CN102416194A - Novel chitosan hemostatic sponge and preparation method thereof - Google Patents
Novel chitosan hemostatic sponge and preparation method thereof Download PDFInfo
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- CN102416194A CN102416194A CN2011103824758A CN201110382475A CN102416194A CN 102416194 A CN102416194 A CN 102416194A CN 2011103824758 A CN2011103824758 A CN 2011103824758A CN 201110382475 A CN201110382475 A CN 201110382475A CN 102416194 A CN102416194 A CN 102416194A
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- chitosan
- poloxamer
- sthptic sponge
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Abstract
The invention discloses a novel chitosan hemostatic sponge and a preparation method thereof. The preparation method comprises the following steps of: stirring 5-20 percent of water-soluble chitosan carboxylate or carboxymethyl chitosan and 0.1-5 percent of 407 or 188 poloxamer in water at a low speed to fully dissolving state; adding 0-10 percent of glycerol, stirring at a high speed, foaming for 30 minutes to one hour, and performing injection molding; rapidly refrigerating at the temperature 40 DEG C below zero; demolding; refrigerating and drying till the water content is below 3 percent; and cutting, sterilizing and packing to obtain the novel chitosan hemostatic sponge.
Description
Technical field
The present invention relates to medical technical field, specifically relate to a kind of novel chitosan sthptic sponge and preparation method thereof.
Background technology
Chitosan (chitosan) is to be obtained through deacetylation by the chitin (chitin) that nature extensively exists; Chemical name is polydextrose amine (1-4)-2-amino-B-D glucose; From 1859; After Frenchman Rouget at first obtains chitosan; Premium properties such as the biological functionality of this natural polymer and the compatibility, blood compatibility, safety, microbic resolvability have been obtained major progress by all trades and professions extensive concern at the applied research of numerous areas such as medicine, food, chemical industry, cosmetics, water treatment, METAL EXTRACTION and recovery, biochemistry and biomedical engineering.In practical application, chitosan generally is used for hemostasis through processing sponge behind the crosslinked foaming, discloses like patent CN1071587A and has a kind ofly dissolved chitosan with acetic acid, mixes behind the collagen production technology that becomes sponge with formaldehyde crosslinking; Patent CN131021A discloses a kind of with acid dissolving chitosan, the production technology that glutaraldehyde cross-linking becomes sponge postlyophilization final vacuum drying to form; Patent CN200510024503.3 is cross-linked into the production technology that the sponge postlyophilization forms with the environment-friendly type ion-exchanger after disclosing employing carboxymethyl chitosan glycerol adding.The product that these technology is produced has all used cross-linking agent, and it is residual to have certain toxicity to human body, can not be applied to the hemostasis purposes and be not foamed into sponge.
Summary of the invention
The objective of the invention is to overcome the deficiency of above-mentioned technology, a kind of novel chitosan sthptic sponge and preparation method thereof is provided.
The present invention is employing water-soluble chitosan, the glycerol of realizing through following technical scheme, adds pharmaceutical injection level adjuvant poloxamer as foaming agent, injection molding after the high-speed stirred, and quick-freezing, the demoulding, lyophilization form the hemostatic material of sponge structure.And needn't use cross-linking agent.
Wherein water-soluble chitosan, poloxamer, three kinds of component ratios of glycerol are 5%～20%: 0.1%～5%: 0%～10%.Preferred proportion is 14%: 3.5%: 10%.
Preferred carboxylation of water-soluble chitosan or carboxymethyl chitosan.
Poloxamer model preferred 407 or 188.
Weighing is in container in proportion with chitosan, poloxamer then, and stirring at low speed is extremely dissolved fully, injection molding after high-speed stirred foamed 30 minutes to 1 hour behind the adding ormal weight glycerol, and quick-freezing, the demoulding, lyophilization to moisture is lower than 3%.Cut, sterilization, packing promptly gets.
Wherein optimal process sthptic sponge raw material forms through foaming and twice cryopreparation.Twice freezing minimum temperature all should be below-10 degree.
The invention has the advantages that:
1. the soft high resilience of the sthptic sponge of this prepared; Have high water absorbing force and high-expansion; And visible component such as electronegative erythrocyte, leukocyte, platelet combines in the positively charged molecule of this sthptic sponge and the blood, can produce Blood clotting, need not excite the blood coagulation substance such as prostaglandin, platelet of self; Still can bring into play its haemostatic effect during therefore low, lazy weight, so can shorten bleeding stopping period in anemia or platelet function.In addition, it has good promotion wound healing effect, and has antibacterial and the effect of promotion wound healing, can be applied to medical fields such as surgical hemostasis, tract hemostasis and wound healing.
2. this technology is foamed into and does not adopt cross-linking agent in the sponge process, and adopts medical injectable grade poloxamer, and human body is had avirulence, has good biocompatibility, and sthptic sponge degrades in human body, need not take out by second operation.
The specific embodiment
The specific embodiment below in conjunction with specific embodiment is done further to specify to foregoing of the present invention:
Embodiment 1
With carboxymethyl chitosan 10g, 407 poloxamer 1g weighings in container, add water 100ml stirring at low speed to dissolving fully after, add behind the 2g glycerol high-speed stirred foaming injection molding after 30 minutes to 1 hour ,-40 ℃ of quick-freezings, the demoulding, lyophilization to moisture is lower than 3%.Cut, sterilization, packing promptly gets.
Embodiment 2
With chitosan 14g, 407 poloxamer 3.5g weighings in container, add water 100ml stirring at low speed to dissolving fully after, add behind the 10g glycerol high-speed stirred foaming injection molding after 30 minutes to 1 hour ,-40 ℃ of quick-freezings, the demoulding, lyophilization to moisture is lower than 3%.Cut, sterilization, packing promptly gets.
Embodiment 3
With chitosan 5g, 407 poloxamer 5g weighings in container, add water 100ml stirring at low speed to dissolving fully after, add behind the 10g glycerol high-speed stirred foaming injection molding after 30 minutes to 1 hour ,-40 ℃ of quick-freezings, the demoulding, lyophilization to moisture is lower than 3%.Cut, sterilization, packing promptly gets.
Embodiment 4
With chitosan 20g, 188 poloxamer 0.1g weighings in container, add water 100ml stirring at low speed to dissolving fully after, high-speed stirred foaming is injection molding after 30 minutes to 1 hour ,-40 ℃ of quick-freezings, the demoulding, lyophilization to moisture is lower than 3%.Cut, sterilization, packing promptly gets.
Embodiment 5
With carboxylation or carboxymethyl chitosan 20g, 188 or 407 poloxamer 0.1g weighings in container; After adding water 100ml stirring at low speed to dissolving fully, add 10g glycerol high-speed stirred foaming injection molding after 30 minutes to 1 hour ,-30 ℃ of quick-freezings; The demoulding, lyophilization to moisture is lower than 3%.Cut, sterilization, packing promptly gets.
Embodiment 6
With carboxylation or carboxymethyl chitosan 17g, 188 poloxamer 1g weighings in container, add water 100ml stirring at low speed to dissolving fully after, add 1g glycerol high-speed stirred foaming injection molding after 30 minutes to 1 hour ,-40 ℃ of quick-freezings, the demoulding, lyophilization to moisture is lower than 3%.Cut, sterilization, packing promptly gets.
Embodiment 7
With carboxylation or carboxymethyl chitosan 13g, 188 poloxamer 5g weighings in container, add water 100ml stirring at low speed to dissolving fully after, add 8g glycerol high-speed stirred foaming injection molding after 30 minutes to 1 hour ,-20 ℃ of quick-freezings, the demoulding, lyophilization to moisture is lower than 3%.Cut, sterilization, packing promptly gets.
Embodiment 8
With chitosan 10g, poloxamer 0.1g weighing in container, add water 100ml stirring at low speed to dissolving fully after, add 1g glycerol high-speed stirred foaming injection molding after 30 minutes to 1 hour ,-30 ℃ of quick-freezings, the demoulding, lyophilization to moisture is lower than 3%.Cut, sterilization, packing promptly gets.
Embodiment 9
With chitosan 20g, poloxamer 0.1g weighing in container, add water 100ml stirring at low speed to dissolving fully after, add 10g glycerol high-speed stirred foaming injection molding after 30 minutes to 1 hour ,-40 ℃ of quick-freezings, the demoulding, lyophilization to moisture is lower than 3%.Cut, sterilization, packing promptly gets.
Embodiment 10
With carboxylation or carboxymethyl chitosan 14g, 188 or 407 poloxamer 3.5g weighings in container; After adding water 100ml stirring at low speed to dissolving fully, add 10g glycerol high-speed stirred foaming injection molding after 30 minutes to 1 hour ,-10 ℃ of quick-freezings; The demoulding, lyophilization to moisture is lower than 1%.Cut, sterilization, packing promptly gets.
Claims (8)
1. novel chitosan sthptic sponge, it is characterized in that: said sthptic sponge is become to be grouped into more than three kinds or three kinds by water-soluble chitosan, poloxamer, glycerol etc.
2. chitosan sthptic sponge according to claim 1, it is characterized in that water-soluble chitosan, poloxamer, glycerol etc. more than three kinds or three kinds composition in aqueous solution, combine through series of steps reaction.
3. chitosan sthptic sponge according to claim 1 is characterized in that water-soluble chitosan, poloxamer, three kinds of component ratios of glycerol are 5%～20%: 0.1%～5%: 0%～10%.
4. chitosan sthptic sponge according to claim 1 is characterized in that water-soluble chitosan, poloxamer, three kinds of component ratios of glycerol are 14%: 3.5%: 10%.
5. chitosan sthptic sponge according to claim 1 is characterized in that water-soluble chitosan is carboxylation or carboxymethyl chitosan.
6. chitosan sthptic sponge according to claim 1 is characterized in that the poloxamer model is 407 or 188.
7. the method for preparing of a novel chitosan sthptic sponge, it is characterized in that: said method for preparing step is following:
Weighing is in container in proportion with chitosan, poloxamer, and stirring at low speed is extremely dissolved fully, injection molding after high-speed stirred foamed 30 minutes to 1 hour behind the adding ormal weight glycerol, and quick-freezing, the demoulding, lyophilization to moisture is lower than 3%.Cut, sterilization, packing promptly gets.
8. the method for preparing of chitosan sthptic sponge according to claim 7 is characterized in that: the sthptic sponge raw material must form through foaming and twice cryopreparation in the said method for preparing step.Twice freezing minimum temperature all should be below-10 ℃.
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| CN2011103824758A CN102416194A (en) | 2011-11-11 | 2011-11-11 | Novel chitosan hemostatic sponge and preparation method thereof |
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| CN2011103824758A CN102416194A (en) | 2011-11-11 | 2011-11-11 | Novel chitosan hemostatic sponge and preparation method thereof |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103341202A (en) * | 2013-06-17 | 2013-10-09 | 江苏迪沃生物制品有限公司 | Chitosan sponge surgical dressing and preparation method thereof |
| CN103394115A (en) * | 2013-07-31 | 2013-11-20 | 江苏迪沃生物制品有限公司 | Starch-derived absorbable medical sponge and preparation method thereof |
| CN104353106A (en) * | 2014-11-05 | 2015-02-18 | 张自强 | Composition of fast hemostatic sponge gel and preparation method of composition |
| CN105233324A (en) * | 2015-10-23 | 2016-01-13 | 徐爱军 | Anti-inflammatory antiphlogistic hemostatic sponge and preparing method of anti-inflammatory antiphlogistic hemostatic sponge |
| CN105343924A (en) * | 2015-11-30 | 2016-02-24 | 北京化工大学 | Method of using dopamine for rapidly crosslinking chitosan to prepare hemostatic sponge |
| CN105477679A (en) * | 2015-11-30 | 2016-04-13 | 北京化工大学 | Polysaccharide crosslinking based quick chitosan hemostatic cotton |
| CN105536029A (en) * | 2015-12-25 | 2016-05-04 | 蓝广芊 | Preparation method for chitosan porous hemostatic sponge |
| CN107349458A (en) * | 2017-07-14 | 2017-11-17 | 北京化工大学 | A kind of preparation method of gelatin/plant polyose compound hemostatic sponge |
| CN107349461A (en) * | 2017-07-21 | 2017-11-17 | 北京化工大学 | A kind of preparation method and applications of antibacterial anti hemorrhagic chitosan |
| CN109260511A (en) * | 2018-09-14 | 2019-01-25 | 广州润虹医药科技股份有限公司 | A kind of Injectable porous calcium phosphate bone cement and preparation method thereof |
| CN111714684A (en) * | 2019-03-19 | 2020-09-29 | 广东博与再生医学有限公司 | Bone hemostatic material and preparation method thereof |
| CN111714683A (en) * | 2019-03-19 | 2020-09-29 | 广东博与再生医学有限公司 | Bone hemostatic material and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1670061A (en) * | 2005-03-21 | 2005-09-21 | 上海美宝生命科技有限公司 | Carboxymerhyl chitosan sponge with water-absorbent dilatability and its preparation method and use thereof |
| CN1765423A (en) * | 2005-11-07 | 2006-05-03 | 四川大学 | Method for preparing biology active porous stent material |
-
2011
- 2011-11-11 CN CN2011103824758A patent/CN102416194A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1670061A (en) * | 2005-03-21 | 2005-09-21 | 上海美宝生命科技有限公司 | Carboxymerhyl chitosan sponge with water-absorbent dilatability and its preparation method and use thereof |
| CN1765423A (en) * | 2005-11-07 | 2006-05-03 | 四川大学 | Method for preparing biology active porous stent material |
Cited By (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103341202B (en) * | 2013-06-17 | 2014-05-07 | 江苏迪沃生物制品有限公司 | Chitosan sponge surgical dressing and preparation method thereof |
| CN103341202A (en) * | 2013-06-17 | 2013-10-09 | 江苏迪沃生物制品有限公司 | Chitosan sponge surgical dressing and preparation method thereof |
| CN103394115A (en) * | 2013-07-31 | 2013-11-20 | 江苏迪沃生物制品有限公司 | Starch-derived absorbable medical sponge and preparation method thereof |
| CN104353106A (en) * | 2014-11-05 | 2015-02-18 | 张自强 | Composition of fast hemostatic sponge gel and preparation method of composition |
| CN105233324A (en) * | 2015-10-23 | 2016-01-13 | 徐爱军 | Anti-inflammatory antiphlogistic hemostatic sponge and preparing method of anti-inflammatory antiphlogistic hemostatic sponge |
| CN105343924A (en) * | 2015-11-30 | 2016-02-24 | 北京化工大学 | Method of using dopamine for rapidly crosslinking chitosan to prepare hemostatic sponge |
| CN105477679A (en) * | 2015-11-30 | 2016-04-13 | 北京化工大学 | Polysaccharide crosslinking based quick chitosan hemostatic cotton |
| CN105477679B (en) * | 2015-11-30 | 2018-07-27 | 北京化工大学 | Based on the crosslinked chitosan quick-acting haemostatic powder cotton of polysaccharide |
| CN105536029B (en) * | 2015-12-25 | 2018-07-13 | 蓝广芊 | A kind of preparation method of chitosan multi-porous styptic sponge |
| CN105536029A (en) * | 2015-12-25 | 2016-05-04 | 蓝广芊 | Preparation method for chitosan porous hemostatic sponge |
| CN107349458A (en) * | 2017-07-14 | 2017-11-17 | 北京化工大学 | A kind of preparation method of gelatin/plant polyose compound hemostatic sponge |
| CN107349458B (en) * | 2017-07-14 | 2019-09-13 | 北京化工大学 | A kind of preparation method of gelatin/plant polyose compound hemostatic sponge |
| CN107349461A (en) * | 2017-07-21 | 2017-11-17 | 北京化工大学 | A kind of preparation method and applications of antibacterial anti hemorrhagic chitosan |
| CN107349461B (en) * | 2017-07-21 | 2019-10-29 | 北京化工大学 | A kind of preparation method and applications of antibacterial anti hemorrhagic chitosan |
| CN109260511A (en) * | 2018-09-14 | 2019-01-25 | 广州润虹医药科技股份有限公司 | A kind of Injectable porous calcium phosphate bone cement and preparation method thereof |
| CN111714684A (en) * | 2019-03-19 | 2020-09-29 | 广东博与再生医学有限公司 | Bone hemostatic material and preparation method thereof |
| CN111714683A (en) * | 2019-03-19 | 2020-09-29 | 广东博与再生医学有限公司 | Bone hemostatic material and preparation method thereof |
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Application publication date: 20120418 |