CN107100162A - A kind of solid indigenous method of microorganism gas-liquid cycle slip casting - Google Patents

A kind of solid indigenous method of microorganism gas-liquid cycle slip casting Download PDF

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Publication number
CN107100162A
CN107100162A CN201710371199.2A CN201710371199A CN107100162A CN 107100162 A CN107100162 A CN 107100162A CN 201710371199 A CN201710371199 A CN 201710371199A CN 107100162 A CN107100162 A CN 107100162A
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soil body
solid
slip casting
treating
solid soil
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CN201710371199.2A
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CN107100162B (en
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邵光辉
冯建挺
赵志峰
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Nanjing Forestry University
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Nanjing Forestry University
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    • E—FIXED CONSTRUCTIONS
    • E02—HYDRAULIC ENGINEERING; FOUNDATIONS; SOIL SHIFTING
    • E02D—FOUNDATIONS; EXCAVATIONS; EMBANKMENTS; UNDERGROUND OR UNDERWATER STRUCTURES
    • E02D3/00—Improving or preserving soil or rock, e.g. preserving permafrost soil
    • E02D3/12—Consolidating by placing solidifying or pore-filling substances in the soil

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  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Structural Engineering (AREA)
  • Agronomy & Crop Science (AREA)
  • Environmental & Geological Engineering (AREA)
  • Soil Sciences (AREA)
  • General Life Sciences & Earth Sciences (AREA)
  • Mining & Mineral Resources (AREA)
  • Paleontology (AREA)
  • Civil Engineering (AREA)
  • General Engineering & Computer Science (AREA)
  • Consolidation Of Soil By Introduction Of Solidifying Substances Into Soil (AREA)

Abstract

A kind of solid indigenous method of microorganism gas-liquid cycle slip casting, step includes：A. grouting tube 1 is vertically squeezed into ground 7；B. by grouting tube 1 to treating to inject the OD for treating solid 1.5 times of 8 pore volume of the soil body in the solid soil body 8600=1.2 bacterium solution 3, reinjects urea and CaCl for 0.5mol/L~1.0mol/L with the isometric concentration of bacterium solution 32The consolidating fluid 4 mixed；C. make to treat that the solid soil body 8 is in unsaturated state, stand 12h to treating that it is that 100KPa~200KPa, volume are to treat the compressed air of solid 1.5 times of soil body pore volume that pressure is injected in the solid soil body 8, and the Pore Solution treated in the solid soil body 8 is ejected using air compressor 6；D. to treating to inject 1.5 times of consolidating fluids 4 for treating the solid pore volume of the soil body 8 in the solid soil body 8,12h is stood；E. d is repeated to operate 1 time；F. b, c, d, e operation is repeated, until Dai Gu soil bodys Final 8 degree reaches that requirement or slip casting liquid (bacterium solution 3 or consolidating fluid 4) can not be injected when in the solid soil body 8, stops slip casting.

Description

A kind of solid indigenous method of microorganism gas-liquid cycle slip casting
Technical field
It is apt to soil strength uniformity the present invention relates to the solid land reform of microorganism gas-liquid cycle slip casting and improves grouting process enhancing The method of soil strength, belongs to the technical field of soil stabilization.
Background technology
Microorganism soil-fixing technology is ecological, the environmentally friendly solid indigenous method of a class, and the solid soil of existing microorganism slip casting mainly passes through Microbial inoculum and consolidating fluid are alternately injected, using microorganism induction calcium carbonate crystal technology (MICP technologies), finally in soil The cementing soil particle of calcium carbonate crystal is separated out in intergranular pore.The CaCO of microorganism induction deposition3Crystalline solid has cementing with filling Effect, the soil body can be reinforced effectively.It is main in current engineering to utilize microorganism slip casting solidification sand and silt.
The technical scheme of prior art one
It is that low speed injects a certain amount of microbial inoculum into soil first, then low speed injects the CaCl of low concentration2It is fixed Liquid, utilizes Ca2+Flocculation, realize the fixation of microorganism, last low speed injection consolidating fluid (i.e. urea and CaCl2Mixing Liquid) so that the continuous mineralising generation calcite crystalline solid of microorganism in sand particle surface is adsorbed, solidifies the soil body.
The shortcoming of prior art one
CaCO is separated out for control reaction3The speed of crystalline solid, microorganism slip casting solidification sand, which is used, alternately injects micro- Biological bacterium solution and consolidating fluid, grouting method length change amplitude along along grouting serous fluid flow direction are big, there is significant inequality CaCO near even property, grouting port3Crystalline solid obstruction soil body hole formation is blocked, and causes distal end to reinforce difficult, single hole slip casting is effective Reinforcing scope is small, and this method cycle is long, and efficiency is low, and stock utilization is low, deficiency in economic performance.
The technical scheme of prior art two
Microbial inoculum stirring is buried, is made after sample and stands a period of time, bacterium is fully adsorbed, then repeatedly injection Consolidating fluid so that the continuous mineralising of microorganism of the absorption on soil particle separates out calcite crystalline solid, solidifies the soil body.
The shortcoming of prior art two
Because being buried using microbial inoculum stirring, so the grouting method can only once introduce microorganism to the soil body is reinforced Bacterium, as multiple consolidating fluid injects, micro organism quantity consumption decay in the soil body, and the efficiency of calcium carbonate that its mineralising is produced is very Fast reduction, it is difficult to form the soil mass consolidation of high intensity.
The technical scheme of prior art three
Mix microbial inoculum and consolidating fluid respectively in soil, sample is made in uniform mixing, and stand makes the soil body for a period of time Form intensity.
The shortcoming of prior art three
Microbial inoculum and consolidating fluid are mixed and stirred during soil sample, and new adhesive bond generation, original adhesive bond is destroyed, Soil body final strength increase rate is small, and Practical Project poor performance, engineering application value is small.
The technical scheme of prior art four
The soil sample being made is directly immersed in binding liquid to (binding liquid is except mainly containing urea and CaCl2In addition, also contain There is NH4Cl、NaHCO3And nutriment), a period of time is stood, by binding liquid free permeation into soil body hole, with micro- life PH value rise in thing metabolic process, the cementing soil body of microorganism induction calcium carbonate crystal.
The shortcoming of prior art four
Microorganism adsorption is on soil particle in the testing program, by mineralization, adjusts pH, makes the carbonate knot of generation Partial crystallization goes out, and reaction is slow, Gu the native cycle is long.
The content of the invention
Technical problem：Existing microorganism slip casting soil retaining technology, the slip casting cycle is longer, and efficiency is low, and stock utilization is low；Slip casting Soil body permeability is poor near mouthful, easily block, and causes distal end to reinforce difficulty, it is difficult to form the uniform consolidation body of high intensity, single hole note Starch effective reinforced area small.The purpose of the present invention is the gas using alternately injection microbial inoculum, compressed air and consolidating fluid Liquid circulates slip casting soil retaining technology, and the saturation degree for treating the solid soil body is reduced by injecting compressed air, realizes in low CaCO3Crystallization content Under the conditions of form high intensity, save material usage, while solving the problem of grouting port distal end is reinforced, expand single hole slip casting and effectively add Gu scope.
Technical scheme
The present invention principle be：Unsaturation effect using the solid soil of microorganism slip casting overcomes lacking for existing saturation slip casting presence Fall into.During the solid soil of microorganism slip casting under soil body saturation state, CaCO3Soil is mainly filled in its effect when crystallization content is relatively low Based on body opening gap, it is cementing supplemented by, soil strength improve it is limited；In CaCO3Its effect is mainly filled under crystallization content moderate condition Soil body hole is to be played a role jointly with cementing soil particle；In CaCO3Under crystallization content higher strip part, CaCO is increased newly3Crystallization is to the soil body The raising contribution efficiency of intensity is significantly improved, but the now close closing of soil body hole, it is impossible to continue slip casting.In soil body unsaturation shape During the solid soil of microorganism slip casting under state, the CaCO that microorganism induction is separated out3Easily calcite is formed on soil body particle surface Coating, and the preferential precipitation calcium carbonate crystal body at soil particle contact point, form bridge joint between soil particle, are analysed under unsaturated state The CaCO gone out3Crystalline solid is mainly used in cementing soil particle, only minimal amount of CaCO3Crystalline solid fills soil body hole, makes to be reinforced The soil body is in low CaCO3With regard to high intensity can be formed under the conditions of crystallization content, in high CaCO3Crystallization content condition still keeps high osmosis, protects The soil body property noted is held, prevents from blocking near grouting port, it is ensured that the grouting port distal end soil body is reinforced, expands single hole slip casting and effectively add Gu scope.
The solid indigenous method of a kind of microorganism gas-liquid cycle slip casting, it is characterised in that this method step is as follows：
A. grouting tube 1 is vertically squeezed into ground 7；
B. by grouting tube 1, first to treating that the concentration for treating solid 1.5 times of soil body pore volume is injected in the solid soil body 8 is OD600=1.2 bacterium solution 3, reinjects urea and CaCl for 0.5mol/L~1.0mol/L with the isometric concentration of bacterium solution 32It is mixed The consolidating fluid 4 closed；
C. it is 100KPa~200KPa, volume to treat the solid soil body to treating to inject pressure in the solid soil body 8 using air compressor 6 The compressed air of 8 1.5 times of pore volumes, the Pore Solution treated in the solid soil body 8 is ejected, and makes to treat that the solid soil body 8 is in unsaturated state, Stand 12h；
D. to treating to inject the consolidating fluid 4 for treating solid 1.5 times of 8 pore volume of the soil body in the solid soil body 8,12h is stood；
E. d is repeated to operate 1 time；
F. b, c, d, e operation is repeated, (bacterium solution 3 is cementing until Dai Gu soil bodys Final 8 degree reaches requirement or slip casting liquid Liquid 4) it can not inject when in the solid soil body 8, stop slip casting.
The method of the solid soil of described microorganism gas-liquid cycle slip casting, it is characterised in that injecting compressed air makes soil in step c Body is in unsaturated state, and saturation degree need to be controlled 20%~40%.
The method of the solid soil of described microorganism gas-liquid cycle slip casting, it is characterised in that bacterium solution used is Bacillus pasteurii (S.pasteurii)。
Beneficial effects of the present invention：
(1) the gas-liquid cycle grouting and reinforcing soil body effectively improves the uniformity of soil stabilization, prevents from blocking near grouting port, Ensure that the grouting port distal end soil body is reinforced, expand single hole slip casting effective reinforced area.
(2) it ensure that during the slip casting of microorganism gas-liquid cycle and treat that the solid soil body keeps original permeability not drop significantly It is low, many wheel slip castings to fine grained soil can be achieved, very high intensity can also be reached by microorganism slip casting is solidified fine grained soil, extend microorganism Gu the applicable soil property scope of soil.
(3) identical CaCO is being produced3Higher soil strength can be obtained under content, injecting paste material consumption is reduced, improves Stock utilization.
Brief description of the drawings
Fig. 1 slip casting schematic diagrames
Reference：1- grouting tubes, 2- grouting pumps, 3- bacterium solutions, 4- consolidating fluids, 5- valves, 6- air compressors, 7- Base, 8- treats the solid soil body
Embodiment
Embodiment 1
In this example, a kind of solid indigenous method of microorganism gas-liquid cycle slip casting is used to solidifying sand foundation, this method include with Lower step：
A. grouting tube 1 is vertically squeezed into ground 7；
B. by grouting tube 1, first to treating that the concentration for treating solid 1.5 times of soil body pore volume is injected in the solid soil body 8 is OD600=1.2 Bacillus pasteurii bacterium solution 3, reinject with the isometric concentration of bacterium solution 3 for 1.0mol/L urea with 1.0mol/L CaCl2The consolidating fluid 4 mixed；
C. it is 100KPa, volume to treat solid soil body pore volume to treating to inject pressure in the solid soil body 8 using air compressor 6 1.5 times of compressed air, the Pore Solution treated in the solid soil body 8 is ejected, and is made to treat that the solid soil body 8 is in unsaturated state, is stood 12h；
D. to treating to inject the consolidating fluid 4 for treating solid 1.5 times of soil body pore volume in the solid soil body 8,12h is stood；
E. d is repeated to operate 1 time；
F. b, c, d, e operation is repeated, (bacterium solution 3 is cementing until Dai Gu soil bodys Final 8 degree reaches requirement or slip casting liquid Liquid 4) it can not inject when in the solid soil body 8, stop slip casting.
Embodiment 2
In this example, a kind of solid indigenous method of microorganism gas-liquid cycle slip casting is used to solidifying silt foundation, this method include with Lower step：
A. grouting tube 1 is vertically squeezed into ground 7；
B. by grouting tube 1, first to treating that the concentration for treating solid 1.5 times of soil body pore volume is injected in the solid soil body 8 is OD600=1.2 Bacillus pasteurii bacterium solution 3, reinject with the isometric concentration of bacterium solution 3 for 0.5mol/L urea with 0.5mol/L CaCl2The consolidating fluid 4 mixed；
C. it is 200KPa, volume to treat the solid pore-body of the soil body 8 to treating to inject pressure in the solid soil body 8 using air compressor 6 The compressed air of 1.5 times of product, the Pore Solution treated in the solid soil body 8 is ejected, and is made to treat that the solid soil body 8 is in unsaturated state, is stood 12h；
D. to treating to inject the consolidating fluid 4 for treating solid 1.5 times of 8 pore volume of the soil body in the solid soil body 8,12h is stood；
E. d is repeated to operate 1 time；
F. b, c, d, e operation is repeated, (bacterium solution 3 is cementing until Dai Gu soil bodys Final 8 degree reaches requirement or slip casting liquid Liquid 4) it can not inject when in the solid soil body 8, stop slip casting.

Claims (3)

1. the solid indigenous method of a kind of microorganism gas-liquid cycle slip casting, it is characterised in that this method step is as follows：
A. grouting tube (1) is vertically squeezed into ground (7)；
B. to treating to inject the concentration for treating 1.5 times of the solid soil body (8) pore volume in the solid soil body (8) for OD600=1.2 bacterium solution (3), Reinject urea and CaCl for 0.5mol/L~1.0mol/L with the isometric concentration of bacterium solution (3)2The consolidating fluid mixed (4)；
C. using air compressor (6) to treating in the solid soil body (8) that injection pressure is 100KPa~200KPa, volume to treat the solid soil body (8) compressed air of 1.5 times of pore volume, the Pore Solution treated in the solid soil body (8) is ejected, and makes to treat the solid soil body (8) in non-full And state, stand 12h；
D. to treating to inject the consolidating fluid (4) for treating 1.5 times of the solid soil body (8) pore volume in the solid soil body (8), 12h is stood；
E. d is repeated to operate 1 time；
F. b, c, d, e operation is repeated, until treating that the solid soil body (8) intensity reaches requirement or slip casting liquid (bacterium solution (3) or cementing Liquid (4)) it can not inject when in the solid soil body (8), stop slip casting.
2. the method for the solid soil of microorganism gas-liquid cycle slip casting according to claim 1, it is characterised in that pressure is injected in step c Contracting air makes the soil body be in unsaturated state, and saturation degree need to be controlled 20%~40%.
3. the method for the solid soil of microorganism gas-liquid cycle slip casting according to claim 1, it is characterised in that bacterium solution used is bar Family name bacillus (S.pasteurii).
CN201710371199.2A 2017-05-19 2017-05-19 Indigenous method is consolidated in a kind of microorganism gas-liquid cycle slip casting Active CN107100162B (en)

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Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108049409A (en) * 2017-11-14 2018-05-18 河海大学 A kind of method of the solid soil of microbes synthase inhibitor slip casing by pressure
CN108192623A (en) * 2018-01-10 2018-06-22 南京林业大学 Bayer process red mud roadbed microorganism curing and application process
CN108220197A (en) * 2018-01-10 2018-06-29 南京林业大学 A kind of gold tailings slag microorganism combined processing and application process
CN109165479A (en) * 2018-10-29 2019-01-08 西南大学 The soil body is applicable in the judgment method of MICP technology
CN110130318A (en) * 2019-05-24 2019-08-16 南京林业大学 A kind of construction method of microorganism mixing-filling stake
CN110284491A (en) * 2019-07-31 2019-09-27 南京林业大学 A kind of magnetized solution microorganism soil-fixing device and solid indigenous method
CN110886282A (en) * 2019-12-17 2020-03-17 西南石油大学 Particle excitation type microorganism soil stabilization wall protection device
CN112030942A (en) * 2020-08-31 2020-12-04 南京林业大学 Method for improving liquefaction resistance of foundation by silicate mineral decomposing bacteria
CN114396038A (en) * 2022-01-21 2022-04-26 中煤航测遥感集团有限公司 Method for reinforcing soil strength by using microorganism induced calcium carbonate deposition
CN115247420A (en) * 2022-09-02 2022-10-28 浙江大学 Calcareous sand MICP pile based on prefabricated biological pulp and construction method thereof
CN116335115A (en) * 2023-02-21 2023-06-27 广州航海学院 Liquefied sand foundation treatment method combining high-energy gas fracturing and MICP technology
CN116397618A (en) * 2023-02-21 2023-07-07 广州航海学院 Method for reinforcing foundation of silt by combining hydraulic fracturing and MICP technology

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CN105507232A (en) * 2015-12-08 2016-04-20 南京林业大学 Microorganism slab grouting device and method used for reinforcing liquefiable foundation
CN105714765A (en) * 2016-02-01 2016-06-29 南京林业大学 Microorganism low-temperature grouting and soil reinforcing method

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CN105507232A (en) * 2015-12-08 2016-04-20 南京林业大学 Microorganism slab grouting device and method used for reinforcing liquefiable foundation
CN105714765A (en) * 2016-02-01 2016-06-29 南京林业大学 Microorganism low-temperature grouting and soil reinforcing method

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Cited By (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108049409A (en) * 2017-11-14 2018-05-18 河海大学 A kind of method of the solid soil of microbes synthase inhibitor slip casing by pressure
CN108192623A (en) * 2018-01-10 2018-06-22 南京林业大学 Bayer process red mud roadbed microorganism curing and application process
CN108220197A (en) * 2018-01-10 2018-06-29 南京林业大学 A kind of gold tailings slag microorganism combined processing and application process
CN109165479B (en) * 2018-10-29 2020-08-04 西南大学 Method for judging soil body applicable to MICP technology
CN109165479A (en) * 2018-10-29 2019-01-08 西南大学 The soil body is applicable in the judgment method of MICP technology
CN110130318A (en) * 2019-05-24 2019-08-16 南京林业大学 A kind of construction method of microorganism mixing-filling stake
CN110284491A (en) * 2019-07-31 2019-09-27 南京林业大学 A kind of magnetized solution microorganism soil-fixing device and solid indigenous method
CN110886282A (en) * 2019-12-17 2020-03-17 西南石油大学 Particle excitation type microorganism soil stabilization wall protection device
CN110886282B (en) * 2019-12-17 2020-07-03 西南石油大学 Particle excitation type microorganism soil stabilization wall protection device
CN112030942A (en) * 2020-08-31 2020-12-04 南京林业大学 Method for improving liquefaction resistance of foundation by silicate mineral decomposing bacteria
CN114396038A (en) * 2022-01-21 2022-04-26 中煤航测遥感集团有限公司 Method for reinforcing soil strength by using microorganism induced calcium carbonate deposition
CN115247420A (en) * 2022-09-02 2022-10-28 浙江大学 Calcareous sand MICP pile based on prefabricated biological pulp and construction method thereof
CN116335115A (en) * 2023-02-21 2023-06-27 广州航海学院 Liquefied sand foundation treatment method combining high-energy gas fracturing and MICP technology
CN116397618A (en) * 2023-02-21 2023-07-07 广州航海学院 Method for reinforcing foundation of silt by combining hydraulic fracturing and MICP technology
CN116397618B (en) * 2023-02-21 2024-04-19 广州航海学院 Method for reinforcing foundation of silt by combining hydraulic fracturing and MICP technology

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