CN108008110A - A kind of microorganism based on temperature conditionss cures sand optimization method - Google Patents
A kind of microorganism based on temperature conditionss cures sand optimization method Download PDFInfo
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- CN108008110A CN108008110A CN201711013226.5A CN201711013226A CN108008110A CN 108008110 A CN108008110 A CN 108008110A CN 201711013226 A CN201711013226 A CN 201711013226A CN 108008110 A CN108008110 A CN 108008110A
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- Prior art keywords
- microorganism
- sand
- cures
- optimization method
- bacillus pasteurii
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/24—Earth materials
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/40—Soil-conditioning materials or soil-stabilising materials containing mixtures of inorganic and organic compounds
- C09K17/48—Organic compounds mixed with inorganic active ingredients, e.g. polymerisation catalysts
- C09K17/50—Organic compounds mixed with inorganic active ingredients, e.g. polymerisation catalysts the organic compound being of natural origin, e.g. cellulose derivatives
-
- E—FIXED CONSTRUCTIONS
- E02—HYDRAULIC ENGINEERING; FOUNDATIONS; SOIL SHIFTING
- E02D—FOUNDATIONS; EXCAVATIONS; EMBANKMENTS; UNDERGROUND OR UNDERWATER STRUCTURES
- E02D3/00—Improving or preserving soil or rock, e.g. preserving permafrost soil
- E02D3/12—Consolidating by placing solidifying or pore-filling substances in the soil
Abstract
Invention provides a kind of microorganism based on temperature conditionss and cures sand optimization method.This method includes preparing Bacillus pasteurii microorganism suspension, configures reaction solution, prepares mixed liquor, pouring into target sand sample.It this method reduce CaCO3The dispersion degree of generation, improves the validity of MICP technologies.The reaction rate and cementing strength of microorganism soil-fixing material are controllable, reduce processing cost, short construction period.
Description
Technical field
The present invention relates to Geotechnical Engineering field, and in particular to it is excellent that a kind of microorganism based on temperature conditionss cures sand
Change method.
Background technology
Microorganism adds the solidified earth research field newer as environment in recent years ground, its core technology is MICP
(Microbially Induced Calcite Precipitation, microorganism induction precipitation of calcium carbonate), is made by microorganism
With, by it is a kind of it is environmentally protective in a manner of, decomposing urea, precipitation of calcium carbonate is formed between soil body particle, is consolidated so as to reach the soil body
Change, improve soil body rigidity and intensity.
However, being repeatedly passed through method using multiphase in tradition MICP technologies, " first contacting, first react " causes easily blocking, pole
Non-uniform problem.Studying data at home and abroad up to now, it is difficult to the homogeneity question and MICP technical efficiency problems of control,
It is the principal element that restriction micro-organisms add solidified earth to apply.
The content of the invention
The object of the present invention is to provide a kind of microorganism based on temperature conditionss to cure sand optimization method, existing to solve
Problem present in technology.
To realize that the technical solution that the object of the invention uses is such, a kind of microorganism based on temperature conditionss cures
Sand optimization method, comprises the following steps：
1) Bacillus pasteurii microorganism suspension is prepared.Wherein, the Bacillus pasteurii microorganism suspension 600nm
OD value OD600=1.628~1.821.The storage temperature of the Bacillus pasteurii microorganism suspension is 4 DEG C.
2) 10 DEG C of calcium chloride solutions and 10 DEG C of urea liquid mixed configuration reaction solutions are used, and it is 10 DEG C to keep temperature.Its
In, the concentration of the calcium chloride solution is 0.25~2mol/L, and the concentration of urea liquid is 0.25~2mol/L.The calcium chloride
The volume ratio of solution and urea liquid is 1:1, concentration ratio 1:1.
3) 10 DEG C of reaction solutions and 4 DEG C of Bacillus pasteurii microorganism suspensions are taken to prepare to obtain mixed liquor.Wherein, it is described anti-
The volume ratio for answering liquid and Bacillus pasteurii microorganism suspension is 20:1~10:1.
4) peristaltic pump is opened, is poured into low-temperature mixed liquid in target sand sample under assigned rate by conduit.Make mixed liquor
Spread, and be adsorbed on soil body particle into sand sample.
5) after pouring into, sand sample 10h is stood.
6) peristaltic pump is opened, the mixed liquor extraction in sand sample is injected by liquid storing barrel by conduit.
7) repeat step 4)~6), until sand sample meets to reinforce target strength.
Further, the internal diameter of the conduit is 3.1mm.
Further, in step 1), the Bacillus pasteurii microorganism suspension 600nm OD values OD600=1.75.
Further, in step 2), the concentration of the calcium chloride solution is 2mol/L, and the concentration of urea liquid is 2mol/L.
Further, in step 4), the volume ratio of reaction solution and Bacillus pasteurii microorganism suspension is 15:1.
Further, in step 4), the irrigation rate of mixed liquor is 0.5~1mm/min.
Further, in step 4), the infusion time of mixed liquor is controlled within 1h.
The solution have the advantages that unquestionable：
A. the dispersion degree of calcium carbonate generation is reduced, improves the validity of MICP technologies；
B. the reaction rate of microorganism soil-fixing material and cementing strength controllability improve, short construction period；
C. realize that bacterial suspension is mixed with the direct of reaction solution, improve stock utilization, save processing cost.
Brief description of the drawings
Fig. 1 reinforces Optimum Experiment schematic diagram for sand column small sample；
Fig. 2 consolidates sand test schematic diagram for desert surface.
In figure：Mixed liquor 1, peristaltic pump 2, conduit 3, sand sample 4.
Embodiment
With reference to embodiment, the invention will be further described, but should not be construed the above-mentioned subject area of the present invention only
It is limited to following embodiments.Without departing from the idea case in the present invention described above, according to ordinary skill knowledge and used
With means, various replacements and change are made, should all be included within the scope of the present invention.
Embodiment 1：
Inventor's research observes that microorganism has the feature that decomposing urea activity is low under cryogenic.For existing skill
Problem present in art, inventor propose the single-phase MICP methods of low temperature using this property.This method core is, in low temperature bar
Under part, microbial activity is relatively low, directly can mix bacterium with reaction solution, low temperature is passed through soil body sample, is uniformly dispersed as far as possible.
With temperature recovery, microbial activity improves, and decomposing urea activity enhancing, calcium carbonate largely generates.
The present embodiment discloses a kind of microorganism for being applied to sand column small sample reinforcing Optimum Experiment based on temperature conditionss and consolidates
Change sand optimization method, comprise the following steps：
1) normal sand that quality is 140g~160g is weighed, sand sample 4 is prepared using sand rain method.Wherein, the sand sample 4 is overall
For a cylinder, a height of 80mm of this cylinder, a diameter of 39.1mm.
2) Bacillus pasteurii microorganism suspension is prepared.Wherein, the Bacillus pasteurii microorganism suspension 600nm
OD value OD600=1.75.The storage temperature of the Bacillus pasteurii microorganism suspension is 4 DEG C.
3) 10 DEG C of calcium chloride solutions and 10 DEG C of urea liquid mixed configuration reaction solutions are used, and it is 10 DEG C to keep temperature.Its
In, the concentration of the calcium chloride solution is 2mol/L, and the concentration of urea liquid is 2mol/L.The calcium chloride solution and urea are molten
The volume ratio of liquid is 1:1, concentration ratio 1:1.
4) 10 DEG C of reaction solutions and 4 DEG C of Bacillus pasteurii microorganism suspensions are taken to prepare to obtain mixed liquor 1.Wherein, it is described
The volume ratio of reaction solution and Bacillus pasteurii microorganism suspension is 15:1.
5) referring to Fig. 1, peristaltic pump 2 is opened, by conduit 3 by low-temperature mixed liquid 1 in 0.5~1mm/min (about 5~10ml/
Min) poured under speed in target sand sample 4.Mixed liquor 1 is spread into sand sample 4, and be adsorbed on soil body particle.Wherein, institute
The internal diameter for stating conduit 3 is 3.1mm.The infusion time of the mixed liquor 1 is controlled within 1h.
6) after 4 temperature recovery stabilization of sand sample 10h is stood to after 26 °.
7) peristaltic pump 2 is opened, injection liquid storing barrel out is extracted the mixed liquor 1 in sand sample 4 by conduit 3.
8) repeat step 5)~7), until sand sample 4 meets to reinforce target strength.
The present embodiment can be avoided caused by " first contact, first react " easily compared with conventionally employed multiphase is repeatedly passed through method
Block and extremely non-uniform problem.
Embodiment 2：
The present embodiment discloses a kind of microorganism curing sand for being applied to desert surface and consolidating sand test based on temperature conditionss
Optimization method, comprises the following steps：
1) cleaning and smooth target desert place；According to site topography, area, design and arrange conduit system；
2) Bacillus pasteurii microorganism suspension is prepared.Wherein, the Bacillus pasteurii microorganism suspension 600nm
OD value OD600==1.628~1.821.The storage temperature of the Bacillus pasteurii microorganism suspension is 4 DEG C.
3) 10 DEG C of calcium chloride solutions and 10 DEG C of urea liquid mixed configuration reaction solutions are used, and it is 10 DEG C to keep temperature.Its
In, the concentration of the calcium chloride solution is 0.5mol/L, and the concentration of urea liquid is 0.5mol/L.The calcium chloride solution and urine
The volume ratio of plain solution is 1:1, concentration ratio 1:1.
4) 10 DEG C of reaction solutions and 4 DEG C of Bacillus pasteurii microorganism suspensions are taken to prepare to obtain mixed liquor 1.Wherein, it is described
The cumulative volume of mixed liquor 1 is determined according to site area, conduit system output area of section and mixed liquor infusion time.The reaction
The volume ratio of liquid and Bacillus pasteurii microorganism suspension is 20:1.
5) referring to Fig. 2, under Low Night Temperature state, open peristaltic pump 2, by conduit 3 by mixed liquor 1 under assigned rate
Irrigate to desert surface.Mixed liquor 1 is spread into desert surface sand sample 4, and be adsorbed on sand body particle.Wherein, it is described
The infusion time of mixed liquor 1 is controlled within 1h.Under Low Night Temperature state, Bacillus pasteurii activity is relatively low, and mixed liquor 1 is logical
It is uniformly dispersed after entering desert surface.During daytime, with temperature recovery, Bacillus pasteurii activity improves, and decomposing urea activity increases
By force, calcium carbonate largely generates.Compared with prior art, the dispersion degree of calcium carbonate generation is greatly reduced.
6) repeat step 5), until desert surface meets to reinforce target strength.
Embodiment 3：
The present embodiment discloses a kind of microorganism based on temperature conditionss and cures sand optimization method, comprises the following steps：
1) sand sample 4 is prepared using sand rain method.
2) Bacillus pasteurii microorganism suspension is prepared.Wherein, the Bacillus pasteurii microorganism suspension 600nm
OD value OD600==1.628~1.821.The storage temperature of the Bacillus pasteurii microorganism suspension is 4 DEG C.
2) 10 DEG C of calcium chloride solutions and 10 DEG C of urea liquid mixed configuration reaction solutions are used, and it is 10 DEG C to keep temperature.Its
In, the concentration of the calcium chloride solution is 1mol/L, and the concentration of urea liquid is 1mol/L.The calcium chloride solution and urea are molten
The volume ratio of liquid is 1:1, concentration ratio 1:1.
3) 10 DEG C of reaction solutions and 4 DEG C of Bacillus pasteurii microorganism suspensions are taken to prepare to obtain mixed liquor 1.Wherein, it is described
The volume ratio of reaction solution and Bacillus pasteurii microorganism suspension is 10:1.
4) peristaltic pump 2 is opened, is poured into low-temperature mixed liquid 1 in target sand sample 4 under assigned rate by conduit 3.Make to mix
Close liquid 1 to spread into sand sample 4, and be adsorbed on soil body particle.
5) time control is poured within 1h, after pouring into, sand sample 4 is stood 10h.
6) peristaltic pump 2 is opened, injection liquid storing barrel out is extracted the mixed liquor 1 in sand sample 4 by conduit 3.
7) repeat step 4~6), until sand sample 4 meets to reinforce target strength.
Claims (7)
1. a kind of microorganism based on temperature conditionss cures sand optimization method, it is characterised in that comprises the following steps：
1) Bacillus pasteurii microorganism suspension is prepared；Wherein, the Bacillus pasteurii microorganism suspension 600nm light is close
Angle value OD600=1.628~1.821；The storage temperature of the Bacillus pasteurii microorganism suspension is 4 DEG C；
2) 10 DEG C of calcium chloride solutions and 10 DEG C of urea liquid mixed configuration reaction solutions are used, and it is 10 DEG C to keep temperature.Wherein, institute
The concentration for stating calcium chloride solution is 0.25~2mol/L, and the concentration of urea liquid is 0.25~2mol/L；The calcium chloride solution
Volume ratio with urea liquid is 1:1, concentration ratio 1:1；
3) 10 DEG C of reaction solutions and 4 DEG C of Bacillus pasteurii microorganism suspensions are taken to prepare to obtain mixed liquor (1)；Wherein, it is described anti-
The volume ratio for answering liquid and Bacillus pasteurii microorganism suspension is 20:1~10:1；
4) peristaltic pump (2) is opened, is poured into low-temperature mixed liquid (1) in target sand sample (4) under assigned rate by conduit (3)；
Make mixed liquor (1) diffusion into sand sample (4), and be adsorbed on soil body particle；
5) after pouring into, sand sample (4) 10h is stood；
6) peristaltic pump (2) is opened, mixed liquor (1) extraction in sand sample (4) is injected by liquid storing barrel by conduit (3)；
7) repeat step 4)~6), until sand sample (4) meets to reinforce target strength.
2. a kind of microorganism based on temperature conditionss according to claim 2 cures sand optimization method, it is characterised in that：
The internal diameter of the conduit (3) is 3.1mm.
3. a kind of microorganism based on temperature conditionss according to claim 1 cures sand optimization method, it is characterised in that：
In step 1), the Bacillus pasteurii microorganism suspension 600nm OD values OD600=1.75.
4. the microorganism according to claim 1 based on temperature conditionss cures sand optimization method, it is characterised in that：Step
2) in, the concentration of the calcium chloride solution is 2mol/L, and the concentration of urea liquid is 2mol/L.
5. the microorganism according to claim 1 based on temperature conditionss cures sand optimization method, it is characterised in that：Step
4) in, the volume ratio of reaction solution and Bacillus pasteurii microorganism suspension is 15:1.
6. the microorganism according to claim 1 based on temperature conditionss cures sand optimization method, it is characterised in that：Step
4) in, the irrigation rate of mixed liquor (1) is 0.5~1mm/min.
7. the microorganism according to claim 1 based on temperature conditionss cures sand optimization method, it is characterised in that：Step
4) in, the infusion time of mixed liquor (1) is controlled within 1h.
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Cited By (4)
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| CN109881660A (en) * | 2019-02-25 | 2019-06-14 | 河海大学 | A kind of microorganism solidifies the marine structure foundation scouring protection construction method of joint cut-pff wall |
| CN110438975A (en) * | 2019-07-23 | 2019-11-12 | 河海大学 | A kind of microorganism grouting device and construction method for reinforcing deep soft clay |
| CN112779182A (en) * | 2020-12-31 | 2021-05-11 | 武汉工程大学 | Portable microorganism-induced calcium carbonate precipitation kit and application thereof in repairing early cracks of pavement base |
| CN114135294A (en) * | 2021-08-24 | 2022-03-04 | 重庆大学 | Advanced grouting reinforcement method for red sandstone tunnel |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109881660A (en) * | 2019-02-25 | 2019-06-14 | 河海大学 | A kind of microorganism solidifies the marine structure foundation scouring protection construction method of joint cut-pff wall |
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| CN110438975B (en) * | 2019-07-23 | 2021-09-17 | 河海大学 | Microbial grouting device for reinforcing deep soft clay and construction method |
| CN112779182A (en) * | 2020-12-31 | 2021-05-11 | 武汉工程大学 | Portable microorganism-induced calcium carbonate precipitation kit and application thereof in repairing early cracks of pavement base |
| CN114135294A (en) * | 2021-08-24 | 2022-03-04 | 重庆大学 | Advanced grouting reinforcement method for red sandstone tunnel |
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Application publication date: 20180508 |