CN108220197A - A kind of gold tailings slag microorganism combined processing and application process - Google Patents
A kind of gold tailings slag microorganism combined processing and application process Download PDFInfo
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- CN108220197A CN108220197A CN201810024717.8A CN201810024717A CN108220197A CN 108220197 A CN108220197 A CN 108220197A CN 201810024717 A CN201810024717 A CN 201810024717A CN 108220197 A CN108220197 A CN 108220197A
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- bacillus
- slag
- gold tailings
- tailings
- gold
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- 239000002893 slag Substances 0.000 title claims abstract description 76
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 title claims abstract description 67
- 229910052737 gold Inorganic materials 0.000 title claims abstract description 67
- 239000010931 gold Substances 0.000 title claims abstract description 67
- 238000000034 method Methods 0.000 title claims abstract description 45
- 244000005700 microbiome Species 0.000 title claims abstract description 25
- 230000008569 process Effects 0.000 title claims abstract description 21
- 238000012545 processing Methods 0.000 title claims abstract description 21
- 241000193395 Sporosarcina pasteurii Species 0.000 claims abstract description 23
- 239000012530 fluid Substances 0.000 claims abstract description 23
- 241000194107 Bacillus megaterium Species 0.000 claims abstract description 20
- 241000194103 Bacillus pumilus Species 0.000 claims abstract description 20
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 19
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 19
- 238000007569 slipcasting Methods 0.000 claims abstract description 17
- XFXPMWWXUTWYJX-UHFFFAOYSA-N Cyanide Chemical compound N#[C-] XFXPMWWXUTWYJX-UHFFFAOYSA-N 0.000 claims abstract description 16
- 239000000243 solution Substances 0.000 claims description 42
- JMANVNJQNLATNU-UHFFFAOYSA-N oxalonitrile Chemical compound N#CC#N JMANVNJQNLATNU-UHFFFAOYSA-N 0.000 claims description 28
- 235000015097 nutrients Nutrition 0.000 claims description 25
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 20
- 239000002689 soil Substances 0.000 claims description 19
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 13
- 239000004202 carbamide Substances 0.000 claims description 13
- 241000894006 Bacteria Species 0.000 claims description 12
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 12
- 239000011148 porous material Substances 0.000 claims description 11
- AIUDWMLXCFRVDR-UHFFFAOYSA-N dimethyl 2-(3-ethyl-3-methylpentyl)propanedioate Chemical compound CCC(C)(CC)CCC(C(=O)OC)C(=O)OC AIUDWMLXCFRVDR-UHFFFAOYSA-N 0.000 claims description 10
- 239000011780 sodium chloride Substances 0.000 claims description 10
- 239000012137 tryptone Substances 0.000 claims description 8
- 239000002131 composite material Substances 0.000 claims description 6
- 238000011081 inoculation Methods 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 6
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 6
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 6
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 6
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 6
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 6
- 238000013461 design Methods 0.000 claims description 5
- 230000007613 environmental effect Effects 0.000 claims description 5
- 238000002347 injection Methods 0.000 claims description 5
- 239000007924 injection Substances 0.000 claims description 5
- 239000002002 slurry Substances 0.000 claims description 5
- 239000007787 solid Substances 0.000 claims description 5
- CAXNYFPECZCGFK-UHFFFAOYSA-N 2-phenyl-2-pyridin-2-ylacetonitrile Chemical compound C=1C=CC=NC=1C(C#N)C1=CC=CC=C1 CAXNYFPECZCGFK-UHFFFAOYSA-N 0.000 claims description 4
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 4
- 239000002054 inoculum Substances 0.000 claims description 4
- 238000000926 separation method Methods 0.000 claims description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 3
- 239000001110 calcium chloride Substances 0.000 claims description 3
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 3
- 238000000354 decomposition reaction Methods 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 229910052760 oxygen Inorganic materials 0.000 claims description 3
- 239000001301 oxygen Substances 0.000 claims description 3
- 230000007306 turnover Effects 0.000 claims description 3
- 238000006065 biodegradation reaction Methods 0.000 claims description 2
- 230000006872 improvement Effects 0.000 claims description 2
- 150000002825 nitriles Chemical group 0.000 claims description 2
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 abstract description 12
- 229910000019 calcium carbonate Inorganic materials 0.000 abstract description 6
- 239000013078 crystal Substances 0.000 abstract description 6
- 229910001385 heavy metal Inorganic materials 0.000 abstract description 5
- 230000015556 catabolic process Effects 0.000 abstract description 4
- 238000006731 degradation reaction Methods 0.000 abstract description 4
- 229910021645 metal ion Inorganic materials 0.000 abstract description 2
- 239000010970 precious metal Substances 0.000 abstract description 2
- 238000004064 recycling Methods 0.000 abstract description 2
- 238000002360 preparation method Methods 0.000 abstract 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 6
- 238000004519 manufacturing process Methods 0.000 description 6
- 239000002609 medium Substances 0.000 description 6
- 239000011707 mineral Substances 0.000 description 6
- 235000010755 mineral Nutrition 0.000 description 6
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 239000001963 growth medium Substances 0.000 description 3
- 239000002068 microbial inoculum Substances 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 239000011435 rock Substances 0.000 description 3
- 239000002910 solid waste Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 239000002699 waste material Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 239000001888 Peptone Substances 0.000 description 2
- 108010080698 Peptones Proteins 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 2
- 229910052681 coesite Inorganic materials 0.000 description 2
- 229910052593 corundum Inorganic materials 0.000 description 2
- 229910052906 cristobalite Inorganic materials 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 238000005265 energy consumption Methods 0.000 description 2
- 238000007654 immersion Methods 0.000 description 2
- 150000002500 ions Chemical class 0.000 description 2
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 235000019319 peptone Nutrition 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 239000000377 silicon dioxide Substances 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- 229910052682 stishovite Inorganic materials 0.000 description 2
- 239000002352 surface water Substances 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- 229910052905 tridymite Inorganic materials 0.000 description 2
- 229910001845 yogo sapphire Inorganic materials 0.000 description 2
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 208000035126 Facies Diseases 0.000 description 1
- 101000965313 Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513) Aconitate hydratase A Proteins 0.000 description 1
- KKCBUQHMOMHUOY-UHFFFAOYSA-N Na2O Inorganic materials [O-2].[Na+].[Na+] KKCBUQHMOMHUOY-UHFFFAOYSA-N 0.000 description 1
- 239000003570 air Substances 0.000 description 1
- 238000006701 autoxidation reaction Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 235000011148 calcium chloride Nutrition 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 238000005660 chlorination reaction Methods 0.000 description 1
- 238000005352 clarification Methods 0.000 description 1
- 239000004927 clay Substances 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000007596 consolidation process Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 238000001784 detoxification Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000011038 discontinuous diafiltration by volume reduction Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000010433 feldspar Substances 0.000 description 1
- 239000003673 groundwater Substances 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 150000007661 iron cyano complex Chemical class 0.000 description 1
- 229910052745 lead Inorganic materials 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 229910052618 mica group Inorganic materials 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 229910000510 noble metal Inorganic materials 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 210000000496 pancreas Anatomy 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 230000005641 tunneling Effects 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C04—CEMENTS; CONCRETE; ARTIFICIAL STONE; CERAMICS; REFRACTORIES
- C04B—LIME, MAGNESIA; SLAG; CEMENTS; COMPOSITIONS THEREOF, e.g. MORTARS, CONCRETE OR LIKE BUILDING MATERIALS; ARTIFICIAL STONE; CERAMICS; REFRACTORIES; TREATMENT OF NATURAL STONE
- C04B5/00—Treatment of metallurgical slag ; Artificial stone from molten metallurgical slag
Abstract
The invention discloses a kind of gold tailings slag microorganism combined processing and application processes, belong to field of road.One or more of Bacillus pasteurii (Bacillus pasteurii) and bacillus subtilis (Bacillus subtilis), bacillus pumilus (Bacillus pumilus) or bacillus megaterium (Bacillus megaterium) are used cooperatively by this method.Generation calcium carbonate crystal is deposited in the hole of gold tailings slag using Bacillus pasteurii, improves its mechanical property and stability, fixes heavy metal and precious metal ion in mine tailings.The cyanide in gold tailings slag is decomposed using bacillus subtilis, bacillus pumilus or bacillus megaterium.The method that the present invention utilizes " slip casting is turned over+taken turns more to machinery ", is fully mixed microorganism, gold tailings slag, consolidating fluid three, and mine tailings and degradation cyanide are reinforced to achieve the purpose that generate calcium carbonate crystal.This method have non-secondary pollution, it is of low cost, preparation method is simple and resource utilization is high the characteristics of, it can be achieved that mine tailings by all kinds of means, extensive recycling.
Description
Technical field
The present invention relates to a kind of gold tailings slag microorganism combined processing and application processes, belong to field of road.
Background technology
During exploitation of gold deposit, the uncommercial adjacent rock of mineral deposit and rock removing and generated when tunneling are known as barren rock.
The ore waste residue discharged after concentrate that extracts gold from is gold tailings.Golden production is generally using cyanidation gold-extracted method, in this work
Cyanide is must be added to during skill, tailing is caused also to remain a certain amount of cyanogen.Since gold tailings are mostly using at bin system
Reason, with the expansion of golden production scale and the extension of production history, gold tailings accumulating amount increases year by year, does not only take up a large amount of
Soil, pollution air, surface water and groundwater；And many Tailings Dams exceed the time limit or excess load uses or even violation operation, make tail
Ore deposit inventory is in very big security risk.At present, the comprehensive utilization ratio of China's metal tailings is average less than 10%, lags behind other significantly
Bulk solid waste.From environmental protection angle, it is necessary to these gold tailings be handled, avoid secondary pollution；From
Protection of resources angle, gold tailings, which are used, to turn waste into wealth, and turn harm into good, and alleviate China's economic development and ecological ring
Border is destroyed, the conspicuous contradiction of shortage of resources.Improve the comprehensive utilization degree of mineral resources.
Microorganism soil-fixing technology is a kind of ecology, environmentally friendly solid indigenous method.The solid soil of microorganism slip casting mainly passes through alternating
Inject microbial inoculum and consolidating fluid, using microorganism induction calcium carbonate crystal technology (MICP technologies), the hole between soil particle
Middle precipitation calcium carbonate crystal, it is cementing with filling soil particle and then playing the role of soil mass consolidation.At present mainly using micro- in engineering
Biological slip casting curing sand and silt.
Gold tailings belong to solid waste, pH ＞ 10, in alkalinity.SiO in tailing2, CaO content it is higher, contain one simultaneously
Quantitative Fe2O3、Al2O3, MgO and a small amount of noble metal (such as Au, Ag), heavy metal (such as Cu, Pb, Zn).Mineral facies are usually with stone
Based on English, feldspar, mica group, clay class and kish mineral etc.；Mineral granularity is very thin, and argillization phenomenon is serious etc..From existing
From the point of view of the specifications such as Road Design, examination or standard requirement, the unconfined compressive strength value of gold tailings, the modulus of resilience, California are held
Related request cannot be met than indexs such as (CBR) by carrying.Therefore, gold tailings cannot be directly as roadbed embankment material for public affairs
Road and town road roadbed use.
The technical solution of the prior art one
Tailings Dam is stored up：For using the tailing after all sliming cyanidation-carbon slurry therapy extracting gold, at traditional alkaline chlorination process
Cyanide heel row is managed to store up to Tailings Dam or to dry piling after tailings characteristic.
The shortcomings that prior art one
Stockpiling processing is carried out to tailing, a large amount of soil can be occupied, damages a large amount of agricultural and woods soil.
The technical solution of the prior art two
Burning method：Burning method is the integrated disposal processing of solid waste pyrolytic and deep oxidation.It can be with by burning
Oxidation operation therein is decomposed, reaches volume reduction, detoxification, recover energy and the purpose of byproduct.
The shortcomings that prior art two
Such method investment is big, high energy consumption, of high cost, recycling low production efficiency, this high investment, the life of low output
Production method is uneconomic.
The technical solution of the prior art three
Natural degradation method：Natural degradation method is to remove cyanide with natural way, is exposed by autoxidation, volatilization, sunlight
Abiogenous physics, the chemical actions such as decomposition are shone, dissociates cyanide, heavy metal ion precipitation.
The shortcomings that prior art three
Natural degradation method needs enough clarification times and oxygen exchange area and good underground anti-seepage layer etc., has and throws
The advantages such as money is less, producing cost is low.But the method, which exists, to be taken up a large area, and process is slow, is influenced greatly by natural cause, discharge waste
The shortcomings of hardly possible is up to standard, and especially iron cyano complex difficulty is proved effective.
Invention content
Technical problem：Existing gold tailings Slag treatment technology, there are environmental pollution is serious, input height, low production efficiency,
Investment is big, high energy consumption, of high cost, can not effective technical problems such as commercial Application, it is difficult to accomplish large batch of processing on a large scale, phase
It is little compared with the generation speed effect of gold tailings slag.The present invention utilizes energy-saving and environmental protection, free of contamination microorganism complex technique,
Strengthen its mechanical property again while carrying out environmental treatment to red mud, reach the requirement that ground in civil engineering uses, be
Mine tailings are extensive, industrialization administers and provides new method.
Technical solution
The principle of the present invention is：Bacillus pasteurii (the Bacillus that the present invention will belong to bacillus
Pasteurii) with bacillus subtilis (Bacillus subtilis), bacillus pumilus (Bacillus pumilus) or
One or more of bacillus megaterium (Bacillus megaterium) is used cooperatively.Utilize Bacillus pasteurii
(S.pasteurii) the deposition generation calcium carbonate crystal in the hole of gold tailings slag, cementing filling tailing solid impurity particle, not only can
Significantly improve its mechanical property and stability, moreover it is possible to heavy metal and precious metal ion in fixed mine tailings.And utilize withered grass bud
Spore bacillus, bacillus pumilus or bacillus megaterium decompose the cyanide in gold tailings slag, remove it to air, surface water
With the pollution of underground water.Very thin for gold tailings slag mineral granularity, the characteristics of argillization phenomenon is serious, the present invention utilizes " machinery
Turn over+slip casting " method, bacterium solution is sprayed when turning over first by microbial inoculum and the full and uniform mixing of mine tailings, Ran Houduo
The method of wheel slip casting provides nutriment and consolidating fluid for the microorganism being distributed in mine tailings, to reach generation calcium carbonate crystal
Reinforce the purpose of mine tailings and cyanide of degrading.
A kind of gold tailings slag microorganism combined processing and application process, it is characterised in that this method step is as follows：
(1) comminution：The mine tailings of caking are crushed and ground；
(2) strain acquisition culture：Acquisition drop cyanogen strain is put into liquid from the topsoil near Tailings Dam cyanogen-containing tailing slag
In body culture medium, and inoculation Bacillus pasteurii in it, pass through separation and the domestication culture of different gradient culture solutions containing cyanogen of crossing
Afterwards, the expansion culture step by step of strain is carried out, the composite flora after being tamed, flora is by Bacillus pasteurii (Bacillus
Pasteurii) with bacillus subtilis (Bacillus subtilis), bacillus pumilus (Bacillus pumilus) or
One or more of bacillus megaterium (Bacillus megaterium) is composed.It is included in 1L fluid nutrient mediums
The soy peptone of 5.0g, the tryptone of 15.0g, the sodium chloride of 5.0g and 10g urea, medium pH control 7.3,
The flora for completing domestication together with fluid nutrient medium with sterile soyabean protein powder by 1: 1 mass volume ratio is mixed, is stood for 24 hours
Afterwards, bacterium solution 3 is made 1..
(3) ground is laid with：Gold tailings thickness of slag layer is uniformly laid in 20cm~50cm or so；
(4) inoculation is turned over：Machinery is carried out to gold tailings slag blanket to turn over, by the 1. uniform spray of bacterium solution 3 while turning over
It is spread across in slag layer, inoculum concentration is 15~30L/t mixtures, is sufficiently mixed uniformly；
(5) (3), (4) operation are repeated, is laid with multilayer slag layer, until reaching the ground layer thickness of design；
(6) place is flattened, grouting tube 1 is vertically squeezed into gold tailings slag blanket 7；Slip casting is arranged on the place of leveling
2. the nutrient solution 3 of 1.5 times of pore volume to be consolidated by grouting tube 1, is injected, often in hole into gold tailings slag blanket 7 to be consolidated first
2. the nutrient solution 3 of 1L includes：The soy peptone of 5.0g, the tryptone of 20g, the sodium chloride of 6.0g and 15g urea, 2g
Magnesium sulfate, 5g potassium dihydrogen phosphate；Reinject the urine with the 2. isometric a concentration of 0.5mol/L~1.0mol/L of nutrient solution 3
Element and CaCl2The consolidating fluid 4 mixed；
(7) using air compressor 6, into gold category slag layer 8 to be consolidated, injected slurry volume is 8 pore volume 1.5 of the soil body to be consolidated
Compressed air again stands 12h；
(8) consolidating fluid 4 of 1.5 times of 8 pore volume of the soil body to be consolidated is injected into slag layer 8 to be consolidated, stands 12h；
(9) (6), (7), (8) operation is repeated, until Dai Gu slag layers Final 8 degree reaches requirement or slip casting liquid (bacterium solution
3 1. or consolidating fluid 4) can not inject when in solid slag layer 8, stop slip casting；After the completion of slip casting, all slip castings on place are pulled out
Pipe.
The gold tailings slag microorganism combined processing and application process, it is characterised in that composite flora used is by Pasteur
Bacillus (Bacillus pasteurii) and bacillus subtilis (Bacillus subtilis), bacillus pumilus
One or more of (Bacillus pumilus) or bacillus megaterium (Bacillus megaterium) are composed.
The gold tailings slag microorganism combined processing and application process, it is characterised in that the nutrient solution 3 in step (6)
2. comprising：The soy peptone of 5.0g, the tryptone of 20g, the sodium chloride of 6.0g and the urea of 15g, the magnesium sulfate of 2g, 5g
Potassium dihydrogen phosphate.
The gold tailings slag microorganism combined processing and application process, it is characterised in that using injection in step (7)
The method of compressed air provides required oxygen for the decomposition of cyanide, accelerates the biodegradation rate of cyanide.
The gold tailings slag microorganism combined processing and application process, it is characterised in that after biodegradable curing process
Its mechanical property of mine tailings and environmental index meet the requirement of engineer application, available for roadbed filling engineering, realize tail
The extensive improvement of slag.
Beneficial effects of the present invention：
(1) Bacillus pasteurii (Bacillus pasteurii) and bacillus subtilis (Bacillus subtilis),
One kind or several in bacillus pumilus (Bacillus pumilus) or bacillus megaterium (Bacillus megaterium)
Kind is applied in combination, and can not only decompose the cyanide in gold tailings slag, but also can play curing gold tailings slag and be used as building work
The effect of journey filling material can quickly handle a large amount of gold tailings slags, the new way that one gold tailings Slag treatment of developing utilizes.
(2) method that filling slurry is turned over using machinery, can realize the full and uniform mixing of microbial inoculum and mine tailings；
To nutrient solution and consolidating fluid using the method for more wheel slip castings, sufficient nutrition can be provided for the microorganism being distributed in mine tailings
Substance and consolidating fluid make cyanide adequately be degraded and the gold tailings slag after curing are made to reach very high intensity.
(3) performances such as gold tailings slag its mechanical characteristic after microorganism combined processing, water stability have significantly
It improves, the requirement that engineering uses can be met.Research shows that after microbiological treatment, non-immersions in 7 days of gold tailings slag are anti-
Compressive Strength is promoted to 1.6MPa, and compressive strength after immersion is promoted to 1.54MPa within 7 days.
(4) the gold tailings slag after microorganism combined processing, wherein the toxic cyanide contained is decomposed, (residue contains
Amount less than initial content 1%), heavy metal ion fixed, avoid the pollution to environment such as underground water, rainwater.
(5) entire gold tailings Slag treatment process carries out under room temperature, condition of normal pressure, is accumulated with no cyanogen root and without secondary
The advantages of pollution, can effectively remove cyanide, easy to operate, have the advantages such as good biocompatibility.
Description of the drawings
Fig. 1 slip casting schematic diagrames
Reference numeral：1- grouting tubes, 2- grouting pumps, 3 1.-bacterium solution, 3 2.-nutrient solution, 4- consolidating fluids, 5- valves, 6- are empty
Air compressor, 7- mine tailings grounds, 8- mine tailings to be consolidated
Specific embodiment
Gold tailings slag source is Shandong Gold Group, main component SiO2.Wherein, pH ＞ 10.0, chemical composition such as the following table 1
It is shown：
Table 1
| Component | SiO2 | Al2O3 | Fe2O3 | MgO | CaO | Na2O |
| Content/% | 65~85 | 5~15 | 1~4 | 0.5~8 | 0.5~2 | 0.5~4 |
Embodiment 1
In this example, gold tailings slag microorganism combined processing and application process are used to handle mine tailings ground, this method
Include the following steps：
(1) comminution：The mine tailings of caking are crushed and ground；
(2) strain acquisition culture：Acquisition drop cyanogen strain is put into liquid from the topsoil near Tailings Dam cyanogen-containing tailing slag
In body culture medium, and inoculation Bacillus pasteurii in it, pass through separation and the domestication culture of different gradient culture solutions containing cyanogen of crossing
Afterwards, the expansion culture step by step of strain is carried out, the composite flora after being tamed, flora is by Bacillus pasteurii (Bacillus
Pasteurii) with bacillus subtilis (Bacillus subtilis), bacillus pumilus (Bacillus pumilus) or
One or more of bacillus megaterium (Bacillus megaterium) is composed.It is included in 1L fluid nutrient mediums
The soy peptone of 5.0g, the tryptone of 15.0g, the sodium chloride of 5.0g and 10g urea, medium pH control 7.3,
The flora for completing domestication together with fluid nutrient medium with sterile soyabean protein powder by 1: 1 mass volume ratio is mixed, is stood for 24 hours
Afterwards, bacterium solution 3 is made 1.；
(3) ground is laid with：Gold tailings thickness of slag layer is uniformly laid in 30cm or so；Pending slag is turned over using machinery
1. layer, ploughed depth 30cm apply bacterium solution 3, inoculum concentration 15L/t, with gold during plowed soils to mine tailings
Mine tailings are thoroughly mixed uniformly；
(4) (3) operation is repeated, is laid with multilayer slag layer, until reaching the ground layer thickness 1.5m of design；
(5) place is flattened, grouting tube 1 is vertically squeezed into gold tailings slag blanket 7；
(6) it arranges injected hole on the place of leveling, by grouting tube 1, is noted first into gold tailings slag blanket 7 to be consolidated
Enter the nutrient solution 3 of 1.5 times of pore volume to be consolidated 2., the nutrient solution 2 per 1L includes：The soy peptone of 5.0g, the tryptose of 20g
Peptone, the urea of the sodium chloride of 6.0g and 15g, the magnesium sulfate of 2g, 5g potassium dihydrogen phosphate；It reinjects and 2. waits bodies with nutrient solution 3
The urea and CaCl of long-pending a concentration of 0.75mol/L2The consolidating fluid 4 mixed；
(7) pressure of 1.5 times of 8 pore volume of the soil body to be consolidated is injected into gold category slag layer 8 to be consolidated using air compressor 6
Contracting air stands 12h；
(8) consolidating fluid 4 of 1.5 times of its pore volume is injected into the soil body 8 to be consolidated using Grouting Pipe, stands 12h；
(9) using Grouting Pipe into pending stratum alternately injection nutrient solution 3 2., air and cementing solution 4, alternately note
It is 6 times to starch number；
(10) after the completion of slip casting, all Grouting Pipes on place are pulled out.
Using and effect：The toxicity of foundation bearing capacity and soil is satisfied by requiring, and unconfined compressive strength increases to
1.1MPa, pH value are reduced to 7.8；Contained cyanide drops to 4.11mg/kg after 30 days by initial 423mg/kg in slag.
Embodiment 2
In this example, gold tailings slag microorganism combined processing and application process are used to handle mine tailings ground, this method
Include the following steps：
(1) comminution：The mine tailings of caking are crushed and ground；
(2) strain acquisition culture：Acquisition drop cyanogen strain is put into liquid from the topsoil near Tailings Dam cyanogen-containing tailing slag
In body culture medium, and inoculation Bacillus pasteurii in it, pass through separation and the domestication culture of different gradient culture solutions containing cyanogen of crossing
Afterwards, the expansion culture step by step of strain is carried out, the composite flora after being tamed, flora is by Bacillus pasteurii (Bacillus
Pasteurii) with bacillus subtilis (Bacillus subtilis), bacillus pumilus (Bacillus pumilus) or
One or more of bacillus megaterium (Bacillus megaterium) is composed.It is included in 1L fluid nutrient mediums
The soy peptone of 5.0g, the tryptone of 15.0g, the sodium chloride of 5.0g and 10g urea, medium pH control 7.3,
The flora for completing domestication together with fluid nutrient medium with sterile soyabean protein powder by 1: 1 mass volume ratio is mixed, is stood for 24 hours
Afterwards, bacterium solution 3 is made 1.；
(3) ground is laid with：Gold tailings thickness of slag layer is uniformly laid in 50cm or so；Pending slag is turned over using machinery
1. layer, ploughed depth 50cm apply bacterium solution 3 during plowed soils to soil, the inoculum concentration of bacterium solution 3 1. is 30L/t,
It is thoroughly mixed uniformly with gold tailings slag；
(4) (3) operation is repeated, is laid with multilayer slag layer, until reaching the ground layer thickness 2.5m of design；
(5) place is flattened, grouting tube 1 is vertically squeezed into gold tailings slag blanket 7；
(6) it arranges injected hole on the place of leveling, by grouting tube 1, is noted first into gold tailings slag blanket 7 to be consolidated
Enter the nutrient solution 3 of 2.5 times of pore volume to be consolidated 2., 2. the nutrient solution 3 per 1L includes：The soy peptone of 5.0g, the pancreas egg of 20g
White peptone, the urea of the sodium chloride of 6.0g and 15g, the magnesium sulfate of 2g, 5g potassium dihydrogen phosphate；2. etc. it reinjects with nutrient solution 3
The consolidating fluid 4 that the urea of a concentration of 0.5mol/L of volume is mixed with CaCl2；
(7) using air compressor 6, into gold tailings slag blanket 8 to be consolidated, injected slurry volume is 8 pore volume 1.5 of the soil body to be consolidated
Compressed air again is stood for 24 hours；
(8) it is stood for 24 hours using Grouting Pipe toward the cementing solution 4 of gold tailings slag blanket injection to be consolidated is waited；
(9) using Grouting Pipe into pending stratum alternately injection nutrient solution 3 2., air and cementing solution 4, alternately note
It is 8 times to starch number；
(10) after the completion of slip casting, all Grouting Pipes on place are pulled out.
Using and effect：The toxicity of foundation bearing capacity and soil is satisfied by requiring, and unconfined compressive strength increases to
1.6MPa, pH value are reduced to 7.4；Contained cyanide drops to 4.84mg/kg after 30 days by initial 423mg/kg in slag.
Claims (5)
- Gold tailings slag microorganism combined processing and application process described in 1., it is characterised in that composite flora used is Pasteur's bud Spore bacillus (Bacillus pasteurii) and bacillus subtilis (Bacillus subtilis), bacillus pumilus One or more of (Bacillus pumilus) or bacillus megaterium (Bacillus megaterium) are applied in combination.
- Gold tailings slag microorganism combined processing and application process described in 2., it is characterised in that this method step is as follows：(1) comminution：The mine tailings of caking are crushed and ground；(2) strain acquisition culture：Acquisition drop cyanogen strain is put into liquid training from the topsoil near Tailings Dam cyanogen-containing tailing slag It supports in base, and inoculation Bacillus pasteurii in it, after cross separation and the domestication culture of different gradient culture solutions containing cyanogen, into The expansion culture step by step of row strain, the composite flora after being tamed, flora is by Bacillus pasteurii (Bacillus Pasteurii) with bacillus subtilis (Bacillus subtilis), bacillus pumilus (Bacillus pumilus) or One or more of bacillus megaterium (Bacillus megaterium) is composed.It is included in 1L fluid nutrient mediums The soy peptone of 5.0g, the tryptone of 15.0g, the sodium chloride of 5.0g and 10g urea, medium pH control 7.3, The flora for completing domestication together with fluid nutrient medium with sterile soyabean protein powder by 1: 1 mass volume ratio is mixed, is stood for 24 hours Afterwards, bacterium solution 3 is made 1..(3) ground is laid with：Gold tailings thickness of slag layer is uniformly laid in 20cm~50cm or so；(4) inoculation is turned over：Machinery is carried out to gold tailings slag blanket to turn over, and is 1. uniformly sprayed onto bacterium solution 3 while turning over In slag layer, inoculum concentration is 15~30L/t mixtures, is sufficiently mixed uniformly；(5) (3), (4) operation are repeated, is laid with multilayer slag layer, until reaching the ground layer thickness of design；(6) place is flattened, grouting tube 1 is vertically squeezed into gold tailings slag blanket 7；Injected hole is arranged on the place of leveling, By grouting tube 1, the nutrient solution 3 of 1.5 times of pore volume to be consolidated is injected into gold tailings slag blanket 7 to be consolidated first 2., per 1L Nutrient solution 3 2. include：The soy peptone of 5.0g, the tryptone of 20g, the urea of the sodium chloride of 6.0g and 15g, 2g The potassium dihydrogen phosphate of magnesium sulfate, 5g；Reinject the urea with the 2. isometric a concentration of 0.5mol/L~1.0mol/L of nutrient solution 3 With CaCl2The consolidating fluid 4 mixed；(7) using air compressor 6, into gold tailings slag blanket 8 to be consolidated, injected slurry volume is 1.5 times of 8 pore volume of the soil body to be consolidated Compressed air stands 12h；(8) consolidating fluid 4 of 1.5 times of 8 pore volume of the soil body to be consolidated is injected into slag layer 8 to be consolidated, stands 12h；(9) (6), (7), (8) operation is repeated, (bacterium solution 3 is 1. until Dai Gu slag layers Final 8 degree reaches requirement or slip casting liquid Or consolidating fluid 4) can not inject when in solid slag layer 8, stop slip casting；After the completion of slip casting, all Grouting Pipes on place are pulled out.
- Gold tailings slag microorganism combined processing and application process described in 3., it is characterised in that the nutrient solution 3 in step (6) is 2. Comprising：The soy peptone of 5.0g, the tryptone of 20g, the sodium chloride of 6.0g and the urea of 15g, the magnesium sulfate of 2g, 5g Potassium dihydrogen phosphate.
- Gold tailings slag microorganism combined processing and application process described in 4., it is characterised in that using injection pressure in step (7) The method of contracting air provides required oxygen for the decomposition of cyanide, accelerates the biodegradation rate of cyanide.
- Gold tailings slag microorganism combined processing and application process described in 5., it is characterised in that after biodegradable curing process Its mechanical property of mine tailings and environmental index meet the requirement of engineer application, available for roadbed filling engineering, realize tailing The extensive improvement of slag.
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| CN110156429A (en) * | 2019-05-21 | 2019-08-23 | 重庆大学 | A kind of manganese ore tailing waste residue brick-making method and product based on microorganism |
| CN110204289A (en) * | 2019-06-19 | 2019-09-06 | 重庆大学 | Solidify the method and products thereof of CHARACTERISTICS OF TAILINGS SAND based on activated magnesia microorganism |
| CN111672900A (en) * | 2020-06-01 | 2020-09-18 | 合肥工业大学 | Method for restoring heavy metal pollution of soil by microorganism-induced calcium carbonate precipitation |
| CN112779182A (en) * | 2020-12-31 | 2021-05-11 | 武汉工程大学 | Portable microorganism-induced calcium carbonate precipitation kit and application thereof in repairing early cracks of pavement base |
| CN113245348A (en) * | 2021-05-14 | 2021-08-13 | 浙江工业大学 | Method for solidifying heavy metal in tailings by using halophilic bacillus |
| CN113399451A (en) * | 2021-07-26 | 2021-09-17 | 辽宁工程技术大学 | Method for solidifying and repairing lead-zinc tailings |
| CN114804732A (en) * | 2022-03-21 | 2022-07-29 | 合肥工业大学 | Method for preparing roadbed filler by using alkaline residue and microorganism modified tailing sand |
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| CN111672900A (en) * | 2020-06-01 | 2020-09-18 | 合肥工业大学 | Method for restoring heavy metal pollution of soil by microorganism-induced calcium carbonate precipitation |
| CN111672900B (en) * | 2020-06-01 | 2021-08-10 | 合肥工业大学 | Method for restoring heavy metal pollution of soil by microorganism-induced calcium carbonate precipitation |
| CN112779182A (en) * | 2020-12-31 | 2021-05-11 | 武汉工程大学 | Portable microorganism-induced calcium carbonate precipitation kit and application thereof in repairing early cracks of pavement base |
| CN113245348A (en) * | 2021-05-14 | 2021-08-13 | 浙江工业大学 | Method for solidifying heavy metal in tailings by using halophilic bacillus |
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