CN105766610A - Spore aggregate seedling raising method for seaweed - Google Patents
Spore aggregate seedling raising method for seaweed Download PDFInfo
- Publication number
- CN105766610A CN105766610A CN201410822596.3A CN201410822596A CN105766610A CN 105766610 A CN105766610 A CN 105766610A CN 201410822596 A CN201410822596 A CN 201410822596A CN 105766610 A CN105766610 A CN 105766610A
- Authority
- CN
- China
- Prior art keywords
- spore
- seedling
- constant incubator
- sargassum
- culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
Landscapes
- Cultivation Of Seaweed (AREA)
Abstract
The invention relates to the technical field of biology and discloses a spore aggregate seedling raising method for seaweed. According to the method, through carrying out external stimulus, such as cutting-off or high-temperature glare on the seaweed growing in a natural state, a large amount of spores can be generated simultaneously at a high speed. A generated spore suspension is collected, is concentration and then is subjected to culture under appropriate conditions. Under the culture conditions, the spores grow at the bottom of a glass dish at relatively high density and are aggregated to one another so as to form spore aggregates. One part of the spore aggregates are dispersed and then subjected to aerated re-culture so as to be developed into the seaweed, and the other part of the spore aggregates are subjected to low-temperature and low-light-intensity culture so as to preserve seeds. The provided method can provide a technical base for artificial formalized seaweed culture. The method has the major advantages that the spore aggregates grow in a nutrient solution in a suspended aggregation manner; the disadvantage in the traditional seedling raising that attachments and growth places are required to be provided for seaweed spore or zygotes and the like is avoided; and furthermore, the dispersed aggregates can be used for providing a guarantee of adjustable seedling quantity in subsequent further amplified culture.
Description
Technical field
The present invention relates to biological technical field, particularly relate to a kind of Sargassum spore aggregation method for culturing seedlings.
Background technology
China marine site is vast, and Sargassum is resource important in ocean, and the many kinds in red algae, chlorella and Brown algae have at a relatively high economic worth.But in recent years, along with the exploitation to marine algae, the demand of algae increases day by day, the naturally wild edible Sargassum in sea area can not meet far away the demand of market volume and quality.Therefore expand and carry out the important content that algal cultivation process technology is coastal state.
Artificial culture Sargassum is mainly Thallus Laminariae (Thallus Eckloniae), Undaria Pinnatifida, Thallus Porphyrae, Gracilaria tenuistipitata, Eucheuma muricatum (Gmel.) Web.Van Bos. and Monostroma nitidum Wittr. etc. both at home and abroad at present, and major part mainly takes traditional algal cultivation mode.Traditional algal cultivation mode is to utilize the length former frond more than 10cm naturally to sprout at 20-30 DEG C to produce asexual spore or female and male gametophyte.Frond upper part after release starts the dead putrefactive phenomenon of decline gradually occur.Owing to newly generated spore is less, there is water body, tide, season, water pollution, temperature, salinity altercation etc. plus nature mariculture area simultaneously and affect the uncontrollable factor of spore survival rate, constrain new sporogenic growth promoter, the one-tenth algae that can not stably produce to be available in a large number gathering can be caused.
Although currently mainly economical alga has achieved artificial culture in various degree, but the stability of its yield and quality need further raising.Domestic at present not yet have the correlation technique utilizing Sargassum spore aggregation suspension growth to form seedling breeding; therefore carrying out the application in Sargassum nursery of the spore glomeration method necessary with the method obtaining the cultivation of Sargassum seedling, the method can provide technical foundation for artificial large-scale cultivation Sargassum.
Summary of the invention
The present invention is directed to existing shortcoming, disclose a kind of Sargassum spore aggregation method for culturing seedlings.
In order to solve above-mentioned technical problem, the present invention is addressed by following technical proposals.
A kind of Sargassum spore aggregation method for culturing seedlings, comprises the following steps,
A. take frond and cut into fragment, cultivate after being cut into the sterilized seawer washing of frond of fragment, produce spore；Or by frond with after sterilizing seawer washing, at 20-25 DEG C, 100 μm ol/m2Cultivate under the illumination of/s, produce spore；Spore refers to asexual spore, female and male gametophyte.
B. collecting the spore suspension that Sargassum produces, being concentrated into when monitoring with eyepiece 10 × object lens 40 has in the visual field more than 6 spores, again cultivates；Incubation again stirs, makes attachment gathering growth mutually between spore, form spore aggregation；Incubation again stirs, makes between spore, mutually to adhere to gathering growth with higher density and form spore aggregation.
C. collecting spore aggregation and disperse, seedling, as seedling, is gone to aerobic culture in flask by the spore aggregation Seedling after the dispersion of formation, gathers the seedling of drag as deposit Seedling.A spore part after dispersion can be further cultured for growth for becoming algae through ventilation, and another part is standby as deposit Seedling；This condition of culture can make these spore balls at least survive more than 1 year and not observe that it grows.When seedling goes to aerobic culture in flask, can there is suspension growth phenomenon in part seedling.Spore number in spore aggregation after dispersion is controlled by the present invention, and the present invention is suspended in culture fluid to grow, and what be different from traditional method is attached to wire side or rope growth, thus avoiding the phenomenon that the yield caused because of difficulty of gathering reduces.
As preferably, in step A, taking the frond of growth under naturalness.
As preferably, in step A, putting in illumination constant incubator and cultivate after being cut into the sterilized seawer washing of frond of fragment, illumination constant incubator temperature controls at 18-25 DEG C, and the photoperiod controls at 12L:12D, and intensity control is at 100 μm of ol/m2/ s, cultivates 2-5 days, produces spore.
As preferably, in step B, when again cultivating, being put into by the spore suspension of concentration in illumination constant incubator and cultivate, illumination constant incubator temperature controls at 18-25 DEG C, and the photoperiod controls at 12L:12D, and intensity control is at 100 μm of ol/m2/ s, cultivates 2-3 days.
As preferably, in step B, using magnetic stirrer.
As preferably, in step C, during cultivation, being put into by seedling in illumination constant incubator and cultivate, illumination constant incubator temperature controls at 18-25 DEG C, and the photoperiod controls at 12L:12D, and intensity control is at 100 μm of ol/m2/ s, cultivates 2-3 days.
As preferably, in step C, deposit Seedling is put in illumination constant incubator and is cultivated, and illumination constant incubator temperature controls at 10-15 DEG C, and the photoperiod controls at 12L:12D, and intensity control is at 10 μm of ol/m2/ s, cultivates 3 days, stores for future use.Deposit Seedling after process can supply the source of seedling as persistence.Deposit Seedling is put in illumination constant incubator and is cultivated, and illumination constant incubator temperature controls at 10-15 DEG C, and the photoperiod controls at 12L:12D, and intensity control is at 10 μm of ol/m2/ s, this condition of culture can make these seedling survival more than 1 year, and does not observe that it grows.
Compared with prior art, the invention have the benefit that
(1) " the spore aggregation " that the present invention adopts is as the seedling of Sargassum artificial culture initial stage.It has a major advantage in that and makes spore aggregation grow in culture fluid with suspension clustered pattern, being different from tradition nursery needs Sargassum spore or zygote etc. provide attachment provide growth place, and additionally this step of disperse agglomerations can provide for controllable seedling numbers in follow-up further amplification culture and ensure.
(2) cultural method of the present invention gathers spore, easy and simple to handle, cycle is short, practical, and a large amount of spore of lasting collection can be stablized, it is different from traditional seedling raising manners and frond Maturity is required harshness, solve in traditional approach because ripe frond discharges spore and the dead technical problem that fails.
(3) so what time the superiority of spore clumps nursery also has: first, is beneficial to preservation, and the seedling that spore glomeration method is formed can preserve for a long time.Second, the seedling of formation is all clone body, can be easy to whenever carry out produce or test compareing.3rd, the spore obtained by repeatedly phototaxis can avoid the pollution of other algae, forms safe and reliable seed.4th, survival rate is high, it is to avoid the factors such as extraneous factor is natural enemy such as, environment conversion affect.
Detailed description of the invention
Embodiment 1
A kind of Sargassum spore aggregation method for culturing seedlings, comprises the following steps,
A. take the frond of growth under naturalness and cut into fragment, after being cut into the sterilized seawer washing of frond of fragment, putting in illumination constant incubator and cultivate, illumination constant incubator temperature controls at 20 DEG C, photoperiod controls at 12L:12D, and intensity control is at 100 μm of ol/m2/ s, cultivates 2 days, produces spore；
B. collecting the spore suspension that Sargassum produces, being concentrated into when monitoring with eyepiece 10 × object lens 40 has in the visual field more than 6 spores, again cultivates；Being put into by the spore suspension of concentration in illumination constant incubator and cultivate, illumination constant incubator temperature controls at 20 DEG C, and the photoperiod controls at 12L:12D, and intensity control is at 100 μm of ol/m2/ s, cultivates 2 days；Incubation again uses magnetic stirrer, makes attachment gathering growth mutually between spore, form spore aggregation；
C. collect spore aggregation and disperse, spore aggregation Seedling after the dispersion formed is as seedling, seedling is gone to aerobic culture in flask, during cultivation, seedling is put in illumination constant incubator and cultivate, illumination constant incubator temperature controls at 20 DEG C, and the photoperiod controls at 12L:12D, and intensity control is at 100 μm of ol/m2/ s, cultivates 2 days；Gather the seedling of drag as deposit Seedling；Deposit Seedling is put in illumination constant incubator and is cultivated, and illumination constant incubator temperature controls at 10-15 DEG C, and the photoperiod controls at 12L:12D, and intensity control is at 10 μm of ol/m2/ s, cultivates 2 days, stores for future use.
Embodiment 2
A kind of Sargassum spore aggregation method for culturing seedlings, comprises the following steps,
A. taking frond and cut into fragment, after being cut into the sterilized seawer washing of frond of fragment, put in illumination constant incubator and cultivate, illumination constant incubator temperature controls at 18 DEG C, and the photoperiod controls at 12L:12D, and intensity control is at 100 μm of ol/m2/ s, cultivates 5 days, produces spore；
B. collecting the spore suspension that Sargassum produces, being concentrated into when monitoring with eyepiece 10 × object lens 40 has in the visual field more than 6 spores, again cultivates；Being put into by the spore suspension of concentration in illumination constant incubator and cultivate, illumination constant incubator temperature controls at 18 DEG C, and the photoperiod controls at 12L:12D, and intensity control is at 100 μm of ol/m2/ s, cultivates 3 days；Incubation again uses magnetic stirrer, makes attachment gathering growth mutually between spore, form spore aggregation；
C. collect spore aggregation and disperse, spore aggregation Seedling after the dispersion formed is as seedling, seedling is gone to aerobic culture in flask, during cultivation, seedling is put in illumination constant incubator and cultivate, illumination constant incubator temperature controls at 18 DEG C, and the photoperiod controls at 12L:12D, and intensity control is at 100 μm of ol/m2/ s, cultivates 3 days；
Gather the seedling of drag as deposit Seedling；Deposit Seedling is put in illumination constant incubator and is cultivated, and illumination constant incubator temperature controls at 10 DEG C, and the photoperiod controls at 12L:12D, and intensity control is at 10 μm of ol/m2/ s, cultivates 2 days, stores for future use.
Embodiment 3
A kind of Sargassum spore aggregation method for culturing seedlings, comprises the following steps,
A. take the frond of growth under naturalness and cut into fragment, after being cut into the sterilized seawer washing of frond of fragment, putting in illumination constant incubator and cultivate, illumination constant incubator temperature controls at 25 DEG C, photoperiod controls at 12L:12D, and intensity control is at 100 μm of ol/m2/ s, cultivates 3 days, produces spore；
B. collecting the spore suspension that Sargassum produces, being concentrated into when monitoring with eyepiece 10 × object lens 40 has in the visual field more than 9 spores, again cultivates；Being put into by the spore suspension of concentration in illumination constant incubator and cultivate, illumination constant incubator temperature controls at 25 DEG C, and the photoperiod controls at 12L:12D, and intensity control is at 100 μm of ol/m2/ s, cultivates 2.5 days；Incubation again uses magnetic stirrer, makes attachment gathering growth mutually between spore, form spore aggregation；
C. collect spore aggregation and disperse, spore aggregation Seedling after the dispersion formed is as seedling, seedling is gone to aerobic culture in flask, during cultivation, seedling is put in illumination constant incubator and cultivate, illumination constant incubator temperature controls at 25 DEG C, and the photoperiod controls at 12L:12D, and intensity control is at 100 μm of ol/m2/ s, cultivates 2.5 days；
Gather the seedling of drag as deposit Seedling；Deposit Seedling is put in illumination constant incubator and is cultivated, and illumination constant incubator temperature controls at 10-15 DEG C, and the photoperiod controls at 12L:12D, and intensity control is at 10 μm of ol/m2/ s, cultivates 2 days, stores for future use.
Embodiment 4
A kind of Sargassum spore aggregation method for culturing seedlings, comprises the following steps,
A. take frond and cut into fragment, cultivate after being cut into the sterilized seawer washing of frond of fragment, produce spore；
B. collecting the spore suspension that Sargassum produces, being concentrated into when monitoring with eyepiece 10 × object lens 40 has 10 spores in the visual field, again cultivate；In incubation again, make attachment gathering growth mutually between spore, form spore aggregation；
C. collecting spore aggregation and disperse, seedling, as seedling, is gone to aerobic culture in flask by the spore aggregation Seedling after the dispersion of formation；Gather the seedling of drag as deposit Seedling.
Culture fluid is natural sea-water, is cooled to after room temperature to store standby through filtration sterilization.
Embodiment 5
A kind of Sargassum spore aggregation method for culturing seedlings, comprises the following steps,
A. by after the frond sterilizing seawer washing that grows under naturalness, at 20-25 DEG C, 100 μm ol/m2Cultivate under the illumination of/s, produce spore；
B. collecting the spore suspension that Sargassum produces, being concentrated into when monitoring with eyepiece 10 × object lens 40 has in the visual field more than 9 spores, again cultivates；Being put into by the spore suspension of concentration in illumination constant incubator and cultivate, illumination constant incubator temperature controls at 25 DEG C, and the photoperiod controls at 12L:12D, and intensity control is at 100 μm of ol/m2/ s, cultivates 2.5 days；Incubation again uses magnetic stirrer, makes attachment gathering growth mutually between spore, form spore aggregation；
C. collect spore aggregation and disperse, spore aggregation Seedling after the dispersion formed is as seedling, seedling is gone to aerobic culture in flask, during cultivation, seedling is put in illumination constant incubator and cultivate, illumination constant incubator temperature controls at 25 DEG C, and the photoperiod controls at 12L:12D, and intensity control is at 100 μm of ol/m2/ s, cultivates 2.5 days；
Gather the seedling of drag as deposit Seedling；Deposit Seedling is put in illumination constant incubator and is cultivated, and illumination constant incubator temperature controls at 10-15 DEG C, and the photoperiod controls at 12L:12D, and intensity control is at 10 μm of ol/m2/ s, cultivates 2 days, stores for future use.
Embodiment 6
A kind of Sargassum spore aggregation method for culturing seedlings, comprises the following steps,
A. by frond with after sterilizing seawer washing, at 20-25 DEG C, 100 μm ol/m2Cultivate under the illumination of/s, produce spore；
B. collecting the spore suspension that Sargassum produces, being concentrated into when monitoring with eyepiece 10 × object lens 40 has in the visual field more than 9 spores, again cultivates；Being put into by the spore suspension of concentration in illumination constant incubator and cultivate, illumination constant incubator temperature controls at 25 DEG C, and the photoperiod controls at 12L:12D, and intensity control is at 100 μm of ol/m2/ s, cultivates 2.5 days；Incubation again uses magnetic stirrer, makes attachment gathering growth mutually between spore, form spore aggregation；
C. collect spore aggregation and disperse, spore aggregation Seedling after the dispersion formed is as seedling, seedling is gone to aerobic culture in flask, during cultivation, seedling is put in illumination constant incubator and cultivate, illumination constant incubator temperature controls at 25 DEG C, and the photoperiod controls at 12L:12D, and intensity control is at 100 μm of ol/m2/ s, cultivates 2.5 days；
Gather the seedling of drag as deposit Seedling；Deposit Seedling is put in illumination constant incubator and is cultivated, and illumination constant incubator temperature controls at 10-15 DEG C, and the photoperiod controls at 12L:12D, and intensity control is at 10 μm of ol/m2/ s, cultivates 2 days, stores for future use.
Embodiment 7
After the sterilized sea water of fresh Entermorpha collected is cleaned, cut into the frond fragment that 10-200 length is about 1-2mm, it is transferred in the glass culture dish equipped with 30mL sterilizing sea water after seawer washing after sterilized and cultivates, arranging condition of culture is: temperature 20 DEG C, illumination condition: cool white fluorescent lamp, photoperiod controls at 12L:12D, 100 μm of ol/m of light intensity2/s.Under this condition of culture, frond fragment can continue to produce spore a couple of days.Spore owing to producing has the characteristic of light, collects and concentrates spore suspension.In the culture dish equipped with 20mL culture fluid, add several spore suspension and regulate its final suspension concentration more than 104Individual/mL.Culture dish is placed on magnetic stirring apparatus, adjusts rotating speed, this culture dish is placed in illumination box according to the condition of culture above arranged and continues to cultivate.With this understanding, spore carries out growing and carry out each other attachment bottom glass dish with higher density and forms aggregation.
With tweezers, these aggregations are scraped off bottom culture dish, carry out disperse agglomerations to form a large amount of scattered little seedling with motor stirrer.These little seedling are put into aerobic culture in the flask equipped with 500mL culture fluid, and other conditions are same as above condition of culture.The seedling part grown fine is in order to the artificial culture in later stage, and the drag seedling of another part is as deposit Seedling.Deposit Seedling cultivation is made to more than 20cm the source of persistence supply asexual propagation seedling.Shearing to obtain spore to the frond grown fine under this condition, processing method is with reference to above-mentioned.Can repeatedly repeat this cultivating process to obtain a large amount of vegetative spore ball.
The condition of culture arranging spore ball is: temperature 12 DEG C, illumination condition: cool white fluorescent lamp, and the photoperiod controls at 12L:12D, 10 μm of ol/m of light intensity2/s.This condition of culture can make these little seedling survival more than 1 year and not observe that it the phenomenon of growth occurs.
In a word, the foregoing is only presently preferred embodiments of the present invention, all equalizations made according to the present patent application the scope of the claims change and modify, and all should belong to the covering scope of patent of the present invention.
Claims (7)
1. a Sargassum spore aggregation method for culturing seedlings, it is characterised in that: comprise the following steps,
A. take frond and cut into fragment, cultivate after being cut into the sterilized seawer washing of frond of fragment, produce spore；
Or by frond with after sterilizing seawer washing, at 20-25 DEG C, 100 μm ol/m2Cultivate under the illumination of/s, produce spore；
B. collecting the spore suspension that Sargassum produces, being concentrated into when monitoring with eyepiece 10 × object lens 40 has in the visual field more than 6 spores, again cultivates；Incubation again stirs, makes attachment gathering growth mutually between spore, form spore aggregation；
C. collecting spore aggregation and disperse, seedling, as seedling, is gone to aerobic culture in flask by the spore aggregation Seedling after the dispersion of formation, gathers the seedling of drag as deposit Seedling.
2. Sargassum spore aggregation method for culturing seedlings according to claim 1, it is characterised in that: in step A, take the frond of growth under naturalness.
3. Sargassum spore aggregation method for culturing seedlings according to claim 1, it is characterized in that: in step A, put into after being cut into the sterilized seawer washing of frond of fragment in illumination constant incubator and cultivate, illumination constant incubator temperature controls at 18-25 DEG C, photoperiod controls at 12L:12D, and intensity control is at 100 μm of ol/m2/ s, cultivates 2-5 days, produces spore.
4. Sargassum spore aggregation method for culturing seedlings according to claim 3, it is characterized in that: in step B, when again cultivating, the spore suspension of concentration is put in illumination constant incubator and cultivate, illumination constant incubator temperature controls at 18-25 DEG C, photoperiod controls at 12L:12D, and intensity control is at 100 μm of ol/m2/ s, cultivates 2-3 days.
5. Sargassum spore aggregation method for culturing seedlings according to claim 1, it is characterised in that: in step B, use magnetic stirrer.
6. Sargassum spore aggregation method for culturing seedlings according to claim 1, it is characterized in that: in step C, during cultivation, seedling is put in illumination constant incubator and cultivate, illumination constant incubator temperature controls at 18-25 DEG C, photoperiod controls at 12L:12D, and intensity control, at 100 μm of ol/m2/s, is cultivated 2-3 days.
7. Sargassum spore aggregation method for culturing seedlings according to claim 1, it is characterized in that: in step C, deposit Seedling is put in illumination constant incubator and is cultivated, illumination constant incubator temperature controls at 10-15 DEG C, photoperiod controls at 12L:12D, intensity control, at 10 μm of ol/m2/s, is cultivated 2 days, is stored for future use.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410822596.3A CN105766610B (en) | 2014-12-25 | 2014-12-25 | A kind of seaweed spore aggregation method for culturing seedlings |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410822596.3A CN105766610B (en) | 2014-12-25 | 2014-12-25 | A kind of seaweed spore aggregation method for culturing seedlings |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105766610A true CN105766610A (en) | 2016-07-20 |
| CN105766610B CN105766610B (en) | 2019-05-14 |
Family
ID=56388490
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410822596.3A Active CN105766610B (en) | 2014-12-25 | 2014-12-25 | A kind of seaweed spore aggregation method for culturing seedlings |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105766610B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112042524A (en) * | 2020-09-09 | 2020-12-08 | 扬州大学 | Method for rapidly and continuously culturing enteromorpha |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101167439A (en) * | 2007-11-16 | 2008-04-30 | 中国海洋大学 | Polysiphonia urceolata tetraspore seedling formation method |
| CN101176420A (en) * | 2007-11-07 | 2008-05-14 | 孙庆海 | Cuprum algae artificial raise seedling technique |
| CN101613673A (en) * | 2009-07-28 | 2009-12-30 | 中国科学院南海海洋研究所 | A kind of collection of enteromorpha linza spore and cultural method |
| CN101692787A (en) * | 2009-09-01 | 2010-04-14 | 中国科学院南海海洋研究所 | Method for collecting and culturing ulva spores |
| CN101827517A (en) * | 2007-08-01 | 2010-09-08 | 国立大学法人北海道大学 | Method of producing sporophytes of plant belonging to the order laminariales and base material to be used therein |
| CN103392585A (en) * | 2013-07-15 | 2013-11-20 | 中国水产科学研究院黄海水产研究所 | New method for culturing kelp seedlings in scale without substratum |
-
2014
- 2014-12-25 CN CN201410822596.3A patent/CN105766610B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101827517A (en) * | 2007-08-01 | 2010-09-08 | 国立大学法人北海道大学 | Method of producing sporophytes of plant belonging to the order laminariales and base material to be used therein |
| CN101176420A (en) * | 2007-11-07 | 2008-05-14 | 孙庆海 | Cuprum algae artificial raise seedling technique |
| CN101167439A (en) * | 2007-11-16 | 2008-04-30 | 中国海洋大学 | Polysiphonia urceolata tetraspore seedling formation method |
| CN101613673A (en) * | 2009-07-28 | 2009-12-30 | 中国科学院南海海洋研究所 | A kind of collection of enteromorpha linza spore and cultural method |
| CN101692787A (en) * | 2009-09-01 | 2010-04-14 | 中国科学院南海海洋研究所 | Method for collecting and culturing ulva spores |
| CN103392585A (en) * | 2013-07-15 | 2013-11-20 | 中国水产科学研究院黄海水产研究所 | New method for culturing kelp seedlings in scale without substratum |
Non-Patent Citations (1)
| Title |
|---|
| 崔建军等: "浒苔规模化人工育苗技术研究", 《上海海洋大学学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112042524A (en) * | 2020-09-09 | 2020-12-08 | 扬州大学 | Method for rapidly and continuously culturing enteromorpha |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105766610B (en) | 2019-05-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102150624B (en) | Tissue culture and rapid propagation method for pinellia tuber plant | |
| CN103858745B (en) | Tawny daylily algae artificial seedling rearing technology | |
| CN101595824B (en) | Rapid in-vitro seedling raising method by utilizing sandalwood seed embryo | |
| CN101720670A (en) | Rapid breeding method for pinellia tuber tissue culture | |
| CN101773072B (en) | Method for culturing isolated microspore of common head cabbage to obtain regeneration plant | |
| KR101040240B1 (en) | Method for mass production of micro potato by bioreactor culture | |
| CN104542237B (en) | Seedling-raising method for gracilaria algae | |
| CN107494277A (en) | A kind of gingko tissue culture and rapid propagation method | |
| CN103070078A (en) | Rapid propagation method for performing tissue culture by using taro stem tip | |
| CN107494275A (en) | A kind of smilax tissue culture and rapid propagation method | |
| CN101422127A (en) | Gulf-weed sexual propagating and seedling rearing method | |
| CN101120663A (en) | Artificial cultivating method for pearl | |
| CN101717748B (en) | Method for generating macrogametocyte by somatic cell of inducing undaria pinnatifida parthenogenesis for juvenile sporophyte | |
| CN1973613A (en) | Temperature controlling low-salinity sargassum thunbergii seedling growing process | |
| CN101606468B (en) | Simple method for cultivating nostoc commune and cultivating device | |
| CN109757377A (en) | A kind of cultural method for accelerating fritillaria thunbergii reproduction speed | |
| CN105766610A (en) | Spore aggregate seedling raising method for seaweed | |
| CN112790108B (en) | Tissue culture and field planting method of gelidium amansii | |
| CN104041405B (en) | A kind of method of rapid screening high yield sea-tangle strain | |
| CN107155882A (en) | A kind of medicinal bletilla striata aseptic seeding fast seedling-cultivating method | |
| CN103250643B (en) | Tangut white spine clone in-vitro rooting culture method | |
| CN107494261B (en) | A kind of cultural method for establishing cabbage heart suspension cell line | |
| CN107568063B (en) | A kind of oilseed plant Asiatic sweet leaf tissue culture and rapid propagation method | |
| CN105660416B (en) | The method of China fir test tube seedling root induction | |
| CN103461090B (en) | Filament seedling and mariculture method of grateloupia filicina |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20230224 Address after: 315702 Huang Biao Xiang Gao Ni Cun, Xiangshan County, Ningbo City, Zhejiang Province Patentee after: Xiangshan Xuwen Algae Development Co.,Ltd. Address before: Room B1172, 1st floor, Building 1 (North), No. 368, Liuhe Road, Binjiang District, Hangzhou City, Zhejiang Province, 310000 Patentee before: Zhu Wenrong |
|
| TR01 | Transfer of patent right |