CN105689383A

CN105689383A - Method for removing notoginsenoside autotoxins through ozone

Info

Publication number: CN105689383A
Application number: CN201610048690.7A
Authority: CN
Inventors: 杨敏; 朱书生; 张潇丹; 尹兆波; 钏有聪; 罗丽芬; 许彦国; 江冰冰; 刘屹湘; 黄惠川; 何霞红; 朱有勇
Original assignee: Yunnan Agricultural University
Current assignee: Yunnan Agricultural University
Priority date: 2016-01-25
Filing date: 2016-01-25
Publication date: 2016-06-22

Abstract

The invention relates to a method for removing notoginsenoside autotoxins through ozone and belongs to the technical field of plant protection. According to the technical scheme, ozone gas is uniformly and continuously introduced into a continuous cropping medium culture solution and soil where panax notoginseng is planted, and the notoginsenoside autotoxins are removed under the conditions that the generation speed of the ozone gas is 32 g/h, the contact time of continuous cropping media and the ozone is longer than 5 min, and ventilation and air-curing are conducted for 2 or more hours after treatment. The method has the beneficial effects that 1, the saponin autotoxins in the continuous cropping soil for the panax notoginseng are treated through the ozone, the saponin autotoxins in the continuous cropping soil can be effectively removed, a panax notoginseng continuous cropping obstacle is overcome, and environment friendliness and zero residue are achieved; and 2, the probability of collaborative pathopoiesis of the autotoxins and root rot funguses in the panax notoginseng growing process is reduced, the usage amount of pesticides is reduced, and the method has important significance in improving the quality of the panax notoginseng, reducing pesticide residue of the panax notoginseng, and protecting a panax notoginseng genuine producing area.

Description

A kind of ozone eliminates the arasaponin class method from poisonous substance matter

Technical field:

The present invention relates to a kind of ozone and eliminate the arasaponin class method from poisonous substance matter, platymiscium resist technology field。

Background technology:

Radix Notoginseng is one of large rare Chinese medicine of China, account for Yunnan Province's medical material output value more than half, but notoginseng obstacle is serious。Existing research shows, planted the soil of Radix Notoginseng after 10 to 20 years can not continuous cropping, continuous cropping obstacle has become as the principal element that restriction Radix Notoginseng industry develops in a healthy way。For many years, numerous scholars expand deep research around the reason causing notoginseng obstacle, it is believed that main the infecting with pine root fungus of continuous cropping obstacle, relevant from factors such as the changes of the accumulation of poisonous substance matter, the deterioration of soil physical and chemical property and soil microflora。The research of this project team shows, pine root fungus and the accumulation from poisonous substance matter are to cause notoginseng obstacle unassailable key factor of a specified duration。Research finds that Radix Notoginseng is secreted into the saponins material in rhizosphere soil and the growth of notoginseng root rot bacterium has obvious growth promoting function, and Panax notoginseng seeds sprouting and root cell are had significantly from cytotoxic activity by these saponins materials simultaneously。This shows that saponin is the distinctive allelochemical of Radix Notoginseng, is the key factor causing notoginseng obstacle。Therefore, safe, ecological arasaponin class is probed into significant for capturing notoginseng obstacle from the removing method of poisonous substance matter。

Ozone provided by the invention eliminates the arasaponin class method from poisonous substance matter, by literature search, has no the open source literature report identical with the present invention。

The content of invention:

It is an object of the invention to overcome the deficiency of prior art, and provide a kind of ozone to eliminate the arasaponin class method from poisonous substance matter。

The present invention is directed to the saponins that causes notoginseng obstacle from poisonous substance matter, utilize the Strong oxdiative ability of ozone and the characteristic of environmentally safe to Panax notoginseng Growth medium (soil or culture fluid) carries out innoxious clearing up from poisonous substance matter。

The technical scheme is that and ozone gas uniformly continuous is passed in the continuous cropping medium culture fluid and soil planting Radix Notoginseng, ozone gas generation rate be 32g/h, continuous cropping medium and ozone contact time more than 5min, after process under illumination when ventilation dry in the sun more than 2 hours elimination arasaponin class from poisonous substance matter。

The beneficial effects of the present invention is:

1, utilize the saponins in ozonization notoginseng soil from poisonous substance matter, can effectively eliminate the saponins in continuous cropping soil from poisonous substance matter, overcome notoginseng obstacle and environmental protection noresidue。

2, reducing in Panax notoginseng Growth process and work in coordination with pathogenic probability from poisonous substance matter and pine root fungus, what decrease pesticide makes consumption, to improving Radix Notoginseng quality, reduce Radix Notoginseng pesticide residues, protecting Radix Notoginseng Genuine producing area to have important meaning。

Detailed description of the invention:

Embodiment one: clear up in culture fluid arasaponin class from poisonous substance matter with ozone

Collecting the culture fluid having cultivated two months Radix Notoginseng seedlings in culture pond (20cm × 10cm × 10cm), the ozone released by ozonator is ozonation aerated by carrying out in silicone rubber tube introducing culture fluid, and speed is 32g/h。Comparison is then for passing into the air of equivalent in culture fluid。After processing 3min, 5min, 10min, 30min, 60min, place 2 hours under illumination condition。Then, separately sampled culture fluid carries out from poisonous substance matter residue detection, repeats 3 times every time。Continue the Radix Notoginseng culture fluid after above-mentioned process respectively to cultivate Radix Notoginseng seedling, add up Radix Notoginseng after 15 days and deposit Seedling rate。It is shown that just can significantly clear up after ozonization culture fluid 5min in culture fluid from poisonous substance matter, improve the Seedling rate of depositing (table 1) of Radix Notoginseng。

Table 1 ozonization different time is on the clearing up effect and Radix Notoginseng is deposited the impact of Seedling rate from poisonous substance matter of arasaponin class in culture fluid

Embodiment two: with ozone gas process notoginseng soil soil from poisonous substance matter

Being continually fed into ozone gas in continuous cropping soil, its speed is 32g/h, and comparison then passes into equivalent air。Make soil after uniform treatment 3min, 5min, 10min, 30min, 60min in ozone atmosphere, ventilation dry in the sun 2 hours under illumination。Then from the residual quantity of poisonous substance matter in sampling detection soil, all the other soil sowing Panax notoginseng seeds, observation Radix Notoginseng emerges and deposits growth of cereal crop seedlings condition。It is shown that can substantially eliminate after ozonization 5min in continuous cropping soil from poisonous substance matter, improve the Seedling rate of depositing (table 2) of Radix Notoginseng。

Table 2 ozonization different time is on continuous cropping soil emergence rate and the impact depositing Seedling rate

Claims

1. one kind eliminates the arasaponin class method from poisonous substance matter with ozone, it is characterized in that the method is: pass in the continuous cropping medium culture fluid and soil planting Radix Notoginseng by ozone gas uniformly continuous, ozone gas generation rate be 32g/h, continuous cropping medium and ozone contact time more than 5min, after process under illumination when ventilation dry in the sun more than 2 hours elimination arasaponin class from poisonous substance matter。