CN106284280B

CN106284280B - A method of calcium carbonate solidification sand is prepared using microorganism

Info

Publication number: CN106284280B
Application number: CN201610828703.2A
Authority: CN
Inventors: 缪林昌; 孙潇昊; 童天志
Original assignee: Southeast University
Current assignee: Southeast University
Priority date: 2016-09-18
Filing date: 2016-09-18
Publication date: 2019-10-11
Anticipated expiration: 2036-09-18
Also published as: CN106284280A

Abstract

The method that calcium carbonate solidifies sand is prepared using microorganism the invention discloses a kind of, under step enters: sand is poured into bottle, make its uniform fold bottom of bottle, it is subsequently poured into gelled fluid and cultured bacterium solution, isothermal vibration culture, it is quantitative daily later to draw part mixed liquor in bottle, then add the gelled fluid of equivalent, be uniformly added into a small amount of sand, the sand column being cured after last last from days；The present invention has and can make full use of microorganism and nutrient solution resource, and simple process, clean environment is low in cost, and finally obtained solidification sand column is integrally uniformly without layering, the advantages that upper and lower even intensity, intensity is larger.

Description

A method of calcium carbonate solidification sand is prepared using microorganism

Technical field

The present invention relates to a kind of methods for solidifying sand, prepare calcium carbonate solidification sand using microorganism more particularly to a kind of The method of soil.

Background technique

Biomineralization is a kind of very universal natural phenomena, and almost each biology can synthetic mineral.It is wherein close Three/second is that calcium mine, and quite a few is with cementing function.They are by its own vital movement, with ambient enviroment Enzymeization effect constantly occurs between medium, gradually forms calcium carbonate.In recent years, with the research and development of microorganism in the soil body, In soil the product of the vital movement activity of energy and carbon (mainly obtain) of already existing microorganism be can used in engineering Energy property conceptually has been achieved for widely approving.Under such overall situation, microorganism curing technology comes into being.

So-called microorganism is solidified the metabolite processing sand etc. for being exactly to be generated using the vital movement of microorganism in soil and dissipated Dress or weak material, to meet requirement of engineering.Compared with traditional reinforcement technique, biological energy source during microorganism curing technology Instead of mechanical energy, the product of bacteria metabolism is instead of chemicals, and with clean and environmental protection, energy consumption is low, disturbs to the soil body It moves small, handles the advantages that uniform, extremely agree with the strategic objective of sustainable development.

Summary of the invention

Technical problem: preparing the method that calcium carbonate solidifies sand using microorganism the object of the present invention is to provide a kind of, should Method is acted on using biological enzymeization, and artificial acceleration solidifies sand.

Technical solution: the method that calcium carbonate solidifies sand is prepared using microorganism the present invention provides a kind of, including following Step:

1) 10-20g sand is poured into container, makes its uniform fold container bottom；

2) gelled fluid is added into the container described in step 1) and bacterium solution, isothermal vibration culture obtains mixed liquor；

3) it is sucked out on a small quantity, is added again into residual mixed liquor later quantitative from the mixed liquor described in step 2) at regular time and quantity Gelled fluid and quantitative sand, obtained after continuous a couple of days calcium carbonate solidification sand.

Wherein:

The step 2) can also be operated as follows: bacterium solution being then added into the container described in step 1), stands Calcium chloride solution is added, gelled fluid, isothermal vibration culture are added later.

The partial size of sand described in step 1) is less than 2mm.

The volume of sand is than being 2: 1: 1, wherein the gelled fluid is in gelled fluid described in step 2), bacterium solution and bottle The calcium acetate of 0.5mol/L and every liter of solution obtain mixed solution after adding 30g urea to dissolve, and the bacterium solution is Pasteur's gemma Bacillus, OD600 value are 2.268.

The concentration of the calcium chloride solution is 0.05mol/L, and the ratio of calcium chloride solution, bacterium solution and gelled fluid is 1: 5: 10；The time of the standing is greater than 1 hour.

Gelled fluid described in step 2) be 0.5mol/L calcium acetate and every liter of solution add 30g urea dissolve after mixed Close solution.

Constant temperature described in step 2) is 10~30 DEG C, and the frequency of the concussion is 100rpm.

Refer at regular time and quantity described in step 3) and 1/10th of mixed liquor is sucked out daily.

Quantitative gelled fluid and quantitative sand is added described in step 3) into residual mixed liquor, quantitative gelled fluid is added Volume is 1/9th of residual mixed liquor volume, and the amount of the quantitative sand is identical as the sand amount added before, and the sand Son is evenly laid out on sand before.

It is after 14 days after a couple of days described in step 3).

The utility model has the advantages that the present invention makes full use of the resource in nature microorganism resource and nutrient solution, pass through the mine of biology Change effect accelerates deposition calcium carbonate to solidify sand, not only resourceful, but also simple process, and clean environment is low in cost, most The whole also visibly homogeneous no layering of obtained solidification sand column eventually, upper and lower intensity is also very uniform, and integral strength is larger.Room temperature item Solidification sand technology under part, can be not only used for the reinforcing of soft foundation, can be also used for the repairing in crack, regeneration concrete The engineerings such as cementing.

Detailed description of the invention

Fig. 1 is the stress-strain diagram of the sand column unconfined compression strength test obtained to the present invention.

Specific embodiment

The calcium acetate solution that gelled fluid in lower embodiment is every liter of 0.5mol/L obtains mixing molten after adding 30g urea to dissolve Liquid.

Embodiment 1

10~20g sand is poured into bottle, its uniform fold bottom of bottle is made, 100mL gelled fluid is subsequently poured into and 100mL is cultivated Good bacterium solution draws 10mL mixed liquor then at 20~30 DEG C of isothermal vibration cultures daily later, adds 10mL gelled fluid, uniformly 10~20g sand, the sand column being finally cured after continuous 14 days is added.

Embodiment 2

10~20g sand is poured into bottle, its uniform fold bottom of bottle is made, 100mL gelled fluid is subsequently poured into and 100mL is cultivated Good bacterium solution draws 10mL mixed liquor then at 10~20 DEG C of isothermal vibration cultures daily later, adds 10mL gelled fluid, uniformly 10~20g sand, the sand column being finally cured after continuous 14 days is added.

Embodiment 3

Embodiment 4

10~20g sand is poured into bottle, its uniform fold bottom of bottle is made, is subsequently poured into the cultured bacterium solution of 100mL, is stood The calcium chloride solution that the 0.05mol/L of 10mL is poured into after 1 hour is subsequently added into 100mL gelled fluid, shakes then at 20~30 DEG C of constant temperature Culture is swung, draws 10mL mixed liquor daily later, 10mL gelled fluid is added, is uniformly added into 10~20g sand, it is continuous 14 days last The sand column being cured afterwards.

Claims

1. a kind of prepare the method that calcium carbonate solidifies sand using microorganism, it is characterised in that: method includes the following steps:

1) sand by 10-20g partial size less than 2mm pours into container, makes its uniform fold container bottom；

2) the Bacillus pasteurii bacterium solution that OD600 value is 2.268 is added into the container described in step 1), stands and is greater than 1 hour, 0.05mol/L calcium chloride solution is added, gelled fluid is added later, the isothermal vibration under 10~30 DEG C and 100rpm of oscillation frequency Culture；

Wherein, the gelled fluid be 0.5mol/L calcium acetate and every liter of solution add 30g urea dissolve after obtain mixed solution, The ratio of calcium chloride solution, bacterium solution and gelled fluid is 1: 5: 10；In the gelled fluid, bacterium solution and bottle the volume of sand be than for 2:1:1；

3) 1/10th are sucked out from the mixed liquor described in step 2) at regular time and quantity, is added again into residual mixed liquor later quantitative Gelled fluid and quantitative sand, the volume that quantitative gelled fluid is added is 1/9th of residual mixed liquor volume, and described quantifies The amount of sand is identical as the sand amount added before, and the sand is evenly laid out on sand before；Carbon is obtained after continuous 14 days Sour calcium solidifies sand.