CN105770377B - Chinese herbal medicine compound for resisting drying damage and application thereof in cosmetics - Google Patents
Chinese herbal medicine compound for resisting drying damage and application thereof in cosmetics Download PDFInfo
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- CN105770377B CN105770377B CN201410830037.7A CN201410830037A CN105770377B CN 105770377 B CN105770377 B CN 105770377B CN 201410830037 A CN201410830037 A CN 201410830037A CN 105770377 B CN105770377 B CN 105770377B
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Abstract
The invention discloses a Chinese herbal medicine with an anti-drying damage effect and application thereof in cosmetics. Specifically, the invention discloses an application of a fresh lily peanut rehmannia root extract in dry damage resistance, and an application of the fresh lily peanut rehmannia root extract in cosmetics as an additive with a dry damage resistance effect.
Description
Technical Field
The invention aims to provide a Chinese herbal medicine compound extract with an anti-drying injury effect, which can resist the injury of skin cells caused by drying and maintain the healthy and active state of the skin cells. Meanwhile, the composition can be used as an effect additive to be added into cosmetics for skin care, and the influence of dryness factors on the skin is reduced.
Background
The skin is located at the outermost layer of the human body and is exposed to the air, so that the skin is difficult to be protected from various environments, and adverse factors can accelerate skin aging and cause skin damage, such as cold wind, dry indoor environment and the like, at the moment, the moisture content in the air is reduced, the air is dry, and the moisture in the skin can evaporate to the external environment. When the moisture in the skin is insufficient, the skin is excessively dehydrated, skin cells are damaged, the vitality of the skin is reduced, and the skin is dried. Therefore, the protection under the dry environment is not negligible for the skin, and the research and development of the additive with excellent effect for resisting dry damage is of great significance. The evaluation method adopted by the research mainly simulates the dehydration state of the skin in a dry environment in daily life, and damages the epidermal cells. The anti-drying injury medicine screened by the method can better keep the moisture of the skin and is beneficial to the moisturizing effect of the skin.
Lily flower is sweet, slightly bitter and slightly cold in nature. It enters lung, liver and heart meridians. According to ancient books, the effects of clearing heat, moistening lung, calming heart and soothing nerves are recorded; sheng Di Huang is sweet and bitter in taste and cold in nature, and enters heart, liver and kidney meridians, so it is an essential herb for clearing heat and cooling blood, and also has the actions of nourishing yin, clearing blood heat, and treating fever and diabetes. The two herbs are combined together to achieve the action of cooling and moistening, and they are incorporated into the lung meridian. The traditional Chinese medicine considers that the lung governs the skin and hair, and the anthers have the property of promoting hair growth, so the traditional Chinese medicine is suitable for treating diseases outside the upper part, is frequently applied to facial skin diseases, and the rehmannia root can be used for promoting the growth of lily, and is more suitable for facial external application. The dryness is caused by the invasion of dryness pathogen, the traditional Chinese medicine theory considers that the dryness is easy to generate fire, and the lily and the peanut are both cold and cool medicines and have the effects of entering lung channels, clearing heat and cooling blood and cooling head and face.
Chinese patent application CN201110007654.3 describes a Chinese medicinal composition for treating sjogren's syndrome. The invention belongs to the field of traditional Chinese medicines, is used for treating sicca syndrome, and mainly comprises the following traditional Chinese medicine components in parts by weight: 20g of each of radix adenophorae and radix ophiopogonis, 10-15 g of each of rhizoma ligustici wallichii, wild chrysanthemum, radix paeoniae alba, schisandra chinensis, radix asparagi, radix polygonati officinalis, dendrobe, radix rehmanniae, fructus amomi and pollen and 6g of liquorice. Has the effects of nourishing yin, benefiting qi, promoting blood circulation and dredging collaterals, and has exact curative effect on sicca syndrome. The application has the defects that: the Sicca Syndrome (SS) referred to herein is as described herein: is a chronic inflammatory autoimmune disease mainly affecting the exocrine glands. It is characterized by dry mouth, eye and other mucous membranes, and often rheumatic diseases (such as RA, scleroderma and SLE) with certain autoimmune features, with the evidence of lymphocyte infiltration in the affected tissues, and involvement of organs of the whole body caused by involvement of multiple systems of the kidney, lung, nervous system, digestive system, etc. It is manifested by muscle weakness, general soreness, dry cough, chest distress, epilepsy, flaccid paralysis, hepatitis with unknown reasons, liver cirrhosis, chronic diarrhea, etc. First, the sicca syndrome mentioned in this patent application is a completely different concept than the dry skin lesions mentioned in the present invention. Secondly, the patent relates to the combined use of a plurality of traditional Chinese medicines, and although rehmannia root and lily are mentioned, the traditional Chinese medicines are not core medicines and are not used singly in pairs. Moreover, the medicinal materials used in the application are not the fresh medicines of the invention.
Taiwan patent application TWI445542 describes medical effect and its use in the presence of white soot liquid as the active ingredient. The present invention provides a novel pro-filaggrin or a filaggrin production promoter, a encrusting protein production promoter, a cholesterol production promoter and a cell activator derived from natural products. It is a pro-filaggrin or a filaggrin production promoter, a encrusted protein production promoter, a cholesterol production promoter and a cell activation agent, which contain a birch sap as an active ingredient. However, the patent application mainly protects the white birch sap, only mentions radix rehmanniae recen and lily among optional moisturizers, but does not mention a certain proportion of combination and dry injury resistance, and the used medicinal materials are not limited to the fresh medicine of the invention.
Chinese patent application CN200480005008.1 describes antioxidant compositions and topical compositions. The application provides a technical method for realizing new functions by applying fullerene and derivatives thereof to various fields suitable for body applications, in particular to a method for applying the fullerene and the derivatives thereof to cosmetics and external skin prescription drugs, and an antioxidant composition is prepared by using at least 1 of fullerene, fullerene oxygen-containing derivatives, and fullerene or fullerene oxygen-containing derivatives modified or contained by organic compounds as active ingredients. However, the patent application only mentions that the humectant can be selected from lily and radix rehmanniae extract, but does not mention a certain proportion of combination, does not mention drying damage resistance, and the used medicinal materials are not limited to the fresh medicine of the invention.
Chinese patent application cn200480006830.x describes a skin external preparation characterized by containing a saccharide derivative of α, α -trehalose. The application provides substances having blood circulation promoting, anti-inflammatory, antibacterial, moisturizing, whitening, ultraviolet absorbing, ultraviolet scattering, and antioxidant effects; substances with hair nourishing-hair growth effects; an external preparation for skin which is excellent in emulsification, astringency, anti-wrinkle, cell activation and/or percutaneous absorption-promoting action, safe and comfortable to use. The above object is achieved by providing any 1 or 2 or more kinds of external preparations for skin selected from the group consisting of a saccharide derivative of α, α -trehalose and a substance having a blood circulation-promoting action, an anti-inflammatory action substance, an antibacterial action substance, a moisturizing action substance, a whitening action substance, an ultraviolet absorbing action substance, an external ray scattering action substance, an antioxidant action substance, a substance having a hair-nourishing and hair-growing action, a substance having an emulsifying action, a substance having an astringent action, a substance having an anti-wrinkle action, a substance having a cell-activating action and/or a percutaneous absorption-promoting action. However, the patent application only mentions that the moisturizing agent can be selected from lily and radix rehmanniae extract, but does not mention a certain proportion of combination and dry injury resistance, and the used medicinal materials are not limited to the fresh medicines of the invention.
Chinese patent application CN200580020086.3 discloses an inflammation inhibitor containing zinc salts of acylamino acids. The purpose of this application is to provide an inflammation inhibitor that gives a good feeling of use. Disclosed is a skin inflammation inhibitor characterized by containing a zinc salt of an acylamino acid, and a cosmetic material characterized by containing the inflammation inhibitor. However, the patent application only mentions that the humectant can be selected from lily and radix rehmanniae extract, but does not mention a certain proportion of combination, does not mention drying damage resistance, and the used medicinal materials are not limited to the fresh medicine of the invention.
Chinese patent application CN201210056434.4 discloses a Chinese medicine with the functions of maintaining beauty and keeping young. The application relates to a traditional Chinese medicine with the effects of maintaining beauty and keeping young, which is prepared from the following medicinal materials in parts by weight: 200-400 parts of wolfberry fruit, 10-20 parts of schisandra chinensis, 400-600 parts of fried Chinese yam, 100-300 parts of honey, 6-12 parts of dark plum, 10-20 parts of medicinal cyathula root, 10-20 parts of fried white paeony root, 10-20 parts of dogwood, 10-20 parts of pawpaw, 8-15 parts of radix rehmanniae, 60-70 parts of donkey-hide gelatin, 50-70 parts of phaseolus calcaratus, 10-20 parts of lily, 9-30 parts of coix seed, 9-15 parts of poria cocos, 6-12 parts of fried bighead atractylodes rhizome, 10-20 parts of cortex lycii radicis, 8-12 parts of platycodon grandi. Clinical tests prove that the invention can maintain beauty and keep young, and has safe taking and no toxic or side effect. However, although the application of the patent mentions that the extracts of lily and radix rehmanniae can be selected as the traditional Chinese medicine composition for maintaining beauty and keeping young, the application does not mention the combination of the extracts in pairs and a certain proportion, and the application for maintaining beauty and keeping young does not disclose the specific efficacy and does not relate to the dry injury resistance. Meanwhile, the used medicinal materials are not limited to the fresh medicines of the invention.
In addition, natural plants for cosmetics (Shaojian chapter, Chinese wild plant resources, 1998,01:22-24) disclose that with the improvement of living and cultural levels of people, beauty, skin and hair care are more and more valued, and that the selection of pure natural plant components as cosmetic additives for nutrition, hair care, face nourishing, skin moistening and aging delaying are not something of a few people. The cosmetic must have the effects of moistening, protecting and nourishing skin and hair, enhancing the vitality of epidermal cells, promoting metabolism, stretching wrinkles, building the skin and beautifying the skin, and also has the effect of removing dirt. Plants which have a cosmetic, skin-and hair-caring effect are described. Similarly, while rehmannia is indicated to have a nourishing effect on the skin, it is not mentioned to combat drying damage, nor is it disclosed to be used in combination with lily in a certain ratio. Meanwhile, the used medicinal materials are not limited to the fresh medicines of the invention.
The application of medicinal plants in cosmetics (Gejie, Shandong Chinese medicine journal, 2005,12: 747-. The development, utilization and research of the medicinal plant cosmetics have wide development prospect. The following is a description of the use of several commonly used medicinal plants in cosmetics. Similarly, although the document discloses that lily has the effects of increasing skin nutrition and promoting skin metabolism, the document does not mention the dry injury resistance, and does not disclose the combination of lily and dried rehmannia root in a certain proportion. Meanwhile, the used medicinal materials are not limited to the fresh medicines of the invention.
From these contents, although there are reports about lily and radix rehmanniae as moisturizers in the prior art, there is no report about the use of fresh lily, peanut and radix rehmanniae in pairs based on the theory and fresh application of traditional Chinese medicine. In addition, the problem of skin dryness is mostly considered from the viewpoint of external moisturizers, and no solution has been proposed in the aspect of emphasizing resistance to dryness damage and maintenance of cell health and vitality from the aspect of skin cells.
Disclosure of Invention
The invention firstly proposes that the fresh lily, peanut and rehmannia root medicines are used in pairs, and the obtained fresh medicine extract can resist the injury of skin cells caused by dryness and maintain the healthy and active state of the skin cells. Meanwhile, the fresh medicinal extract of the lily, the peanut and the rehmannia can also be used as an efficacy additive to be added into cosmetics for skin care, so that the influence of drying factors on the skin is reduced.
The extraction process flow of the lily, the peanut and the rehmannia is as follows:
soaking flos Lilii Viriduli in suitable solvent for about 0.5-1h, extracting by corresponding method, collecting filtrate, standing, filtering, concentrating to about 2 times (V/W) of the total amount of the medicinal materials, and making into solution containing 0.1-0.5g/ml of the medicinal materials.
Soaking radix rehmanniae in suitable solvent for about 0.5-1h, extracting by corresponding method, collecting filtrate, standing, filtering, concentrating to about 2 times (V/W) of total amount of the medicinal materials, and making into solution containing crude drug 0.1-0.5 g/ml.
Mixing the lily extract and the radix rehmanniae extract in proportion to obtain a compound solution with crude drug content of 0.1-0.5 g/ml.
The traditional Chinese medicinal materials in the formula can be properly enlarged or reduced according to the proportion; suitable solvents include water (distilled, deionized, purified), ethanol solutions of varying concentrations, polyol solutions of varying concentrations, and other solvents of similar polarity; the extraction method can be decocting, reflux extracting, soaking, percolating, etc.
Therefore, in one aspect of the present invention, a method for preparing a fresh herb extract of lily, peanut and rehmannia is provided, which comprises the following steps:
a) soaking fresh flos Lilii Viriduli in solvent, extracting, filtering, concentrating, drying into powder to obtain fresh flos Lilii Viriduli extract,
b) soaking fresh radix rehmanniae in solvent, extracting, filtering, concentrating, drying to obtain fresh radix rehmanniae extract,
c) combining the fresh lily extract and the fresh rehmannia root extract obtained in the steps a) and b) to obtain the fresh lily peanut and rehmannia root extract.
In one embodiment, the vehicle is selected from: water, ethanol solution, and polyalcohol solution.
In one embodiment, the extraction is selected from: decocting, reflux extracting, soaking, and percolating.
In one embodiment, the fresh lily herb extract and the fresh rehmannia root extract in step c) are combined in a ratio of 7:3 to 3: 7.
In another aspect, the invention also relates to a fresh medicinal extract of lily, peanut and rehmannia for resisting drying damage. The fresh medicine extract is prepared according to the method of the invention. In one embodiment, the weight ratio of lily to radix rehmanniae in the fresh lily, peanut and radix rehmanniae extract is 7:3 to 3: 7.
On the other hand, the invention also relates to the application of the fresh lily, peanut and rehmannia root extract in the aspect of protecting skin against dry injury.
On the other hand, the invention also relates to the application of the fresh lily, peanut and rehmannia root extract as an additive with the effect of resisting drying damage in cosmetics. In one embodiment, the cosmetic is selected from: face cleaning lotion, cosmetic water, lotion, cream, jelly and facial mask.
In one embodiment, the fresh extract of lily, peanut, rehmannia glutinosa is used in an amount of 0.00001 to 100 wt% of the cosmetic. In another embodiment, the fresh extract of Lilium, Arachis hypogaea, Digitalis purpurea is used in an amount of 0.0001-50 wt% of the cosmetic. In a preferred embodiment, the amount of the fresh extract of lily, peanut, rehmannia glutinosa used in the cosmetic is 0.001-20 wt%.
Drawings
Fig. 1A shows a microscope image (× 10) of blank well cells observed before drying.
Fig. 1B shows a microscope image (× 10) of blank well cells observed after drying.
Figure 1C shows a microscopic image (× 10) of 1% compound extract a well cells observed before drying.
Figure 1D shows a microscope image (× 10) of 1% compound extract a well cells observed after drying.
Figure 1E shows a microscopic image (× 10) of 1% co-extract B-well cells observed before drying.
Figure 1F shows a microscope image (× 10) of 1% co-extract B-well cells observed after drying.
Figure 1G shows a microscope image (× 10) of 1% co-extract C well cells observed before drying.
Figure 1H shows the microscopic image (x 10) observed after drying of 1% compound extract C well cells.
Figure 1I shows a microscope image (× 10) of 1% lily extract well cells observed before drying.
Fig. 1J shows a microscope image (× 10) of 1% lily extract well cells observed after drying.
FIG. 1K shows a microscopic image (. times.10) of 1% rehmannia glutinosa extract well cells observed before drying.
FIG. 1L shows a microscope image (. times.10) of 1% rehmannia glutinosa extract well cells observed after drying.
FIG. 2 shows the results of dry cell death (%) obtained in the in vitro epidermal keratinocyte dry damage resistance test. Wherein # indicates a significant difference in blank group relative to the normal group without drying treatment; indicates significant difference (p <0.05) relative to the blank group; indicates a very significant difference (p <0.01) from the blank group.
Detailed Description
The inventors of the present invention have found, after extensive and intensive studies: the fresh medicinal extract of lily, peanut and rehmannia has the function of resisting dryness and injury and can maintain the healthy and active state of skin cells. Therefore, the fresh medicinal extract of the lily, the peanut and the rehmannia can be used as an efficacy additive to be added into cosmetics for skin care and reducing the influence of dryness factors on the skin.
The literature does not relate to the effect of the lily, peanut and rehmannia compound fresh medicine extract on resisting drying injury, and does not mention that the lily, peanut and rehmannia compound fresh medicine extract is added into cosmetics as a functional additive. Therefore, the invention firstly provides that the fresh medicinal extract of lily, peanut and rehmannia has the effect of resisting dry injury, and meanwhile, the fresh medicinal extract can also be used as a functional additive to be added into cosmetics for skin care.
In cosmetic applications, the fresh extract of lily, peanut, rehmannia glutinosa may be in various forms. For example, the application form of the fresh medicinal extract of lily, peanut and rehmannia can be any dosage forms which can be applied to human skin, such as facial cleanser, cosmetic water, lotion, cream, jelly, facial mask and the like.
The other ingredients besides the fresh drug extract of lily, peanut, rehmannia can be any ingredients required by the cosmetic formula, such as: water, ethanol, butanediol, propylene glycol, oil, emulsifier, thickener, antiseptic, etc.; other functional components can also be added to achieve compound functions, such as: adding sunscreen components to obtain sunscreen cosmetic for promoting energy metabolism, adding oil control components to obtain oil control cosmetic for promoting energy metabolism, adding moisturizing components to obtain moisturizing cosmetic for promoting energy metabolism, adding whitening components to obtain whitening cosmetic for promoting energy metabolism, and adding antiaging components to obtain antiaging cosmetic for promoting energy metabolism.
Generally, the fresh extract of lily, peanut, rehmannia root can be used at a concentration of 0.00001 to 100% by weight. Preferably, the fresh extract of lily, peanut and rehmannia root can be used at a concentration of 0.0001-50 wt%. In one embodiment of the invention, the fresh extract of lily, peanut, rehmannia glutinosa is used at a concentration of 0.00001 to 100% by weight. In another embodiment, the concentration of the fresh lily, peanut and rehmannia root extract is from 0.001 to 50% by weight. In one embodiment, the fresh herb extract of lily, peanut, rehmannia glutinosa is used at a concentration of 0.001-20 wt%.
The fresh medicinal extract of Bulbus Lilii, semen Arachidis Hypogaeae and rehmanniae radix can be added into various cosmetics at a ratio of 0.00001-100 wt%. For example, the fresh extract of lily, peanut, rehmannia root may be used in an amount of 0.00001 to 25% by weight, such as 0.0001 to 50% by weight, such as 0.001 to 75% by weight, such as 0.1 to 100% by weight. In a preferred embodiment, the fresh extract of lily, peanut and rehmannia glutinosa is used in an amount of 0.0001-50 wt% in the cosmetic. In another preferred embodiment, the fresh extract of lily, peanut and rehmannia glutinosa is used in an amount of 0.001-20 wt% in the cosmetic.
The weight ratio of the lily extract to the radix rehmanniae extract in the fresh lily, peanut and radix rehmanniae extract can be any ratio. For example, in one embodiment, the weight ratio of the lily extract to the rehmannia glutinosa extract in the fresh lily peanut rehmannia glutinosa extract is 3: 7.
The invention is further illustrated below with reference to specific examples. It is to be understood, however, that these examples are illustrative only and are not to be construed as limiting the scope of the present invention. Test methods in which specific conditions are not specified in the following examples are generally carried out under conventional conditions or under conditions recommended by the manufacturer. All percentages and parts are by weight unless otherwise indicated.
Example 1: preparation of fresh medicine extract of lily, peanut and rehmannia
Weighing a proper amount of fresh lily, extracting with 11 times of deionized water → filtering, passing the filtrate through a membrane separation device to obtain a solution, concentrating the solution to 2 times, and drying into powder to obtain the fresh lily extract.
Weighing a proper amount of fresh rehmannia root, extracting with 8 times of deionized water → filtering, concentrating the filtrate, adding ethanol for precipitating → filtering, concentrating the filtrate to 0.125 times, and drying into powder to obtain the rehmannia root extract.
Mixing 3 parts of fresh lily extract and 7 parts of fresh rehmannia root extract according to a proportion to finally prepare the compound extract A with the weight of 1 percent.
Mixing 7 parts of fresh lily extract and 3 parts of fresh rehmannia root extract according to a proportion to finally prepare a compound extract B containing 1 percent of weight.
Mixing 1 part of fresh lily extract and 1 part of fresh rehmannia root extract in proportion to finally prepare the compound extract C with the weight of 1 percent.
Preparing 1% (5mg/ml) of the fresh drug extract of lily, peanut and rehmannia to obtain a sample solution.
Example 2: in vitro anti-dessication injury test of epidermal cells
Epidermal cell in vitro anti-drying injury test was performed using fresh lily herb extract, fresh rehmannia root herb extract, fresh lily peanut rehmannia root extract A, B and C prepared in example 1. The specific experimental procedures are as follows:
(1) after synchronization of the cells, 2.5 x 104One/well concentration was seeded in 24-well plates.
(2) Except for the blank (wells of cells not dosed before drying), the corresponding sample was added to each well and incubated for 24 hours.
(3) At room temperature, each well of the medium was sequentially aspirated off and photographed (x 10 fold) under a microscope imaging system at the corresponding time point (after 0min, same drying time). The microscope model used was OLYMPUS IX 50.
The test results are shown in FIG. 1. The results show that:
(1) after being placed indoors for an equivalent period of time, the non-medicated blank cell group cells are obviously shrunk, deformed and increased in refractive index. The cells of the experimental group added with the fresh medicinal extracts A and B of lily, peanut and rehmannia basically maintain the normal healthy state of full water, no obvious change is caused before drying, only a small part of the compound extract C changes, and the effect is only inferior to that of the compound extracts A and B.
(2) After being placed indoors for a period of time, the experimental group cells singly added with lily and radix rehmanniae all have certain damage, shrinkage, deformation and refractive index increase. These results indicate that neither the extract of fresh lily nor the extract of fresh rehmannia root alone can protect epidermal cells from dry damage. On the contrary, when the extracts of lily and fresh rehmannia root are combined into a compound extract for use, the effect of resisting drying injury is shown in figure 1. Therefore, the lily and fresh rehmannia root extract compound has a synergistic effect, and the compound extract of the lily and fresh rehmannia root extract compound has an effect of resisting drying injury.
Example 3: experiment on in vitro epidermal keratinocyte for resisting dry injury
1. Preparation of experimental samples:
weighing a proper amount of fresh lily, extracting with 11 times of deionized water → filtering, passing the filtrate through a membrane separation device to obtain a solution, concentrating the solution to 2 times, and drying into powder to obtain the fresh lily extract.
Weighing a proper amount of fresh rehmannia root, extracting with 8 times of deionized water → filtering, concentrating the filtrate, adding ethanol for precipitating → filtering, concentrating the filtrate to 0.125 times, and drying into powder to obtain the rehmannia root extract.
Mixing 3 parts of fresh lily extract and 7 parts of fresh rehmannia root extract according to a proportion to finally prepare the compound extract containing 1 percent of weight.
Preparing the fresh medicinal extract of lily, peanut and rehmannia with the concentration of 0.1 percent (0.5mg/ml) to obtain a sample solution.
2. The experimental method comprises the following steps:
1) recovering the cells, and carrying out cell synchronization treatment when the growth state is good.
2) After synchronization, the concentration was adjusted and seeded in cell plates. After the cells adhere to the wall, adding prepared samples with different concentrations, and incubating for 24 hours after 3 multiple wells of each group.
3) The next day, in the super clean bench, drying at room temperature at a speed of 0.3-0.5 m/s, sequentially removing all culture medium except normal group, and standing in the super clean bench for 5-15 min. Then adding normal culture medium to continue culturing and incubating for 24 h.
4) MTT assay.
3. The experimental results are as follows:
and recording the OD value of each hole, averaging 3 multiple holes in each group, analyzing the experimental result by using statistical software STATA 8.0, and performing statistical treatment such as t test, variance analysis and the like. P <0.05, indicating significant difference; p <0.01 indicates that the difference is very significant. And the dry cell death rate (%) and the protective rate (%) were calculated according to the following formulas.
Cell dry mortality (%) [1- (blank/drug) OD value/normal blank OD value ]. 100%;
protection (%) - (blank cell dry death-drug group cell dry death)/blank cell dry death 100%.
The results of cell dry mortality are shown in FIG. 2. The results show a significant difference in the dry cell mortality rate of the blank group relative to the non-dried normal group. Meanwhile, compared with the blank group, the fresh lily, peanut and rehmannia root extract has a significant difference in cell dry death rate (p < 0.05). Therefore, the fresh lily, peanut and rehmannia root extract can protect cells from being damaged by dryness.
The results of the protection rate are shown in the following table.
| Additive agent | 0.1% of lily and dried rehmannia root | 0.1% of lily | 0.1% dried rehmannia root |
| Protective Rate (%) | 32.32％* | -18％ | -1.4％ |
The results show that:
the cells incubated with 0.1% of lily and 0.1% of radix rehmanniae alone have no difference through statistical analysis, and do not show good water retention and moisturizing effects in vitro. On the contrary, the 0.1% lily and the radix rehmanniae are adopted to incubate the cells, so that the effects of keeping water and moisture and resisting dry injury are better. Therefore, the fresh lily, peanut and rehmannia medicine extract prepared by the invention has the effect of drying damage resistance, and the medicine has a synergistic effect on the extract.
Example 4: preparation of anti-drying damage moisturizing cream
| Composition (I) | % concentration (by weight) |
| Extract of lily, peanut and rehmannia glutinosa | 1.0 |
| Glycerol stearate citrate | 4.0 |
| Glycerol stearate | 2.0 |
| Cetostearyl alcohol | 2.0 |
| Mineral oil | 10.0 |
| Caprylic/capric triglyceride | 5.0 |
| 1, 3-butanediol | 7.0 |
| Antiseptic and essence | Proper amount of |
| Water (W) | To 100.0 |
The preparation method comprises the following steps: dissolving the extract of lily, peanut and rehmannia glutinosa with 1, 3-butanediol, adding into water, and stirring to obtain water phase. Adding glycerol stearate citrate, glycerol stearate, cetearyl alcohol, caprylic/capric triglyceride and mineral oil into an oil phase pot, heating to 75-80 deg.C, and stirring to obtain oil phase. Slowly adding the oil phase into the water phase, stirring, adding antiseptic and essence, and stirring.
Example 5: preparation of anti-drying damage moisturizing lotion
| Composition (I) | % concentration (by weight) |
| Anti-drying damage moisturizing extract | 2.0 |
| 1, 3-butanediol | 8.0 |
| Antiseptic and water soluble essence | Proper amount of |
| Water (W) | To 100 |
The preparation method comprises the following steps: dissolving the extract of Bulbus Lilii, semen Arachidis Hypogaeae and rehmanniae radix with 1, 3-butanediol, adding into water, stirring, adding antiseptic and essence, and stirring.
Claims (5)
1. The application of the fresh lily peanut rehmannia root extract in the aspect of protecting skin from dry injury for non-treatment purposes, wherein the preparation method of the fresh lily peanut rehmannia root extract comprises the following steps:
a) soaking fresh flos Lilii Viriduli in solvent, extracting, filtering, concentrating, drying into powder to obtain fresh flos Lilii Viriduli extract,
b) soaking fresh radix rehmanniae in solvent, extracting, filtering, concentrating, drying to obtain fresh radix rehmanniae extract,
c) combining the fresh lily extract and the fresh rehmannia root extract prepared in the steps a) and b) to obtain a fresh lily peanut rehmannia root extract,
wherein the solvent is selected from: water, ethanol solution, polyol solution;
wherein, in the step c), the fresh lily herb extract and the fresh rehmannia root herb extract are combined according to the weight ratio of 7:3 to 3: 7.
2. The application of the fresh lily peanut rehmannia root extract as an additive with the functions of protecting skin and resisting dry damage in cosmetics, wherein the preparation method of the fresh lily peanut rehmannia root extract comprises the following steps:
a) soaking fresh flos Lilii Viriduli in solvent, extracting, filtering, concentrating, drying into powder to obtain fresh flos Lilii Viriduli extract,
b) soaking fresh radix rehmanniae in solvent, extracting, filtering, concentrating, drying to obtain fresh radix rehmanniae extract,
c) combining the fresh lily extract and the fresh rehmannia root extract prepared in the steps a) and b) to obtain a fresh lily peanut rehmannia root extract,
wherein the solvent is selected from: water, ethanol solution, polyol solution;
wherein, in the step c), the fresh lily herb extract and the fresh rehmannia root herb extract are combined according to the weight ratio of 7:3 to 3: 7.
3. The use of claim 1 or 2, wherein the extraction is selected from the group consisting of: decocting, reflux extracting, soaking, and percolating.
4. Use according to claim 2, characterized in that said cosmetic is selected from: face cleaning lotion, cosmetic water, lotion, cream, jelly and facial mask.
5. The use of claim 2, wherein said cosmetic comprises from 0.001% to 20% by weight of the fresh extract of lily, peanut, rehmannia root.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410830037.7A CN105770377B (en) | 2014-12-22 | 2014-12-22 | Chinese herbal medicine compound for resisting drying damage and application thereof in cosmetics |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410830037.7A CN105770377B (en) | 2014-12-22 | 2014-12-22 | Chinese herbal medicine compound for resisting drying damage and application thereof in cosmetics |
Publications (2)
| Publication Number | Publication Date |
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| CN105770377A CN105770377A (en) | 2016-07-20 |
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| JP2006347902A (en) * | 2005-06-14 | 2006-12-28 | Ogawa & Co Ltd | Moisture retention plant extract, and external preparation, cosmetic product, bathing agent and detergent containing the extract |
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