CN110144876B - Microorganism soil fixation method for controlling cementation rate - Google Patents
Microorganism soil fixation method for controlling cementation rate Download PDFInfo
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- CN110144876B CN110144876B CN201910454060.3A CN201910454060A CN110144876B CN 110144876 B CN110144876 B CN 110144876B CN 201910454060 A CN201910454060 A CN 201910454060A CN 110144876 B CN110144876 B CN 110144876B
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- E—FIXED CONSTRUCTIONS
- E02—HYDRAULIC ENGINEERING; FOUNDATIONS; SOIL SHIFTING
- E02D—FOUNDATIONS; EXCAVATIONS; EMBANKMENTS; UNDERGROUND OR UNDERWATER STRUCTURES
- E02D3/00—Improving or preserving soil or rock, e.g. preserving permafrost soil
- E02D3/12—Consolidating by placing solidifying or pore-filling substances in the soil
Abstract
A microorganism soil fixation method for controlling cementation rate solves the problem of controllability of microorganism soil fixation cementation rate, enables the microorganism soil fixation rate to be controllable, and improves the success rate of soil fixation by a microorganism stirring method, and is characterized by comprising the following steps: a. weighingAdding 2-10 parts of alkaline lignin into 40 parts of distilled water, heating at 35 ℃ and stirring for 20min, adding 1-5 parts of sodium-based montmorillonite powder, continuing stirring for 0.5h, adding 10 parts of urea, heating at 42 ℃ and stirring for 0.5h, and then putting into an oven to be dried to obtain a nitrogen source urea mixture; b. grinding the dried nitrogen source urea mixture to a particle size of less than 1 mm; c. 1 part of ground nitrogen source urea mixture, 1 part of CaSO4And mixing the powder with 6-10 parts of soil to be solidified, adding 3 parts of calcium salt bacteria liquid, stirring for 1-3 hours, and standing for 12-24 hours to finish soil solidification.
Description
Technical Field
The invention relates to a microbial soil fixation method for improving the working performance of microbial soil fixation, and belongs to the technical field of foundation treatment.
Background
The microorganism soil-fixing technology is an environment-friendly and green soil-fixing technology, utilizes specific microorganisms to induce calcium carbonate precipitation to cement loose soil particles so as to improve the compressive strength of a soil body and reduce the compressibility, and can be used for foundation reinforcement and underground water leakage plugging. When the microorganism solidifies the soil body and consolidates the foundation, the controllability of the consolidation cementation rate greatly influences the implementation effect of the construction site. Microorganism-induced calcium carbonate reacts too fast, often causes the near jam of slip casting mouth when slip casting consolidates the soil body, consolidates the soil body inhomogeneous, and intensity is low when stirring method consolidates the soil body, can't form intensity even, and material utilization is low.
Technical scheme of prior art I
The acid-base regulation method includes mixing the mixed liquid of bacteria liquid, urea and calcium chloride to form microbial colloidal suspension, adding acid liquid to regulate pH value to make the microbial colloidal suspension acidic and inhibit calcium carbonate crystallization, injecting or stirring the microbial colloidal suspension into soil or soaking soil in the microbial colloidal suspension, hydrolyzing urea with microbe to produce great amount of ammonium ions to make the soil pore liquid alkaline after pH value is raised, and cementing the soil after calcium carbonate crystallization.
Disadvantages of the first prior art
The microbial colloidal suspension is gradually changed from acidity to alkaline environment which is beneficial to calcium carbonate deposition and crystallization by relying on the natural metabolism of microorganisms and the urea hydrolysis process, the reaction process is usually slow, the microbial colloidal suspension needs to be replaced or injected for many times, and the reaction rate controllability is poor.
Technical scheme of prior art II
The two-stage grouting method comprises the steps of firstly injecting bacterial liquid into a soil body, then injecting a mixed liquid of urea and calcium chloride into the soil body, controlling the total amount of a single-round reactant by adjusting the concentration of the mixed liquid, delaying the pore blocking time of the soil body, and reducing the nonuniformity of the solidified soil body.
The second prior art has the defects
The bacteria liquid and the mixed liquid of calcium chloride and urea can react quickly, the speed is difficult to control, the bacteria liquid can only be used for reinforcing soil bodies through multiple times of grouting, the soil fixing period is long, the waste liquid discharge is large, the environment is not protected, and the uniformity of the solidified soil body is poor.
Disclosure of Invention
The technical problem is as follows: the existing microorganism stirring technology for reinforcing the foundation has more advantages than the uniformity of soil reinforcement by a microorganism grouting method, but during stirring, bacterial liquid can quickly react after meeting with calcium chloride and urea solution, the microorganism cementing reaction rate is too high, inter-granular cementation is formed in the soil body in the stirring process, and the soil body is stirred and damaged, so that the strength of the stirred and solidified soil body is low, and even the solidification is ineffective. The invention aims to solve the problem of controllability of the microorganism soil fixation and cementation rate, control the microorganism soil fixation and cementation rate and improve the success rate of soil fixation by a microorganism stirring method.
Technical scheme
The principle of the invention is as follows: the release rate of nitrogen source urea is controlled through hydrogen bond recombination among hydroxyl groups, the total release amount is not remarkably reduced, the dissolved salt content in the reaction solution is controlled by insoluble calcium salt to maintain the high activity of microorganisms, the controllable soil consolidation and cementation rate of the microorganisms is realized, and the higher material utilization rate is ensured.
A microorganism soil fixation method for controlling cementation rate is characterized by comprising the following steps:
a. weighing 2-10 parts of alkaline lignin, adding the alkaline lignin into 40 parts of distilled water, heating and stirring at 35 ℃ for 20min, adding 1-5 parts of sodium-based montmorillonite powder, continuing stirring for 0.5h, adding 10 parts of urea, heating and stirring at 42 ℃ for 0.5h, and then putting the mixture into an oven to be dried to obtain a nitrogen source urea mixture;
b. grinding the dried nitrogen source urea mixture to a particle size of less than 1 mm;
c. 1 part of ground nitrogen source urea mixture, 1 part of CaSO4Mixing the powder with 6-10 parts of soil body to be solidified, adding 3 parts of calcium salt bacteria solution, stirring for 1-3 h, standing for 12-24 h to complete soil solidification。
The microbial soil fixation method for controlling the cementation rate is characterized in that in the step a, the cementation rate of the microbial soil fixation is controlled by adjusting the mass ratio of alkaline lignin, sodium-based montmorillonite powder and urea, wherein the colloid value of the sodium-based montmorillonite powder is 110-150 ml/15 g.
The microbial soil stabilization method for controlling the cementation rate is characterized in that in the step c, the calcium salt bacterium liquid is prepared from bacillus pasteurii, a culture solution and CaCl2Composition, bacterial liquid concentration OD600＝1.0～1.5，CaCl2The concentration is 3.5-6 mol/L.
The invention has the beneficial effects that: (1) the construction speed of the microbial mixing pile on site can be matched, the cementing time of the microbial soil fixation is controlled to change within 1-3 h, and the method is suitable for the working condition of the engineering site; (2) the problem of edge damage of the cementation side among soil particles in the soil solidification process by a microbial stirring method is solved, and the cementation efficiency of the material is obviously improved; (3) the soil-fixing effect with high strength can be obtained only by one-time stirring on the site, the process of microorganism soil-fixing is greatly simplified, the construction efficiency is improved, and the construction cost is reduced.
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Detailed Description
Example 1
In the embodiment, the microorganism soil stabilization method for controlling the cementation rate is used for reinforcing silt, and the method comprises the following steps:
a. weighing 6 parts of alkaline lignin, adding the alkaline lignin into 40 parts of distilled water, heating and stirring at 35 ℃ for 20min, adding 3 parts of sodium-based montmorillonite powder, continuously stirring for 0.5h, adding 10 parts of urea, heating and stirring at 42 ℃ for 0.5h, and then putting the mixture into a drying oven to be dried to obtain a nitrogen source urea mixture;
b. grinding the dried nitrogen source urea mixture to a particle size of less than 1 mm;
c. 1 part of ground nitrogen source urea mixture, 1 part of CaSO4And mixing the powder with 8 parts of soil to be solidified, adding 3 parts of calcium salt bacteria solution, stirring for 2 hours, and standing for 18 hours to finish soil solidification.
In the step a of the embodiment, the microbial soil fixation and cementation rate is controlled by adjusting the mass ratio of the alkaline lignin, the sodium-based montmorillonite powder and the urea, and the colloid value of the sodium-based montmorillonite powder is 110-125 ml/15 g.
In step c of this example, the calcium salt solution is prepared from Bacillus pasteurianus, culture solution and CaCl2Composition, bacterial liquid concentration OD600＝1.0～1.5，CaCl2The concentration is 3.5-6 mol/L.
Example 2
In this example, a microorganism soil fixation method for controlling cementation rate is used for reinforcing silty clay, and the method comprises the following steps:
a. weighing 2 parts of alkaline lignin, adding the alkaline lignin into 40 parts of distilled water, heating and stirring at 35 ℃ for 20min, adding 1 part of sodium-based montmorillonite powder, continuously stirring for 0.5h, adding 10 parts of urea, heating and stirring at 42 ℃ for 0.5h, and then putting the mixture into an oven to be dried to obtain a nitrogen source urea mixture;
b. grinding the dried nitrogen source urea mixture to a particle size of less than 1 mm;
c. 1 part of ground nitrogen source urea mixture, 1 part of CaSO4And mixing the powder with 10 parts of soil body to be solidified, adding 3 parts of calcium salt bacteria liquid, stirring for 3 hours, and standing for 24 hours to finish soil solidification.
In the step a of the embodiment, the microbial soil fixation and cementation rate is controlled by adjusting the mass ratio of the alkaline lignin, the sodium-based montmorillonite powder and the urea, and the colloid value of the sodium-based montmorillonite powder is 125-140 ml/15 g.
In step c of this example, the calcium salt solution is prepared from Bacillus pasteurianus, culture solution and CaCl2Composition, bacterial liquid concentration OD600＝1.0～1.5，CaCl2The concentration is 3.5-6 mol/L.
Example 3
In this example, a microorganism soil fixation method for controlling cementation rate is used for reinforcing sandy soil, and the method comprises the following steps:
a. weighing 10 parts of alkaline lignin, adding the alkaline lignin into 40 parts of distilled water, heating and stirring at 35 ℃ for 20min, adding 5 parts of sodium-based montmorillonite powder, continuously stirring for 0.5h, then adding 10 parts of urea, heating and stirring at 42 ℃ for 0.5h, and then putting the mixture into a drying oven to be dried to obtain a nitrogen source urea mixture;
b. grinding the dried nitrogen source urea mixture to a particle size of less than 1 mm;
c. 1 part of ground nitrogen source urea mixture, 1 part of CaSO4And mixing the powder with 6 parts of soil to be solidified, adding 3 parts of calcium salt bacteria solution, stirring for 1 hour, and standing for 12 hours to finish soil solidification.
In the step a of the embodiment, the microbial soil fixation and cementation rate is controlled by adjusting the mass ratio of the alkaline lignin, the sodium-based montmorillonite powder and the urea, and the colloid value of the sodium-based montmorillonite powder is 140-150 ml/15 g.
In step c of this example, the calcium salt solution is prepared from Bacillus pasteurianus, culture solution and CaCl2Composition, bacterial liquid concentration OD600＝1.0～1.5，CaCl2The concentration is 3.5-6 mol/L.
Claims (3)
1. A microorganism soil fixation method for controlling cementation rate is characterized by comprising the following steps:
a. weighing 2-10 parts of alkaline lignin, adding the alkaline lignin into 40 parts of distilled water, heating and stirring at 35 ℃ for 20min, adding 1-5 parts of sodium-based montmorillonite powder, continuing stirring for 0.5h, adding 10 parts of urea, heating and stirring at 42 ℃ for 0.5h, and then putting the mixture into an oven to be dried to obtain a nitrogen source urea mixture;
b. grinding the dried nitrogen source urea mixture to a particle size of less than 1 mm;
c. 1 part of ground nitrogen source urea mixture, 1 part of CaSO4And mixing the powder with 6-10 parts of soil to be solidified, adding 3 parts of calcium salt bacteria liquid, stirring for 1-3 hours, and standing for 12-24 hours to finish soil solidification.
2. The microbial soil stabilization method for controlling the cementation rate of claim 1, wherein the microbial soil stabilization cementation rate is controlled by adjusting the mass ratio of the alkaline lignin, the sodium-based montmorillonite powder and the urea in the step a, and the colloid value of the sodium-based montmorillonite powder is 110-150 ml/15 g.
3. Root of herbaceous plantThe method of claim 1 wherein the step c comprises the step of mixing the calcium salt solution with the culture solution of Pasteurella pasteurella and CaCl2Composition, bacterial liquid concentration OD600＝1.0～1.5，CaCl2The concentration is 3.5-6 mol/L.
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| CN110886282B (en) * | 2019-12-17 | 2020-07-03 | 西南石油大学 | Particle excitation type microorganism soil stabilization wall protection device |
| CN111411127B (en) * | 2020-03-30 | 2021-05-07 | 中国地质大学（北京） | Method for inducing calcium carbonate precipitation by using microorganisms |
| CN112122331B (en) * | 2020-08-31 | 2022-07-26 | 南京林业大学 | Method for solidifying and stabilizing heavy metal polluted soil |
| CN114396038A (en) * | 2022-01-21 | 2022-04-26 | 中煤航测遥感集团有限公司 | Method for reinforcing soil strength by using microorganism induced calcium carbonate deposition |
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