CN105727344A - Composite hemostatic membrane material and preparation method thereof - Google Patents
Composite hemostatic membrane material and preparation method thereof Download PDFInfo
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- CN105727344A CN105727344A CN201410748532.3A CN201410748532A CN105727344A CN 105727344 A CN105727344 A CN 105727344A CN 201410748532 A CN201410748532 A CN 201410748532A CN 105727344 A CN105727344 A CN 105727344A
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Abstract
The invention relates to a composite hemostatic membrane material. The composite hemostatic membrane material comprises a chitosan membrane layer and a starch/gelatin membrane layer, wherein the mass ratio of the chitosan membrane layer to the starch/gelatin membrane layer is within the range between 1 to 1 and 1 to 20. The invention further discloses a preparation method of the composite haemostatic membrane material. The preparation method comprises the following steps: placing a prepared chitosan solution into a mold for drying formation, thus preparing the chitosan membrane layer; and mixing a starch solution and a gelatin solution at the mass ratio of the starch solution to the gelatin solution being between 1 to 1 and 5 to 1, thus preparing a mixed solution, adding the mixed solution onto the chitosan membrane, and carrying out curing formation, thus obtaining the composite haemostatic membrane material. According to the composite haemostatic membrane provided by the invention, several kinds of hemostasis mechanisms can be started simultaneously, so that the efficient hemostasis purpose is achieved, and meanwhile, the composite haemostatic membrane has good adhesion with a wound surface; in addition, the composite haemostatic membrane material prepared by the preparation method disclosed by the invention has good biocompatibility, and absorbable degradation, and has certain antibacterial activity and healing-promoting effects while realizing the effective hemostasis purpose.
Description
Technical field
The invention belongs to biological medicine Material Field, be specifically related to a kind of compound hemostatic membrane material and preparation method thereof.
Background technology
Hemostasis is one of war wound, emergency care of trauma and surgical operation major issue needing solution badly effectively.If patient's severe loss of blood, it is possible to shock, even entail dangers to life and health can be caused.In recent years, development along with medical science, biotechnology and material science, various novel hemostatic materials constantly come out, along with improving constantly hemostatic material performance requirement, exploitation haemostatic effect more preferably, there is good biocompatibility, the hemostatic material in medical use that has no side effect imperative.
Chitosan is a kind of natural absorbable and degradable material being widely used in bio-medical field, has good antibacterial activity and water absorption, it is possible to blood coagulation, infection and promotion wound healing effect are fine to cause the clotting mechanism starting self to promote；But, the haemostatic effect of chitosan itself is limited, less desirable for bigger its haemostatic effect hemorrhage.
Starch is plant polysaccharide, its good biocompatibility, nontoxic, non-stimulated, not easily cause the anaphylaxis of body, when it contacts with wound surface, powerful water absorption can reduce blood flowing speed, makes erythrocyte and PC increase, and excites blood self-solidifying mechanism；Starch hemostatic material representative at present is Arista styptic powder produced in USA, although its haemostatic effect is good, but still Shortcomings: first, adopt epichlorohydrin cross-linked, there is genotoxic potential；Two is that cohesiveness is not good；Three is expensive, limits its application.
Gelatin is a kind of natural absorbable material being widely used in biomedical sector, and good biocompatibility is nontoxic non-stimulated, and molecular structure has substantial amounts of hydroxyl, amino and carboxyl, is a kind of amphiprotic substance, has extremely strong hydrophilic；It is placed in bleeding part, absorbs blood rapidly, cause plaque rupture, promote blood coagulation, reach the purpose of hemostasis, there is adhesion simultaneously, well can attach with wound surface.
Compound hemostatic film prepared by the present invention has efficient anastalsis, has good adhesion simultaneously, well can attach with wound surface, has no report with the Patent Domestic that starch, chitosan and gelatin prepare hemostatic material for raw material.
Summary of the invention
It is an object of the invention to make up above-mentioned the deficiencies in the prior art, it is provided that a kind of efficient hemostatic material with good biocompatibility, absorbable and degradable, safety non-toxic, this hemostatic material has certain biocidal property and promoting healing effect simultaneously.
The preparation method of the compound hemostatic film of the present invention, comprises the steps:
1) weighing a certain amount of chitosan and be dissolved in the dilute acid soln of 0.1mol/L, uniform stirring is to being completely dissolved, and obtaining concentration is 1.0～2.0%(W/V) chitosan solution, standby；
2) weighing a certain amount of starch and be dissolved in deionized water, uniform stirring is to being completely dissolved, and obtaining concentration is 4.0～8.0%(W/V) starch solution, standby；
3) weighing a certain amount of gelatin and be dissolved in deionized water, 30 DEG C of heated and stirred are to being completely dissolved, and obtaining concentration is 0.5～1.5%(W/V) gelatin solution, standby；
4) starch solution step 2 and step 3 prepared and gelatin solution are blended by weight (1:1)～(5:1), prepare starch/gelatin cross-blend liquid, standby；
5) it is placed in mould by the chitosan solution prepared in step 1) curing molding, obtains chitosan rete；Adding starch/gelatin cross-blend liquid the curing molding of preparation in step 4) on described chitosan rete, wherein chitosan rete and starch/gelatin layer weight ratio are (1:1)～(1:20), namely obtain compound hemostatic membrane material.
Preferably, described diluted acid can be the one in dilute hydrochloric acid, spirit of vinegar.
Preferably, described starch is the one that enzyme becomes in the modified starches such as micropore starch, carboxymethyl starch, etherification starch.
The compound hemostatic membrane material that the present invention is prepared in aforementioned manners, is a kind of efficient hemostatic material with good biocompatibility, absorbable and degradable, safety non-toxic, and this hemostatic material has certain biocidal property and promoting healing effect simultaneously.
Specific embodiment
In order to be more fully understood that technical scheme, it is described in further detail by embodiment in detail below.
Embodiment 1:
Weighing 5g Chitosan powder and be dissolved in the dilute hydrochloric acid solution of 0.1mol/L, uniform stirring is to being completely dissolved, and obtaining concentration is 1.0%(W/V) chitosan solution, standby；Weighing 30g starch and be dissolved in deionized water, uniform stirring is to being completely dissolved, and obtaining concentration is 5.0%(W/V) starch solution, standby；Weighing 5g gelatin and be dissolved in deionized water, 30 DEG C of heated and stirred are to being completely dissolved, and obtaining concentration is 0.8%(W/V) gelatin solution, standby；By blended by weight 4:1 to the starch solution prepared in above-mentioned steps and gelatin solution, prepare starch/gelatin cross-blend liquid, standby；First it is placed in mould by the chitosan solution prepared in above-mentioned steps curing molding, obtains chitosan rete；Adding the starch/gelatin cross-blend liquid curing molding that prepare on described chitosan rete, wherein chitosan layer and starch/gelatin layer weight ratio are 1:8, obtain compound hemostatic membrane material.
Embodiment 2:
Weighing 5g Chitosan powder and be dissolved in the dilute acetic acid solution of 0.1mol/L, uniform stirring is to being completely dissolved, and obtaining concentration is 1.0%(W/V) chitosan solution, standby；Weighing 30g starch and be dissolved in deionized water, uniform stirring is to being completely dissolved, and obtaining concentration is 5.0%(W/V) starch solution, standby；Weighing 5g gelatin and be dissolved in deionized water, 30 DEG C of heated and stirred are to being completely dissolved, and obtaining concentration is 0.8%(W/V) gelatin solution, standby；By blended by weight 4:1 to the starch solution prepared in above-mentioned steps and gelatin solution, prepare starch/gelatin cross-blend liquid, standby；First it is placed in mould by the chitosan solution prepared in above-mentioned steps curing molding, obtains chitosan rete；Adding the starch/gelatin cross-blend liquid curing molding that prepare on described chitosan rete, wherein chitosan layer and starch/gelatin layer weight ratio are 1:8, obtain compound hemostatic membrane material.
Embodiment 3:
Weighing 5g Chitosan powder and be dissolved in the dilute hydrochloric acid solution of 0.1mol/L, uniform stirring is to being completely dissolved, and obtaining concentration is 1.5%(W/V) chitosan solution, standby；Weighing 30g starch and be dissolved in deionized water, uniform stirring is to being completely dissolved, and obtaining concentration is 6.0%(W/V) starch solution, standby；Weighing 5g gelatin and be dissolved in deionized water, 30 DEG C of heated and stirred are to being completely dissolved, and obtaining concentration is 1.2%(W/V) gelatin solution, standby；By blended by weight 4:1 to the starch solution prepared in above-mentioned steps and gelatin solution, prepare starch/gelatin cross-blend liquid, standby；First it is placed in mould by the chitosan solution prepared in above-mentioned steps curing molding, obtains chitosan rete；Adding the starch/gelatin cross-blend liquid curing molding that prepare on described chitosan rete, wherein chitosan layer and starch/gelatin layer weight ratio are 1:8, obtain compound hemostatic membrane material.
Embodiment 4:
Weighing 5g Chitosan powder and be dissolved in the dilute hydrochloric acid solution of 0.1mol/L, uniform stirring is to being completely dissolved, and obtaining concentration is 1.0%(W/V) chitosan solution, standby；Weighing 30g starch and be dissolved in deionized water, uniform stirring is to being completely dissolved, and obtaining concentration is 5.0%(W/V) starch solution, standby；Weighing 5g gelatin and be dissolved in deionized water, 30 DEG C of heated and stirred are to being completely dissolved, and obtaining concentration is 0.8%(W/V) gelatin solution, standby；By blended by weight 3:1 to the starch solution prepared in above-mentioned steps and gelatin solution, prepare starch/gelatin cross-blend liquid, standby；First it is placed in mould by the chitosan solution prepared in above-mentioned steps curing molding, obtains chitosan rete；Adding the starch/gelatin cross-blend liquid curing molding that prepare on described chitosan rete, wherein chitosan layer and starch/gelatin layer weight ratio are 1:8, obtain compound hemostatic membrane material.
Embodiment 5:
Weighing 5g Chitosan powder and be dissolved in the dilute hydrochloric acid solution of 0.1mol/L, uniform stirring is to being completely dissolved, and obtaining concentration is 1.0%(W/V) chitosan solution, standby；Weighing 30g starch and be dissolved in deionized water, uniform stirring is to being completely dissolved, and obtaining concentration is 5.0%(W/V) starch solution, standby；Weighing 5g gelatin and be dissolved in deionized water, 30 DEG C of heated and stirred are to being completely dissolved, and obtaining concentration is 0.8%(W/V) gelatin solution, standby；By blended by weight 3:1 to the starch solution prepared in above-mentioned steps and gelatin solution, prepare starch/gelatin cross-blend liquid, standby；First it is placed in mould by the chitosan solution prepared in above-mentioned steps curing molding, obtains chitosan rete；Adding the starch/gelatin cross-blend liquid curing molding that prepare on described chitosan rete, wherein chitosan layer and starch/gelatin layer weight ratio are 1:10, obtain compound hemostatic membrane material.
Embodiment 6:
Weighing 8g Chitosan powder and be dissolved in the dilute hydrochloric acid solution of 0.1mol/L, uniform stirring is to being completely dissolved, and obtaining concentration is 1.5%(W/V) chitosan solution, standby；Weighing 30g starch and be dissolved in deionized water, uniform stirring is to being completely dissolved, and obtaining concentration is 6.0%(W/V) starch solution, standby；Weighing 7g gelatin and be dissolved in deionized water, 30 DEG C of heated and stirred are to being completely dissolved, and obtaining concentration is 1.5%(W/V) gelatin solution, standby；By blended by weight 3:1 to the starch solution prepared in above-mentioned steps and gelatin solution, prepare starch/gelatin cross-blend liquid, standby；First it is placed in mould by the chitosan solution prepared in above-mentioned steps curing molding, obtains chitosan rete；Adding the starch/gelatin cross-blend liquid curing molding that prepare on described chitosan rete, wherein chitosan layer and starch/gelatin layer weight ratio are 1:15, obtain compound hemostatic membrane material.
Embodiment described above; simply presently preferred embodiments of the present invention; not to any formal and substantial restriction of the present invention, the usual variations and alternatives that those skilled in the art carries out within the scope of technical solution of the present invention all should be included in protection scope of the present invention.
Claims (5)
1. a compound hemostatic membrane material, it is characterised in that main component includes chitosan, starch, gelatin；Described membrane material includes chitosan rete and starch/gelatin rete, and its weight ratio is (1:1)～(1:20).
2. compound hemostatic membrane material as claimed in claim 1, it is characterised in that described starch/gelatin rete, the weight ratio of starch and gelatin is (1:1)～(5:1).
3. the preparation method of compound hemostatic membrane material as claimed in claim 1, it is characterised in that be placed in mould by the chitosan solution of preparation drying and moulding, prepares chitosan rete；By starch solution and gelatin solution blended preparation starch/gelatin cross-blend liquid by a certain percentage, blended liquid is added on described chitosan film, curing molding, obtain compound hemostatic film.
4. compound hemostatic membrane material preparation method as claimed in claim 3, it specifically comprises the following steps that
1) weighing a certain amount of chitosan and be dissolved in the dilute acid soln of 0.1mol/L, uniform stirring is to being completely dissolved, and obtaining concentration is 1.0～2.0%(W/V) chitosan solution, standby；
2) weighing a certain amount of starch and be dissolved in deionized water, uniform stirring is to being completely dissolved, and obtaining concentration is 4.0～8.0%(W/V) starch solution, standby；
3) weighing a certain amount of gelatin and be dissolved in deionized water, 30 DEG C of heated and stirred are to being completely dissolved, and obtaining concentration is 0.5～1.5%(W/V) gelatin solution, standby；
4) starch solution step 2 and step 3 prepared and gelatin solution are blended by weight (1:1)～(5:1), prepare starch/gelatin cross-blend liquid, standby；
5) it is placed in mould by the chitosan solution prepared in step 1) curing molding, obtains chitosan rete；Adding starch/gelatin cross-blend liquid the curing molding of preparation in step 4) on described chitosan rete, wherein chitosan rete and starch/gelatin layer weight ratio are (1:1)～(1:20), obtain compound hemostatic membrane material.
5. compound hemostatic membrane material preparation method as claimed in claim 4, it is characterised in that described dilute acid soln can be the one in dilute hydrochloric acid, spirit of vinegar.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106421881A (en) * | 2016-11-17 | 2017-02-22 | 杭州浙海企业管理有限公司 | Chitosan hemostatic wound-healing material and preparation method thereof |
| CN109674768A (en) * | 2018-11-26 | 2019-04-26 | 广州汇朗无纺制品有限公司 | A kind of composite membrane for treating the concurrent II phase exanthemv of skin-type vasculitis |
-
2014
- 2014-12-10 CN CN201410748532.3A patent/CN105727344A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106421881A (en) * | 2016-11-17 | 2017-02-22 | 杭州浙海企业管理有限公司 | Chitosan hemostatic wound-healing material and preparation method thereof |
| CN109674768A (en) * | 2018-11-26 | 2019-04-26 | 广州汇朗无纺制品有限公司 | A kind of composite membrane for treating the concurrent II phase exanthemv of skin-type vasculitis |
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