CN105766610B - A kind of seaweed spore aggregation method for culturing seedlings - Google Patents
A kind of seaweed spore aggregation method for culturing seedlings Download PDFInfo
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- CN105766610B CN105766610B CN201410822596.3A CN201410822596A CN105766610B CN 105766610 B CN105766610 B CN 105766610B CN 201410822596 A CN201410822596 A CN 201410822596A CN 105766610 B CN105766610 B CN 105766610B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The present invention relates to there is field of biotechnology, a kind of seaweed spore aggregation method for culturing seedlings is disclosed, by such as can while generate a large amount of spores at a high speed under cutting or High temperature to the seaweed environmental stimuli grown under natural conditions.It is cultivated under suitable conditions after the spore suspension that collection generates is concentrated.Under this condition of culture, spore grow in glass dish bottom with higher density and aggregation forms spore aggregation between each other.Ventilation is further cultured for develop at algae, another part spore aggregation can conservation through the low light intensity culture of low temperature after a part of spore aggregation is dispersed.Provided method can provide technical foundation for artificial large-scale cultivation seaweed.The main advantages of the present invention be to grow spore aggregation to suspend clustered pattern in culture solution, growth place is provided different from needing to provide seaweed spore or zygote etc. attachment in traditional nursery, in addition disperse agglomerations can provide guarantee for controllable seedling numbers in subsequent further expansion culture.
Description
Technical field
The present invention relates to field of biotechnology more particularly to a kind of seaweed spore aggregation method for culturing seedlings.
Background technique
China sea area is vast, and seaweed is resource important in ocean, as many kinds in red algae, green alga and brown alga have
Quite high economic value.But in recent years, with the exploitation to marine algae, the demand of algae increasingly increases, and sea area is natural
Wild edible seaweed has been far from satisfying the demand of market volume and quality.Therefore expand and carry out algal cultivation processing skill
Art is the important content of coastal state.
Artificial cultivation seaweed is mainly kelp, skirt dish, seaweed, fragrant plant mentioned in ancient texts, Eucheuma and reef film etc., major part both at home and abroad at present
Mainly take traditional algal cultivation mode.Traditional algal cultivation mode is to be greater than the former frond of 10cm using length in 20-
It is sprouted naturally at 30 DEG C and generates asexual spore or female and male gametophyte.Frond upper part after release starts gradually decline death occur
Putrefactive phenomenon.Since newly generated spore is smaller, at the same plus nature mariculture area there are water body, tide, season, water pollution,
Temperature, salinity altercation etc. influence the uncontrollable factor of spore survival rate, constrain new sporogenic growth and development, will cause cannot
Stablize and generates largely for harvesting at algae.
Although current main economic seaweed realizes artificial cultivation in various degree, the stability and matter of its yield
It still needs further improvement for amount.It is domestic at present that there has been no the correlations that seedling breeding is formed using seaweed spore aggregation suspension growth
Technology, therefore application of the development spore glomeration method in seaweed nursery is necessary in the method for obtaining the cultivation of seaweed seedling,
This method can provide technical foundation for artificial large-scale cultivation seaweed.
Summary of the invention
The present invention is directed to existing disadvantage, discloses a kind of seaweed spore aggregation method for culturing seedlings.
In order to solve the above-mentioned technical problem, the present invention is addressed by following technical proposals.
A kind of seaweed spore aggregation method for culturing seedlings, includes the following steps,
A. it takes frond to cut into segment, is cultivated after being cut into the sterilized seawer washing of frond of segment, generate spore；Or it will
After frond sterilizing seawer washing, in 20-25 DEG C, 100 μm of ol/m2It is cultivated under the illumination of/s, generates spore；Spore refers to asexual
Spore, female and male gametophyte.
B. the spore suspension that seaweed generates is collected, being concentrated into when being monitored with 10 × object lens of eyepiece 40 has in the visual field greater than 6
A spore, is cultivated again；It is stirred in incubation again, makes to be attached to each other aggregation growth between spore, form spore aggregation
Body；It is stirred in incubation again, makes to be attached to each other to assemble to grow with higher density between spore to form spore aggregation.
C. it collects spore aggregation and disperses, the spore aggregation seedling after the dispersion of formation goes to seedling as seedling
It ventilates and cultivates in flask, acquire the seedling of drag as deposit seedling.Spore a part after dispersion can be further cultured for through ventilation
Development is, at algae, another part is spare as deposit seedling；This condition of culture can make these spore balls at least survive 1 year or more simultaneously
And its growth is not observed.When seedling goes to ventilation culture in flask, suspension growth phenomenon can occur for part seedling.The present invention couple
Spore number control in spore aggregation after dispersion, and the present invention is suspended in culture solution and grows, and is different from tradition
Method is attached to wire side or rope growth, thus the phenomenon that yield reduces caused by avoiding because of harvesting difficulty.
Preferably, taking the frond grown under natural conditions in step A.
Preferably, in step A, it is cut into after the sterilized seawer washing of frond of segment to be put into illumination constant incubator and trains
It supports, the control of illumination constant incubator temperature is at 18-25 DEG C, and the photoperiod controls in 12L:12D, and intensity control is in 100 μm of ol/m2/
S is cultivated 2-5 days, generates spore.
Preferably, in step B when cultivating again, the spore suspension of concentration being put into illumination constant incubator and is trained
It supports, the control of illumination constant incubator temperature is at 18-25 DEG C, and the photoperiod controls in 12L:12D, and intensity control is in 100 μm of ol/m2/
S is cultivated 2-3 days.
Preferably, using magnetic stirrer in step B.
Preferably, in step C, when culture, seedling is put into illumination constant incubator and is cultivated, illumination constant incubator
Temperature control is at 18-25 DEG C, and the photoperiod controls in 12L:12D, and intensity control is in 100 μm of ol/m2/ s is cultivated 2-3 days.
Preferably, deposit seedling, which is put into illumination constant incubator, to be cultivated, illumination constant incubator temperature control in step C
System is at 10-15 DEG C, and the photoperiod controls in 12L:12D, and intensity control is in 10 μm of ol/m2/ s is cultivated 3 days, is stored for future use.After processing
Deposit seedling can be used as duration supply seedling source.Deposit seedling, which is put into illumination constant incubator, to be cultivated, the training of illumination constant temperature
The control of box temperature degree is supported at 10-15 DEG C, the photoperiod controls in 12L:12D, and intensity control is in 10 μm of ol/m2/ s, this condition of culture can
So that these seedling survivals 1 year or more, and its growth is not observed.
Compared with prior art, the invention has the benefit that
(1) seedling of " the spore aggregation " that the present invention uses as seaweed artificial cultivation initial stage.Its major advantage
Be to grow spore aggregation to suspend clustered pattern in culture solution, different from being needed in traditional nursery to seaweed spore or
Zygote etc. provides attachment and provides growth place, and in addition the step for disperse agglomerations can be for can in subsequent further expansion culture
Regulate and control seedling numbers and guarantee is provided.
(2) cultural method of the invention acquires spore, and easy to operate, the period is short, practical, and steady and sustained can receive
Collect a large amount of spores, is different from requiring harshness to frond maturity in traditional seedling raising manners, solve in traditional approach because of mature algae
Body discharges spore and the dead technical problem that fails.
(3) there are also so some for the superiority of spore clumps nursery: first, it is conducive to save, spore glomeration method is formed small
Seedling can save for a long time.Second, the seedling of formation is all clone, can convenient for whenever carry out production or test into
Row control.Third, the spore obtained by multiple phototaxis can form safe and reliable seedling to avoid the pollution of other algae
Kind.4th, high survival rate avoids extraneous factor such as natural enemy, and the factors such as environment transformation influence.
Specific embodiment
Embodiment 1
A kind of seaweed spore aggregation method for culturing seedlings, includes the following steps,
A. it takes the frond grown under natural conditions to cut into segment, after being cut into the sterilized seawer washing of frond of segment, puts
Enter and cultivated in illumination constant incubator, at 20 DEG C, the photoperiod controls in 12L:12D, light intensity the control of illumination constant incubator temperature
Control is in 100 μm of ol/m2/ s is cultivated 2 days, generates spore；
B. the spore suspension that seaweed generates is collected, being concentrated into when being monitored with 10 × object lens of eyepiece 40 has in the visual field greater than 6
A spore, is cultivated again；The spore suspension of concentration is put into illumination constant incubator and is cultivated, illumination constant incubator temperature
Control is at 20 DEG C, and the photoperiod controls in 12L:12D, and intensity control is in 100 μm of ol/m2/ s is cultivated 2 days；In incubation again
It is middle to use magnetic stirrer, make to be attached to each other aggregation growth between spore, forms spore aggregation；
C. it collects spore aggregation and disperses, the spore aggregation seedling after the dispersion of formation goes to seedling as seedling
It ventilates in flask culture, when culture, seedling is put into illumination constant incubator and is cultivated, the control of illumination constant incubator temperature exists
20 DEG C, the photoperiod controls in 12L:12D, and intensity control is in 100 μm of ol/m2/ s is cultivated 2 days；The seedling for acquiring drag makees
To lay in seedling；Deposit seedling, which is put into illumination constant incubator, to be cultivated, and for the control of illumination constant incubator temperature at 10-15 DEG C, light is all
Phase controls in 12L:12D, and intensity control is in 10 μm of ol/m2/ s is cultivated 2 days, is stored for future use.
Embodiment 2
A kind of seaweed spore aggregation method for culturing seedlings, includes the following steps,
A. it takes frond to cut into segment, after being cut into the sterilized seawer washing of frond of segment, is put into illumination constant incubator
Middle culture, the control of illumination constant incubator temperature is at 18 DEG C, and the photoperiod controls in 12L:12D, and intensity control is in 100 μm of ol/m2/
S is cultivated 5 days, generates spore；
B. the spore suspension that seaweed generates is collected, being concentrated into when being monitored with 10 × object lens of eyepiece 40 has in the visual field greater than 6
A spore, is cultivated again；The spore suspension of concentration is put into illumination constant incubator and is cultivated, illumination constant incubator temperature
Control is at 18 DEG C, and the photoperiod controls in 12L:12D, and intensity control is in 100 μm of ol/m2/ s is cultivated 3 days；In incubation again
It is middle to use magnetic stirrer, make to be attached to each other aggregation growth between spore, forms spore aggregation；
C. it collects spore aggregation and disperses, the spore aggregation seedling after the dispersion of formation goes to seedling as seedling
It ventilates in flask culture, when culture, seedling is put into illumination constant incubator and is cultivated, the control of illumination constant incubator temperature exists
18 DEG C, the photoperiod controls in 12L:12D, and intensity control is in 100 μm of ol/m2/ s is cultivated 3 days；
The seedling of drag is acquired as deposit seedling；Deposit seedling, which is put into illumination constant incubator, to be cultivated, illumination constant temperature
The control of incubator temperature is at 10 DEG C, and the photoperiod controls in 12L:12D, and intensity control is in 10 μm of ol/m2/ s is cultivated 2 days, is stored standby
With.
Embodiment 3
A kind of seaweed spore aggregation method for culturing seedlings, includes the following steps,
A. it takes the frond grown under natural conditions to cut into segment, after being cut into the sterilized seawer washing of frond of segment, puts
Enter and cultivated in illumination constant incubator, at 25 DEG C, the photoperiod controls in 12L:12D, light intensity the control of illumination constant incubator temperature
Control is in 100 μm of ol/m2/ s is cultivated 3 days, generates spore；
B. the spore suspension that seaweed generates is collected, being concentrated into when being monitored with 10 × object lens of eyepiece 40 has in the visual field greater than 9
A spore, is cultivated again；The spore suspension of concentration is put into illumination constant incubator and is cultivated, illumination constant incubator temperature
Control is at 25 DEG C, and the photoperiod controls in 12L:12D, and intensity control is in 100 μm of ol/m2/ s is cultivated 2.5 days；It was cultivating again
Magnetic stirrer is used in journey, is made to be attached to each other aggregation growth between spore, is formed spore aggregation；
C. it collects spore aggregation and disperses, the spore aggregation seedling after the dispersion of formation goes to seedling as seedling
It ventilates in flask culture, when culture, seedling is put into illumination constant incubator and is cultivated, the control of illumination constant incubator temperature exists
25 DEG C, the photoperiod controls in 12L:12D, and intensity control is in 100 μm of ol/m2/ s is cultivated 2.5 days；
The seedling of drag is acquired as deposit seedling；Deposit seedling, which is put into illumination constant incubator, to be cultivated, illumination constant temperature
The control of incubator temperature is at 10-15 DEG C, and the photoperiod controls in 12L:12D, and intensity control is in 10 μm of ol/m2/ s is cultivated 2 days, storage
It deposits spare.
Embodiment 4
A kind of seaweed spore aggregation method for culturing seedlings, includes the following steps,
A. it takes frond to cut into segment, is cultivated after being cut into the sterilized seawer washing of frond of segment, generate spore；
B. the spore suspension that seaweed generates is collected, being concentrated into when being monitored with 10 × object lens of eyepiece 40 there are 10 spores in the visual field
Son is cultivated again；In incubation again, makes to be attached to each other aggregation growth between spore, form spore aggregation；
C. it collects spore aggregation and disperses, the spore aggregation seedling after the dispersion of formation goes to seedling as seedling
It ventilates and cultivates in flask；The seedling of drag is acquired as deposit seedling.
Culture solution is natural sea-water, is stored after filtration sterilization is cooled to room temperature spare.
Embodiment 5
A kind of seaweed spore aggregation method for culturing seedlings, includes the following steps,
A. after the frond grown under natural conditions being used sterilizing seawer washing, in 20-25 DEG C, 100 μm of ol/m2The illumination of/s
Lower culture generates spore；
B. the spore suspension that seaweed generates is collected, being concentrated into when being monitored with 10 × object lens of eyepiece 40 has in the visual field greater than 9
A spore, is cultivated again；The spore suspension of concentration is put into illumination constant incubator and is cultivated, illumination constant incubator temperature
Control is at 25 DEG C, and the photoperiod controls in 12L:12D, and intensity control is in 100 μm of ol/m2/ s is cultivated 2.5 days；It was cultivating again
Magnetic stirrer is used in journey, is made to be attached to each other aggregation growth between spore, is formed spore aggregation；
C. it collects spore aggregation and disperses, the spore aggregation seedling after the dispersion of formation goes to seedling as seedling
It ventilates in flask culture, when culture, seedling is put into illumination constant incubator and is cultivated, the control of illumination constant incubator temperature exists
25 DEG C, the photoperiod controls in 12L:12D, and intensity control is in 100 μm of ol/m2/ s is cultivated 2.5 days；
The seedling of drag is acquired as deposit seedling；Deposit seedling, which is put into illumination constant incubator, to be cultivated, illumination constant temperature
The control of incubator temperature is at 10-15 DEG C, and the photoperiod controls in 12L:12D, and intensity control is in 10 μm of ol/m2/ s is cultivated 2 days, storage
It deposits spare.
Embodiment 6
A kind of seaweed spore aggregation method for culturing seedlings, includes the following steps,
A. after frond being used sterilizing seawer washing, in 20-25 DEG C, 100 μm of ol/m2It is cultivated under the illumination of/s, generates spore；
B. the spore suspension that seaweed generates is collected, being concentrated into when being monitored with 10 × object lens of eyepiece 40 has in the visual field greater than 9
A spore, is cultivated again；The spore suspension of concentration is put into illumination constant incubator and is cultivated, illumination constant incubator temperature
Control is at 25 DEG C, and the photoperiod controls in 12L:12D, and intensity control is in 100 μm of ol/m2/ s is cultivated 2.5 days；It was cultivating again
Magnetic stirrer is used in journey, is made to be attached to each other aggregation growth between spore, is formed spore aggregation；
C. it collects spore aggregation and disperses, the spore aggregation seedling after the dispersion of formation goes to seedling as seedling
It ventilates in flask culture, when culture, seedling is put into illumination constant incubator and is cultivated, the control of illumination constant incubator temperature exists
25 DEG C, the photoperiod controls in 12L:12D, and intensity control is in 100 μm of ol/m2/ s is cultivated 2.5 days；
The seedling of drag is acquired as deposit seedling；Deposit seedling, which is put into illumination constant incubator, to be cultivated, illumination constant temperature
The control of incubator temperature is at 10-15 DEG C, and the photoperiod controls in 12L:12D, and intensity control is in 10 μm of ol/m2/ s is cultivated 2 days, storage
It deposits spare.
Embodiment 7
After the sterilized seawater of fresh Enteromorpha collected is cleaned, the algae that 10-200 length is about 1-2mm is cut into
Body segment, it is sterilized after seawer washing after be transferred to equipped with 30mL sterilizing seawater glass culture dish in cultivate, setting culture
Condition are as follows: 20 DEG C of temperature, illumination condition: cold white fluorescence lamp, photoperiod control in 12L:12D, 100 μm of ol/m of light intensity2/s.Herein
Frond segment can continue to generate spore a couple of days under condition of culture.Since the spore of generation has the characteristic of light, collect and dense
Contracting spore suspension.Few drops of spore suspensions are added into the culture dish equipped with 20mL culture solution and adjust its final suspension
Concentration is greater than 104A/mL.Culture dish is placed on magnetic stirring apparatus, revolving speed, the training that this culture dish is arranged according to front are adjusted
Feeding condition, which is placed in, to continue to cultivate in illumination box.With this condition, spore is grown with higher density in glass dish bottom
And attachment is carried out between each other and forms aggregation.
These aggregations are scraped off from culture dish bottom with tweezers, it is big to be formed to carry out disperse agglomerations with electric mixer
Measure scattered small seedling.These small seedling are put into the flask equipped with 500mL culture solution culture of ventilating, other conditions are same as
Front condition of culture.The drag seedling of artificial cultivation of the seedling a part to grow fine to the later period, another part makees
To lay in seedling.Seedling will be laid in and cultivated to 20cm or more and make the source that duration supplies vegetative propagation seedling.To growing way under this condition
Good frond is sheared to obtain spore, and processing method is with reference to above-mentioned.This cultivating process can be repeated several times with
Obtain the spore ball of a large amount of vegetative propagations.
The condition of culture of spore ball is set are as follows: 12 DEG C of temperature, illumination condition: cold white fluorescence lamp, photoperiod control in 12L:
12D, 10 μm of ol/m of light intensity2/s.This condition of culture can make these small seedling survivals 1 year or more and not observe that it is given birth to
Long phenomenon.
In short, the foregoing is merely presently preferred embodiments of the present invention, it is all according to equalization made by scope of the present invention patent
Variation and modification, shall all be covered by the patent of the invention.
Claims (3)
1. a kind of seaweed spore aggregation method for culturing seedlings, it is characterised in that: include the following steps,
A. it takes frond to cut into segment, is cultivated after being cut into the sterilized seawer washing of frond of segment, generate spore；
Or by frond with sterilizing seawer washing after, in 20-25 DEG C, 100 μm of ol/m2It is cultivated under the illumination of/s, generates spore；
In step A, it is cut into after the sterilized seawer washing of frond of segment to be put into illumination constant incubator and cultivates, the training of illumination constant temperature
The control of box temperature degree is supported at 18-25 DEG C, the photoperiod controls in 12L:12D, and intensity control is in 100 μm of ol/m2/ s is cultivated 2-5 days, is produced
Raw spore；
B. the spore suspension that seaweed generates is collected, being concentrated into when being monitored with 10 × object lens of eyepiece 40 there are greater than 6 spores in the visual field
Son is cultivated again；It is stirred in incubation again, makes to be attached to each other aggregation growth between spore, form spore aggregation；
In step B, when cultivating again, the spore suspension of concentration is put into illumination constant incubator and is cultivated, the training of illumination constant temperature
The control of box temperature degree is supported at 18-25 DEG C, the photoperiod controls in 12L:12D, and intensity control is in 100 μm of ol/m2/ s is cultivated 2-3 days；
C. it collects spore aggregation and disperses, seedling is gone to flask as seedling by the spore aggregation seedling after the dispersion of formation
Middle ventilation culture acquires the seedling of drag as deposit seedling；
In step C, when culture, seedling is put into illumination constant incubator and is cultivated, illumination constant incubator temperature is controlled in 18-
25 DEG C, the photoperiod controls in 12L:12D, and intensity control is in 100 μm of ol/m2/ s is cultivated 2-3 days；
In step C, deposit seedling is put into illumination constant incubator and cultivates, and the control of illumination constant incubator temperature is at 10-15 DEG C, light
Period controls in 12L:12D, and intensity control is in 10 μm of ol/m2/ s is cultivated 2 days, is stored for future use.
2. seaweed spore aggregation method for culturing seedlings according to claim 1, it is characterised in that: in step A, take nature
The frond of lower growth.
3. seaweed spore aggregation method for culturing seedlings according to claim 1, it is characterised in that: in step B, stirred using magnetic force
Mix device stirring.
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101167439A (en) * | 2007-11-16 | 2008-04-30 | 中国海洋大学 | Polysiphonia urceolata tetraspore seedling formation method |
| CN101176420A (en) * | 2007-11-07 | 2008-05-14 | 孙庆海 | Cuprum algae artificial raise seedling technique |
| CN101613673A (en) * | 2009-07-28 | 2009-12-30 | 中国科学院南海海洋研究所 | A kind of collection of enteromorpha linza spore and cultural method |
| CN101692787A (en) * | 2009-09-01 | 2010-04-14 | 中国科学院南海海洋研究所 | Method for collecting and culturing ulva spores |
| CN101827517A (en) * | 2007-08-01 | 2010-09-08 | 国立大学法人北海道大学 | Method of producing sporophytes of plant belonging to the order laminariales and base material to be used therein |
| CN103392585A (en) * | 2013-07-15 | 2013-11-20 | 中国水产科学研究院黄海水产研究所 | New method for culturing kelp seedlings in scale without substratum |
-
2014
- 2014-12-25 CN CN201410822596.3A patent/CN105766610B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101827517A (en) * | 2007-08-01 | 2010-09-08 | 国立大学法人北海道大学 | Method of producing sporophytes of plant belonging to the order laminariales and base material to be used therein |
| CN101176420A (en) * | 2007-11-07 | 2008-05-14 | 孙庆海 | Cuprum algae artificial raise seedling technique |
| CN101167439A (en) * | 2007-11-16 | 2008-04-30 | 中国海洋大学 | Polysiphonia urceolata tetraspore seedling formation method |
| CN101613673A (en) * | 2009-07-28 | 2009-12-30 | 中国科学院南海海洋研究所 | A kind of collection of enteromorpha linza spore and cultural method |
| CN101692787A (en) * | 2009-09-01 | 2010-04-14 | 中国科学院南海海洋研究所 | Method for collecting and culturing ulva spores |
| CN103392585A (en) * | 2013-07-15 | 2013-11-20 | 中国水产科学研究院黄海水产研究所 | New method for culturing kelp seedlings in scale without substratum |
Non-Patent Citations (1)
| Title |
|---|
| 浒苔规模化人工育苗技术研究;崔建军等;《上海海洋大学学报》;20140917;第23卷(第5期);第697-704页 |
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