CN108049390A - A kind of method for breaking born of the same parents' enzyme solution treatment fluid reinforcing silty sand and its soil that desertifies using bacterium - Google Patents
A kind of method for breaking born of the same parents' enzyme solution treatment fluid reinforcing silty sand and its soil that desertifies using bacterium Download PDFInfo
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- CN108049390A CN108049390A CN201711237474.8A CN201711237474A CN108049390A CN 108049390 A CN108049390 A CN 108049390A CN 201711237474 A CN201711237474 A CN 201711237474A CN 108049390 A CN108049390 A CN 108049390A
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- born
- bacterium
- same parents
- enzyme solution
- treatment fluid
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- E—FIXED CONSTRUCTIONS
- E02—HYDRAULIC ENGINEERING; FOUNDATIONS; SOIL SHIFTING
- E02D—FOUNDATIONS; EXCAVATIONS; EMBANKMENTS; UNDERGROUND OR UNDERWATER STRUCTURES
- E02D3/00—Improving or preserving soil or rock, e.g. preserving permafrost soil
- E02D3/12—Consolidating by placing solidifying or pore-filling substances in the soil
Abstract
The invention discloses a kind of methods for breaking born of the same parents' enzyme solution treatment fluid reinforcing silty sand and its soil that desertifies using bacterium, it is characterized in that, using born of the same parents' enzyme solution is broken including bacterium born of the same parents' enzyme solution treatment fluid is broken with the bacterium of corresponding inorganic consolidating fluid treatment fluid, silty sand and desertification soil sample are carried out repeating to pour into processing 4 10 times, it can be by its strength enhancing to 0.5 2Mpa.When repeating to pour into processing to silty sand sample progress multipass, each treating capacity is 0.3 2L/m3.When interval 6 12 is small between adjacent number of processes.Processing of the present invention for silty sand and the soil that desertifies is broken born of the same parents' enzyme solution using bacterium and is greatly promoted compared to simple bacterial immobilisation processing processing depth, and curing degree is more uniform, and processing procedure is simple and easy to do, and cost is cheaper, easily operated suitable for Practical Project.
Description
Technical field
The invention belongs to geotechnical engineering, microorganism curing technology fields, are related to a kind of utilization bacterium and break born of the same parents' enzyme solution treatment fluid
The method for reinforcing silty sand and its soil that desertifies.
Background technology
Microorganism curing technology is swift and violent in the development of geotechnical engineering field in recent years, has for the improvement of soil mass property positive
Effect, particularly sand, due to its good penetrability, be particularly suitable for handling using microbial process, scholar both domestic and external
A series of research has been carried out in microorganism curing for silty sand and its soil that desertifies.
But in Practical Project, handle, associated with the fine grained problems demand such as silty sand liquefaction solves Soft Soil,
Existing microorganism curing technology also encounters bottleneck in the processing of powder.Since bacteria particles scale is larger, bacterium in addition
The adsorptivity of itself, it is limited to handle depth when causing bacterial immobilisation silty sand, cures result lack of homogeneity；If take bacterium solution pre-
It first admixes the modes such as sand material, compound grouting under pressure and carries out microorganism curing, but be difficult to apply to engineering field or cost is too high
It is difficult to large-scale operation.
Microorganism solidification process can effectively improve the property of the soil body, such as Chinese patent application (application number：
201610014417.2) a kind of microorganism combines the bracing means and its reinforcement means that vacuum drainage reinforces sand foundation, by it
Applied to sand foundation reinforce, but simple microorganism curing when handling silty sand due to particle scale, crystalline
The reasons such as state can cause the inhomogeneities (its treatment effect can die-off with depth increase) for the treatment of effect, will also influence final soil
The treatment effect that body is reinforced.
In short, also there are following defects for art methods of the processing containing powder sand and sandization soil：1st, due to powder
Particle is thinner, and bacterium is difficult into underground layer depths, it is impossible to the effectively deeper soil of processing；2nd, due to inside soil body oxygen content very
Low, bacterial activity is suppressed, and inter-process is with obvious effects to be inferior to surface layer；3rd, treatment effect is very uneven, and there are some processing are thin
Weakness influences final process effect.
The content of the invention
The defects of it is an object of the invention to overcome the prior art, provides a kind of utilization bacterium and breaks the reinforcing of born of the same parents' enzyme solution treatment fluid
The method of silty sand and its soil that desertifies, treatment effect is uniform, the high treating effect of final soil stabilization.Processing side of the invention
Method is simple and easy to do, workable, of low cost, and process cycle is short.
In order to solve the above technical problems, the present invention uses following technical scheme.
A kind of method that born of the same parents' enzyme solution treatment fluid reinforcing silty sand is broken using bacterium of the present invention, which is characterized in that utilize
Born of the same parents' enzyme solution is broken including bacterium and breaks born of the same parents' enzyme solution treatment fluid with the bacterium of corresponding inorganic consolidating fluid treatment fluid, and silty sand sample is carried out
Multipass repeats to pour into processing, can be by silty sand strength enhancing to 0.5-2Mpa.
Further, born of the same parents' enzyme solution treatment fluid is broken using the bacterium, silty sand sample is carried out repeating to pour into processing
4-10 times.
When repeating to pour into processing to silty sand sample progress multipass, each treating capacity is 0.3-2L/m3.Adjacent
When interval 6-12 is small between reason number.
A kind of method that born of the same parents' enzyme solution treatment fluid reinforcing desertification soil is broken using bacterium of the present invention, which is characterized in that utilize bag
It includes bacterium and breaks born of the same parents' enzyme solution and break born of the same parents' enzyme solution treatment fluid with the bacterium of corresponding inorganic consolidating fluid treatment fluid, weight is carried out to sandy land surface
It sprays 1-4 times again.
Further, born of the same parents' enzyme solution treatment fluid is broken using the bacterium sandy land surface is carried out to repeat sprinkling processing
When, the treating capacity sprayed every time is 1-3L/m3。
In addition, when repeat sprinkling processing to sandy land surface, 6-12h is spaced between adjacent number of processes.
Compared with prior art, the present invention includes advantages below and advantageous effect：
1. the present invention breaks born of the same parents' enzyme solution treatment fluid using bacterium, consolidation process can be carried out to silty sand and the soil that desertifies.Due to
Bacterium breaks scale smaller after born of the same parents, and adsorptivity is also opposite compared with indigenous bacteria to be weakened, the treatment fluid curing process silty sand and the soil that desertifies
When the calcium carbonate crystal that is formed it is more uniform, final soil strength also higher, treatment effect is uniform, more has in engineering application
It is of practical significance.
2nd, in the present invention, after bacterium breaks born of the same parents, the initial urea decomposition rate of bacterium solution improves 1.5 times to 2 times, significantly
Time cost is improved, if broken born of the same parents' enzyme solution to be diluted to the hydrolysis of urea activity same with original bacteria liquid phase, gained enzyme solution body
Product increases the material cost that culture bacterium for original 1.2 times to 1.6 times, is then greatly saved；
3. cell crashing ratio is more than 98% in the present invention, bacterial content is very low in enzyme solution, minimum on the influence of original environment,
People can also significantly be cut down for the fear there are a large amount of bacteriums in original processing method.
4. processing method of the present invention is simple and easy to do, workable, of low cost, and process cycle is short.
Description of the drawings
Fig. 1 is the silty sandy soil Ottawa sand broken using bacterium when born of the same parents' enzyme solution treatment fluid reinforces silty sand of the present invention
Grade figure.
Fig. 2 be the present invention using bacterium break born of the same parents' enzyme solution treatment fluid reinforce silty sand when silty sandy soil silica flour grade
Figure.
Fig. 3 is the desertification grogs grade figure that born of the same parents' enzyme solution treatment fluid reinforcing desertification soil is broken using bacterium of the present invention.
Specific embodiment
The present invention is described in further details with reference to embodiment.
A kind of method that born of the same parents' enzyme solution treatment fluid reinforcing silty sand is broken using bacterium of the present invention, which is characterized in that utilize
Born of the same parents' enzyme solution is broken including bacterium and breaks born of the same parents' enzyme solution treatment fluid with the bacterium of corresponding inorganic consolidating fluid treatment fluid, and silty sand sample is carried out
Multipass repeats to pour into processing, can be by silty sand strength enhancing to 0.5-2Mpa.
In actual treatment, born of the same parents' enzyme solution treatment fluid is broken using the bacterium, silty sand sample is repeated and pours into place
Reason 4-10 times.Wherein, handle 4 times it is just effective, 10 times the good results are evident for processing, can form hard entirety.Each place
Reason amount is 0.3-2L/m3.Treating capacity control can obtain preferable effect in the range, and save time and cost.Phase
When interval 6-12 is small between adjacent number of processes.So that urea hydrolysis fully carries out, processing cost can be saved, and can be improved
Product crystal habit strengthens consolidation effect.Material cost can be increased if interval time is too short, interval time is too long to be increased
The time cost and cost of labor of construction, can obtain better effects while cost is reduced in the range of this time.
First, the embodiment of silty sand curing process：
1. the preparation process of silty sand sample：
(1) prepare 4 parts of weight be 200g cylindric silty sand sample 1,2,3,4, diameter 40mm, height 80mm,
Gross mass is 168g, and wherein sand content in Ottawa is 85%, and powder (2500 mesh silica flour) content is 15%, divides three layers and hits reality.
The Ottawa sand of its silty sand sample and the grading situation of silica flour are shown in Fig. 1 and Fig. 2.
(2) in the needle tubing bottom of four internal diameter 40mm, the circular filter paper of upper diameter 40mm is padded respectively, is spread in syringe inner wall
Vaseline and attached last layer projection paper facilitate the extraction of sample after the completion of processing.Then point 5 addition silty in four needle tubings
Reality is hit in sand sample, each 40g, layering, and final height is controlled in 10cm.(floor height is controlled in 2cm).
2. the curing process step of silty sand sample：
(1) four parts of 12ml is taken to break born of the same parents' enzyme solution and 36ml treatment fluids, bacterium is synthesized after mixing and breaks born of the same parents' enzyme solution treatment fluid,
It pours into respectively in above-mentioned needle tubing 1,2,3,4.
(2) above-mentioned bacterium will be poured into sample needle tubing 1,2,3,4 respectively and breaks born of the same parents' enzyme solution treatment fluid, carry out curing process 4,6,
8th, 10 times, 12h is spaced between reason per around.
(3) after last time processing terminates 12h, edge projection paper is clamped, sample is extracted and carries out dependence test.
3. the solidification effect test of silty sand：
After silty sand curing process, unconfined compressive strength test is carried out respectively to sample 1,2,3,4, measures result
It see the table below 1.
1. sample of table, 1,2,3,4 non-confining compression strength test result
Careful collection sample 4 tested without lateral spacing after the completion of soil sample, take the edge of its top, middle part and bottom section respectively
It with center position soil sample, is successively dissolved with ultra-pure water and 6mol/L hydrochloric acid standard solutions, calcium in lysate is measured by EDTA
Ion concentration, then the calcium carbonate content being converted into pattern see the table below 2.
2. sample of table, 4 different position calcium carbonate is distributed
| Position | Calcium carbonate content (%) | Position | Calcium carbonate content (%) |
| Tip section edge | 7.71 | Tip section center | 7.92 |
| Middle part section edges | 6.36 | Middle part kernel of section | 6.68 |
| Bottom section edge | 5.87 | Bottom section center | 5.86 |
A kind of method that born of the same parents' enzyme solution treatment fluid reinforcing desertification soil is broken using bacterium of the present invention, which is characterized in that utilize bag
It includes bacterium and breaks born of the same parents' enzyme solution and break born of the same parents' enzyme solution treatment fluid with the bacterium of corresponding inorganic consolidating fluid treatment fluid, weight is carried out to sandy land surface
It sprays 1-4 times again.Wherein, handle 1 time it is just effective, sandy soil are fixed substantially, and 4 times the good results are evident for processing, can on surface layer
Form hard housing, and durability is good.The treating capacity sprayed every time is 1-3L/m3.Treating capacity control in the range can be with
Preferable effect is obtained, and saves time and cost.6-12h is spaced between adjacent number of processes.So as to urea hydrolysis
It fully carries out, processing cost can be saved, and product crystal habit can be improved, strengthen consolidation effect.If interval time is too
Short to increase material cost, the interval time too long time cost and cost of labor that can increase construction can in the range of this time
To obtain better effects while cost is reduced.
Calcium salt preferentially selects calcium nitrate, and nitrate ion therein contributes to the growth of plant, is covered for the vegetation in later stage
Lid process has gain effect.
2nd, the embodiment for the native curing process that desertifies：
1. the step of native curing process that desertifies：
(1) 4 parts of native (gathering from Gansu Wuwei sandy land) samples that desertify are prepared in cuboid pallet, long 24cm is wide
17cm, high 3.8m, grain composition are shown in Fig. 3.
(2) sample 1,2,3,4 spray respectively bacterium break born of the same parents' enzyme solution treatment fluid (wherein, respectively containing bacterium break born of the same parents' enzyme solution 12ml,
Corresponding inorganic consolidating fluid treatment fluid 36ml), curing process 1,2,3,4 times is spaced 12h, nature after handle per around between reason
It air-dries 7 days.
2. the measure of merit for the native curing process that desertifies
Small-sized wind tunnel experiment is carried out respectively to sample 1,2,3,4 after the native curing process that desertifies, measures that the result is shown in the following table 3.
Table 1. desertifies 1,2,3,4 small-sized wind tunnel experimental result of sample after native curing process
Bacterium is broken the process of born of the same parents' enzyme solution treatment fluid reinforcing silty sand and its soil that desertifies by the present invention, is to utilize hydrolysis of urea
The rising for reacting ph in the carbonate generated and reaction process promotes the crystallization of calcite, plays filling and cementing effect, can
Effectively improve the intensity of silty sand and the soil that desertifies.
The bacterium that bacterium of the present invention is broken in born of the same parents' enzyme solution treatment fluid breaks born of the same parents' enzyme solution and corresponding inorganic consolidating fluid treatment fluid
Volume ratio be 1:3-1:5.
The bacterium breaks sonicator after born of the same parents' crude enzyme liquid adds in buffer protection liquid by aerobic bacterial solution and crushes and obtain
, the inorganic consolidating fluid treatment fluid includes following component：Urea 0.2-2mol/L, calcium salt 0.2-2mol/L, the two concentration ratio
For 2:1-1:2.Wherein calcium salt is soluble calcium salt, can be one or more of calcium nitrate, calcium chloride, calcium acetate.It is supported well
Type bacterium is hydrolysis of urea bacterium, and concentration is 107-109A/L.The aerobic bacterium uses thin with urea capacity of decomposition
Bacterium can be that Pasteur gives birth to spore sarcine (Latin generic name：Sporosarcina pasteurii, bacterial strain number is for example：ATCC
11859) or it is terrabacter tumescens (Latin generic name：Terrabacter tumescens, bacterial strain number is for example：ATCC21109),
Enrichment culture ultimate density is in 107-109/L.
Above-mentioned buffer protection liquid includes following component：Sodium chloride 8-10g/L, ethylenediamine tetra-acetic acid 0.001-0.002mol/
L, potassium dihydrogen phosphate 0.02-0.03mol/L, disodium hydrogen phosphate 0.07-0.08mol/L.
The culture medium prescription that the enrichment culture process uses is：Yeast extract powder 15-25g/L, ammonium chloride 8-12g/
L, Nickel dichloride hexahydrate 20-25mg/L, Manganous sulfate monohydrate 10-15mg/L adjust ph to 8.5-9.
The enrichment culture process is preferably passed through oxygen, speed 1-2L/min, and strain is added in after culture medium as setting
Constant temperature degree is to cultivate 16-24h in 25-35 DEG C of constant-temperature table, you can bacterium solution needed for acquisition.
The bacterium solution centrifugal process uses low-temperature and high-speed centrifuge, sets centrifuging temperature as 2-6 DEG C, rotating speed 4000-
6000r/s, centrifugation time 10-15min.
Broken born of the same parents' process uses ultrasonic disruption, and sonicator frequency selects 20-30KHZ, and power uses
200-300W, power regulation are adjusted to 80% to 90%, and ultrasonic transformer uses 9 diameter of Φ 6 or Φ, and ultrasonic time is worked using 3-8s,
The operating mode that 3-8s rests, and net cycle time is calculated by every milliliter of mixed solution 12s-24s, final bacterium breakdown ratio exists
More than 98%.
The broken liquid centrifugal process uses low-temperature and high-speed centrifuge, sets centrifuging temperature as 2-6 DEG C, rotating speed is
6000-8000r/s, centrifugation time 15-20min.
The preparation process that a kind of bacterium of the present invention breaks born of the same parents' enzyme solution treatment fluid comprises the following steps：
(1) microbiological culture media 500ml is prepared, includes yeast extract powder 20g/L, ammonium chloride 10g/L, Nickel dichloride hexahydrate
24mg/L, Manganous sulfate monohydrate 10mg/L adjust ph to 9, using pasteurization in 121 DEG C of autoclave at high temperature 30min；
(2) Pasteur is given birth to spore sarcine strain to add in culture medium, is passed through oxygen, speed 1.5L/min, by strain
Add in after culture medium as design temperature be cultivated in 30 DEG C of constant-temperature table obtain for 24 hours needed for bacterium solution；
(3) bacterium solution centrifuges 10min under 4 DEG C, 4000-6000r/s rotating speeds, pours out supernatant, and the wet bacterial strain of gained adds in slow
Fliud flushing, buffer solution include sodium chloride 9g/L, ethylenediamine tetra-acetic acid 0.002mol/L, potassium dihydrogen phosphate 0.025mol/L, phosphoric acid hydrogen
Disodium 0.08mol/L, ph 7；
(4) mixed liquor is placed on sonicator and carries out brokenly born of the same parents, sonicator frequency 20KHZ, power is
200W, power regulation are adjusted to 80% to 90%, and ultrasonic transformer uses 6 diameters of Φ, and ultrasonic time is worked using 5s, the work that 5s rests
Pattern, net cycle time 200min, final bacterium breakdown ratio are 98.5%.
(5) crush liquid and 15min is centrifuged under 4 DEG C, 6000r/s rotating speeds, collect the extremely broken born of the same parents' enzyme solution of gained supernatant.
(6) treatment fluid 1.5L is prepared, includes urea 0.5mol/L, calcium chloride 0.5mol/L.
Claims (7)
- A kind of 1. method that born of the same parents' enzyme solution treatment fluid reinforcing silty sand is broken using bacterium, which is characterized in that broken using including bacterium Born of the same parents' enzyme solution breaks born of the same parents' enzyme solution treatment fluid with the bacterium of corresponding inorganic consolidating fluid treatment fluid, and multipass is carried out to silty sand sample and repeats to fill Enter processing, it can be by silty sand strength enhancing to 0.5-2Mpa.
- 2. a kind of method that born of the same parents' enzyme solution treatment fluid reinforcing silty sand is broken using bacterium according to claim 1, feature It is, born of the same parents' enzyme solution treatment fluid is broken using the bacterium, silty sand sample is carried out repeating to pour into processing 4-10 times.
- 3. a kind of method that born of the same parents' enzyme solution treatment fluid reinforcing silty sand is broken using bacterium according to claim 1, feature It is, when breaking born of the same parents' enzyme solution treatment fluid using the bacterium and carrying out multipass to silty sand sample and repeat to pour into processing, each Treating capacity is 0.3-2L/m3。
- 4. a kind of method that born of the same parents' enzyme solution treatment fluid reinforcing silty sand is broken using bacterium according to claim 1, feature Be, using the bacterium break born of the same parents' enzyme solution treatment fluid to silty sand sample carry out multipass repeat pour into processing when, adjacent When interval 6-12 is small between reason number.
- A kind of 5. method that born of the same parents' enzyme solution treatment fluid reinforcing desertification soil is broken using bacterium, which is characterized in that break born of the same parents using including bacterium Enzyme solution breaks born of the same parents' enzyme solution treatment fluid with the bacterium of corresponding inorganic consolidating fluid treatment fluid, and sandy land surface is carried out to repeat sprinkling 1-4 It is secondary.
- 6. a kind of method that born of the same parents' enzyme solution treatment fluid reinforcing desertification soil is broken using bacterium according to claim 5, feature are existed In, when breaking born of the same parents' enzyme solution treatment fluid using the bacterium and repeat sprinkling processing to sandy land surface, the place sprayed every time Reason amount is 1-3L/m3。
- 7. a kind of method that born of the same parents' enzyme solution treatment fluid reinforcing desertification soil is broken using bacterium according to claim 5, feature are existed In, using the bacterium break born of the same parents' enzyme solution treatment fluid to sandy land surface carry out repeat sprinkling processing when, adjacent number of processes Between be spaced 6-12h.
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| CN201711237474.8A CN108049390A (en) | 2017-11-30 | 2017-11-30 | A kind of method for breaking born of the same parents' enzyme solution treatment fluid reinforcing silty sand and its soil that desertifies using bacterium |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102677683A (en) * | 2012-04-13 | 2012-09-19 | 水利部交通运输部国家能源局南京水利科学研究院 | Environment-friendly type microorganism-plant combined slope protection method |
| CN105714765A (en) * | 2016-02-01 | 2016-06-29 | 南京林业大学 | Microorganism low-temperature grouting and soil reinforcing method |
| CN106701100A (en) * | 2017-01-09 | 2017-05-24 | 中国神华能源股份有限公司 | Saline soil roadbed filler and method for improving roadbed |
| CN106759248A (en) * | 2016-12-29 | 2017-05-31 | 河海大学 | Barren green desert earth's surface windproof structure |
| CN106947492A (en) * | 2017-02-24 | 2017-07-14 | 河海大学 | A kind of composite sand consolidation model and its preparation method and application |
-
2017
- 2017-11-30 CN CN201711237474.8A patent/CN108049390A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102677683A (en) * | 2012-04-13 | 2012-09-19 | 水利部交通运输部国家能源局南京水利科学研究院 | Environment-friendly type microorganism-plant combined slope protection method |
| CN105714765A (en) * | 2016-02-01 | 2016-06-29 | 南京林业大学 | Microorganism low-temperature grouting and soil reinforcing method |
| CN106759248A (en) * | 2016-12-29 | 2017-05-31 | 河海大学 | Barren green desert earth's surface windproof structure |
| CN106701100A (en) * | 2017-01-09 | 2017-05-24 | 中国神华能源股份有限公司 | Saline soil roadbed filler and method for improving roadbed |
| CN106947492A (en) * | 2017-02-24 | 2017-07-14 | 河海大学 | A kind of composite sand consolidation model and its preparation method and application |
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