CN105662946A - Pomegranate peel extract preparation method and application of pomegranate peel extract to cosmetics - Google Patents
Pomegranate peel extract preparation method and application of pomegranate peel extract to cosmetics Download PDFInfo
- Publication number
- CN105662946A CN105662946A CN201610035564.8A CN201610035564A CN105662946A CN 105662946 A CN105662946 A CN 105662946A CN 201610035564 A CN201610035564 A CN 201610035564A CN 105662946 A CN105662946 A CN 105662946A
- Authority
- CN
- China
- Prior art keywords
- solution
- extraction
- phase
- pericarpium granati
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Dermatology (AREA)
- Microbiology (AREA)
- Epidemiology (AREA)
- Birds (AREA)
- Mycology (AREA)
- Botany (AREA)
- Biotechnology (AREA)
- Engineering & Computer Science (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Cosmetics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention provides a pomegranate peel extract preparation method and application of pomegranate peel extract to cosmetics.The pomegranate peel extract preparation method includes the steps of firstly, concentrating, namely weighing ground pomegranate peel, adding an ethanol solution prior to ultrasonic-assisted extraction, filtering and performing vacuum concentration to obtain a concentrated solution; secondly, extracting, namely subjecting the concentrated solution to ethyl acetate extraction and sodium hydroxide solution extraction sequentially so as to obtain extraction liquid A; thirdly, precipitating, namely adding a gelatin solution into the extraction liquid A, standing, filtering to obtain precipitate, dissolving the precipitate by an acetone solution, filtering to remove undissolved substances, and recovering acetone from filtrate to obtain residual liquid; fourthly, separating and purifying the residual liquid.The pomegranate peel extract is light in color, high in polyphenol content and remarkable in antioxidant activity and can be applied to the field of cosmetics.A skin-moisturizing anti-wrinkle pomegranate cream prepared by the pomegranate peel extract is capable of delaying senescence, reducing wrinkles, moisturizing the skin, caring the skin and the like when being used on the surface of the skin.
Description
Technical field
The present invention relates to cosmetic field, it is specifically related to the preparation of a kind of Pericarpium Granati extract and the application in makeup thereof.
Background technology
Pomegranate (formal name used at school: PunicagranatumL.) deciduous tree or shrub; Dan Ye, usually to giving birth to or cluster, without stipule. Hua Dingsheng or closely push up life, single raw or several cluster or form cyme, closely bell, sliver 5-9, petal 5-9, many gauffers, imbricate arrangement; Ovule is most. Berry is spherical, and top has place to deposit calyx sliver, and pericarp is thick; Seed is most, and berry is closely spherical, the fructescence 9-10 month. Exosper meat is translucent, succulence; Inner seed coat leather matter. Nature and flavor are sweet, sour and astringent, warm, have effects such as killing worm, convergence, puckery intestines, only dysentery.
Pomegranate is a kind of berry, and it is nutritious, and vitamins C is higher 1～1 times than apple, pears. Originating in the China Western Regions, Han dynasty spreads out of Central Plains. After pomegranate maturation, whole body all can be used, and pericarp can be used as medicine, and edible fruit is used or pressure juice. Healthy to the elderly has very high nutritive value, so old man should often eat pomegranate. Pomegranate is a kind of rare berry, and its fruits nutrition is worth higher. Research finds, pomegranate is containing mineral substance such as a large amount of organic acids, carbohydrate, protein, fat, VITAMIN and calcium, phosphorus, potassium. The traditional Chinese medical science is thought, pomegranate tool heat-clearing, removing toxic substances, flat liver, enriches blood, invigorates blood circulation and antidiarrheal efficacy, is applicable to icterohepatitis, asthma and the patient rushed down for a long time and the women excessively grown menstrual period.
Pomegranate rind is the dry pericarp of pomegranate, containing multiple alkaloid, bacteriostatic test confirms, the alcohol extract of pomegranate and pericarp water decoction, having broad-spectrum antibacterial action, streptococcus aureus, Hemolytic streptococcus, vibrio cholerae, dysentery bacterium etc. are had obvious restraining effect by it, wherein that dysentery bacterium effect is the strongest, various dermatophytes is also had restraining effect in various degree in vitro by pomegranate rind water logging agent, and pomegranate rind decoction can also suppress influenza virus.
In prior art, patent CN101057878A discloses a kind of Pericarpium Granati extract and its preparation method, pomegranate rind is extracted as extraction solvent through low-carbon alcohol, concentrated, column chromatography, concentrated, dry, obtain powder shape Pericarpium Granati extract. But, the method products obtained therefrom color and luster is darker.
Summary of the invention
For defect of the prior art, it is an object of the invention to provide the preparation of a kind of Pericarpium Granati extract and the application in makeup thereof.Products obtained therefrom look shallow, and in faint yellow, polyphenol content height, anti-oxidant activity is remarkable, can be used as natural antioxidants for cosmetic field.
The present invention is that one extracts oxidation-resistant active ingredient in red pomegranate rind based on solvent-extraction process, and adopts polymeric amide chromatography to carry out the method for the red Pericarpium Granati extract of separation and purification.
It is an object of the invention to be achieved through the following technical solutions:
The present invention provides the preparation method of a kind of Pericarpium Granati extract, and described method comprises the steps:
Extraction step: take pomegranate rind crushed material, add ethanolic soln, ultrasonic-assisted extraction, filters, then concentrating under reduced pressure, obtains concentrated solution;
Extraction step: concentrated solution is extracted through extraction into ethyl acetate and sodium hydroxide solution successively, obtains extraction liquid A;
Settling step: add gelatin solution in extraction liquid A, leaves standstill, and filters, obtains precipitation; By precipitation acetone solution, filtering insolubles; Filtrate obtains residual solution after reclaiming acetone;
Purification procedures: residual solution is carried out separation and purification, to obtain final product.
Preferably, in described extraction step, the solid-to-liquid ratio g/mL of described pomegranate rind crushed material and ethanolic soln is 1:20～1:40, and the volume fraction of ethanolic soln is 50-80%, it is more preferable to the volume fraction of ethanolic soln is 60-75%.
The volumetric concentration of ethanolic soln and solid-liquid ratio are the most remarkable on the extraction yield impact of the oxidation-resistant active ingredient taking polyphenol as representative. In the present invention, ethanol contend mark, between 30%-50%, can significantly improve with the raising polyphenol yield of concentration, and ethanol contend mark is at 50%-80%, and polyphenol variation of yield is little, and ethanol contend mark is higher than 80% rear polyphenol yield decline. Solid-liquid ratio is between 1:20-1:40 scope, and polyphenol yield improves with the increase of solid-liquid ratio, but more than, after 1:30, the impact of polyphenol yield is reduced by solid-liquid ratio gradually.
Preferably, in extraction step, described ultrasonic-assisted extraction 30-60 minute, extract 2 times; More preferably ultrasonic-assisted extraction 40-60 minute,
Preferably, described extraction step is specially: concentrated solution and ethyl acetate equal-volume extracting twice, after extraction liquid merges, reclaims the volume half of ethyl acetate to extraction liquid, it is the sodium hydroxide solution equal-volume extraction of 1-10% again by the extraction liquid after volume half and mass concentration, obtains extraction liquid A.
Preferably, in settling step, described extraction liquid is with dilute hydrochloric acid adjust pH to 4-6, and the mass concentration of gelatin solution is 1-10%; More preferably the mass concentration of gelatin solution is 3-5%.
Preferably, in settling step, the volume fraction of described acetone soln is 40-70%, it is more preferable to the volume fraction of acetone soln is 50-70%.
Preferably, described purification procedures is specially: residual solution is splined on polymeric amide chromatographic column, successively with the 20% of 3-6 times of column volume, 40%, 60%, and dehydrated alcohol wash-out, collect each elutriant respectively, being wherein the elutriant concentrating under reduced pressure of 40% ethanol elution acquisition through volume fraction, lyophilize, obtains Pericarpium Granati extract.
In experimentation, each elutriant is collected respectively, but final is index through polyphenol content mensuration and polyphenol yield two aspect, and retaining 40% ethanol eluate is products obtained therefrom, and 60% ethanol and dehydrated alcohol elution process can count the regenerative process of polymeric amide chromatographic column.
Present invention also offers the Pericarpium Granati extract that the preparation method of a kind of described Pericarpium Granati extract obtains and prepare the purposes of makeup.
Present invention also offers a kind of red pomegranate profit skin anti-wrinkle cream, in massfraction 100%, comprise each component of following massfraction: Pericarpium Granati extract 1-3%, Aloe polysaccharide extracting solution 15-30%, sad and/or Triglyceride DDD 3-6%, stearyl alcohol 3-8%, shea butter 1-3%, Nigella damascena L. oil 1-3%, emulsifying wax 3-6%, butyleneglycol 3-5%, collagen protein 0.5-1%, Acritamer 940 0.2-0.5%, Phenoxyethanol 0.5-0.8%, Sensiva SC50 0.5-0.8%, EDETATE SODIUM 0.05-0.1%, sodium hydroxide 0.2-0.4%, essence 0.3-0.8%, surplus is deionized water.
Present invention also offers the preparation method of a kind of red pomegranate profit skin anti-wrinkle cream, comprise the following steps:
S1, take each component raw material:
A phase: sad and/or Triglyceride DDD, stearyl alcohol, shea butter, Nigella damascena L. oil, emulsifying wax;
B phase: Aloe polysaccharide extracting solution, butyleneglycol, Acritamer 940, EDETATE SODIUM, deionized water;
C phase: red Punica granatum L. extract, collagen protein, Phenoxyethanol, Sensiva SC50, sodium hydroxide, essence;
Prepared by S2, A phase mixed solution: mixed by each for A phase raw material, and heated and stirred is even, obtains A phase mixed solution;
Prepared by S3, B phase mixed solution: first so that appropriate amount of deionized water is swelling, Acritamer 940 being obtained solution a, EDETATE SODIUM is dissolved in deionized water and obtains solution b, then solution a, solution b and other B phase raw material is mixed together, and heated and stirred is even, obtains B phase mixed solution;
S4, A phase, B phase, C phase mix: slowly added by B phase mixed solution in A phase mixed solution, homogeneous, stir, after cooling, add each raw material of C phase, then homogeneous, vacuumize, and stir, and cooling, to obtain final product.
Preferably, in described step S2, it is heated to 80 ± 2 DEG C;
In described step S3, it is heated to 70 ± 5 DEG C;
Described step S4 is specially: slowly added by B phase mixed solution in A phase mixed solution, 3000rpm homogeneous 20 minutes, stirs the bubble that disappears, is cooled to 50 ± 5 DEG C, keeping stirring, add each raw material of C phase, 2000rpm homogeneous 20 minutes, vacuumize degassing is steeped, stirring, cooling, to obtain final product.
Compared with prior art, the present invention has following useful effect:
1, to prepare the color of gained Pericarpium Granati extract more shallow in the present invention, and in faint yellow, polyphenol content height, anti-oxidant activity is remarkable, can be used as natural antioxidants for cosmetic field.
2, the red pomegranate profit skin anti-wrinkle cream that prepared by the present invention, for skin surface, has and delays senility, alleviate wrinkle, the effects such as preserving moisture and protecting skin.
Accompanying drawing explanation
By reading with reference to the detailed description that non-limiting example is done by the following drawings, the other features, objects and advantages of the present invention will become more obvious:
Fig. 1 is that different eluted fraction is to the clearance rate of ultra-oxygen anion free radical;
Fig. 2 is that different eluted fraction is to the clearance rate of DPPH free radical.
Embodiment
Below in conjunction with specific embodiment, the present invention is described in detail. The technician contributing to this area is understood the present invention by following examples further, but does not limit the present invention in any form. It should be appreciated that to those skilled in the art, without departing from the inventive concept of the premise, it is also possible to make some distortion and improvement. These all belong to protection scope of the present invention.
Embodiment 1
Present embodiments provide the preparation method of a kind of Pericarpium Granati extract, comprise the following steps:
Step one: extraction step
Being 1:20 by solid-to-liquid ratio g/mL, take pomegranate rind crushed material, adding volume fraction is 60% ethanolic soln, ultrasonic-assisted extraction 40 minutes, filters, and filter residue repeats to extract 1 time, and filtrate merges, concentrating under reduced pressure.
Step 2: extraction step
Concentrated solution merges with equal-volume extraction into ethyl acetate twice, extraction liquid, reclaims ethyl acetate to half volume, continues and extract with equal-volume mass concentration 5% sodium hydroxide solution.
Step 3: settling step
Extraction liquid, with dilute hydrochloric acid adjust pH to 5, adds the gelatin solution that mass concentration is 4%, hold over night, filters; Collecting precipitation dissolves with 50% acetone soln, filtering insolubles, and filtrate reclaims acetone.
Step 4: purification procedures
Filtrate described in step 3 is splined on the polymeric amide chromatographic column that pre-treatment is good, successively with the 20% of 3-6 times of column volume, 40% after reclaiming acetone, 60%, and dehydrated alcohol wash-out, and collect 40% ethanol eluate, concentrating under reduced pressure, lyophilize, obtains Pericarpium Granati extract.
Adopt different eluted fraction: 20%, 40%, 60%, and the Pericarpium Granati extract yield of dehydrated alcohol is respectively 23.6%, 50.1%, 3.3%, and 4.2%, wherein polyphenol content is in massfraction, is respectively 35.2%, 85.5%, 60.2% and 12.1%.
The biological activity assay of embodiment 2 Pericarpium Granati extract
Adopting assay NBT photoreduction and DPPH method to measure the Pericarpium Granati extract adopting different eluted fraction wash-out to obtain in embodiment 1 to the Scavenging activity of ultra-oxygen anion free radical and DPPH free radical respectively, result is shown in Fig. 1, Fig. 2 respectively. When concentration is 2mg/ml, the clearance rate of ultra-oxygen anion free radical is all reached more than 90% by 40% ethanol and 60% ethanol elution flow point; On the Scavenging activity to DPPH free radical, the IC of 40% ethanol elution flow point50Value is 0.02mg/ml, suitable with ascorbic DPPH radical scavenging activity under same experiment condition.
Embodiment 3
Present embodiments provide the preparation method of a kind of Pericarpium Granati extract, comprise the following steps:
Step one: extraction step
Being 1:30 by solid-to-liquid ratio g/mL, take pomegranate rind crushed material, adding volume fraction is 75% ethanolic soln, ultrasonic-assisted extraction 50 minutes, filters, and filter residue repeats to extract 1 time, and filtrate merges, concentrating under reduced pressure.
Step 2: extraction step
Concentrated solution merges with equal-volume extraction into ethyl acetate twice, extraction liquid, reclaims ethyl acetate to half volume, continues and extract with equal-volume mass concentration 4% sodium hydroxide solution.
Step 3: settling step
Extraction liquid, with dilute hydrochloric acid adjust pH to 4, adds the gelatin solution that mass concentration is 5%, hold over night, filters; Collecting precipitation dissolves with 60% acetone soln, filtering insolubles, and filtrate reclaims acetone.
Step 4: purification procedures
Filtrate described in step 3 is splined on the polymeric amide chromatographic column that pre-treatment is good, successively with the 20% of 3-6 times of column volume, 40% after reclaiming acetone, 60%, and dehydrated alcohol wash-out, and collect 40% ethanol eluate, concentrating under reduced pressure, lyophilize, obtains Pericarpium Granati extract.
Adopt different eluted fraction: 20%, 40%, 60%, and the Pericarpium Granati extract yield of dehydrated alcohol is respectively 22.1%, 52.3%, 2.9%, and 3.8%, wherein polyphenol content is in massfraction, is respectively 34.6%, 83.7%, 62.3% and 10.5%.
Embodiment 4
Present embodiments provide the preparation method of a kind of Pericarpium Granati extract, comprise the following steps:
Step one: extraction step
Being 1:40 by solid-to-liquid ratio g/mL, take pomegranate rind crushed material, adding volume fraction is 65% ethanolic soln, ultrasonic-assisted extraction 60 minutes, filters, and filter residue repeats to extract 1 time, and filtrate merges, concentrating under reduced pressure.
Step 2: extraction step
Concentrated solution merges with equal-volume extraction into ethyl acetate twice, extraction liquid, reclaims ethyl acetate to half volume, continues and extract with equal-volume mass concentration 3% sodium hydroxide solution.
Step 3: settling step
Extraction liquid, with dilute hydrochloric acid adjust pH to 6, adds the gelatin solution that mass concentration is 6%, hold over night, filters; Collecting precipitation dissolves with 70% acetone soln, filtering insolubles, and filtrate reclaims acetone.
Step 4: purification procedures
Filtrate described in step 3 is splined on the polymeric amide chromatographic column that pre-treatment is good, successively with the 20% of 3-6 times of column volume, 40% after reclaiming acetone, 60%, and dehydrated alcohol wash-out, and collect 40% ethanol eluate, concentrating under reduced pressure, lyophilize, obtains Pericarpium Granati extract.
Adopt different eluted fraction: 20%, 40%, 60%, and the Pericarpium Granati extract yield of dehydrated alcohol is respectively 24.8%, 54.4%, 3.1%, and 3.6%, wherein polyphenol content is in massfraction, is respectively 30.5%, 87.2%, 56.1% and 9.7%.
Comparative example 1
This comparative example provides the preparation method of a kind of Pericarpium Granati extract, and described preparation method is identical with embodiment 1, and difference is only: in step one, described in the volumes of aqueous ethanol mark that adds be 40%.
Adopt different eluted fraction: 20%, 40%, 60%, and the Pericarpium Granati extract yield of dehydrated alcohol is respectively 33.7%, 46.2%, 3.1%, and 2.8%, wherein polyphenol content is in massfraction, is respectively 22.5%, 75.9%, 60.4% and 13.7%.
Comparative example 2
This comparative example provides the preparation method of a kind of Pericarpium Granati extract, and described preparation method is identical with embodiment 1, and difference is only: in step one, and described solid-to-liquid ratio is 1:10.
Adopt different eluted fraction: 20%, 40%, 60%, and the Pericarpium Granati extract yield of dehydrated alcohol is respectively 21.3%, 45.8%, 10.2%, and 3.6%, wherein polyphenol content is in massfraction, is respectively 38.9%, 82.0%, 58.6% and 12.7%.
Comparative example 3
This comparative example provides the preparation method of a kind of Pericarpium Granati extract, and described preparation method is identical with embodiment 1, and difference is only: in step 3, and described extraction liquid is with dilute hydrochloric acid bar pH value to 7.
Adopt different eluted fraction: 20%, 40%, 60%, and the Pericarpium Granati extract yield of dehydrated alcohol is respectively 18.7%, 35.2%, 18.9%, and 2.7%, wherein polyphenol content is in massfraction, is respectively 32.6%, 75.4%, 53.5% and 10.1%.
The preparation of the red pomegranate profit skin anti-wrinkle cream of embodiment 3
Present embodiments providing a kind of red pomegranate profit skin anti-wrinkle cream and its preparation method, described preparation method is:
A phase: caprylic/capric triglyceride, stearyl alcohol, shea butter, Nigella damascena L. oil, emulsifying wax;
B phase: Aloe polysaccharide extracting solution, butyleneglycol, Acritamer 940, EDETATE SODIUM, deionized water;
C phase: red Punica granatum L. extract, collagen protein, Phenoxyethanol, Sensiva SC50, sodium hydroxide, essence.
The weight part number of above-mentioned each feed composition is as follows:
In weight part number 100%, red Punica granatum L. extract 1-3%, Aloe polysaccharide extracting solution 15-30%, caprylic/capric triglyceride 3-6%, stearyl alcohol 3-8%, shea butter 1-3%, Nigella damascena L. oil 1-3%, emulsifying wax 3-6%, butyleneglycol 3-5%, collagen protein 0.5-1%, Acritamer 940 0.2-0.5%, Phenoxyethanol 0.5-0.8%, Sensiva SC50 0.5-0.8%, EDETATE SODIUM 0.05-0.1%, sodium hydroxide 0.2-0.4%, essence 0.3-0.8%, surplus is deionized water.
Each for A phase raw material is added in beaker, heated and stirred to 80 ± 2 DEG C, treat fully to mix; In B phase raw material, first that Acritamer 940 is fully swelling with appropriate amount of deionized water, EDETATE SODIUM is dissolved in appropriate amount of deionized water in addition, and the two is mixed together with other B phase raw material and stirs evenly, and is heated to 70 ± 5 DEG C; B phase slowly being added A phase, 3000rpm homogeneous 20 minutes, stir the bubble that disappears, be cooled to 50 ± 5 DEG C, keep stirring, add C phase raw material, 2000rpm homogeneous 20 minutes, vacuumize degassing is steeped, and stirs, and cooling, to obtain final product.
Red pomegranate profit skin anti-wrinkle cream prepared by the present embodiment, for skin surface, has and delays senility, alleviate wrinkle, the effects such as preserving moisture and protecting skin.
In sum, the color that the present invention prepares gained Pericarpium Granati extract is more shallow, and in faint yellow, polyphenol content height, anti-oxidant activity is remarkable, can be used as natural antioxidants for cosmetic field.
The above-mentioned description to embodiment can understand and use invention for ease of those skilled in the art. These embodiments obviously easily can be made various amendment by person skilled in the art, and General Principle described herein are applied in other embodiments and need not pass through creative work. Therefore, the invention is not restricted to above-described embodiment, those skilled in the art, according to the announcement of the present invention, do not depart from improvement that category of the present invention makes and amendment all should within protection scope of the present invention.
Claims (10)
1. the preparation method of a Pericarpium Granati extract, it is characterised in that, described method comprises the steps:
Extraction step: take pomegranate rind crushed material, add ethanolic soln, ultrasonic-assisted extraction, filters, then concentrating under reduced pressure, obtains concentrated solution;
Extraction step: concentrated solution is extracted through extraction into ethyl acetate and sodium hydroxide solution successively, obtains extraction liquid A;
Settling step: add gelatin solution in extraction liquid A, leaves standstill, and filters, obtains precipitation; By precipitation acetone solution, filtering insolubles; Filtrate obtains residual solution after reclaiming acetone;
Purification procedures: residual solution is carried out separation and purification, to obtain final product.
2. the preparation method of Pericarpium Granati extract according to claim 1, it is characterised in that, in extraction step, the solid-to-liquid ratio g/mL of described pomegranate rind crushed material and ethanolic soln is 1:20～1:40, and the volume fraction of ethanolic soln is 50-80%.
3. the preparation method of Pericarpium Granati extract according to claim 1, it is characterised in that, in extraction step, described ultrasonic-assisted extraction 30-60 minute, extract 2 times.
4. the preparation method of Pericarpium Granati extract according to claim 1, it is characterized in that, described extraction step is specially: concentrated solution and ethyl acetate equal-volume extracting twice, after gained extraction liquid merges, reclaim the volume half of ethyl acetate to extraction liquid, it is the sodium hydroxide solution equal-volume extraction of 1-10% again by the extraction liquid after volume half and mass concentration, obtains extraction liquid A.
5. the preparation method of Pericarpium Granati extract according to claim 1, it is characterised in that, in settling step, described extraction liquid A is with dilute hydrochloric acid adjust pH to 4-6, and the mass concentration of gelatin solution is 1-10%.
6. the preparation method of Pericarpium Granati extract according to claim 1, it is characterised in that, in settling step, the volume fraction of described acetone soln is 40-70%.
7. the preparation method of Pericarpium Granati extract according to claim 1, it is characterized in that, described purification procedures is specially: residual solution is splined on polymeric amide chromatographic column, successively with the 20% of 3-6 times of column volume, 40%, 60%, with dehydrated alcohol wash-out, collect each elutriant respectively, it is wherein the elutriant concentrating under reduced pressure of 40% ethanol elution acquisition through volume fraction, lyophilize, obtains Pericarpium Granati extract.
8. the Pericarpium Granati extract that the preparation method of a Pericarpium Granati extract according to the arbitrary item of claim 1-7 obtains is in the purposes preparing in makeup.
9. a red pomegranate profit skin anti-wrinkle cream, it is characterized in that, in massfraction 100%, comprise each component of following massfraction: the Pericarpium Granati extract 1-3% prepared according to claim 1, Aloe polysaccharide extracting solution 15-30%, sad and/or Triglyceride DDD 3-6%, stearyl alcohol 3-8%, shea butter 1-3%, Nigella damascena L. oil 1-3%, emulsifying wax 3-6%, butyleneglycol 3-5%, collagen protein 0.5-1%, Acritamer 940 0.2-0.5%, Phenoxyethanol 0.5-0.8%, Sensiva SC50 0.5-0.8%, EDETATE SODIUM 0.05-0.1%, sodium hydroxide 0.2-0.4%, essence 0.3-0.8%, surplus is deionized water.
10. the preparation method of a red pomegranate according to claim 9 profit skin anti-wrinkle cream, it is characterised in that, comprise the following steps:
S1, take each component raw material:
A phase: sad and/or Triglyceride DDD, stearyl alcohol, shea butter, Nigella damascena L. oil, emulsifying wax;
B phase: Aloe polysaccharide extracting solution, butyleneglycol, Acritamer 940, EDETATE SODIUM, deionized water;
C phase: Pericarpium Granati extract, collagen protein, Phenoxyethanol, Sensiva SC50, sodium hydroxide, essence;
Prepared by S2, A phase mixed solution: mixed by each for A phase raw material, and heated and stirred is even, obtains A phase mixed solution;
Prepared by S3, B phase mixed solution: first so that appropriate amount of deionized water is swelling, Acritamer 940 being obtained solution a, EDETATE SODIUM is dissolved in deionized water and obtains solution b, then solution a, solution b and other B phase raw material is mixed together, and heated and stirred is even, obtains B phase mixed solution;
S4, A phase, B phase, C phase mix: slowly added by B phase mixed solution in A phase mixed solution, homogeneous, stir, after cooling, add each raw material of C phase, then homogeneous, vacuumize, and stir, and cooling, to obtain final product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610035564.8A CN105662946B (en) | 2016-01-19 | 2016-01-19 | The preparation of pomegranate rind extract and its application in cosmetics |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610035564.8A CN105662946B (en) | 2016-01-19 | 2016-01-19 | The preparation of pomegranate rind extract and its application in cosmetics |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105662946A true CN105662946A (en) | 2016-06-15 |
| CN105662946B CN105662946B (en) | 2019-03-22 |
Family
ID=56301621
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610035564.8A Active CN105662946B (en) | 2016-01-19 | 2016-01-19 | The preparation of pomegranate rind extract and its application in cosmetics |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105662946B (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106580826A (en) * | 2016-12-21 | 2017-04-26 | 宁波君瑞生物科技有限公司 | Cream with effects of increasing skin moisture and reducing wrinkles |
| CN106666739A (en) * | 2016-12-19 | 2017-05-17 | 黄腾庆 | Preparation method of pomegranate polyphenols |
| CN109908061A (en) * | 2019-04-30 | 2019-06-21 | 郭玲玲 | A kind of fizzy dandruff control shampoo containing and preparation method thereof |
| CN110025552A (en) * | 2019-05-05 | 2019-07-19 | 郭玲玲 | A kind of increase hair tensile strength and the amino acid shampoo of elongation percentage and preparation method thereof |
| CN110652530A (en) * | 2018-06-29 | 2020-01-07 | 伽蓝（集团）股份有限公司 | Application of pomegranate extract |
| WO2021103018A1 (en) * | 2019-11-29 | 2021-06-03 | 陈博浩 | Method for preparing red pomegranate extract and skincare cream comprising same |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100589813C (en) * | 2007-05-30 | 2010-02-17 | 桂林莱茵生物科技股份有限公司 | Pomegranate rind extraction and its preparation method |
| CN103565928A (en) * | 2013-11-01 | 2014-02-12 | 华南农业大学 | Guava hypoglycemic active component, and preparation method and use thereof |
-
2016
- 2016-01-19 CN CN201610035564.8A patent/CN105662946B/en active Active
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106666739A (en) * | 2016-12-19 | 2017-05-17 | 黄腾庆 | Preparation method of pomegranate polyphenols |
| CN106580826A (en) * | 2016-12-21 | 2017-04-26 | 宁波君瑞生物科技有限公司 | Cream with effects of increasing skin moisture and reducing wrinkles |
| CN110652530A (en) * | 2018-06-29 | 2020-01-07 | 伽蓝（集团）股份有限公司 | Application of pomegranate extract |
| CN109908061A (en) * | 2019-04-30 | 2019-06-21 | 郭玲玲 | A kind of fizzy dandruff control shampoo containing and preparation method thereof |
| CN109908061B (en) * | 2019-04-30 | 2022-01-07 | 胡小龙 | Anti-dandruff shampoo rich in foam and preparation method thereof |
| CN110025552A (en) * | 2019-05-05 | 2019-07-19 | 郭玲玲 | A kind of increase hair tensile strength and the amino acid shampoo of elongation percentage and preparation method thereof |
| CN110025552B (en) * | 2019-05-05 | 2022-04-08 | 广州美思生物技术有限公司 | Amino acid shampoo capable of increasing tensile strength and elongation of hair and preparation method thereof |
| WO2021103018A1 (en) * | 2019-11-29 | 2021-06-03 | 陈博浩 | Method for preparing red pomegranate extract and skincare cream comprising same |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105662946B (en) | 2019-03-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105662946A (en) | Pomegranate peel extract preparation method and application of pomegranate peel extract to cosmetics | |
| Pandey et al. | Aloe Vera: A Systematic Review of its Industrial and Ethno-Medicinal Efficacy. | |
| KR101848671B1 (en) | Functional composition for improving hair loss and hair growth comprising plant cell fermentation extract and functional cosmetic comprising the same | |
| EP1302113B1 (en) | Physiologically functional foods and cosmetics containing sphingoglycolipids extracted from konjac for enhancing skin moisture retention and improving skin roughening | |
| CN105061382B (en) | Method for synchronously extracting proanthocyanidin and selenium-enriched protein from black peanut skin | |
| JP5617110B2 (en) | Oral hair growth promoter | |
| CN103564495B (en) | Propolis and cordycepic hypha powder composition, as well as preparation and application thereof | |
| JP3650587B2 (en) | Functional food containing glycosphingolipid and method for producing the same | |
| KR101578464B1 (en) | Antiaging cosmetic composition containing monascus fermented oil | |
| KR101351187B1 (en) | Antioxidant and whitening functional cosmetics | |
| US10806693B2 (en) | Method for increasing lipolysis using a composition comprising bioactive Nelumbo nucifera (lotus) extract | |
| CN111249206A (en) | Anti-oxidation application of tricholoma matsutake extracting solution composition | |
| JP2010229120A (en) | Method for producing maloideae plant leaf extract highly containing phloridzin | |
| KR102252763B1 (en) | Method of Preparing Extract of Red Ginseng Having a Reduced Chromaticity | |
| JP2001131053A (en) | Cosmetic utilizing pomegranate component | |
| CN103504206B (en) | Lipid lowering cake and making method thereof | |
| CN102058731B (en) | Preparation of chaste tree twig leaf and fruit extract and anti-inflammatory medical application thereof | |
| KR102520908B1 (en) | Preparation of aged solution of fruit of dioscorea opposita thumb, and aged solution of fruit of dioscorea opposita thumb prepared thereby | |
| JP7090864B2 (en) | Composition | |
| KR20170091939A (en) | Composition for promoting hair growth or preventing hair loss comprising extract of ginseng floral axis | |
| KR20210016776A (en) | Method of Preparing Concentrate of Deer Antlers | |
| CN109123136A (en) | A kind of pannage improving meat quality | |
| EP1532868B1 (en) | Plant extracts containing Schisandra and Wolfberry and method for the preparation thereof | |
| JP2001321120A (en) | Health food | |
| KR102298659B1 (en) | Cosmetic composition for skin soothing comprising unripe Citrus unshiu extract |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CP01 | Change in the name or title of a patent holder |
Address after: 201612 Building 2, 758 Xinge Road, Xinqiao Town, Songjiang District, Shanghai Patentee after: Shanghai linqingxuan Biotechnology Co., Ltd Address before: 201612 Building 2, 758 Xinge Road, Xinqiao Town, Songjiang District, Shanghai Patentee before: SHANGHAI FOREST CABIN BIOLOGICAL-TECH Co.,Ltd. |
|
| CP01 | Change in the name or title of a patent holder |