CN105777322A - Liquid potato seed dressing agent and preparation method thereof - Google Patents

Liquid potato seed dressing agent and preparation method thereof Download PDF

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Publication number
CN105777322A
CN105777322A CN201510879298.2A CN201510879298A CN105777322A CN 105777322 A CN105777322 A CN 105777322A CN 201510879298 A CN201510879298 A CN 201510879298A CN 105777322 A CN105777322 A CN 105777322A
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parts
potato
seed
dressing agent
seed dressing
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CN201510879298.2A
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赵洪军
赵庆涛
马强
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Abstract

The invention provides a preparation method for a potato seed dressing agent. The preparation method has the following advantages: 1, the method prepares a bioactive extract through microwave-assisted secondary crushing, synergistic extraction of three algae active components by using an enzyme hydrolysis method, and double-strain liquid co-fermentation, wherein the bioactive extract is rich in bioactive components like natural organic matters, nitrogen-phosphorus-potassium, active strains, algae polysaccharide and mannitol, so nutrients and effective bioactive bacteria are directly provided for the cutting wound surface of the potato, and cutting wound healing is greatly promoted; 2, the seed dressing agent has a film forming characteristic and acts on the surface layer of seed potato to form a natural bioactive protective film which provides protection for the seed potato in the stages of sowing to germination and is naturally-degradable and residue-free; and 3, an application method for the seed dressing agent comprises liquid spraying or seed dressing, and compared with a traditional seed dressing way, the method has the advantages of safety, environmental protection, simplicity, high efficiency, convenient operation, and conservation of effort and labor.

Description

A kind of liquid potato seed-dressing agent and preparation method thereof
Technical field
The preparation method that the present invention relates to a kind of potato seed-dressing agent, belongs to biologic product technology field.
Background technology
Rhizoma Solani tuber osi (formal name used at school: Solanumtuberosum), belongs to Solanaceae herbaceos perennial, and tuber can be edible, is the fourth-largest important cereal crops in the whole world, is only second to Semen Tritici aestivi, Oryza glutinosa and Semen Maydis, simultaneously the fourth-largest cereal crops of Ye Shi China.
2010, the world wide production of Rhizoma Solani tuber osi reached 3.24 hundred million tons, and China is the first in the world yield big country, and potato yield reaches 75,000,000 tons then.The main producing region of potatoes is Southwest Mountainous Areas, northwest, the Inner Mongol and the Northeast.Compared with Semen Tritici aestivi, Semen Maydis, Oryza sativa L., potato full-powder storage time is longer, can store at normal temperatures more than 15 years, and some countries are classified as Rhizoma Solani tuber osi as strategic reserves grain.2015, China will start Rhizoma Solani tuber osi staple food grainization strategy, advance and Potato ring rot bacteria be become the staple foods such as steamed bread, noodles, rice flour, and the another staple food grain that Rhizoma Solani tuber osi will become outside rice, Semen Tritici aestivi, Semen Maydis has important strategic importance.
In potato culture process, often occur that plant diminishes year by year, leaf-shrinkage is curling, leaf colour shading is uneven, short and small thin and delicate, the tuber deformation be full of cracks of stem stalk, yield descend degradation phenomenon year by year, showing that Rhizoma Solani tuber osi has occurred and that degeneration, it is cause the main cause that yield reduces and commodity property is deteriorated that potato seed is degenerated.Research potato seed degeneration Crack cause, finds and solves potato degeneration technology, be once an important topic of world research Rhizoma Solani tuber osi.Research worker from plant physiology, biochemistry, cultivation technique, kind block processes, pest and disease damage such as infects at the aspect, potato degeneration is conducted in-depth research, finally specify that virus infect and in potato block, accumulation is the main cause of potato degeneration, Common Diseases has late blight in seedling stage, tar spot, dry rot.Potato block as lower generation " seed " suffers virus infection and accumulation because of reasons such as handling, transport, storage, potato seed strippings and slicings, and not there is the mechanism of self purged body inner virus, cause that plant virosis increases the weight of year by year, make plant can not give full play to the industry characteristics of kind in process of production, cause Severe Reduction.Only adopt modern biotechnology the virus in potato seed to be removed, recover physiological function and the industry characteristics of Potato Cultivars itself, just can prevent the degeneration of Rhizoma Solani tuber osi, make up to commodity property and the yield of kind.
For the infringement preventing virus infection potato seed potato block from potato growth being caused, common method is to utilize seed dressing that potato block is dressed seed, conventional seed dressing has the violent zinc of plant ash, streptomycin, metalaxyl, Mancozeb, carbendazim, Sandofan etc., dressed seed by various compatible combination, complicated operation, easily bring secondary virus infection, and the use of its agricultural chemicals is likely in plant to be enriched with, human body is adversely affected.Development of new safety, environmental protection, novel agricultural potato seed-dressing agent easy and simple to handle have become as the task of top priority.
Summary of the invention
For making up existing potato seed-dressing agent deficiency in actual production operates, the invention provides a kind of liquid potato seed-dressing agent, it is therefore intended that alleviate potato seed potato block virus infection, it is ensured that potato plant normal growth, improve plant resistance, improve yield;Walk quickly and keep away subterranean pest-insect simultaneously, prevent soil-borne disease, resist peasant household and plant the continuous cropping disease that Rhizoma Solani tuber osi is caused throughout the year.
For achieving the above object, the technical solution used in the present invention is as follows:
The invention provides a kind of liquid potato seed-dressing agent, it is characterised in that: raw material and the proportion of composing of the lixiviating solution of one of seed dressing component are as follows: 10~25 parts of Thallus Laminariae (Thallus Eckloniae), ulva 8~15 parts, Alga Sgrgassi Enerves 15~20 parts, corn straw 20~35 parts, dry rice straw 3~8 parts, plant ash 5~10 parts, glucose 10~15 parts, the peat composed of rotten mosses 5~10 parts, molasses fermented liquid 8~12 parts, soybean cake 10~12 parts, yeast powder 5~8 parts, cellulase 3~5 parts, pectase 2~3 parts.
The preparation method that the invention provides a kind of liquid potato seed-dressing agent, it is characterised in that: above-mentioned raw materials prepares the lixiviating solution preparation for potato seed-dressing agent by " microwave-assisted second-time breakage+enzymatic isolation method three Sargassum active component collaborative lixiviate+two microorganisms liquid ferment altogether ".
Above-mentioned lixiviating solution production technology, realizes particular by following approach:
(1) Thallus Laminariae (Thallus Eckloniae), ulva, Alga Sgrgassi Enerves, corn straw are all removed and are rotted and the person of going mouldy, and dry and shearing-crushing, cross 50 mesh sieves;
(2) according to aforementioned proportion compatibility raw material, 2 times of tap waters are added, broken 30~40min under microwave action, Extracting temperature controls at 60~90 DEG C, and broken microwave proceeds enzymolysis after terminating, the controlled enzymatic hydrolysis time is at 6~8h, mixed liquor filter pressing, filtrate is transferred to fermentation tank;
(3) mixeding liquid temperature is down to 25 DEG C~30 DEG C inoculation bacillus subtilises 2~3 parts and bacillus megaterium 1~2 part, keeps temperature 30 DEG C~40 DEG C reaction 18~22h, filters and obtain lixiviating solution.
The invention provides a kind of liquid potato seed-dressing agent, it is characterised in that: the liquid film former preparation method of one of seed dressing component is as follows:
(1) by 10~15 parts of sodium carboxymethyl cellulose, 3~5 parts of copper sulphate pentahydrate, 8~10 parts of ferrous sulfate, 8~10 parts of potassium sulfate mix homogeneously, it is slowly added in 100 parts of tap waters under stirring, utilization is steam heated to 70~90 degree, all dissolve to sodium carboxymethyl cellulose, obtain carboxymethylcellulose sodium solution.
(2) take 10~20 parts of carboxymethylcellulose sodium solutions, 3~5 parts of glycerol mixing, obtain liquid film former.
The invention provides a kind of liquid potato seed-dressing agent, it is characterised in that: potato seed-dressing agent is to be achieved in the following manner:
Taking 55~70 portions of lixiviating solution, 25~30 parts of liquid film former, 1~2 part of OP-10,1~2 part of sodium benzoate mix and blend 15min~30min, get product liquid potato seed-dressing agent.
The preparation method that the invention provides a kind of potato seed-dressing agent, this product is liquid, and color is pale yellow transparent shape, and good fluidity has the special odor of Sargassum.Uniformly sprayed by nebulization in potato seed potato block surface, or seed soaking, the potato seed potato block after process is placed in the dry in the sun of dry earth's surface and within 2~3 days, can sow.
The preparation method that the invention provides a kind of potato seed-dressing agent, it is advantageous that: one is that the present invention passes through " microwave-assisted second-time breakage+enzymatic isolation method three Sargassum active component collaborative lixiviate+two microorganisms liquid altogether ferment " and prepares biological activity lixiviating solution, rich in bioactive ingredients such as natural organic matter, N P and K, active bacteria, Sargassum polysaccharides, mannitol in this lixiviating solution, nutrition and effective bio-active bacteria (fungi) are directly provided for potato cutting wound face, have been greatly facilitated stripping and slicing wound healing;Two is that this seed dressing has film forming characteristics, acts on seed potato top layer, forms natural bioactive protecting film, provides protection for potato block in sowing to germination stage, and can natural degradation, noresidue;Three be this seed dressing using method is that liquid sprays or dresses seed, relatively conventional seed dressing mode, safety and environmental protection, simply efficiently, it is simple to operation, saves labor.
Detailed description of the invention
Below in conjunction with embodiment, the present invention will be further described.
Embodiment 1:
A kind of liquid potato seed-dressing agent, is prepared by following steps:
(1) prepared by lixiviating solution: take 10 parts of Thallus Laminariae (Thallus Eckloniae), ulva 8 parts, Alga Sgrgassi Enerves 15 parts, corn straw 20 parts, dry rice straw 3 parts, plant ash 5 parts, glucose 10 parts, the peat composed of rotten mosses 5 parts, molasses fermented liquid 8 parts, soybean cake 10 parts, yeast powder part, cellulase 3 parts, pectase 2 parts, wherein Thallus Laminariae (Thallus Eckloniae), ulva, Alga Sgrgassi Enerves, corn straw are all removed and are rotted and the person of going mouldy, dry and shearing-crushing, cross 50 mesh sieves;Adding 2 times of tap waters in reaction system, broken 30~40min under microwave action, Extracting temperature controls at 60~90 DEG C, and broken microwave proceeds enzymolysis after terminating, and the controlled enzymatic hydrolysis time, mixed liquor filter pressing, filtrate was transferred to fermentation tank at 6~8h;Mixeding liquid temperature is down to 25 DEG C~30 DEG C inoculation bacillus subtilises 2 parts and bacillus megaterium 2 parts, keeps temperature 30 DEG C~40 DEG C reaction 18~22h, filters and obtain lixiviating solution.
(2) prepared by liquid film former: by 10 parts of sodium carboxymethyl cellulose, 3 parts of copper sulphate pentahydrate, 8 parts of ferrous sulfate, 8 parts of potassium sulfate mix homogeneously, it is slowly added in 100 parts of tap waters under stirring, utilization is steam heated to 70~90 degree, all dissolve to sodium carboxymethyl cellulose, obtain carboxymethylcellulose sodium solution;Take 10 parts of carboxymethylcellulose sodium solutions, 3 parts of glycerol mixing, obtain liquid film former.
(3) prepared by seed dressing: taking 55 portions of lixiviating solution, 25 parts of liquid film former, 1 part of OP-10,1 part of sodium benzoate mix and blend 15min~30min, get product liquid potato seed-dressing agent.
Embodiment 2:
A kind of liquid potato seed-dressing agent, is prepared by following steps:
(1) prepared by lixiviating solution: take 15 parts of Thallus Laminariae (Thallus Eckloniae), ulva 10 parts, Alga Sgrgassi Enerves 18 parts, corn straw 20 parts, dry rice straw 5 parts, plant ash 6 parts, glucose 10 parts, the peat composed of rotten mosses 8 parts, molasses fermented liquid 8 parts, soybean cake 10 parts, yeast powder 5 parts, cellulase 5 parts, pectase 2 parts, wherein Thallus Laminariae (Thallus Eckloniae), ulva, Alga Sgrgassi Enerves, corn straw are all removed and are rotted and the person of going mouldy, dry and shearing-crushing, cross 50 mesh sieves;Adding 2 times of tap waters in reaction system, broken 30~40min under microwave action, Extracting temperature controls at 60~90 DEG C, and broken microwave proceeds enzymolysis after terminating, and the controlled enzymatic hydrolysis time, mixed liquor filter pressing, filtrate was transferred to fermentation tank at 6~8h;Mixeding liquid temperature is down to 25 DEG C~30 DEG C inoculation bacillus subtilises 2 parts and bacillus megaterium 2 parts, keeps temperature 30 DEG C~40 DEG C reaction 18~22h, filters and obtain lixiviating solution.
(2) prepared by liquid film former: by 12 parts of sodium carboxymethyl cellulose, 3 parts of copper sulphate pentahydrate, 8 parts of ferrous sulfate, 10 parts of potassium sulfate mix homogeneously, it is slowly added in 100 parts of tap waters under stirring, utilization is steam heated to 70~90 degree, all dissolve to sodium carboxymethyl cellulose, obtain carboxymethylcellulose sodium solution;Take 15 parts of carboxymethylcellulose sodium solutions, 3 parts of glycerol mixing, obtain liquid film former.
(3) prepared by seed dressing: taking 60 portions of lixiviating solution, 25 parts of liquid film former, 1 part of OP-10,2 parts of sodium benzoate mix and blend 15min~30min, get product liquid potato seed-dressing agent.
Embodiment 3:
A kind of liquid potato seed-dressing agent, is prepared by following steps:
(1) prepared by lixiviating solution: take 20 parts of Thallus Laminariae (Thallus Eckloniae), ulva 10 parts, Alga Sgrgassi Enerves 18 parts, corn straw 30 parts, dry rice straw 5 parts, plant ash 8 parts, glucose 12 parts, the peat composed of rotten mosses 8 parts, molasses fermented liquid 10 parts, soybean cake 12 parts, yeast powder 5 parts, cellulase 4 parts, pectase 2 parts, wherein Thallus Laminariae (Thallus Eckloniae), ulva, Alga Sgrgassi Enerves, corn straw are all removed and are rotted and the person of going mouldy, dry and shearing-crushing, cross 50 mesh sieves;Adding 2 times of tap waters in reaction system, broken 30~40min under microwave action, Extracting temperature controls at 60~90 DEG C, and broken microwave proceeds enzymolysis after terminating, and the controlled enzymatic hydrolysis time, mixed liquor filter pressing, filtrate was transferred to fermentation tank at 6~8h;Mixeding liquid temperature is down to 25 DEG C~30 DEG C inoculation bacillus subtilises 3 parts and bacillus megaterium 2 parts, keeps temperature 30 DEG C~40 DEG C reaction 18~22h, filters and obtain lixiviating solution.
(2) prepared by liquid film former: by 12 parts of sodium carboxymethyl cellulose, 3 parts of copper sulphate pentahydrate, 10 parts of ferrous sulfate, 8 parts of potassium sulfate mix homogeneously, it is slowly added in 100 parts of tap waters under stirring, utilization is steam heated to 70~90 degree, all dissolve to sodium carboxymethyl cellulose, obtain carboxymethylcellulose sodium solution;Take 15 parts of carboxymethylcellulose sodium solutions, 3 parts of glycerol mixing, obtain liquid film former.
(3) prepared by seed dressing: taking 70 portions of lixiviating solution, 30 parts of liquid film former, 1 part of OP-10,1 part of sodium benzoate mix and blend 15min~30min, get product liquid potato seed-dressing agent.
Above example is merely to illustrate technical scheme, but not is limited;Although being described in detail by invention with reference to previous embodiment, but for the person of ordinary skill of the art, still the technical scheme described in previous embodiment can be modified, or wherein portion of techniques feature is carried out equivalent replacement;And to these amendments or replacement, do not make the essence of appropriate technical solution depart from the spirit and scope of present invention technical scheme required for protection.

Claims (3)

1. a potato seed-dressing agent, is prepared by following steps:
(1) prepared by lixiviating solution: take 10~25 parts of Thallus Laminariae (Thallus Eckloniae), ulva 8~15 parts, Alga Sgrgassi Enerves 15~20 parts, corn straw 20~35 parts, dry rice straw 3~8 parts, plant ash 5~10 parts, glucose 10~15 parts, the peat composed of rotten mosses 5~10 parts, molasses fermented liquid 8~12 parts, soybean cake 10~12 parts, yeast powder 5~8 parts, cellulase 3~5 parts, pectase 2~3 parts, wherein Thallus Laminariae (Thallus Eckloniae), ulva, Alga Sgrgassi Enerves, corn straw are all removed and are rotted and the person of going mouldy, dry and shearing-crushing, cross 50 mesh sieves;Adding 2 times of tap waters in reaction system, broken 30~40min under microwave action, Extracting temperature controls at 60~90 DEG C, and broken microwave proceeds enzymolysis after terminating, and the controlled enzymatic hydrolysis time, mixed liquor filter pressing, filtrate was transferred to fermentation tank at 6~8h;Mixeding liquid temperature is down to 25 DEG C~30 DEG C inoculation bacillus subtilises 2~3 parts and bacillus megaterium 1~2 part, keeps temperature 30 DEG C~40 DEG C reaction 18~22h, filters and obtain lixiviating solution.
2.(2) prepared by liquid film former: by 10~15 parts of sodium carboxymethyl cellulose, 3~5 parts of copper sulphate pentahydrate, 8~10 parts of ferrous sulfate, 8~10 parts of potassium sulfate mix homogeneously, it is slowly added in 100 parts of tap waters under stirring, utilization is steam heated to 70~90 degree, all dissolve to sodium carboxymethyl cellulose, obtain carboxymethylcellulose sodium solution;Take 10~20 parts of carboxymethylcellulose sodium solutions, 3~5 parts of glycerol mixing, obtain liquid film former.
3.(3) taking 55~70 portions of lixiviating solution, 25~30 parts of liquid film former, 1~2 part of OP-10,1~2 part of sodium benzoate mix and blend 15min~30min, get product liquid potato seed-dressing agent.
CN201510879298.2A 2015-12-05 2015-12-05 Liquid potato seed dressing agent and preparation method thereof Pending CN105777322A (en)

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103553828A (en) * 2013-11-12 2014-02-05 天津北洋百川生物技术有限公司 Liquid compound biological fertilizer with long shelf life as well as preparation method and application thereof
CN104292939A (en) * 2014-07-14 2015-01-21 Pac生命科技研发有限公司 Alga extraction liquid capable of dispersing large-cluster molecules and preparation method thereof
CN104447008A (en) * 2014-12-05 2015-03-25 中国科学院烟台海岸带研究所 Method for preparing liquid seaweed fertilizer
CN105010402A (en) * 2015-07-06 2015-11-04 领先生物农业股份有限公司 Suspended biological seed coating agent and preparation method and application thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103553828A (en) * 2013-11-12 2014-02-05 天津北洋百川生物技术有限公司 Liquid compound biological fertilizer with long shelf life as well as preparation method and application thereof
CN104292939A (en) * 2014-07-14 2015-01-21 Pac生命科技研发有限公司 Alga extraction liquid capable of dispersing large-cluster molecules and preparation method thereof
CN104447008A (en) * 2014-12-05 2015-03-25 中国科学院烟台海岸带研究所 Method for preparing liquid seaweed fertilizer
CN105010402A (en) * 2015-07-06 2015-11-04 领先生物农业股份有限公司 Suspended biological seed coating agent and preparation method and application thereof

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