CN105685474A

CN105685474A - Bacillus subtilis-containing feed additive and preparation method thereof

Info

Publication number: CN105685474A
Application number: CN201610075308.1A
Authority: CN
Inventors: 刘旭辉
Original assignee: Liaoning Xu Hui Biological Technology Co Ltd
Current assignee: Liaoning Xu Hui Biological Technology Co Ltd
Priority date: 2016-02-03
Filing date: 2016-02-03
Publication date: 2016-06-22

Abstract

The invention relates to a bacillus subtilis-containing feed additive. The bacillus subtilis-containing feed additive is prepared from the following raw materials in parts by weight: 15-20 parts of bacillus subtilis, 5-15 parts of laetiporus sulphureus, 5-15 parts of lentinus edodes, 5-15 parts of agaric fungus, 5-15 parts of hericium erinaceus fungus and 5-15 parts of agrocybe aegerita fungus. The micro-ecological feed additive has the advantages of having disease resistance and pestilence resistance, improving immunity and degrading various nocuous toxins contained in feeds, additives and vaccines. The feed additive is prepared from pure natural wild microorganism fungi by artificial culture and is a substance contributing to enteric microorganism balance; and the feed additive contains a physiologically active microorganism, can be multiplied by the stomach and intestines or by colonization in the intestinal tract, and can adjust the intestinal flora to improve the immunity of the organism.

Description

A kind of feed additive containing bacillus subtilis and preparation method thereof

Technical field

The present invention relates to a kind of feed additive, particularly relate to a kind of microbial forage additive and preparation method thereof。

Background technology

Over nearly more than 10 years, China's its feeding industry is sustainable growth, and poultry property accounts for the proportion of about 30% in the general production value of agriculture, and meat, egg total output occupy first place in the world, and wherein the ratio shared by Carnis Sus domestica and Carnis Gallus domesticus is up to about 40%。But because China is populous, meat, egg, milk per capita consuming level relatively low。Therefore, continue the animal husbandry of development China, be the needs of national economy, be also the needs uplifted the people's living standard。China's sustainable development animal husbandry is subject to the serious restriction of feedstuff shortage, needs 2.5Kg grain to calculate by often producing 0.5Kg meat, increases production 10,000,000 tons of meats and needs consume 5,000,000 ton-grain foods, even if raising grain-saving type poultry, it is also desirable to extremely large amount of feedstuff。At present, most of plants, large-area high-density breeding, create substantial amounts of Organic substance and harmful substance, result in the severe exacerbation of environment, toxic and side effects and drug residue and had influence on human health。Therefore the simple way relying on the raising quantity expanding poultry to improve production of meat, is difficult to。Improve and cultivate excellent livestock and poultry species, improving feeding and management condition, being no doubt the important measures improving animal husbandry quality and benefits, but rely on scientific and technological advances, effectively controlling livestock and poultry, reducing death loss, be then the approach of another practicable。According to investigations, epidemic disease China's pig mortality rate average out to caused 10% 12%, chicken be 20% 30%, annual economic button loses up to 260 300 hundred million yuan, and for the economic loss making up various disease and ecological deterioration brings, chemosynthesis material and antibiotic are widely used。Research shows, life-time service antibiotic, the residual of medicine, cause of disease drug resistance can be caused to strengthen, host immune is tired, breeding environment worsens and brings the negative effects such as adverse consequences to human health, the U.S. and some countries of West Europe disable or limit the use of antibiotic in succession as feed additive, therefore develop have no side effect, safe and reliable, multi-functional, noresidue, feed additive free from environmental pollution are the key points solving the problems referred to above。

Summary of the invention

The technical problem to be solved in the present invention is to provide a kind of microbial forage additive and preparation method thereof, it mainly adopts the microbial forage additive containing bacillus subtilis that biological bacteria is prepared from, have have no side effect, safe and reliable, multi-functional, noresidue, distinguishing feature free from environmental pollution。

In order to solve above-mentioned technical problem, the present invention adopts following technical proposals to realize:

A kind of feed additive containing bacillus subtilis, it is mainly prepared from by following raw materials in parts by weight number ratio: bacillus subtilis 15-20 part, Laetiporus sulphureus (Bull. Ex Fr.) Bond. Et Singer. 5-15 part, Lentinus Edodes fungus 5 15 parts, Auricularia fungus 5 15 parts, hericium erinaceus 5 15 parts, willow mushroom 5 15 parts。

Described a kind of feed additive containing bacillus subtilis, it is mainly prepared from by following raw materials in parts by weight number ratio: bacillus subtilis 15 parts, Laetiporus sulphureus (Bull. Ex Fr.) Bond. Et Singer. 10 parts, Lentinus Edodes fungus 10 parts, Auricularia fungus 10 parts, hericium erinaceus 5 parts, willow mushroom 5 parts。

The preparation method of described a kind of feed additive containing bacillus subtilis, take the bacillus subtilis meeting described parts by weight of drying, Laetiporus sulphureus (Bull. Ex Fr.) Bond. Et Singer., Lentinus Edodes fungus, willow mushroom carries out coarse pulverization and obtains dry raw material, add the dry raw material of hot-water soak of dry 60 120 times of weight of raw material weight, soaking temperature is 70 DEG C, soak time is 8 10 hours, it is filtered afterwards, after filtration, filtrate is standby, filtering residue adds ethanol stirring, the addition of ethanol is that every 1 kilogram of filtering residue adds 140-160 gram, the hot-water soak being stirring evenly and then adding into 40 60 times of weight of filtering residue carries out second extraction, soaking temperature is 70 DEG C, soak time is 10 16 hours, after again filtering, merge twice filtrate, filtrate after merging adds the Auricularia fungus of described parts by weight, hericium erinaceus, carry out heated at constant temperature again, heating-up temperature is 70 DEG C, heat 12 24 hours, it is filtered afterwards, fluid purification after filtering, clarification time is 24 48 hours, eventually pass 95 DEG C of 110 DEG C of high temperature sterilizes, sterilization time is 60 90 minutes, obtain described a kind of feed additive。

Advantages of the present invention and effect are as follows:

One microbial forage additive of the present invention produces and using method is easy, safely and effectively。A large amount of feeding trial is carried out in areas such as the Shandong Zibo of China, the Taizihe District in Liaoning, the Inner Mongol through inventor, result of study shows, a kind of microbial forage additive of the present invention has the various harmful toxins effects contained in anti-multiple disease, anti-pestilence, the immunity that improves, degraded feedstuff, additive and vaccine。The present invention adopts the wild microbial fungi of pure natural to form through artificial domesticating cultivation, is a kind of material contributing to intestinal microbial balance。It is containing physiologically active bacterium, can pass through gastrointestinal or field planting is bred in intestinal, adjusts intestinal microbial population thus improving the microorganism of the work of immunity of organisms。The present invention is a kind of rich in macronutrient, research shows, after the present invention enters livestock and poultry alimentary tract with the form of spore, breeding can be sprouted rapidly in upper intestines and produce highly active digestive enzyme, contribute to the crude protein in feedstuff and the digest and decompose of some of complex carbohydrate, promote animal digesting and assimilating nutrient substance, promote growth。The present invention can change the micropopulation in host, thus being substantially reduced pathogen to occupy the chance of intestinal environment, it is possible to draw pathogenic bacteria growth and breeding。

A kind of microbial forage additive of the present invention have composite flora, effect substantially, have no side effect, safe and reliable, multi-functional, noresidue, distinguishing feature free from environmental pollution。

Detailed description of the invention

Below in conjunction with example and experimental example, the present invention is described in further details, but protection scope of the present invention is not limit by embodiment。

Embodiment 1:

A kind of feed additive containing bacillus subtilis of the present invention, its raw material used is as follows: bacillus subtilis 15 parts, Laetiporus sulphureus (Bull. Ex Fr.) Bond. Et Singer. 10 parts, Lentinus Edodes fungus 10 parts, Auricularia fungus 10 parts, hericium erinaceus 5 parts, willow mushroom 5 parts。

A kind of feed addictive preparation method containing bacillus subtilis is as follows, take the bacillus subtilis meeting described parts by weight of drying, Laetiporus sulphureus (Bull. Ex Fr.) Bond. Et Singer., Lentinus Edodes fungus, willow mushroom carries out coarse pulverization and obtains dry raw material, add the dry raw material of hot-water soak of dry 60 times of weight of raw material weight, soaking temperature is 70 DEG C, soak time is 8 hours, it is filtered afterwards, after filtration, filtrate is standby, filtering residue adds ethanol stirring, the addition of ethanol is that every 1 kilogram of filtering residue adds 140 grams, the hot-water soak being stirring evenly and then adding into 40 times of weight of filtering residue carries out second extraction, soaking temperature is 70 DEG C, soak time is 10 hours, after again filtering, merge twice filtrate, filtrate after merging adds the Auricularia fungus of described parts by weight, Hericium erinaceus (Bull. Ex Fr.) Pers., carry out heated at constant temperature again, degree of heating is 70 DEG C, soak 12 hours, it is filtered, fluid purification after filtering, clarification time is 24 hours, eventually pass 95 DEG C of high temperature sterilizes, sterilization time is 60 minutes, obtain described a kind of microbial forage additive。

Embodiment 2:

One microbial forage additive of the present invention, its raw material used is as follows: bacillus subtilis 18 parts, Laetiporus sulphureus (Bull. Ex Fr.) Bond. Et Singer. 5 parts, Lentinus Edodes fungus 5 parts, Auricularia fungus 5 parts, hericium erinaceus 10 parts, willow mushroom 10 parts。

The preparation method of microbial forage additive is as follows, take the bacillus subtilis meeting described parts by weight of drying, Laetiporus sulphureus (Bull. Ex Fr.) Bond. Et Singer., Lentinus Edodes fungus, willow mushroom carries out coarse pulverization and obtains dry raw material, add the dry raw material of hot-water soak of dry 120 times of weight of raw material weight, soaking temperature is 70 DEG C, soak time is 10 hours, it is filtered afterwards, after filtration, filtrate is standby, filtering residue adds ethanol stirring, the addition of ethanol is that every 1 kilogram of filtering residue adds 160 grams, the hot-water soak being stirring evenly and then adding into 60 times of weight of filtering residue carries out second extraction, soaking temperature is 70 DEG C, soak time is 16 hours, after again filtering, merge twice filtrate, filtrate after merging adds the Auricularia fungus of described parts by weight, Hericium erinaceus (Bull. Ex Fr.) Pers., carry out heated at constant temperature again, degree of heating is 70 DEG C, soak 24 hours, it is filtered, fluid purification after filtering, clarification time is 48 hours, eventually pass 110 DEG C of high temperature sterilizes, sterilization time is 90 minutes, obtain described a kind of microbial forage additive。

Embodiment 3:

One microbial forage additive of the present invention, its raw material used is as follows: bacillus subtilis 20 parts, Laetiporus sulphureus (Bull. Ex Fr.) Bond. Et Singer. 15 parts, Lentinus Edodes fungus 15 parts, Auricularia fungus 15 parts, hericium erinaceus 15 parts, willow mushroom 15 parts。

The preparation method of microbial forage additive is as follows, take the bacillus subtilis meeting described parts by weight of drying, Laetiporus sulphureus (Bull. Ex Fr.) Bond. Et Singer., Lentinus Edodes fungus, willow mushroom carries out coarse pulverization and obtains dry raw material, add the dry raw material of hot-water soak of dry 100 times of weight of raw material weight, soaking temperature is 70 DEG C, soak time is 9 hours, it is filtered afterwards, after filtration, filtrate is standby, filtering residue adds ethanol stirring, the addition of ethanol is that every 1 kilogram of filtering residue adds 150 grams, the hot-water soak being stirring evenly and then adding into 50 times of weight of filtering residue carries out second extraction, soaking temperature is 70 DEG C, soak time is 13 hours, after again filtering, merge twice filtrate, filtrate after merging adds the Auricularia fungus of described parts by weight, Hericium erinaceus (Bull. Ex Fr.) Pers., carry out heated at constant temperature again, degree of heating is 70 DEG C, soak 18 hours, it is filtered, fluid purification after filtering, clarification time is 35 hours, eventually pass 100 DEG C of high temperature sterilizes, sterilization time is 70 minutes, obtain described a kind of microbial forage additive。

Claims

1. the feed additive containing bacillus subtilis, it is characterised in that it is mainly prepared from by following raw materials in parts by weight number ratio: bacillus subtilis 15-20 part, Laetiporus sulphureus (Bull. Ex Fr.) Bond. Et Singer. 5-15 part, Lentinus Edodes fungus 5 15 parts, Auricularia fungus 5 15 parts, hericium erinaceus 5 15 parts, willow mushroom 5 15 parts。

2. a kind of feed additive containing bacillus subtilis according to claim 1, it is characterised in that it is mainly prepared from by following raw materials in parts by weight number ratio: bacillus subtilis 15 parts, Laetiporus sulphureus (Bull. Ex Fr.) Bond. Et Singer. 10 parts, Lentinus Edodes fungus 10 parts, Auricularia fungus 10 parts, hericium erinaceus 5 parts, willow mushroom 5 parts。

3. the Preparation Method of a kind of feed additive containing bacillus subtilis according to claim 1 and 2, it is characterised in that:

Take the bacillus subtilis meeting described parts by weight of drying, Laetiporus sulphureus (Bull. Ex Fr.) Bond. Et Singer., Lentinus Edodes fungus, willow mushroom carries out coarse pulverization and obtains dry raw material, add the dry raw material of hot-water soak of dry 60 120 times of weight of raw material weight, soaking temperature is 70 DEG C, soak time is 8 10 hours, it is filtered afterwards, after filtration, filtrate is standby, filtering residue adds ethanol stirring, the addition of ethanol is that every 1 kilogram of filtering residue adds 140-160 gram, the hot-water soak being stirring evenly and then adding into 40 60 times of weight of filtering residue carries out second extraction, soaking temperature is 70 DEG C, soak time is 10 16 hours, after again filtering, merge twice filtrate, filtrate after merging adds the Auricularia fungus of described parts by weight, hericium erinaceus, carry out heated at constant temperature again, heating-up temperature is 70 DEG C, heat 12 24 hours, it is filtered afterwards, fluid purification after filtering, clarification time is 24 48 hours, eventually pass 95 DEG C of 110 DEG C of high temperature sterilizes, sterilization time is 60 90 minutes, obtain described a kind of feed additive。