CN105726378A - Cosmetic composition having gene-repairing function - Google Patents
Cosmetic composition having gene-repairing function Download PDFInfo
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- CN105726378A CN105726378A CN201410754546.6A CN201410754546A CN105726378A CN 105726378 A CN105726378 A CN 105726378A CN 201410754546 A CN201410754546 A CN 201410754546A CN 105726378 A CN105726378 A CN 105726378A
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Abstract
The invention relates to a cosmetic composition having a gene-repairing function. The composition is manufactured by extracting natural active components from willow barks and twigs through a hydrothermal method, and heating the obtained natural active components with bifidobacteria cytolysate while performing stirring at 40-50 DEG C. The manufactured composition can effectively prompt gene expression of gene KRT6B and gene KRT10 related to keratin, thereby repairing skin damages, due to various kinds of reasons such as free radical damage resulting from sunlight, in a gene level, and relieving skin aging.
Description
Technical field
The invention belongs to cosmetic field, be specifically related to a kind of cosmetic composition with gene maintenance function, can be used for the making of the cosmetics such as skin base solution.
Background technology
The environmental factors such as sun exposure, air pollution, and self secretion of pressure, emotion induction is uneven etc., and skin all can be caused damage by factor.Especially the skin of face being mainly made up of keratinocyte, is more vulnerable to the impact of these factors, causes skin dark stain formation, wrinkle appearance, old and feeble aggravation.Research shows, it is impaired or lose the reason of expression activity that the appearance of these consequences is largely because skin DNA molecular, makes keratin and the cell related substances cannot normal expression.Wherein KRT6B gene and KRT10 gene are closely related with the expression of keratin and cell fiber reinforcement albumen.Increasing or increasing environmental pollution with the age, its expression activity is gradually lowered, and causes skin aging and damage.Professional field is it is known that the cell lysis thing of yeast, Bifidus etc. can promote the isogenic expression of KRT6B, thus repairing impaired skin from gene aspect.But owing to going deep into that skin is limited, active component and the factor such as skin adhesion is not strong, repairing effect is limited.Simultaneously it is reported that, the combined lifting repairing effect of salicyloyl phytosphingosine and sphingosine derivatives and Bifidus ferment liquid.The synergism of the two can promote related gene expression further on the basis of single thalline cell lysis thing, makes the synthetic quantity of the materials such as keratin strengthen, reaches to repair the purpose of impaired skin.But sphingosine derivatives is still not very good to KRT10 isogenic expressing promoting process degree with this combination of thalline cell lysis thing.
Correlational study shows, willow extract contains multiple active skull cap components, it is possible to effectively permeates skin, dissipating blood stasis for subsidence of swelling, improves skin immunity.Its Patent CN102038081B reports the extracting method of a kind of willow extract, and using extract as feed additive, can effectively prevent Animal diseases.The water extract decapacitation of bark of willow and branch helps, outside active substance infiltration skin, also to provide the natural materials such as phosphoric acid glycosides, base, RNA for gene repair.
Summary of the invention
It is an object of the invention to provide the cosmetic composition with gene maintenance function of a kind of natural improvement, the activation recovering that can sufficiently promote KRT6B, KRT10 gene promotes it to accelerate to express, thus accelerating skin keratin and the synthesis of fiber reinforcement albumen, repair impaired skin.
The present invention realizes especially by following steps:
(1) willow branch and bark are cut into segment, or grind after shearing, can pressurize for reaching temperature with water-ethanol (ethanol content 20%-50%) mixed solvent at 70 ° of C-100 ° of C() skim the cream off milk under condition material, and concentrate, obtain willow extract concentrated solution.
(2) willow extract concentrated solution and the Bifidus C-50 ° of C of cell lysis thing Hybrid Heating to 40 ° hydro-thermal method extracted, is sufficiently stirred for 10-30 minute and makes complex, makes in complex the mass fraction of willow extract at 1%-20%.
(3) complex is cooled to room temperature, and cold preservation is standby at 0 ° of C-4 ° of C.
(4) by combinations such as above-mentioned complex and cosmetic base, wetting agent, spice, preservative, the cosmetics such as skin base solution containing this active compound are made.
In the present invention, willow extract concentrated solution used is willow essence substance, containing various active compositions such as polysaccharide, salicylic acid, phosphoric acid glycosides, base, RNA and lysates, Bifidus cell lysis object depth can be helped to enter Skin Cell, promote the effect probability to KRT6B and KRT10 gene；Simultaneously the bio-molecules such as contained DNA, RNA, phosphoric acid glycosides can be expressed for the desirable genes of Skin Cell and be provided raw material and energy supply material, thus from gene aspect lifting angle albumen, fibrinous compound prbability.
Accompanying drawing explanation
Accompanying drawing show the culture fluid containing heretofore described compositions and matched group culture fluid processes the expression of KRT6B gene and KRT10 gene before and after culture method income earner's skin histology respectively.Skin histology is placed in containing, in the culture fluid of the compositions in the present invention, cultivating 24 hours when 37 ° of C.It is respectively adopted in RNA reverse transcription and pcr amplification technical measurement tissue the expression of KRT6B gene and KRT10 gene before cultivating and after cultivating, the expression increase amount after being processed.Wherein block diagram 1-4 represents the aqueous solution (1) containing 3% ethanol respectively；The mixed aqueous solution (2) of Bifidus cell lysis thing (mass fraction 10%) and ethanol (3%)；Aqueous solution (3) containing 0.1% willow extract concentrated solution；After aqueous solution (4) containing the compositions (in compositions willow extract concentrated solution mass fraction 1%) in the present invention that mass fraction is 10% processes, the expression of KTR6B gene, with the expression of matched group time untreated for 100%.After block diagram 5-8 processes when being illustrated respectively in 1-4, the expression of KTR10 gene.Figure is marked numeral for corresponding gene above each cylindricality relative to the percentage ratio of expression before processing.
Detailed description of the invention
Embodiment 1. prepare active compound as follows: (1) weighs 0.5kg willow bark and 0.5kg willow branch, clean and cut into segment and be placed in reactor.Add the mixed solution of 5kg water-ethanol (ethanol content is 30%) in the reactor.Airtight steaming and decocting 30 minutes when 70 ° of C.Gained solution strainer filtering post-heating concentrates 20 minutes, obtains willow extract concentrated solution.(2) taking the Bifidus cell lysis thing mixing of above-mentioned concentrated solution and 99 times of quality, heating, to 40 ° of C, is sufficiently stirred for 10 minutes and makes complex.(3) complex is cooled to room temperature cold preservation in 4 ° of C when, obtains active compound.By obtained compositions-treated by cultivating the application on human skin tissue obtained.Concrete grammar is be incubated at by skin histology in the culture fluid that composition levels is 10% when 37 ° of C 24 hours, does control experiment simultaneously.As shown in drawings, it can be seen that under the effect of the present composition, KRT6B gene and KRT10 gene expression amount substantially increase.And related gene expression facilitation is all substantially not so good as the compositions in the present invention by independent Bifidus cell lysis thing and willow extract, illustrate that the compound of willow extract and Bifidus cell lysis thing and synergism serve pivotal role to promoting gene expression amount.The skin keratin of part silence and the expression of fiber reinforcement are restarted.In molecular biology level, skin is carried out maintenance.
Embodiment 2. the mode described in embodiment 1 prepares compositions, and is used for the preparation of skin base solution by following material amounts: the compositions in the present invention 9 grams, hyaluronate sodium 0.5 gram, HANSHENGJIAO 0.15 gram, 1,3-PD 5 grams, EDETATE SODIUM 0.1 gram, buffer agent 1 gram, preservative 0.5 gram, Flos Rosae Rugosae quintessence oil 0.05 gram, ethanol 2 grams, limonene 0.03 gram, emulsifying agent 0.02 gram, ultra-pure water 83.5 grams.With common emulsification method, above-mentioned material is made skin base solution, can effectively repair impaired skin, alleviate skin aging.
Embodiment 3.After summer is swum, the volunteer of skin sunburn carries out specific aim maintenance with containing compositions of the present invention.The same volunteer's skin of shoulder is carried out side comparison, side maintenance.Use the reparation skin base solution containing compositions of the present invention in the side of maintenance, smear common sunscreen frost in comparison side.Finding after one week that both sides skin colors all subtracts light, but use reparation skin base solution side redness and disappear and become apparent from, skin sunburn repairing effect is better.Illustrate that the compositions of the present invention has repair to shining injured tissue.
Embodiment 4.By in embodiment 2 preparation containing the reparation skin base solution of the present composition allot to age bracket respectively 20-30 year, 30-40 year, 40-50 year people (be designated as A, B, C group respectively, often organize 10 people) it is suitable for, after continuance of application one week, A group crowd thinks that this skin base solution skin-friendly is good, absorb quickly, it is obviously improved by rear resisting skin allergy performance, and represents that sunscreen is had facilitation；B group crowd thinks, persistently promotes with rear skin shine, and eye pouch position microgroove and pigment improve, and skin absorption is good, and skin quality is improved substantially；C group crowd thinks, significantly reduces with rear face microgroove, and mottle pigment significantly reduces, and skin shine promotes and sense of touch is obviously improved, elastic substantially recovery.
Above-described embodiment all indicates beneficial effects of the present invention, but beneficial effects of the present invention is including, but not limited to this, therefore the present composition and apparent change is applied to other related fields also by the protection of the present invention.
Claims (5)
1. a cosmetic composition with gene maintenance function, it is characterised in that prepared by following steps:
1) willow branch and bark being cut into segment, or grind after shearing, can pressurize for reaching temperature at 70 ° of C-100 ° of C(with water-ethanol (ethanol content 20%-50%) mixed solvent) skim the cream off milk under condition material, and concentrate, obtain willow extract concentrated solution；
2) willow extract concentrated solution and the Bifidus C-50 ° of C of cell lysis thing Hybrid Heating to 40 ° hydro-thermal method extracted, is sufficiently stirred for 10-30 minute and makes complex, makes in complex the mass fraction of willow extract at 1%-20%；
3) complex is cooled to room temperature, and cold preservation is standby at 0 ° of C-4 ° of C.
2. compositions according to claim 1, it is characterised in that willow extract can also be dry land willow, Canescens, the extract of other willow kinds apparent such as Salix alba.
3. compositions according to claim 1, it is characterised in that may be used for the making of the cosmetics such as skin base solution.
4. the making of cosmetics according to claim 3, it is characterised in that mass fraction shared by compositions is more than 1%.
5. a cosmetic composition with gene maintenance function, it is characterised in that by the synergism of willow extract Yu Bifidus cell lysis thing, it is possible to improve the expression efficiency of KRT6B gene and KRT10 gene.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410754546.6A CN105726378A (en) | 2014-12-11 | 2014-12-11 | Cosmetic composition having gene-repairing function |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410754546.6A CN105726378A (en) | 2014-12-11 | 2014-12-11 | Cosmetic composition having gene-repairing function |
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| CN105726378A true CN105726378A (en) | 2016-07-06 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109700998A (en) * | 2019-02-25 | 2019-05-03 | 曾湘红 | A kind of compound skin injury regeneration renovation agent and preparation method thereof |
Citations (3)
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|---|---|---|---|---|
| US20100203094A1 (en) * | 2009-01-12 | 2010-08-12 | L'oreal | Combination of a microorganism and a phytosphingosine derivative, composition, methods of use |
| US20110206721A1 (en) * | 2010-02-19 | 2011-08-25 | Vijaya Nair | Fermented soy nutritional supplements including mushroom components |
| US20130336906A1 (en) * | 2012-06-18 | 2013-12-19 | The Procter & Gamble Company | Method of improving the appearance of aging skin |
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2014
- 2014-12-11 CN CN201410754546.6A patent/CN105726378A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20100203094A1 (en) * | 2009-01-12 | 2010-08-12 | L'oreal | Combination of a microorganism and a phytosphingosine derivative, composition, methods of use |
| US20110206721A1 (en) * | 2010-02-19 | 2011-08-25 | Vijaya Nair | Fermented soy nutritional supplements including mushroom components |
| US20130336906A1 (en) * | 2012-06-18 | 2013-12-19 | The Procter & Gamble Company | Method of improving the appearance of aging skin |
Non-Patent Citations (1)
| Title |
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| 温辉梁: "《世界新药专利生产技术》", 31 March 2007 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109700998A (en) * | 2019-02-25 | 2019-05-03 | 曾湘红 | A kind of compound skin injury regeneration renovation agent and preparation method thereof |
| CN109700998B (en) * | 2019-02-25 | 2022-02-08 | 曾湘红 | Compound skin injury regeneration repairing agent and preparation method thereof |
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Application publication date: 20160706 |