CN105793278A - 肽基甘氨肽α酰胺化单氧加氧酶(PAM)用于C端酰胺化的用途 - Google Patents

肽基甘氨肽α酰胺化单氧加氧酶(PAM)用于C端酰胺化的用途 Download PDF

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Publication number
CN105793278A
CN105793278A CN201480066822.8A CN201480066822A CN105793278A CN 105793278 A CN105793278 A CN 105793278A CN 201480066822 A CN201480066822 A CN 201480066822A CN 105793278 A CN105793278 A CN 105793278A
Authority
CN
China
Prior art keywords
polypeptide
pam
nucleic acid
amidatioon
pyy
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201480066822.8A
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English (en)
Chinese (zh)
Inventor
E·霍夫曼
G·蒂芬塔勒
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Publication of CN105793278A publication Critical patent/CN105793278A/zh
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/003—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by transforming the C-terminal amino acid to amides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/17—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with reduced ascorbate as one donor, and incorporation of one atom of oxygen (1.14.17)
    • C12Y114/17003—Peptidylglycine monooxygenase (1.14.17.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y403/00—Carbon-nitrogen lyases (4.3)
    • C12Y403/02—Amidine-lyases (4.3.2)
    • C12Y403/02005—Peptidylamidoglycolate lyase (4.3.2.5)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Analytical Chemistry (AREA)
  • Endocrinology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
CN201480066822.8A 2013-12-20 2014-12-10 肽基甘氨肽α酰胺化单氧加氧酶(PAM)用于C端酰胺化的用途 Pending CN105793278A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP13199222.4 2013-12-20
EP13199222 2013-12-20
PCT/EP2014/077143 WO2015091131A1 (en) 2013-12-20 2014-12-10 Use of peptidylglycine alpha-amidating monooxigenase (pam) for c-terminal amidation

Publications (1)

Publication Number Publication Date
CN105793278A true CN105793278A (zh) 2016-07-20

Family

ID=49885043

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201480066822.8A Pending CN105793278A (zh) 2013-12-20 2014-12-10 肽基甘氨肽α酰胺化单氧加氧酶(PAM)用于C端酰胺化的用途

Country Status (10)

Country Link
US (1) US20160333386A1 (ja)
EP (1) EP3083662A1 (ja)
JP (1) JP2017507645A (ja)
KR (1) KR20160098278A (ja)
CN (1) CN105793278A (ja)
BR (1) BR112016012325A2 (ja)
CA (1) CA2928927A1 (ja)
MX (1) MX2016007202A (ja)
RU (1) RU2016129516A (ja)
WO (1) WO2015091131A1 (ja)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4110372A1 (en) * 2020-02-26 2023-01-04 PAM Theragnostics GmbH Use of peptidylglycine alpha-amidating monooxygenase (pam) for therapeutic purpose
EP4111201A1 (en) * 2020-02-26 2023-01-04 PAM Theragnostics GmbH Methods for determining peptidylglycine alpha-amidating monooxygenase (pam) and its use for diagnostic purpose

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PA8660701A1 (es) * 2005-02-04 2006-09-22 Pfizer Prod Inc Agonistas de pyy y sus usos
US8008453B2 (en) * 2005-08-12 2011-08-30 Amgen Inc. Modified Fc molecules
ES2583259T3 (es) * 2009-12-01 2016-09-20 Novo Nordisk A/S Nuevas liasas alfa-amidantes de peptidil alfa-hidroxiglicina

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
KAYOKO TATEISHI等: "Isolation and functional expression of human pancreatic peptidylglycine α-amidating monooxygenase", 《BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS》 *
KEN-ICHIRO TAKAHASHI等: "Production of Bioactive Salthe Nonendocrine Cell Lines COS-7 and CHOmon Calcitonin From", 《PEPTIDES》 *
ZHEN LIU等: "Cloning, Co-Expression with an Amidating Enzyme, and Activity of the Scorpion Toxin BmK ITa1 cDNA in Insect Cells", 《MOLECULAR BIOTECHNOLOGY》 *

Also Published As

Publication number Publication date
RU2016129516A (ru) 2018-01-25
BR112016012325A2 (pt) 2017-09-26
MX2016007202A (es) 2016-07-21
US20160333386A1 (en) 2016-11-17
JP2017507645A (ja) 2017-03-23
KR20160098278A (ko) 2016-08-18
CA2928927A1 (en) 2015-06-25
EP3083662A1 (en) 2016-10-26
WO2015091131A1 (en) 2015-06-25

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