CN108244009A - In-vitro culture method in a kind of Eriocheir sinensis embryo room - Google Patents
In-vitro culture method in a kind of Eriocheir sinensis embryo room Download PDFInfo
- Publication number
- CN108244009A CN108244009A CN201810105425.7A CN201810105425A CN108244009A CN 108244009 A CN108244009 A CN 108244009A CN 201810105425 A CN201810105425 A CN 201810105425A CN 108244009 A CN108244009 A CN 108244009A
- Authority
- CN
- China
- Prior art keywords
- eriocheir sinensis
- water
- vitro
- culture method
- vitro culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000371997 Eriocheir sinensis Species 0.000 title claims abstract description 31
- 238000000338 in vitro Methods 0.000 title claims abstract description 23
- 210000001161 mammalian embryo Anatomy 0.000 title claims abstract description 19
- 238000012136 culture method Methods 0.000 title claims abstract description 12
- 235000013601 eggs Nutrition 0.000 claims abstract description 14
- 241001131796 Botaurus stellaris Species 0.000 claims abstract description 5
- 238000010790 dilution Methods 0.000 claims abstract description 5
- 239000012895 dilution Substances 0.000 claims abstract description 5
- 238000005273 aeration Methods 0.000 claims abstract description 4
- 239000008399 tap water Substances 0.000 claims abstract description 4
- 235000020679 tap water Nutrition 0.000 claims abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 31
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims description 9
- 210000004681 ovum Anatomy 0.000 claims description 6
- 102000002322 Egg Proteins Human genes 0.000 claims description 4
- 108010000912 Egg Proteins Proteins 0.000 claims description 4
- 238000011534 incubation Methods 0.000 claims description 4
- 238000011161 development Methods 0.000 claims description 3
- 238000006213 oxygenation reaction Methods 0.000 claims description 2
- 230000001954 sterilising effect Effects 0.000 claims description 2
- 238000004659 sterilization and disinfection Methods 0.000 claims description 2
- 238000003756 stirring Methods 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 6
- 238000011160 research Methods 0.000 abstract description 5
- 230000013020 embryo development Effects 0.000 abstract description 4
- 230000004083 survival effect Effects 0.000 abstract description 4
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000034004 oogenesis Effects 0.000 description 2
- 241000238424 Crustacea Species 0.000 description 1
- 230000003187 abdominal effect Effects 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 238000004061 bleaching Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 210000002257 embryonic structure Anatomy 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000012447 hatching Effects 0.000 description 1
- 230000000366 juvenile effect Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000012549 training Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/59—Culture of aquatic animals of shellfish of crustaceans, e.g. lobsters or shrimps
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/17—Hatching, e.g. incubators
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The present invention relates to in-vitro culture method in a kind of Eriocheir sinensis embryo room, including：(1) the Eriocheir sinensis ovigerous crab of fishing is transported to behind interior and is used to cultivate with the tap water dilution bittern after aeration；(2) Eriocheir sinensis ovigerous crab is selected, fertilized eggs are removed into culture dish and carry out cultured in vitro, you can.With the present invention, cultured in vitro survival rate reaches more than 90% in Eriocheir sinensis embryo room, and can embryonic development be observed or be sampled in real time, this is of great significance for carrying out Eriocheir sinensis Seedling production and scientific research.
Description
Technical field
The invention belongs to aquaculture field, in-vitro culture method in more particularly to a kind of Eriocheir sinensis embryo room.
Background technology
Eriocheir sinensis occupies important economy as the important economic kind of crustacean in China's culture fishery
Status.In the actual production and artificial breeding of Eriocheir sinensis, the embryo of Eriocheir sinensis fertilized eggs is typically in parent
Hatching is developed on abdominal appendage, so need to raise ovigerous crab during artificial incubation meticulously, science regulating water quality, special messenger is responsible for, this is straight
Connect the production cost for increasing parent crab.If by Eriocheir sinensis fertilized eggs cultured in vitro, the raising pipe of ovigerous crab can be reduced
Cost is managed, low cost is realized, efficiently beneficially cultivates juvenile crab.In addition, routine observation oogenesis is needed in actual production or scientific research
Crab embryonic development situation obtains different times embryo and is used as research material etc., and therefore, urgent need establishes a kind of Eriocheir sinensis embryo
Indoor in-vitro culture method.
Invention content
The technical problems to be solved by the invention are to provide in-vitro culture method in a kind of Eriocheir sinensis embryo room, use
This method, cultured in vitro survival rate reaches more than 90% in Eriocheir sinensis embryo room, and can in real time to embryonic development into
Row observation or sampling, this is of great significance for carrying out Eriocheir sinensis Seedling production and scientific research.
The present invention provides in-vitro culture method in a kind of Eriocheir sinensis embryo room, including：
(1) it after the Eriocheir sinensis ovigerous crab of fishing being transported to interior, is used to support with the tap water dilution bittern after aeration
It grows, the salinity of breeding water is 18~19, and 18~20 DEG C of water temperature keeps 30~40cm of the depth of water；
(2) Eriocheir sinensis ovigerous crab is selected, fertilized eggs are removed into culture dish and carry out cultured in vitro：In fertilized eggs
During cultured in vitro, 9 points of every morning and afternoon 17:00 point is respectively changed a water, 2/3 left side of each quantity of exchanged water for raw water body
The right side, every morning, noon and afternoon stir fertilized eggs 1 time with suction pipe respectively；It keeps sterilizing, be oxygenated simultaneously, choosing bad ovum in time,
.
Development of fertilized ova in the step (2) is to heart beat period.
The salinity of culture dish in the step (2) is 18~19,18~20 DEG C of water temperature.
Disinfection in the step (2) is specially that the formaldehyde of a concentration of 50ppm is added in water body.
Oxygenation in the step (2) is specially to be oxygenated incessantly for 24 hours in the water body of culture dish.
Advantageous effect
With the present invention, cultured in vitro survival rate reaches more than 90% in Eriocheir sinensis embryo room, and can be real-time
Embryonic development is observed or is sampled, this is of great significance for carrying out Eriocheir sinensis Seedling production and scientific research.
Specific embodiment
With reference to specific embodiment, the present invention is further explained.It should be understood that these embodiments are merely to illustrate the present invention
Rather than it limits the scope of the invention.In addition, it should also be understood that, after reading the content taught by the present invention, people in the art
Member can make various changes or modifications the present invention, and such equivalent forms equally fall within the application the appended claims and limited
Range.
Embodiment 1
The Eriocheir sinensis ovigerous crab manually caught is collected from entrance of Changjiang River, is transported to behind laboratory and temporarily supports in circular sink
In, the size of sink is adjusted according to actual needs, with the tap water dilution bittern after aeration for cultivating ovigerous crab, cultivates
It is 18 with the salinity of water, 18 DEG C of water temperature keeps depth of water 30cm.The bottom of sink place veil, provide its inhabit it is hidden
Place.
More than weight 360g, the Eriocheir sinensis ovigerous crab that oogenesis is more, vigor is good are chosen, will be carefully developed to tweezers
The fertilized eggs of heart beat period are removed into glass culture dish, and a diameter of 10cm of culture dish can adjust size according to actual needs.Often
8 embryos are placed in a culture dish, embryo's quantity can be adjusted according to the size of culture dish.Fertilized eggs are use up with plastics small spoon
Amount, which is scattered, is laid in the bottom of culture dish, avoids agglomerating be packed together.Water in culture dish is also the bittern water after dilution, salt
It is 18 to spend, 18 DEG C of water temperature.
During the cultured in vitro of fertilized eggs, 9 points of every morning and afternoon 17:00 point is respectively changed a water, changes water every time
2/3 or so for raw water body is measured, every morning, noon and afternoon fertilized eggs is stirred 1 time with suction pipe respectively, ovum is prevented to be adhered to training
Support ware bottom.The formaldehyde of a concentration of 50ppm is added in water body, prevents the generation of disease in incubation.In the water of culture dish
It is oxygenated incessantly for 24 hours in body, keeps the abundance of dissolved oxygen in incubation.The development condition of observation fertilized eggs daily, chooses in time
Go out mouldy and bad ovum that is bleaching, prevent water quality from degenerating.
With the method, cultured in vitro survival rate reaches more than 90% in Eriocheir sinensis embryo room.
Claims (5)
1. in-vitro culture method in a kind of Eriocheir sinensis embryo room, including：
(1) it after the Eriocheir sinensis ovigerous crab of fishing being transported to interior, with the tap water dilution bittern after aeration for cultivating, supports
It is 18~19 to grow with the salinity of water, and 18~20 DEG C of water temperature keeps 30~40cm of the depth of water；
(2) Eriocheir sinensis ovigerous crab is selected, fertilized eggs are removed into culture dish and carry out cultured in vitro：In the in vitro of fertilized eggs
In incubation, 9 points of every morning and afternoon 17:00 point is respectively changed a water, and each quantity of exchanged water is 2/3 or so of raw water body, often
Its morning, noon and afternoon stir fertilized eggs 1 time with suction pipe respectively；It keeps sterilizing, be oxygenated simultaneously, choosing bad ovum in time, you can.
2. in-vitro culture method in a kind of Eriocheir sinensis embryo room according to claim 1, it is characterised in that：The step
Suddenly the development of fertilized ova in (2) is to heart beat period.
3. in-vitro culture method in a kind of Eriocheir sinensis embryo room according to claim 1, it is characterised in that：The step
Suddenly the salinity of the culture dish in (2) is 18~19,18~20 DEG C of water temperature.
4. in-vitro culture method in a kind of Eriocheir sinensis embryo room according to claim 1, it is characterised in that：The step
Suddenly the disinfection in (2) is specially that the formaldehyde of a concentration of 50ppm is added in water body.
5. in-vitro culture method in a kind of Eriocheir sinensis embryo room according to claim 1, it is characterised in that：The step
Suddenly the oxygenation in (2) is specially to be oxygenated incessantly for 24 hours in the water body of culture dish.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810105425.7A CN108244009A (en) | 2018-02-02 | 2018-02-02 | In-vitro culture method in a kind of Eriocheir sinensis embryo room |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810105425.7A CN108244009A (en) | 2018-02-02 | 2018-02-02 | In-vitro culture method in a kind of Eriocheir sinensis embryo room |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108244009A true CN108244009A (en) | 2018-07-06 |
Family
ID=62743652
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810105425.7A Pending CN108244009A (en) | 2018-02-02 | 2018-02-02 | In-vitro culture method in a kind of Eriocheir sinensis embryo room |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108244009A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109169431A (en) * | 2018-10-08 | 2019-01-11 | 宁波大学 | A technique for for Scylla paramamosain ovum in-vitro breeding |
| CN109601450A (en) * | 2019-01-29 | 2019-04-12 | 钦州学院 | The method for improving Eriocheir hepuensis breeding survival rate and grazing rate by control salinity |
| CN109619004A (en) * | 2019-01-25 | 2019-04-16 | 中国水产科学研究院东海水产研究所 | A kind of Eriocheir sinensis ovigerous crab indoor culture method |
| CN109644915A (en) * | 2019-01-29 | 2019-04-19 | 钦州学院 | The method for improving Eriocheir hepuensis breeding survival rate by control temperature and taking the photograph bait rate |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1817116A (en) * | 2006-03-16 | 2006-08-16 | 王吉桥 | Chinese crab in-vitro hatching technology |
| CN201451050U (en) * | 2009-06-02 | 2010-05-12 | 上海海洋大学 | Chinese mitten crab circulated water cultivating device capable of controlling dissolved oxygen |
| CN103026989A (en) * | 2011-10-10 | 2013-04-10 | 上海市水产研究所 | Breeding technology for large-size parents of Eriocheir sinensis |
| CN103385184A (en) * | 2013-06-17 | 2013-11-13 | 上海海洋大学 | Method for breeding Eriocheir sinensis in indoor water tank |
| CN104304106A (en) * | 2014-09-26 | 2015-01-28 | 盐城工学院 | High yield culturing method for Chinese mitten juvenile crabs |
| CN105309338A (en) * | 2014-07-22 | 2016-02-10 | 周广安 | Chinese mitten crab breeding method |
| CN106172138A (en) * | 2016-08-02 | 2016-12-07 | 盘锦光合蟹业有限公司 | A kind of crab selection |
| CN106386606A (en) * | 2016-09-13 | 2017-02-15 | 江苏省淡水水产研究所 | A culture method for making Chinese mitten-handed crabs come into market ahead of time |
-
2018
- 2018-02-02 CN CN201810105425.7A patent/CN108244009A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1817116A (en) * | 2006-03-16 | 2006-08-16 | 王吉桥 | Chinese crab in-vitro hatching technology |
| CN201451050U (en) * | 2009-06-02 | 2010-05-12 | 上海海洋大学 | Chinese mitten crab circulated water cultivating device capable of controlling dissolved oxygen |
| CN103026989A (en) * | 2011-10-10 | 2013-04-10 | 上海市水产研究所 | Breeding technology for large-size parents of Eriocheir sinensis |
| CN103385184A (en) * | 2013-06-17 | 2013-11-13 | 上海海洋大学 | Method for breeding Eriocheir sinensis in indoor water tank |
| CN105309338A (en) * | 2014-07-22 | 2016-02-10 | 周广安 | Chinese mitten crab breeding method |
| CN104304106A (en) * | 2014-09-26 | 2015-01-28 | 盐城工学院 | High yield culturing method for Chinese mitten juvenile crabs |
| CN106172138A (en) * | 2016-08-02 | 2016-12-07 | 盘锦光合蟹业有限公司 | A kind of crab selection |
| CN106386606A (en) * | 2016-09-13 | 2017-02-15 | 江苏省淡水水产研究所 | A culture method for making Chinese mitten-handed crabs come into market ahead of time |
Non-Patent Citations (3)
| Title |
|---|
| 王坚毅: "河蟹育苗亲蟹的交配及抱卵蟹的培育", 《特种经济动植物》 * |
| 赵刚 等: "《小水体养殖》", 30 April 2014, 海洋出版社 * |
| 顾功超 等: "中华绒鳌蟹离体卵的人工孵化及其利用", 《渔业机械仪器》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109169431A (en) * | 2018-10-08 | 2019-01-11 | 宁波大学 | A technique for for Scylla paramamosain ovum in-vitro breeding |
| CN109619004A (en) * | 2019-01-25 | 2019-04-16 | 中国水产科学研究院东海水产研究所 | A kind of Eriocheir sinensis ovigerous crab indoor culture method |
| CN109601450A (en) * | 2019-01-29 | 2019-04-12 | 钦州学院 | The method for improving Eriocheir hepuensis breeding survival rate and grazing rate by control salinity |
| CN109644915A (en) * | 2019-01-29 | 2019-04-19 | 钦州学院 | The method for improving Eriocheir hepuensis breeding survival rate by control temperature and taking the photograph bait rate |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101185426B (en) | Specific pathogen free Chinese shrimp zero water-changing seedling production process | |
| CN108244009A (en) | In-vitro culture method in a kind of Eriocheir sinensis embryo room | |
| CN102754610B (en) | Method for improving hippocampus kelloggi fry cultivating rate | |
| CN100372510C (en) | Artificial culture of shrimp parents | |
| CN103404462B (en) | Method for cultivating fries of coilia ectenes in soil pond | |
| CN100586278C (en) | Stichopus japonicus step-by-step hybrid breeding method | |
| CN102283150A (en) | Method for pond culturing Coilia ectenes | |
| CN101019513A (en) | Cultivation process of large no-kernel high quality pearl | |
| CN107148928A (en) | A kind of breeding method of Patinopecten yessoensis and wind vane scallop hybrids | |
| CN109548716A (en) | A kind of strain line hippocampus is young, juvenile fish stage biological breeding method | |
| CN101940183B (en) | Method for cultivating pearl by inducing triploid hyriopsis cumingii | |
| CN101317551A (en) | Artificial nursery stock growing method for Ooctopus ocellatus | |
| CN100506030C (en) | Artificial breeding method for schizothorax | |
| CN101653103A (en) | Breeding method of ultra-large nuclear-free pearl with good quality | |
| CN103004662B (en) | Method for cultivating loach water flower seedlings by using fresh water benthic diatoms | |
| CN103960182B (en) | A kind ofly apply the method that shrimp ovum cultured in vitro sets up Exopalaemon carinicauda family | |
| CN102077790B (en) | Artificially breeding method for Lutraria maximae | |
| CN107751050B (en) | Batched rhinogobio ventralis fry breeding method | |
| CN105052802A (en) | New ostrea rivularis species breeding method | |
| CN103548726B (en) | Establishment and stock breeding method for sinonovacula constricta families | |
| CN108323462A (en) | A kind of breeding method of resistance to less salt chief blood clam seed | |
| MX2007003273A (en) | Shrimp farming in floating cages. | |
| CN106259062B (en) | A kind of breeding method of 1 age of Yarkant Triplophysa fish | |
| CN106614134B (en) | A kind of snakeheaded fish method for culturing seedlings | |
| CN102318572A (en) | Artificial breeding method for Stichopus japonicus |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180706 |