CN108611300A - A kind of saliva Bacillus acidi lactici SNK-6 and its application - Google Patents
A kind of saliva Bacillus acidi lactici SNK-6 and its application Download PDFInfo
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- CN108611300A CN108611300A CN201810447933.3A CN201810447933A CN108611300A CN 108611300 A CN108611300 A CN 108611300A CN 201810447933 A CN201810447933 A CN 201810447933A CN 108611300 A CN108611300 A CN 108611300A
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- bacillus acidi
- acidi lactici
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- saliva bacillus
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- 210000003296 saliva Anatomy 0.000 title claims abstract description 42
- 241000193830 Bacillus <bacterium> Species 0.000 title claims abstract description 39
- 241000287828 Gallus gallus Species 0.000 claims abstract description 16
- 230000000968 intestinal effect Effects 0.000 claims abstract description 5
- 235000016709 nutrition Nutrition 0.000 claims description 3
- 230000035764 nutrition Effects 0.000 claims description 3
- 235000013305 food Nutrition 0.000 claims description 2
- 241000186869 Lactobacillus salivarius Species 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 241001465754 Metazoa Species 0.000 description 5
- 239000003651 drinking water Substances 0.000 description 4
- 239000003814 drug Substances 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 239000001963 growth medium Substances 0.000 description 4
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 3
- 235000020188 drinking water Nutrition 0.000 description 3
- 210000001035 gastrointestinal tract Anatomy 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 238000004321 preservation Methods 0.000 description 3
- 239000007921 spray Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 210000000991 chicken egg Anatomy 0.000 description 2
- 235000013376 functional food Nutrition 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 235000014655 lactic acid Nutrition 0.000 description 2
- 239000004310 lactic acid Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 239000002068 microbial inoculum Substances 0.000 description 2
- 239000002504 physiological saline solution Substances 0.000 description 2
- 238000005507 spraying Methods 0.000 description 2
- 241000304886 Bacilli Species 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 101000836873 Homo sapiens Nucleotide exchange factor SIL1 Proteins 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- 102100027096 Nucleotide exchange factor SIL1 Human genes 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 241000219000 Populus Species 0.000 description 1
- 241000607142 Salmonella Species 0.000 description 1
- 101000880156 Streptomyces cacaoi Subtilisin inhibitor-like protein 1 Proteins 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000000675 anti-caries Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 235000012206 bottled water Nutrition 0.000 description 1
- 210000000481 breast Anatomy 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 229940099352 cholate Drugs 0.000 description 1
- BHQCQFFYRZLCQQ-OELDTZBJSA-N cholic acid Chemical compound C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(O)=O)C)[C@@]2(C)[C@@H](O)C1 BHQCQFFYRZLCQQ-OELDTZBJSA-N 0.000 description 1
- 238000003501 co-culture Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000000378 dietary effect Effects 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000001408 fungistatic effect Effects 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 210000003405 ileum Anatomy 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000010871 livestock manure Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 238000000879 optical micrograph Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 210000000813 small intestine Anatomy 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 210000005239 tubule Anatomy 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 230000003442 weekly effect Effects 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/225—Lactobacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
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- Genetics & Genomics (AREA)
- Animal Husbandry (AREA)
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- Biochemistry (AREA)
- Biotechnology (AREA)
- Environmental Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Birds (AREA)
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- General Health & Medical Sciences (AREA)
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Abstract
The invention discloses a kind of saliva Bacillus acidi lactici SNK 6 and its applications, wherein the preserving number of the saliva Bacillus acidi lactici SNK 6 is CCTCC NO：M 2018044,16SDNA sequence such as SEQ ID NO:Shown in 1, and it is separating obtained from chicken intestinal, above-mentioned saliva Bacillus acidi lactici SNK 6 is avoiding egg from generating blackening, is improving the application on egg quality.
Description
Technical field
The invention belongs to microorganisms technical fields, and in particular to a kind of saliva Bacillus acidi lactici SNK-6 and its application.
Background technology
Currently, the approach for improving egg quality mainly has：Using the good layer breed of Egg Quality, high-quality feed is fed, with
And the production environment for controllably meeting laying hen needs and preferable egg Conservation environment are provided, also some researches show that added in feed
Traditional Chinese medicine ingredients can change egg ingredients.But the difference on effect for improving egg quality by above method is larger, to reducing chicken
Egg blackening is all without substantial effect or effect unobvious.The effect for improving egg quality by genetic and breeding method is slower, leads to
Egg blackening can't be avoided completely by crossing adjustment feed nutrient；In addition, improving egg quality using Chinese medicine has certain effect
Fruit, but effect is not notable, also is not used to avoid the relevant report of egg blackening about Chinese medicine.
CN106399197A discloses a kind of Lactobacillus salivarius and application thereof, is used for anticaries；CN103289926B is public
One plant of lactobacillus salivarius strains and its application have been opened, Xinjiang, China Mountain Ranges of Tian Shan Mountains stage casing is isolated from, it is uninhabited, dense vegetation
Earth's surface humic soil layer is separately cultured and is inoculated with by the way that bacterial multiplication is enriched in MRS liquid selective mediums by wild chicken manure
Purifying obtains, the application in preparing the drug for adjusting microbial population of animal intestinal tract, promotion growth of animal；
CN102061272B discloses lactobacillus salivarius strain SIL1 and its application, derive from weanling pig, to acid, cholate and
Temperature has good tolerance, can be grown in simulating gastro-intestinal Fluid, the Escherichia coli to co culture system in vitro and salmonella tool
There is 100% fungistatic effect, can be colonized in animal intestinal tract, there is the work(for adjusting microbial population of animal intestinal tract distribution and promoting growth of animal
Energy.But there is presently no the Lactobacillus salivarius detached from chicken intestinal, also avoid egg blackening not over Lactobacillus salivarius
Correlative study and application report.
Invention content
The present inventor has found afterwards after extensive and in-depth study, generates the hen of egg blackening and does not generate chicken
For the ileum microorganism group of the hen of egg blackening at there are pole significant difference, wherein saliva Bacillus acidi lactici difference is present in all surveys
In fixed experiment, and the enteric microorganism bacterial strain saliva Bacillus acidi lactici SNK-6 isolated from laying hen enteron aisle is thrown by dietary methods
After feeding laying hen, it can avoid egg that blackening occurs, while egg hangh unit can be improved.Based on above-mentioned discovery, the present invention carries
A kind of saliva Bacillus acidi lactici SNK-6 detached from chicken intestinal has been supplied, above-mentioned saliva Bacillus acidi lactici SNK-6 is also provided and is avoiding chicken
Egg generates blackening, improves the application in egg quality.
The above-mentioned purpose of the present invention is achieved through the following technical solutions：
A kind of saliva Bacillus acidi lactici SNK-6, preserving number are CCTCC NO：M 2018044.
Preferably, the 16SDNA sequences such as SEQ ID NO of above-mentioned saliva Bacillus acidi lactici SNK-6:Shown in 1.
Preferably, the saliva Bacillus acidi lactici SNK-6 is separating obtained from chicken intestinal.
Another aspect of the present invention also resides in above-mentioned saliva Bacillus acidi lactici SNK-6 and is avoiding egg from generating blackening, improving chicken
Application in Egg Quality.
In a preferred embodiment of the invention, a kind of functional food stuff for chicken laying includes above-mentioned saliva Bacillus acidi lactici SNK-6,
Further include common chicken feed nutrition component or carrier；Wherein, common chicken feed nutrition component or carrier include corn, dregs of beans, mine
Substance and vitamin.The preparation process of above-mentioned functionality food stuff for chicken laying is as follows：First the saliva Bacillus acidi lactici SNK-6 is added to
Mixing in physiological saline, it is preferred that be made into 1L normal saline solutions by 1g saliva Bacillus acidi lacticis SNK-6；It is raised again in conventional hen
Sprayed on material above-mentioned saliva Bacillus acidi lactici SNK-6 microbial inoculums solution to get.Following standardization feeding manner is pressed again raises laying hen：First
Hen is raised in a standardized manner, is fed within the 2nd day above-mentioned functional food stuff for chicken laying, is then fed above-mentioned function every repetition in 7 days
Sexupara chicken feed；Preferably, feeding volume feeds the physiological saline of the 1L Bacillus acidi lacticis of saliva containing 1g SNK-6 according to 1000 hens.
In a preferred embodiment of the invention, laying hen drinking water includes above-mentioned saliva Bacillus acidi lactici SNK-6, further includes drink
Use water；Laying hen is raised by following standardization feeding manner：Hen is first raised in a standardized manner, and the 2nd day in hen potable water tank
Above-mentioned saliva Bacillus acidi lactici SNK-6 is added, then feeds above-mentioned hen drinking water every repetition in 7 days；Preferably, feeding volume according to
1000 hens feed the drinking water of the 1L Bacillus acidi lacticis of saliva containing 1g SNK-6.
In a preferred embodiment of the invention, laying hen henhouse spraying includes above-mentioned saliva Bacillus acidi lactici SNK-6, further includes
Water；Laying hen is raised by following standardization feeding manner：Hen is raised in a standardized manner, is uniformly sprayed in laying hen henhouse within the 2nd day
The spraying for including above-mentioned saliva Bacillus acidi lactici SNK-6 is spilt, then every 7 days repetition aforesaid operations；Preferably, fountain height according to
The water spray of the 1000 hen sprinkling 1L Bacillus acidi lacticis of saliva containing 1g SNK-6.
Compared with prior art, the beneficial effects of the present invention are：Pass through the saliva lactic acid bar to the laying hen feeding present invention
Bacterium SNK-6, can be to avoid egg blackening, and egg quality is significantly better than the hen for not feeding saliva Bacillus acidi lactici SNK-6.
Description of the drawings
Fig. 1 is the Gram stain figure of saliva Bacillus acidi lactici SNK-6 of the present invention；
Biomaterial preservation information：
Saliva Bacillus acidi lactici SNK-6, Classification And Nomenclature are：Lactobacillus Ralivarius SNK-6, in 2018
17 days 01 month in the China typical culture collection center preservation positioned at Wuhan City, Hubei Province Wuchang District Wuhan University in the school, preservation
Number is：CCTCC NO：M 2018044.
Specific implementation mode
The technical solution that the invention will now be described in detail with reference to the accompanying drawings, but protection scope of the present invention is not limited to following realities
Example is applied, the experimental method in following embodiment is conventional method unless otherwise specified, and reagent is with material without the equal city of specified otherwise
Selling can obtain.
The separation of 1 saliva Bacillus acidi lactici SNK-6 of embodiment
First observe egg blackening, finding out production egg does not have blackening or the less hen of blackening, prepare special culture media and
10cm tablets 5；Prepare 2mL centrifuge tubes again, the above-mentioned special culture medias of 1mL are added, with the fresh small intestine source of cotton swab acquisition hen
It in excrement 1g to the above-mentioned centrifuge tube equipped with special culture media, waves 10 times, mixing stands 1 minute；Then upper solution is drawn
To another 2mL centrifuge tubes, supplementing culture medium to 1.5mL waves 10 times, and mixing stands 1 minute, and it is primary to repeat the operation.
The upper layer culture medium of 1mL is assigned to 5 tablets again, coated plate is cultivated 24 hours for 42 DEG C in anaerobic culture box, chosen
It takes and protrudes apparent shallow white colony, optical microphotograph sem observation after Gram stain, as shown in Fig. 1；Take the doubtful saliva breast of form
The bacterium colony tubule Anaerobic culturel of acidfast bacilli SNK-6 13 hours is used in combination PCR to detect 16sDNA sequence verifications, as a result such as sequence table SEQ
ID NO:Shown in 1.
2 saliva Bacillus acidi lactici SNK-6 raising laying hen experiments of embodiment
It selects the black plumage laying hen of new poplar to do feeding experiment, and is divided into two groups, one of which laying hen is by standardization raising side
Formula is raised；Another group while standardizing feeding manner raising, by 1000 hen 1g saliva Bacillus acidi lactici SNK-6, is made into
1000mL normal saline solutions are sprayed on feed surface after positive feed using watering can, and repetition in second day is sprayed primary, continuously sprays saliva
After 3 weeks, the fresh egg of 4th week starts not generate blackening Bacillus acidi lactici SNK-6 bacterial strains.It subsequently sprays weekly and states saliva lactic acid bar
Bacterium SNK-6 microbial inoculums 1 time, fresh egg does not generate blackening.Test the egg blackening incidence given birth to and hangh unit result such as 1 institute of table
Show, it can be seen that compared with the saliva Bacillus acidi lactici SNK-6 raising for not adding the present invention, the present invention can to avoid egg blackening,
And egg quality is significantly better than the hen for not feeding saliva Bacillus acidi lactici.
Table 1
| Group | Blackening incidence | Store 7 days egg hangh units | Store 14 days egg hangh units |
| Addition group | 0% | 83 | 79 |
| Do not add group | 60% | 80 | 75 |
The above is presently preferred embodiments of the present invention, but the present invention should not be limited to disclosed in the embodiment
Content.So every do not depart from the lower equivalent or modification completed of spirit disclosed in this invention, the model that the present invention protects is both fallen within
It encloses.
Sequence table
<110>Academy of Agricultural Sciences, Shanghai City
<120>A kind of Lactobacillus salivarius SNK-6 and its application
<130> 2018-05-02
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1432
<212> DNA
<213>Unknown ()
<400> 1
ttagacggct ggctccttgc ggttacccca ccggctttgg gtgttacaaa ctctcatggt 60
gtgacgggcg gtgtgtacaa ggcccgggaa cgtattcacc gcgacatgct gattcgcgat 120
tactagcgat tccgacttca tgtaggcgag ttgcagccta caatccgaac tgagaacggc 180
tttaagagat tagctaaacc tcgcggtctt gcgactcgtt gtaccgtcca ttgtagcacg 240
tgtgtagccc aggtcataag gggcatgatg acttgacgtc gtccccacct tcctccggtt 300
tgtcaccggc agtctcgcca gagtgcccaa cttaatgctg gcaactgaca acaagggttg 360
cgctcgttgc gggacttaac ccaacatctc acgacacgag ctgacgacag ccatgcacca 420
cctgtcactt tgtccccgaa gggaaagcct aatctcttag gtggtcaaag gatgtcaaga 480
cctggtaagg ttcttcgcgt tgcttcgaat taaaccacat gctccaccgc ttgtgcgggc 540
ccccgtcaat tcctttgagt ttcaaccttg cggtcgtact ccccaggcgg aatgcttatt 600
gcgttagctg cggcactgaa gggcggaaac cctccaacac ctagcattca tcgtttacgg 660
cgtggactac cagggtatct aatcctgttt gctacccacg ctttcgaacc tcagcgtcag 720
ttacagacca gagagccgct ttcgccactg gtgttcttcc atatatctac gcatttcacc 780
gctacacatg gagttccact ctcctcttct gcactcaagt cttccagttt ccaatgcact 840
actccggtta agccgaaggc tttcacatca gacttaaaag accgcctgcg ttccctttac 900
gcccaataaa tccggacaac gcttgccacc tacgtattac cgcggctgct ggcacgtagt 960
tagccgtgac ttgctggtta gataccgtca tcgaatgaac agttactctc actcgtgttc 1020
ttctctaaca acagagtttt acgatccgaa gaccttcttc actcacgcgg cgttgctcca 1080
tcagacttgc gtccattgtg gaagattccc tactgctgcc tcccgtagga gtttgggccg 1140
tgtctcagtc ccaatgtggc cgatcaacct ctcagttcgg ctacgtatca tcaccttggt 1200
aggccgttac cccaccaact agttaatacg ccgcgggtcc atctaaaagc gatagcagaa 1260
ccatctttca tctaaggatc atgcgatcct tagagatata cggtattagc acctgtttcc 1320
aagtgttatc cccttctttt aggcaggtta cccacgtgtt actcacccgt ccgccactca 1380
acttcttacg gtgaatgcaa gcattcggtg taagaaagtt tcgtcgactg ca 1432
Claims (5)
1. a kind of saliva Bacillus acidi lactici SNK-6, preserving number is CCTCC NO：M 2018044.
2. saliva Bacillus acidi lactici SNK-6 as described in claim 1, which is characterized in that 16SDNA sequences such as SEQ IDNO:1 institute
Show.
3. saliva Bacillus acidi lactici SNK-6 as described in claim 1, which is characterized in that separating obtained from chicken intestinal.
4. a kind of functionality food stuff for chicken laying, which is characterized in that including the saliva Bacillus acidi lactici as described in claim any one of 1-3
SNK-6 further includes common chicken feed nutrition component or carrier.
5. saliva Bacillus acidi lactici SNK-6 is avoiding egg from generating blackening, is improving egg quality as described in claim any one of 1-3
On application.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810447933.3A CN108611300B (en) | 2018-05-11 | 2018-05-11 | Lactobacillus salivarius SNK-6 and application thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810447933.3A CN108611300B (en) | 2018-05-11 | 2018-05-11 | Lactobacillus salivarius SNK-6 and application thereof |
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| Publication Number | Publication Date |
|---|---|
| CN108611300A true CN108611300A (en) | 2018-10-02 |
| CN108611300B CN108611300B (en) | 2021-04-20 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112021468A (en) * | 2020-08-26 | 2020-12-04 | 浙江大学 | Additive for improving laying rate and egg quality of laying hens and preparation and application thereof |
| CN112293345A (en) * | 2020-11-16 | 2021-02-02 | 上海市农业科学院 | Laying hen feeding method capable of preventing eggs from producing freckles |
| CN113789281A (en) * | 2021-09-18 | 2021-12-14 | 江苏农林职业技术学院 | Probiotics for improving egg laying performance and egg quality and application thereof |
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| WO2003071883A1 (en) * | 2002-02-28 | 2003-09-04 | Centro Sperimentale Del Latte S.P.A. | Dietetic and/or pharmaceutical compositions for human and/or animal use based on probiotic microbial preparations |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112021468A (en) * | 2020-08-26 | 2020-12-04 | 浙江大学 | Additive for improving laying rate and egg quality of laying hens and preparation and application thereof |
| CN112293345A (en) * | 2020-11-16 | 2021-02-02 | 上海市农业科学院 | Laying hen feeding method capable of preventing eggs from producing freckles |
| CN113789281A (en) * | 2021-09-18 | 2021-12-14 | 江苏农林职业技术学院 | Probiotics for improving egg laying performance and egg quality and application thereof |
| CN113789281B (en) * | 2021-09-18 | 2023-04-14 | 江苏农林职业技术学院 | Probiotics for improving egg laying performance and egg quality and application thereof |
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