CN106284280A

CN106284280A - A kind of method utilizing microorganism to prepare calcium carbonate solidification sand

Info

Publication number: CN106284280A
Application number: CN201610828703.2A
Authority: CN
Inventors: 缪林昌; 孙潇昊; 童天志
Original assignee: Southeast University
Current assignee: Southeast University
Priority date: 2016-09-18
Filing date: 2016-09-18
Publication date: 2017-01-04
Anticipated expiration: 2036-09-18
Also published as: CN106284280B

Abstract

The invention discloses a kind of method utilizing microorganism to prepare calcium carbonate solidification sand, under step enters: sand is poured in bottle, make at the bottom of its uniform fold bottle, it is subsequently poured into gelled fluid and cultured bacterium solution, isothermal vibration is cultivated, quantitatively draw part mixed liquor in bottle afterwards every day, then add the gelled fluid of equivalent, be uniformly added into a small amount of sand, after last last from days, obtain the sand post being cured；The present invention has can make full use of microorganism and nutritional solution resource, and technique is simple, and clean environment is with low cost, and finally obtained solidification sand post is overall uniformly without layering, upper and lower even intensity, the advantages such as intensity is bigger.

Description

A kind of method utilizing microorganism to prepare calcium carbonate solidification sand

Technical field

The present invention relates to a kind of method solidifying sand, particularly relate to one and utilize microorganism to prepare calcium carbonate solidification sand The method of soil.

Background technology

Biomineralization is the most universal a kind of natural phenomena, and almost each biology can synthetic mineral.The nearest 2/3rds is calcium ore deposit, and quite a few has cementing function.They are by the vital movement of himself, with surrounding Constantly there is enzymology effect between medium, gradually form calcium carbonate.In recent years, along with the research and development of microorganism in the soil body, The product of the vital movement of the microorganism existed in soil (mainly obtaining energy and the activity of carbon) be used by engineering can Can property conceptually have been achieved for approving widely.Under such overall situation, microorganism curing technology arises at the historic moment.

The metabolite that so-called microorganism solidification utilizes the vital movement of microorganism in soil to generate exactly processes sand etc. and dissipates Dress or weak material, to meet requirement of engineering.Compared with traditional reinforcement technique, bioenergy during microorganism curing technology Instead of mechanical energy, the product of bacteria metabolism instead of chemicals, have clean environment firendly, consume energy low, the soil body is disturbed Dynamic little, process the advantages such as uniform, extremely agree with the strategic objective of sustainable development.

Summary of the invention

Technical problem: it is an object of the invention to provide a kind of method utilizing microorganism to prepare calcium carbonate solidification sand, should Method utilizes enzyme effect, artificial acceleration solidification sand.

Technical scheme: the invention provides a kind of method utilizing microorganism to prepare calcium carbonate solidification sand, including following Step:

1) 10-20g sand is poured in container so that it is uniform fold container bottom；

2) to step 1) described in container in add gelled fluid and bacterium solution, isothermal vibration is cultivated, and obtains mixed liquor；

3) at regular time and quantity from step 2) described in mixed liquor sucking-off a small amount of, add quantitatively in residual mixed liquor the most again Gelled fluid and quantitative sand, obtain after continuous a couple of days calcium carbonate solidification sand.

Wherein:

Described step 2) can also be operated as follows: to step 1) described in container in be subsequently adding bacterium solution, stand Adding calcium chloride solution, add gelled fluid afterwards, isothermal vibration is cultivated.

Step 1) described in the particle diameter of sand less than 2mm.

Step 2) described in gelled fluid, bacterium solution and the volume of sand in bottle for ratio for 2: 1: 1, wherein said gelled fluid is The calcium acetate of 0.5mol/L and every liter of this solution obtain mixed solution after adding the dissolving of 30g carbamide, and described bacterium solution is Pasteur's spore Bacillus, OD600 value is 2.268.

The concentration of described calcium chloride solution is 0.05mol/L, and calcium chloride solution, bacterium solution are 1: 5 with the ratio of gelled fluid: 10；The time of described standing is more than 1 hour.

Step 2) described in the calcium acetate that gelled fluid is 0.5mol/L and every liter of this solution add after 30g carbamide dissolves and mixed Close solution.

Step 2) described in constant temperature be 10～30 DEG C, the frequency of described concussion is 100rpm.

Step 3) described in refer to 1/10th of sucking-off every day mixed liquor at regular time and quantity.

Step 3) described in residual mixed liquor, add quantitative gelled fluid and quantitative sand, add quantitative gelled fluid Volume is 1/9th of residual mixed liquor volume, and the amount of described quantitative sand is identical with the sand amount added before, and this sand Son is evenly laid out on sand before.

Step 3) described in a couple of days after be 14 days after.

Beneficial effect: the present invention makes full use of the resource in nature microorganism resource and nutritional solution, by biological ore deposit Change effect, accelerates deposition calcium carbonate solidification sand, not only aboundresources, and technique is simple, clean environment, with low cost, Solidification sand post entirety obtained by end is the most visibly homogeneous without layering, and upper and lower intensity is the most uniform, and bulk strength is bigger.Room temperature bar Solidification sand technology under part, can be not only used for the reinforcing of soft foundation, it is also possible to for the repairing in crack, regeneration concrete The engineering such as cementing.

Accompanying drawing explanation

Fig. 1 is the stress-strain diagram of the sand post unconfined compression strength test obtaining the present invention.

Detailed description of the invention

The calcium acetate solution that gelled fluid is every liter of 0.5mol/L in lower embodiment add obtain mixing after 30g carbamide dissolves molten Liquid.

Embodiment 1

10～20g sands are poured in bottle so that it is at the bottom of uniform fold bottle, be subsequently poured into 100mL gelled fluid and cultivate with 100mL Good bacterium solution, cultivates then at 20～30 DEG C of isothermal vibrations, draws 10mL mixed liquor afterwards every day, adds 10mL gelled fluid, uniformly Add 10～20g sands, after the most continuous 14 days, obtain the sand post being cured.

Embodiment 2

10～20g sands are poured in bottle so that it is at the bottom of uniform fold bottle, be subsequently poured into 100mL gelled fluid and cultivate with 100mL Good bacterium solution, cultivates then at 10～20 DEG C of isothermal vibrations, draws 10mL mixed liquor afterwards every day, adds 10mL gelled fluid, uniformly Add 10～20g sands, after the most continuous 14 days, obtain the sand post being cured.

Embodiment 3

Embodiment 4

10～20g sands are poured in bottle so that it is at the bottom of uniform fold bottle, be subsequently poured into the cultured bacterium solution of 100mL, stand Pour the calcium chloride solution of the 0.05mol/L of 10mL after 1 hour into, be subsequently added into 100mL gelled fluid, then at 20～30 DEG C of constant temperature shakes Swing cultivation, draw 10mL mixed liquor afterwards every day, add 10mL gelled fluid, be uniformly added into 10～20g sands, the most continuous 14 days After obtain the sand post that is cured.

Claims

1. one kind utilizes the method that calcium carbonate solidification sand is prepared in microorganism, it is characterised in that: the method comprises the following steps:

3) at regular time and quantity from step 2) described in mixed liquor sucking-off a small amount of, in residual mixed liquor, add quantitative glue the most again Lime set and quantitative sand, obtain calcium carbonate solidification sand after continuous a couple of days.

A kind of method utilizing microorganism to prepare calcium carbonate solidification sand, it is characterised in that: described Step 2) be also operated as follows: to step 1) described in container in be subsequently adding bacterium solution, stand and add calcium chloride solution, Adding gelled fluid afterwards, isothermal vibration is cultivated.

A kind of method utilizing microorganism to prepare calcium carbonate solidification sand, it is characterised in that: step 1) particle diameter of the sand described in is less than 2mm.

A kind of method utilizing microorganism to prepare calcium carbonate solidification sand, it is characterised in that: step 2) gelled fluid described in, bacterium solution with the volume of sand in bottle for ratio for 2: 1: 1, wherein said gelled fluid is the vinegar of 0.5mol/L Acid calcium and every liter of this solution obtain mixed solution after adding the dissolving of 30g carbamide, and described bacterium solution is Bacillus pasteurii, OD600 value It is 2.268.

A kind of method utilizing microorganism to prepare calcium carbonate solidification sand, it is characterised in that: described The concentration of calcium chloride solution be 0.05mol/L, calcium chloride solution, bacterium solution are 1: 5: 10 with the ratio of gelled fluid；Described is quiet The time put is more than 1 hour.

A kind of method utilizing microorganism to prepare calcium carbonate solidification sand, it is characterised in that: Step 2) described in the calcium acetate that gelled fluid is 0.5mol/L and every liter of this solution add after 30g carbamide dissolves and obtain mixed solution.

A kind of method utilizing microorganism to prepare calcium carbonate solidification sand, it is characterised in that: Step 2) described in constant temperature be 10～30 DEG C, the frequency of described concussion is 100rpm.

A kind of method utilizing microorganism to prepare calcium carbonate solidification sand, it is characterised in that: step 3) 1/10th of sucking-off every day mixed liquor are referred at regular time and quantity described in.

A kind of method utilizing microorganism to prepare calcium carbonate solidification sand, it is characterised in that: step 3) adding quantitative gelled fluid and quantitative sand in residual mixed liquor described in, the volume adding quantitative gelled fluid is mixed for residue Close that liquid is long-pending 1/9th, the amount of described quantitative sand is identical with the sand amount added before, and this sand evenly laid out On sand before.

A kind of method utilizing microorganism to prepare calcium carbonate solidification sand, it is characterised in that: step Rapid 3) after being 14 days after a couple of days described in.