CN107646697A - A kind of mating system of scythian lamb rhizome spore - Google Patents
A kind of mating system of scythian lamb rhizome spore Download PDFInfo
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- CN107646697A CN107646697A CN201711123886.9A CN201711123886A CN107646697A CN 107646697 A CN107646697 A CN 107646697A CN 201711123886 A CN201711123886 A CN 201711123886A CN 107646697 A CN107646697 A CN 107646697A
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- culture
- spore
- sporangium
- scythian lamb
- lamb rhizome
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
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Abstract
The invention discloses a kind of mating system of scythian lamb rhizome spore, pluck the accessory pinna with sporangiorus on wild Cibotium barometz plant, collect sporangium, sterilization is dried after rinsing, it is collected in sterile bag, then by after sterile bag deepfreeze 340 380 days, sporangium is carried out disinfection with 70% 75% alcohol, spore germination culture medium is then seeded to and carries out sprouting culture；After original foliage is grown, original foliage is seeded to cellar culture matrix and carries out gametophyte conversion culture；After gametophyte grows the 3rd leaf, transfer culture tank to interior, open culture cover and carry out hardening culture, the soil that then has additional nutrients carries out field transplanting adaptation, finally carries out field transplanting.The cultural method operating process of the scythian lamb rhizome spore of the present invention is simple, cultivates scythian lamb rhizome seedling by two-step method, saves cost, and seedling early growth is neat, is adapted to large-scale planting.
Description
Technical field
The present invention relates to field of plant growing technology, and in particular to a kind of mating system of scythian lamb rhizome spore.
Background technology
Scythian lamb rhizome (Cibotium barometz (L.) J.Sm.) is fern Dicksoniaceae plant,《Dictionary of medicinal plant》In
Record：Scythian lamb rhizome is born in acid soil at foot of the hill limes marginis, or sylvan life the moon.It is distributed Southwestern China, south, the southeast and Henan, lake
The ground such as north, main product Sichuan, Fujian, Zhejiang.In addition, the ground such as Guangxi, Guangdong, Guizhou, Jiangxi, Hubei also produces.
It is Chinese medicine rhizoma cibotii that scythian lamb rhizome, which dries plucked rhizome, first recorded in《Sheng Nong's herbal classic》, go through version《Chinese Pharmacopoeia》
Record, have filling liver kidney, strengthening the bones and muscles, relax channels and collaterals, dehumidifying pain and diuresis function, for treat waist and leg ache, it is numb in every limb,
The diseases such as hemiplegia, leukorrhea seminal emission, metrorrhagia；Animal experiment proves that the young pilose antler of rhizoma cibotii to scar tissue, liver, spleen damage
The evil traumatic hemorrhage such as property and extraction has preferable anastalsis, and its effect is rapid compared with gelfoam etc., meanwhile, rhizoma cibotii young pilose antler is seemingly
It can gradually be assimilated by tissue, also there are increased platelets counts；Modern study is found：Scythian lamb rhizome extract has suppression
Make a variety of cancer cells, anti-inflammatory, antibacterial and other effects.
Due to a variety of Chinese patent drugs containing rhizoma cibotii of domestic pharmacy corporation, the demand to rhizoma cibotii is increased, is caused among the people crazy
Mad excavation, wild resource is day by day rare, and the seedling breeding of artificial growth rhizoma cibotii is complicated in addition, uses root-like stock cutting section propagation nursery
Cost is high, cycle length, is not suitable for promoting.
The content of the invention
It is an object of the invention to provide a kind of mating system of scythian lamb rhizome spore, the quick of scythian lamb rhizome can be realized
Breed, realize large-scale planting.
In order to realize the above-mentioned purpose of the present invention, spy uses following technical scheme：A kind of side of breeding of scythian lamb rhizome spore
Method, comprise the following steps：
(1) collection of spore：The accessory pinna with sporangiorus on wild Cibotium barometz plant is plucked, is put naturally cloudy in clean bag
It is dry, the removal of impurity is gone after sporangium comes off naturally, collects sporangium, after 0.1% mercuric chloride solution effect 4-6 minutes, then is used
Aseptic water washing 3-5 times, it is collected in after drying in sterile bag；
(2) pre-treatment is cultivated：The sterile bag containing sporangium that step (1) obtains is placed in 2-6 DEG C of preservation 340-380
My god, the sporangium of low-temperature preservation is carried out disinfection the 20-40 seconds with 70%-75% alcohol；To spore germination culture medium and routine
Culture matrix enters horizontal high voltage moist heat sterilization；
(3) sprout and cultivate：The sporangium that step (2) disinfects is seeded to the tissue culture tank equipped with spore germination culture medium
In, sprouting culture, 22-28 DEG C of temperature, daily 2000-2500Lx illumination 10-12 hours are carried out in illumination box；Work as prophyll
After body is grown, original foliage is seeded in the culture tank containing cellar culture matrix, gametophyte is carried out in illumination box and is turned
Change culture, 22-28 DEG C of temperature, daily 2000-2500Lx illumination 10-12 hours；
(4) hardening and transplanting：After gametophyte grows the 3rd leaf, transfer culture tank to interior, open culture cover and carry out
Hardening culture, daily spraying 1-2 time holding humidity, hardening had additional nutrients after 7-10 days soil carry out field transplanting adaptation, daily spray
1-2 holding humidity of mist, transplanting carry out field transplanting after adapting to 7-10 days；
Further technical scheme, the spore germination culture medium include 1/4 modified MS medium, sucrose 1-3%, agar
0.5-0.8%；
Further technical scheme, the cellar culture matrix include vermiculite, CNT and nutrient solution, and the vermiculite is
Vermiculite through citric acid immersion treatment, the quality parts ratio of the vermiculite and CNT is 4:1, the nutrient solution includes 1/2
Modified MS medium, sucrose 1-3%；
Further technical scheme, the spore germination culture medium also include sterile 6-BA0.02-0.1mg/L and sterile
IAA0.02-0.1mg/L, the nutrient solution also include sterile GA30.02-0.1mg/L and sterile 6-BA0.02-0.1mg/L；
Further technical scheme, the composition improved in the modified MS medium are：KNO3It is adjusted to 600-
700mg/L, (NH)4NO3It is adjusted to 500-600mg/L；
Further technical scheme, described to sprout culture for 60-90 days, the gamete conversion culture is 45-90 days.
Compared with prior art, the beneficial effects of the invention are as follows：The scythian lamb rhizome Spore cultivation method of the present invention, was operated
Journey is simple, and scythian lamb rhizome seedling is cultivated by two-step method, reduces repeatedly injury of the inoculation to scythian lamb rhizome seedling, is with vermiculite etc.
Matrix, reduce the hardening before scythian lamb rhizome transplantation of seedlings and change basin, realize that the matrix that transplanting is transformed into from gametophyte rationally utilizes, greatly
Amount saves cost；This method does not injure the parent of wild plant, does not influence the growth of plant, and can obtain a large amount of physiological ages
Unanimously, neat seedling is grown, is adapted to scale breeding and cultivation；And this method is based on zoogamy, the seedling of acquisition
The infection of virus-free or other diseases, plant vitality is strong, robust growth, and survival rate is high；A large amount of wild Cibotium barometzs are avoided simultaneously
The excavation of ridge resource, country is protected to treasure fern resource, beneficial to the protection of wild scythian lamb rhizome.
Embodiment
Technical scheme is described in detail below in conjunction with embodiment, the embodiment is the excellent of the present invention
Embodiment is selected, the present invention is merely to illustrate and limits the scope of the present invention, it will be understood by those skilled in the art that based on the present invention's
Technical scheme, the other technologies scheme obtained on the premise of no creativeness both fall within protection scope of the present invention.Embodiment
The middle unreceipted production firm person of agents useful for same or instrument, it is the conventional products that can be obtained by commercially available purchase.
Embodiment 1
A kind of mating system of scythian lamb rhizome spore, comprises the following steps：
(1) collection of spore：The accessory pinna with sporangiorus on wild Cibotium barometz plant is plucked, is put naturally cloudy in clean bag
It is dry, the removal of impurity is gone after sporangium comes off naturally, collects sporangium, after being acted on 4 minutes with 0.1% mercuric chloride solution, then with nothing
Bacterium water rinses 3 times, is collected in after drying in sterile bag；
(2) pre-treatment is cultivated：The sterile bag containing sporangium that step (1) obtains is placed in 2 DEG C of preservations 340 days, used
75% alcohol carries out disinfection 40 seconds to the sporangium of low-temperature preservation；Spore germination culture medium and cellar culture matrix are carried out high
Press moist heat sterilization；
(3) sprout and cultivate：The sporangium that step (2) disinfects is seeded to the tissue culture tank equipped with spore germination culture medium
In, sprouting culture, 22 DEG C of temperature, daily 2000-2500Lx illumination 12 hours are carried out in illumination box；When original foliage is grown
Afterwards, original foliage is seeded in the culture tank containing cellar culture matrix, gametophyte conversion culture is carried out in illumination box,
22 DEG C of temperature, daily 2000-2500Lx illumination 12 hours；
(4) hardening and transplanting：After gametophyte grows the 3rd leaf, transfer culture tank to interior, open culture cover and carry out
Hardening culture, daily spraying 1-2 time holding humidity, hardening had additional nutrients after 7-10 days soil carry out field transplanting adaptation, daily spray
1-2 holding humidity of mist, transplanting carry out field transplanting after adapting to 7-10 days.
Embodiment 2
A kind of mating system of scythian lamb rhizome spore, comprises the following steps：
(1) collection of spore：The accessory pinna with sporangiorus on wild Cibotium barometz plant is plucked, is put naturally cloudy in clean bag
It is dry, the removal of impurity is gone after sporangium comes off naturally, collects sporangium, after being acted on 6 minutes with 0.1% mercuric chloride solution, then with nothing
Bacterium water rinses 5 times, is collected in after drying in sterile bag；
(2) pre-treatment is cultivated：The sterile bag containing sporangium that step (1) obtains is placed in 6 DEG C of preservations 380 days, used
70% alcohol carries out disinfection 40 seconds to the sporangium of low-temperature preservation；Spore germination culture medium and cellar culture matrix are carried out high
Press moist heat sterilization；
(3) sprout and cultivate：The sporangium that step (2) disinfects is seeded to the tissue culture tank equipped with spore germination culture medium
In, sprouting culture, 28 DEG C of temperature, daily 2000-2500Lx illumination 12 hours are carried out in illumination box；When original foliage is grown
Afterwards, original foliage is seeded in the culture tank containing cellar culture matrix, gametophyte conversion culture is carried out in illumination box,
28 DEG C of temperature, daily 2000-2500Lx illumination 12 hours；
(4) hardening and transplanting：After gametophyte grows the 3rd leaf, transfer culture tank to interior, open culture cover and carry out
Hardening culture, daily spraying 1-2 time holding humidity, hardening had additional nutrients after 7-10 days soil carry out field transplanting adaptation, daily spray
1-2 holding humidity of mist, transplanting carry out field transplanting after adapting to 7-10 days.
Embodiment 3
A kind of mating system of scythian lamb rhizome spore, comprises the following steps：
(1) collection of spore：The accessory pinna with sporangiorus on wild Cibotium barometz plant is plucked, is put naturally cloudy in clean bag
It is dry, the removal of impurity is gone after sporangium comes off naturally, collects sporangium, after being acted on 5 minutes with 0.1% mercuric chloride solution, then with nothing
Bacterium water rinses 5 times, is collected in after drying in sterile bag；
(2) pre-treatment is cultivated：The sterile bag containing sporangium that step (1) obtains is placed in 4 DEG C of preservations 360 days, used
75% alcohol carries out disinfection 30 seconds to the sporangium of low-temperature preservation；Spore germination culture medium and cellar culture matrix are carried out high
Press moist heat sterilization；
(3) sprout and cultivate：The sporangium that step (2) disinfects is seeded to the tissue culture tank equipped with spore germination culture medium
In, sprouting culture, 25 DEG C of temperature, daily 2000-2500Lx illumination 11 hours are carried out in illumination box；When original foliage is grown
Afterwards, original foliage is seeded in the culture tank containing cellar culture matrix, gametophyte conversion culture is carried out in illumination box,
25 DEG C of temperature, daily 2000-2500Lx illumination 11 hours；
(4) hardening and transplanting：After gametophyte grows the 3rd leaf, transfer culture tank to interior, open culture cover and carry out
Hardening culture, 2 holding humidity of spraying daily, the hardening soil that had additional nutrients after 7-10 days carry out field transplanting adaptation, spray 2 daily
Secondary holding humidity, transplanting carry out field transplanting after adapting to 7-10 days.
The spore germination culture medium includes 1/4 modified MS medium, sucrose 3%, agar 0.7%；
The cellar culture matrix includes vermiculite, CNT and nutrient solution, and the vermiculite is through citric acid immersion treatment
Vermiculite, the quality parts ratio of the vermiculite and CNT is 4:1, the nutrient solution includes 1/2 modified MS medium, sucrose
3%；
The spore germination culture medium also includes sterile 6-BA0.04mg/L and sterile IAA0.02mg/L, the nutrient solution
Also include sterile GA30.04mg/L and sterile 6-BA0.02mg/L；
The composition improved in the modified MS medium is：KNO3650mg/L is adjusted to, (NH)4NO3It is adjusted to 550mg/
L；
Described to sprout culture for 60-90 days, the gamete conversion culture is 45-90 days.
Claims (7)
1. a kind of mating system of scythian lamb rhizome spore, it is characterised in that comprise the following steps：
(1) collection of spore：The accessory pinna with sporangiorus on wild Cibotium barometz plant is plucked, puts in clean bag and dries in the shade naturally,
Go the removal of impurity after sporangium comes off naturally, collect sporangium, after 0.1% mercuric chloride solution effect 4-6 minutes, then with sterile
Water rinses 3-5 times, is collected in after drying in sterile bag；
(2) pre-treatment is cultivated：The sterile bag containing sporangium that step (1) obtains is placed in 2-6 DEG C of preservation 340-380 days, used
70%-75% alcohol carries out disinfection the 20-40 seconds to the sporangium of low-temperature preservation；To spore germination culture medium and conventional medium
Matter enters horizontal high voltage moist heat sterilization；
(3) sprout and cultivate：The sporangium that step (2) disinfects is seeded in the tissue culture tank equipped with spore germination culture medium,
Sprouting culture, 22-28 DEG C of temperature, daily 2000-2500Lx illumination 10-12 hours are carried out in illumination box；Work as original foliage
After growing, original foliage is seeded in the culture tank containing cellar culture matrix, gametophyte conversion is carried out in illumination box
Culture, 22-28 DEG C of temperature, daily 2000-2500Lx illumination 10-12 hours；
(4) hardening and transplanting：After gametophyte grows the 3rd leaf, transfer culture tank to interior, open culture cover and carry out hardening
Culture, daily spraying 1-2 time holding humidity, hardening had additional nutrients after 7-10 days soil progress field transplanting adaptation, spray 1-2 daily
Secondary holding humidity, transplanting carry out field transplanting after adapting to 7-10 days.
A kind of 2. mating system of scythian lamb rhizome spore according to claim 1, it is characterised in that the spore germination training
Supporting base includes 1/4 modified MS medium, sucrose 1-3%, agar 0.5-0.8%.
A kind of 3. mating system of scythian lamb rhizome spore according to claim 1, it is characterised in that the conventional medium
Matter includes vermiculite, CNT and nutrient solution, and the vermiculite is the vermiculite through citric acid immersion treatment, vermiculite and the carbon nanometer
The quality parts ratio of pipe is 4:1, the nutrient solution includes 1/2 modified MS medium, sucrose 1-3%.
A kind of 4. mating system of scythian lamb rhizome spore according to claim 2, it is characterised in that the spore germination training
Foster base also includes sterile 6-BA0.02-0.1mg/L and sterile IAA0.02-0.1mg/L.
5. the mating system of a kind of scythian lamb rhizome spore according to claim 3, it is characterised in that the nutrient solution also wraps
Include sterile GA30.02-0.1mg/L and sterile 6-BA0.02-0.1mg/L.
6. according to a kind of mating system of any described scythian lamb rhizome spore in claim 2-5, it is characterised in that described to change
The composition improved in good MS culture mediums is：KNO3600-700mg/L is adjusted to, (NH)4NO3It is adjusted to 500-600mg/L.
A kind of 7. mating system of scythian lamb rhizome spore according to claim 1, it is characterised in that it is described sprouting culture be
60-90 days, the gamete conversion culture was 45-90 days.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109042115A (en) * | 2018-07-17 | 2018-12-21 | 广州中量药业有限公司 | A kind of mating system of Cibotium barometz spore |
| CN109699495A (en) * | 2019-02-12 | 2019-05-03 | 云南农业大学 | A method of improving scythian lamb rhizome spore germination rate |
| CN112021178A (en) * | 2020-09-01 | 2020-12-04 | 中国科学院植物研究所 | Method for promoting germination of Alsophila spinulosa spores by using multiwalled carbon nanotubes |
| CN117089510A (en) * | 2023-08-30 | 2023-11-21 | 毕节市中药研究所 | Culture medium combination for inducing germination and proliferation of Cibotium barometz spores, and method and application for inducing protophylls |
-
2017
- 2017-11-14 CN CN201711123886.9A patent/CN107646697A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109042115A (en) * | 2018-07-17 | 2018-12-21 | 广州中量药业有限公司 | A kind of mating system of Cibotium barometz spore |
| CN109699495A (en) * | 2019-02-12 | 2019-05-03 | 云南农业大学 | A method of improving scythian lamb rhizome spore germination rate |
| CN112021178A (en) * | 2020-09-01 | 2020-12-04 | 中国科学院植物研究所 | Method for promoting germination of Alsophila spinulosa spores by using multiwalled carbon nanotubes |
| CN112021178B (en) * | 2020-09-01 | 2021-10-22 | 中国科学院植物研究所 | Method for promoting germination of Alsophila spinulosa spores by using multiwalled carbon nanotubes |
| CN117089510A (en) * | 2023-08-30 | 2023-11-21 | 毕节市中药研究所 | Culture medium combination for inducing germination and proliferation of Cibotium barometz spores, and method and application for inducing protophylls |
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