CN105714765A - Microorganism low-temperature grouting and soil reinforcing method - Google Patents

Microorganism low-temperature grouting and soil reinforcing method Download PDF

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Publication number
CN105714765A
CN105714765A CN201610071147.9A CN201610071147A CN105714765A CN 105714765 A CN105714765 A CN 105714765A CN 201610071147 A CN201610071147 A CN 201610071147A CN 105714765 A CN105714765 A CN 105714765A
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temperature
microorganism
low
slip casting
soil
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CN105714765B (en
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邵光辉
冯建挺
朱彦
朱哲
黄容聘
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Nanjing Forestry University
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Nanjing Forestry University
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    • EFIXED CONSTRUCTIONS
    • E02HYDRAULIC ENGINEERING; FOUNDATIONS; SOIL SHIFTING
    • E02DFOUNDATIONS; EXCAVATIONS; EMBANKMENTS; UNDERGROUND OR UNDERWATER STRUCTURES
    • E02D3/00Improving or preserving soil or rock, e.g. preserving permafrost soil
    • E02D3/12Consolidating by placing solidifying or pore-filling substances in the soil
    • CCHEMISTRY; METALLURGY
    • C09DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09KMATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K17/00Soil-conditioning materials or soil-stabilising materials
    • C09K17/40Soil-conditioning materials or soil-stabilising materials containing mixtures of inorganic and organic compounds
    • CCHEMISTRY; METALLURGY
    • C09DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09KMATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K2103/00Civil engineering use

Abstract

A microorganism low-temperature grouting and soil reinforcing method comprises the steps that a, a grouting shock tube 2 of a low-temperature grouting device is driven into a foundation 3; b, a microorganism with biological enzyme centrifugally separated out and removed is used for preparing low-temperature bacterium liquid at the temperature being 4 DEG C, the low-temperature bacterium liquid is mixed with mixed liquid of calcium chloride and urea at the temperature being 4 DEG C to prepare microorganism consolidating liquid, and the temperature is kept to be 4 DEG C, wherein the concentration OD600 of the bacterium liquid is about 1.2, the concentration of the calcium chloride of the mixed liquid ranges from 0.10 mol/L to 0.50 mol/L, and the concentration of the urea of the mixed liquid ranges from 0.10 mol/L-0.50 mol/L; c, the microorganism consolidating liquid 4 is poured into a to-be-cured soil body through a low-temperature grouting device composed of 1,2,5,6 and 7; d, after the temperature of the soil body obtained after grouting is increased to 15 DEG C and is subject to the constant temperature, standing is conducted for 1 h; and e, the step c and the step d are repeated, and grouting is stopped till the microorganism consolidating liquid cannot be injected into the soil body or the strength of the soil body meets the requirement.

Description

The solid indigenous method of a kind of microorganism low-temperature slip casting
Technical field
The present invention relates to the microorganism low-temperature slip casting kind soil strength uniformity of solid land reform and improve the method that grouting process strengthens soil strength, belonging to the technical field that foundation soil body is reinforced.
Background technology
Existing microorganism slip casting soil retaining technology is by injecting microbial inoculum and nutritive salt in soil, utilize microorganism induction calcium carbonate crystal technology (MICP technology), finally hole quickly precipitates out between soil particle calcium carbonate crystal, entirety is connected into by the cementation of calcium carbonate between soil particle, play the effect solidifying the soil body, current engineering mainly utilizes microorganism room temperature slip casting solidify sand and silt.
The technical scheme of prior art one
Microorganism room temperature slip casting solidifies sand and improves intensity mainly through induction inorganic mineral precipitation cement particle, improve shearing rigidity and the ultimate bearing capacity of sand, first microorganism room temperature slip casting inject a certain amount of bacterium solution in sand, then irrigates cementing solution (carbamide-CaCl2) so that the continuous mineralising of microorganism being adsorbed in sand granule generates calcite gel, solidifies sand grains.
The shortcoming of prior art one
For controlling reaction rate, what microorganism room temperature slip casting solidified sand employing is wheel multiple abscess bacterium solution and calcium chloride mixed liquor, and this grouting method solidifies soil body lack of homogeneity, and the cycle is long, and efficiency is low, and stock utilization is low, easily causes injecting paste material waste, and economic benefit is low.
The technical scheme of prior art two
Appropriate deionized water and microorganism are incorporated in dry ground, uniform mixing, make sample, stand a period of time, make antibacterial fully adsorb, then low speed injects the CaCl of low concentration2Mixed solution (MinLeeLee, WeiSongNg, Yasuo, Tanaka.Stress-deformationandcompressibilityresponseofbio-medistedresidualsoils [J] .EcologicalEngineering, 2013,60 (2013): 142-149).
The shortcoming of prior art two
On this grouting method soil body, the microbial biomass of absorption is few, and calcium carbonate nucleating point is few, and the carbonate content that its mineralising produces is low, and soil strength raising is restricted.
The technical scheme of prior art three
Soil mixes microorganism and fluid medium respectively thereof, with the mixed liquor uniform mixing of calcium chloride Yu carbamide, the solidification soil body.(permitted Chaoyang, Zhang Li. the research [J] that silt engineering properties is affected by bio-modification. industrial building .2009,39 (3): 60-61).
The shortcoming of prior art three
Microorganism is mixed and stirred in soil sample process, and original adhesive bond is damaged, and new adhesive bond generates, and soil strength increase rate is only small, Practical Project poor performance, and engineer applied is worth little.
The technical scheme of prior art four
The sand sample made is immersed in microorganism binding liquid, after binding liquid free permeation to soil body hole, microbial mineralization metabolism causes the change of Pore Solution pH value and urase reaction, the generation calcium carbonate crystal solidification soil body (Zhao Qian etc. microorganism induction precipitation of calcium carbonate (MICP) curing soil experimentation [D] Beijing. China University of Geosciences Ph.D. Dissertation, 2014).
The shortcoming of prior art four
Microbial mineralization metabolism causes the change of Pore Solution pH value and urase reaction, makes the carbonate crystallization of generation precipitate out, and the cycle is long, sluggish, easily realizes at laboratory environment, but practical engineering application is relatively difficult.
Summary of the invention
In sum, current engineering utilize the solid earthwork case of microorganism room temperature all there is certain deficiency, microorganism room temperature slip casting solidifies sand grains, sand intensity is greatly improved, but the slip casting cycle is longer, efficiency is low, stock utilization is low, microorganism room temperature slip casting solidifies silt, grouting port easily blocks, silt intensity is uneven, intensity raising is restricted, principles of the invention is: microorganism consolidating fluid is in cryogenic conditions with the injection soil body initial stage before injecting the soil body, microorganism and urase working active are suppressed, reduce biological urase decomposing urea speed, the quantity making the carbanion of generation is few, reaction rate is greatly reduced and delays the generation of calcium carbonate cement, microorganism consolidating fluid can free permeation to enter the soil body cementing without occurring, without blocking soil body duct, and in microorganism consolidating fluid a period of time after injecting the soil body, soil temperature is influenced by ambient temperature progressively to go up, the activity of microorganism and urase gradually steps up, promote quickly generating of calcium carbonate, the calcium carbonate microparticle that early stage cryogenic conditions produces can promote that microorganism adsorption forms biomembrane on soil particle, become the nucleating point of calcarinate crystallization after latter temperature raises, increase the cementing quantity between soil particle and intensity, promote soil body solidification effect.
It is an object of the invention to provide the solid indigenous method of a kind of microorganism low-temperature slip casting, by changing the temperature of slip casting solution, and wheel multiple abscess bacterium solution and mixed liquor are changed into the single single slip casting of liquid of mixing, shorten the slip casting cycle, improve resource utilization, and efficiently solve the non-uniformity problem of microorganism room temperature slip casting, thus form the solid earthwork case of a new microorganism slip casting.
Technical scheme is as follows:
The solid indigenous method of a kind of microorganism low-temperature slip casting, the method step is as follows:
A. according to Practical Project foundation soil situation, select construct summer, by designing requirement, the grouting tube 2 of low temperature grouting equipment is squeezed into sand foundation 3;
B. microorganism after getting rid of existing enzyme with centrifugation configures the mixed liquor of 4 DEG C of low temperature bacterium solution and 4 DEG C of calcium chloride with carbamide and is mixed and made into microorganism consolidating fluid, and to keep temperature be 4 DEG C, wherein bacterial concentration OD600=1.2, calcium chloride concentration is 0.50mol/L, and urea concentration is 0.50mol/L;
C. load, by cool-bag 1,4 DEG C of psychrophile consolidating fluids 4 that proportioning is good, controlled the temperature of liquid in cool-bag 1 by temperature control system 7 and remain at 4 DEG C, open valve 6, inject grouting tube 2 by grouting pump 5, treat that consolidating fluid penetrates into reinforcing area 8, stop slip casting;
D., after ground temperature recovery to 15 DEG C is constant, 1h is stood;
E. c, d operation it is repeatedly performed, until when soil strength reaches requirement or microorganism consolidating fluid cannot inject, stopping slip casting.
The solid indigenous method of described microorganism low-temperature slip casting, the microorganism configuration low temperature bacterium solution after using centrifugation to get rid of existing enzyme, bacterial concentration OD600=1.2.
The solid indigenous method of described microorganism low-temperature slip casting, during slip casting, microorganism used therefor consolidating fluid temperature is at 4 DEG C.
The solid indigenous method of described microorganism low-temperature slip casting, described calcium chloride, urea mixed solution concentration rationally must be determined according to soil species class, it is however generally that, control calcium chloride concentration is 0.10mol/L~0.50mol/L, and urea concentration is 0.10mol/L~0.50mol/L.
The solid indigenous method of described microorganism low-temperature slip casting, described microorganism consolidating fluid is mixed, with low temperature chlorination calcium, urea solution, the single liquid formed by low temperature bacterium solution, and each component order in no particular order is injected simultaneously into the soil body.
The solid indigenous method of described microorganism low-temperature slip casting, after 4 DEG C of described low temperature slip castings there is rise process in soil temperature, and soil temperature gos up to 15 DEG C.
Beneficial effects of the present invention:
(1) low temperature grouting and reinforcing soil effectively improves the uniformity of soil stabilization, overcomes the susceptible to plugging problem of the slip casting mouth of pipe, improves solid soil effect.
(2) fine grained soil can be realized taking turns slip casting by microorganism low-temperature slip casting more, strengthens soil strength.
(3) carrying out respectively with room temperature state compared with note bacterium, note calcium chloride, urea mixed solution, bacterium solution is changed into mixed liquor slip casting in turn and mixes single liquid single slip casting shortening slip casting time by low temperature slip casting, improves work efficiency.
(4) replacement reducing different injecting paste material ejects, and stock utilization is high, saves handling and uses.
Accompanying drawing explanation
Fig. 1. microorganism low-temperature slip casting solid soil device
Accompanying drawing labelling: 1 cool-bag, 2 grouting tubes, 3 grounds, 44 DEG C of microorganism consolidating fluids, 5 grouting pumps, 6 valves, 7 temperature control systems, 8 stabilization zones.
Detailed description of the invention
Embodiment 1
In this example, the solid indigenous method of a kind of microorganism low-temperature slip casting is used for solidifying sand foundation, and the method comprises the following steps:
A. according to Practical Project foundation soil situation, select construct summer, by designing requirement, the grouting tube 2 of low temperature grouting equipment is squeezed into sand foundation 3;
B. microorganism after getting rid of existing enzyme with centrifugation configures the mixed liquor of 4 DEG C of low temperature bacterium solution and 4 DEG C of calcium chloride with carbamide and is mixed and made into microorganism consolidating fluid, and to keep temperature be 4 DEG C, wherein bacterial concentration OD600=1.2, calcium chloride concentration is 0.50mol/L, and urea concentration is 0.50mol/L;
C. load, by cool-bag 1,4 DEG C of psychrophile consolidating fluids 4 that proportioning is good, controlled the temperature of liquid in cool-bag 1 by temperature control system 7 and remain at 4 DEG C, open valve 6, inject grouting tube 2 by grouting pump 5, treat that consolidating fluid penetrates into reinforcing area 8, stop slip casting;
D., after ground temperature recovery to 15 DEG C is constant, 1h is stood;
E. c, d operation it is repeatedly performed, until when soil strength reaches requirement or microorganism consolidating fluid cannot inject, stopping slip casting.
In this example, the reinforcement mechanism of the solid soil of microorganism low-temperature slip casting is: 1, low temperature suppresses microorganism and decomposes the activity of the urase produced, after slip casting completes, in the process that soil temperature gos up, microorganism and urease activity all gradually step up, and service behaviour strengthens gradually, generate calcarinate crystallization.2, the calcium carbonate microparticle that early stage cryogenic conditions produces promotes that microorganism adsorption is on soil particle, and after the later stage soil body heats up, microorganism becomes the nucleating point of cementing calcium carbonate crystal, improves cement production rate and stock utilization.3, microorganism induction precipitation of calcium carbonate cement particle produces cementation solidifying effect.
Embodiment 2
In this example, the solid indigenous method of a kind of microorganism low-temperature slip casting is used for solidifying silt foundation (in place, the natural void ratio of silt is 0.87), and the method comprises the following steps:
A. according to Practical Project foundation soil situation, select construct summer, by designing requirement, the grouting tube of low temperature grouting equipment is squeezed into silt foundation;
B. 4 DEG C of low temperature bacterium solution (the bacterial concentration OD of configuration of the microorganism after existing enzyme are got rid of with centrifugation600=1.2), it is mixed and made into microorganism consolidating fluid with the mixed liquor (calcium chloride concentration is 0.25mol/L, and urea concentration is 0.25mol/L) of 4 DEG C of calcium chloride Yu carbamide, and to keep temperature be 4 DEG C;
C. load, by cool-bag 1,4 DEG C of psychrophile consolidating fluids 4 that proportioning is good, controlled the temperature of liquid in cool-bag 1 by temperature control system 7 and remain at 4 DEG C, open valve 6, inject grouting tube 2 by grouting pump 5, treat that consolidating fluid penetrates into reinforcing area 8, stop slip casting;
D., after ground temperature recovery to 15 DEG C is constant, 1h is stood;
E. c, d operation it is repeatedly performed, until when soil strength reaches requirement or microorganism consolidating fluid cannot inject, stopping slip casting.
In this example, the reinforcement mechanism of the solid soil of microorganism low-temperature slip casting is: 1, low temperature suppresses microorganism and decomposes the activity of the urase produced, after slip casting completes, in the process that soil temperature gos up, microorganism and urease activity all gradually step up, and service behaviour strengthens gradually, generate calcarinate crystallization.2, the calcium carbonate microparticle that early stage cryogenic conditions produces promotes that microorganism adsorption is on soil particle, and after the later stage soil body heats up, microorganism becomes the nucleating point of cementing calcium carbonate crystal, improves cement production rate and stock utilization.3, microorganism induction precipitation of calcium carbonate cement particle produces cementation solidifying effect.
Embodiment 3
In this example, the solid indigenous method of a kind of microorganism low-temperature slip casting is used for solidifying silt foundation (in place, the natural void ratio of silt is 0.42), and the method comprises the following steps:
A. according to Practical Project foundation soil situation, select construct summer, by designing requirement, the grouting tube of low temperature grouting equipment is squeezed into silt foundation;
B. 4 DEG C of low temperature bacterium solution (the bacterial concentration OD of configuration of the microorganism after existing enzyme are got rid of with centrifugation600=1.2), it is mixed and made into microorganism consolidating fluid with the mixed liquor (calcium chloride concentration is 0.10mol/L, and urea concentration is 0.10mol/L) of 4 DEG C of calcium chloride Yu carbamide, and to keep temperature be 4 DEG C;
C. load, by cool-bag 1,4 DEG C of psychrophile consolidating fluids 4 that proportioning is good, controlled the temperature of liquid in cool-bag 1 by temperature control system 7 and remain at 4 DEG C, open valve 6, inject grouting tube 2 by grouting pump 5, treat that consolidating fluid penetrates into reinforcing area 8, stop slip casting;
D., after ground temperature recovery to 15 DEG C is constant, 1h is stood;
E. c, d operation it is repeatedly performed, until when soil strength reaches requirement or microorganism consolidating fluid cannot inject, stopping slip casting.
In this example, the reinforcement mechanism of the solid soil of microorganism low-temperature slip casting is: 1, low temperature suppresses microorganism and decomposes the activity of the urase produced, after slip casting completes, in the process that soil temperature gos up, microorganism and urease activity all gradually step up, and service behaviour strengthens gradually, generate calcarinate crystallization.2, the calcium carbonate microparticle that early stage cryogenic conditions produces promotes that microorganism adsorption is on soil particle, and after the later stage soil body heats up, microorganism becomes the nucleating point of cementing calcium carbonate crystal, improves cement production rate and stock utilization.3, microorganism induction precipitation of calcium carbonate cement particle produces cementation solidifying effect.

Claims (6)

1. the solid indigenous method of microorganism low-temperature slip casting, it is characterised in that the method step is as follows:
A. according to Practical Project foundation soil situation, select construct summer, by designing requirement, the grouting tube (2) of low temperature grouting equipment is squeezed into sand foundation (3);
B. microorganism after getting rid of existing enzyme with centrifugation configures the mixed liquor of 4 DEG C of low temperature bacterium solution and 4 DEG C of calcium chloride with carbamide and is mixed and made into microorganism consolidating fluid, and to keep temperature be 4 DEG C, wherein bacterial concentration OD600=1.2, calcium chloride concentration is 0.50mol/L, and urea concentration is 0.50mol/L;
C. 4 DEG C of psychrophile consolidating fluids (4) that proportioning is good will be loaded in cool-bag (1), the temperature of liquid controlled in cool-bag (1) by temperature control system (7) remains at 4 DEG C, open valve (6), grouting tube (2) is injected by grouting pump (5), treat that consolidating fluid penetrates into reinforcing area (8), stop slip casting;
D., after ground temperature recovery to 15 DEG C is constant, 1h is stood;
E. c, d operation it is repeatedly performed, until when soil strength reaches requirement or microorganism consolidating fluid cannot inject, stopping slip casting.
2. according to the solid indigenous method of claim 1 microorganism low-temperature slip casting, it is characterised in that the microorganism configuration low temperature bacterium solution after using centrifugation to get rid of existing enzyme, bacterial concentration OD600=1.2.
3. the solid indigenous method of microorganism low-temperature slip casting according to claim 1, it is characterised in that during slip casting, microorganism used therefor consolidating fluid temperature is at 4 DEG C.
4. according to the solid indigenous method of claim 1 microorganism low-temperature slip casting, it is characterized in that described calcium chloride, urea mixed solution concentration rationally must be determined according to soil species class, it is said that in general, controlling calcium chloride concentration is 0.10mol/L~0.50mol/L, urea concentration is 0.10mol/L~0.50mol/L.
5. according to the solid indigenous method of claim 1 microorganism low-temperature slip casting, it is characterised in that described microorganism consolidating fluid is mixed, with low temperature chlorination calcium, urea solution, the single liquid formed by low temperature bacterium solution, and each component order in no particular order is injected simultaneously into the soil body.
6. according to the solid indigenous method of claim 1 microorganism low-temperature slip casting, it is characterised in that after 4 DEG C of described low temperature slip castings, soil temperature exists rise process, and soil temperature gos up to 15 DEG C.
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CN106353480A (en) * 2016-08-10 2017-01-25 天津大学 Method for testing microbe cemented fine-grained soil under negative pressure
CN106884424A (en) * 2017-04-10 2017-06-23 中国水利水电科学研究院 A kind of device and construction method that microorganism solidification is carried out in thin silt
CN107100162A (en) * 2017-05-19 2017-08-29 南京林业大学 A kind of solid indigenous method of microorganism gas-liquid cycle slip casting
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CN108049390A (en) * 2017-11-30 2018-05-18 河海大学 A kind of method for breaking born of the same parents' enzyme solution treatment fluid reinforcing silty sand and its soil that desertifies using bacterium
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CN106884424A (en) * 2017-04-10 2017-06-23 中国水利水电科学研究院 A kind of device and construction method that microorganism solidification is carried out in thin silt
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CN107100162A (en) * 2017-05-19 2017-08-29 南京林业大学 A kind of solid indigenous method of microorganism gas-liquid cycle slip casting
CN107119670A (en) * 2017-05-26 2017-09-01 南京林业大学 A kind of solid indigenous method in microorganism negative pressure surface
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CN108049409A (en) * 2017-11-14 2018-05-18 河海大学 A kind of method of the solid soil of microbes synthase inhibitor slip casing by pressure
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CN108589798B (en) * 2018-04-02 2019-06-14 浙江大学 A kind of large diameter pile foundation wash preventing method and device based on MICP technology
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CN108797562A (en) * 2018-06-15 2018-11-13 南京理工大学 A kind of liquefied foundation microorganism grouting method
CN108856248A (en) * 2018-06-19 2018-11-23 浙江工业大学 Solidify the method for the cementing industrial organic waste containing polycyclic aromatic hydrocarbon using yellow cock Salmonella
CN108914924A (en) * 2018-07-05 2018-11-30 浙江科技学院 There are the grouting strengthening method and device in flowing underground water gravel boulder bed
CN110130318A (en) * 2019-05-24 2019-08-16 南京林业大学 A kind of construction method of microorganism mixing-filling stake
CN110144876A (en) * 2019-05-24 2019-08-20 南京林业大学 A kind of microorganism soil-fixing method controlling cementing rate
CN110438975A (en) * 2019-07-23 2019-11-12 河海大学 A kind of microorganism grouting device and construction method for reinforcing deep soft clay
CN110438975B (en) * 2019-07-23 2021-09-17 河海大学 Microbial grouting device for reinforcing deep soft clay and construction method
CN110344392A (en) * 2019-08-07 2019-10-18 南京林业大学 A kind of microorganism soil-fixing apparatus and method of electrostatic slow setting processing
CN111287770A (en) * 2020-03-05 2020-06-16 中煤能源研究院有限责任公司 Method for preventing coal wall caving by reinforcing coal body through microbial grouting
CN112030942A (en) * 2020-08-31 2020-12-04 南京林业大学 Method for improving liquefaction resistance of foundation by silicate mineral decomposing bacteria
CN113585301A (en) * 2021-09-02 2021-11-02 南京大学 Method for treating rock slope collapse by using microbial film and MICP (micro-emulsion-phase phosphate) technology
CN113585301B (en) * 2021-09-02 2022-05-17 南京大学 Method for treating rock slope collapse by using microbial film and MICP (micro-emulsion-phase phosphate) technology

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