CN104630109B - It waste heap reclamation soil microorganism formulation and is reclaimed the method for gangue soil using said preparation - Google Patents
It waste heap reclamation soil microorganism formulation and is reclaimed the method for gangue soil using said preparation Download PDFInfo
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- CN104630109B CN104630109B CN201510062643.3A CN201510062643A CN104630109B CN 104630109 B CN104630109 B CN 104630109B CN 201510062643 A CN201510062643 A CN 201510062643A CN 104630109 B CN104630109 B CN 104630109B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B17/00—Other phosphatic fertilisers, e.g. soft rock phosphates, bone meal
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
Abstract
The invention discloses a kind of waste heap reclamation soil microorganism formulations, it is made of the three kinds of microorganism formulations respectively independently used, wherein contain bacillus subtilis and bacillus licheniformis in I type microorganism formulation, contain frozen glue sample bacillus and bacillus licheniformis in II type microorganism formulation, contain blown-ball Azotobacter, bacillus megaterium, bacillusmusilaginosiengineering and lysine bacillus in III type microorganism formulation, contains effective total viable count (2~200) × 10 in every gram of microorganism formulation8CFU.It is reclaimed gangue soil using mentioned microorganism preparation layering earthing, it is sequentially arranged the first spoil layer from bottom to top, glues loess and flyash mixing overburden layer, the second spoil layer, flyash overburden layer, fine particle spoil layer and loess overburden layer, I type microorganism formulation is spread fertilizer over the fields with flyash mixing overburden layer in viscous loess, flyash overburden layer spreads fertilizer over the fields II type microorganism formulation, loess overburden layer spreads fertilizer over the fields III type microorganism formulation, and crop planting is on loess overburden layer.
Description
Technical field
The invention belongs to waste heap reclamation technical fields, and in particular to a kind of waste heap reclamation soil special microorganism preparation
And the application in Gangue Hill.
Background technique
Gangue is the waste generated in progress of coal mining, accounts for about 20% of coal mining or so.China's coal at present
The comprehensive utilization ratio of spoil is 30~40% or so, stacks nearby mostly as waste, forms hillock not of uniform size, account for
Firming, destroy landscape.Hillock has become that coal field land occupation condition is worst, heap is high-volume maximum, the maximum region of difficulty of governance.
Existing hillock more than 5000 of the whole nation, wherein the spontaneous combustion different there are about 1/3 hillock occurrence degree.The direct shadow of gangue spontaneous combustion
Its soil reclaimation is rung, and causes certain threat to ecological environment.
For the mineralogical composition of gangue based on clay mineral and quartz, chemical composition is more complex, containing carbon, calcium, silicon,
Ten multiple element such as aluminium, iron, magnesium, potassium, alum, boron, nickel, beryllium, in addition to carbon, other elements mostly exist in the form of an oxide, SiO2Contain
Amount nearly 50%.
It is one of the effective measures that hillock is administered by reclaiming to hillock earthing.But since hillock is vertical
Ground condition is poor, and the hillock micro organism quantity after reclaiming is few, soil depletion, and plant growth is difficult, survival rate is low, it is difficult to reach pair
The improvement requirement of environment.In administering method in relation to hillock, as " ZL 200910075216.3 prevents spontaneous combustion of coal gangue hill
Heap storage method ", " 201320524346.2 hillock of ZL is layered earthing pattern of farming ", are mostly the works such as stockpile and store, cover from hillock
Journey measure angle is administered, and great function of the microorganism fungus kind in waste heap reclamation is not accounted for； "ZL
201410251289.4 a kind of combined oxidation inhibitor and amount of application for preventing gangue spontaneous combustion " only account for hillock oneself
Combustion problem, there is no the soil quality problems after consideration waste heap reclamation.
It is generallyd use in production at present by gangue " layering discharge, earthing roll ", then carries out Vegetation Construction, but practice
It proves, this earthing cropping pattern still has many defects: 1) hillock nutrient is barren, and crop growth is difficult；2) cash
Tor ground temperature is high, and soil microbe quantity is few, and microbiologic population is simple, is unfavorable for the accumulation of hillock organic matter；3) hillock
After earthing rolls, oxygen presence is still had, spontaneous combus tion of waste heap is caused.
Summary of the invention
The purpose of the present invention is for main problem existing for waste heap reclamation, provide a kind of waste heap reclamation soil with micro-
Biological agent, and reclaimed the method for hillock soil using the microorganism formulation.
Waste heap reclamation soil of the present invention with microorganism formulation by respectively independently use I type microorganism formulation,
Three kinds of microorganism formulations of II type microorganism formulation and III type microorganism formulation are constituted.
Wherein:
I type microorganism formulation is made of absorption carrier and the effective viable bacteria being adsorbed on the absorption carrier, described effective
Viable bacteria is made of bacillus subtilis and bacillus licheniformis, and the living bacteria count of bacillus subtilis and bacillus licheniformis
Ratio is 1: 1~1.5；
II type microorganism formulation is by absorption carrier, active additive and the effective viable bacteria group being adsorbed on the absorption carrier
At effective viable bacteria is made of bacillusmusilaginosiengineering and bacillus licheniformis, and bacillusmusilaginosiengineering and lichens gemma
The living bacteria count ratio of bacillus is 1: 1~1.5；The active additive is calcium magnesium phosphate, and additive amount accounts for II type microorganism system
8~15wt% of agent total amount；
III type microorganism formulation is by absorption carrier, active additive and the effective viable bacteria group being adsorbed on the absorption carrier
At, effective viable bacteria is made of blown-ball Azotobacter, bacillus megaterium, bacillusmusilaginosiengineering and lysine bacillus,
And blown-ball Azotobacter, bacillus megaterium, bacillusmusilaginosiengineering and the living bacteria count ratio of lysine bacillus are 1: 1
~1.5: 2~2.5: 1~2；The active additive be calcium magnesium phosphate, additive amount account for III type microorganism formulation total amount 8~
15wt%；
Meanwhile in three kinds of microorganism formulations described above, the effective total viable count contained in every gram of microorganism formulation is
(2~200) × 108CFU。
Microorganism fungus kind involved in microorganism formulation of the present invention has six kinds, respectively bacillus subtilis
(Bacillus subtilis) ACCC10242, lichens gemma bar (Bacillus licheniformis) ACCC01193, it is huge
Bacterium anthracoides (Bacillus megaterium) ACCC11107, bacillusmusilaginosiengineering (Bacillus
Mucilaginosus) ACCC10013, blown-ball Azotobacter (Azotobacter chroococcum) ACCC10006, spindle
Lysine bacillus (Lysinibacillus fusiformis) CGMCC1.3602, is freeze-drying lactobacillus, is commercially available production
Product.
Specifically, in microorganism formulation of the invention, the effective viable bacteria being adsorbed on absorption carrier is by described micro-
The bacterium mud that biological inoculum fermentation liquid centrifugation obtains.
In the present invention, the absorption carrier is one of diatomite, bentonite, straw powder, sawdust or turfy soil, or
Several arbitrary proportion mixtures.
More specifically, the absorption carrier in the I type microorganism formulation and II type microorganism formulation be preferably diatomite and/
Or bentonite；Absorption carrier in the III type microorganism formulation is preferably one of straw powder, sawdust or turfy soil or several
The arbitrary proportion mixture of kind.
It is reclaimed the method for gangue soil the present invention also provides a kind of using mentioned microorganism preparation, is by hillock point
Layer earthing, and spreads fertilizer over the fields the microorganism formulation in the overburden layer, and the gangue soil of reclaiming is followed successively by the from bottom to top
One spoil layer glues loess and flyash mixing overburden layer, the second spoil layer, flyash overburden layer, fine particle spoil layer, loess
Overburden layer spreads fertilizer over the fields I type microorganism formulation with flyash mixing overburden layer in the viscous loess, and the flyash overburden layer spreads fertilizer over the fields II
Type microorganism formulation, the loess overburden layer spread fertilizer over the fields III type microorganism formulation, and crop planting is on loess overburden layer.
Wherein, the amount of spreading fertilizer over the fields of I type, II type and III type microorganism formulation on respective overburden layer is to apply 5 per acre
~10kg.
In gangue soil method of the present invention of reclaiming, the laying depth of the first spoil layer is generally 6~10m, the
The laying depth of two spoil layers is 3~5m, 1~3m of fine particle spoil layer laying depth.
In turn, viscous loess, the flyash overburden layer being covered on the first spoil layer are by viscous loess and flyash according to 1:
0.6~1 mass ratio, which mixes, to be constituted, and the overburden layer with a thickness of 20~40cm, after mechanical ramming, spread fertilizer over the fields the micro- life of I type
Object preparation；Be covered on the flyash overburden layer on the second spoil layer with a thickness of 20~40cm, after mechanical ramming, it is micro- to spread fertilizer over the fields II type
Biological agent；The loess overburden layer of top layer is then made of the loess of 40~80cm thickness, row replacement or spreads fertilizer over the fields III type microorganism formulation.
Waste heap reclamation soil of the invention is made of with microorganism formulation three kinds of different microorganism formulations, in hillock
Different microorganism formulations is applied during earthing, by introducing purpose microorganism fungus kind, can effectively solve the problems, such as hillock.
Hillock is tentatively smooth, viscous loess and flyash are covered after compacting as earthing first layer, after cal rolling
I type microorganism formulation is spread fertilizer over the fields, the bacillus subtilis and bacillus licheniformis of introducing are aerobic, high temperature resistant strain, can be consumed
Oxygen, isolation fresh air conveys oxygen to deep spoil, and has blocked the flyway of high temperature heat source and flue gas to earth's surface, leads to
Cross effectively prevent oxygen transport downwards and flue gas discharge upwards, prevention the spontaneous combustion of hillock bottom generation.Top re-forms spoil
Stack layer covers flyash as the earthing second layer, II type microorganism formulation is spread fertilizer over the fields after mechanical ramming, is introduced after smooth compacting
Aerobic bacillus licheniformis resistant to high temperature can both consume oxygen, prevent outside air and feed to spoil layer lower part, while also can
In conjunction with potassium solubilizing bacteria bacillusmusilaginosiengineering, accelerate the further weathering of upper layer fine particle spoil layer together, calcium magnesium phosphate can have
Effect reduces the activity of fine particle spoil layer heavy metal, prevents the migration of heavy metal crop to the ground.On be fine particle cash
Rock layers cover the loose loess formation of shallow-layer of 40~80cm on the fine particle spoil layer of surface layer, and applying some has phosphorus decomposing, potassium decomposing, solid
The strain of nitrogen growth-promoting functions can accelerate the weathering of spoil layer, activate and efficiently use a large amount of existing K and Si in gangue,
The nutrient content for improving topsoil (weathered layer), promotes the growth of crops, increases the ground mulching degree of hillock.In addition,
The activators such as some calcium magnesium phosphate are added, heavy metal also can be effectively held, weaken its murder by poisoning to crops.
The various microorganism formulations of the present invention all have advantage easy to operate, easy to use, low in cost, that activation rate is high.
Present invention combination hillock is layered earthing technology, by the way that three kinds of microorganism formulations are administered in combination, can improve supporting for hillock
Divide situation, promote the growth of crops on hillock, while the validity of hillock heavy metal can be reduced again, accelerates surface layer tiny
The weathering of spoil particle, additionally it is possible to prevent spontaneous combus tion of waste heap from the root, effectively solve the spontaneous combustion problems of hillock.
Specific embodiment
Embodiment 1
Preparing microorganism fungus kind involved in microorganism formulation has six kinds, respectively bacillus subtilis (Bacillus
Subtilis) ACCC10242, lichens gemma bar (Bacillus licheniformis) ACCC01193, bacillus megaterium
(Bacillus megaterium) ACCC11107, bacillusmusilaginosiengineering (Bacillus mucilaginosus)
ACCC10013, blown-ball Azotobacter (Azotobacter chroococcum) ACCC10006, spindle lysine bacillus
(Lysinibacillus fusiformis) CGMCC1.3602, is freeze-drying lactobacillus, is commercial products.
Purpose strain is aseptically successively activated respectively, inclined-plane culture, liquid shaking bottle and fermentation tank culture.
1) it activates.Under aseptic condition, by the peace unstrained spirits pipe of microorganism fungus kind is saved equipped with freeze-drying to be soaked with the de- of 75% alcohol
Rouge cotton rub is net, the top of peace unstrained spirits pipe is heated with flame, dripping a small amount of sterile water to heated tip makes its rupture, knocks rupture open with tweezers
Peace unstrained spirits pipe.The suitable fluid nutrient medium of 0.3~0.5mL is drawn with aseptic straw, is instilled in peace unstrained spirits pipe, is gently vibrated, make to freeze
Dry mycelium dissolution is in suspension.Whole thallus suspension liquids are drawn, are transplanted in 2 slant medium test tubes respectively, in suggestion
At a temperature of cultivate activation, with good growth and breeding ability.
2) inclined-plane culture.Activated purpose strain is stored on slant medium using method of scoring, 30 DEG C of cultures 3
~5 days.Bacillus subtilis, bacillus licheniformis, bacillus megaterium and lysine bacillus select nutrient to train
Support base: peptone 10g, NaCl 5g, beef extract 5g, agar 15g, distilled water 1000mL, pH7.0.Gel-shaped bacillus is selected
Silicate bacteria culture medium: sucrose 10g, yeast extract 0.4g, K2HPO40.5g, CaCO31g, MgSO4·7H2O 0.2g, FeCl3
0.005g, agar 18g, distilled water 1000mL, pH7.0~7.2.Blown-ball Azotobacter select nitrogen-fixing bacteria agar medium: sucrose or
Mannitol 10g, K2HPO4·3H2O 0.5g, NaCl 0.2g, CaCO3 1g, MgSO4·7H2O 0.2g, 15~20g of agar steam
Distilled water 1000mL, pH7.0~7.2.
3) liquid shaking bottle, fermentation tank culture expand.Bacillus subtilis, bacillus licheniformis, bacillus megaterium and rely
The fermented and cultured process of propylhomoserin bacillus is: the strain being stored on inclined-plane after activation is connected to equipped with 100mL nutrient
In the 250mL shaking flask of culture medium, 30 DEG C, 170r/min shaking flask culture 18~for 24 hours, obtain seed liquor；By volume by seed liquor
In the fermentation medium of 10% inoculum concentration access seeding tank, 30 DEG C, 180r/min fermented and cultured 20~for 24 hours, obtain fermentation seed
Liquid, then by fermentation seed liquid by volume 10% inoculum concentration access fermentor fermentation medium in, 30 DEG C, 180r/min
36~48h of fermented and cultured, obtains zymocyte liquid；Fermentation medium in seeding tank and fermentor is beef extract 5g/L, peptone
5g/L, pH7.0,121 DEG C of 5g/L, NaCl real tanks sterilizing 25min.The fermented and cultured process of gel-shaped bacillus is: will activate
The strain being stored on inclined-plane afterwards is connected in the 250mL shaking flask equipped with 100mL silicate bacteria culture medium, 30 DEG C, 170r/min
Shaking flask culture 18~for 24 hours, obtain seed liquor；By seed liquor by volume 10% inoculum concentration access seeding tank fermentation medium
In, 30 DEG C, 180r/min fermented and cultured 22~for 24 hours, fermentation seed liquid is obtained, then by fermentation seed liquid by volume 10%
Inoculum concentration accesses in the fermentation medium of fermentor, and 30 DEG C, 180r/min 36~48h of fermented and cultured obtain zymocyte liquid；Seed
Fermentation medium in tank and fermentor is sucrose 10g/L, yeast extract 0.4g/L, K2HPO40.5g/L, CaCO31g/L,
MgSO4·7H2O 0.2g/L, FeCl30.005g/L, pH7.0~7.2,121 DEG C of real tanks sterilizing 25min.The hair of blown-ball Azotobacter
Ferment incubation is: the strain being stored on inclined-plane after activation is connected to the 250mL shaking flask equipped with 100mL fixed nitrogen bacterium culture medium
In, 30 DEG C, 170r/min shaking flask culture 16~for 24 hours, obtain seed liquor；By seed liquor by volume 10% inoculum concentration access kind
In the fermentation medium of sub- tank, 30 DEG C, 180r/min fermented and cultured 16~for 24 hours, fermentation seed liquid is obtained, then by fermentation seed
By volume in the fermentation medium of 10% inoculum concentration access fermentor, 30 DEG C, 180r/min 36~48h of fermented and cultured are obtained liquid
To zymocyte liquid；Fermentation medium in seeding tank and fermentor is sucrose 10g/L, K2HPO4·3H2O 0.5g/L, NaCl
0.2g/L, CaCO31g/L, MgSO4·7H20.2g/L, pH7.0~7.2,121 DEG C of O real tanks sterilizing 25min.
It adjusts bacteria concentration in the above-mentioned various fermentation liquids fermented and reaches 1 × 109~1 × 1011CFU/mL, by various fermentations
Liquid at 20~30 DEG C, under the conditions of 3500~7000rpm be centrifuged 20~40min, centrifugal sediment with sterile water washing 1~5 time, then
Secondary centrifugation, centrifugal sediment are the bacterium mud of each strain.
Embodiment 2
Bacillus subtilis bacterium mud 50g, Bacillus licheniformis mud 50g are weighed, it is mixed with diatomite 150g, bentonite 150g
It closes, blender at the uniform velocity stirs evenly, and aeration-drying in 15~50 DEG C of sterile draft chamber is placed in, until water content < 10%, obtains I
Type microorganism formulation, living bacteria count > 2 × 108CFU/g。
Bacillusmusilaginosiengineering bacterium mud 50g, Bacillus licheniformis mud 75g are weighed, with diatomite 175g, bentonite 200g
Calcium magnesium phosphate 75g is added in mixing, and blender at the uniform velocity stirs evenly, and is placed in aeration-drying in 15~50 DEG C of sterile draft chamber, until
Water content < 10% obtains II type microorganism formulation, living bacteria count > 2 × 108CFU/g。
It weighs blown-ball Azotobacter bacterium mud 25g, bacillus megaterium bacterium mud 25g, bacillusmusilaginosiengineering bacterium mud 50g, rely ammonia
Sour bacillus bacterium mud 25g is mixed with straw powder 150g, sawdust 100g, turfy soil 125g, then is added calcium magnesium phosphate 85g, stirring
Device at the uniform velocity stirs evenly, and is placed in aeration-drying in 15~50 DEG C of sterile draft chamber, until water content < 10%, obtains the micro- life of III type
Object preparation, living bacteria count > 2 × 108CFU/g。
Embodiment 3
Bacillus subtilis bacterium mud 50g, Bacillus licheniformis mud 75g are weighed, it is mixed with diatomite 200g, bentonite 175g
It closes, blender at the uniform velocity stirs evenly, and aeration-drying in 15~50 DEG C of sterile draft chamber is placed in, until water content < 10%, obtains I
Type microorganism formulation, living bacteria count > 2 × 108 CFU/g。
Bacillusmusilaginosiengineering bacterium mud 75g, Bacillus licheniformis mud 75g are weighed, with diatomite 200g, bentonite 250g
Calcium magnesium phosphate 90g is added in mixing, and blender at the uniform velocity stirs evenly, and is placed in aeration-drying in 15~50 DEG C of sterile draft chamber, until
Water content < 10% obtains II type microorganism formulation, living bacteria count > 2 × 108CFU/g。
It weighs blown-ball Azotobacter bacterium mud 50g, bacillus megaterium bacterium mud 75g, bacillusmusilaginosiengineering bacterium mud 125g, rely ammonia
Sour bacillus bacterium mud 100g is mixed with straw powder 350g, sawdust 350g, turfy soil 350g, then is added calcium magnesium phosphate 250g, is stirred
It mixes device at the uniform velocity to stir evenly, is placed in aeration-drying in 15~50 DEG C of sterile draft chamber, until water content < 10%, it is micro- to obtain III type
Biological agent, living bacteria count > 2 × 108CFU/g。
Application examples 1
When hillock is stacked, first after the first spoil layer that lowest part laying depth is 6m, mechanical ramming, it is laid with one layer
The 20cm thick overburden layer mixed by gluing loess and flyash in 1: 0.6 ratio, mechanical ramming spread fertilizer over the fields 5kg embodiment 2 per acre
I type microorganism formulation.The second spoil layer of top ulking thickness 3m again accumulates the fine coal after 20cm on spoil layer after compacting
Grieshoch after mechanical ramming, spreads fertilizer over the fields II type microorganism formulation of 6kg embodiment 2 per acre.Cover the fine particle spoil of 1m again later
Layer, mechanical ramming covers the loose loess formation of 80cm, spreads fertilizer over the fields on loess formation or III type microorganism formulation of row replacement embodiment 2, uses
Amount is 9kg per acre, and plants crops.
Application examples 2
When hillock is stacked, first after the first spoil layer that lowest part laying depth is 8m, mechanical ramming, it is laid with one layer
The 30cm thick overburden layer mixed by gluing loess and flyash in 1: 1 ratio, mechanical ramming spread fertilizer over the fields 8kg embodiment 3 per acre
I type microorganism formulation.The second spoil layer of top ulking thickness 5m again accumulates the flyash after 30cm on spoil layer after compacting
Layer, after mechanical ramming, II type microorganism formulation of 10kg embodiment 3 is spread fertilizer over the fields per acre.Cover the fine particle spoil of 2m again later
Layer, mechanical ramming covers the loose loess formation of 60cm, spreads fertilizer over the fields on loess formation or III type microorganism formulation of row replacement embodiment 3, uses
Amount is 10kg per acre, and plants crops.
Claims (9)
- It is that hillock is layered earthing 1. being reclaimed the method for gangue soil using waste heap reclamation soil with microorganism formulation, And microorganism formulation is spread fertilizer over the fields in the overburden layer；The gangue soil of reclaiming is followed successively by the first spoil layer from bottom to top, glues Loess and flyash mixing overburden layer, the second spoil layer, flyash overburden layer, fine particle spoil layer, loess overburden layer；It is described Microorganism formulation is made of the I type microorganism formulation, II type microorganism formulation and III type microorganism formulation respectively independently used, The viscous loess spreads fertilizer over the fields I type microorganism formulation with flyash mixing overburden layer, and flyash overburden layer spreads fertilizer over the fields II type microorganism formulation, Loess overburden layer spreads fertilizer over the fields III type microorganism formulation, and crop planting is on loess overburden layer；Wherein:I type microorganism formulation is made of absorption carrier and the effective viable bacteria being adsorbed on the absorption carrier, effective viable bacteria It is made of bacillus subtilis and bacillus licheniformis, the living bacteria count ratio of the bacillus subtilis and bacillus licheniformis Example is 1: 1~1.5；II type microorganism formulation is made of absorption carrier, active additive and the effective viable bacteria being adsorbed on the absorption carrier, Effective viable bacteria is made of bacillusmusilaginosiengineering and bacillus licheniformis, the bacillusmusilaginosiengineering and lichens gemma bar The living bacteria count ratio of bacterium is 1: 1~1.5, and the active additive is calcium magnesium phosphate, and additive amount accounts for II type microorganism formulation 8~15wt% of total amount；III type microorganism formulation is made of absorption carrier, active additive and the effective viable bacteria being adsorbed on the absorption carrier, Effective viable bacteria is made of blown-ball Azotobacter, bacillus megaterium, bacillusmusilaginosiengineering and lysine bacillus, described Blown-ball Azotobacter, bacillus megaterium, bacillusmusilaginosiengineering and the living bacteria count ratio of lysine bacillus be 1: 1~ 1.5: 2~2.5: 1~2, the active additive be calcium magnesium phosphate, additive amount account for III type microorganism formulation total amount 8~ 15wt%；In three of the above microorganism formulation, the effective total viable count contained in every gram of microorganism formulation is (2~200) × 108CFU。
- 2. the method for gangue soil according to claim 1 of reclaiming, it is characterized in that described be adsorbed on absorption carrier Effective viable bacteria is the bacterium mud obtained by the microorganism fungus kind fermentation liquid centrifugation.
- 3. the method for gangue soil according to claim 1 of reclaiming, it is characterized in that the absorption carrier be diatomite, One of bentonite, straw powder, sawdust or turfy soil or several arbitrary proportion mixtures.
- 4. the method for gangue soil according to claim 3 of reclaiming, it is characterized in that the I type microorganism formulation and II type Absorption carrier in microorganism formulation is diatomite and/or bentonite.
- 5. the method for gangue soil according to claim 3 of reclaiming, it is characterized in that in the III type microorganism formulation Absorption carrier is one of straw powder, sawdust or turfy soil or several arbitrary proportion mixtures.
- 6. the method for gangue soil according to claim 1 of reclaiming, it is characterized in that I type, II type and the micro- life of III type Spread fertilizer over the fields amount of the object preparation on respective overburden layer is to apply 5~10kg per acre.
- 7. the method for gangue soil according to claim 1 of reclaiming, it is characterized in that the viscous loess, flyash earthing Layer is to be mixed to constitute according to 1: 0.6~1 mass ratio with flyash by viscous loess, 20~40cm of the overburden layer thickness.
- 8. the method for gangue soil according to claim 1 of reclaiming, it is characterized in that the flyash overburden layer thickness 20~40cm.
- 9. the method for gangue soil according to claim 1 of reclaiming, it is characterized in that the loess overburden layer by 40~ The loess of 80cm thickness is constituted.
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