flavone from duckwheat |
| CN104543730A (en) * | 2015-02-11 | 2015-04-29 | 同福碗粥股份有限公司 | Porridge suitable for patients with diabetes and preparation method of porridge |
Non-Patent Citations (6)
| Title |
|---|
| 新加坡AP出版社有限公司: "《韩国（第二版）》", 30 September 2007, 中国水利水电出版社 * |
| 李静舒: "苦荞总黄酮提取工艺的比较研究", 《陕西广播电视大学学报》 * |
| 柴瑞霞: "《容易受伤的女人 常见妇科病的防治与食疗》", 30 June 2014, 江西科学技术出版社 * |
| 甘智荣: "《糖尿病不发愁》", 30 June 2015, 吉林科学技术出版社 * |
| 邹亮等: "苦荞黄酮提取与分离技术的研究进展", 《安徽农业科学》 * |
| 韩志萍等: "甜荞麦不同部位总黄酮含量测定", 《食品研究与开发》 * |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108813615A (en) * | 2018-07-05 | 2018-11-16 | 中国农业科学院农产品加工研究所 | The preparation method of mung bean extracting solution with antioxidant activity |
| CN108813615B (en) * | 2018-07-05 | 2022-06-07 | 中国农业科学院农产品加工研究所 | Preparation method of mung bean extract with antioxidant activity |
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| CN113678973A (en) * | 2021-08-06 | 2021-11-23 | 微肽科技(海南)有限公司 | Noni enzyme collagen peptide composite beverage and preparation method thereof |
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