CN105714765B - A kind of solid indigenous method of microorganism low-temperature slip casting - Google Patents

A kind of solid indigenous method of microorganism low-temperature slip casting Download PDF

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Publication number
CN105714765B
CN105714765B CN201610071147.9A CN201610071147A CN105714765B CN 105714765 B CN105714765 B CN 105714765B CN 201610071147 A CN201610071147 A CN 201610071147A CN 105714765 B CN105714765 B CN 105714765B
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microorganism
temperature
slip casting
soil
low
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CN105714765A (en
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邵光辉
冯建挺
朱彦
朱哲
黄容聘
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Nanjing Forestry University
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Nanjing Forestry University
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    • EFIXED CONSTRUCTIONS
    • E02HYDRAULIC ENGINEERING; FOUNDATIONS; SOIL SHIFTING
    • E02DFOUNDATIONS; EXCAVATIONS; EMBANKMENTS; UNDERGROUND OR UNDERWATER STRUCTURES
    • E02D3/00Improving or preserving soil or rock, e.g. preserving permafrost soil
    • E02D3/12Consolidating by placing solidifying or pore-filling substances in the soil
    • CCHEMISTRY; METALLURGY
    • C09DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09KMATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K17/00Soil-conditioning materials or soil-stabilising materials
    • C09K17/40Soil-conditioning materials or soil-stabilising materials containing mixtures of inorganic and organic compounds
    • CCHEMISTRY; METALLURGY
    • C09DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09KMATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K2103/00Civil engineering use

Abstract

A kind of method of microorganism low-temperature slip casting, step includes:A. the grouting tube 2 of low temperature grouting equipment is squeezed into ground 3;B. get rid of the microorganism after existing biology enzyme with centrifugation and configure 4 DEG C of low temperature bacterium solutions(Bacterial concentration OD600=1.2 or so), with 4 DEG C of calcium chloride and the mixed liquor of urea(Calcium chloride concentration is 0.10mol/L ~ 0.50mol/L, and urea concentration is 0.10mol/L ~ 0.50mol/L)Microorganism consolidating fluid is mixed and made into, and keeping temperature is 4 DEG C;C. by microorganism consolidating fluid 4 by injecting the soil body to be solidified by 1,2,5,6, the 7 low temperature grouting equipments constituted;D. go up after the soil temperature after slip casting to 15 DEG C of constant temperature, stand 1h;E. repeat the operation of the step of c, d two, when microorganism consolidating fluid can not inject the soil body or soil strength reaches requirement, stop slip casting.

Description

A kind of solid indigenous method of microorganism low-temperature slip casting
Technical field
It is apt to soil strength uniformity the present invention relates to the solid land reform of microorganism low-temperature slip casting and improves the grouting process enhancing soil body The method of intensity, belongs to the technical field of foundation soil body reinforcing.
Background technology
Existing microorganism slip casting soil retaining technology is by injecting microbial inoculum and nutritive salt into soil, utilizing microorganism Calcium carbonate crystallization technique (MICP technologies) is induced, it is finally quick in hole between soil particle to separate out between calcium carbonate crystal, soil particle Connect integral by the cementation of calcium carbonate, play a part of solidifying main normal using microorganism in the soil body, current engineering Warm slip casting solidification sand and silt.
The technical scheme of prior art one
Microorganism normal temperature slip casting solidification sand mainly improves intensity by inducing inorganic mineral to precipitate cement particle, improves sand The shearing rigidity and ultimate bearing capacity of soil, microorganism normal temperature slip casting are injected a certain amount of bacterium solution into sand, then irrigated first Cementing solution (urea-CaCl2) so that the continuous mineralising generation calcite gel of microorganism of the absorption in sand particle, solidify sand Soil particle.
The shortcoming of prior art one
For control reaction rate, microorganism normal temperature slip casting solidification sand uses wheel streamer bacterium solution and mixed with calcium chloride Liquid, grouting method solidification soil body lack of homogeneity, the cycle is long, and efficiency is low, and stock utilization is low, easily causes injecting paste material waste, Economic benefit is low.
The technical scheme of prior art two
Appropriate deionized water and microorganism are incorporated into dry ground, uniform mixing, sample are made, stand a period of time, Bacterium is set fully to adsorb, then low speed injects the CaCl of low concentration2Mixed solution (Min Lee Lee, Wei Song Ng, Yasuo, Tanaka.Stress-deformation and compressibility response of bio-medisted Residual soils [J] .Ecological Engineering, 2013,60 (2013):142-149).
The shortcoming of prior art two
The microbial biomass adsorbed on the grouting method soil body is few, and calcium carbonate nucleating point is few, and the carbonate that its mineralising is produced Content is low, and soil strength is improved and is restricted.
The technical scheme of prior art three
Mix microorganism and its fluid nutrient medium respectively in soil, the mixed liquor uniform mixing with calcium chloride and urea, Gu Change the soil body.(permitted Chaoyang, research [J] industrial buildings .2009 that a jasmine bio-modification influences on silt engineering properties, 39 (3): 60-61)。
The shortcoming of prior art three
Microorganism is mixed and stirred during soil sample, and original adhesive bond is destroyed, and new adhesive bond generation, soil strength is improved Amplitude very little, Practical Project poor performance, engineering application value is small.
The technical scheme of prior art four
The sand sample being made is immersed in microorganism binding liquid, it is micro- after binding liquid free permeation to soil body hole Biomineralization metabolism causes the change of Pore Solution pH value and urase reaction, produces the calcium carbonate crystal solidification soil body (the microorganisms such as Zhao Qian Induce precipitation of calcium carbonate (MICP) curing soil experimental study [D] Beijing China University of Geosciences Ph.D. Dissertation, 2014).
The shortcoming of prior art four
Microbial mineralization metabolism causes the change of Pore Solution pH value and urase reaction, separates out the carbonate crystallization of generation, week Phase is long, and reaction is slow, is easily realized in laboratory environment, but relatively difficult to practical engineering application.
The content of the invention
In summary, there is certain deficiency, microorganism normal temperature using the solid earthwork case of microorganism normal temperature in current engineering Slip casting solidifies sand grains so that sand intensity is greatly improved, but the slip casting cycle is longer, and efficiency is low, material profit Low with rate, microorganism normal temperature slip casting solidification silt, grouting port is easily blocked, and silt intensity is uneven, and intensity is improved and is restricted, this The principle of invention is:Microorganism consolidating fluid is before the injection soil body and the injection soil body is in cryogenic conditions, microorganism and urase initial stage Working active is suppressed, and is reduced biological urase decomposing urea speed, is made the quantity of the carbanion of generation few, significantly drop Low reaction speed and the generation for delaying calcium carbonate cement, microorganism consolidating fluid can enter the soil body without occurring glue with free permeation Knot, will not also block soil body duct, and in microorganism consolidating fluid after the injection soil body a period of time, soil temperature is by environment temperature Influence is progressively gone up, and the activity of microorganism and urase is gradually stepped up, and promotes quickly generating for calcium carbonate, and early stage cryogenic conditions are produced Calcium carbonate microparticle can promote microorganism adsorption in forming biomembrane on soil particle, as calcarinate after latter temperature rise Cementing quantity and intensity between the nucleating point of crystallization, increase soil particle, lift soil body solidification effect.
It is an object of the invention to provide a kind of solid indigenous method of microorganism low-temperature slip casting, by changing the temperature of slip casting solution, And be changed to wheel streamer bacterium solution and mixed liquor to mix the single slip casting of single liquid, shorten the slip casting cycle, improve resource utilization, Er Qieyou Effect solves the non-uniformity problem of microorganism normal temperature slip casting, so as to form the solid earthwork case of a new microorganism slip casting.
Technical scheme is as follows:
A kind of solid indigenous method of microorganism low-temperature slip casting, this method step is as follows:
A. according to Practical Project foundation soil situation, selection summer is constructed, by design requirement by low temperature grouting equipment Grouting tube 2 squeeze into sand foundation 3;
B. get rid of the microorganism after existing biology enzyme with centrifugation and configure 4 DEG C of low temperature bacterium solutions and 4 DEG C of calcium chloride and urine The mixed liquor of element is mixed and made into microorganism consolidating fluid, and keeping temperature is 4 DEG C, wherein bacterial concentration OD600=1.2, calcium chloride is dense Spend for 0.50mol/L, urea concentration is 0.50mol/L;
C. the 4 DEG C of psychrophile consolidating fluids 4 matched will be loaded in cool-bag 1, controls insulation to hold by temperature control system 7 Fluid temperature in device 1 remains at 4 DEG C, opens valve 6, and grouting tube 2 is injected by grouting pump 5, treats that consolidating fluid penetrates into Reinforcing area 8, stops slip casting;
D. after ground temperature recovery is constant to 15 DEG C, 1h is stood;
E. c, d operation is repeated, until when soil strength reaches that requirement or microorganism consolidating fluid can not inject, stopping note Slurry.
The solid indigenous method of described microorganism low-temperature slip casting, the microorganism after existing biology enzyme is got rid of using centrifuging Configure low temperature bacterium solution, bacterial concentration OD600=1.2.
The solid indigenous method of described microorganism low-temperature slip casting, microorganism used therefor consolidating fluid temperature is at 4 DEG C during slip casting.
The solid indigenous method of described microorganism low-temperature slip casting, described calcium chloride, urea mixed solution concentration must be according to soil Species is rationally determined, it is however generally that, it is 0.10mol/L~0.50mol/L to control calcium chloride concentration, and urea concentration is 0.10mol/ L~0.50mol/L.
The solid indigenous method of described microorganism low-temperature slip casting, described microorganism consolidating fluid is by low temperature bacterium solution and low temperature chlorination The single liquid of calcium, urea solution mixing composition, order injects the soil body to each component simultaneously in no particular order.
The solid indigenous method of described microorganism low-temperature slip casting, after 4 DEG C of described low temperature slip castings there is rise process in soil temperature, Soil temperature gos up to 15 DEG C.
Beneficial effects of the present invention:
(1) low temperature grouting and reinforcing soil effectively improves the uniformity of soil stabilization, overcomes that the slip casting mouth of pipe is susceptible to plugging to ask Topic, improves solid native effect.
(2) microorganism low-temperature slip casting can realize many wheel slip castings to fine grained soil, strengthen soil strength.
(3) note bacterium, note calcium chloride are carried out respectively with normal temperature state, compared with urea mixed solution, low temperature slip casting by bacterium solution with Slip casting is changed to mix single liquid single slip casting shortening slip casting time mixed liquor in turn, improves operating efficiency.
(4) the replacement ejection of different injecting paste materials is reduced, stock utilization is high, saves processing cost.
Brief description of the drawings
The solid native device of Fig. 1 microorganism low-temperatures slip casting
Reference:1-cool-bag, 2-grouting tube, 3-ground, 4-4 DEG C of microorganism consolidating fluids, 5-slip casting Pump, 6-valve, 7-temperature control system, 8-stabilization zone.
Embodiment
Embodiment 1
In this example, a kind of solid indigenous method of microorganism low-temperature slip casting is used to solidify sand foundation, and this method includes following step Suddenly:
A. according to Practical Project foundation soil situation, selection summer is constructed, by design requirement by low temperature grouting equipment Grouting tube 2 squeeze into sand foundation 3;
B. get rid of the microorganism after existing biology enzyme with centrifugation and configure 4 DEG C of low temperature bacterium solutions and 4 DEG C of calcium chloride and urine The mixed liquor of element is mixed and made into microorganism consolidating fluid, and keeping temperature is 4 DEG C, wherein bacterial concentration OD600=1.2, calcium chloride is dense Spend for 0.50mol/L, urea concentration is 0.50mol/L;
C. the 4 DEG C of psychrophile consolidating fluids 4 matched will be loaded in cool-bag 1, controls insulation to hold by temperature control system 7 Fluid temperature in device 1 remains at 4 DEG C, opens valve 6, and grouting tube 2 is injected by grouting pump 5, treats that consolidating fluid penetrates into Reinforcing area 8, stops slip casting;
D. after ground temperature recovery is constant to 15 DEG C, 1h is stood;
E. c, d operation is repeated, until when soil strength reaches that requirement or microorganism consolidating fluid can not inject, stopping note Slurry.
In this example, the reinforcement mechanism of the solid soil of microorganism low-temperature slip casting is:1st, low temperature suppresses microorganism and its decomposed to produce Urase activity, after the completion of slip casting, soil temperature go up during, microorganism and urease activity are gradually stepped up, work Making performance gradually strengthens, generation calcarinate crystallization.2nd, the calcium carbonate microparticle that early stage cryogenic conditions are produced promotes microorganism to inhale Invest on soil particle, microorganism turns into the nucleating point of cementing calcium carbonate crystal after the heating of the later stage soil body, improve cement generation Rate and stock utilization.3rd, microorganism induction precipitation of calcium carbonate cement particle produces cementation solidifying effect.
Embodiment 2
In this example, (silt is natural in place for solidifying silt foundation for a kind of solid indigenous method of microorganism low-temperature slip casting Void ratio is that 0.87), this method comprises the following steps:
A. according to Practical Project foundation soil situation, selection summer is constructed, by design requirement by low temperature grouting equipment Grouting tube squeeze into silt foundation;
B. get rid of the microorganism after existing biology enzyme with centrifugation and configure 4 DEG C of low temperature bacterium solution (bacterial concentration OD600= 1.2), the mixed liquor (calcium chloride concentration is 0.25mol/L, and urea concentration is 0.25mol/L) with 4 DEG C of calcium chloride and urea is mixed Microorganism consolidating fluid is made, and keeping temperature is 4 DEG C;
C. the 4 DEG C of psychrophile consolidating fluids 4 matched will be loaded in cool-bag 1, controls insulation to hold by temperature control system 7 Fluid temperature in device 1 remains at 4 DEG C, opens valve 6, and grouting tube 2 is injected by grouting pump 5, treats that consolidating fluid penetrates into Reinforcing area 8, stops slip casting;
D. after ground temperature recovery is constant to 15 DEG C, 1h is stood;
E. c, d operation is repeated, until when soil strength reaches that requirement or microorganism consolidating fluid can not inject, stopping note Slurry.
In this example, the reinforcement mechanism of the solid soil of microorganism low-temperature slip casting is:1st, low temperature suppresses microorganism and its decomposed to produce Urase activity, after the completion of slip casting, soil temperature go up during, microorganism and urease activity are gradually stepped up, work Making performance gradually strengthens, generation calcarinate crystallization.2nd, the calcium carbonate microparticle that early stage cryogenic conditions are produced promotes microorganism to inhale Invest on soil particle, microorganism turns into the nucleating point of cementing calcium carbonate crystal after the heating of the later stage soil body, improve cement generation Rate and stock utilization.3rd, microorganism induction precipitation of calcium carbonate cement particle produces cementation solidifying effect.
Embodiment 3
In this example, (silt is natural in place for solidifying silt foundation for a kind of solid indigenous method of microorganism low-temperature slip casting Void ratio is that 0.42), this method comprises the following steps:
A. according to Practical Project foundation soil situation, selection summer is constructed, by design requirement by low temperature grouting equipment Grouting tube squeeze into silt foundation;
B. get rid of the microorganism after existing biology enzyme with centrifugation and configure 4 DEG C of low temperature bacterium solution (bacterial concentration OD600= 1.2), the mixed liquor (calcium chloride concentration is 0.10mol/L, and urea concentration is 0.10mol/L) with 4 DEG C of calcium chloride and urea is mixed Microorganism consolidating fluid is made, and keeping temperature is 4 DEG C;
C. the 4 DEG C of psychrophile consolidating fluids 4 matched will be loaded in cool-bag 1, controls insulation to hold by temperature control system 7 Fluid temperature in device 1 remains at 4 DEG C, opens valve 6, and grouting tube 2 is injected by grouting pump 5, treats that consolidating fluid penetrates into Reinforcing area 8, stops slip casting;
D. after ground temperature recovery is constant to 15 DEG C, 1h is stood;
E. c, d operation is repeated, until when soil strength reaches that requirement or microorganism consolidating fluid can not inject, stopping note Slurry.
In this example, the reinforcement mechanism of the solid soil of microorganism low-temperature slip casting is:1st, low temperature suppresses microorganism and its decomposed to produce Urase activity, after the completion of slip casting, soil temperature go up during, microorganism and urease activity are gradually stepped up, work Making performance gradually strengthens, generation calcarinate crystallization.2nd, the calcium carbonate microparticle that early stage cryogenic conditions are produced promotes microorganism to inhale Invest on soil particle, microorganism turns into the nucleating point of cementing calcium carbonate crystal after the heating of the later stage soil body, improve cement generation Rate and stock utilization.3rd, microorganism induction precipitation of calcium carbonate cement particle produces cementation solidifying effect.

Claims (2)

1. the solid indigenous method of a kind of microorganism low-temperature slip casting, it is characterised in that this method step is as follows:
A. according to Practical Project foundation soil situation, selection summer is constructed, by design requirement by the note of low temperature grouting equipment Starch floral tube(2)Squeeze into sand foundation(3);
B. got rid of the microorganism after existing biology enzyme and configured 4 DEG C of low temperature bacterium solutions and 4 DEG C of calcium chloride and urea with centrifuging Mixed liquor is mixed and made into microorganism consolidating fluid, and keeping temperature is 4 DEG C, wherein bacterial concentration OD600=1.2, calcium chloride concentration is 0.10~0.50mol/L, urea concentration is 0.10~0.50mol/L;
C. by cool-bag(1)It is interior to load the 4 DEG C of psychrophile consolidating fluids matched(4), by temperature control system(7)Control insulation Container(1)Interior fluid temperature remains at 4 DEG C, opens valve(6), pass through grouting pump(5)Inject grouting tube(2), treat Consolidating fluid penetrates into reinforcing area(8), stop slip casting;
D. after ground temperature recovery is constant to 15 DEG C, 1h is stood;
E. c, d operation is repeated, until when soil strength reaches that requirement or microorganism consolidating fluid can not inject, stopping slip casting.
2. a kind of solid indigenous method of microorganism low-temperature slip casting according to claim 1, it is characterised in that described microbiological gum Knot liquid mixes the single liquid constituted with low temperature chlorination calcium, urea solution by low temperature bacterium solution, and each component is in no particular order sequentially simultaneously Inject the soil body.
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